US20200362051A1 - Molecules that bind to cd137 and psma - Google Patents

Molecules that bind to cd137 and psma Download PDF

Info

Publication number
US20200362051A1
US20200362051A1 US16/763,063 US201816763063A US2020362051A1 US 20200362051 A1 US20200362051 A1 US 20200362051A1 US 201816763063 A US201816763063 A US 201816763063A US 2020362051 A1 US2020362051 A1 US 2020362051A1
Authority
US
United States
Prior art keywords
seq
binding molecule
sequence
molecule according
domain antibody
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
US16/763,063
Other languages
English (en)
Inventor
Verena BRUCKLACHER-WALDERT
Carolyn Edwards
James Legg
Jayesh MAJITHIYA
Christine Rossant
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Crescendo Biologics Ltd
Original Assignee
Crescendo Biologics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB1718734.5A external-priority patent/GB201718734D0/en
Priority claimed from GBGB1718735.2A external-priority patent/GB201718735D0/en
Priority claimed from GBGB1808589.4A external-priority patent/GB201808589D0/en
Application filed by Crescendo Biologics Ltd filed Critical Crescendo Biologics Ltd
Assigned to CRESCENDO BIOLOGICS LIMITED reassignment CRESCENDO BIOLOGICS LIMITED ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: MAJITHIYA, Jayesh, BRUCKLACHER-WALDERT, Verena, ROSSANT, Christine, EDWARDS, Carolyn, LEGG, James
Publication of US20200362051A1 publication Critical patent/US20200362051A1/en
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2878—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the NGF-receptor/TNF-receptor superfamily, e.g. CD27, CD30, CD40, CD95
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2896—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against molecules with a "CD"-designation, not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
    • C07K16/3069—Reproductive system, e.g. ovaria, uterus, testes, prostate
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/565—Complementarity determining region [CDR]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/569—Single domain, e.g. dAb, sdAb, VHH, VNAR or nanobody®
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/75—Agonist effect on antigen
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/76—Antagonist effect on antigen, e.g. neutralization or inhibition of binding
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/77—Internalization into the cell
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/92—Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value

Definitions

  • CD137 (4-1BB, TNFRS9) is a type 1 transmembrane glycoprotein belonging to the TNF receptor superfamily. It was originally cloned by Kwon et al (1989) from the cDNA of activated murine T cells. It has subsequently been shown to have a broad immune cell expression pattern found on T cells, B cells, NK and NK T cells, dendritic cells (DC), macrophages, neutrophils and eosinophils. Expression has also been reported on non-haematopoetic cells, for example epithelial, endothelial and smooth muscle cells and on tumour cell lines. CD137 expression is mainly activation induced, although low level constitutive expression has been demonstrated on some cell types including Tregs and DC's.
  • the 255 amino acid human CD137 protein (Genbank accession NP_001552) consists of a 17 amino acid signal peptide sequence, an extracellular region containing four cysteine rich domains, a 27 amino acid transmembrane region and a short 42 amino acid intracellular domain. It exists as both a monomer and dimer on the cell surface.
  • the main ligand for CD137 is CD137 ligand (CD137L, 4-1BB-L, TNFS9), although interactions with galectin-9 which facilitates receptor aggregation (Madireddi et al 2014) and matrix proteins such as fibronectin (Chalupny et al, 1992) have also been reported.
  • CD137 ligand is predominantly expressed on activated antigen presenting cells such as dendritic cells, B-cells and macrophages.
  • Co-stimulatory TNFR family members such as CD137, CD27, OX40 (CD134), HVEM, CD30, and GITR are involved in sustaining the T cell responses after initial T-cell activation.
  • CD137 acts as a costimulatory receptor that modulates T-cell receptor (TCR) mediated signalling.
  • TCR T-cell receptor
  • Ligation of CD137 together with TCR activation promotes proliferation, cytokine production, and inhibits apoptosis through induction of anti-apoptotic B-cell lymphoma-extra large (Bcl-xl) and B-cell lymphoma 2 (Bcl-2) pathways.
  • CD137 Cross-linking of CD137 on NK cells has been shown to stimulate IFN-gamma secretion and proliferation.
  • Dendritic cell responses to CD137 stimulation include enhanced maturation and antigen presentation and secretion of cytokines IL-6, IL12—and IL-27 and enzymes such as indoleamine-2,3-dioxygenase (IDO) which can modulate T-cell function.
  • CD137 can also upregulate intercellular adhesion molecule 1 (ICAM1) and vascular cell adhesion molecule 1 (VCAM1) on tumor vascular endothelium, thus inducing effector cell migration and retention of the activated T-cells in the tumor microenvironment.
  • IDM1 intercellular adhesion molecule 1
  • VCAM1 vascular cell adhesion molecule 1
  • Cross linking of CD137 by anti CD137 antibodies has been shown to have potent anti-tumour effects in vivo in a number of models including sarcoma, mastocytoma, glioma, lymphoma, myeloma, and hepatocellular carcinoma.
  • CD8+ cell depletion studies have demonstrated that this effect primarily involves cytolytic T cell expansion and infiltration resulting in tumour cell lysis.
  • contributions of other types of cells such as DCs, NK-cells or CD4+ T-cells have been reported in some tumour models.
  • anti CD137 therapy has been shown to trigger an immunologic memory response and to inhibit autoimmune reactions (reviewed in Vinay et al 2012).
