WO2002003078A1 - Procede et dispositif de distribution de liquide - Google Patents
Procede et dispositif de distribution de liquide Download PDFInfo
- Publication number
- WO2002003078A1 WO2002003078A1 PCT/JP2000/004390 JP0004390W WO0203078A1 WO 2002003078 A1 WO2002003078 A1 WO 2002003078A1 JP 0004390 W JP0004390 W JP 0004390W WO 0203078 A1 WO0203078 A1 WO 0203078A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- liquid
- dispensing
- dispensed
- tip
- sucked
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
- G01N35/1016—Control of the volume dispensed or introduced
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/14—Process control and prevention of errors
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/10—Means to control humidity and/or other gases
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/0241—Drop counters; Drop formers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
- G01N2035/1025—Fluid level sensing
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/2575—Volumetric liquid transfer
Definitions
- the present invention relates to a liquid dispensing method and apparatus, particularly to a liquid dispensing method and apparatus suitable for use in an automatic analyzer.
- An automatic analyzer for dispensing a reagent and a sample into a reaction container to react with each other and measuring the reacted reaction solution is provided with a liquid dispensing device for dispensing the sample and the reagent into the reaction container.
- a dispensing probe made of stainless steel is dispensed by directly contacting a sample, which is a liquid to be dispensed, with a reagent, and dispensing the sample. After dispensing the probe to be dispensed, wash the part in contact with the liquid to be dispensed, and use it repeatedly.Attach the dispensing tip to the tip of the dispensing probe, perform dispensing, and end the dispensing. There is a method of replacing the dispensing tip every time. This method using a dispensing tip can avoid carryover between samples, between reagents, or between a sample and a reagent. In the measurement of immunity items with a wide measurement concentration range, carry-over between samples is a particular problem, and the dispensing method using a dispensing tip is often used for dispensing samples.
- the dispensed amount may fluctuate due to changes in environmental temperature and humidity. This is because the saturated vapor pressure of the liquid to be dispensed changes depending on the temperature and humidity of the environment, and consequently the vapor pressure of the gas phase inside the dispensing tip changes. This is because the suction amount of the liquid to be dispensed fluctuates even if is kept constant. ⁇
- the pipetting tube has a larger contact area between the liquid to be aspirated and the air in the pipetting tip or pipetting pipe than the stainless steel pipetting probe, so The vapor pressure of the air inside the dispensing tip changes greatly due to evaporation of the liquid to be dispensed during operation, and is susceptible to changes in environmental temperature and humidity.
- a sensor detects the height of the liquid sucked into the dispensing nozzle.
- a method of sucking a predetermined amount of liquid is disclosed in Japanese Patent Application Laid-Open No. 9-28811.
- An object of the present invention is to provide a liquid dispensing method and apparatus suitable for reducing the influence of a change in environmental temperature or humidity on the liquid dispensed amount.
- the non-reactive liquid is aspirated by the dispensing probe, after which the dispensing probe draws air first at a first velocity, then at a second velocity higher than the first velocity, Thereafter, the liquid to be dispensed is sucked into the dispensing probe. The sucked liquid to be dispensed is then discharged from the dispensing probe.
- the method of aspirating a predetermined amount of water into the dispensing tip, discharging the water out of the dispensing tip, and then aspirating the liquid to be dispensed requires accurate liquid dispensing volume. And the reproducibility was not sufficient. This is thought to be due to the low and unstable relative humidity of the air inside the dispensing tip.
- FIG. 1 is a schematic view of a liquid dispensing apparatus showing one embodiment according to the present invention.
- FIG. 2 is an operation flow chart of the dispensing probe before the liquid to be dispensed is sucked into the dispensing tip.
- FIG. 3 is a schematic diagram of one embodiment of an automatic analyzer to which the liquid dispensing device according to the present invention is applied.
- FIG. 4 is a flowchart of the entire dispensing operation in the embodiment of FIG.
- FIG. 5 is a flowchart of sample dispensing.
- FIG. 1 shows an embodiment of a liquid dispensing apparatus according to the present invention.
- the conductive dispensing probe 1 has a conductive dispensing tube 2 at its tip. This dispensing probe is detachable so that it can be disposable.
- Dispensing probe 1 is connected to syringe 4 via tube 3.
- the syringe 4 is driven by the syringe drive mechanism 11, and the syringe drive mechanism 11 is controlled by the control unit 7.
- the pump 32 sends the water from the water tank 13 so that the inside of the syringe 4, the tube 3, and the dispensing probe 1 is filled with water before the liquid dispensing operation of the liquid dispensing device. In that case, the valve 31 is opened.
