WO2006014028A1 - Extrait de pédoncule de pomme de terre et utilisation - Google Patents

Extrait de pédoncule de pomme de terre et utilisation Download PDF

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Publication number
WO2006014028A1
WO2006014028A1 PCT/JP2005/014799 JP2005014799W WO2006014028A1 WO 2006014028 A1 WO2006014028 A1 WO 2006014028A1 JP 2005014799 W JP2005014799 W JP 2005014799W WO 2006014028 A1 WO2006014028 A1 WO 2006014028A1
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Prior art keywords
sweet potato
extract
water
polyphenol
soluble
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PCT/JP2005/014799
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English (en)
Japanese (ja)
Inventor
Satoru Suzuki
Shigekazu Kitani
Izumi Yasutani
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Mochida Pharmaceutical Co., Ltd.
Toyota Jidosha Kabushiki Kaisha
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Application filed by Mochida Pharmaceutical Co., Ltd., Toyota Jidosha Kabushiki Kaisha filed Critical Mochida Pharmaceutical Co., Ltd.
Priority to JP2006531750A priority Critical patent/JPWO2006014028A1/ja
Publication of WO2006014028A1 publication Critical patent/WO2006014028A1/fr

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/39—Convolvulaceae (Morning-glory family), e.g. bindweed
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
    • A23L19/10—Products from fruits or vegetables; Preparation or treatment thereof of tuberous or like starch containing root crops
    • A23L19/105—Sweet potatoes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7042—Compounds having saccharide radicals and heterocyclic rings
    • A61K31/7048—Compounds having saccharide radicals and heterocyclic rings having oxygen as a ring hetero atom, e.g. leucoglucosan, hesperidin, erythromycin, nystatin, digitoxin or digoxin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/16—Emollients or protectives, e.g. against radiation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/24—Antidepressants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/04—Anorexiants; Antiobesity agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/06—Antihyperlipidemics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/08—Antiallergic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00—General protective or antinoxious agents
    • A61P39/06—Free radical scavengers or antioxidants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Definitions

  • the present invention relates to a water-soluble sweetpotato extract containing polyphenol and a method for producing the same.
  • the present invention also provides a functional food and drink, a functional material, a pharmaceutical, an antioxidant, a hepatoprotectant, a tyrosinase inhibitor, a sugar absorption inhibitor, and a neutral fat absorption inhibitor, comprising the sweet potato foliage extract as an active ingredient. It also relates to obesity prevention / treatment agents, antidepressants, anti-fatigue agents, deodorants, oils and fats and foods containing fats. Further, the present invention relates to a food or drink containing the sweet potato stem and leaf extract. Background art
  • Polyphenolic compounds are widely contained in plants as secondary metabolites of plants, and their types are known to be diverse. These polyphenol compounds have long been studied in the field of pharmacology because of their various physiological activities, and in recent years they have attracted attention in the field of food chemistry.
  • the flavonoids of Chinese sweet potato leaves are quercetin 1- o- 1 D-darcopyranosyl 1 (6 ⁇ 1) — o— ⁇ — L-humnohifunoside quercetin-3-0— 1 D— glucopyranosyl—, 6 ⁇ 1)- 0- a -L-rhamnopyranoside), Kaempfellore 1, _Nmenazulee 1 Tesole (kaempf erol-4, 7-diraethyl ether), Gesolecetin 3 1 o 1 j3 1 D-gnoreside (quercetin-3- 0- ] 3-D-glucoside and quercetin have been reported by HPLC (Journal of Instrumental Analysis (1996), 15 (1), 71-74) On the other hand, 60 kinds of sweet potatoes were freeze-dried.
  • Polyphenol component was identified by HPLC analysis of powdered methanol extract, and it was reported that the main component was force phenolic acid and its five derivatives, and the same component was detected in all varieties (Journal of Agricultural and Food Chemistry (2002), 50, 3718-3722).
  • the ethanol extract above the sweet potato has amylase inhibitory activity, a darcosidase inhibitory activity, and ACE inhibitory activity. (Japanese Patent Laid-Open No. 2000-0 7 5 6 3 8). Disclosure of the invention
  • Polyphenols contained in plants are known to have a wide range of physiological effects, extracted from plants such as tea, and applied to functional foods.
  • concentration to demonstrate its functionality is important.
  • the taste of polyphenols can be problematic and difficult to use in foods.
  • Polyphenols are known to have a strong bitter taste, like tea power, and they are required to exhibit functionality even at low concentrations.
  • extracts containing polyphenols such as yew leaf extract and soybean isoflavone may be poorly soluble in water, but those with good water solubility are required for addition to beverages and the like.
  • the present inventors screened various raw materials, studied a method for producing a polyphenol-containing extract, and obtained such a method.
  • the physiological effects of the obtained polyphenol-containing extracts were examined.
  • sweet potato stems and leaves are used as raw materials, extracted with a water-containing organic solvent to obtain water-soluble components, and the polyphenol fraction is purified to refine the polyphenol fraction. It was found that the extract can be obtained efficiently. Further, when the physiological action of the extract was examined, it was found that the extract has a higher activity than expected, and the present invention has been achieved.
  • the present invention provides a water-soluble sweet potato stem-and-leaf extract containing polyphenol as shown below, a method for producing the same, a use of the sweet potato stem-and-leaf extract, and the like.
  • the content of quercetin in the polyphenol is 3-darcoside.
  • the sweet potato according to any one of the above (1) or (4) characterized in that it contains at least quercetin 1-darcoside and 3,5-dicaffeoylquinic acid as components of polyphenol. Forage extract.
  • a sweet potato foliage extract derived from sweet potato foliage characterized by containing at least quercetin_3-darcoside and 3,5-dicaf euinorequinic acid as polyphenol components.
  • a water-soluble extract derived from sweet potato stems and leaves which contains at least quercetin-3-darcoside, chlorogenic acid and 3,5-dicaffeoylquinic acid as polyphenol components.
  • a step of extracting sweet potato stems and leaves with a water-containing organic solvent, and a step of removing the organic solvent from the extract obtained by the extraction step to obtain a water fraction, comprising a polyphenol-containing aqueous solution A method for producing a natural sweet potato stem and leaf extract.
  • a water-soluble sweet potato stem-and-leaf extract characterized by containing at least quercetin-3-dalcoside.
  • a water-soluble sweet potato stem-and-leaf extract obtained through a process of water-extracting sweet potato foliage, having a polyphenol content of 10% or more, and at least quercetin as a component of the polyphenol.
  • a water-soluble sweet potato stem and leaf extract characterized by containing darcoside.
  • the polyphenol containing water-soluble sweet potato stem and leaf extract which has various beneficial activity for a human body can be provided.
  • the extract of the present invention is water-soluble Therefore, for example, there is an advantage that it can be taken in various beverages.
  • a polyphenol-containing extract can be efficiently obtained without using any special materials or facilities.
  • the sweet potato foliage extract of the present invention has such activities as antioxidative activity, tyrosinase inhibitory activity, hepatoprotective activity, sugar and neutral fat absorption inhibitory activity, and so on. It is useful as a functional food / beverage product, functional material, medicine, etc. Brief Description of Drawings
  • Fig. 1 shows the liquid chromatography matrix of sweet potato stem and leaf extract obtained from sweet potato stems and leaves.
  • Figure 2 is a liquid chromatographic chart of sweet potato stem and leaf extract obtained from sweet potato leaves.
  • FIG. 3 is a graph showing the antioxidant effect of the extract of sweet potato foliage in the rat liver injury model of Example 9 administered with APPH.
  • A shows the measurement result of urinary 8-OHdG
  • B A R S shows the measurement result of thiobarbituric acid reactant
  • FIG. 4 is a graph showing the glucose absorption suppression effect of sweet potato stem and leaf extract in the rat glucose tolerance test of Example 11.
  • FIG. 5 is a graph showing the results of a comparative test of guava leaf extract on the blood glucose elevation inhibitory effect of Example 11.
