WO2003083475A1 - Gel auquel est fixee une substance biologique et microreseau comprenant ledit gel - Google Patents

Gel auquel est fixee une substance biologique et microreseau comprenant ledit gel Download PDF

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Publication number
WO2003083475A1
WO2003083475A1 PCT/JP2003/004274 JP0304274W WO03083475A1 WO 2003083475 A1 WO2003083475 A1 WO 2003083475A1 JP 0304274 W JP0304274 W JP 0304274W WO 03083475 A1 WO03083475 A1 WO 03083475A1
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Prior art keywords
gel
immobilized
microarray
hollow
tubular body
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PCT/JP2003/004274
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English (en)
Japanese (ja)
Inventor
Chiaki Nagahama
Chiho Itou
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Mitsubishi Rayon Co., Ltd.
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Application filed by Mitsubishi Rayon Co., Ltd. filed Critical Mitsubishi Rayon Co., Ltd.
Priority to US10/508,946 priority Critical patent/US20060084060A1/en
Priority to JP2003580857A priority patent/JP3654894B2/ja
Priority to CA2481323A priority patent/CA2481323C/fr
Priority to AU2003236359A priority patent/AU2003236359A1/en
Priority to EP03745212A priority patent/EP1491888B1/fr
Priority to IL16436603A priority patent/IL164366A0/xx
Priority to DE60323615T priority patent/DE60323615D1/de
Priority to KR1020047015783A priority patent/KR100989196B1/ko
Publication of WO2003083475A1 publication Critical patent/WO2003083475A1/fr
Priority to US12/014,251 priority patent/US20090023601A1/en
Priority to US13/098,613 priority patent/US8802369B2/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N37/00—Details not covered by any other group of this subclass
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B01J2219/00315—Microtiter plates
    • B01J2219/00317—Microwell devices, i.e. having large numbers of wells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00513—Essentially linear supports
    • B01J2219/0052—Essentially linear supports in the shape of elongated tubes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00513—Essentially linear supports
    • B01J2219/00524—Essentially linear supports in the shape of fiber bundles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/0054—Means for coding or tagging the apparatus or the reagents
    • B01J2219/00572—Chemical means
    • B01J2219/00576—Chemical means fluorophore
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00639—Making arrays on substantially continuous surfaces the compounds being trapped in or bound to a porous medium
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00639—Making arrays on substantially continuous surfaces the compounds being trapped in or bound to a porous medium
    • B01J2219/00641—Making arrays on substantially continuous surfaces the compounds being trapped in or bound to a porous medium the porous medium being continuous, e.g. porous oxide substrates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00639—Making arrays on substantially continuous surfaces the compounds being trapped in or bound to a porous medium
    • B01J2219/00644—Making arrays on substantially continuous surfaces the compounds being trapped in or bound to a porous medium the porous medium being present in discrete locations, e.g. gel pads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00673—Slice arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/13—Hollow or container type article [e.g., tube, vase, etc.]
    • Y10T428/1352—Polymer or resin containing [i.e., natural or synthetic]

Definitions

  • the present invention relates to a biomaterial-immobilized gel and a biomaterial-immobilized gel microarray using the same.
  • capillary gel electrophoresis As a means of gene analysis, gel electrophoresis has been used for a long time. In recent years, capillary gel electrophoresis has been developed to separate and analyze very small amounts of biological samples in a short time. In capillary gel electrophoresis, a glass capillary filled with a hydrogel such as acrylamide is used.
  • a microarray in which a plurality of probes capable of capturing DNA, protein, or the like are held is used.
  • many types of microarrays utilizing gels are known. Among them, as a microarray using a gel for fixing the capture probe, for example, a plurality of grooves or holes are formed in a base such as a resin plate, and the inside of the grooves or holes is filled with a gel containing DNA. (See Japanese Patent Application Laid-Open No.
  • the microarray to which the capture probe is immobilized is used for detection of a specific base sequence by performing a hybridization operation together with the sample.
  • Hybrid detection is performed by a known means capable of specifically recognizing the hybrid, for example, fluorescence detection.
