WO2002010349A1 - Cultured epidermal cell sheet, laminated cultured skin sheet and process for producing the same - Google Patents

Cultured epidermal cell sheet, laminated cultured skin sheet and process for producing the same Download PDF

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Publication number
WO2002010349A1
WO2002010349A1 PCT/JP2001/005723 JP0105723W WO0210349A1 WO 2002010349 A1 WO2002010349 A1 WO 2002010349A1 JP 0105723 W JP0105723 W JP 0105723W WO 0210349 A1 WO0210349 A1 WO 0210349A1
Authority
WO
WIPO (PCT)
Prior art keywords
cultured
sheet
laminated
temperature
epidermal cell
Prior art date
Application number
PCT/JP2001/005723
Other languages
English (en)
French (fr)
Inventor
Teruo Okano
Masayuki Yamato
Mika Utsumi
Ai Kushida
Chie Konno
Akihiko Kikuchi
Original Assignee
Cellseed Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cellseed Inc. filed Critical Cellseed Inc.
Priority to EP01945763A priority Critical patent/EP1312669B1/en
Priority to AT01945763T priority patent/ATE524541T1/de
Priority to US10/333,468 priority patent/US8889417B2/en
Priority to JP2002516068A priority patent/JP4716479B2/ja
Publication of WO2002010349A1 publication Critical patent/WO2002010349A1/ja

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
    • A61L27/362—Skin, e.g. dermal papillae
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3886—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells comprising two or more cell types
    • A61L27/3891—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells comprising two or more cell types as distinct cell layers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/60—Materials for use in artificial skin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • C12N5/0698—Skin equivalents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/09—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells
    • C12N2502/094—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells keratinocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1323—Adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2539/00—Supports and/or coatings for cell culture characterised by properties
    • C12N2539/10—Coating allowing for selective detachment of cells, e.g. thermoreactive coating

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Epidemiology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Dermatology (AREA)
  • Medicinal Chemistry (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Transplantation (AREA)
  • Animal Behavior & Ethology (AREA)
  • Botany (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Urology & Nephrology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Materials For Medical Uses (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
PCT/JP2001/005723 2000-07-21 2001-07-02 Cultured epidermal cell sheet, laminated cultured skin sheet and process for producing the same WO2002010349A1 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP01945763A EP1312669B1 (en) 2000-07-21 2001-07-02 Cultured epidermal cell sheet, laminated cultured skin sheet and process for producing the same
AT01945763T ATE524541T1 (de) 2000-07-21 2001-07-02 Kultivierte epidermale zellschicht, laminierte kultivierte hautschicht und verfahren zu deren herstellung
US10/333,468 US8889417B2 (en) 2000-07-21 2001-07-02 Epidermal cultured cell sheet, multi-layered cultured skin sheet and processes for producing those sheets
JP2002516068A JP4716479B2 (ja) 2000-07-21 2001-07-02 表皮培養細胞シート、重層化培養皮膚シート、及びそれらの製造法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2000221383 2000-07-21
JP2000-221383 2000-07-21

Publications (1)

Publication Number Publication Date
WO2002010349A1 true WO2002010349A1 (en) 2002-02-07

Family

ID=18715794

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2001/005723 WO2002010349A1 (en) 2000-07-21 2001-07-02 Cultured epidermal cell sheet, laminated cultured skin sheet and process for producing the same

Country Status (6)

Country Link
US (1) US8889417B2 (ja)
EP (3) EP1312669B1 (ja)
JP (1) JP4716479B2 (ja)
AT (1) ATE524541T1 (ja)
ES (1) ES2369253T3 (ja)
WO (1) WO2002010349A1 (ja)