- the water transmits the operation of the syringe 4 to the liquid to be dispensed in order to suck the liquid to be dispensed into the dispensing tip 2 and to discharge the sucked liquid to be dispensed from the dispensing tip 2.
- the transmission medium is indicated by reference numeral 30 in the figure.
- the dispensing probe 1 can be moved up and down by a dispensing probe vertical movement mechanism 5 driven by a motor 6, and the motor 6 is controlled by a control unit 7.
- the dispensing probe 1 can be moved horizontally between the dispensing tip mounting position, the aspirating and discharging positions for the sample and reagent to be dispensed, and the dispensing tip disposal position. Is also controlled by the control unit 1.
- Container container 3 made of conductive material. It contains a liquid container 12 made of a non-conductive material such as glass or plastic.
- the dispensing probe 1 and the container container 33 are connected to a capacitance measuring unit 9, and the liquid level determining unit 10 connected to the control unit 7 is connected to the capacitance measuring unit 9.
- the dispensing probe 1 is lowered by the dispensing probe vertical movement mechanism 5 and the conductive dispensing tip 2 contacts the liquid to be dispensed in the liquid container 12, the conductive dispensing probe 1 and the container container 3 The capacitance between 3 and changes.
- the capacitance measuring unit 9 measures the capacitance
- the liquid level determination unit 10 detects a change in the capacitance, and sends the detected change to the control unit as a liquid level detection signal.
- the liquid to be dispensed is sucked into the dispensing tip 2 by the operation of the syringe 4.
- the liquid to be dispensed sucked into the dispensing tip 2 is then discharged from the dispensing tip 2 by the operation of the syringe 4.
- a liquid container 8 is provided for containing a non-reactive liquid such as water that does not react with the liquid to be dispensed.
- the dispensing tip 2 contains air inside the dispensing tip 2 before the liquid to be dispensed is sucked. Water, which is a non-reactive liquid, is sucked from the liquid container 8 into the dispensing tip 2 to increase the relative humidity of the liquid.
- FIG. 2 shows an operation flow of the dispensing probe 1 before the liquid to be dispensed is sucked into the dispensing tip 2.
- the dispensing tip 2 is attached to the dispensing probe 1 at a chip attachment position (described later) (step 101).
- the dispensing probe 1 having the dispensing tip 2 moves onto the liquid container 8 provided on the device (step 102).
- the liquid container 8 may be installed below the chip mounting position. Next, the tip of the dispensing tube 2 is lowered into the liquid container 8 by the dispensing probe vertical movement mechanism 5. This descending operation is continued until the liquid level is detected in the same manner as when sucking the liquid to be dispensed, and stops when the liquid level is detected (step 103).
- a predetermined amount of water, which is a non-reactive liquid, in the liquid container 8 is sucked into the dispensing tip 2 held by the dispensing probe 1 by the operation of the syringe 4 (step 104).
- the amount of water to be sucked is, for example, 41.
- the dispensing probe 1 rises (step 105), and slowly sucks air (step 106). For example, 30 l of air is sucked in at a rate of 30 l / s. As a result, the water sucked into the dispensing tip 2 becomes a layer having a thickness of less than 1 mm. In addition, the diameter of the upper surface of the sucked water is 2.7 mm as an example.
- step 107 Bow Continue to suck in air quickly (step 107). For example, 501 air is sucked in at a rate of 15 ⁇ zl / s. As a result, the water layer stretched in the dispensing tip 2 pops out and becomes water droplets or mist. By introducing this process, evaporation of the water sucked into the dispensing tip 2 is promoted, and the relative humidity of the air in the dispensing tip 2 can be efficiently increased and stabilized.
- step 104 the dispensing probe 1 moves to the liquid suction position and sucks the liquid to be dispensed in the liquid container 12.
- the amount of liquid to be dispensed in the dispensing tip 2 becomes negligibly small, and an accurate amount of liquid is dispensed without being substantially affected by changes in environmental temperature and humidity. It can be performed.
- FIG. 3 shows an embodiment of an automatic analyzer to which the liquid dispensing device according to the present invention is applied.
- the plurality of liquid containers 20 are arranged in three rows in a circle on the reagent disk 21, and the reagent disk 21 is rotated by the motor.
- the plurality of reaction vessels 22 are arranged in a circle on a thermostat 23, and the thermostat 23 is rotated by a motor.
