Classifications

• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D213/00—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members
  • C07D213/02—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members
  • C07D213/04—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom
  • C07D213/60—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
  • C07D213/62—Oxygen or sulfur atoms
  • C07D213/63—One oxygen atom
  • C07D213/64—One oxygen atom attached in position 2 or 6
  • C07D213/643—2-Phenoxypyridines; Derivatives thereof
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
  • A61P1/04—Drugs for disorders of the alimentary tract or the digestive system for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
  • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P11/00—Drugs for disorders of the respiratory system
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P11/00—Drugs for disorders of the respiratory system
  • A61P11/02—Nasal agents, e.g. decongestants
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P11/00—Drugs for disorders of the respiratory system
  • A61P11/06—Antiasthmatics
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P13/00—Drugs for disorders of the urinary system
  • A61P13/12—Drugs for disorders of the urinary system of the kidneys
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P17/00—Drugs for dermatological disorders
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P17/00—Drugs for dermatological disorders
  • A61P17/06—Antipsoriatics
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P19/00—Drugs for skeletal disorders
  • A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
  • A61P19/10—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease for osteoporosis
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P25/00—Drugs for disorders of the nervous system
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P27/00—Drugs for disorders of the senses
  • A61P27/02—Ophthalmic agents
  • A61P27/06—Antiglaucoma agents or miotics
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P3/00—Drugs for disorders of the metabolism
  • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
  • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P35/00—Antineoplastic agents
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P37/00—Drugs for immunological or allergic disorders
  • A61P37/08—Antiallergic agents
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P9/00—Drugs for disorders of the cardiovascular system
  • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
  • C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
  • C07D209/04—Indoles; Hydrogenated indoles
  • C07D209/10—Indoles; Hydrogenated indoles with substituted hydrocarbon radicals attached to carbon atoms of the hetero ring
  • C07D209/12—Radicals substituted by oxygen atoms
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
  • C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
  • C07D209/04—Indoles; Hydrogenated indoles
  • C07D209/10—Indoles; Hydrogenated indoles with substituted hydrocarbon radicals attached to carbon atoms of the hetero ring
  • C07D209/14—Radicals substituted by nitrogen atoms, not forming part of a nitro radical
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
  • C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
  • C07D209/04—Indoles; Hydrogenated indoles
  • C07D209/30—Indoles; Hydrogenated indoles with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, directly attached to carbon atoms of the hetero ring
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D213/00—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members
  • C07D213/02—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members
  • C07D213/04—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom
  • C07D213/60—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
  • C07D213/62—Oxygen or sulfur atoms
  • C07D213/70—Sulfur atoms
  • C07D213/71—Sulfur atoms to which a second hetero atom is attached
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D307/00—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom
  • C07D307/02—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings
  • C07D307/34—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members
  • C07D307/56—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
  • C07D307/64—Sulfur atoms
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D307/00—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom
  • C07D307/77—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom ortho- or peri-condensed with carbocyclic rings or ring systems
  • C07D307/78—Benzo [b] furans; Hydrogenated benzo [b] furans
  • C07D307/79—Benzo [b] furans; Hydrogenated benzo [b] furans with only hydrogen atoms, hydrocarbon or substituted hydrocarbon radicals, directly attached to carbon atoms of the hetero ring
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D333/00—Heterocyclic compounds containing five-membered rings having one sulfur atom as the only ring hetero atom
  • C07D333/02—Heterocyclic compounds containing five-membered rings having one sulfur atom as the only ring hetero atom not condensed with other rings
  • C07D333/04—Heterocyclic compounds containing five-membered rings having one sulfur atom as the only ring hetero atom not condensed with other rings not substituted on the ring sulphur atom
  • C07D333/26—Heterocyclic compounds containing five-membered rings having one sulfur atom as the only ring hetero atom not condensed with other rings not substituted on the ring sulphur atom with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
  • C07D333/30—Hetero atoms other than halogen
  • C07D333/34—Sulfur atoms
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
  • C07D401/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings
  • C07D401/12—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings linked by a chain containing hetero atoms as chain links
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D407/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having oxygen atoms as the only ring hetero atoms, not provided for by group C07D405/00
  • C07D407/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having oxygen atoms as the only ring hetero atoms, not provided for by group C07D405/00 containing two hetero rings
  • C07D407/12—Heterocyclic compounds containing two or more hetero rings, at least one ring having oxygen atoms as the only ring hetero atoms, not provided for by group C07D405/00 containing two hetero rings linked by a chain containing hetero atoms as chain links

