WO2006118316A1 - Inhibiteur de l’accumulation de lipides dans le foie - Google Patents

Inhibiteur de l’accumulation de lipides dans le foie Download PDF

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Publication number
WO2006118316A1
WO2006118316A1 PCT/JP2006/309195 JP2006309195W WO2006118316A1 WO 2006118316 A1 WO2006118316 A1 WO 2006118316A1 JP 2006309195 W JP2006309195 W JP 2006309195W WO 2006118316 A1 WO2006118316 A1 WO 2006118316A1
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Prior art keywords
sweet potato
liver
leaves
lipid accumulation
foliage
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PCT/JP2006/309195
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English (en)
Japanese (ja)
Inventor
Kinya Takagaki
Original Assignee
Toyo Shinyaku Co., Ltd.
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Filing date
Publication date
Application filed by Toyo Shinyaku Co., Ltd. filed Critical Toyo Shinyaku Co., Ltd.
Publication of WO2006118316A1 publication Critical patent/WO2006118316A1/fr

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/39—Convolvulaceae (Morning-glory family), e.g. bindweed
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/06—Antihyperlipidemics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/12—Antihypertensives

Definitions

  • the present invention relates to a liver lipid accumulation inhibitor.
  • sweet potato stems and leaves contain health-effective ingredients such as vitamins and minerals, and have begun to be used as food ingredients for the prevention of lifestyle-related diseases (Japanese Patent Laid-Open No. 7-11). No. 8 78, and Japanese Unexamined Patent Publication No. 6-6 2 7 8 3).
  • sweet potato stems and leaves contain abundant active ingredients such as polyphenol, and further effective utilization of sweet potato stems and leaves is expected.
  • An object of the present invention is to provide a lipid accumulation inhibitor in the liver (hereinafter sometimes referred to as liver) containing a processed product of sweet potato foliage as an active ingredient. Is to expand.
  • the present inventor conducted intensive studies on the functionality of sweet potato foliage and found that the sweet potato foliage had an excellent inhibitory effect on lipid accumulation in the liver, thereby completing the present invention.
  • the liver lipid accumulation inhibitor of the present invention comprises a processed product of sweet potato stover.
  • the processed sweet potato stem and leaf product is a dried sweet potato stem and leaf extract, a sweet potato stem and leaf extract, or a sweet potato stem and leaf extract.
  • an excellent effect of suppressing accumulation of lipids in the liver can be obtained by ingesting a processed sweet potato stover.
  • the use of sweet potato foliage can be expanded by providing a processed product of sweet potato foliage as a liver lipid accumulation inhibitor.
  • the present invention provides a hepatic lipid accumulation inhibitor comprising a processed sweet potato stover as an active ingredient.
  • a processed sweet potato stover as an active ingredient.
  • the sweet potato stover or processed product thereof will be described first, and then the liver lipid accumulation inhibitor will be described.
  • the present invention is not limited to the following embodiment. That is, the present invention can be modified within the scope of the contents described in the claims.
  • the variety of sweet potato is not particularly limited.
  • the stem and leaves of “Suiou” which is a specific product type is preferably used.
  • “Suiou” has high polyphenol content, and even after harvesting the foliage once, the foliage Has the feature of playing.
  • Suio is rich in polyphenols even in the regenerated foliage. For these reasons, it is particularly preferred in the present invention to use a sweet potato stem and leaf.
  • sweet potato stem and leaf refers to “at least part of the sweet potato stem and sweet potato leaf”.
  • the sweet potato stalk may include any of the above-ground stalk and the underground stalk (underground stalk), but it is preferable to use the above-ground stalk.
  • the above-ground stems and leaves of sweet potato that is, the above-ground stems and leaves produced during sweet potato cultivation are particularly preferably used.
  • the size of the sweet potato stem and leaves used in the present invention is not particularly limited.
  • the length of the upper part of the sweet potato that is, the position where the stem of the sweet potato goes out of the ground (surface)
