DK1264877T3 - Celledyrkningsbæremateriale, fremgangsmåde til samdyrkning af celler og samdyrket cellelag opnået derved - Google Patents

Celledyrkningsbæremateriale, fremgangsmåde til samdyrkning af celler og samdyrket cellelag opnået derved

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Publication number
DK1264877T3
DK1264877T3 DK01912451.0T DK01912451T DK1264877T3 DK 1264877 T3 DK1264877 T3 DK 1264877T3 DK 01912451 T DK01912451 T DK 01912451T DK 1264877 T3 DK1264877 T3 DK 1264877T3
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DK
Denmark
Prior art keywords
culture
cells
support material
layer obtained
cell
Prior art date
Application number
DK01912451.0T
Other languages
English (en)
Inventor
Akihiko Kikuchi
Teruo Okano
Masayuki Yamato
Original Assignee
Cellseed Inc
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Publication date
Application filed by Cellseed Inc filed Critical Cellseed Inc
Application granted granted Critical
Publication of DK1264877T3 publication Critical patent/DK1264877T3/da

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0618Cells of the nervous system
    • C12N5/0619Neurons
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/08Chemical, biochemical or biological means, e.g. plasma jet, co-culture
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068General culture methods using substrates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/067Hepatocytes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0688Cells from the lungs or the respiratory tract
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00Coculture with; Conditioned medium produced by
    • C12N2502/13Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1323Adult fibroblasts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00Coculture with; Conditioned medium produced by
    • C12N2502/28Vascular endothelial cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2535/00Supports or coatings for cell culture characterised by topography
    • C12N2535/10Patterned coating
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2539/00Supports and/or coatings for cell culture characterised by properties
    • C12N2539/10Coating allowing for selective detachment of cells, e.g. thermoreactive coating

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Neurology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Neurosurgery (AREA)
  • Molecular Biology (AREA)
  • Physiology (AREA)
  • Sustainable Development (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Pulmonology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
DK01912451.0T 2000-03-16 2001-03-16 Celledyrkningsbæremateriale, fremgangsmåde til samdyrkning af celler og samdyrket cellelag opnået derved DK1264877T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2000074269 2000-03-16
PCT/JP2001/002123 WO2001068799A1 (fr) 2000-03-16 2001-03-16 Materiau de support destine a la culture de cellules, procede de coculture de cellules et feuillet de cellules cocultivees ainsi obtenu

Publications (1)

Publication Number Publication Date
DK1264877T3 true DK1264877T3 (da) 2013-10-28

Family

ID=18592355

Family Applications (1)

Application Number Title Priority Date Filing Date
DK01912451.0T DK1264877T3 (da) 2000-03-16 2001-03-16 Celledyrkningsbæremateriale, fremgangsmåde til samdyrkning af celler og samdyrket cellelag opnået derved

Country Status (5)

Country Link
US (1) US7405078B2 (da)
EP (2) EP1264877B1 (da)
JP (1) JP4567936B2 (da)
DK (1) DK1264877T3 (da)
WO (1) WO2001068799A1 (da)