Definitions

• the present invention relates to cannabinoid receptor agonists which bind to cannabinoid (CB 2 ) receptors.
• Compounds according to the present invention generally exhibit anti-inflammatory and immunomodulatory activity and are useful in treating conditions characterized by inflammation and immunomodulatory irregularities. Examples of conditions which can be treated include, but are not limited to, rheumatoid arthritis, asthma, allergy, psoriasis, Crohn's disease, systemic lupus erythematosus, multiple sclerosis, diabetes, cancer, glaucoma, osteoporosis, renal ischemia, cerebral stroke, cerebral ischemia, and nephritis.
• the invention also relates to pharmaceutical compositions containing said compounds.
• Cannabinoid receptors belong to the superfamily of G-protein coupled receptors. They are classified into the predominantly neuronal CB-i receptors and the predominantly peripheral CB 2 receptors. These receptors exert their biological actions by modulating adenylate cyclase and Ca +2 and K + currents. While the effects of CBi receptors are principally associated with the central nervous system, CB 2 receptors are believed to have peripheral effects related to bronchial constriction, immunomodulation and inflammation.
• the present invention relates to compounds of formula I:
• R 1 is selected from the group consisting of hydrogen, substituted or unsubstituted alkyl, substituted or unsubstituted alkenyl, haloalkyl, -N(R 7 ) 2 , substituted or unsubstituted cycloalkyl, substituted or unsubstituted cycloheteroalkyl, substituted or unsubstituted aryl, and substituted or unsubstituted heteroaryl, wherein the term "substituted” means being substituted with (X) p ;
• R 2 is selected from the group consisting of hydrogen, substituted or unsubstituted alkyl, substituted or unsubstituted alkenyl, haloalkyl, -N(R 7 ) , substituted or unsubstituted cycloalkyl, substituted or unsubstituted cycloheteroalkyl, substituted or unsubstituted aryl, and substituted or unsubstituted heteroaryl, wherein the term "substituted” means being substituted with (X) p ;
• R 3 is selected from the group consisting of alkyl, heteroalkyl, aryl, heteroaryl, Br, CI, F, CF 3 , OCF 2 H, OCF 3 , and alkoxy, wherein R 3 can be the same or different and is independently selected when n > 1 ;
• R 4 is selected from the group consisting of H, substituted or unsubstituted alkyl, substituted or unsubstituted alkenyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted cycloheteroalkyl, substituted or unsubstituted aryl, and substituted or unsubstituted heteroaryl, wherein the term "substituted” means being substituted with (X) p ;
• R 5 and R 6 which can be the same or different, are independently selected from the group consisting of H, substituted or unsubstituted alkyl, substituted or unsubstituted alkenyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted cycloheteroalkyl, substituted or unsubstituted aryl, and substituted or unsubstituted heteroaryl, wherein the term "substituted" means being substituted with
• R 7 is selected from the group consisting of hydrogen, substituted or unsubstituted alkyl, substituted or unsubstituted alkenyl, haloalkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted cycloheteroalkyl, substituted or unsubstituted aryl, and substituted or unsubstituted heteroaryl, or two R 7 groups can form a ring of 4-7 carbon atoms, wherein the term "substituted" means being substituted with (X) p ;
• L 2 is selected from the group consisting of a covalent bond, -CH 2 -, -CH(CH 3 )-, ⁇
• M is a heteroaryl moiety; n is 0-4; p is 0-5;
• X can be the same or different and is selected from the group consisting of Br, CI, F, CF 3 , OH, OCF 2 H, OCF 3 , alkoxy, alkyl, cycloalkyl, -O-cycloalkyl, heteroalkyl, - C(O)N(R 7 ) 2 , -S(0 2 )R 2 , and -OS(0 2 )R 2 , wherein X is independently selected when p >
• Y is selected from the group consisting of a covalent bond, -CH 2 -,
• Z is selected from the group consisting of a covalent bond, -CH 2 -, -S(O )-, and -C(O)-, with the following provisos: when L 2 is a covalent bond, M is directly linked to R 4 ; when Y is a covalent bond, R 1 is directly linked to the nitrogen atom shown in formula I; and when Z is a covalent bond, R 2 is directly linked to the nitrogen atom shown in formula I.
• CB 2 receptors in a patient in need of such stimulation comprising administering to a patient having CB 2 receptors a CB 2 receptor stimulating amount of one or more compounds of formula I.
• Another aspect of the invention relates to a method of treating cancer, inflammatory diseases, immunomodulatory diseases, or respiratory diseases comprising administering to a patient in need of such treatment one or more compounds of formula I.
• Another aspect of the invention relates to a method of treating multiple sclerosis comprising co-administering or combining with the compound of formula I one or more additional agents which may be the same or different and are independently selected from the group consisting of Interferon B1a, Interferon B1 b and glatiramer acetate.
• kits for treating cancer, inflammatory diseases, immunomodulatory diseases, and respiratory diseases comprising in one or more containers an active compound for stimulating cannabinoid CB 2 receptors in a patient in need of such stimulation which comprises in one or more containers, one or more compounds according to claim 1 in one or more pharmaceutically acceptable carriers.
• the present invention relates to cannabinoid receptor antagonist compounds of formula I: or a pharmaceutically acceptable salt or solvate thereof; wherein R 1 , R 2 , R 3 , R 4 , R 5 , R 6 , L 1 , L 2 , M, n, p, X, Y, and Z are as defined above.
• L 1 preferably represents -(SO 2 )-, -CH 2 -, or -CH(OH)-.
• L 2 preferably represents -(SO )-.
• n is preferably 0.
• R 1 preferably represents CH 3 or CF 3 .
• R 2 preferably represents H.
• R 4 preferably represents furanyl, pyridyl, thienyl, quinolyl or fluorophenyl.
• R 5 and R 6 which can be the same or different, preferably represent H or CH 3 .
• X preferably represents CI or F.
• M is preferably selected from the group consisting of indolyl, benzofuranyl, dihydrobenzofuranyl, furanyl, thienyl, pyridinyl, aryl and pyridinyl-N-oxide. In another embodiment, M is selected from the group consisting of pyridinyl, pyridinyl-N-oxide, furanyl and thienyl.
• Y preferably represents -S(O 2 )- or -C(O)-.
• the compound of the present invention is represented by the formula:
• the compound of the present invention is represented by the formula:
• the compound of the present invention is represented by the formula:
• the compound of the present invention is represented by the formula:
• the compound of the present invention is represented by the formula:
• the compound of the present invention is represented by the formula:
• the compound of the present invention is represented by the formula:
• the compound of the present invention is represented by the formula:
• the compound of the present invention is represented by the formula:
• the compound of the present invention is represented by the formula:
• the compound of the present invention is represented by the formula:
• the compound of the present invention is represented by the formula:
• the compound of the present invention is represented by the formula:
• the compound of the present invention is represented by the formula:
• the compound of the present invention is represented by the formula:
• the compound of the present invention is represented by the formula:
• the compound of the present invention is represented by the formula:
• the compound of the present invention is represented by the formula:
• the compound of the present invention is represented by the formula:
• Cannabinoid receptor agonists according to the present invention can have anti-inflammatory activity and/or immunomodulatory activity and are useful in the treatment of various medical conditions including, e.g., rheumatoid arthritis, systemic lupus erythematosus, multiple sclerosis, glaucoma, diabetes, osteoporosis, renal ischemia, cerebral stroke, cerebral ischemia, nephritis, psoriasis, allergy, inflammatory disorders of the lungs and gastrointestinal tract such as Crohn's disease, and respiratory tract disorders such as reversible airway obstruction, asthma and bronchitis.
• various medical conditions including, e.g., rheumatoid arthritis, systemic lupus erythematosus, multiple sclerosis, glaucoma, diabetes, osteoporosis, renal ischemia, cerebral stroke, cerebral ischemia, nephritis, psoriasis, allergy, inflammatory disorders of the lungs
• Alkyl means an aliphatic hydrocarbon group which may be straight or branched and comprising 1 to about 20 carbon atoms in the chain. Preferred alkyl groups contain 1 to about 12 carbon atoms in the chain. More preferred alkyl groups contain 1 to about 6 carbon atoms in the chain. Branched alkyl means that one or more lower alkyl groups such as methyl, ethyl or propyl, are attached to a linear alkyl chain. "Lower alkyl” means a group having about 1 to about 6 carbon atoms in the chain which may be straight or branched. Preferred alkyl groups in the present invention are lower alkyl groups.
• Non-limiting examples of suitable alkyl groups include methyl, ethyl, n-propyl, isopropyl, n-butyl, t-butyl, n-pentyl, heptyl, nonyl, decyl, trifluoromethyl and cyclopropylmethyl.
• Alkenyl means an aliphatic hydrocarbon group containing at least one carbon- carbon double bond and which may be straight or branched and comprising 2 to 15 carbon atoms in the chain. Preferred alkenyl groups have 2 to 2 carbon atoms in the chain; and more preferably 2 to 6 carbon atoms in the chain. Branched alkyenyl means that one or more lower alkyl groups such as methyl, ethyl or propyl, are attached to a linear alkenyl chain. "Lower alkenyl” means 2 to 6 carbon atoms in the chain which may be straight or branched.
• alkenyl groups include ethenyl, propenyl, n-butenyl, 3-methylbut-2-enyl, and n-pentenyl.
• Halo means fluoro, chloro, bromo, or iodo groups. Preferred are fluoro, chloro or bromo, and more preferred are fluoro and chloro.
• Halogen means fluorine, chlorine, bromine, or iodine. Preferred are fluorine, chlorine or bromine, and more preferred are fluorine and chlorine.