  • the length from the force to the tip of the sweet potato leaf is preferably 10 cm or more, more preferably Sweet potato stems and leaves of 30 cm or more, more preferably 60 cm or more are used.
  • a sweet potato stem and leaf having a length of the above-ground part of the sweet potato is preferably 300 cm or less, more preferably 20 00 cm or less, and even more preferably 150 cm or less.
  • the tip of the sweet potato stem and leaf becomes easy to touch the ground, making it susceptible to pests and the like, and as a result, a sufficient amount of sweet potato stem and leaf may not be harvested. There is.
  • sweet potato stover it is preferable to use a sweet potato stover in a state of maintaining a green color.
  • Such sweet potato stover and young leaves of sweet potato stover are easy to process because the plant itself is soft, and when processed into a dry powder or the like as it is, a dry powder with good touch can be obtained.
  • the sweet potato foliage used in the liver lipid accumulation inhibitor of the present invention comprises young shoots.
  • the “young stem leaf” of this sweet potato stem and leaf is a portion at the tip of the sweet potato stem and leaf that has a yellowish green color compared to other stems and leaves.
  • the site is preferably 60 cm or less from the most distal stem or leaf.
  • This sweet potato shoot is particularly preferably used because it contains a large amount of polyphenol.
  • the sweet potato stalks and leaves used in the present invention are usually subjected to processing after washing mud or the like with water.
  • the processed sweet potato foliage used in the present invention is obtained by processing the above sweet potato foliage or a washed product thereof, for example, at least one selected from the group consisting of calo heat treatment, pressing treatment, drying, pulverization treatment, and extraction Obtained by.
  • the sweet potato stover or the major axis l mn as required! ⁇ 30 cm of dried product cut and dried (dried powder), sweet potato stem juice or crushed and solid-liquid separated supernatant (these are called sweet potato stem extract) and the above juice or Examples include an extract powder obtained by drying the supernatant, and an extract obtained by extracting sweet potato stems and leaves with water or an organic solvent (particularly, a polar solvent).
  • the above-described processing hereinafter, the above-described processing (heating treatment, pressing treatment, smashing treatment, extraction, drying, and powdering treatment) will be described.
  • the heat treatment is performed in order to inactivate the enzymes in the sweet potato stalks and stabilize the quality, or to prevent the sweet potato stalks from fading.
  • Examples of the heat treatment include a branching treatment (blanching), a dry heat treatment, a microwave treatment, an infrared treatment or a far infrared treatment, and a steam treatment.
  • a branching treatment blanching
  • a dry heat treatment a microwave treatment
  • an infrared treatment or a far infrared treatment a steam treatment.
  • a steam treatment a method usually performed by a person skilled in the art for preventing the discoloration of the color of chlorophyll, which is a pigment of a green plant, in particular, blanching is suitably employed.
  • the heat treatment can be appropriately performed on any sweet potato stover or its crushed material or processed product thereof (for example, squeezed juice, dried powder, dried extract powder, etc.).
  • the sweet potato leaves are heat-treated as they are, the major axis is l mn
  • the conditions for the heat treatment vary greatly depending on the type of sweet potato. Furthermore, there is a problem that flavor and nutrients are impaired, and the physiological activity of useful ingredients is inactivated. It is easy.
  • the heat treatment is performed at a pH of 5.4 or more, preferably pH 5.6 or more and less than 8.4, more preferably ⁇ 5.6 to 8.0, most preferably ⁇ 5.6 to 7.6. .
  • a processed sweet potato foliage with a high content of an active ingredient for example, polyphenol
  • a processed sweet potato foliage with a high antioxidant activity can be obtained efficiently.
  • hot water having the above pH from the viewpoint of obtaining a sweet potato foliage product having a high content of the above active ingredients.
  • microwave treatment, infrared treatment or far-infrared treatment, or steam treatment perform treatment such as spraying a solution adjusted to ⁇ in the above range on the sweet potato foliage before heating. It is preferable to process.