Cited By (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004069295A1 (ja) * 2003-02-06 2004-08-19 Cellseed Inc. 角膜上皮形成用細胞シート、それらの製造方法及びそれらの利用方法
WO2004073761A1 (ja) * 2003-02-20 2004-09-02 Cellseed Inc. 再生角膜内皮細胞シート、製造方法及びその利用方法
WO2005047496A1 (ja) * 2003-11-14 2005-05-26 Keio University 細胞培養法、細胞の三次元培養法、三次元組織、人工臓器、及び組織移植方法
EP1598417A1 (en) * 2003-02-06 2005-11-23 Cellseed Inc. Anterior ocular segment related cell sheet, three-dimensional structures and process for producing the same
JP2007229249A (ja) * 2006-03-01 2007-09-13 Olympus Corp 細胞シートの供給方法
JP2007229251A (ja) * 2006-03-01 2007-09-13 Olympus Corp 細胞シートの搬送方法および取付装置
JPWO2005103233A1 (ja) * 2004-04-25 2008-03-13 株式会社セルシード 培養歯根膜細胞シート、製造方法及びその利用方法
JP2008231083A (ja) * 2007-03-19 2008-10-02 Cellseed Inc 移植用成熟誘導化樹状細胞、製造方法及びその利用方法
WO2010027081A1 (ja) * 2008-09-08 2010-03-11 学校法人東京理科大学 スフェロイド複合体およびスフェロイド含有ハイドロゲルならびにその製造方法
JP2010063375A (ja) * 2008-09-08 2010-03-25 Tokyo Univ Of Science スフェロイド複合体およびその製造方法、ならびに多層型スフェロイド複合体
JP2010065082A (ja) * 2008-09-08 2010-03-25 Tokyo Univ Of Science スフェロイド含有ハイドロゲルおよびその製造方法、ならびにスフェロイド含有ハイドロゲル積層体
JP2010516273A (ja) * 2007-01-24 2010-05-20 コーニンクレッカ フィリップス エレクトロニクス エヌ ヴィ 培養細胞を処理する方法
JP2010161952A (ja) * 2009-01-13 2010-07-29 Dainippon Printing Co Ltd 生体組織の作製方法
JP2010161954A (ja) * 2009-01-13 2010-07-29 Dainippon Printing Co Ltd 生体組織の作製方法
JP2010161953A (ja) * 2009-01-13 2010-07-29 Dainippon Printing Co Ltd 生体組織の作製方法
WO2010134619A1 (ja) * 2009-05-18 2010-11-25 国立大学法人東北大学 人工多能性幹細胞からの上皮系前駆細胞・幹細胞群及び角膜上皮細胞群の分化誘導方法
EP2574664A1 (en) 2011-09-30 2013-04-03 Centrum Materialów Polimerowych i Weglowych PAN Method for preparation a thermosensitive coating substrate, the substrate with a thermosensitive coating and its application
JP2013081791A (ja) * 2005-02-28 2013-05-09 Cellseed Inc 培養細胞シート、製造方法及びその利用した組織修復方法
JP2013521809A (ja) * 2010-03-22 2013-06-13 フラウンホーファー−ゲゼルシャフト ツル フェルデルング デル アンゲヴァンテン フォルシュング エー ファウ ミクロゲルを有する熱応答性基質、その調整方法、および生物細胞の培養方法
JP2013143963A (ja) * 2006-01-12 2013-07-25 Tokyo Women's Medical College 肝組織細胞機能の長期維持方法
JP2014023540A (ja) * 2013-11-06 2014-02-06 Dainippon Printing Co Ltd 生体組織の作製方法
US8859279B2 (en) 2006-06-20 2014-10-14 Hitachi High-Tech Science Corporation Cell detachment method
US9090869B2 (en) 2010-06-10 2015-07-28 Kyushu Institute Of Technology Temperature responsive sheet that displays reversible properties and cell sheet production method using same
JP2021507718A (ja) * 2017-12-29 2021-02-25 ジーンメディスン・カンパニー・リミテッド 遺伝子伝達用細胞シート