  • the distribution of the fluorescence intensities in the compartments is uniform, and the total sum of the fluorescence intensities in each compartment is a higher value, that is, a higher hybridization.
  • the purpose of the present invention is to obtain a gel composition capable of obtaining the efficiency of the dilution.
  • the present inventors have conducted intensive studies to solve the above problems, and as a result, by immobilizing the capture probe on a gel satisfying the following properties, the distribution of the fluorescence intensity in the compartment becomes uniform, In addition, the present inventors have found that the fluorescence intensity of the compartment is high, that is, high hybridization efficiency can be obtained, and have reached the present invention.
  • ⁇ , ⁇ -dimethylacrylamide is used in an amount of 2 to 7% by mass / hour.
  • This is a bio-related substance-immobilized gel in which the bio-related substance is immobilized on the containing gel.
  • the present invention is a biomaterial-immobilized gel in which a biomaterial is immobilized on a gel having the following composition.
  • examples of the bio-related substance include nucleic acids.
  • examples of the cross-linking agent include a polyfunctional monomer having at least two ethylenically unsaturated bonds, for example, methylene bisacrylamide.
  • the present invention provides 2 to 7 mass of N, N-dimethylacrylamide. /.
  • a method for producing a biomaterial-immobilized gel, comprising fixing a biomaterial to a gel containing the biomaterial.
  • the gel is preferably obtained by reacting 2 to 7% by mass of ⁇ , ⁇ -dimethylacrylamide in the presence of 0.1 to 1.5% by mass of a crosslinking agent.
  • the present invention is a gel-filled hollow tubular body in which the bio-related substance-immobilized gel is filled in a hollow portion of a hollow tubular body.
  • the hollow tubular body include a hollow fiber.
  • the present invention provides a method for producing a gel microarray having a biomaterial immobilized thereon, wherein a plurality of the gel-filled hollow tubular bodies are bundled, and the bundle is cut in a direction intersecting the longitudinal direction of the tubular body. It is.
  • the present invention is a method for producing a gel microarray immobilized with a biological substance, which comprises the following steps.
  • the present invention is a bio-related substance-immobilized gel microarray in which the bio-related substance-immobilized gel is arranged in a plurality of sections.
  • it is preferable surface area of each compartment is 10_ 6 m 2 or less.
  • the gel microarray for immobilizing a biological substance a gel microarray in which a section is formed by a groove or a through hole can be used.
  • the present invention provides a bio-related substance immobilization obtained by cutting a bundle obtained by bundling a plurality of the gel-filled hollow tubular bodies (for example, hollow fibers) in a direction intersecting the longitudinal direction of the hollow tubular body. It is a gel microarray.
  • the present invention is a method for detecting a measurement object, comprising reacting a sample with the microarray and detecting a measurement target (for example, a nucleic acid such as DNA) in the sample.
  • a measurement target for example, a nucleic acid such as DNA
  • the length is 100 bases or less.
  • the present invention relates to a gel on which a biological substance is immobilized (a gel on which a biological substance is immobilized), wherein the gel contains a composition of ⁇ , ⁇ -dimethylacrylamide (2 to 7 mass./.). It is.
  • biological substance means a biological material that can be used as a capture probe, and includes, for example, deoxyribonucleic acid (DNA), ribonucleic acid (RNA), proteins, lipids, and the like. These bio-related substances can be obtained from commercial products or living cells.
  • DNA deoxyribonucleic acid
  • RNA ribonucleic acid
  • proteins proteins
  • lipids and the like.
  • DNA linear or circular plasmid DNA or chromosomal DNA is used. Furthermore, a restriction enzyme or a chemically-cleaved DNA fragment, a DNA synthesized by an enzyme or the like in a test tube, or a chemically synthesized oligonucleotide can also be used.
  • the biological substance prepared by the above method or the like is fixed to a gel-like substance (hereinafter, gel).
  • fixing means retaining a biological substance in the gel.
  • composition of the gel contains ⁇ , ⁇ -dimethylacrylamide in an amount of 2 to 7% by mass based on the mass of the gel, and the following composition is preferable.