  • the pH is adjusted by a method commonly used by those skilled in the art. For example, when adjusting to basic, sodium hydroxide hydrate, baking soda, calcium carbonate (eg eggshell calcium, scallop shell calcium, coral calcium, etc.), oxidation obtained by baking the above-mentioned carbonated lucium as a pH adjuster Calcium is used. Alkaline ion water adjusted to a predetermined pH may be used. When adjusting to acidity, organic acids such as acetic acid, citrate, ascorbic acid, tartaric acid, malic acid and fumaric acid are used. The amount of these pH adjusters is appropriately set according to the type of t) H adjuster.
  • the pH adjusted to the hot water or solution from 0.01 to 5 wt%, preferably 0.2 to 3 mass 0/0 saline It is preferable to add.
  • sodium chloride By adding sodium chloride in this way, it is possible to obtain a processed sweet potato foliage with a more vivid green color and a good flavor.
  • the heating temperature is higher than 80 ° C, preferably 90 ° C or higher.
  • the heating time is less than 5 minutes, preferably 3 minutes or less, most preferably 10 seconds to 3 minutes. ⁇ ⁇ It is preferable to cool immediately after the heat treatment in order to maintain the green color and flavor of the sweet potato leaves. Cooling is performed by means usually used by those skilled in the art, such as immersing in cooling water or applying rapid cooling to cool air. The lower the cooling temperature, the brighter the green color of the processed sweet potato stems and leaves, the more beautiful it looks. For example, when immersed in cooling water, use water at 30 ° C or lower, preferably water at 20 ° C or lower.
  • the cooling time may be set according to the amount of sweet potato stems and leaves processed. It is preferable to carry out the cooling process until the sweet potato stems themselves reach a temperature equivalent to the cooling temperature.
  • the squeezing treatment or rupture treatment is performed for the purpose of obtaining the juice or supernatant of the sweet potato stems and leaves.
  • the squeezed juice is obtained by using the pressing machine or the like for the sweet potato foliage as it is or for the heat-treated foliage.
  • the crushing treatment for example, the paste is obtained by using the above-mentioned foliage and the like using a mascot mouth lid. This paste (debris) is then made into a supernatant by solid-liquid separation (eg, filtration). From the viewpoint of storage, it is preferable to sterilize the juice or supernatant of the sweet potato stover obtained in this way at 80 ° C. to 130 ° C.
  • Extraction is performed, for example, by adding a solvent to a processed sweet potato foliage such as a freshly cut sweet potato foliage or a dried sweet potato foliage (powder) and holding it at a predetermined temperature.
  • a solvent include water (hot water), organic solvents (particularly polar organic solvents such as ethanol and methanol), hydrous organic solvents (eg, hydrous alcohols such as hydrous ethanol), and the like.
  • the water-containing organic solvent is preferably used from the standpoint of efficiently extracting an active ingredient (for example, polyphenol).
  • an active ingredient for example, polyphenol
  • Preferred is a hydrous polar organic solvent, more preferred is a hydrous alcohol, and more preferred is hydrous ethanol.
  • the ethanol preferably has an ethanol content of 10 to 90% by volume.
  • the amount of the extraction solvent is not particularly limited. Usually, 0.5 parts by mass to 50 parts by mass of the extraction solvent is added to 1 part by mass of sweet potato stems and leaves.
  • extraction temperature suitably according to the kind of extraction solvent.
  • it is 4 ° C to 100 ° C, preferably 10 ° C to 100 ° C.
  • the extraction time is, for example, 1
  • the extraction time is set to 30 minutes or less
  • the extract obtained in this manner was further synthesized with a synthetic adsorbent (Diaion (registered trademark) HP 20, Sephabies SP 8 25, Amperlite (registered trademark) XAD 4, MC Ige 1 CHP 2
  • the active ingredient eg, polyphenol
  • the above-mentioned drying and flour treatment are performed for the purpose of obtaining a dried product of sweet potato stems and leaves, a dried powder, and a dried extract powder.
  • Drying is performed for the purpose of long-term storage, the purpose of preventing quality deterioration and increasing the value of products.