• Haloalkyl or halogenated alkyl means alkyl having one or more halo atom substituents. Non-limiting examples include -CH 2 CI, -CHCI 2 , -CCI 3 , -CH 2 F, -CHF 2 , - CF 3 , -CH 2 -CH 2 CI, -CH 2 -CHCI 2 , and -CHCI-CH 2 CI.
• Heteroalkyl means straight or branched alkyl chain as defined above comprising 1 or more heteroatoms, which can be the same or different, and are independently selected from the group consisting of N, O and S.
• Aralkyl or “arylalkyl” means an aryl-alkyl- group in which the aryl and alkyl are as previously described. Preferred aralkyls comprise a lower alkyl group. Non-limiting examples of suitable aralkyl groups include benzyl, phenylethyl and naphthalenylmethyl. The aralkyl is linked to an adjacent moiety through the alkyl.
• Alkylaryl means an alkyl-aryl- group in which the alkyl and aryl are as previously described. Preferred alkylaryls comprise a lower alkyl group. Non-limiting examples of suitable alkylaryl groups include tolyl and xylyl. The alkylaryl is linked to an adjacent moiety through the aryl.
• Alkenyl means an aryl-alkenyl- group in which the aryl and alkenyl groups are as previously described. Preferred aralkenyls contain a lower alkenyl group. Non- limiting examples of suitable aralkenyl groups include phenylethenyl and naphthylethenyl. The aralkenyl is linked to an adjacent moiety through the alkenyl.
• Alkoxy means an alkyl-O- group in which the alkyl group is as previously described.
• suitable alkoxy groups include methoxy, ethoxy, n-propoxy and isopropoxy.
• the alkyl group is linked to an adjacent moiety through the ether oxygen.
• Aryloxy means an aryl-O- group in which the aryl group is as previously described.
• suitable aryloxy groups include phenoxy and naphthoxy. The aryl group is linked to an adjacent moiety through the ether oxygen.
• Alkyloxy means an aralkyl-O- group in which the aralkyl group is as previously described.
• suitable aralkyloxy groups include benzyloxy and naphthalenylmethoxy.
• the aralkyl group is linked to an adjacent moiety through the ether oxygen.
• Alkylamino means an -NH or -NH 3 + group in which one or more of the hydrogen atoms on the nitrogen is replaced by an alkyl group as defined above.
• Arylamino means an -NH 2 or -NH 3 + group in which one or more of the hydrogen atoms on the nitrogen is replaced by an aryl group as defined above.
• Alkylthio means an alkyl-S- group in which the alkyl group is as previously described.
• suitable alkylthio groups include methylthio, ethylthio and isopropylthio.
• the alkyl is linked to an adjacent moiety through the sulfur.
• Arylthio means an aryl-S- group in which the aryl group is as previously described.
• suitable arylthio groups include phenylthio and naphthylthio. The aryl is linked to an adjacent moiety through the sulfur.
• Aralkylthio means an aralkyl-S- group in which the aralkyl group is as previously described.
• a suitable aralkylthio group is benzylthio.
• the aralkyl is linked to an adjacent moiety through the sulfur.
• Alkoxycarbonyl means an alkyl-O-C(O)- group.
• suitable alkoxycarbonyl groups include methoxycarbonyl and ethoxycarbonyl. The alkoxy is linked to an adjacent moiety through the carbonyl.
• Aryloxycarbonyl means an aryl-O-C(O)- group.
• suitable aryloxycarbonyl groups include phenoxycarbonyl and naphthoxycarbonyl. The aryloxy is linked to an adjacent moiety through the carbonyl.
• Aralkoxycarbonyl means an aralkyl-O-C(O)- group.
• Non-limiting example of a suitable aralkoxycarbonyl group is benzyloxycarbonyl. The aralkoxy is linked to an adjacent moiety through the carbonyl.
• Alkylsulfonyl means an alkyl-S(0 2 )- group.
• the alkyl is linked to an adjacent moiety through the sulfonyl.
• the alkyl portion of the "alkylsulfonyl” is lower alkyl.
• Alkylsulfinyl means an alkyl-S(O)- group.
• the alkyl is linked to an adjacent moiety through the sulfinyl.
• the alkyl portion of the "alkylsulfinyl” is lower alkyl.
• Arylsulfonyl means an aryl-S(O )- group. The aryl is linked to an adjacent moiety through the sulfonyl.
• Aryl means an aromatic monocyclic or multicyclic ring system comprising 6 to 14 ring carbon atoms, preferably 6 to 10 ring carbon atoms.
• the aryl group can be optionally substituted with one or more "ring system substituents" which may be the same or different, and are as defined herein.
• suitable aryl groups include phenyl and naphthyl.
• Heteroaryl represents cyclic aromatic groups of 5 or 6 ring atoms or bicyclic groups of 11 to 12 ring atoms having 1 or 2 heteroatoms independently selected from O, S or N, said heteroatom(s) interrupting a carbocyclic ring structure and having a sufficient number of delocalized pi electrons to provide aromatic character, provided that the rings do not contain adjacent oxygen and/or sulfur atoms.
• Preferred heteroaryls contain 5 to 6 ring atoms.
• the “heteroaryl” can be optionally substituted by one or more "ring system substituents" which may be the same or different, and are as defined herein.
• the prefix aza, oxa or thia before the heteroaryl root name means that at least a nitrogen, oxygen or sulfur atom respectively, is present as a ring atom.
• Nitrogen atoms can form an N-oxide. All regioisomers are contemplated, e.g., 2- pyridyl, 3-pyridyl and 4-pyridyl.
• Useful 6-membered heteroaryl groups include pyridyl, pyrimidinyl, pyrazinyl, pyridazinyl and the like and the N-oxides thereof.
• Useful 5- membered heteroaryl rings include furyl, thienyl, pyrrolyl, thiazolyl, isothiazolyl, imidazolyl, pyrazolyl, isoxazolyl and the like.
• Useful bicyclic groups include benzo- fused ring systems derived from the heteroaryl groups named above, e.g. quinolyl, phthalazinyl, quinazolinyl, benzofuranyl, benzothienyl, indolyl and the like.
• Ring system substituent means a substituent attached to an aromatic or non- aromatic ring system which, for example, replaces an available hydrogen on the ring system.
• Ring system substituents may be the same or different, each being independently selected from the group consisting of aryl, heteroaryl, aralkyl, alkylamino, arylamino, alkylaryl, aralkenyl, heteroaralkyl, alkylheteroaryl, heteroaralkenyl, hydroxy, hydroxyalkyl, alkoxy, aryloxy, aralkoxy, aralkyloxy, acyl, aroyl, halo, nitro, cyano, carboxy, alkoxycarbonyl, aryloxycarbonyl, aralkoxycarbonyl, alkylsulfonyl, arylsulfonyl, heteroarylsulfonyl, alkylsulfinyl, arylsulfinyl, heteroarylsulfinyl, alkylthio, arylthio, heteroarylthio, aralkylthio, heteroaralkylthio,
• Cycloalkyl means a non-aromatic mono- or multicyclic fused ring system comprising 3 to 10 ring carbon atoms, preferably 3 to 7 ring carbon atoms, more preferably 3 to 6 ring carbon atoms.
• the cycloalkyl can be optionally substituted with one or more "ring system substituents" which may be the same or different, and are as defined above.
• suitable monocyclic cycloalkyls include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl and the like.
• suitable multicyclic cycloalkyls include 1 -decalinyl, norbornenyl, adamantyl and the like.
• Cycloheteroalkyl means a non-aromatic mono- or multicyclic fused ring system comprising 3 to 10 ring carbon atoms, preferably 3 to 7 ring carbon atoms, more preferably 3 to 6 ring carbon atoms, wherein the cycloheteroalkyl has 1 or 2 heteroatoms independently selected from O, S or N, said heteroatom(s) interrupting a carbocyclic ring structure provided that the rings do not contain adjacent oxygen and/or sulfur atoms.
• the cycloheteroalkyl can be optionally substituted with one or more "ring system substituents" which may be the same or different, and are as defined above.
• Non-limiting examples of cycloheteroaryl groups include piperidyl, pyrrolidinyl, piperazinyl, morpholinyl, thiomorpholinyl, thiazolidinyl, 1 ,3-dioxolanyl, 1 ,4- dioxanyl, tetrahydrofuranyl, tetrahydrothiophenyl, tetrahydrothiopyranyl, and the like
• optionally substituted means optional substitution with the specified groups, radicals or moieties.
• solvate as used herein means an aggregate that consists of a solute ion or molecule with one or more solvent molecules, for example, a hydrate containing such ions.
• composition and “formulation are intended to encompass a product comprising the specified ingredients, as well as any product which results, directly or indirectly, from combination of the specified ingredients.
• Principal includes mammals and other animals.
• mamal includes humans and other mammalian animals.
• pharmaceutically effective amount or “therapeutically effective amount” is intended to mean an amount of a therapeutic agent of the compound of formula I that will have an effect on a tissue, system, animal or patient that is being sought by the administrator (such as a researcher, doctor or veterinarian), which includes alleviation of the symptoms of the condition or disease being treated and the prevention, slowing or halting of progression of the disease or condition, for example, the inflammatory, immunomodulatory or respiratory diseases discussed herein.
• Prodrugs and solvates of the compounds of the invention are also contemplated within the scope of this invention.
• prodrug denotes a compound that is a drug precursor which, upon administration to a subject, undergoes chemical conversion by metabolic or chemical processes to yield a compound of formula I or a salt and/or solvate thereof.
• a discussion of prodrugs is provided in T. Higuchi and V. Stella, Pro-drugs as Novel Delivery Systems (1987) Volume 14 of the A.C.S. Symposium Series, and in Bioreversible Carriers in Drug Design, (1987) Edward B. Roche, ed., American Pharmaceutical Association and Pergamon Press, both of which are incorporated herein by reference thereto.
• the compounds of formula I can form salts, solvates and prodrugs which are also within the scope of this invention.
• Reference to a compound of formula I herein is understood to include reference to salts, solvates and prodrugs thereof, unless otherwise indicated.
• salt(s) denotes acidic salts formed with inorganic and/or organic acids, as well as basic salts formed with inorganic and/or organic bases.
• a compound of formula I contains both a basic moiety, such as, but not limited to, a pyridine or imidazole, and an acidic moiety, such as, but not limited to a carboxylic acid, zwitterions ("inner salts") may be formed and are included within the term “salt(s)” as used herein.