  • the lower limit of ⁇ , ⁇ -dimethylacrylamide is preferably from 2.5 to 5.0% by mass.
  • the crosslinking agent is preferably a polyfunctional monomer having at least two ethylenically unsaturated bonds, in an amount of 0.1 to 1.5 mass relative to the gel mass. / 0 is preferable, and 0.3 to 0.7% by mass is more preferable.
  • the crosslinking agent is not particularly limited as long as it is the above-mentioned polyfunctional monomer, and examples thereof include methylenebisacrylamide, divinylbenzene, and polyethylene glycol di (meth) acrylate.
  • Gels can be prepared by mixing N, N-dimethylacrylamide and a crosslinking agent in an aqueous medium and copolymerizing them, or by polymerizing N, N-dimethylacrylamide into a prepolymer and then crosslinking. Examples thereof include a method of mixing and copolymerizing agents.
  • a method of immobilizing a bio-related substance on the above-mentioned gel a method of adding a bio-related substance having a vinyl group introduced into a terminal at the time of a polymerization reaction and copolymerizing with a gel component (see W002 / 62817),
  • An example is a method in which a hydrazine-treated gel is prepared and reacted with a bio-related substance having an amino group (see Japanese Patent Application Laid-Open No. 6-507486).
  • Water permeability of biological substances immobilized gel was prepared in the present invention, 1. 0 X 10_ 5 m 3 'ra' / m or more 2 / hr / MPa are preferred.
  • the water permeability of the gel is calculated from the amount of water passing through the gel.
  • the permeability test is defined as a value measured by the following method.
  • the shape retention of the gel is preferably 0.4 or more. More preferably, it is 0.6 or more.
  • the gel form retention is defined as a value measured by the following method.
  • the gel is prepared in a cylindrical container 13 inches in diameter and 4 cm in length. Remove the gel from the container and leave it in a closed container at 25 ° C for 24 hours to measure the height of the gel. Then, the form retention is calculated by the following equation.
  • Morphological retention gel height after standing for 24 hours No. 13111111 (initial gel diameter)
  • the bio-related substance-immobilized gel prepared by the above method is used as a gel for retaining the capture probe, as a tool for gene analysis. Used as
  • the use of the tubular body as an analysis tool for genes and the like was corrected. I) it can.
  • the introduction of the gel into the hollow portion is performed in the same manner as in the case of preparing a capillary ram used for capillary gel electrophoresis.
  • the gel of the present invention can also be used as a constituent member of a microarray.
  • a gel on which the above-mentioned capture probe is immobilized on a planar substrate hereinafter, immobilized gel
  • a microarray in which the immobilized gel is disposed in a plurality of sections on the planar substrate is manufactured.
  • a flat base having a plurality of grooves or through holes can also be used.
  • a monomer solution containing a bio-related substance before or immediately after the polymerization is added to the compartment formed by the groove or through-hole, and the polymerization reaction is carried out in the compartment, and cross-linking is performed.
  • a microarray in which a related substance-immobilized gel is arranged can be prepared (see JP-A-2000-60554).
  • the type of biological substance held in each compartment may be different for each compartment.
  • a plurality of immobilized gels of the same type may be grouped and arranged on a microarray.
  • the coordinates of the compartment can be determined.
  • the surface area of each compartment is usually, 10- 6 m 2 or less.
  • the lower limit is not particularly limited as long as the detection of a biological substance is possible.
  • examples of the hollow tubular body include a glass tube, a stainless steel tube, and a hollow fiber. Considering workability and ease of handling, it is preferred to use hollow fibers.
  • the fibers that can be used in the present invention include synthetic fibers, semi-synthetic fibers, regenerated fibers, chemical fibers such as inorganic fibers, and natural fibers (Japanese Patent Application Laid-Open No. 2000-270878).
  • synthetic fibers include various polyamide-based fibers such as nylon 6, nylon 66, and aromatic polyamide, and polyester-based fibers such as polyethylene terephthalate, polybutylene terephthalate, polylactic acid, and polyglycolic acid.