  • Agonistic antibodies targeting co-stimulatory TNFRs have been shown to require engagement of Fc ⁇ Rs (Bulliard et al).
  • Fc ⁇ Rs Bolliard et al.
  • non-targeted clustering via Fc ⁇ Rs may influence the mechanism by which agonistic antibodies act on these targets.
  • Prostate cancer is the most commonly diagnosed non-skin-related malignancy in males in developed countries. It is estimated that one in six males will be diagnosed with prostate cancer.
  • prostate cancer has greatly improved following the use of serum-based markers such as the prostate specific antigen (PSA).
  • PSA prostate specific antigen
  • prostate tumour-associated antigens offer targets for tumour imaging, diagnosis, and targeted therapies.
  • PSMA prostate specific membrane antigen
  • a prostate tumour associated marker is such a target.
  • PSMA is a 750-residue type II transmembrane glycoprotein highly restricted to prostate secretory epithelial cell membranes. It is highly expressed in prostate cancer cells and in nonprostatic solid tumor neovasculature and other solid tumors and expressed at lower levels in other tissues, including healthy prostate, kidney, liver, small intestine, and brain. PSMA expression increases with prostate disease progression and metastasis and its expression level has thus been correlated with tumour aggressiveness. Various immunohistological studies have demonstrated increased PSMA levels in virtually all cases of prostatic carcinoma compared to those levels in benign prostate epithelial cells.
  • PSMA staining is found in all stages of the disease, including prostatic intraepithelial neoplasia, late stage androgen-independent prostate cancer and secondary prostate tumours localized to lymph nodes, bone, soft tissue, and lungs. PSMA is thus widely used as a biomarker for prostate cancer cells.
  • PSMA has a 3-part structure: a 19-amino-acid internal portion, a 24-amino-acid transmembrane portion, and a 707-amino-acid external portion. It forms a noncovalent homodimer that possesses glutamate carboxypeptidase activity based on its ability to process the neuropeptide N-acetylaspartylglutamate and glutamate-conjugated folate derivatives. PSMA is rapidly and efficiently internalized by an endocytic pathway and rapidly recycles back to the membrane.
  • the invention addresses the need for alternative antibody-based treatments for use in the treatment of a cancer.
  • the invention relates to novel binding molecules with specificity for both CD137 and PSMA.
  • the inventors have identified single variable heavy chain domain antibodies that bind to CD137 and inhibit binding of CD137L to CD137. They do not cause CD137 signalling when bound to CD137 in monospecific format, that is without being linked to another moiety that binds a second target. However, when linked to a moiety that binds a tumor specific antigen, the single variable heavy chain domain antibodies elicit CD137 signalling.
  • the single variable heavy chain domain antibodies that bind to CD137 do not induce clustering of the receptor and do not have agonistic activity when bound to CD137 without a binding partner that targets a second antigen, the dual engagement of CD137 and a tumor specific antigen in a bispecific molecule leads to CD137 agonism.
  • the single variable heavy chain domain antibodies that bind to CD137 can therefore be used as a subunit in a multispecific binding molecule that simultaneously engages CD137 and PSMA.
  • Bi- and multispecific molecules described herein bind to CD137 and PSMA and simultaneously engage both targets. This dual engagement results in CD137 activation, thus restricting the site of action to the tumor microenvironment and potentially minimising undesirable effects of existing CD137 therapies.
  • an isolated binding molecule comprising or consisting of a) a single variable heavy chain domain antibody that binds to CD137 and
  • the single variable heavy chain domain antibody that binds to CD137 comprises a CDR1 comprising SEQ ID NO. 1 or a sequence with at least 40% homology thereto, a CDR2 comprising SEQ ID NO. 2 or a sequence with at least 40% homology thereto and a CDR3 comprising SEQ ID NO. 3 or a sequence with at least 40% homology thereto or a CDR1 comprising SEQ ID NO. 425 or a sequence with at least 40% homology thereto, a CDR2 comprising SEQ ID NO. 426 or a sequence with at least 40% homology thereto and a CDR3 comprising SEQ ID NO. 427 or a sequence with at least 40% homology thereto.
  • the single variable heavy chain domain antibody that binds to CD137 comprises human framework regions.
  • the single variable heavy chain domain antibody that binds to CD137 comprises a full length sequence as listed in table 2 or 3 or a sequence with at least 75% homology thereto. In one embodiment, the single variable heavy chain domain antibody that binds to CD137 comprises or consists of one of the following sequences: SEQ ID NO. 4, 312, 428, 852, 856, 860, 864, 868, 872, 876 or 880 or a sequence with at least 50% homology thereto.
  • the moiety that binds to PSMA is selected from an antibody, an antibody fragment, an antibody mimetic, a protein that mimics the natural ligand of CD137 or other polypeptide.
  • said antibody fragment is selected from a Fab, F(ab′)2, Fv, a single chain Fv fragment (scFv), a single domain antibody or fragment thereof.
  • said single domain antibody is a single V H domain antibody.
  • said single V H domain antibody comprises a CDR1 comprising SEQ ID NO. 812 or a sequence with at least 75% homology thereto, a CDR2 comprising SEQ ID NO. 813 or a sequence with at least 75% homology thereto and a CDR3 comprising SEQ ID NO. 814 or a sequence with at least 75% homology thereto or wherein said V H single domain antibody comprises a CDR1 comprising SEQ ID NO. 837 or a sequence with at least 75% homology thereto, a CDR2 comprising SEQ ID NO. 838 or a sequence with at least 75% homology thereto and a CDR3 comprising SEQ ID NO. 839 or a sequence with at least 75% homology thereto.