• Pharmaceutically acceptable (i.e., non-toxic, physiologically acceptable) salts are preferred, although other salts are also useful.
• Salts of the compounds of the formula I may be formed, for example, by reacting a compound of formula I with an amount of acid or base, such as an equivalent amount, in a medium such as one in which the salt precipitates or in an aqueous medium followed by lyophilization.
• Exemplary acid addition salts include acetates, adipates, alginates, ascorbates, aspartates, benzoates, benzenesulforiates, bisulfates, borates, butyrates, citrates, camphorates, camphorsulfonates, cyclopentanepropionates, digluconates, dodecylsulfates, ethanesulfonates, fumarates, glucoheptanoates, glycerophosphates, hemisulfates, heptanoates, hexanoates, hydrochlorides, hydrobromides, hydroiodides, 2-hydroxyethanesulfonates, lactates, maleates, methanesulfonates, 2- naphthalenesulfonates, nicotinates, nitrates, oxalates, pectinates, persulfates, 3- phenylpropionates, phosphate
• Exemplary basic salts include ammonium salts, alkali metal salts such as sodium, lithium, and potassium salts, alkaline earth metal salts such as calcium and magnesium salts, salts with organic bases (for example, organic amines) such as benzathines, dicyclohexylamines, hydrabamines (formed with N,N- bis(dehydroabietyl)ethylenediamine), N-methyl-D-glucamines, N-methyl-D- glucamides, t-butyl amines, and salts with amino acids such as arginine, lysine and the like.
• Basic nitrogen-containing groups may be quartemized with agents such as lower alkyl halides (e.g.
• dialkyl sulfates e.g. dimethyl, diethyl, dibutyl, and diamyl sulfates
• long chain halides e.g. decyl, lauryl, myristyl and
• All stereoisomers (for example, geometric isomers, optical isomers and the like) of the present compounds including those of the salts, solvates and prodrugs of the compounds as well as the salts and solvates of the prodrugs), such as those which may exist due to asymmetric carbons on various substituents, including enantiomeric forms (which may exist even in the absence of asymmetric carbons), rotameric forms, atropisomers, and diastereomeric forms, are contemplated within the scope of this invention.
• Individual stereoisomers of the compounds of the invention may, for example, be substantially free of other isomers, or may be admixed, for example, as racemates or with all other, or other selected, stereoisomers.
• the chiral centers of the present invention can have the S or R configuration as defined by the IUPAC 1974 Recommendations.
• the use of the terms “salt”, “solvate” “prodrug” and the like, is intended to equally apply to the salt, solvate and prodrug of enantiomers, stereoisomers, rotamers, tautomers, racemates or prodrugs of the inventive compounds.
• the present invention also relates to a pharmaceutical composition comprising one or more compounds of formula I of this invention.
• the pharmaceutical composition includes one or more pharmaceutically acceptable carriers.
• pharmaceutically acceptable carriers can be either solid or liquid.
• Solid form preparations include powders, tablets, dispersible granules, capsules, cachets and suppositories.
• the powders and tablets can be comprised of from about 5 to about 95 percent active ingredient.
• Suitable solid carriers are known in the art, e.g., magnesium carbonate, magnesium stearate, talc, sugar or lactose. Tablets, powders, cachets and capsules can be used as solid dosage forms suitable for oral administration. Examples of pharmaceutically acceptable carriers and methods of manufacture for various compositions can be found in A. Gennaro (ed.), Remington's Pharmaceutical Sciences, 18 th Edition, (1990), Mack Publishing Co., Easton, Pennsylvania.
• a low melting wax such as a mixture of fatty acid glycerides or cocoa butter is first melted, and the active ingredient is dispersed homogeneously therein as by stirring. The molten homogeneous mixture is then poured into convenient sized molds, allowed to cool and thereby solidify.
• Liquid form preparations include solutions, suspensions and emulsions.
• One example includes water or water-propylene glycol solutions for parenteral injection.
• Liquid form preparations may also include solutions for intranasal administration.
• Aerosol preparations suitable for inhalation may include solutions and solids in powder form, which may be in combination with a pharmaceutically acceptable carrier, such as an inert compressed gas.
• solid form preparations which are intended to be converted, shortly before use, to liquid form preparations for either oral or parenteral administration.
• liquid forms include solutions, suspensions and emulsions.
• the compounds of the invention may also be deliverable transdermally.
• the transdermal compositions can take the form of creams, lotions, aerosols and/or emulsions and can be included in a transdermal patch of the matrix or reservoir type as are conventional in the art for this purpose.
• Another aspect of the invention relates to a method of stimulating cannabinoid CB 2 receptors in a patient comprising administering to a patient a CB 2 receptor stimulating amount of one or more compounds of formula I.
• the daily dose of a compound of formula I for stimulating cannabinoid CB 2 receptors in a patient can range from about 0.001 mg/kg to about 100 mg/kg of body weight per day, preferably from about 0.001 mg/kg to about 50 mg/kg of body weight per day, more preferably from about 0.001 mg/kg to about 10 mg/kg of body weight per day.
• Another aspect of the invention relates to a method of treating cancer, inflammatory diseases, immunomodulatory diseases, or respiratory diseases comprising administering to a patient in need of such treatment one or more compounds of formula I.
• the amount of compound I administered in this aspect of the invention is a therapeutically effective amount.
• the daily dose of a compound of formula I for treatment of a disease or condition can range from about 0.001 mg/kg to about 100 mg/kg of body weight per day, preferably from about 0.001 mg/kg to about 50 mg/kg of body weight per day, more preferably from about 0.001 mg/kg to about 10 mg/kg of body weight per day.
• the dosage level can range from about 0.1 mg to about 700 mg of drug per day, given in a single dose or 2-4 divided doses.
• the exact dose is determined by the attending clinician and is dependent on the potency of the compound administered, the age, weight, condition and response of the patient.
• the compounds of the present invention can exhibit anti-inflammatory and/or immunomodulatory activity and are useful in the treatment of various medical conditions listed below. This utility is manifested as demonstrated by activity in the following assay.
• Test compounds were serially diluted in Diluent Buffer (50 mM Tris pH 7.1 , 1 mM EDTA, 3 mM MgCI 2 ⁇ 0.1% BSA, 10% DMSO, 0.36% methyl cellulose (Sigma M-6385)) from stocks prepared in 100% DMSO. Aliquots (10 ⁇ l) were transferred into 96-well microtiter plates.
• Diluent Buffer 50 mM Tris pH 7.1 , 1 mM EDTA, 3 mM MgCI 2 ⁇ 0.1% BSA, 10% DMSO, 0.36% methyl cellulose (Sigma M-6385)
• Binding Buffer 50 mM Tris pH 7.2, 1 mM EDTA, 3 mM MgCI 2j 0.1 % BSA.
• Each 100 ⁇ l reaction mixture contained 0.48 nM [ 3 H] CP-55,940, 8 ug membrane protein in binding buffer containing 1 % DMSO and 0.036 % methyl cellulose. Following incubation for 2 hours at room temperature, the reaction mixtures were
• Ki values ranging from about 0.1 nM to about 1 ⁇ M was observed.
• Preferred compounds 1-67 have Ki values ranging from about 0.1 nM to about 250 nM, preferably from about 0.1 to about 100nM, more preferably from about 0.1 to about 10 nM. It is contemplated that a compound of this invention can be useful in treating one or more of these diseases listed below.
• Non-limiting examples of the cancer, inflammatory diseases, immunomodulatory diseases, and respiratory diseases include diseases selected from the group consisting of cutaneous T cell lymphoma, rheumatoid arthritis, systemic lupus erythematosus, multiple sclerosis, glaucoma, diabetes, osteoporosis, renal ischemia, myocardial infarction, cerebral stroke, cerebral ischemia, nephritis, hepatitis, glomerulonephritis, cryptogenic fibrosing aveolitis, psoriasis, atopic dermatitis, vasculitis, allergy, seasonal allergic rhinitis, Crohn's disease, inflammatory bowel disease, reversible airway obstruction, adult respiratory distress syndrome, asthma, chronic obstructive pulmonary disease (COPD) and bronchitis.
• Compound I of the invention can be administered as a monotherapy. Additionally, compound I of the present invention can be co-administered or used in combination with one or more disease-modifying antirheumatic drugs (DMARDS) such as methotrexate, azathioptrine leflunomide, penicillamine, gold salts, mycophenolate mofetil, cyclophosphamide and other similar drugs.
• DARDS disease-modifying antirheumatic drugs
• non-steroidal anti-inflammatory drugs such as piroxicam, naproxen, indomethacin, ibuprofen and the like
• COX-2 selective inhibitors such as rofecoxib, which is in Vioxx® (from Merck & Company, Whitehouse Station, NJ) and celecoxib, which is in Celebrex® (from Pfizer Inc., New York, New York)
• COX-1 inhibitors such as Piroxicam, which is in Feldene® (from Pfizer Inc., New York, New York); immunosuppressives such as steroids, cyclosporine, Tacrolimus, rapamycin and the like
• biological response modifiers such as etanercept, which is in Enbrel® (from Wyeth-Ayerst, Philadelphia, PA), infliximab, which is in Remicade® (from Centocor, Inc., Malvern, PA), IL-1
• BRMs biological response modifiers
• drugs that the compounds of the invention can be co- administered or used in combination with include Anaprox, Arava, Arthrotec, Azulfidine, Aspirin, Cataflam, Celestone Soluspan, Clinoril, Cortone Acetate, Cuprimine, Daypro, Decadron, Depen, Depo-Medrol, Disalcid, Dolobid, Naprosyn, Gengraf, Hydrocortone, Imuran, Indocin, Lodine, Motrin, Myochrysine, Nalfon, Naprelan, Neoral, Orudis, Oruvail, Pediapred, Plaquenil, Prelone, Relafen, Solu- Medrol, Tolectin, Trilisate and Volataren. These include any formulation of the above named drugs.