  • various fibers various acrylic fibers such as polyacrylonitrile, various polyolefin fibers such as polyethylene / polypropylene, various fibers of polyvinyl alcohol, various fibers of polyvinylidene chloride, polyvinyl chloride fibers, and polyurethane
  • resin fibers phenolic fibers, polyvinyl fluoride
  • Fluorinated fiber made of fluoroethylene etc., polystyrene
  • Fiber using (meth) acrylic resin such as polymethyl methacrylate Fiber using (meth) acrylic resin such as polymethyl methacrylate.
  • semi-synthetic fibers include various cellulose-based fiber derived from diacetate, triacetate, chitin, chitosan, and the like, and various protein-based fibers called promix.
  • Typical examples of the regenerated fiber include various cellulosic regenerated fibers (rayon, cuvula, polynosic, etc.) obtained by a viscose method, a copper-ammonia method, or an organic solvent method.
  • Representative examples of the inorganic fibers include glass fibers and carbon fibers.
  • Representative examples of natural fibers include vegetable fibers such as cotton, flax, ramie, jute, animal fibers such as wool and silk, and mineral fibers such as asbestos.
  • Hollow fibers other than natural fibers can be produced by a known method using a special nozzle.
  • the melt spinning method is preferred, and a horseshoe type nozzle, a C type nozzle, a double tube nozzle, or the like can be used as a nozzle.
  • Solvent spinning is preferably used for spinning synthetic polymers that cannot be melt-spun, semi-synthetic fibers or polymers used for regenerated fibers. Also in this case, a hollow fiber having a continuous hollow portion is obtained by spinning while filling the hollow portion with an appropriate liquid as a core material using a double tube nozzle similarly to the melt spinning.
  • the hollow tubular body prepared as described above can be used as a basic unit for holding the biomaterial-immobilized gel of the present invention.
  • a hollow tubular body for example, a plurality of the hollow tubular bodies described above are bundled, and the hollow portion of each hollow tubular body of the bundle is filled with a bio-related substance-immobilized gel, and the hollow body is aligned with the longitudinal direction of the tubular body.
  • a microarray bio-related substance-immobilized gel microarray
  • the gel may be packed after the individual hollow tubular bodies are filled with the gel.
  • the array is produced, for example, as follows. That is, two perforated plates that regularly form holes are prepared, and the hollow tubular body is passed through both holes so that the positions of the holes on one perforated plate correspond to the positions of the holes on the other perforated plate. . Next, the interval between the perforated plates is adjusted. However, the order of the operation of passing the hollow tubular body through the hole and the operation of adjusting the interval between the perforated plates may be reversed.
  • the cross-sectional shape of the array is not particularly limited.
  • the cross-section may be formed in a square or a rectangle by regularly arranging hollow tubes, and the hollow tubes are arranged concentrically.
  • the cross section may be formed in a circular shape.
  • a slice is obtained by cutting the above-described array in a direction intersecting with the longitudinal direction (axial direction of the hollow tubular body), preferably in a direction perpendicular to the longitudinal direction.
  • the cutting method include a method of cutting a slice from the array using a microtome.
  • the thickness of the flake can be arbitrarily adjusted, but is usually 1 to 5,, ⁇ ⁇ , preferably 10 to 2, ⁇ ⁇ ⁇ .
  • the obtained slice is used as a microarray holding a gel on which a biological substance is immobilized.
  • the biological substance immobilized on the gel in the microarray functions as a capture probe for nucleic acids or proteins that hybridize or bind to the biological substance (these are referred to as measurement targets). Therefore, the microarray of the present invention can be used as a kit for detecting a measurement target (nucleic acid, protein, etc.).
  • a sample containing a biologically relevant substance (eg, nucleic acid such as DNA) to be detected is prepared, added to the microarray, and reacted with the biologically relevant substance immobilized on the gel of the microarray.
  • a biologically relevant substance eg, nucleic acid such as DNA
  • the DNA to be measured is fluorescently labeled and hybridized with DNA in the microarray. After that, unreacted DNA is removed by washing, and the fluorescence intensity is detected. The fluorescence intensity can be measured using any DNA detector).