  • the single variable heavy chain domain antibody that binds to PSMA comprises a full length sequence as listed in table 6 or 7 or a sequence with at least 50% homology thereto.
  • the single variable heavy chain domain antibody moiety that binds to PSMA comprises SEQ ID NO. 815, 816, 817, 818, 819, 820, 821, 822, 823, 824, 825 or 840 or a sequence with at least 50% homology thereto.
  • a single domain antibody 1.1 or 2.1 having one of SEQ ID No. 4, 312, 852, 856, 860, 864, 868, 872, 876, 880 or 428 or a variant t (e.g. a molecules with 1 to 10 or 1 to 20 amino acid substitutions) hereof is linked to a single domain antibody 3.1, 3.8 or 4.1 having SEQ ID No. 815, 822 or 840 respectively.
  • the isolated binding molecule is capable of binding CD137 with an affinity with a Kd of about of at least about 10-6 M, alternatively at least about 10-7 M, alternatively at least about 10-8 M, alternatively at least about 10-9 M, alternatively at least about 10-10 M, alternatively at least about 10-11 M, alternatively at least about 10-12 M, or greater affinity.
  • the isolated binding molecule is capable of binding PSMA with an affinity with a Kd of about of at least about 10-6 M, alternatively at least about 10-7 M, alternatively at least about 10-8 M, alternatively at least about 10-9 M, alternatively at least about 10-10 M, alternatively at least about 10-11 M, alternatively at least about 10-12 M, or greater affinity.
  • the single variable heavy chain domain antibody that binds to CD137 is linked to the moiety that binds PSMA by a peptide linker.
  • said linker is selected from a (G4S)n linker wherein n is 1 to 10.
  • An isolated binding molecule of the invention may comprise a single variable heavy chain domain antibody that binds to CD137 linked to a moiety that binds PSMA.
  • the isolated binding molecule is conjugated to a toxin, enzyme, radioisotope, half-life extending moiety, label, therapeutic molecule or other chemical moiety.
  • said half-life extending moiety is selected from the group consisting of an albumin binding moiety, a transferrin binding moiety, a polyethylene glycol molecule, a recombinant polyethylene glycol molecule, human serum albumin, a fragment of human serum albumin, and an albumin binding peptide or single domain antibody that binds to human serum albumin.
  • the single variable heavy chain domain antibody which binds to human CD137 does not cause CD137 signalling when bound to CD137 as a monospecific entity.
  • the single variable heavy chain domain antibody which binds to human CD137 is obtained or obtainable from a transgenic rodent that expresses a transgene comprising human V, D and J regions.
  • said rodent e.g. a mouse, does not produce functional endogenous light and heavy chains.
  • composition comprising a binding molecule as described herein, e.g. comprising a single variable heavy chain domain antibody as described herein and a pharmaceutical carrier.
  • binding molecule or a pharmaceutical composition as described herein for use in the treatment of disease such as cancer, prostate cancer or a PSMA positive tumor.
  • Also provided is a method for treating cancer comprising administering a therapeutically effective amount of a binding molecule or a pharmaceutical composition as described herein.
  • said cancer is prostate cancer lung cancer, glioblastoma, renal, bladder, testicular, neuroendocrine, colon, and breast cancer.
  • nucleic acid molecule comprising a nucleic acid sequence encoding the binding molecule as described herein.
  • Also provided is a host cell comprising a nucleic acid molecule or a vector as described herein.
  • the host cell is a bacterial, yeast, viral, plant or mammalian cell.
  • Also provided is a method for producing a binding molecule described herein comprising expressing a nucleic acid encoding said binding molecule in a host cell and isolating the binding molecule from the host cell.
  • Also provided is a method for promoting CD8+ T cell expansion, inducing activation of cytotoxic T lymphocytes (CTL) and/or cytokine release comprising administering a binding molecule or a pharmaceutical composition as described herein.
  • CTL cytotoxic T lymphocytes
  • Also provided is an in vivo or in vitro method for reducing human PSMA activity comprising contacting human PSMA with a binding molecule as described above.
  • kits comprising a binding molecule as described herein or a pharmaceutical composition as described herein.
  • binding molecule or a pharmaceutical composition as described above for activating, e.g. simultaneously activating, downstream signalling pathways of CD137 and PSMA.
  • Also provided is a method for dual engagement of CD137 and PSMA comprising administering a binding molecule or a pharmaceutical composition as described herein.
  • FIG. 1 Dual Binding Cell based ELISA.
  • CHO human PSMA expressing cells were seeded onto plates and monovalent V H or bispecific molecules added.
  • CD137huFc was subsequently added and binding detected using anti human Fc-HRP. Only bispecific molecule showed increased binding signal confirming dual target binding.
  • FIG. 2 Activation of CD137 signalling in the Jurkat NF-kB Luciferase Reporter Assay.
  • CHO PSMA cells (B) DU145 PSMA cells or (C) DU145 parental cells (bispecific testing)/media only (antibody testing) were cultured with Jurkat human CD137 NF-kB-luciferase reporter cells.
  • Relative luminescence signal (RLU) was measured as a readout of the luciferase reporter gene activity resulting from CD137 mediated activation of the NF-kB signalling pathway.