• the compounds of the invention can be co-administered or used in combination with one or more additional agents, which may be the same or different, and are independently selected from the group consisting of Avonex® (Interferon B-1 a from Biogen), Betaseron® (Interferon B-1 b from Berlex) and Copaxone® (glatiramer acetate from Teva Neuroscience incorporated).
• additional agents which may be the same or different, and are independently selected from the group consisting of Avonex® (Interferon B-1 a from Biogen), Betaseron® (Interferon B-1 b from Berlex) and Copaxone® (glatiramer acetate from Teva Neuroscience incorporated).
• the active agents can be administered separately or in conjunction.
• the administration of one element can be prior to, concurrent to, or subsequent to the administration of the other agent, or other agents.
• the active agents should be given at the same time.
• kits for treating cancer, inflammatory diseases, immunomodulatory diseases, and respiratory diseases comprising in one or more containers an active compound for stimulating cannabinoid CB 2 receptors in a patient which comprises in one or more containers, one or more compounds according to claim 1 in one or more pharmaceutically acceptable carriers.
• the amount of compound I in the kit is a therapeutically effective amount.
• the daily dose of a compound of formula I for treatment of a disease or condition can range from about 0.001 mg/kg to about 100 mg/kg of body weight per day, preferably from about 0.001 mg/kg to about 50 mg/kg of body weight per day, more preferably from about 0.001 mg/kg to about 10 mg/kg of body weight per day.
• PG represents an N- protecting group.
• N-protecting groups suitable in the practice of the invention include allyl, methoxymethyl, benzyloxymethyl, CG 3 CO (where G is a halogen), benzyloxycarbonyl, trityl, pivaloyloxy methyl, tetrahydranyl, benzyl, di(p- methoxyphenyl)methyl, triphenylmethyl, (p-methoxyphenyl)diphenylmethyl, diphenylphosphinyl, benzenesulfenyl, methylcarbamate, 2-trimethylsilylethyl carbamate, 1-methyl-1-phenylethyl carbamate, t-butyl carbamate (“t-Boc”), cyclobutyl carbamate, 1-methylcyclobutyl carbamate, adamantyl carbamate, vinyl carbamate, allyl carbamate, cinnamyl carbamate, 8-quinolyl carbamate, 4,5-diphen
• step 1 trifluoroacetic anhydride is dissolved in a suitable inert solvent such as methylene chloride, chloroform, acetonitrile, dichloroethane and the like, and reacted with compound A, preferably at room temperature for 1-5 h, to form compound B.
• a suitable inert solvent such as methylene chloride, chloroform, acetonitrile, dichloroethane and the like
• step 2 compound B is dissolved in CISO 3 H between 0°C and room temperature, stirred at room temperature for about 72 h, and then poured into ice- H 2 O. A precipitate is formed containing compound C, which can be collected by filtration methods known in the art.
• Step 2 compound B-1 is dissolved in anhydrous THF and cooled to about -78° C and treated with nBuLi. The resulting lithiated species is quenched by bubbling S0 gas. The resulting mixture was warmed to room temperature and the precipitated lithium sulfonate salt was collected by filtration. The salt was redissolved in a 1 :1 mixture of water to methylene chloride and treated with chlorinating agent such as NCS. After workup, compound C was obtained.
• step 3 compound C is dissolved in a suitable solvent such as acetone and H 2 0. KF is added and it is stirred at room temperature. After work up, the product (compound D) can be purified via methods known in the art such as sgc or crystallization.
• a protected indole for example, N-Boc indole, used in step 4 is prepared by dissolving protecting group (PG) as defined above in a suitable inert solvent such as methylene chloride, dichloroethane, THF and DMF, and reacting with an indole derivative (compound I) in the presence of dimethylaminopyridine, trimethylamine, and (iPr) 2 NEt.
• PG protecting group
• a suitable inert solvent such as methylene chloride, dichloroethane, THF and DMF
• step 4 the protected indole (compound J) is dissolved in a suitable solvent such as EtOAc or ether, cooled in a dry ice/IPA bath and treated with n-BuLi. The resulting anion is trapped with compound D.
• a suitable solvent such as EtOAc or ether
• EtOAc or ether cooled in a dry ice/IPA bath
• n-BuLi n-BuLi
• step 5 compound E is dissolved in a suitable solvent such as dioxane, ethanol, methanol or THF and an alkali metal hydroxide or carbonate such as lithium hydroxide or potassium carbonate is added either as an aqueous solution or as a solid.
• a suitable solvent such as dioxane, ethanol, methanol or THF
• an alkali metal hydroxide or carbonate such as lithium hydroxide or potassium carbonate
• step 6 a combination of compound F and a tertiary amine base is dissolved in a suitable solvent such as methylene chloride or dioxane, at room temperature, cooled, and a suitable electrophile represented by R 1 -Y-LG is added, wherein LG is preferably CI or F.
• a suitable solvent such as methylene chloride or dioxane
• LG is preferably CI or F.
• the reaction mixture is stirred between -78 °C and room temperature for about 0.5 to about 48 h.
• the product (compound G) can be purified via sgc or crystallization.
• step 7 compound G is dissolved in a suitable inert solvent such as THF, methylene chloride, dichloroethane, or ether.
• Aqueous NaOH is used as a base.
• An electrophile represented by R 4 -L 2 -LG, wherein LG is preferably CI, is added and the reaction mixture is stirred in the presence of a phase-transfer catalyst (such as tetrabutyl ammonium hydrogen sulfate, methyl-n-butylammonium chloride, or benzyltriethylammonium hydroxide) between 0 °C and 100 °C for about 0.5 to about 48 h.
• the product (compound H) can be purified via sgc or crystallization.
• step 1 trifluoroacetic anhydride is dissolved in a suitable inert solvent, such as methylene chloride or dichloroethane, and reacted with a benzyl amine (compound G) at room temperature for 1-5 h.
• a suitable inert solvent such as methylene chloride or dichloroethane
• MsOH (2 eq) is added followed by DBDMH and the reaction mixture is stirred, preferably overnight at room temperature, and subjected to aqueous work up.
• the benzyl amide product (compound H) is recrystallized, preferably from a mixture of Et 2 0 and hexanes.
• step 2 compound H is dissolved in a suitable solvent such as THF or ether, cooled in a dry ice/acetone bath (-78 °C) and treated with n-BuLi. The dianion is then treated with a THF solution containing compound F. The resulting mixture is warmed to about rt and stirred for about 10 h.
• the product (compound I) can be purified by chromatography.
• step 3 compound I is dissolved in a suitable inert solvent such as THF, methylene chloride or dichloroethane, and reacted with Et 3 SiH and TFA between 0 °C and 100 °C for about 0.5 to about 48 h.
• the product (compound J) can be purified via chromatography.
• compound J is dissolved in a suitable solvent such as dioxane, ethanol, methanol or THF, and an alkali metal hydroxide or carbonate such as lithium hydroxide or potassium carbonate is added either as an aqueous solution or as a solid.
• a suitable solvent such as dioxane, ethanol, methanol or THF
• an alkali metal hydroxide or carbonate such as lithium hydroxide or potassium carbonate
• the reaction mixture is stirred at room temperature for 0.5-24 h.
• the product (compound K) can be purified via sgc or crystallization.
• a combination of compound K and a tertiary amine base is dissolved in a suitable solvent such as methylene chloride, THF, dichloroethane or dioxane, preferably at room temperature.
• the reaction mixture is cooled, and a suitable electrophile, represented by the formula R -Y-LG wherein LG is preferably CL or F, is added.
• the reaction mixture is stirred preferably at a temperature between -78 °C and room temperature for about 0.5 to about 48 h.
• the product (compound L) can be purified via sgc or crystallization.
• the aldehyde (compound F) used in step 2 is prepared by one of the following two procedures:
• a phase-transfer catalyst such as tetrabutyl ammonium hydrogen sulfate, methyl-n-butylammonium chloride, or benzyltriethylammonium hydroxide
• the product (compound F) can be purified via sgc or crystallization.
• step 1 compound A (the product of step 2 of Scheme II) is oxidized with PCC, in a suitable inert solvent such as methylene chloride, dichloroethane, or THF, to the carbonyl compound (B) by stirring preferably at rt for about 18 h.
• a suitable inert solvent such as methylene chloride, dichloroethane, or THF
• step 2 compound B is dissolved in a suitable solvent such as dioxane, ethanol, methanol or THF and an alkali metal hydroxide or carbonate such as lithium hydroxide or potassium carbonate is added either as an aqueous solution or as a solid.
• a suitable solvent such as dioxane, ethanol, methanol or THF
• an alkali metal hydroxide or carbonate such as lithium hydroxide or potassium carbonate
• step 3 a combination of compound C and a tertiary amine base is dissolved in a suitable solvent such as methylene chloride or dioxane, at room temperature, cooled, and a suitable electrophile represented by the formula R 1 -Y-LG, wherein LG is preferably CI, is added.
• a suitable solvent such as methylene chloride or dioxane
• LG is preferably CI
• the reaction mixture is stirred preferably between -78 °C and room temperature for about 0.5 to about 48 h.
• the product (compound D) can be purified via sgc or crystallization.
• step 1 the Bromo-furan (Compound A) (obtained from Aldrich Chemical Company, Inc. Milwaukee, Wl) is dissolved in THF or ether, cooled in a dry ice/IPA bath and treated with t-BuLi. The resulting anion is trapped with the sulfonyl fluoride compound (Compound i) which is prepared in step 3 of Scheme I.
• the product (compound B) can be purified via chromatography or crystallization.
• step 2 compound B is dissolved in THF or ether, and treated with a base such as t-BuLi at -78 °C to form a dianion, which is trapped with a suitable electrophile represented by R 4 -L 2 -LG, wherein LG is preferably CI or F.
• the reaction mixture is quenched with a suitable proton source such as aq NH 4 CI or phosphate buffer, then extracted with a suitable solvent such as EtOAc, ether or methylacetone.
• the product (compound C) can be purified via sgc or crystallization.