  • the drying method is not particularly limited.
  • a hot air dryer, a high-pressure steam dryer, an electromagnetic wave dryer, a freeze dryer, an open flame heater, a rotary dryer, or the like is used. From the viewpoint of manufacturing cost and drying efficiency, it is preferable to use a hot air dryer, a direct-fired heater, or a rotary draft dryer.
  • drying liquids such as sugar cane squeezed juice, vacuum concentration, spray drying, etc. can be performed. From the viewpoint of production cost and drying efficiency, vacuum concentration is preferred.
  • the drying temperature should be about 60 to 150 ° C under normal pressure.
  • drying can be performed while reducing loss of nutrient components, preferably at 60 ° C. or lower and under pressure at which sweet potato foliage, paste, or juice is not frozen. Drying is usually performed with the moisture content in the dried product or extract powder being 5% by mass or less.
  • the sweet potato stems and leaves may be dried as they are, but it is preferable to perform two-stage drying. By performing this two-stage drying process, the drying time is shortened and the green color and flavor of the sweet potato leaves are maintained.
  • the sweet potato stover or processed product of sweet potato stover is firstly dried at a temperature of 60 to 80 ° C. until the water content becomes 25 mass ° / 0 or less. Then, until the water content of the primary dried sweet potato leaves becomes 5% by mass or less, the temperature is higher than that of the primary drying, and is not higher than 100 ° C, preferably not higher than 90 ° C, more preferably not higher than 80 ° C.
  • the temperature difference between the primary drying and the secondary drying is preferably about 5 to 15 ° C, and preferably about 10 ° C. More preferred.
  • the spray drying is performed using a spray dryer such as a spray dryer when, for example, squeezed sweet potato leaves are used as the extract powder.
  • a spray dryer such as a spray dryer when, for example, squeezed sweet potato leaves are used as the extract powder.
  • excipients such as dextrin, cyclodextrin, starch, maltose, etc. are used as needed to increase recovery.
  • dextrin is used, and the ratio of squeezed to dextrin facilitates powdering. Therefore, the mass ratio is preferably 1:10 to 5: 1.
  • a dried sweet potato leaf (dried powder) is obtained from a sweet potato stem or a sweet potato leaf heat-treated product, and a dried sweet potato leaf extract is obtained from the sweet potato juice.
  • a dried sweet potato stover obtained by drying a heat-treated sweet potato leaf is preferably used as the processed sweet potato.
  • the powder cake treatment is carried out for the purpose of pulverizing the dried sweet potato stover, or for the purpose of further pulverizing the dried powder.
  • By grinding it is possible to obtain a uniform fine powder with a smaller particle size. Since sweet potato stalks and leaves include stem parts, leaf parts, and petiole parts having different hardness, it is preferable to go through a coarse mash process and a fine mash process from the viewpoint of increasing the efficiency of the mash.
  • the coarse powder koji is cut with any machine or tool normally used by those skilled in the art, such as a cutter, a slicer, a dicer, etc.
  • the size of the dried material of cut sweet potato stems and leaves has a major axis of 2 O mm or less, preferably 0.1 to 10 mm. Note that this step can be omitted if the paste has been crushed in advance.
  • the dried powder of sweet potato stover in a coarse powder frame is pulverized. It may be sterilized in advance before the pulverization step. By applying sterilization, the coarsely-powdered sweet potato foliage can be heated uniformly, and the sterilization of the sweet potato foliage can be improved and efficient sterilization can be performed. Sterilization is performed at 11 o ° C or higher using a high-pressure sterilizer, heat sterilizer, or pressurized steam sterilizer. For example, if the sterilization by pressurized steam sterilization, sweet potato foliage workpiece was coarsely pulverized, for example, 0. 5 to 1 0 kg Bruno cm 2 of pressure, 1 1 0 ⁇ 2 0 0 ° C of saturated steam Then, heat treatment is performed for 2 to 10 seconds. If necessary, the moisture contained during heating with saturated steam is further dried.
  • the fine grinding is preferably ground so that 90% by weight passes through the 200 mesh section.
  • crusher, mill, blender, millstone This is done using any machine or tool commonly used by any person skilled in the art.