  • FIG. 6 is a graph showing the neutral fat absorption inhibitory action of the sweet potato shoot extract in the rat fat tolerance test of Example 12.
  • FIG. 7 is a graph showing the results of a comparative test with the green tea extract on the fat absorption inhibitory effect of Example 12;
  • FIG. 8 is a graph showing the deodorizing effect of the sweet potato shoot extract of Example 16. Results for ammonia (a), mercabtan (b), and trimethylamine (c) Represent each one.
  • FIG. 9 shows the state of adipocytes in Example 17.
  • FIG. 10 is a graph showing the average time of EDB and IM0 of mice in the forced swimming test of Example 18.
  • FIG. 11 is a graph showing the deterioration time of fats and oils at the concentration of the sweet potato stem and leaf extract of Example 19; BEST MODE FOR CARRYING OUT THE INVENTION
  • the first aspect of the present invention relates to a sweet potato foliage extract, which is a water-soluble extract derived from sweet potato foliage and contains polyphenol alcohol.
  • “Satsumaimo” refers to plants belonging to the genus Sapamo, which is a convolvulaceae family, and any cultivar of this genus can be used, for example, Shirouta power, Koganesengan, SU KU H , Tanegashima purple, Shimonimo, and the like, preferably the sweet potato variety belonging to white sweet potato.
  • White sweet potato is a sweet potato that has a tuberous root that contains almost no anthocyanin and is white. More preferred is Shiroyutaka.
  • sweet potato foliage is used to mean a material containing at least leaves, preferably both foliage, of the ground portion of sweet potato. Especially preferred are leaves only. Since sweet potato foliage is usually discarded for no use, the use of such materials is also significant in terms of resource reuse.
  • the sweet potato foliage used here may be a fresh material or a dry material. A dry material is preferred, and a suitable method such as sun drying, hot air drying, freeze drying and the like can be selected as the drying method, but sun drying is preferred.
  • sweet potato stems and leaves can be crushed in consideration of extraction efficiency. It is also possible to increase the taste and flavor of the extract by appropriately roasting sweet potato stems and leaves.
  • the sweet potato stem and leaf extract of the present invention is water-soluble, for example, it can be taken in a variety of beverages.
  • water-soluble means that it can be dissolved in at least lOOmg or more, preferably 1 g or more, more preferably 10 g or more, and particularly preferably 50 g or more in 1 L of water at room temperature.
  • the sweet potato foliage extract of the present invention contains polyphenol derived from sweet potato foliage as an active ingredient.
  • the sweet potato stem and leaf extract of the present invention is preferably 10% or more, preferably 15% or more, more preferably 20% or more, more preferably 25% or more, and particularly preferably 30% or more of the total nonvolatile component weight in terms of catechin.
  • the upper limit of the polyphenol content is not particularly limited, but the sweetpotato shoot extract of the present invention usually contains up to about 60% polyphenol. Polyphenol content may be expressed in terms of gallic acid.
  • polyphenol components derived from the stems and leaves of sweet potato include quercetin glycosides such as quercetin 1-darcoside and quercetin 1-galactosid, chlorogenic acid, 3, 4-dicaffeoylquinic acid, 3, 5-di Examples include caffeic acid derivatives such as caffeoylquinic acid, 4,5-dicaffeoylquinic acid, and 3,4,5-tricaffeoylquinic acid.
  • the polyphenol derived from the sweet potato foliage may contain an unidentified polyphenol component, but the present invention does not exclude such an unidentified polyphenol component.
  • quercetin 1-darcoside is a preferred polyphenol component because of its good absorption into the body (Olthof MR.
  • the water-soluble sweet potato extract of the present invention has at least quercetin-3-darcoside as a constituent component of the polyphenol component in the water-soluble sweet potato shoot extract. Yes, and more preferably, one containing quercetin 1-darcoside and 3,5-dicuff oil quinic acid.
  • quercetin-3-darcoside, chlorogenic acid and 3,5-dicafluoroquinic acid More preferably, at least quercetin-3-darcoside, chlorogenic acid and 3,5-dicafluoroquinic acid, particularly preferably at least quercetin-1-darcoside, quercetin-3 galactosid, chlorogenic acid and 3,5-dioxy It is desirable to contain caffeoylquinic acid.
  • the content of quercetin 1-3-darcoside in the polyphenol is 1% or more, preferably 2% or more, more preferably in the sweet potato extract. Is preferably 3% or more, particularly preferably 5% or more.
  • the upper limit of the content of quercetin-3-darcoside is not limited, but the sweet potato shoot extract of the present invention usually contains up to about 30% quercetin-1-darcoside in the polyphenol.
  • the leaves of sweet potato are rich in quercetin glycosides such as quercetin 1-3-darcoside and monogalactosid. You can get minutes.
  • Quercetin 1-3-galactosid has never been known as a component of sweet potato foliage, and is one of the components that characterize the sweet potato foliage extract of the present invention.
  • the content of 3,5-dicaffeoylquinic acid in the polyphenol is preferably 1% or more, more preferably 2% or more, and particularly preferably 3% or more. It is desirable that it also contains chlorogenic acid.
  • the sweet potato stem and leaf extract of the present invention includes, for example, amino acids such as phenylalanine and triftophan in addition to polyphenol.
  • Subcomponents such as organic acids, polysaccharides and glycolipids may be included.
  • organic acid include quinic acid
  • examples of the polysaccharide include oligosaccharides
  • examples of the glycolipid include charapine
  • other components other than these polyphenols are also preferable for the sweet potato stem and leaf extract of the present invention. It is a component.
  • These polyphenol components or subcomponents can be quantified by, for example, high performance liquid chromatography (HPLC).
  • the production method of the sweet potato foliage extract of the present invention is not particularly limited. From the viewpoint of extraction efficiency, extraction with a water-containing organic solvent described later is preferable, but it can also be obtained by water extraction.
  • Hot water, hot water or cold water can be used for water extraction, and the target components are extracted by placing the foliage in water at the target temperature and immersing it for a certain period of time.
  • the foliage is preferably dried and crushed beforehand.
  • As the extraction temperature for example, in the case of hot water, 60 to 100 ° C, preferably 60 to 80 ° C, in the case of hot water, 30 to 60 ° C, preferably 50 to 60 ° C. In the case of C and cold water, it is 0 to 30 ° C., preferably 10 to 30 ° C.
  • hot water extraction is preferable from the viewpoint of extraction efficiency.
  • the extraction time is not particularly limited, but in the case of hot water, it is 10 to 150 minutes, preferably 20 minutes to 70 minutes, and it is preferable to extract over a long time as the extraction temperature is lowered, In the case of cold water extraction, it can be extracted over a day. Also, when extracting, pressurize Extraction is possible, especially when the extraction temperature is to be lowered. After extraction, the extract can be cooled to remove the precipitate, and a sweet potato stem and leaf extract can be obtained. For drip extraction, place the foliage on a cloth or paper filter, pour hot water from above, and filter. It can be selected according to the desired taste, flavor, and aroma.
  • a purification process described later may be added, but before performing purification using a column, a step of diluting the extract, or a precipitation removal accelerator (organic acid, ethanol, etc.) is added when removing the precipitate. It is desirable to add a process to facilitate column purification such as the process of The obtained aqueous solution of the sweet potato foliage extract may be further concentrated and used, or may be dried to remove water to obtain a powdered sweet potato foliage extract.
  • the second aspect of the present invention includes a step of extracting sweet potato stems and leaves with a water-containing organic solvent, a step of removing the organic solvent from the extract obtained by the extraction step and obtaining a water fraction, and polyphenol from the water fraction.
  • the present invention relates to a method for producing a polyphenol-containing sweet potato foliage extract comprising a purification step.
  • the third aspect of the present invention relates to a sweet potato foliage extract obtainable by this production method.
  • sweet potato foliage is subjected to extraction using a water-containing organic solvent.
  • the sweet potato foliage used here may be a fresh material or a dry material, and is preferably pulverized in consideration of extraction efficiency.