  • the “biomaterial-immobilized gel in which the biomaterial is immobilized on a gel containing 2 to 7% by mass of N, N-dimethylacrylamide” according to the present invention has good reactivity, The fluorescence intensity becomes uniform. As a result, highly sensitive detection results can be obtained.
  • FIG. 1 is a photograph showing the result of detecting DNA using the microarray of the present invention.
  • FIG. 2 is a photograph showing the result of detecting DNA using the microarray of the present invention.
  • an extruder is used from a spinning nozzle with an annular discharge port. To perform melt extrusion. As a result, a hollow fiber having an outer diameter of 0.3 mm, an inner diameter of 0.2 mm, and a length of 600 mm was obtained.
  • MMA methyl methacrylate
  • MA methyl acrylate
  • Oligonucleotides were synthesized using an automatic synthesizer DNA / RNA synthesizer (model 394, manufactured by PE Biosystems). In the final step of the synthesis, an amino group [NH 2 (CH 2 ) 6- ] was introduced at the 5 ′ terminal to synthesize oligonucleotide A (SEQ ID NO: 1) shown below. Similarly, oligonucleotide B (SEQ ID NO: 2) was synthesized (however, no amino group was introduced at the 5 'end). 5 'Introduction of Amino group at the end was used Aminorinku Iotaganma Micromax (manufactured by Applied Biosystems).
  • oligonucleotides were used after deprotection and purification by a general method.
  • Saccharomyces cerevisiae Saccharomyces cerevisiae (Saccharomyces cerevisiae) JCM7255 3 ⁇ 4r YPD medium (glucose 20 g / yeast extract 10 g / polypeptone 20 g / L pH 6.0) was cultured in 100 ml for 30 days, and then collected. Chromosomal DNA was prepared from the collected cells by a standard method and used for PCR type II.
  • PCR polymerase chain reaction
  • Capture probe A SEQ ID NO: 3
  • Polymerization solutions 1 and 2 having the compositions shown in Table 1 were prepared.
  • the monomer solution A and the polymerization initiator solution were prepared as follows.
  • 2, 2'-Azobis (2-inidazoline-2-yl) propane 1 g was dissolved in pure water to make a total volume of 10 ml.
  • the hollow portion of the three hollow fibers in the center row of the hollow fiber array obtained in (2) was filled with the polymer solution 1, and the hollow portions of the remaining hollow fibers were filled with the polymer solution 2.
  • the polymerization liquid 1 and the polymerization liquid 2 were charged.
  • the polymerization reaction was carried out by transferring the mixture to a sealed glass container whose inside was saturated with water vapor and leaving it to stand at 55 for 1 hour.
  • hollow fiber array After polymerization, hollow fiber array The cutting was repeated in a direction perpendicular to the longitudinal direction of the hollow fiber using a rubber. As a result, a flake with a thickness of about 500 zm was obtained.
  • Hybridization A hybridization solution containing 200 fmol / ml oligonucleotide C (SEQ ID NO: 4) complementary to a part of the base sequence of capture probe A (positions 241 to 339 of SEQ ID NO: 3) was prepared. Oligonucleotide C was synthesized using an automatic DNA synthesizer in the same manner as described in (3), and Cy5 was introduced at the 5 'end. After the completion of the synthesis, those deprotected and purified by a general method were used.
  • the slice obtained in (6) and 1 ml of the above hybridization solution were poured into a high prepack, and the upper end of the pack was heat-sealed.
  • the hybridization was performed at 65 for 20 hours.
  • the slices were taken out of the high prepack and washed sequentially under the conditions shown in Table 2.
  • the volume of the washing solution was 10 ml.
  • the washed slice was placed on a non-fluorescent slide glass, a few drops of sterilized water were dropped on the slice, and the glass was covered with a cover glass.
  • the slide glass was set on a DNA chip detector (GeneTac V: manufactured by Genomic Solutions) and detected using a laser for Cy5. The image was set to the size of one pixel ⁇ .