Families Citing this family (72)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3975266B2 (ja) * 2002-05-24 2007-09-12 独立行政法人産業技術総合研究所 細胞培養装置
US6943021B2 (en) * 2002-06-07 2005-09-13 Mattek Corporation Three dimensional vaginal tissue model containing immune cells
EP2230298A1 (en) * 2003-02-06 2010-09-22 Cellseed Inc. Anterior ocular segment related cell sheets, three-dimensional structures, and processes for producing the same
EP1602383B1 (en) 2003-02-06 2015-05-27 Cellseed Inc. Cell sheets for ectocornea formation, method of producing the same and method of using the same
US20060240400A1 (en) * 2003-02-06 2006-10-26 Masayuki Yamato High-density cell array board, process for producing the same and method of using the same
US9981064B2 (en) 2003-02-20 2018-05-29 Cellseed Inc. Regenerated corneal endothelial cell sheets, processes for producing the same, and methods of using the same
JP4756816B2 (ja) * 2003-03-10 2011-08-24 株式会社アドバンス 骨再生補綴物の製造方法
CA2527728A1 (en) * 2003-05-30 2004-12-16 Board Of Regents, The University Of Texas System Micropositioning cells for tissue engineering
US20080050812A1 (en) * 2003-05-30 2008-02-28 Cox James W Micropositioning Cells For Tissue Engineering
JP5148873B2 (ja) 2003-06-27 2013-02-20 エチコン、インコーポレイテッド 臍帯組織由来の分娩後細胞、及びその作成及び使用方法
WO2006071794A2 (en) 2004-12-23 2006-07-06 Ethicon Incorporated Postpartum cells derived from umbilical cord tissue, and methods of making and using the same
US7875272B2 (en) 2003-06-27 2011-01-25 Ethicon, Incorporated Treatment of stroke and other acute neuraldegenerative disorders using postpartum derived cells
JP2005034060A (ja) * 2003-07-15 2005-02-10 Sumitomo Bakelite Co Ltd 生化学研究用器具
JP2005052011A (ja) * 2003-08-04 2005-03-03 Institute Of Physical & Chemical Research マイクロパターン化細胞基質およびその製造方法
CA2539613A1 (en) * 2003-09-19 2005-03-31 Keio University Compositions for use in coating of substrate for cell sheet preparation, substrates for cell sheet preparation, and method for manufacturing cell sheets
WO2006019043A1 (ja) * 2004-08-17 2006-02-23 Kyushu Institute Of Technology 細胞培養用多孔性シート状物とそれを用いたバイオリアクター及び培養方法
US20060166361A1 (en) * 2004-12-21 2006-07-27 Agnieszka Seyda Postpartum cells derived from placental tissue, and methods of making, culturing, and using the same
WO2006093153A1 (ja) 2005-02-28 2006-09-08 Cellseed Inc. 培養細胞シート、製造方法及びその利用した組織修復方法
EP1857126B1 (en) * 2005-02-28 2018-10-24 CellSeed Inc. Cultured cell sheet, production method thereof, and application method thereof
JP2008545410A (ja) * 2005-05-24 2008-12-18 ザ レジェンツ オブ ザ ユニヴァースティ オブ カリフォルニア 微小規模のマイクロパターン化インビトロ操作・作製組織
JP2008289362A (ja) * 2005-09-01 2008-12-04 Univ Of Tokyo マイクロパターニング培養基板、マイクロパターニング培養構築物及びこれらの作成方法
EP1951741A4 (en) * 2005-10-27 2011-06-01 Life Technologies Corp SURFACE MODIFICATION IN A HANDLING ROOM
KR20190104428A (ko) 2005-12-29 2019-09-09 안트로제네시스 코포레이션 태반 줄기 세포 집단
EP1873205A1 (en) * 2006-06-12 2008-01-02 Corning Incorporated Thermo-responsive blends and uses thereof
US20080108138A1 (en) * 2006-06-13 2008-05-08 Vermette Patrick Bioactive compositions and their use in cell patterning
WO2008005520A2 (en) * 2006-07-06 2008-01-10 Organogenesis Inc. Temperature-responsive microcarrier
JP5080848B2 (ja) * 2007-04-20 2012-11-21 大日本印刷株式会社 細胞培養支持体とその製造方法
US8557583B2 (en) 2007-03-15 2013-10-15 Dai Nippon Printing Co., Ltd. Cell culture support and manufacture thereof
JP2008228585A (ja) * 2007-03-16 2008-10-02 Canon Inc 細胞培養容器および細胞培養装置
JP5393662B2 (ja) * 2007-06-22 2014-01-22 イノベイティブ サーフェイス テクノロジーズ, インコーポレイテッド 潜在反応基を含有するナノ繊維
EP2171135A2 (en) 2007-06-22 2010-04-07 Innovative Surface Technologies, Inc. Stimuli responsive nanofibers
WO2009045360A2 (en) 2007-09-28 2009-04-09 Celgene Cellular Therapeutics Tumor suppression using human placental perfusate and human placenta-derived intermediate natural killer cells
EP2231858B1 (en) * 2007-12-10 2015-11-04 Koninklijke Philips N.V. Patterned cell sheets and a method for production of the same
JPWO2010010837A1 (ja) * 2008-07-25 2012-01-05 コニカミノルタホールディングス株式会社 細胞培養支持体および細胞培養方法
US8828376B2 (en) 2008-08-20 2014-09-09 Anthrogenesis Corporation Treatment of stroke using isolated placental cells
KR20240052847A (ko) 2008-08-20 2024-04-23 셀룰래리티 인코포레이티드 개선된 세포 조성물 및 그의 제조 방법