  • the extraction method used here is not particularly limited, and known methods such as cold immersion, digestion, or refluxing can be used under standing or stirring conditions.
  • a water-containing organic solvent is added to the dried potato roots and leaves, mixed, and extracted while immersed or heated under reflux.
  • the amount of water-containing organic solvent to be added is not particularly limited.
  • When using a dry material use at least 3 times the volume of the material and use fresh material. In this case, it is desirable to add a water-containing organic solvent having a volume of at least 5 times the volume of the material.
  • the water-containing organic solvent used for the extraction is not particularly limited as long as it can extract polyphenols from sweet potato stalks and leaves.
  • a mixed solution of water and a hydrophilic organic solvent is used.
  • the organic solvent content in the mixed solution is, for example, 40 to 90% by volume, preferably 40 to 80% by volume, and more preferably 50 to 80% by volume.
  • hydrophilic organic solvents examples include methanol, ethanol, propanol, isopropanol, ptanol, isobutanol, t-butanol and other monovalent lower vinyl alcohols; ethylene glycolate, diethylene glycolate, triethylene glycol, propylene Polyhydric alcohols such as glycol and dipropylene glycol; Cellosonolevs such as methinoreserosonoleb and etherosolozoleb; Ethers such as dioxane and tetrahydrofuran; Ketones such as acetone and methylethylketone.
  • hydrous alcohols ethanol, methanol, etc.
  • hydrous alcohols can be particularly preferably used.
  • the inventors of the present invention have found that the extracted polyphenols decompose and disappear with time when the water content of the hydrous alcohol that is the extract at the time of extraction is high.
  • the alcohol concentration was too high, the yield decreased and the odor and taste tended to deteriorate, and it was found that there was an appropriate alcohol concentration.
  • the water alcohol used in the extraction process of the present invention e.g., ethanol
  • the water alcohol used in the extraction process of the present invention is preferably 40 to 90 volume%, more preferably, 50 to 80 volume 0/0 alcohol (e.g., ethanol). In is there. In particular, 60% by volume or more of ethanol is desirable.
  • the extraction as described above is carried out, for example, at 10 to 70 ° C., preferably 40 to 60 ° C.
  • the organic solvent is removed from the extract obtained as described above to obtain a water fraction.
  • Methods for removing the organic solvent include those used by those skilled in the art depending on the type of hydrous organic solvent used, such as evaporation of the organic solvent by concentration under reduced pressure, fractionation of the organic solvent with a non-hydrophilic organic solvent (eg, hexane).
  • the method to obtain can be selected suitably.
  • hydrous ethanol it is preferable to distill off the alcohol by concentration under reduced pressure.
  • the removal of the organic solvent is preferably carried out until the ratio of water in the solution is at least 70% or more.
  • the extract obtained by removing the organic solvent thus obtained is referred to as a water fraction.
  • water-insoluble or sparingly soluble components are precipitated or fractionated, so that the water fraction is in a state where the water-insoluble sweet potato foliage components are removed. Therefore, it is considered that this process contributes to increase the water solubility of the sweet potato foliage extract of the present invention.
  • the water fraction may be allowed to stand still, or an operation of adding several times the amount of water may be performed.
  • Components that are insoluble or hardly soluble in the precipitated water can be removed by an appropriate means such as removal with decant, fractionation with a non-hydrophilic organic solvent (eg hexane, etc.), filtration.
  • purifying polyphenol means increasing the proportion of polyphenol in the total content of non-volatile components in the sample. Specifically, purification can be achieved by removing various non-volatile components other than polyphenol from the water fraction. In the finally obtained sweetpotato leaf extract (usually in the form of a powder from which volatile components such as water have been removed), the content of polyphenol in the total nonvolatile components is 10% or more, preferably 20 If it exceeds%, the polyphenol fraction has been purified.
  • a known method such as a method using an adsorbent is used.
  • adsorbent capable of selectively adsorbing and eluting polyphenols
  • the polyphenol is adsorbed on the adsorbent, and after washing, purified polyphenol is eluted with the eluent.
  • a hydrophilic organic solvent such as ethanol may be added to the water fraction to precipitate and remove non-volatile components (such as polysaccharides) that are hardly soluble in the organic solvent.
  • a combination of such precipitation removal method and column purification may be used.
  • adsorbent used here for example, a synthetic adsorption resin of a crosslinked styrene-based porous polymer, an anion exchange resin, octadecyl group chemically bonded silica gel (0DS), or the like can be used.
  • Commercially available synthetic resin adsorbents include, for example, Mitsubishi Kasei's Diaion HP—20, HP—21, HP 2 MG, SP 2 07, SP 8 25, SP 8 5 0; And 'Haas Amberlite X AD—2, X AD-4, X AD-7, X AD-2 26, etc. can be used. Conditions for carrying out the purification method using the adsorbent can be easily set by those skilled in the art. If a manual is attached to a commercially available adsorbent, the adsorption operation can be performed according to the instructions in the manual.
  • the impurities are usually washed and removed by passing a washing solution through the column.
  • the cleaning liquid is water (preferably distilled water), 1 to 10 weights.
  • a / 0 ethanol aqueous solution is preferably used. Normally, it is recommended to pass a cleaning solution of about 1 to 10 times the amount of resin.
  • the polyphenol-containing fraction can be eluted and collected by passing the eluate through a column.
  • the eluent for example, a water-containing organic solvent such as water-containing alcohol, water-containing acetone, or water-containing acetonitrile can be used.
  • a particularly suitable eluent is 20% or more ethanol aqueous solution or 100% ethanol.
  • the flow rate of the eluate is preferably about 2 to 6 times the amount of resin.
  • the organic solvent can be distilled off to obtain an aqueous solution of sweet potato stem and leaf extract. Further, the water content of the aqueous solution of the sweet potato foliage extract can be removed by spray drying or freeze drying to obtain a powdered sweet potato foliage extract. If necessary, the powder can be made to have a uniform particle size using a powder jar, or a fine powder.
  • powder auxiliary agents such as dextrin can be added for spray drying or freeze drying, and stabilizers, excipients and the like can be added to the obtained powder.
  • composition thus obtained that is, a liquid preparation or a powder preparation, contains a sweet potato foliage extract-containing functional food and drink, a functional material, a 'pharmaceutical preparation, an antioxidant, a hepatoprotectant, tyrosinase It can be used as an inhibitor, sugar absorption inhibitor, neutral fat absorption inhibitor, obesity prevention * therapeutic agent, antidepressant, anti-fatigue agent, deodorant, fat and oil and fat-containing food deterioration preventive agent, etc.
  • the third aspect of the present invention is a sweet potato leaf extract obtained by the above production method. If the product is the same as the sweet potato foliage extract obtained by the above production method, the product obtained by a different production method is included in the sweet potato foliage extract of the present invention. Whether the product is the same is confirmed by, for example, checking whether it has the characteristics described in the first aspect of the present invention. be able to. That is, a preferred form of the sweet potato stalk-and-leaves extract obtainable by the above production method is obtained by the above production method and is water-soluble, polyphenol content, polyphenol described in the first aspect of the present invention. It is a sweet potato stem and leaf extract characterized by the content of various components in the component and polyphenol. (Use)
  • a pharmaceutical, a functional food / beverage product, a functional material, an antioxidant comprising the sweet potato foliage extract described in the first aspect or the third aspect of the present invention as an active ingredient, Hepatoprotectant, tyrosinase inhibitor, sugar absorption inhibitor, neutral fat absorption inhibitor, obesity prevention / treatment agent, metabolic syndrome prevention / treatment agent, antidepressant, anti-fatigue agent, deodorant, oil and fat It relates to a deterioration preventive agent for contained foods. Furthermore, the present invention also relates to a food or drink containing the sweet potato stem and leaf extract.
  • the production method of the sweet potato stem and leaf extract used here is preferably the above production method, but is not particularly limited to this, and any known method can be used.
  • a preferred embodiment of the sweet potato foliage extract used here is a sweet potato foliage extract having the characteristics described in the first aspect or the third aspect of this effort.
