MX2012007718A - Aumento de la produccion de metionina mediante la sobreexpresion de succinato deshidrogenasa. - Google Patents

Aumento de la produccion de metionina mediante la sobreexpresion de succinato deshidrogenasa.

Info

Publication number
MX2012007718A
MX2012007718A MX2012007718A MX2012007718A MX2012007718A MX 2012007718 A MX2012007718 A MX 2012007718A MX 2012007718 A MX2012007718 A MX 2012007718A MX 2012007718 A MX2012007718 A MX 2012007718A MX 2012007718 A MX2012007718 A MX 2012007718A
Authority
MX
Mexico
Prior art keywords
succinate dehydrogenase
overexpressing
methionine production
methionine
increasing methionine
Prior art date
Application number
MX2012007718A
Other languages
English (en)
Inventor
Rainer Figge
Original Assignee
Metabolic Explorer Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Metabolic Explorer Sa filed Critical Metabolic Explorer Sa
Publication of MX2012007718A publication Critical patent/MX2012007718A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/12—Methionine; Cysteine; Cystine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/05—Oxidoreductases acting on the CH-CH group of donors (1.3) with a quinone or related compound as acceptor (1.3.5)
    • C12Y103/05001—Succinate dehydrogenase (ubiquinone) (1.3.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/848—Escherichia
    • Y10S435/849—Escherichia coli

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente invención se refiere a un proceso para la mejora de la producción de metionina mediante el cultivo de un microorganismo modificado para aumentar la expresión de genes implicados en la síntesis de succinato deshidrogenada; los microorganismos fueron modificados de tal modo que el rendimiento de metionina/fuente de carbono aumenta; también se reivindica el aislamiento de metionina del medio de fermentación.
MX2012007718A 2009-12-30 2009-12-30 Aumento de la produccion de metionina mediante la sobreexpresion de succinato deshidrogenasa. MX2012007718A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/IB2009/056063 WO2011080542A1 (en) 2009-12-30 2009-12-30 Increasing methionine production by overexpressing succinate dehydrogenase

Publications (1)

Publication Number Publication Date
MX2012007718A true MX2012007718A (es) 2012-07-25

Family

ID=42791079

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2012007718A MX2012007718A (es) 2009-12-30 2009-12-30 Aumento de la produccion de metionina mediante la sobreexpresion de succinato deshidrogenasa.

Country Status (11)

Country Link
US (1) US9267160B2 (es)
EP (1) EP2519643B1 (es)
JP (1) JP5819316B2 (es)
KR (1) KR101770123B1 (es)
CN (1) CN102712941A (es)
AR (1) AR079764A1 (es)
BR (1) BR112012016274B1 (es)
ES (1) ES2654659T3 (es)
MX (1) MX2012007718A (es)
PL (1) PL2519643T3 (es)
WO (1) WO2011080542A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6393898B2 (ja) * 2014-08-03 2018-09-26 国立大学法人 奈良先端科学技術大学院大学 酵母の培養方法
RU2723714C2 (ru) 2014-09-01 2020-06-17 Эвоник Оперейшнс Гмбх Способ и микроорганизм для ферментативного продуцирования метионина с улучшенным выходом метионина
CN108026516A (zh) 2015-08-07 2018-05-11 赢创德固赛有限公司 通过发酵的蛋白硫代羧化物依赖性l-甲硫氨酸生产
EP3380627B1 (en) 2015-11-27 2019-08-14 Evonik Degussa GmbH Method for producing l-methionine
EP3296404A1 (en) 2016-09-15 2018-03-21 Evonik Degussa GmbH Modified microorganism for production of methionine

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000157267A (ja) 1998-11-24 2000-06-13 Ajinomoto Co Inc 変異型ｍｅｔＪ遺伝子及びＬ−メチオニンの製造法
JP2003180348A (ja) 1999-07-02 2003-07-02 Ajinomoto Co Inc Ｌ−アミノ酸の製造法
DE19959650A1 (de) 1999-12-10 2001-06-13 Degussa Neue für die Gene sdhA, sdhB und sdhC codierende Nukleotidsequenzen
ATE434663T1 (de) * 2001-07-18 2009-07-15 Evonik Degussa Gmbh Verfahren zur herstellung von l-threonin durch enterobakteriaceae-stämmen mit verstärkter exprimierung der succ und sucd gene
US20060270013A1 (en) 2003-02-18 2006-11-30 Michel Chateau Method for the production of evolved microorganisms which permit the generation or modification of metabolic pathways
WO2005007862A2 (en) 2003-07-08 2005-01-27 Novus Internation, Inc Methionine recovery processes
DE10359668A1 (de) 2003-12-18 2005-07-14 Basf Ag Verfahren zur Herstellung von Methionin
WO2005111202A1 (en) 2004-05-12 2005-11-24 Metabolic Explorer Recombinant enzyme with altered feedback sensitivity
RU2413001C2 (ru) 2005-07-18 2011-02-27 Эвоник Дегусса Гмбх Применение диметилдисульфида для продукции метионина микроорганизмами
WO2007017710A1 (en) 2005-08-11 2007-02-15 Metabolic Explorer Process for the preparation of aspartate and derived amino acids like lysine, threonine, isoleucine, methionine, homoserine, or valine employing a microorganism with enhanced isocitrate lyase and/or malate synthase expression
WO2007020295A2 (en) 2005-08-18 2007-02-22 Basf Ag Microorganisms with increased efficiency for methionine synthesis
JP2007185184A (ja) 2005-12-16 2007-07-26 Ajinomoto Co Inc Ｌ−アミノ酸生産菌及びｌ−アミノ酸の製造法
EP2314710B1 (en) 2006-01-04 2016-03-30 Metabolic Explorer Method for the production of methionine by culturing a microorganism modified to enhance production of cysteine
WO2009043372A1 (en) 2007-10-02 2009-04-09 Metabolic Explorer Increasing methionine yield
WO2009078973A2 (en) 2007-12-13 2009-06-25 Glycos Biotechnologies, Incorporated Microbial conversion of oils and fatty acids to high-value chemicals
CN102159720A (zh) 2008-04-30 2011-08-17 赢创德固赛有限公司 使用具有降低的异柠檬酸脱氢酶活性的微生物生产甲硫氨酸的方法

Also Published As

Publication number Publication date
US9267160B2 (en) 2016-02-23
PL2519643T3 (pl) 2018-03-30
ES2654659T3 (es) 2018-02-14
BR112012016274A8 (pt) 2017-09-19
JP5819316B2 (ja) 2015-11-24
KR20120106999A (ko) 2012-09-27
CN102712941A (zh) 2012-10-03
WO2011080542A1 (en) 2011-07-07
AR079764A1 (es) 2012-02-15
EP2519643A1 (en) 2012-11-07
KR101770123B1 (ko) 2017-08-23
BR112012016274B1 (pt) 2019-08-20
JP2013516167A (ja) 2013-05-13
EP2519643B1 (en) 2017-11-15
US20120288903A1 (en) 2012-11-15
BR112012016274A2 (pt) 2015-10-06

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