• step 3 compound C is dissolved in a suitable solvent, such as dioxane, ethanol, methanol or THF.
• a suitable solvent such as dioxane, ethanol, methanol or THF.
• An alkali metal hydroxide or carbonate such as lithium hydroxide, potassium carbonate, NaOH, KOH or sodium carbonate, is added either as an aqueous solution or as a solid.
• the reaction mixture is stirred preferably at room temperature for 0.5-24 h.
• the product (compound D) can be purified via sgc or crystallization.
• step 4 a combination of compound D and a tertiary amine base is dissolved in a suitable solvent, such as methylene chloride, THF, dichloroethane or dioxane, at room temperature, cooled, and a suitable electrophile represented by the formula R 1 - Y-LG is added, wherein LG is preferably CI.
• a suitable solvent such as methylene chloride, THF, dichloroethane or dioxane
• the reaction mixture is stirred preferably between -78 °C and room temperature for about 0.5 to about 48 h.
• the product (Compound E) can be purified via sgc or crystallization.
• step 1 the acyl chloride (compound A) and N-methoxyl-N-methylamine HCI (compound B) which is commercially available are dissolved in a suitable solvent, such as THF, dioxane or CH 2 CI 2 , and treated with a base such as triethylamine, (iPr) 2 NEt and/or DMAP, preferably at room temperature for 0.5-24 h.
• a suitable solvent such as THF, dioxane or CH 2 CI 2
• a base such as triethylamine, (iPr) 2 NEt and/or DMAP, preferably at room temperature for 0.5-24 h.
• the product (compound C) can be purified via sgc or crystallization.
• step 2 compound i, which is prepared in step 1 of Scheme II, is dissolved in THF, cooled in a dry ice/I PA bath and treated with n-BuLi. The resulting anion is trapped with compound C.
• the product (compound D) can be purified via chromatography or crystallization.
• step 3 the compound D is dissolved in a suitable solvent such as dioxane, ethanol, methanol or THF and an alkali metal hydroxide or carbonate such as lithium hydroxide or potassium carbonate is added either as an aqueous solution or as a solid. The reaction mixture is stirred at room temperature for 0.5-24 h.
• the product (compound E) can be purified via sgc or crystallization.
• step 4 a combination of compound E and a tertiary amine base is dissolved in a suitable solvent such as methylene chloride or dioxane, at room temperature, cooled, and a suitable electrophile represented by R 1 -Y-LG, wherein LG is preferably CI.
• a suitable solvent such as methylene chloride or dioxane
• LG is preferably CI.
• the reaction mixture is stirred between -78 °C and room temperature for about 0.5 to about 48 h.
• the product (compound F) can be purified via sgc or crystallization.
• step 5 compound F is dissolved in a suitable solvent such as methanol.
• NaBH 4 is added at 0 °C.
• the reaction mixture is then stirred between -78 °C and room temperature for about 0.5 to about 48 h.
• the product (compound G) can be purified via sgc or crystallization.
• step 6 compound G is dissolved in a suitable solvent such as methylene chloride, and treated with Et 3 SiH and TFA at room temperature for 0.5 to 120 h.
• the product (compound H) can be purified via sgc or crystallization.
• step 1 2,4-dibromophenol (Compound A) (obtained from Aldrich Chemical Company, Inc. Milwaukee, Wl) and dibromoethane are dissolved in a suitable solvent, such as methylene chloride, benzene, dichloroethane or toluene, and an aqueous base such as NaOH, KOH or LiOH.
• a suitable solvent such as methylene chloride, benzene, dichloroethane or toluene
• an aqueous base such as NaOH, KOH or LiOH.
• the reaction mixture is stirred in the presence of a phase-transfer catalyst (such as tetrabutyl ammonium hydrogen sulfate, methyl-n- butylammonium chloride, or benzyltriethylammonium hydroxide) between 0 °C and 100 °C for about 0.5 to about 48 h.
• the product (compound B) can be purified via sgc
• step 2 compound B is dissolved in THF or ether, preferably between -78 °C to -100 °C. After stirring, the reaction mixture is treated with another portion of base at preferably between -78 °C to -100 °C. The resulting anion is trapped with compound (i) prepared in step 3 of Scheme I.
• the product (compound C) can be purified via chromatography or crystallization.
• step 3 the product of step 2 (compound C) is dissolved in THF or ether, and treated with a base such as n-BuLi, preferably between -78 °C and -100 °C to form a dianion, which is trapped with a suitable electrophile represented by R 4 -L 2 -LG.
• the reaction mixture is quenched with a suitable proton source such as aq NH CI or phosphate buffer, then extracted with EtOAc or ether.
• the product (compound D) can be purified via sgc or crystallization.
• step 4 the product of step 3 (compound D) is dissolved in a suitable solvent such as dioxane, ethanol, methanol or THF and an alkali metal hydroxide or carbonate such as lithium hydroxide or potassium carbonate is added either as an aqueous solution or as a solid.
• a suitable solvent such as dioxane, ethanol, methanol or THF
• an alkali metal hydroxide or carbonate such as lithium hydroxide or potassium carbonate is added either as an aqueous solution or as a solid.
• the reaction mixture is stirred preferably at room temperature for 0.5-24 h.
• the product (compound E) can be purified via sgc or crystallization.
• step 5 a combination of the product of step 4 (compound E) and a tertiary amine base, such as triethylamine or (iPr) 2 NEt, is dissolved in a suitable solvent such as methylene chloride, dichloroethane or dioxane, at room temperature, cooled, and a suitable electrophile represented by R 1 -Y-LG is added wherein LG preferably represents CI.
• a suitable solvent such as methylene chloride, dichloroethane or dioxane
• R 1 -Y-LG a suitable electrophile represented by R 1 -Y-LG is added wherein LG preferably represents CI.
• the reaction mixture is stirred preferably between -78 °C and room temperature for about 0.5 to about 48 h.
• the product (Compound F) can be purified via sgc or crystallization.
• Step 2 Compound B from step 1 was dissolved in 100 ml of chlorosulfonic acid at 0 °C and stirred for 72 h allowing the temperature to rise to about room temperature. The reaction mixture was poured into a 500 ml ice-H 2 O mixture and stirred vigorously for 2 h. The product (compound C) was collected by filtration, recrystallized from ether, and dried under reduced pressure to yield 52 g of pure C.
• compound B-1 (10 g, 33.8 mmol) (Compound E from example 4) was dissolved in dry THF (100 mL) and cooled to -78 °C. A solution of n-butyl lithium (2.0 M in hexanes, 34 mL, 68.0 mmol) was added and the reaction mixture was stirred for 20 minutes. A solution of SO 2 in Et 2 O (300 mL) was added and the reaction was stirred for one hour. The reaction mixture was slowly warmed up to room temperature and stirred overnight. Solid was collected by suction filtration and then washed with Et 2 0.
• Step 5 In a flame dried flask under N 2 blanket, compound F (2.0 g, 9.2 mmol) was dissolved in dry THF (20 mL) and cooled to -78 °C. A solution of n-butyl lithium (1.7 M in hexanes, 5.9 mL, 10.0 mmol) was added and the reaction mixture was stirred for 45 min. Compound D (0.5 g, 1.7 mmol) in THF (1.5 mL) was added and the reaction mixture was stirred at -78 °C for 1 h.
• Step 6 (90 mg, 0.23 mmol) of compound G was dissolved in methanol (1.5 mL) at room temperature. LiOH (1.0 M, 0.90 mL, 0.90 mmol) was added and the mixture was stirred at room temperature overnight. The solvent was removed and CH 2 CI (15 mL) and brine (15 mL) were added and the layers were separated. The aqueous layer was extracted with additional CH 2 CI 2 (15 mL) and the combined organic layers were dried over Na 2 SO 4 and concentrated to dryness to give compound H. This material was used without further purification.
• Step 7 Compound H from Step 6 was dissolved in CH 2 CI 2 (10 mL) and cooled to 0 °C.
• Step 8 Compound I (64 mg, 0.17 mmol) was dissolved in CH 2 CI 2 (5 mL). NaOH
• Step 2 in Example I involves the following reaction:
• Step 1 Compound A (prepared from step 5 of Example 1) was dissolved in THF
• Step 2 Compound B (7.0 mg, 17 mmol) was dissolved in CH 2 CI 2 (10 mL) and cooled to 0 °C. Methanesulfonyl chloride (2.0 mg, 17 mmol) was added followed by addition of triethylamine (2.0 mg, 20 mmol). The reaction mixture was slowly warmed up to room temperature and stirred for overnight. The solvent was removed and the crude product was purified via PTLC (50% EtOAc/hexanes) to give 3.0 mg (36%) of compound C.
• Step 1 Compound A (46 mg, 0.11 mmol) was dissolved in CH 2 CI 2 (10 mL) at room temperature. 2-Fluorobenzoyl chloride (22 mg, 0.14 mmol) was added followed by addition of triethylamine (15 mg, 0.15 mmol) and DMAP (cat.). The reaction mixture was stirred at room temperature for 2 h. Another portion of 2-fluorobenzoyl chloride (22 mg, 0.14 mmol) was added to the reaction mixture, which was then stirred for an additional 4 h. The reaction mixture was washed with aqueous NaCl. The organic layer was dried over Na 2 S0 4 and then concentrated to dryness. The crude product was purified via PTLC (50% EtOAc/hexanes) to give 7.0 mg (12%) of compound B.
• Example IV 2-Fluorobenzoyl chloride
• Step 1 In a flame dried flask under N 2 blanket, indole (5.0 g, 43 mmol) (obtained from Aldrich Chemical Company, Inc. Milwaukee, Wl) was dissolved in dry THF (40 mL) and cooled to -78 °C. A solution of n-butyl lithium (2.5 M in hexanes, 18 mL, 45 mmol) was added and the reaction mixture was stirred for 1 h while being warmed up to 0 °C. The reaction suspension was cooled to -78 °C and a solution of p- methoxyphenyl sulfonyl chloride (9.7 g, 44 mmol) in THF (20 mL) was added dropwise.
• the reaction mixture was slowly warmed up to room temperature and stirred for 2 days.
• the mixture was poured into NaHCO 3 (2% aq, 120 mL) and extracted with diethyl ether (4 x 50 mL).
• the combined organic layers were washed with NaHC0 3 (2% aq, 50 mL), H 2 0 (4 x 75 mL) and brine (2 x 50 mL) respectively. It was dried over Na 2 SO 4 , and concentrated to give 12.4 g of crude material. This material was further washed with hexanes to give 11.8 g (96%) of pure compound B.