  • a fine powder monument improves palatability (texture).
  • the palatability is further improved, and when the obtained fine powder is added to food, it is easily mixed.
  • the hepatic lipid accumulation inhibitor of the present invention comprises the above processed product of sweet potato stover.
  • This processed sweet potato foliage has an excellent effect of suppressing the accumulation of lipids in the liver, and can be used for the prevention or treatment of non-alcoholic fats.
  • the intake of the liver lipid accumulation inhibitor of the present invention is not particularly limited. Ingest so that the amount of sweet potato foliage per day for adults is 10 to 300 mg, preferably 10 to 100 mg, as the total amount of polyphenol It is preferable. When dry powder of sweet potato foliage is used as a processed product of sweet potato foliage, a powder amount of 0.1 to 30 g, preferably 0.1 to 10 g, may be ingested.
  • the liver lipid accumulation inhibitor of the present invention is mixed with an excipient, a bulking agent, a binder, a thickener, an emulsifier, a coloring agent, a fragrance, a food ingredient, a seasoning, a pharmaceutical ingredient, etc., as necessary. It can also be taken in the form of food, medicine, etc.
  • Examples of food ingredients that can be added to such foods include royal jelly, propolis, vitamins (A, C, D, E, K, folic acid, pantothenic acid, biotin, derivatives thereof, etc.), minerals (iron , Magnesium, calcium, dumbbell, selenium, etc.), ⁇ -lipoic acid, lecithin, polyphenol (flavonoids, derivatives of these), carotenoids (lycopene, fastaxanthin, zeaxanthin, rutin, etc.), xanthine derivatives (caffeine) Etc.), Fatty acids, Proteins (Collagen, Elastin, etc.), Mucopolysaccharides (Hyaluronic acid, etc.), Amino sugars (Darcosamine, Acetyldarcosamine, Galatato Samine, acetyl galatatosamine, neuraminic acid, acetyl neuraminic acid, hexosamine, salts thereof, oligos
  • liver lipid accumulation inhibitor of the present invention When the liver lipid accumulation inhibitor of the present invention is used in the form of food or medicine, granules, tablets, capsules (hard capsule, soft capsule, etc.), pills, powder, liquid, tea It is processed into a bag-like shape or a bowl-like shape.
  • the liver lipid accumulation inhibitor processed in this way may be eaten as it is, or may be taken by dissolving in water, hot water, milk or the like. If powder or the like is provided in the form of a tea bag, the extract obtained by soaking in hot water can be used as a beverage.
  • green leaf powders such as wheat leaves (eg, barley leaves), kale, tomorrow leaves, and mulberry leaves, which are raw materials for green juice
  • the liver lipid accumulation inhibitor of the present invention to form a green juice.
  • the taste of traditional green juice is improved, making it easier to take.
  • Suio seeds were planted and cultivated until the length of the above-ground part (the length from the position where the stem of the sweet potato came out from the ground to the tip of the sweet potato stem) reached about 1550 cm. A 60 cm portion was cut from the tip of the sweet potato leaf and washed twice with water to obtain a sweet potato stem leaf containing 1 kg of sweet potato leaf.
  • the crushed sweet potato foliage was immersed in 2 L of hot water (90 ° C) adjusted to pH 8.0 for 1 minute for blanching treatment. went. Next, after cooling with 25 ° C. water, it was centrifuged for 30 seconds to dehydrate it to some extent. Thereafter, hot air drying (primary drying) was performed in a dryer at 70 ° C. for 2 hours until the water content reached about 20% by mass. Next, hot air drying (secondary drying) was performed at 80 ° C. for 4 hours so that the final moisture content was 3% by mass. The dried product was sterilized with steam using a saturated water pressure of 150 ° C. for 3 seconds. Moisture adhering to the sweet potato stems and leaves was again removed by sterilization, and then finely ground using a hammer mill so that 90% by mass passed through the 200 mesh fraction, and 80 g of sweet potato stems and leaves Of dry powder was obtained.
  • Example 3 Production of sweet potato stover leaf extract powder
  • Example 1 the dry powder of sweet potato stover obtained in Example 1 (Toyo Shinyaku Co., Ltd.) was 2% by mass, cholesterol was 1% by mass, and cholic acid was 0.25% by mass.
  • a test feed was prepared by adding to the feed (MF feed, Oriental Yeast Co., Ltd.).