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WO2004069988A1 (ja) * 2003-02-06 2004-08-19 Cellseed Inc. 高密度細胞アレイ用基板、製造法、及びその利用方法
WO2006071794A2 (en) 2004-12-23 2006-07-06 Ethicon Incorporated Postpartum cells derived from umbilical cord tissue, and methods of making and using the same
WO2005001080A2 (en) * 2003-06-27 2005-01-06 Ethicon, Incorporated Postpartum-derived cells for use in treatment of disease of the heart and circulatory system
US7875272B2 (en) 2003-06-27 2011-01-25 Ethicon, Incorporated Treatment of stroke and other acute neuraldegenerative disorders using postpartum derived cells
US20060166361A1 (en) * 2004-12-21 2006-07-27 Agnieszka Seyda Postpartum cells derived from placental tissue, and methods of making, culturing, and using the same
US20060171930A1 (en) * 2004-12-21 2006-08-03 Agnieszka Seyda Postpartum cells derived from umbilical cord tissue, and methods of making, culturing, and using the same
EP1857126B1 (en) * 2005-02-28 2018-10-24 CellSeed Inc. Cultured cell sheet, production method thereof, and application method thereof
GB2442271B (en) * 2006-09-27 2011-06-15 Denis Eon Solomon An in vitro human skin assay protocol which directly uses the natural cellular interactions between un-manipulated autologous human epidermal and dermal cells
US8105380B2 (en) * 2006-10-23 2012-01-31 Stemedica Cell Technologies, Inc. Cellular scaffold
US8709081B2 (en) 2006-10-23 2014-04-29 Stemedica Cell Technologies, Inc. Cellular scaffold
KR100871652B1 (ko) 2007-07-30 2008-12-02 한양대학교 산학협력단 세포 배양용 3차원 마이크로 캐리어, 이의 제조방법 및이를 이용한 세포의 3차원 부유 배양법
EP2348099B1 (en) * 2008-10-14 2017-07-05 CellSeed Inc. Temperature-responsive cell culture substrate and method for producing the same
US20100166822A1 (en) * 2008-12-31 2010-07-01 Howmedica Osteonics Corp. Adhesive cartilage implant
US9546349B2 (en) * 2011-05-31 2017-01-17 Taipei Medical University Supports for cell culture and cell sheet detachment and methods for cell sheet detachment
TWI452138B (zh) * 2011-06-01 2014-09-11 Univ Taipei Medical 用於細胞培養及片狀細胞層脫著之支撐物及片狀細胞層脫著之方法
JP6781973B2 (ja) * 2016-01-29 2020-11-11 学校法人東京女子医科大学 腎臓病進行抑制細胞シート組成物、その製造方法、及び、それを用いた腎臓病進行抑制方法
CN106924817B (zh) * 2017-03-02 2019-12-17 浙江大学 一种超薄载体细胞片及其制备方法
CN112512601A (zh) * 2018-07-31 2021-03-16 罗基医疗保健公司 多层细胞膜片的制备方法及利用其制备的多层细胞膜片

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KR101508772B1 (ko) 2003-02-06 2015-04-14 가부시키가이샤 셀시드 전안부 관련 세포 시트, 3 차원 구조체, 및 그들의 제조법
EP2450065A1 (en) * 2003-02-06 2012-05-09 Cellseed Inc. Corneal epithelium forming cell sheets, processes for producing the same, and methods of using the same
EP1598417A1 (en) * 2003-02-06 2005-11-23 Cellseed Inc. Anterior ocular segment related cell sheet, three-dimensional structures and process for producing the same
EP1602383A1 (en) * 2003-02-06 2005-12-07 Cellseed Inc. Cell sheets for ectocornea formation, method of producing the same and method of using the same
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US8642338B2 (en) 2003-02-06 2014-02-04 Cellseed Inc. Anterior ocular segment related cell sheets, three-dimensional structures, and processes for producing the same
EP2230298A1 (en) * 2003-02-06 2010-09-22 Cellseed Inc. Anterior ocular segment related cell sheets, three-dimensional structures, and processes for producing the same
WO2004069295A1 (ja) * 2003-02-06 2004-08-19 Cellseed Inc. 角膜上皮形成用細胞シート、それらの製造方法及びそれらの利用方法
US10434219B2 (en) 2003-02-06 2019-10-08 Cellseed Inc. Method of treatment using corneal epithelium forming cell sheets
EP1602383A4 (en) * 2003-02-06 2009-07-22 Cellseed Inc CELLULAR SHEETS FOR ECTOCORNEE FORMATION, CORRESPONDING PRODUCTION PROCESS AND METHOD OF USING THE SAME
CN100436577C (zh) * 2003-02-06 2008-11-26 赛尔斯德株式会社 前眼部相关细胞片、三维结构体、及它们的制造方法
WO2004073761A1 (ja) * 2003-02-20 2004-09-02 Cellseed Inc. 再生角膜内皮細胞シート、製造方法及びその利用方法
US9981064B2 (en) 2003-02-20 2018-05-29 Cellseed Inc. Regenerated corneal endothelial cell sheets, processes for producing the same, and methods of using the same
WO2005047496A1 (ja) * 2003-11-14 2005-05-26 Keio University 細胞培養法、細胞の三次元培養法、三次元組織、人工臓器、及び組織移植方法
JPWO2005047496A1 (ja) * 2003-11-14 2007-05-31 学校法人慶應義塾 細胞培養法、細胞の三次元培養法、三次元組織、人工臓器、及び組織移植方法
US9114192B2 (en) 2004-04-25 2015-08-25 Cellseed Inc. Cultured periodontal ligament cell sheet, process for producing the same and method of use thereof
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ATE524541T1 (de) 2011-09-15

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