• Step 2 In a flame dried flask under N 2 blanket, diisopropylamine (0.4 g, 3.9 mmol) was dissolved in dry THF (8 mL) and cooled to -78 °C. A solution of n-butyl lithium (2.5 M in hexanes, 1.46 mL, 3.65 mmol) was added and the reaction mixture was stirred for 0.5 h to form LDA. To this solution was added dropwise a solution of compound B (1.0 g, 3.48 mmol) in THF (10 mL).
• the mixture was stirred for 1.5 h at below -70 °C and then allowed to warm slowly to 5 °C over 1.5 h.
• the reaction mixture was recooled to -78 °C, and DMF (0.50 g, 6.8 mmol) was added.
• the reaction mixture was warmed up to room temperature slowly.
• the reaction mixture was poured into HCI (10 % aq, 150 mL), and extracted with CH 2 CI 2 (3 x 100 mL). The combined organic layer was washed with brine, then dried over Na 2 S0 , and concentrated to dryness.
• the crude product was purified via sgc (10% EtOAc/hexanes) to give 367 mg (33%) of compound C.
• Step 3 TFAA (67 mL, 0.474 mol) was dissolved in CH 2 CI 2 (300 mL) and cooled in an ice water bath. A solution of (S)- ⁇ methyl benzyl amine (56.4g, 0.465 mol) dissolved in CH 2 CI 2 (100 mL) (obtained from Aldrich Chemical Company, Inc. Milwaukee, Wl) was added and the ice bath was removed. The reaction mixture was stirred at rt for 3 h. The reaction mixture was cooled in an ice bath and MsOH (80 mL, 1.23 mol) was added followed by DBDMH (65g, 0.227 mol).
• Step 4 In a flame dried flask under N 2 blanket, compound E (0.28 g, 0.94 mmol) was dissolved in dry THF (20 mL) and cooled to -78 °C. A solution of methyl lithium (1.4 M in hexanes, 0.74 mL, 1.04 mmol) was added and the reaction mixture was stirred for 15 min. A solution of n-butyl lithium (2.5 M in hexanes, 0.41 mL, 1.03 mmol) was added and the reaction mixture was stirred for 30 min. Compound C (0.36 g, 1.13 mmol) in THF (3 mL) was added to the reaction mixture at -78 °C and was slowly warmed to -10 °C.
• Triethylsilane (440 mg, 3.825 mmol) was added followed by addition of TFA (47 mg, 0.42 mmol). The reaction mixture was then stirred at room temperature overnight followed by refluxing for 2-3 h. The reaction was quenched by addition of NaHC0 3 (sat. aq). It was extracted with CH 2 CI 2 (2 x 20 mL).
• Step 7 Compound H (36.5 mg, 69 mmol) was dissolved in methanol (10 mL) at room temperature. NaOH (2.0 M, 0.52 mL, 1.04 mmol) was added and the mixture was stirred at room temperature for 3 days. The solvent was removed and CH 2 CI 2 (15 mL) and brine (15 mL) was added and the layers were separated. The aqueous layer was extracted with additional CH 2 CI 2 (15 mL) and the combined organic layers were dried over Na 2 S0 4 and concentrated to give 21.6 mg (72%) of compound I. Step 8. Compound I (21.6 mg, 50 mmol) was dissolved in CH 2 CI 2 (10 mL) and cooled to 0 °C.
• Step 1 In a flame dried flask under N 2 blanket, 3-bromofuran (1.3 g, 9.0 mmol) was dissolved in dry THF (50 mL) and cooled to -78 °C. A solution of n-butyl lithium (1.6 M in hexanes, 5.6 mL, 9.0 mmol) was added and the reaction mixture was stirred for 15 min. The sulfonyl fluoride compound i (prepared in step 3 of Example I) (0.90 g, 3.0 mmol) in THF (3 mL) was added to the reaction mixture at -78 °C and stirred for 3 h.
• Step 2 In a flame dried flask under N 2 blanket, compound B (440 mg, 1.3 mmol) was dissolved in dry THF (20 mL) and cooled to -78 °C. A solution of -butyl lithium (1.5 M in hexanes, 1.7 mL, 2.5 mmol) was added and the reaction mixture was stirred for 15 min. 2-fluorophenyl disulfide (0.32 g, 1.3 mmol) in THF (5 mL) was added and the reaction mixture was stirred at -78 °C for 1 h before slowly warming up to 0 °C. The reaction mixture was then quenched with saturated aqueous NH 4 CI (50 mL). Ethyl acetate (100 mL) was added and the layers were separated. The organic layer was washed with brine, then dried over Na 2 S0 4 , and concentrated to dryness. The crude product of compound C was used without further purification.
• Step 3 Compound C (270 mg, 0.57 mmol) was dissolved in CH 2 CI 2 (15 mL). mCPBA(210 mg, 57-86%, 0.69 mmol) was added and the solution was stirred at room temperature for 1 h. The reaction mixture was diluted with CH 2 CI 2 (50 mL), washed with NaHS0 3 (40 mL) and NaHCO 3 (50 mL). The organic layers were combined and dried over Na 2 S0 4 and concentrated. The crude material was purified via sgc (50% EtOAC / Hexanes) to give 120 mg (43%) of compound D. Step 4: Compound D (70 mg, 0.14 mmol) was dissolved in CH 2 CI 2 (10 mL).
• Step 6 The crude product of compound F was dissolved in CH 2 CI 2 (5 mL) and cooled to -78 °C. Et 3 N (15 mg, 0.15 mmol) was added followed by the addition of trifluoromethanesulfonic anhydride (34 mg, 0.12 mmol). The reaction mixture was stirred for 2h before warming up to 0 °C.
• Step 1 To a suspension of N,0-dimethylhydroxylamine (compound B), hydrogen chloride (0.80 g, 8.2 mmol) in methylene chloride (35 mL) was added Et 3 N (1.0 g, 9.8 mmol). It was stirred at room temperature for 10 min. To the resulting solution was added a solution of 2-(4-chlorophenoxy) pyridine (compound A) (2.0 g, 7.5 mmoL) (obtained from Maybridge Ltd. UK) in methylene chloride (40 mL) dropwise. The reaction mixture was stirred at room temperature overnight and H 2 0 (40 mL) was added. The organic layer was separated and washed with brine and dried over Na 2 S0 and concentrated.
• compound A 2-(4-chlorophenoxy) pyridine
• Step 2 In a flame dried flask under N 2 blanket, compound i (prepared in step 3 of Example IV) (0.1 g, 0.34 mmol) was dissolved in dry THF (20 mL) and cooled to -78 °C. A solution of methyl lithium (1.4 M in hexanes, 0.27 mL, 0.37 mmol) was added and the reaction mixture was stirred for 15 min. A solution of n-butyl lithium (1.6 M in hexanes, 0.23 mL, 0.37 mmol) was added and the reaction mixture was stirred for 30 min.
• Step 4 The crude product of compound E was dissolved in CH 2 CI 2 (10 mL) and cooled to 0 °C. Methanesulfonyl chloride (33 mg, 0.28 mmol) was added followed by addition of pyridine (56 mg, 0.7 mmol). The reaction mixture was slowly warmed up to room temperature and stirred overnight. Brine (15 mL) was added and the product was extracted with CH 2 CI 2 (50 mL). The organic layer was dried over Na 2 S0 4 and concentrated to dryness. The crude product was purified via PTLC (50% EtOAc/hexanes) to give 24 mg of compound F as a colorless oil.
• Step 5 A solution of compound F (0.28 g, 0.64 mmol) in EtOH (34 mL) and H 2 0
• Step 6 To a solution of compound F (0.28 g, 0.65 mmol) in MeOH (20 mL) was added NaBH 4 (0.2 g, 5.2 mmol) at 0 °C and stirred for 0.5 h. H 2 0 was added to quench the reaction. MeOH was removed by vacuum. The mixture was extracted with methylene chloride (3 x 25 mL) and the combined organic layers were washed with brine and dried over Na 2 S0 4 . Compound H was obtained by removing the organic solvent. It gave 0.28 g of compound H.
• Step 7 To a solution of compound H (0.28 g, 0.64 mmol) in methylene chloride
• Step 1 To a suspension of N,0-dimethylhydroxylamine hydrogen chloride
• Step 2 In a flame dried flask under N 2 blanket, compound i (prepared in step 3 of Example IV) (0.1 g, 0.34 mmol) was dissolved in dry THF (20 mL) and cooled to -78 °C. A solution of methyl lithium (1.4 M in hexanes, 0.27 mL, 0.37 mmol) was added and the reaction mixture was stirred for 15 min. A solution of n-butyl lithium (1.6 M in hexanes, 0.23 mL, 0.37 mmol) was added and the reaction mixture was stirred for 30 min.
• Step 3 Compound D (93 mg, 0.2 mmol) was dissolved in dioxane (3 mL) at room temperature. LiOH (1.0 M, 1.2 mL, 1.2 mmol) was added and the mixture was stirred at room temperature overnight. The solvent was removed and CH 2 CI (15 mL) and brine (15 mL) were added and the layers were separated. The aqueous layer was extracted with additional CH 2 CI 2 (15 mL) and the combined organic layers were dried over Na 2 S0 4 and concentrated to dryness to give compound E.
• Step 4 The crude product of compound E was dissolved in CH 2 CI 2 (10 mL) and cooled to 0 °C.
• Step 1 A mixture of 2,4-dibromophenol (compound A) (5g, 0.02 mol), 1 ,2 dibromoethane (37 mL, 0.4 mol) (obtained from Aldrich Chemical Company, Inc. Milwaukee, Wl), and aq. NaOH (14 ml, 3N) Bu 4 N + HS0 4 " (0.34 g, 1 mmol) was stirred vigorously at 70 °C for 10 h. The mixture was cooled to room temperature. CH 2 CI 2 (100 mL) was added. The organic layer was washed with NaOH(1 N), HCI(1 N), water and brine, respectively. The organic layer was dried over Na 2 S0 4 , and concentrated to dryness. The crude product was purified by recrystallization from ethe ⁇ hexane to give 3.2 g (45%) of pure compound B.
• Step 2 In a flame dried flask under N 2 blanket, compound B (2.6 g, 7.2 mmol) was dissolved in dry THF (40 mL) and cooled to -100 °C. A solution of n-butyl lithium (2.5 M in hexanes, 3.17 mL, 7.9 mmol) was added and the reaction mixture was stirred for 30 min. The reaction mixture was warmed to
• Step 3 In a flame dried flask under N 2 blanket, compound C (1.1 g, 2.8 mmol) was dissolved in dry THF (20 mL) and cooled to -78 °C. A solution of n-butyl lithium (1.7 M in hexanes, 3.6 mL, 6.0 mmol) was added and the reaction mixture was stirred for 45 min. Pyridine disulfide (0.6 g, 3.0 mmol) in THF (5 mL) was added and the reaction mixture was stirred at -78 °C for 2 h before slowly warming up to room temperature.
• Step 4 Compound D (670 mg, 1.3 mmol) was dissolved in CH CI 2 (50 mL) with
• Step 1 Commercially available sulfonyl chloride (compound A) (1g, 3mmol) (obtained from Lancaster Synthesis Ltd., UK) in 20 mL acetone was treated with excess potassium fluoride (1.4g, 24 mmol) in a 20 mL aqueous solution. The reaction was stirred for 3 h and then diluted with 100 mL ethylacetate. The organics were washed with 2 X 50 mL water, dried over MgS0 4 and concentrated to yeild pure solid sulfonyl fluoride (compound B) ( 0.8g).
• Step 2 Bromophenethyamine trifluoroacetamide (compound i prepared in step 3 of example IV) (0.5 g, 18 mmol) in 25 mL anhydrous THF was treated with methyllithium (2 mmol) at -78°C. After stirring the mixture for 15 min, nBuLi (2mmol) was added. The reaction mixture was stirred for 0.5 h and then sulfonyl fluoride (compound B) (0.5 g) was added in a 10 mL THF solution. The reaction mixture was stirred for 3 h, and at the same time, the temparture was allowed to rise to 0°C. The reaction mixture was then quenched with aqueous ammonium chloride.