- the reaction vessel 22 is moved from the reaction vessel installation position 24 to the reagent dispensing position 26, the sample dispensing position 25, and the reaction liquid suction position 27 by the rotation of the thermostatic bath 23.
- the reagent dispensing probe 32 can be moved by a motor between the reagent suction position and the reagent dispensing position 26.
- Reagent dispensing positions are liquid container 20 dispensing position 3 3 on the outer row, liquid container 20 dispensing position 3 3 b on the middle row and liquid container 20 dispensing position 3 on the inner row. It consists of 3 c.
- the sample dispensing probe 1 can be moved between the sample suction position 29, the sample dispensing position 25, the non-reactive liquid suction position 45, the dispensing tube mounting position 55, and the dispensing chip discarding position 41 by motor.
- a liquid container 8 as shown in FIG. 1 is arranged at the non-reactive liquid suction position 45, and the container is filled with a non-reactive liquid such as water that does not react with the sample.
- the non-reactive liquid suction position 45 may be matched with the dispensing tip mounting position 55. In this case, the liquid container 8 for holding the non-reaction liquid is installed below the dispensing tip mounting position 55.
- the sample rack 30 is drawn into the drop-in line 51 from the transport line 3 and is held by the sample rack, and the sample in the liquid container 12 positioned at the sample suction position 29 is dispensed at the sample dispensing position 2.
- the disposable dispensing tip 2 is attached to the tip of the sample dispensing probe 1.
- This disposable dispensing tip 2 is of course removable.
- the paper 34 can be moved by a motor between the reaction solution suction position [position 27, buffer solution suction position 35, and flow cell internal washing position 36]. Further, the sieve 34 has a function of sending the reaction solution to the flow cell in the detection unit 37 via the tube.
- the dispensing tip and reaction vessel transfer mechanism 38 reacts the disposable dispensing tip 2 from the dispensing tip storage rack 39 to the dispensing tip mounting position 55 and the reaction vessel 22 from the reaction vessel storage rack 40. Transfer to container location 24.
- the reagent dispensing probe 32 and the paper 34 wash their nozzles at their respective washing positions (not shown).
- sample dispensing probe 1 the reagent dispensing probe 32, and the sipper 34 can each be moved up and down at the position where they move and stop.
- the dispensing tip and reaction vessel transfer mechanism 38 moves the disposable dispensing tip 2 from the dispensing tip storage rack 39 to the dispensing tip mounting position 55 and reacts.
- the container 22 is transferred from the reaction container storage rack 40 to the reaction container installation position 24.
- the sample dispensing probe 1 is moved to the dispensing tip mounting position 55, where the disposable dispensing tip 2 is mounted on the tip of the sample dispensing probe 1 (101).
- the sample dispensing probe 1 having the dispensing tip 2 moves to the non-reactive liquid suction position 45 (102), where the dispensing probe 2 moves the liquid of the non-reactive liquid in the liquid container 8 into water.
- the reaction container 22 installed at the reaction container installation position 24 is moved to the reagent suction position 26.
- the reagent dispensing probe 32 moves to the reagent dispensing position 33a, 33b or 33c, where it descends and aspirates the reagent therein, and then rises.
- Reagent dispensing probe 3 2 is aspirated reagent Is moved to the reagent dispensing position 26 and is discharged into the reaction vessel 22 and rises.
- the reaction container 22 is moved to the sample dispensing position 25.
- sample to be dispensed to dispensing tip 2 of sample dispensing probe 1 is dispensed (109), and then sample dispensing probe 1 dispenses. Move to dispensing tip discarding position 4 1 (1 10) and discard dispensing tip 2.
- the sample dispensing probe 1 moves to the sample suction position 29 (1900), and descends until the liquid surface of the sample to be dispensed in the liquid container 12 at that position is detected ( 109 1), the sample is sucked into the dispensing tip 2 attached to the tip of the sample dispensing probe 1 (1092).
- the sample dispensing probe 1 is raised to its original height (1093).
- the sample dispensing probe 1 moves to the sample dispensing position 25 (1094), descends by a predetermined amount (1095), and is moved to the sample dispensing position 25, and the reagent is dispensed.
- the discharged reaction vessel 22 is discharged (1096).
- the sample probe 1 descends again, and aspirates the mixture of the sample and the reagent in the reaction vessel 22 at the sample dispensing position 25 again into the dispensing tip 2 at the tip thereof (1977), and The sucked mixture is discharged again from the dispensing tip 2 (1098), and rises to the original height. As a result, the sample and the reagent are stirred, and the reaction is accelerated. Thereafter, the sample dispensing probe 1 is moved to the dispensing tip discarding position 41 (110), and the dispensing tip 2 at the tip thereof is discarded here (111).