  • the antioxidant ability of the sweet potato stem and leaf extract of the present invention has been confirmed by various antioxidant ability experiments in Example 8 described later. Therefore, the sweet potato foliage extract of the present invention can be used as an active ingredient of an antioxidant.
  • the antioxidant of the present invention has been confirmed by an experiment using the rat of Example 9 to have an action of eliminating active oxygen even in a living body. Therefore, the antioxidant of the present invention can be used for the prevention or treatment of various diseases and symptoms involving active oxygen. Examples of diseases involving active oxygen include cancers that can be induced by active oxygen, allergies, diseases involving lipid peroxides such as arteriosclerosis, and the like. Symptoms involving active oxygen include various symptoms associated with aging, spots, wrinkles, and rough skin. 2. Hepatoprotectant
  • the sweet potato foliage extract of the present invention can be used as an active ingredient of a hepatoprotectant.
  • the hepatoprotective agent of the present invention can be used for prevention or treatment and amelioration of diseases and symptoms associated with liver function failure or reduction.
  • diseases and symptoms associated with liver function failure or reduction include liver diseases such as cirrhosis, viral hepatitis, fulminant hepatitis, liver cancer, liver damage and exhaustion.
  • Symptoms involving failure or decline in liver function include hangover, alcoholic hepatitis, malaise, fatigue, and poor circulation. In order to prevent these diseases and symptoms, it is preferable to improve the liver function by ingesting the hepatoprotective agent of the present invention in advance or during normal times.
  • the sweet potato shoot extract of the present invention has tyrosinase inhibitory activity.
  • Tyrosinase also called polyphenoloxidase, is an enzyme that inactivates polyphenol and exacerbates the production of melanin in the skin and the browning reaction of the product. Therefore, the sweet potato shoot extract of the present invention can be used as an active ingredient of a tyrosinase inhibitor.
  • the tyrosinase inhibitor of the present invention can be used for the prevention or treatment and improvement of diseases and symptoms associated with tyrosinase. Symptoms involving such tyrosinase include, for example, skin melanin development, sunburn, and pigmentation. In addition, by taking the tyrosinase inhibitor of the present invention, the occurrence of melanin in the skin can be suppressed, and a whitening effect can be expected. Therefore, the sweet potato foliage extract of the present invention can also be used as an active ingredient in cosmetics or functional foods having a whitening effect.
  • sugar absorption inhibitor It has been confirmed by experiments using rats described in Example 11 described later that the sweet potato shoot extract of the present invention has a sugar absorption inhibitory action. Therefore, the sweet potato foliage extract of the present invention can be used as an active ingredient of a sugar absorption inhibitor.
  • the sugar absorption inhibitor of the present invention has an action of suppressing sugar absorption and suppressing an increase in blood glucose level, and is therefore used for prevention or treatment and improvement of diseases and symptoms related to sugar ingested in the human body. be able to. Examples of such diseases involving sugar include diabetes, obesity, and diabetic complications.
  • the potato leaf extract of the present invention has an effect of preventing hyperglycemia after meals, and therefore can be a functional food material appealing to those who are concerned about blood sugar levels.
  • the sweet potato shoot extract of the present invention has a neutral fat absorption inhibitory action and an absorption delay action. Therefore, the sweet potato foliage extract of the present invention can be used as an active ingredient of a neutral fat absorption inhibitor.
  • the neutral fat absorption inhibitor of the present invention Since the neutral fat absorption inhibitor of the present invention has an action of suppressing neutral fat absorption, it can be used for the prevention or treatment and improvement of diseases and symptoms associated with an excess of neutral fat. Examples of such diseases include hypercholesterolemia, hyperlipidemia, ischemic heart disease, and cerebrovascular disorder.
  • the sweet potato extract of the present invention can be a functional food material that appeals to people who are concerned about body fat.
  • the sweet potato foliage extract of the present invention has an adipocyte differentiation and hypertrophy inhibitory effect by the in vitro rat mesentery-derived white adipocyte differentiation and hypertrophy suppression test described in Example 17. It has been confirmed. Therefore, the sweet potato foliage extract of the present invention can be used as an active ingredient of an inhibitor of adipocyte differentiation and hypertrophy, and therefore can be used as a prophylactic / therapeutic agent for obesity. Furthermore, the potato shoot extract has the above-mentioned sugar absorption inhibitory action and neutral fat absorption inhibitory action. Therefore, the synergistic effect of these actions can be expected to prevent and treat obesity.
  • the prophylactic / therapeutic agent for obesity according to the present invention can be used for the prevention or treatment and amelioration of diseases and symptoms associated with obesity. Examples of such diseases include arteriosclerosis, hypertension, and diabetes. Moreover, the sweet potato foliage extract of the present invention can be a functional food material that appeals to people who are concerned about fattening and body fat.
  • the sweet potato stem and leaf extract of the present invention prevents and ameliorates hyperglycemic symptoms by inhibiting glucose absorption, and prevents and improves hyperlipidemia, especially hypertriglyceridemia, by inhibiting triglyceride absorption. It can also be used as an active ingredient of a preventive / therapeutic agent for metabolic syndrome in addition to having an effect of preventing / treating obesity by inhibiting fat cell differentiation and hypertrophy.
  • Metabolic syndrome is mainly caused by the accumulation of visceral fat, and multiple risk factors such as obesity, diabetes, hyperlipidemia, and hypertension accumulate, and atherosclerosis and coronary artery disease (Infarct or cerebral infarction) A disease state with an increased risk of onset.
  • the sweet potato stalk-and-leaves extract of the present invention has a plurality of actions effective for preventing and improving metabolic syndrome, and therefore can be a functional food material appealing to those who are concerned about metabolic syndrome.
  • sweet potato stem and leaf extract of the present invention has an antidepressant action has been confirmed by the examination of the antidepressant action by the mouse forced swimming method described in Example 18. Therefore, the sweet potato stem and leaf extract of the present invention can be used as an active ingredient of an antidepressant. It also reduces stress and can be expected to relax.
  • the antidepressant of the present invention has an antidepressant action, it can be used for the prevention or treatment and amelioration of depression and symptoms associated with depression.
  • the potato stem and leaf extract of the present invention can be a functional food material that appeals to people who are concerned about depression and modern people who are stressful. 9 Anti-fatigue agent
  • the sweet potato foliage extract of the present invention can be used as an active ingredient of an anti-fatigue agent. For example, it reduces physical fatigue caused by prolonged exercise, and relaxes the mood to improve mental stress and fatigue. It is expected to prevent, reduce, and improve physical and mental fatigue.
  • the sweet potato foliage extract of the present invention can be a functional food material that is appealing to athletes who are constantly subject to physical fatigue, and those who are likely to feel physical and mental fatigue due to long working hours.
  • the sweet potato stem and leaf extract of the present invention has a deodorizing effect on causative substances such as the bad breath, body odor, and fecal odor of human pets. Therefore, the sweet potato stem and leaf extract of the present invention can be used as an active ingredient of a deodorant.
  • the sweet potato stem and leaf extract can be used for the deodorization of human pet's halitosis, body odor and stool odor, and the deodorization of beverages and foods. It can be used in a very wide range of applications among deodorizing substances. Can be a new material.
  • the sweet potato stalk and leaf extract of the present invention is particularly useful as an anti-degradation agent for animal fats and oils, as an anti-degradation agent that suppresses deterioration of fats and oils.
  • Processed foods that use animal fats such as lard and lard (fried potatoes, snacks, cups, etc.) and other various fats and oils can be added to prevent the deterioration of fats and oils.
  • powdery sweet potato stem and leaf extracts can be added to the fats and oils.
  • sweet potato stem and leaf extract mixed with oil.
  • powdered sweet potato foliage extract to fried clothes such as toppings, bread crumbs, and wheat flour, and mixing it with food ingredients processed with fats and oils, the food is cooked with fats and oils and at the same time Deterioration can also be prevented.