  • Monovalent Humabody® V H 1.1 and 2.1 were unable to stimulate a response.
  • Bispecific Humabody® V H stimulated CD137 signalling in a PSMA dependent and concentration dependent manner. The level of response was PSMA expression level dependent with higher maximal response observed in the presence of higher expression of PSMA.
  • Anti CD137 antibody responses were PSMA expression independent. Bispecific molecules in the presence of PSMA expressing cells were able to effectively stimulate CD137 signalling.
  • FIG. 3 Enhancement of cytokine production in T-cells.
  • Human CD8+ T cells were co-cultured with PSMA expressing cells or non-expressing parental cells in the presence of plate bound anti CD3 antibody.
  • Humabody® V H 1.1 monovalent and bispecific molecules Humabody® V H 2.1 monovalent and bispecific molecules, anti CD137 comparator antibody and anti PSMA antibody.
  • Supernatants were harvested after 48 hours and levels of IL-2 determined.
  • A Enhancement of IL-2 responses in the presence of PSMA expressing and non-PSMA expressing cells
  • B IL-2 responses for 3 different T-cell donors in the presence of PSMA expressing cells.
  • C and D Concentration dependence of IL-2 response.
  • E Interferon-gamma production in the presence of PSMA expressing cells.
  • Monovalent Humabody® V H did not stimulate IL2 or IFN-gamma production in the assay.
  • Bispecific molecules in the presence of PSMA expressing cells were able to effectively enhance cytokinelL-2 production.
  • the anti CD137 antibody (soluble/non-cross linked) enhanced IL-2 production in a PSMA independent response.
  • FIG. 4 Mode of action of bispecific molecule. This figure illustrates the mode of action of a binding molecule that binds both CD137 and PSMA, leading to tumor selective T cell agonism.
  • FIG. 5 Enhancement of TNF alpha production.
  • SEB Pre-stimulated PBMCs were treated for 3 days with 1 ng/ml SEB and Humabody constructs 4.1-6GS-1.1, 3.8-6GS-1.1 or a control V H in the presence of either (A) CHO PSMA cells or (B) CHO parent cells and 1 ng/ml SEB. TNF-alpha concentrations (Mean ⁇ standard deviation) were determined from 3 replicate wells.
  • FIG. 6 In vivo experiment: Effect of Humabody® in DU145 PSMA/hu PBMC engrafted NCG Mice Pooled tumour volume data from HuPBMC engrafted NCG mice implanted with DU145 PSMA prostate cell lines.
  • Enzymatic reactions and purification techniques are performed according to manufacturers specifications, as commonly accomplished in the art or as described herein.
  • the nomenclatures used in connection with, and the laboratory procedures and techniques of, analytical chemistry, synthetic organic chemistry, and medicinal and pharmaceutical chemistry described herein are those well-known and commonly used in the art. Standard techniques are used for chemical syntheses, chemical analyses, pharmaceutical preparation, formulation, and delivery, and treatment of patients. Suitable assays to measure the properties as set out above are also described in the examples.
  • antibody as used herein broadly refers to any immunoglobulin (Ig) molecule, or antigen binding portion thereof, comprised of four polypeptide chains, two heavy (H) chains and two light (L) chains, or any functional fragment, mutant, variant, or derivation thereof, which retains the essential epitope binding features of an Ig molecule.
  • Ig immunoglobulin
  • each heavy chain is comprised of a heavy chain variable region or domain (abbreviated herein as HCVR) and a heavy chain constant region.
  • the heavy chain constant region is comprised of three domains, C H 1, C H 2 and C H 3.
  • Each light chain has a light chain variable region or domain (abbreviated herein as LCVR) and a light chain constant region.
  • the light chain constant region is comprised of one domain, C L .
  • the heavy chain and light chain variable regions can be further subdivided into regions of hypervariability, termed complementarity determining regions (CDR), interspersed with regions that are more conserved, termed framework regions (FR).
  • CDR complementarity determining regions
  • FR framework regions
  • Each heavy chain and light chain variable region is composed of three CDRs and four FRs, arranged from amino-terminus to carboxy-terminus in the following order: FR1, CDR1, FR2, CDR2, FR3, CDR3, FR4.
  • Immunoglobulin molecules can be of any type (e.g., IgG, IgE, IgM, IgD, IgA and IgY), class (e.g., IgG 1, IgG2, IgG 3, IgG4, IgAI and IgA2) or subclass.
  • type e.g., IgG, IgE, IgM, IgD, IgA and IgY
  • class e.g., IgG 1, IgG2, IgG 3, IgG4, IgAI and IgA2
  • subclass e.g., IgG 1, IgG2, IgG 3, IgG4, IgAI and IgA2
  • CDR refers to the complementarity-determining region within antibody variable sequences. There are three CDRs in each of the variable regions of the heavy chain and the light chain, which are designated CDR1, CDR2 and CDR3, for each of the variable regions.
  • CDR set refers to a group of three CDRs that occur in a single variable region capable of binding the antigen. The exact boundaries of these CDRs can be defined differently according to different systems known in the art.
  • CDRs The Kabat Complementarity Determining Regions
  • CDRs The Kabat Complementarity Determining Regions
  • Chothia refers instead to the location of the structural loops (Chothia and Lesk J. Mol. Biol. 196:901-917 (1987)).
  • the Kabat numbering system is generally used when referring to a residue in the variable domain (approximately residues 1-107 of the light chain and residues 1-113 of the heavy chain).