- the fixed time in step 108 means the time from inhaling air to the dispensing tip 2 of the sample dispensing probe 1 until aspirating the sample.This time is related to Figs. 1 and 2. Same as described time.
- the reaction vessel 22 containing the reaction solution is moved to the reaction solution suction position 27, where the paper 34 sucks the reaction solution, and further moves to the buffer solution suction position 35 to suck the buffer solution. Transfer to the flow cell in the detection unit 37 via the tube. Chemiluminescence measurement is performed on the reaction solution transferred to the flow cell, and the analysis results of the immunological analysis items are obtained. Thereafter, the sippers 34 are moved to the flow cell internal cleaning position 36, where they aspirate the flow cell internal cleaning liquid, and Flow through the tube to the flow cell, and wash the port cell.
- the disposable dispensing tip is attached to the tip of the sample dispensing probe, and the non-reactive liquid is sucked into the dispensing tip prior to aspirating the sample.
- a non-reactive liquid may be aspirated on the tip of the reagent dispensing probe, and the dispensing tip may also aspirate the non-reactive liquid prior to aspirating the reagent.
- Table 1 shows the experimental data on the accuracy and reproducibility of liquid dispensing at room temperature of 32 ° C and humidity of 20%, where evaporation is likely to occur.
- Comparative Example 1 is an example in which 50 ⁇ 1 of water is sucked and a sample is sucked after discharging
- Comparative Example 2 is a sample of 41 inhaled water, sucked in air 301 and aspirated for 2 seconds, and then aspirated the sample.
- air was drawn 30 31 / s at a speed of 30 ⁇ 1 / s, then 500 51 / s at a speed of 50 11 / s. Examples of suctioning, waiting for 2 seconds, and then aspirating the sample are shown below. Both are examples of dispensing 10 times.
- Accuracy means (average of actual dispensed sample volume Z sample volume to be dispensed) X 100, and reproducibility is ( The standard deviation of the sample volume actually dispensed Z means the average value of the sample volume actually dispensed) X100. From Table 1, it is clear that the accuracy of dispensing was improved from 87% in Comparative Example 1 and 92% in Comparative Example 2 to 97% in the present invention example. . In addition, the reproducibility of dispensing was 4.3% in Comparative Example 1 and 1.5% in Comparative Example 2, but is clearly improved to 0.6% in the present invention.
- the sample and the reagent in the same dispensing tip into which the sample and the reagent have been dispensed may be re-aspirated and discharged.
- the method of Comparative Example 2 has a disadvantage that water sucked into the dispensing tip is mixed into the mixture of the reagent and the sample.
- mist is generated in the dispensing tip or a very small water droplet is formed at the upper end of the dispensing tip, so that ice is substantially mixed in the mixture of the reagent and the sample. And stable reproducibility can be obtained.
- the influence of the change in the temperature and humidity of the environment on the liquid dispensed amount is reduced.
Description
Claims
Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/JP2000/004390 WO2002003078A1 (fr) | 2000-06-30 | 2000-06-30 | Procede et dispositif de distribution de liquide |
US10/311,805 US7439076B1 (en) | 2000-06-30 | 2000-06-30 | Liquid dispensing method and device |
CNB008197148A CN1193234C (zh) | 2000-06-30 | 2000-06-30 | 液体分注方法及装置 |
JP2002508088A JP4509473B2 (ja) | 2000-06-30 | 2000-06-30 | 液体分注方法および装置 |