  • the amount added to the food fat is not particularly limited, but is preferably 10 ppm or more, more preferably 200 ppm or more, particularly preferably 500 ppm or more, and the upper limit is not limited, but the sweet potato stem and leaf extract is processed into soft capsules, 50 weight y. It is also possible to add a degree of sweet potato foliage extract. 1 2.
  • the sweet potato stem and leaf extract of the present invention has activities or actions such as antioxidant activity, hepatoprotective action, tyrosinase inhibitory action, sugar and neutral fat absorption inhibitory action, etc., and has an excellent function as a functional material. . Therefore, the sweet potato foliage extract of the present invention can be used as an active ingredient of a functional material.
  • Functional materials can be used for various purposes as functional materials and raw materials.
  • the form is not particularly limited, and the active ingredient may be only sweet potato stem and leaf extract, or may be in a state mixed with other functional materials and raw materials, and can be in any form such as powder, liquid, and molded product. It can take.
  • Example 20 it was confirmed that granules, tablets, and gummi were produced from sweet potato stem and leaf extract as raw materials, and were easily used as functional materials.
  • Daily intake is 0.1 mg / kg or more per adult, preferably 1 mg / kg or more, more preferably 1 O mg / kg or more, particularly preferably 3 O mg / kg or more.
  • the upper limit of daily intake is not particularly limited, but for example, it is within the allowable range up to about 2000 mg Zkg. However, the range of preferred intake may naturally vary depending on the product form and individual characteristics.
  • Functional materials can be used for food antioxidants, food dye stabilizers, deodorants, etc. because they have an antioxidant effect for purposes other than ingestion as food.
  • it since it has tyrosinase inhibitory activity, it can be used as a cosmetic. It is done.
  • Cosmetics can be produced according to conventional methods. Cosmetics are not limited to general skin cosmetics, but include quasi-drugs, designated quasi-drugs, topical drugs, etc., and the dosage form is arbitrarily selected according to the purpose. be able to. That is, it can be in the form of creams, ointments, emulsions, solutions, gels and the like, and forms such as packs, lotions, powders and sticks.
  • the blending amount of the extract is preferably 0.01 to 1.0%, particularly preferably 0.01 to 0.1% in terms of the solid content of the component, based on the total amount of the cosmetic. .
  • the functional food or drink comprising the sweet potato foliage extract of the present invention as an active ingredient is provided by formulating the sweet potato foliage extract of the present invention into an appropriate formulation or adding it to the food or drink.
  • the pharmaceutical comprising the sweet potato stem and leaf extract of the present invention as an active ingredient
  • sweet potato foliage extract of the present invention is provided by formulating the sweet potato foliage extract of the present invention into an appropriate formulation.
  • oral forms such as powders, granules, capsules, pills, tablets and other solid preparations, liquids such as liquids, suspensions and emulsions, etc.
  • An administration agent is mentioned.
  • This orally administered agent is composed of excipients, disintegrants, binders, lubricants, surfactants, alcohols, water generally used depending on the form of the orally administered agent.
  • Sweet potato foliage extract varies depending on use and form in the oral dosage form.
  • the sweet potato foliage extract of the present invention when administered as a pharmaceutical, the dosage varies depending on its action, target disease, administration subject, administration route, etc. For example, in an adult generally weighing 60 kg The polyphenol content is about 1 mg to 2000 mg, preferably about 10 mg to 500 mg, more preferably 30 mg to 300 mg per day.
  • the sweet potato foliage extract of the present invention has characteristics that can suppress excessive absorption of carbohydrates and neutral fat in the diet and production of lipid peroxide in the liver, and is also promising for a forceful diet.
  • Sugar-rich products fruit juice, juice, beer, liquor, chocolate, cookie, honey products, strawberry, gum, jelly, eye cream Processed foods using raw materials (fish oil, margarine, dairy products, butter, egg yolk oil, mayonnaise, salad oil, dressing) that contain a lot of neutral fat.
  • Deep-fried food products (tempura, potato chips and other snacks, fried rice crackers, instant rice cakes), and other ingredients that promote reduced absorption of sugar and neutral fat and body fat (green tea, mulberry leaf tea, guava, tomorrow, It is effective when mixed with products for functional enhancement using soy protein / soy milk, chitin / chitosan, alginic acid, oligosaccharide “dextrin” dietary fiber).
  • corn isomerized linoleic acid, sesamin, vitamin E, CoQ10, c lipoic acid, carnitine, amino acid (valine, leucine, isoleucine, arginine, theanine, histidine), ginseng (Ezoukogi, red ginseng, western ginseng) , Organic acids (Vitamin c, Vinegar, Quenic acid), Vitamin B group, Sutsubon, Ants, Bracenta, Pepper, Black pepper, Carotenoids (Lycopene, Fastaxanthin, Rutin), Isoflavone, Mushroom, Seaweed Nutritional supplements that combine substances selected from sugars (agaricus, fucoidan), various herbs (yew leaves, pine bark, grape extract), berries (blueberries), and lipids (EPA, DHA) are also effective.
  • the amount added to foods and beverages is preferably such that the daily intake of sweet potato stem and leaf extract is 1 mg / kg or more, preferably 3 Omg / kg or more per day for an adult.
  • the upper limit of daily intake is not particularly limited, but for example, it is within the allowable range up to about 2000 mg / kg. However, the preferred intake range may naturally vary depending on the product form and individual characteristics.
  • the water-soluble sweet potato shoot extract of the present invention has a polyphenol content of 0.00 l mg / ml to 30 mg / ml, preferably 0.0 1 mg / ml to 30 mg / ml, more preferably 0.0 lmg / m. 1 to: L Omg / m 1, most preferably 0.0 1 mg / m 1 to 3 mg / m 1 can be used as a food or drink.
  • L Omg / m 1 most preferably 0.0 1 mg / m 1 to 3 mg / m 1 can be used as a food or drink.
  • the polyphenol content is about 3 mg / m 1 or less.
  • sweet potato foliage extract in order to exert the medicinal effects / functions of sweet potato foliage extract, it is efficient to contain about 0.01 mg / m 1 or more. From these points, more preferably 0.05 mg / ml to 2.5 mg / m K, still more preferably 0.1 mg / m 1 to 2.5 mg / m K, particularly preferably 0.3 mg / ml to 2 mg / m Although it is 1, it can be appropriately increased or decreased depending on the type of food or drink to be processed. Also, the aqueous solution of sweet potato foliage extract is processed into confectionery such as jelly, the sweet potato foliage extract powder is mixed with processed foods such as potatoes and instant foods, and it is also used as a dietary supplement material You can also.
  • the amount of polyphenol derived from sweet potato foliage per preparation is adjusted to about 1 mg to 2000 rag, preferably about 5 mg to 500 mg, more preferably about 10 mg to 300 mg.
  • Extraction is performed by first leaving 700 ml of the extract at room temperature for 12 hours, followed by filtration, collecting the filtrate, and adding 500 ml of extract to the extraction residue. The extraction was performed for 12 hours. Thereafter, the same operation was performed, extraction was performed three times in total, and the collected extracts were combined.
  • the extract was concentrated to about 200 ml with a mouth-to-mouth evaporator and then fractionated with 50 ml of hexane to collect the aqueous layer portion.
  • DIAION HP20 sold by Mitsubishi Chemical.
  • a column was prepared by replacing 50 g of DIAION HP20 with water, and the collected aqueous layer was passed through the column. Thereafter, the column volume was washed with about 500 ml of water, and the polyphenol fraction was eluted with about 250 ral 90% ethanol and collected.
  • the polyphenol fraction collected was measured for the amount of polyphenol in terms of catechin using a polyphenol measuring device (PA-20) sold by Toyobo Engineering Co., Ltd. Effect of ethanol concentration on polyphenol extraction
  • Example 1 Separating the sweet potato leaves and extracting them with the method of Example 1 using 60% ethanol to obtain 200 ml of the concentrated solution. After leaving the concentrated solution for 3 days, the precipitate formed in 3 days was removed. Pass through the HP-20 column to remove the polyphenol fraction as in Example 1. Was collected. Then, the amount of polyphenol per dry weight of the fraction, ie purity, was examined. However, the hexane fraction in Example 1 was omitted as in (a).