US8728805B2 (en) 2008-08-22 2014-05-20 Anthrogenesis Corporation Methods and compositions for treatment of bone defects with placental cell populations
JP5396803B2 (ja) * 2008-10-06 2014-01-22 コニカミノルタ株式会社 細胞培養基材及び細胞培養方法
US9598668B2 (en) * 2008-10-14 2017-03-21 Cellseed Inc. Temperature-responsive cell culture substrate and method for producing the same
US20100166822A1 (en) * 2008-12-31 2010-07-01 Howmedica Osteonics Corp. Adhesive cartilage implant
US9469839B2 (en) * 2009-06-29 2016-10-18 General Electric Company Cell culture support and associated method for cell growth and release
WO2011016423A1 (ja) * 2009-08-02 2011-02-10 学校法人 東京女子医科大学 膵島細胞シート、製造方法及びその利用方法
JPWO2011024963A1 (ja) * 2009-08-27 2013-01-31 学校法人東京女子医科大学 直鎖型温度応答性高分子が固定化された温度応答性細胞培養基材、及びその製造方法
EP3284818B1 (en) 2010-01-26 2022-03-09 Celularity Inc. Treatment of bone-related cancers using placental stem cells
TW201902496A (zh) 2010-04-07 2019-01-16 美商安瑟吉納西斯公司 利用胎盤幹細胞之血管新生
AU2011237743A1 (en) 2010-04-08 2012-11-01 Anthrogenesis Corporation Treatment of sarcoidosis using placental stem cells
KR20190108599A (ko) 2010-07-13 2019-09-24 안트로제네시스 코포레이션 천연 킬러 세포의 생성 방법
US9453196B2 (en) * 2010-12-16 2016-09-27 General Electric Company Cell carrier, methods of making and use
US9453197B2 (en) * 2010-12-16 2016-09-27 General Electric Company Methods of making cell carrier
US20120156772A1 (en) * 2010-12-16 2012-06-21 General Electric Company Cell carrier and methods of making
US9534206B2 (en) 2010-12-16 2017-01-03 General Electric Company Cell carrier, associated methods for making cell carrier and culturing cells using the same
US9518249B2 (en) 2010-12-16 2016-12-13 General Electric Company Cell carrier, associated methods for making cell carrier and culturing cells using the same
US9926523B2 (en) 2010-12-16 2018-03-27 General Electric Company Cell carriers and methods for culturing cells
US8969315B2 (en) 2010-12-31 2015-03-03 Anthrogenesis Corporation Enhancement of placental stem cell potency using modulatory RNA molecules
CN113559126A (zh) 2011-06-01 2021-10-29 人类起源公司 利用胎盘干细胞治疗疼痛
PT2574664E (pt) 2011-09-30 2014-10-03 Ct Materialów Polimerowych I Weglowych Pan Método para a preparação de um substrato com revestimento termossensível, o substrato com um revestimento termossensível e sua aplicação
EP2781594A4 (en) * 2011-11-20 2015-04-08 Univ Tokyo Womens Medical CELL CULTURE SUBSTRATE AND METHOD FOR MANUFACTURING THE SAME
DE102012101240A1 (de) * 2012-02-16 2013-08-22 Technische Universität Ilmenau Verfahren zur bestimmung der ansiedelbarkeit von biologischen zellen auf strukturen aus einem polymer sowie verfahren zur herstellung solcher strukturen
EP2899262B1 (en) * 2012-09-19 2017-08-09 Japan Science and Technology Agency Formation and use of neuronal network, and neuron seeding device
CN115137753A (zh) 2013-02-05 2022-10-04 细胞结构公司 来自胎盘的自然杀伤细胞
WO2014186430A1 (en) 2013-05-16 2014-11-20 Trustees Of Boston University Multi-layered cell constructs and methods of use and production using enzymatically degradable natural polymers
JP6349638B2 (ja) * 2013-07-16 2018-07-04 大日本印刷株式会社 細胞培養基材の製造方法
JP2016049083A (ja) * 2014-09-02 2016-04-11 大日本印刷株式会社 パターンを有する温度応答性細胞培養基材の製造方法
WO2016052472A1 (ja) 2014-09-29 2016-04-07 公立大学法人横浜市立大学 三次元細胞集合体の作製方法
KR20240001270A (ko) * 2015-06-26 2024-01-03 고쿠리츠켄큐카이하츠호진 고쿠리츠쥰칸키뵤 겐큐센터 세포배양기
JP6780816B2 (ja) * 2016-03-16 2020-11-04 国立研究開発法人国立循環器病研究センター 上皮系細胞の培養方法、細胞構造体の製造方法、及び上皮系細胞用細胞培養器
EP3409763A4 (en) * 2016-01-29 2019-10-30 National Cerebral and Cardiovascular Center CELLULAR AMAS, CELL STRUCTURE AND THREE DIMENSIONAL TISSUE BODY
WO2017131241A1 (ja) 2016-01-29 2017-08-03 国立研究開発法人国立循環器病研究センター 細胞塊、細胞構造体及び立体組織体
DE102018123553A1 (de) * 2018-09-25 2020-03-26 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Bioreaktor und Verfahren zur Kultivierung biologischer Zellen an Substratfilamenten
DE102019106011A1 (de) * 2019-03-08 2020-09-10 Universität Siegen Verfahren zur zelltrennung unter verwendung eines stimulus-responsiven polymers
CN109762780A (zh) * 2019-03-29 2019-05-17 北京昱龙摩尔国际生物医学研究院 一种人肺泡上皮细胞分离与培养方法
EP3984596A4 (en) 2019-06-14 2023-06-21 Kaneka Corporation POPULATION OF CELLS COMPRISING MESENCHYMATOUS CELLS, PHARMACEUTICAL COMPOSITION COMPRISING THEM AND METHOD FOR PRODUCTION THEREOF