EP00942442A EP1306675A4 (en) | 2000-06-30 | 2000-06-30 | LIQUID DISPENSING METHOD AND DEVICE |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/JP2000/004390 WO2002003078A1 (fr) | 2000-06-30 | 2000-06-30 | Procede et dispositif de distribution de liquide |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2002003078A1 true WO2002003078A1 (fr) | 2002-01-10 |
Family
ID=11736211
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/JP2000/004390 WO2002003078A1 (fr) | 2000-06-30 | 2000-06-30 | Procede et dispositif de distribution de liquide |
Country Status (5)
Country | Link |
---|---|
US (1) | US7439076B1 (ja) |
EP (1) | EP1306675A4 (ja) |
JP (1) | JP4509473B2 (ja) |
CN (1) | CN1193234C (ja) |
WO (1) | WO2002003078A1 (ja) |
Cited By (3)
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JP2003302412A (ja) * | 2002-04-10 | 2003-10-24 | Sysmex Corp | 試料分析装置とその液体吸引装置およびピペット洗浄装置 |
JP2010256200A (ja) * | 2009-04-27 | 2010-11-11 | Aloka Co Ltd | 分注装置 |
JP2016004022A (ja) * | 2014-06-19 | 2016-01-12 | コニカミノルタ株式会社 | 検出装置、検出方法および検出チップ |
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CN1329734C (zh) * | 2005-09-07 | 2007-08-01 | 杭州电子科技大学 | 一种微量试剂加给检测装置 |
JP4753770B2 (ja) * | 2006-04-06 | 2011-08-24 | ベックマン コールター, インコーポレイテッド | 分注装置における配管内の気泡の有無判定方法および分注装置 |
US9103782B2 (en) | 2008-12-02 | 2015-08-11 | Malvern Instruments Incorporated | Automatic isothermal titration microcalorimeter apparatus and method of use |
WO2015092844A1 (ja) * | 2013-12-16 | 2015-06-25 | 株式会社島津製作所 | 液採取装置及びその液採取装置を備えた自動分析装置 |
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WO2001003848A1 (en) * | 1999-07-13 | 2001-01-18 | The Texas A & M University System | Pneumatic nebulizing interface, method for making and using same and instruments including same |
JP3750460B2 (ja) * | 2000-02-18 | 2006-03-01 | 日立工機株式会社 | 分注装置及び分注方法 |
US6352673B1 (en) * | 2000-03-09 | 2002-03-05 | Rainin Instrument | Ergonomic return springless manual air displacement pipette |
US6709872B1 (en) * | 2000-05-02 | 2004-03-23 | Irm Llc | Method and apparatus for dispensing low nanoliter volumes of liquid while minimizing waste |
JP2002001092A (ja) * | 2000-06-22 | 2002-01-08 | Shimadzu Corp | 排液装置 |
US20030013200A1 (en) * | 2001-07-12 | 2003-01-16 | Su-Cheng Pai | Liquid sample take-up device |
JP2004325117A (ja) * | 2003-04-22 | 2004-11-18 | Olympus Corp | 液体分注装置および分注ヘッドの洗浄方法 |
US6805175B1 (en) * | 2003-06-12 | 2004-10-19 | Symyx Technologies, Inc. | Powder transfer method and apparatus |
US7134459B2 (en) * | 2003-06-12 | 2006-11-14 | Symyx Technologies, Inc. | Methods and apparatus for mixing powdered samples |
-
2000
- 2000-06-30 EP EP00942442A patent/EP1306675A4/en not_active Withdrawn
- 2000-06-30 US US10/311,805 patent/US7439076B1/en not_active Expired - Fee Related
- 2000-06-30 CN CNB008197148A patent/CN1193234C/zh not_active Expired - Fee Related
- 2000-06-30 WO PCT/JP2000/004390 patent/WO2002003078A1/ja active IP Right Grant
- 2000-06-30 JP JP2002508088A patent/JP4509473B2/ja not_active Expired - Fee Related
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JPS63169565A (ja) * | 1987-01-07 | 1988-07-13 | Hitachi Ltd | 試料分注方法 |
JPH04274765A (ja) * | 1991-03-01 | 1992-09-30 | Mitsubishi Kasei Eng Co | 試料分注装置における分注制御方法 |
JPH05312757A (ja) * | 1992-05-06 | 1993-11-22 | Mitsubishi Heavy Ind Ltd | ゼータ電位測定セル |
JPH10123026A (ja) * | 1996-10-21 | 1998-05-15 | Aloka Co Ltd | 試料吸引方法および試料吸引制御装置 |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2003302412A (ja) * | 2002-04-10 | 2003-10-24 | Sysmex Corp | 試料分析装置とその液体吸引装置およびピペット洗浄装置 |
JP2010256200A (ja) * | 2009-04-27 | 2010-11-11 | Aloka Co Ltd | 分注装置 |
JP2016004022A (ja) * | 2014-06-19 | 2016-01-12 | コニカミノルタ株式会社 | 検出装置、検出方法および検出チップ |
Also Published As
Publication number | Publication date |
---|---|
US7439076B1 (en) | 2008-10-21 |
EP1306675A4 (en) | 2010-07-28 |
CN1193234C (zh) | 2005-03-16 |
EP1306675A1 (en) | 2003-05-02 |
JP4509473B2 (ja) | 2010-07-21 |
CN1454316A (zh) | 2003-11-05 |
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