  • Example 1 The sweet potato leaves were separated and extracted by the method of Example 1 using 60% ethanol to obtain 200 ml of concentrated solution. About 4 times the volume of ethanol was added to the concentrated solution and left for 3 days. After removing the precipitate formed in 3 days, the ethanol was again removed with a rotary evaporator, and the polyphenol fraction was collected in the same manner as in Example 1 through an HP-20 power ram. Then, the amount of polyphenol per dry weight of the fraction, ie purity, was examined. However, the hexane fraction in Example 1 was omitted as in (a).
  • FIG. 1 shows a liquid chromatography chart of the sweet potato foliage extract obtained by the method of Example 1 using 60% ethanol from sweet potato foliage. From the peak analysis, the extract contains quercetin 1-darcoside, quercetin-3-galactosid, chlorogenic acid and three dicaffeoylquinic acids (3,4-dicaffeoylquinic acid, 3,5-dicaffeoyl Quinic acid, 4,5-dicaffeoylquinic acid).
  • FIG. 2 shows a liquid mouth-matography chart of the sweet potato stem-and-leaf extract obtained by the method of Example 1 using 60% ethanol from sweet potato leaves. The extract using only the leaves was found to contain a lot of quercetin-1-3-glucosidase.
  • Example 4 Production of sweet potato stem and leaf extract using 80% ethanol
  • a sweet potato shoot extract was produced by the following method.
  • the resulting powder sample (Satsuma potato foliage extract) had a polyphenol content of approximately 34% in terms of strength, and the quercetin-3-darcoside content in the polyphenol was 5.6%. Further, it was confirmed by liquid chromatography that quercetin-1-darcoside, quercetin-1-galactoside, and chlorogenic acid dicaffeoylquinic acid were contained as in Example 3.
  • Example 5 Production of sweet potato stem and leaf extract using 60% ethanol
  • the precipitate (which seems to be polysaccharides, etc.) was removed by decantation, and the supernatant was concentrated under reduced pressure to a volume of about 1Z7 (990 ml) using a rotary evaporator.
  • the obtained aqueous solution was passed through a DIAION HP20 (Mitsubishi Chemical) column. After washing with 5 column volumes of water, the column was eluted with 90% ethanol of 2 column volumes, and the eluate was dried to dryness with a spray dryer to obtain a sweet potato stem and leaf extract.
  • the extract yield from dry foliage is 0.66%.
  • the content of liphenol was 41.3%, and the content of quercetin-3-glucoside in polyphenol was 3.0%. Further, it was confirmed by liquid chromatography that quercetin-1-3-dalcoside, quercetin-1-galactoside, chlorogenic acid and dicaffeoylquinic acid were contained as in Example 3.
  • Example 6 Comparison of extraction methods
  • the quercetin-3-darcoside extract was compared for 60% ethanol extraction, hot water extraction, and water extraction.
  • the dried sweet potato stems and leaves were extracted with 60% ethanol, concentrated with a rotary evaporator, applied to an HP20 column, the fraction eluted with 90% ethanol was dried, and the amount of quercetin-3-darcoside extracted was measured.
  • Extract the dried potato stems and leaves with hot water at 90 ° C add 2 volumes of 100% ethanol, remove the resulting precipitate, concentrate on a rotary evaporator, apply to an HP20 column, and elute with 90% ethanol The minutes were dried and the amount of quercetin-3-darcoside extracted was measured.
  • the dried sweet potato stems and leaves were extracted with hot water at 90 ° C, concentrated on a rotary evaporator, applied to an HP20 column, and the fraction eluted with 90% ethanol was dried to measure quercetin 1-darcoside extraction *.
  • Antioxidant ability was measured using sweet potato stem and leaf extract.
  • the superoxide scavenging activity of the sweet potato shoot extract obtained in Example 4 was measured by the electron spin reaction (ESR) method.
  • ESR electron spin reaction
  • the result was 3.9 ⁇ 10 4 units / g (units defined in J. Biol. Chera., 244, 6049 (1969)), confirming that the sweet potato polyphenol had antioxidant capacity. Although it has a high level of antioxidant capacity, it was about a quarter of that of Pycnogenol (1.6 X 10 5 units / g), which is said to have very high antioxidant capacity.
  • the D H HP radical scavenging ability of the sweet potato stem and leaf extract obtained in Example 4 and the stem-only extract or leaf-only extract obtained in Example 2 was measured by an electron spin reaction (ESR) method.
  • ESR electron spin reaction
  • Control group 5 animals, lmg / kg administration group: 5 animals, 30 mg / kg administration group: 5 animals
  • Body weight and food consumption measurement Sweet potato foliage extract was administered for 7 days, and the observed body weight and food consumption in the general state were measured daily from 10 to 11 am. Feed and water were ad libitum.
  • the sweet potato foliage extract obtained in Example 4 was dissolved in distilled water and orally administered using a stomach tube so that the intake of sweet potato foliage extract ⁇ ) was 1 mg / kg or 30 mg / kg per day. .
  • 10 mL / kg of physiological saline was administered.
  • sweet potato foliage extract and physiological saline were administered 12 hours before AAPH administration.
  • MPH was prepared at 50 mg / kg B.W using physiological saline and administered intraperitoneally to rats.
  • Urine samples were collected using metabolic cages after MPH administration until dissection. The collected urine was filtered under impurities and stored at -80 ° C until analysis.
  • test results were expressed as mean soil standard errors, and the Student's test was used for the significance test.
  • Serum total protein Control group 4. 88 ⁇ 0. 10 g / mL, lmg / kg group 5. 04 ⁇ 0.05 g / mL, 30 mg / kg group 5. 00 Sat 0.05 g / mL Indicated.
  • Serum albumin Control group 2. 70 ⁇ 0.05 g / mL, lmg / kg group
  • Serum total cholesterol Control group 40. 18 ⁇ 5. 55 mg / mU, lmg / kg group 55. 74 ⁇ 2. 93 mg / mL, 30 mg / kg group 48. 40 ⁇ 6. 41 mg / mL A slightly higher value was observed in the product administration group.
  • Serum HD L-cholesterol Control group 16. 20 ⁇ 1. 46 mg / mL, lmg / kg group 22. 20 ⁇ 0. 58 mg / mL, 30 mg / kg group 18. 20 ⁇ 2. 29 mg / mL Slightly higher in the foliage extract administration group, especially in the l mg / kg group.
  • Serum LD L-cholesterol Control group 2. 88 ⁇ 1. 29 mg / mL, lmg / kg group 5. 60 ⁇ 1. 29 mg / mL, 30 mg / kg group 4.00 ⁇ 1. 30 mg / mL Slightly higher in the foliage extract administration group, especially in the lmg / kg group.
  • Serum ALT Control group 70. 36 ⁇ 17 56 mg / mL, lmg / kg group 39.52 ⁇ 5.86 mg / raL, 30 mg / kg group 49.00 ⁇ 10.58 mg / mL, which was low in the sweet potato foliage extract administration group.
  • Liver T BARS Control group 0.486 ⁇ 0.035 nmol / mg protein, lmg / kg group 0.296 ⁇ 0.025 nmol / mg protein. Significant in 30 mg / kg group 0.286 ⁇ 0.020 nmol / mg protein and sweet potato foliage extract administration group (P ⁇ 0.01) showed a low value. The results are shown in Fig. 3 (b).
  • the results of measuring urinary 8—OH d G and liver T BAR S collected for 12 hours from the time of AAPH administration to the time of dissection showed that urinary 8 — OHdG and liver TBARS showed significantly lower values in the sweet potato foliage extract administration group compared to the Control group.
  • Macroscopic observation at the time of dissection showed that the condition was particularly good in the lmg / kg group, and the liver weight was also low.
  • the serum biochemical test values were similarly good in the sweet potato foliage extract administration group, and showed a tendency in the lmg / kg group rather than the 30 mg / kg group as in the macroscopic observation. In the 30 mg / kg group, clot peroxylipid and urine 8-0 H d G were lower than in the lmg / kg group.