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0387975A1 (en) * 1989-03-16 1990-09-19 W.R. Grace & Co.-Conn. Cell culture substrate cell sheet, cell cluster and preparations thereof
JP3139004B2 (ja) 1990-04-20 2001-02-26 東レ株式会社 細胞培養基材およびそれを用いる細胞培養方法
JPH05168470A (ja) * 1990-08-08 1993-07-02 W R Grace & Co 複数の細胞種からなる細胞塊状体とシートおよびその製造法
JPH0494679A (ja) 1990-08-08 1992-03-26 Kao Corp 細胞培養支持体材料
EP0470681A3 (en) * 1990-08-08 1992-04-22 W.R. Grace & Co.-Conn. Cell clusters or sheets comprising a plurality of cell species and preparations thereof
JPH04237492A (ja) 1991-01-21 1992-08-25 Terumo Corp 細胞培養用成形物
EP0499932A1 (en) * 1991-02-16 1992-08-26 W.R. Grace & Co.-Conn. Cancer cell cluster comprising cancer cells and normal cells and preparation thereof
EP0552380A4 (en) * 1991-08-08 1995-01-25 Kao Corp Cell culture support, production thereof, and production of cell cluster using same
JPH05244938A (ja) * 1992-01-22 1993-09-24 Kao Corp 細胞及びその培養法
JPH05260957A (ja) * 1992-01-22 1993-10-12 Kao Corp 肝細胞スフェロイド及びその調製法
JP3126269B2 (ja) 1993-09-07 2001-01-22 株式会社バイオマテリアル研究所 培養基質
JPH0923876A (ja) * 1995-07-11 1997-01-28 Kao Corp 細胞培養支持体の製造法

Also Published As

Publication number Publication date
JP4567936B2 (ja) 2010-10-27
EP1264877A4 (en) 2007-06-27
WO2001068799A1 (fr) 2001-09-20
EP1264877A1 (en) 2002-12-11
US20030036196A1 (en) 2003-02-20
EP1264877B1 (en) 2013-10-02
US7405078B2 (en) 2008-07-29
EP2298858A1 (en) 2011-03-23

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