  • IMGT ImMunoGeneTics
  • the IMGT numbering scheme is described in Lefranc et al., Dev. Comp. Immunol., 29, 185-203 (2005).
  • Kabat numbering “Kabat definitions” and “Kabat labeling” are used interchangeably herein. These terms, which are recognized in the art, refer to a system of numbering amino acid residues which are more variable (i.e., hypervariable) than other amino acid residues in the heavy and light chain variable regions of an antibody, or an antigen binding portion.
  • a chimeric antibody is a recombinant protein that contains the variable domains including the complementarity determining regions (CDRs) of an antibody derived from one species, preferably a rodent antibody, while the constant domains of the antibody molecule are derived from those of a human antibody.
  • CDRs complementarity determining regions
  • a humanized antibody is a recombinant protein in which the CDRs from an antibody from one species; e.g., a rodent antibody, are transferred from the heavy and light variable chains of the rodent antibody into human heavy and light variable domains (e.g., framework region sequences).
  • the constant domains of the antibody molecule are derived from those of a human antibody.
  • a limited number of framework region amino acid residues from the parent (rodent) antibody may be substituted into the human antibody framework region sequences.
  • antigen binding site refers to the part of the antibody or antibody fragment that comprises the area that specifically binds to an antigen.
  • An antigen binding site may be provided by one or more antibody variable domains.
  • An antigen binding site is typically comprised within the associated V H and V L of an antibody or antibody fragment.
  • An antibody fragment is a portion of an antibody, for example as F(ab′)2, Fab, Fv, scFv, heavy chain, light chain, heavy (V H ), variable light (V L ) chain domain and the like.
  • Functional fragments of a full length antibody retain the target specificity of a full antibody.
  • Recombinant functional antibody fragments such as Fab (Fragment, antibody), scFv (single chain variable chain fragments) and single domain antibodies (dAbs) have therefore been used to develop therapeutics as an alternative to therapeutics based on mAbs.
  • scFv fragments ( ⁇ 25 kDa) consist of the two variable domains, V H and V L .
  • V H and V L domain are non-covalently associated via hydrophobic interaction and tend to dissociate.
  • stable fragments can be engineered by linking the domains with a hydrophilic flexible linker to create a single chain Fv (scFv).
  • the smallest antigen binding fragment is the single variable fragment, namely the single variable heavy (V H ) or single variable light (V L ) chain domain.
  • V H and V L domains respectively are capable of binding to an antigen. Binding to a light chain/heavy chain partner respectively or indeed the presence of other parts of the full antibody is not required for target binding.
  • the antigen-binding entity of an antibody, reduced in size to one single domain is generally referred to as a “single domain antibody” or “single immunoglobulin variable domain”.
  • a single domain antibody ( ⁇ 12 to 15 kDa) thus consists of either the V H or V L domain, but it does not comprise other parts of a full length antibody.
  • Single domain antibodies derived from camelid heavy chain only antibodies that are naturally devoid of light chains as well as single domain antibodies that have a human heavy chain domain have been described (Muyldermans 2001, Holliger 2005).
  • Antigen binding single V H domains have also been identified from, for example, a library of murine V H genes amplified from genomic DNA from the spleens of immunized mice and expressed in E. coli (Ward et al., 1989, Nature 341: 544-546). Ward et al.
  • dAbs for “domain antibodies.”
  • the term “dAb” or “sdAb” generally refers to a single immunoglobulin variable domain (V H , V HH or V L ) polypeptide that specifically binds antigen. Such a molecule only has the V H or V L binding domain respectively, but does not comprise other parts of a full length antibody. Unless otherwise specified, as used herein, the term refers to a single domain antibody that has a V H domain.
  • human single domain antibodies are preferred, primarily because they are not as likely to provoke an immune response when administered to a patient.
  • single domain antibody V H domain antibody
  • single variable domain antibody variable heavy chain domain antibody
  • single variable heavy chain domain antibody single variable heavy chain domain antibody
  • immunoglobulin single variable domain ISV
  • V H domain which retains binding specificity to the antigen in the absence of light chain or other antibody fragments.
  • a single variable heavy chain domain antibody is capable of binding to an antigen in the absence of light chain.
  • a single variable heavy chain domain antibody does not comprise other parts of a full length antibody; it only includes the V H domain.
  • these terms and specifically a single domain antibody specify a binding moiety that is solely made up of the V H domain and does not have other parts of an antibody.
  • the CD137 binding entity illustrated below is a V H single domain antibody and in preferred embodiments, the PSMA binding entity is also a V H single domain antibody.
  • the embodiments relate to isolated binding molecules which comprise or consist of a single variable heavy chain domain antibody/immunoglobulin single variable heavy chain domain which bind a CD137 antigen and also comprise a moiety that binds to PSMA.
  • the single variable heavy chain domain antibody i.e. a V H domain
  • V H domain antibody Human single variable heavy chain domain antibodies
  • Such binding molecules are also termed Humabody® herein. Humabody® is a registered trademark of Crescendo Biologics Ltd.
  • isolated refers to a moiety that is isolated from its natural environment.
  • isolated refers to a single domain antibody or binding molecule that is substantially free of other single domain antibodies or binding molecule, antibodies or antibody fragments.
  • an isolated single domain antibody may be substantially free of other cellular material and/or chemicals.
  • Each V H domain antibody comprises three CDRs and four FRs, arranged from amino-terminus to carboxy-terminus in the following order: FR1-CDR1-FR2-CDR2-FR3-CDR3-FR4.