  • the sweet potato foliage extract was found to be a serum lipid peroxidase and a liver T in the animal model in which in vivo lipid peroxidation was induced by intraperitoneal administration of the radical initiator AA PH.
  • BARS and urinary 8 An antioxidant that significantly suppresses the production of peroxides such as OHdG.
  • low-dose intake exhibits a milder antioxidant effect than high concentrations and reduces liver damage. It was confirmed that this material has an action to protect the liver.
  • As a polyphenol component it is expected to exhibit functionality with an intake of only about 20 mg per day.
  • tea catechins that have recently been in the spotlight A value of 500 mg has been published (Bioscience and Industry Vol. 61 No. 11 2003).
  • the functionality of tea catechin is to reduce body fat, and the functionality is different from that of the sweet potato stem and leaf extract of the present invention, but the effective concentration of polyphenol is an order of magnitude lower than tea catechin and is highly active. It exceeded our expectations.
  • the tyrosinase inhibitory activity was measured using the sweet potato stem and leaf extract obtained in Example 4. The measuring method is shown below.
  • Tyrosinase solution (2500 U / mg): Mushroom-derived tyrosinase (2500 U / mg) 6 mg. was made up to 10 mL with distilled water and used for measurement.
  • tyrosinase 50% inhibitory concentration (IC 5 ) of the test object was calculated.
  • the tyrosinase inhibitory activity of the sweet potato stem and leaf extract was measured in comparison with kojic acid and ascorbic acid, which are known to have a whitening effect. The results are shown in Table 5. Tyrosinase inhibitory effect of sweet potato stem and leaf extract
  • Table 5 shows that in vitro tyrosinase activity was measured for the tyrosinase activity of sweet potato stem and leaf extract, and the inhibition rate of tyrosinase activity was 66.40% at 0.05 mg, which was higher than kojic acid and ascorbic acid. showed that. From the results of the inhibition rate, IC 5 . Was estimated to be 0 ⁇ 037 mg. Example 1 1 Confirmation of sugar absorption inhibitory effect
  • Control group 5 animals, 30 mg / kg administration group: 5 animals
  • Body weight was measured daily between 10 am and 11 am only during the pre-breeding period.
  • glucose tolerance test was divided into 4 groups considering the weight of the previous day.
  • Sweet potato stem and leaf extract was prepared in distilled water at various concentrations and administered orally using a stomach tube.
  • glucose is orally administered at 2 g / kg after fasting for 18 hours from the day before the test.
  • Blood was collected from the orbital venous plexus before administration (0 minutes) and 30, 60, 90, and 120 minutes after administration, and measured using a blood glucose meter.
  • the sweet potato stem and leaf extract was applied 30 minutes before glucose load. Further, based on each measured value, a 120 minute ⁇ blood glucose area value (AUC: mg / dL ⁇ hr) was calculated from the following formula.
  • Tl Elapsed time (hr)
  • T2 1.0hr
  • T3 1.5hr
  • T4 2.0hr
  • test results are expressed as mean soil standard errors, and the Student's-1 test was used for the significant difference test.
  • Table 6 shows the results of the sugar tolerance test of the sweet potato stem and leaf extract. Blood sugar concentration by glucose tolerance test
  • test groups were sweet potato foliage extract 30 mg / kg group, 100 mg / kg group, guava leaf extract 100 mg / kg group, and control group, each with 5 rats.
  • Each test subject was prepared with physiological saline, and the control group was administered with physiological saline alone.
  • glucose was orally administered at 2 g / kg, blood was collected according to the same blood collection schedule as above, and blood glucose level was measured.
  • a graph of blood glucose concentration over time based on the measured values is shown in FIG.
  • Control group 5 animals, 30 mg / kg administration group: 5 animals
  • Body weight was measured daily between 10 am and 11 am only during the pre-breeding period. In addition, the fat tolerance test was divided into four groups considering the weight of the previous day.
  • the sweet potato foliage extract was adjusted to each concentration with distilled water and orally administered using a stomach tube.
  • the fat tolerance test was fasted for 18 hours from the day before the test, followed by oral administration of 2 g / kg of plant (soybean) oil, and before the administration (0 min) and after administration, 30, 60, 120, 180, 240, 300 min. Blood was collected from the venous plexus. The blood was centrifuged (4 ° C, 3000 rpm, 20 minutes) to collect plasma, and the triglyceride concentration was measured using a commercial kit. The sweet potato shoot extract was administered immediately before the vegetable oil administration. Moreover, based on each measured value, the ⁇ neutral fat area value (AUC: mg / dL ⁇ hr) for 300 minutes was calculated from the following formula.
  • AUC mg / dL ⁇ hr
  • AUC (0-T4) ClTl / 2 + (C1 + C2) (T2-T1) / 2 + (C2 + C3) (T3-T2) / 2 + (C3 + C4) (T4- T3) / 2 + (C4 + C5) ( ⁇ 5- T4) / 2 + (C5 + C6) ( ⁇ 6- ⁇ 5) / 2
  • Triglyceride increase from 0 to 30 minutes
  • Triglyceride increase from 0 to 240 minutes
  • Tl Elapsed time (hr)
  • T2 1.0hr
  • T3 2hr
  • T4 3hr
  • T5 4hr
  • T6 5hr
  • test results are expressed as mean soil standard error. Student 's-1 test was used, and it was considered significant at 0.05 compared with the control group.
  • Table 7 shows the results of the fat tolerance test of sweet potato stem and leaf extract. Neutral fat concentration by fat tolerance test
  • the sweet potato foliage extract administration group showed a lower value compared to the control group, and a significant difference was observed at 180 minutes. After 300 minutes, there was no difference between groups. Based on the above, the fat absorption inhibitory action of the sweet potato stem and leaf extract was confirmed, and the possibility of having an absorption delaying action is also considered.
  • a comparison test with a green tea extract (polyphenol content 30%) was conducted with respect to the fat absorption inhibitory effect.
  • the test groups were sweet potato foliage extract 30 mg / kg group, 100 mg / kg group, green tea extract 30 mg / kg group and control group, each of which was conducted in 5 rats.
  • Each test object was prepared with physiological saline, and the control group was administered with physiological saline alone.
  • 2 mg kg of plant (soybean) oil was orally administered after meals, and blood was collected according to the same blood collection schedule as above. The ride concentration was measured. Based on each measured value, one triglyceride area value (AUC: mg / dL-hr) for 300 minutes was calculated as described above and shown in FIG.
  • the sweet potato foliage extract administration group and the green tea extract administration group both significantly reduced the amount of neutral fat in the blood increased by intake of soybean oil. It was. Comparing the sweet potato foliage extract and the green tea extract to each 30 mg / kg group, it can be seen that the sweet potato foliage extract has a greater effect of reducing neutral fat in the blood. In the sweet potato foliage extract administration group, the amount of neutral fat was reduced in a dose-dependent manner. Based on the above, it was suggested that the sweet potato shoot extract exerts a stronger neutral fat absorption inhibitory effect than the green tea extract.
  • Example 4 To examine the oral toxicity of a single administration of the sweet potato foliage extract obtained in Example 4, at a dose of 0 mg / kg (control group) and 2000 mg / kg, each SD male and female 5 males and females. Was administered once and observed for 14 days. Administration solution were weighed predetermined amounts of Sammaimo foliage extracts were suspended in 2v / v% TV ee n80 aqueous is medium, and a dosing solution. The dosing solution was prepared once on the day of administration.
  • Measurement items include general condition observation (before administration, 30 minutes after administration, 1, 3, 5 hours, then once a day for 14 days), body weight measurement (immediately before administration, 2, 4, 8 and 15 days using an electronic pan balance), pathological examination (all patients were cut and exsanguinated on the 15th day under anesthesia by intraperitoneal administration of thiopental sodium, An autopsy was performed after euthanasia. None of the deaths occurred, and no toxic symptoms were observed during the observation period. Also, no abnormal findings were obtained at autopsy.