Landscapes

• Chemical & Material Sciences (AREA)
• Organic Chemistry (AREA)
• Health & Medical Sciences (AREA)
• Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
• Public Health (AREA)
• General Health & Medical Sciences (AREA)
• Veterinary Medicine (AREA)
• Chemical Kinetics & Catalysis (AREA)
• General Chemical & Material Sciences (AREA)
• Medicinal Chemistry (AREA)
• Animal Behavior & Ethology (AREA)
• Pharmacology & Pharmacy (AREA)
• Life Sciences & Earth Sciences (AREA)
• Engineering & Computer Science (AREA)
• Bioinformatics & Cheminformatics (AREA)
• Pulmonology (AREA)
• Rheumatology (AREA)
• Diabetes (AREA)
• Physical Education & Sports Medicine (AREA)
• Orthopedic Medicine & Surgery (AREA)
• Urology & Nephrology (AREA)
• Dermatology (AREA)
• Ophthalmology & Optometry (AREA)
• Heart & Thoracic Surgery (AREA)
• Vascular Medicine (AREA)
• Immunology (AREA)
• Biomedical Technology (AREA)
• Neurology (AREA)
• Neurosurgery (AREA)
• Pain & Pain Management (AREA)
• Obesity (AREA)
• Cardiology (AREA)
• Emergency Medicine (AREA)
• Gastroenterology & Hepatology (AREA)
• Otolaryngology (AREA)
• Endocrinology (AREA)
• Hematology (AREA)
• Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
• Pyridine Compounds (AREA)
• Plural Heterocyclic Compounds (AREA)

Priority Applications (6)

Applications Claiming Priority (2)

Publications (1)

Family

ID=30000466

Family Applications (1)

Country Status (14)

Cited By (13)

Families Citing this family (20)

Citations (4)

Family Cites Families (52)

• 2003
  • 2003-06-17 AU AU2003243637A patent/AU2003243637A1/en not_active Abandoned
  • 2003-06-17 PE PE2003000610A patent/PE20040574A1/es not_active Application Discontinuation
  • 2003-06-17 ES ES03761108T patent/ES2303599T3/es not_active Expired - Lifetime
  • 2003-06-17 EP EP03761108A patent/EP1539693B9/en not_active Expired - Lifetime
  • 2003-06-17 CN CN038144417A patent/CN1662496A/zh active Pending
  • 2003-06-17 AT AT03761108T patent/ATE388937T1/de not_active IP Right Cessation
  • 2003-06-17 CA CA002487346A patent/CA2487346A1/en not_active Abandoned
  • 2003-06-17 DE DE60319714T patent/DE60319714T2/de not_active Expired - Lifetime
  • 2003-06-17 US US10/464,174 patent/US7217732B2/en not_active Expired - Fee Related
  • 2003-06-17 JP JP2004515897A patent/JP2005533809A/ja active Pending
  • 2003-06-17 MX MXPA04012704A patent/MXPA04012704A/es unknown
  • 2003-06-17 WO PCT/US2003/019245 patent/WO2004000807A1/en not_active Ceased
  • 2003-06-18 TW TW092116534A patent/TW200405892A/zh unknown
  • 2003-06-18 AR ARP030102152A patent/AR039698A1/es unknown

Patent Citations (4)

Also Published As

Similar Documents

Legal Events