  • the domain is a human variable heavy chain (V H ) domain with the following formula FR1-CDR1-FR2-CDR2-FR3-CDR3-FR4.
  • V H domain may comprise C or N-terminal extensions.
  • C-terminal extensions can be added to the C-terminal end of a V H domain which terminates with the residues VTVSS (SEQ ID No. 788).
  • the single domain antibodies of the invention comprise C-terminal extensions of from 1 to 50 residues, for example 1 to 10, e.g. 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10, 1-20, 1-30 or 1-40 additional amino acids.
  • the single domain antibodies of the invention comprise additional amino acids of the human C H 1 domain thus that the C terminal end extends into the C H 1 domain.
  • C-terminal extensions may comprise neutral, nonpolar amino acids, such as A, L, V, P, M, G, I, F or W or neutral polar amino acids, such as S or T.
  • C-terminal extensions may also be selected from peptide linkers or tags, e.g. SEQ ID Nos. 790-797.
  • Additional C or N-terminal residues can be peptide linkers that are for example used to conjugate the single domain antibodies of the invention to another moiety, or tags that aid the detection of the molecule.
  • tags are well known in the art and include for, example linker His tags, e.g., hexa-His (HHHHHH, SEQ ID No. 789) or myc tags.
  • the term “homology” or “identity” generally refers to the percentage of amino acid residues in a sequence that are identical with the residues of the reference polypeptide with which it is compared, after aligning the sequences and in some embodiments after introducing gaps, if necessary, to achieve the maximum percentage homology, and not considering any conservative substitutions as part of the sequence identity.
  • the percentage homology between two amino acid sequences is equivalent to the percentage identity between the two sequences.
  • N- or C-terminal extensions, tags or insertions shall be construed as reducing identity or homology. Methods and computer programs for the alignment are well known.
  • the percentage identity between two amino acid sequences can be determined using well known mathematical algorithms.
  • variable domain of the single domain antibodies as described herein is a human variable domain (as used herein V H refers to a human domain), a camelid variable domain (V HH ), a humanised V HH domain, a camelized V H domain, a sequence modified V H or V HH domain.
  • V H refers to a human domain
  • V HH camelid variable domain
  • V HH camelid variable domain
  • the variable domain of the single domain antibodies as described herein is a V H domain.
  • a human V H domain includes a fully human or substantially fully human V H domain.
  • the term human V H domain also includes V H domains that are isolated from heavy chain only antibodies made by transgenic mice expressing fully human immunoglobulin heavy chain loci, in particular in response to an immunisation with an antigen of interest, for example as described in WO2016/062990 and in the examples below.
  • a human V H domain can also include a V H domain that is derived from or based on a human V H domain amino acid or produced from a human V H nucleic acid sequence.
  • human V H domain includes variable heavy chain regions derived from or encoded by human germline immunoglobulin sequences and for example obtained from heavy chain only antibodies produced in transgenic mice expressing fully human V H genes.
  • a substantially human V H domain or V H domain that is derived from or based on a human V H domain may include amino acid residues not encoded by human germline immunoglobulin sequences (e.g., mutations introduced in vitro, e.g. by random or site-specific mutagenesis, or introduced by somatic mutation in vivo).
  • the term “human V H domain” therefore also includes a substantially human V H domain, i.e. human V H domain wherein one or more amino acid residue has been modified, for example to remove sequence liabilities.
  • a substantially human V H domain the V H domain may include up to 10, for example 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 or up to 20 amino acid modifications compared to a germline human sequence.
  • human V H domain or “substantially human V H domain”, as used herein, is not intended to include antibodies in which CDR sequences derived from the germline of another mammalian species, such as a mouse, have been grafted onto human framework sequences.
  • the term “human V H domain”, as used herein, is also not intended to include camelized V H domains, that is human V H domains that have been specifically modified, for example in vitro by conventional mutagenesis methods to select predetermined positions in the V H domains sequence and introduce one or more point mutation at the predetermined position to change one or more predetermined residue to a specific residue that can be found in a camelid V HH domain.
  • KD refers to the “equilibrium dissociation constant” and refers to the value obtained in a titration measurement at equilibrium, or by dividing the dissociation rate constant (Koff) by the association rate constant (Kon).
  • KA refers to the affinity constant.
  • the association rate constant, the dissociation rate constant and the equilibrium dissociation constant are used to represent the binding affinity of an antibody to an antigen. Methods for determining association and dissociation rate constants are well known in the art. Using fluorescence-based techniques offers high sensitivity and the ability to examine samples in physiological buffers at equilibrium. Other experimental approaches and instruments such as a BIAcore® assay can be used.
  • binding or “specifically binds to” or is “specific for” a particular polypeptide or an epitope on a particular polypeptide target as used in this disclosure can be exhibited, for example, by a molecule having a KD for the target of at least about 10-6 M, alternatively at least about 10-7 M, alternatively at least about 10-8 M, alternatively at least about 10-9 M, alternatively at least about 10-10 M, alternatively at least about 10-11 M, alternatively at least about 10-12 M, or greater affinity.
  • the term “specific binding” refers to binding where a molecule binds to a particular polypeptide or epitope on a particular polypeptide without substantially binding to any other polypeptide or polypeptide epitope.