  • a comparative feed (high-cholesterol-loaded feed) was prepared in the same manner as the above test feed except that the dry powder of sweet potato stover was not added.
  • a standard diet (MF feed, Oriental Yeast Co., Ltd.) was given to eight 5-week-old male SD rats (Nippon Chiarus Ribaichi Co., Ltd.) and acclimated for one week. Next, the group was divided into three groups of six per group so that the average body weight per group was uniform. One group of rats received the test diet (test group 1), the other group received the comparative diet (comparison group 1), and the other group of rats received the standard diet (control group 1). Ad libitum.
  • the value is the average soil standard deviation
  • test group 1 that received a high cholesterol-loaded feed mixed with dry powder of sweet potato stover (test feed)
  • the amount of triglycerides and total cholesterol in the monthly collection were lower than those in the comparison group that took the (comparative feed).
  • rats in test group 1 had lower levels of triglycerides in the liver than those in control group 1 that received only the standard diet.
  • the processed sweet potato foliage has an action of suppressing the accumulation of neutral fat and cholesterol in the liver and is effective in the prevention or treatment of fatty liver.
  • Example 1 Using the dried powder of sweet potato stover obtained in Example 1, the effect of inhibiting lipid accumulation in the liver was verified by the following method.
  • He pG2 cells human liver tumor cells (obtained from RIKEN) were seeded on 96-well plates to give 2 X 10 4 ce 1 1 s Z-well, and 0.1 mL of standard medium (10 In a DMEM medium containing vol% urchin fetal serum), the cells were cultured at 37 ° C. in a 5% CO 2 atmosphere for 24 hours.
  • the culture solution was removed from the wells, each well was washed three times with phosphate buffered saline (PBS), and 100 ⁇ L of PBS was added to each well.
  • PBS phosphate buffered saline
  • the cell membrane was then disrupted by freezing and melting the well.
  • the amount of triglyceride contained in the obtained cell lysate was measured using a triglyceride ⁇ Test ⁇ Co (Wako Pure Chemical Industries, Ltd.).
  • the amount of the protein contained in the obtained cell lysate was measured using Protein Rapid Sakai (manufactured by Wako Pure Chemical Industries, Ltd.). From these measurement results, the amount of triglyceride per mg of protein was determined.
  • Comparative group 2 was cultured in the same procedure as test group 2 except that dry powder of mugwort (Matsusho Co., Ltd.) was used instead of the dry powder of sweet potato stover. Then, in the same procedure as in Test Group 2, the amount of tridallylide per lmg protein was determined.
  • Comparative group 3 was cultured in the same procedure as test group 2, except that the dry powder of sweet potato stover was not used.
  • the amount of triglyceride per lmg protein was determined in the same procedure as in test group 2.
  • Control group 2 was cultured in the same procedure as in test group 2, except that the oleic acid and the dry powder of sweet potato stover were not used. Then, the amount of triglyceride per mg of protein was determined in the same procedure as in test group 2.
  • Values are percentages when control group 2 is 100
  • the amount of triglyceride in the test group 2 Hep G 2 cells cultured in a medium containing dry powder of sweet potato stover was lower than that in the comparative group 3 cells cultured in a medium containing only oleic acid.
  • Hep G 2 cells in Test Group 2 were compared to Comparative Group 2 ⁇ H ep G 2 cells cultured in a medium containing dried mugwort powder (including chlorogenic acids polyphenols as well as sweet potato leaves).
  • the amount of triglyceride per mg protein was low. That is, it can be seen that the processed sweet potato foliage has the action of suppressing the accumulation of tridaliselide (neutral fat) in the liver.
  • the hepatic lipid accumulation inhibitor containing the processed sweet potato stem and leaf product of the present invention as an active ingredient suppresses the accumulation of fat in the liver, and thus can exert a preventive effect on, for example, fatty liver.
  • prevention of fatty liver can also be expected to have preventive effects such as diabetes, hyperlipidemia, hypertension, and arteriosclerosis, which have been pointed out to increase the risk of fatty liver.
  • the hepatic lipid accumulation inhibitor of the present invention is useful as a food, a pharmaceutical or the like. . ' ⁇