  • Fig. 1 shows the calculated fluorescence intensity and the image around the hollow fiber after washing, assuming the sum of the fluorescence intensity of 80 pixels around the center of the compartment as the compartment intensity. The center of the plot was determined arbitrarily. As a result, the distribution of the fluorescence intensity in the hybridized sections was uniform. Comparative Example 1>
  • Example 2 The same operation as in Example 1 was performed, except that the monomer solution ⁇ was changed to the monomer solution B.
  • Fig. 1 shows the fluorescence intensity and the image around the hollow fiber after washing.
  • Example 1 The same operation as in Example 1 was performed, except that the monomer liquid A was changed to the monomer liquid C.
  • FIG. 1 shows the fluorescence intensity and the image around the hollow fiber after washing. The fluorescence intensity was lower than in Example 1, and the fluorescence intensity in the hollow part was higher in the peripheral part and lower in the central part.
  • a slice was prepared in the same manner as in Example 1, except that the monomer solution A was changed to the monomer solution D and the capture probe A was changed to the capture probe B (SEQ ID NO: 5).
  • the capture probe B had a structure in which an amino group was introduced at the 5 ′ end and then reacted with glycidyl methacrylate to have a methacrylate group at the end.
  • Hybridization and washing were performed as follows.
  • Oligonucleotide E was synthesized using an automatic DNA synthesizer, and Cy5 was introduced at the 5 'end. After completion of the synthesis, those which were deprotected and purified by a general method were used.
  • the obtained flakes and 1 ml of the above hybridization solution were poured into a 0.02 SDS (sodium lauryl sulfate) high prepack, and the upper end of the pack was heat sealed.
  • the hybridization was performed at 37 for 16 hours.
  • the washed slice was placed on a non-fluorescent slide glass, a few drops of sterilized water were dropped on the slice, and a cover glass was covered.
  • the slide glass was set on a DNA chip detector (GeneTac IV: manufactured by Genomic Solutions), and the c- image detected using a laser for Cy5 was set to a size of 10 m per pixel. (4) Measurement of fluorescence intensity
  • the average fluorescence intensity for 200 pixels around the center of the plot was calculated as the plot intensity.
  • FIG. 2 shows the fluorescence intensity and the image around the hollow fiber after washing.
  • the center of the plot was arbitrarily determined.
  • the distribution of the fluorescence intensity in the hybridized compartment was uniform.
  • Example 3> The same operation as in Example 2 was performed except that the monomer liquid D was changed to the monomer liquid A.
  • FIG. 2 shows the fluorescence intensity of the compartment and the image around the hollow fiber after washing. The center of the plot was arbitrarily determined. As a result, the distribution of the fluorescence intensity in the hybridized compartment was uniform.
  • Example 2 The same operation as in Example 2 was performed except that the monomer liquid A was changed to the monomer liquid E.
  • FIG. 2 shows the fluorescence intensity and the image around the hollow fiber after washing. The fluorescence intensity was lower than in Example 2, and the fluorescence intensity in the hollow part was higher in the peripheral part and lower in the central part.
  • SEQ ID NO: 3 Synthetic DNA
  • SEQ ID NO: 5 synthetic DNA
  • SEQ ID NO: 6 synthetic DNA

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Abstract

La présente invention concerne un gel auquel est fixée une substance biologique, ladite substance biologique étant fixée à un gel contenant de 2 à 7 % en masse de N,N-diméthylacrylamide.