  • binding molecule that comprises a V H single domain antibody that is a variant of any of the single V H domain antibodies described herein having one or more amino acid substitutions, deletions, insertions or other modifications, and which retains a biological function of the single domain antibody.
  • variant V H single domain antibody can be sequence engineered.
  • Modifications may include one or more substitution, deletion or insertion of one or more codons encoding the single domain antibody or polypeptide that results in a change in the amino acid sequence as compared with the native sequence V H single domain antibody or polypeptide.
  • Amino acid substitutions can be the result of replacing one amino acid with another amino acid having similar structural and/or chemical properties, such as the replacement of a leucine with a serine, i.e., conservative amino acid replacements.
  • Insertions or deletions may optionally be in the range of about 1 to 25, for example 1 to 5, 1 to 10, 1 to 15 or 1 to 20 amino acids, for example 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 amino acids.
  • a variant of a V H single domain antibody described herein has at least 50%, for example at least 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 8 0%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence homology to the non-variant molecule, preferably at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence homology.
  • the modification is a conservative sequence modification.
  • conservative sequence modifications is intended to refer to amino acid modifications that do not significantly affect or alter the binding characteristics of the antibody containing the amino acid sequence. Such conservative modifications include amino acid substitutions, additions and deletions. Modifications can be introduced into an sdAb of the invention by standard techniques known in the art, such as site-directed mutagenesis and PCR-mediated mutagenesis. Conservative amino acid substitutions are ones in which the amino acid residue is replaced with an amino acid residue having a similar side chain. Families of amino acid residues having similar side chains have been defined in the art.
  • amino acids with basic side chains e.g., lysine, arginine, histidine
  • acidic side chains e.g., aspartic acid, glutamic acid
  • uncharged polar side chains e.g., glycine, asparagine, glutamine, serine, threonine, tyrosine, cysteine, tryptophan
  • nonpolar side chains e.g., alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine
  • beta-branched side chains e.g., threonine, valine, isoleucine
  • aromatic side chains e.g., tyrosine, phenylalanine, tryptophan, histidine
  • one or more amino acid residues within the CDR regions of a single domain antibody of the invention can be replaced with other amino acid residues from the same side chain family and the altered antibody can be tested for retained function (i.e., CD137 binding) using the functional assays described herein.
  • these amino acid changes can typically be made without altering the biological activity, function, or other desired property of the polypeptide, such as its affinity or its specificity for antigen. In some instances these changes are made to improve the affinity of the antibody, e.g., single V H domain antibody, for its target antigen.
  • single amino acid substitutions in nonessential regions of a polypeptide do not substantially alter biological activity.
  • substitutions of amino acids that are similar in structure or function are less likely to disrupt the polypeptides' biological activity.
  • Table 1 Abbreviations for the amino acid residues that comprise polypeptides and peptides described herein, and conservative substitutions for these amino acid residues are shown in Table 1 below.
  • the binding molecule includes a V H single domain antibody that is a variant of a single domain antibody selected from those shown in Tables 2, 3 and 4 that comprises one or more sequence modification and has improvements in one or more of a property such as binding affinity, specificity, thermostability, expression level, effector function, glycosylation, reduced immunogenicity, or solubility as compared to the unmodified single domain antibody.
  • modifications can be made to decrease the immunogenicity of the single domain antibody.
  • one approach is to revert one or more framework residues to the corresponding human germline sequence.
  • a single domain antibody that has undergone somatic mutation may contain framework residues that differ from the germline sequence from which the single domain antibody is derived. Such residues can be identified by comparing the single domain antibody framework sequences to the germline sequences from which the single domain antibody is derived. In one embodiment, all framework residues are germline sequence.
  • the somatic mutations can be “backmutated” to the germline sequence by, for example, site-directed mutagenesis or PCR-mediated mutagenesis.
  • Another type of framework modification involves mutating one or more residues within the framework region, or even within one or more CDR regions, to remove T cell epitopes to thereby reduce the potential immunogenicity of the antibody.
  • the glycosylation is modified.
  • an aglycoslated antibody can be made (i.e., the antibody lacks glycosylation).
  • Glycosylation can be altered to, for example, increase the affinity of the antibody for antigen.
  • carbohydrate modifications can be accomplished by, for example, altering one or more sites of glycosylation within the antibody sequence.
  • one or more amino acid substitutions can be made that result in elimination of one or more variable region framework glycosylation sites to thereby eliminate glycosylation at that site.
  • Such aglycosylation may increase the affinity of the antibody for the antigen.
  • the one or more substitution is in the CDR1, 2 or 3 region.
  • the one or more substitution is in the framework region.
  • epitopes within protein antigens can be formed both from contiguous amino acids (usually a linear epitope) or non-contiguous amino acids juxtaposed by tertiary folding of the protein (usually a conformational epitope).
  • Epitopes formed from contiguous amino acids are typically, but not always, retained on exposure to denaturing solvents, whereas epitopes formed by tertiary folding are typically lost on treatment with denaturing solvents.
  • An epitope typically includes at least 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14 or 15 amino acids in a unique spatial conformation.
  • Methods for determining what epitopes are bound by a given antibody or antibody fragment are well known in the art and include, for example, immunoblotting and immunoprecipitation assays, wherein overlapping or contiguous peptides from are tested for reactivity with a given antibody or antibody fragment.
  • An antibody binds “essentially the same epitope” as a reference antibody, when the two antibodies recognize identical or sterically overlapping epitopes.