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Abstract

La présente invention concerne un inhibiteur d’accumulation de lipides dans le foie, comprenant un produit traité à base de feuilles et tiges de patate douce en tant que principe actif. L’inhibiteur d’accumulation de lipides dans le foie selon l’invention présentant d’excellents effets d'inhibition de l’accumulation de graisse neutre et de cholestérol total dans le foie, il peut prévenir ou améliorer la stéatose hépatique et réduire les risques de cirrhose du foie, de diabètes, d’hyperlipémie, d’hypertension, d’artériosclérose et similaire. Ainsi, l’inhibiteur d’accumulation de lipides dans le foie, conformément à cette description, peut étendre la plage d’application des feuilles et tiges de patate douce.
PCT/JP2006/309195 2005-04-27 2006-04-26 Inhibiteur de l’accumulation de lipides dans le foie WO2006118316A1 (fr)

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JP2005-130513 2005-04-27
JP2005130513A JP2008208030A (ja) 2005-04-27 2005-04-27 肝中脂質蓄積抑制剤

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WO2006118316A1 true WO2006118316A1 (fr) 2006-11-09

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JP4738464B2 (ja) * 2008-10-10 2011-08-03 武義 浦田 アディポネクチン分泌促進用飲食品
JP5794678B2 (ja) * 2010-06-16 2015-10-14 株式会社東洋新薬 グルカゴン様ペプチド−１分泌促進剤
JP5412384B2 (ja) * 2010-08-12 2014-02-12 億 太院 茶茎葉及び／又はサツマイモ茎葉を用いた発酵食品及びその製造方法
JP2013202005A (ja) * 2012-03-29 2013-10-07 National Agriculture & Food Research Organization クルクミン類含有油脂およびその製造方法

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JP2004350619A (ja) * 2003-05-30 2004-12-16 Denen Shuzo Kk カフェ酸の製造方法
WO2006014028A1 (fr) * 2004-08-06 2006-02-09 Mochida Pharmaceutical Co., Ltd. Extrait de pédoncule de pomme de terre et utilisation

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JP2004350619A (ja) * 2003-05-30 2004-12-16 Denen Shuzo Kk カフェ酸の製造方法
CN1476842A (zh) * 2003-07-15 2004-02-25 中国人民解放军第二军医大学 灯盏花素和二咖啡酰奎宁酸药物组合物及其应用
WO2006014028A1 (fr) * 2004-08-06 2006-02-09 Mochida Pharmaceutical Co., Ltd. Extrait de pédoncule de pomme de terre et utilisation

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