  • Example 1 4 Preparation of drinking water (hot water extract) containing sweet potato leaf extract
  • the sweet potato foliage extract By adding a roasting process, the sweet potato foliage extract can be tasted within the range that preserves the antioxidant properties of the sweet potato foliage extract.
  • the degree of roasting can be adjusted by adjusting the roasting temperature and time.
  • sweet potato foliage extract obtained in Example 4 (test sample) 500, 1000 and 2000 mg / kg was administered by gavage twice to male rats (7 weeks old, 5 animals / group) using 3 each dose (24 hour intervals As a result, there was no death in any group, and there was no change in the general condition. Therefore, in this study, the same concentration was used for test samples, and cyclophosphamide 10 mg / kg was added to positive subjects and physiological saline was added to negative subjects.
  • bone marrow cells were collected from the femurs of test animals to prepare tissue specimens.
  • the frequency of micronuclei was not statistically significant for the test sample, and mutagenicity was determined to be negative It was done.
  • Example 1 6 Deodorizing effect test of sweet potato stem and leaf extract
  • Example 1 7 Adipocyte differentiation and hypertrophy inhibition test using rat white adipocytes
  • adipocyte proliferation we conducted a study to inhibit the differentiation and hypertrophy of rat mesenteric white adipocytes in vitro.
  • adipocytes from Rat White Adipocyte Kit (Cell Garage Co., Ltd.) were used, and all operations were performed according to the usual method.
  • adipocytes were incubated in a growth medium under 5% CO 2 for 24 hours.
  • the cells were seeded on a 24-well plate so that the number of adipocytes was 2 ⁇ 10 5 cells / well, incubated for 24 hours, then changed to differentiation-inducing medium, and incubated for 48 hours under 5% C 0 2 .
  • 100 ⁇ g / m green tea extract (90% catechin) 100 gg / mL of the sweet potato leaf extract obtained in Example 4 was added to each adipocyte in the medium. It was added, nothing as a control which was not added, and the 5% C0 2 under incubation, observe the state of cells per 24h.
  • FIG. 9 shows the states of fat cells in each group at 24 hours, 72 hours, and 96 hours.
  • the sweet potato stem and leaf extract and the green tea extract both significantly suppressed the accumulation of fat cell fat droplets and fat cell hypertrophy over the entire time compared to the control. Comparing sweet potato stem and leaf extract with green tea extract, the accumulation of lipid droplets is slightly less after 24 hours, but after 48 hours (data not shown) and after 72 hours they are equivalent. After 96 hours, the both were equivalent, or slightly, the sweet potato shoot extract suppressed fat cell hypertrophy. From the above, it was found that the sweet potato stem and leaf extract is almost the same as or more effective than the green tea extract in that it has an effect of inhibiting adipocyte differentiation and hypertrophy. Therefore, it was suggested that the sweet potato foliage extract of the present invention can be used as a prophylactic / therapeutic agent for obesity caused by adipocyte differentiation and hypertrophy.
  • Example 1 Examination of antidepressant action and anti-fatigue action by forced swimming of mice
  • mice (BALB line * male * 7 weeks old) were acclimated for 7 days until the day before the start of this study.
  • Test animals were forced to swim for 5 minutes before the end of pre-breeding 2 Screening was performed so that the swimming state before administration was uniform, and taking into account the body weight of the last day of preliminary breeding, the animals were divided into groups of 6 animals, and this study was started.
  • the test groups were the sweet potato leaf extract 30 mg / kg group, the 300 mg / kg group, the St. John's single 300 mg / kg group obtained in Example 4 and the control group.
  • Each test object was prepared in physiological saline and administered by oral gavage (lOraL / kg) using a stomach tube.
  • physiological saline was similarly administered by oral gavage (10 mL / kg).
  • oral gavage 10 mL / kg.
  • One hour after administration of the test object it was allowed to swim in an acryl water tank (350 X 400 X 180 sleep) filled with 10 L of warm water (37 ° C), and the swimming state was observed.
  • Judgment was based on the following criteria.
  • EDB exit directed behavior
  • the average values of EDB and IMO time for each group are shown in FIG.
  • the sweet potato shoot extract and St. John's salt extract extract group a significant increase in EDB and a significant shortening of IM0 were observed compared to the control group.
  • the sweet potato foliage extract administration group showed a concentration-dependent effect, and the sweet potato foliage extract 300 mg / kg group had almost the same effect as the St. John's salt 300 mg / kg group.
  • St. John's wort is widely known as a herbal that relaxes and helps stress, and is actually used in Germany as an antidepressant for mild to moderate depressive symptoms.
  • the sweet potato foliage extract group was effective against forced exercise and mental stress in the same way as the St.
  • the sweet potato stem and leaf extract obtained in Example 4 was added to 3 g of vitamin E-free pork fat.
  • the sample solution was added to 200 ppm and lOOOOppm respectively.
  • the starting time of fat deterioration was determined using a dry oil flow rate of 20L / hr and a temperature of 120 ° C using an automatic oil stability tester (CDM tester).
  • Figure 11 shows the decay time of the sweet potato stem and leaf extract.
  • the sweet potato stem and leaf extract extended the decay time from 1.6 hours at Oppm to 200 times and the pork fat to 2 times (3.3 hours) and 3 times (5 hours) at lOOOppm.
  • the sweet potato stem and leaf extract is processed foods that use animal lipids such as pork fat and lard (fried potatoes, snacks, cups, etc.) and other various fats and oils to prevent deterioration It was suggested that it could be applied to.
  • Example 2 0 Preparation of a sweet potato stem and leaf extract
  • sweet potato stem and leaf extract of the present invention Using the sweet potato stem and leaf extract of the present invention, the following three types of preparations were prepared.
  • sweet potato stem and leaf extract is mixed with various excipients to a final concentration of 5%, a total amount of 2 kg of raw material is added and kneaded, and then spray-dried granulator Granules were prepared.
  • the manufactured granules were packaged in aluminum every 2 grams. Since this product is convenient to carry and has good water solubility, it can be made into a delicious beverage by dissolving it in hot water or water of about lOOml. This product contains 100 mg of sweet potato foliage extract per packet.
  • the sweet potato foliage extract can be mixed in each formulation as appropriate according to the purpose, and can be easily mixed with other functional ingredients, food and drink ingredients, and fragrances. Therefore, it was found that it is excellent in handling as a functional material and has a very wide range of applications. It can also be said that it can be used widely as a material for food and drink. Industrial applicability
  • the sweet potato stem and leaf extract of the present invention has such activities and functions as antioxidative activity, tyrosinase inhibitory activity, hepatoprotective activity, absorption inhibition activity of sugar and neutral fat, and so on. Useful as functional foods, functional materials, pharmaceuticals, etc.
  • a polyphenol-containing extract can be efficiently obtained without using any special materials or facilities.

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Abstract

Il est proposé de décrire une méthode avantageuse d’obtention d’un extrait contenant un polyphénol, qui possède des effets physiologiques divers tels qu’une action antioxydante, une action inhibitrice de la tyrosinase, un effet hépato protecteur et des effets inhibiteurs de sucre et d’absorption de graisse neutre, à partir d’une matière de base moins onéreuse. Plus spécialement, l’objet ci-dessus peut être établi en fournissant un extrait de pédoncule et de feuille de patate douce soluble dans l’eau, contenant un polyphénol, qui est obtenu à partir de pédoncule et de feuille de patate douce ; une méthode pour le produire ; et un aliment fonctionnel, une matière fonctionnelle, un antioxydant, un agent hépato protecteur, un inhibiteur de tyrosinase, un inhibiteur d’absorption de sucre, un inhibiteur d’absorption de graisse neutre et ainsi de suite, chacun contenant l’extrait ci-dessus de pédoncule et de feuille de patate douce.
PCT/JP2005/014799 2004-08-06 2005-08-05 Extrait de pédoncule de pomme de terre et utilisation WO2006014028A1 (fr)

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