PCT/JP2003/004274 2002-04-03 2003-04-03 Gel auquel est fixee une substance biologique et microreseau comprenant ledit gel WO2003083475A1 (fr)

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US10/508,946 US20060084060A1 (en) 2002-04-03 2003-04-03 Gel having biosubstance fixed thereto and microarray utilizing the gel
JP2003580857A JP3654894B2 (ja) 2002-04-03 2003-04-03 生体関連物質固定化ゲル及び該ゲルを利用したマイクロアレイ
CA2481323A CA2481323C (fr) 2002-04-03 2003-04-03 Gel contenant une substance biologique immobilisee et microreseau utilisant ledit gel
AU2003236359A AU2003236359A1 (en) 2002-04-03 2003-04-03 Gel having biosubstance fixed thereto and microarray utilizing the gel
EP03745212A EP1491888B1 (fr) 2002-04-03 2003-04-03 Microreseau comprenant gel auquel est fixee une substance biologique ainsi que sa fabrication et son utilisation
IL16436603A IL164366A0 (en) 2002-04-03 2003-04-03 Biological substance-immobilized gel and microarray using the same
DE60323615T DE60323615D1 (de) 2002-04-03 2003-04-03 Mikroarray enthaltend gel mit fixierter biosubstanz sowie seine herstellung und verwendung
KR1020047015783A KR100989196B1 (ko) 2002-04-03 2003-04-03 생체관련 물질 고정화 겔 및 이 겔을 이용한 마이크로어레이
US12/014,251 US20090023601A1 (en) 2002-04-03 2008-01-15 Gel having biosubstance fixed thereto and microarray utilizing the gel
US13/098,613 US8802369B2 (en) 2002-04-03 2011-05-02 Gel having biosubstance fixed thereto and microarray utilizing the gel

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JP2009156767A (ja) * 2007-12-27 2009-07-16 National Institute Of Advanced Industrial & Technology タンパク質アレイ基板
JP2012223111A (ja) * 2011-04-18 2012-11-15 Mitsubishi Rayon Co Ltd 生体関連物質の検出方法
WO2014041940A1 (fr) 2012-09-14 2014-03-20 富士フイルム株式会社 Composition vulcanisable et procédé de formation d'image

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EP1595948A3 (fr) * 1999-03-05 2006-03-01 Mitsubishi Rayon Co., Ltd. Supports comportant une substance biologique
CA2628107C (fr) * 2005-11-01 2017-05-23 Northwestern University Systemes de polymeres matriciels et de polymeres dynamiques, et compositions pour la separation de microcanal
CA3147744C (fr) 2005-11-18 2024-04-23 Zoll Respicardia, Inc. Systeme et procede pour moduler le nerf phrenique et prevenir l'apnee du sommeil
US8909341B2 (en) 2007-01-22 2014-12-09 Respicardia, Inc. Device and method for the treatment of breathing disorders and cardiac disorders
US9987488B1 (en) 2007-06-27 2018-06-05 Respicardia, Inc. Detecting and treating disordered breathing
US8233987B2 (en) * 2009-09-10 2012-07-31 Respicardia, Inc. Respiratory rectification
CN103354838A (zh) * 2011-02-10 2013-10-16 三菱丽阳株式会社 核酸的检测方法
US20140274061A1 (en) * 2011-11-04 2014-09-18 Nokia Solutions And Networks Oy Anr establishment resolution
WO2013080941A1 (fr) * 2011-12-01 2013-06-06 三菱レイヨン株式会社 Base destinée à être utilisée dans l'amplification d'un acide nucléique, et procédé d'amplification d'acide nucléique

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CN100334229C (zh) * 2005-06-17 2007-08-29 东南大学 基于凝胶固定核酸的dna微阵列芯片制备方法
JP2009156767A (ja) * 2007-12-27 2009-07-16 National Institute Of Advanced Industrial & Technology タンパク質アレイ基板
JP2012223111A (ja) * 2011-04-18 2012-11-15 Mitsubishi Rayon Co Ltd 生体関連物質の検出方法
WO2014041940A1 (fr) 2012-09-14 2014-03-20 富士フイルム株式会社 Composition vulcanisable et procédé de formation d'image

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CA2481323A1 (fr) 2003-10-09
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EP1491888A1 (fr) 2004-12-29
JP3654894B2 (ja) 2005-06-02
KR20050008671A (ko) 2005-01-21
EP1491888A4 (fr) 2006-05-03
DE60323615D1 (de) 2008-10-30
KR100989196B1 (ko) 2010-10-20
CA2481323C (fr) 2013-12-24
ATE408451T1 (de) 2008-10-15
JPWO2003083475A1 (ja) 2005-08-04
CN1650164A (zh) 2005-08-03
US8802369B2 (en) 2014-08-12
US20090023601A1 (en) 2009-01-22
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