  • the most widely used and rapid methods for determining whether two epitopes bind to identical or sterically overlapping epitopes are competition assays, which can be configured in different formats, using either labelled antigen or labelled antibody.
  • the inventors have surprisingly identified single variable heavy chain domain antibodies that, when targeted to CD137 in a monospecific format, that is without being linked to another moiety specific to a second antigen, bind specifically to CD137, but do not induce clustering of the CD137 receptor. Binding of the single variable heavy chain domain antibodies described herein in a monovalent or monospecific format does therefore not activate CD137 signalling and does not lead to CD137 signalling.
  • Binding of the single variable heavy chain domain antibodies described herein does not agonise CD137 signalling unless they are provided together with another moiety specific to a second antigen, for example as a bispecific fusion protein wherein a single variable heavy chain domain antibody described herein is linked to a moiety that binds to a tumor specific antigen, for example a single variable heavy chain domain antibody that binds to a tumor specific antigen.
  • a single variable heavy chain domain antibody as described herein is provided as part of a binding molecule, for example as fusion protein together with a moiety that binds to PSMA, such as a single variable heavy chain domain antibody that binds to PSMA
  • binding to CD137 and the target moiety results in clustering of the CD137 receptor and CD137 signalling.
  • Induction of CD137 signalling thus requires dual engagement of both targets, i.e. CD137 and PSMA.
  • Only simultaneous engagement of both targets by the bispecific molecule results in CD137 activation.
  • Target specific activation in the vicinity of the tumor potentially avoids systemic CD137 effects leading to uncontrollable side effects.
  • the binding molecules effectively engage CD137 on the surface of cells through mechanisms other than binding to Fc-receptors thus also avoiding unwanted liver toxicity. Simultaneously, they engage cells that express PSMA.
  • binding molecule that binds to both CD137 and PSMA.
  • binding molecule and “binding agent” are used interchangeably herein.
  • a binding molecule as used herein refers to a binding molecule that specifically binds at least two targets, i.e. CD137 and PSMA, wherein one subunit/entity/moiety that binds to CD137 is conjugated/linked a second subunit/entity/moiety that binds PSMA.
  • the binding molecule is a fusion protein wherein one polypeptide that binds to CD137 is conjugated/linked to a second polypeptide that binds to PSMA.
  • the invention relates to an isolated multispecific binding molecule that binds to both CD137 and PSMA and comprises a single variable heavy chain domain antibody that binds to CD137.
  • the single variable heavy chain domain antibody that binds to CD137 is as described herein.
  • the properties of the multispecific binding molecules of the invention can be exploited in therapeutic methods and uses as well as in pharmaceutical formulations as described herein.
  • the invention relates an isolated binding molecule comprising or consisting of a) a single variable heavy chain domain antibody that binds to CD137 and
  • the entity that binds to CD137 is not a full antibody that comprises light and heavy chains, but a single variable heavy chain domain, i.e. a V H domain only.
  • the single variable heavy chain domain antibody that binds to CD137 is selected from one of the single variable heavy chain domain antibodies that bind to CD137 having a SEQ ID No. as described herein as listed in the tables below (tables 2 and 3) wherein the CDRs are defined according to Kabat.
  • the single variable heavy chain domain antibody that binds to CD137 and the moiety that binds to PSMA are linked, for example by a peptide linker.
  • the single variable heavy chain domain antibody that binds to CD137 can be linked at its N or C terminus to the moiety that binds to PSMA.
  • the moiety that binds PSMA can be selected from an antibody, antibody mimetic, antibody scaffold, antibody fragment or other polypeptide.
  • An antibody fragment can be selected from a portion of an antibody, for example a F(ab′)2, Fab, Fv, scFv, heavy chain, light chain, heavy or variable light chain domain heavy or variable light chain domain, or part thereof, such as a CDR.
  • the entity that binds PSMA is a single variable heavy chain domain antibody that binds to PSMA, such as a human V H domain antibody.
  • the single variable heavy chain domain antibody that binds to PSMA is selected from one of the single variable heavy chain domain antibodies that bind to PSMA having a SEQ ID No. as described herein and in the tables below.
  • binding molecule comprising or consisting of
  • the binding molecule has two single variable heavy chain domains, one that binds to CD137 and one that binds to PSMA. No other domains/chains of a full antibody are present.
  • the single variable heavy chain domain antibody that binds CD137 and the single variable heavy chain domain antibody that binds PSMA is a human V H domain antibody.
  • binding molecule comprising a single variable heavy chain domain antibody that binds to CD137, for example having a SEQ ID No. as described herein (e.g. as set out in table 2 or 3), linked to another moiety that binds to PSMA, for example having a SEQ ID No. as described herein wherein the binding molecule exhibits one or more of the following properties:
  • (a) binds to human CD137 with a KD as measured in the examples; (b) inhibits the interaction between human CD137 ligand and human CD137 expressed on the surface of cells. This can be measured as shown in example 4; (c) does not bind to mouse CD137; (d) binds to cells expressing CD137 and simultaneously binds to cells expressing PSMA. This can be measured as shown in example 5; (e) increases reporter gene activity. This can be measured as shown in example 5; (f) inhibits tumor cell growth in vivo; (g) promotes CD8+ T cell expansion; (h) induces activation of cytotoxic T lymphocytes (CTL); (i) stimulates IL-2 production from CD8+ cells.
  • CTL cytotoxic T lymphocytes
  • the binding molecule exhibits more than 1 of the properties above, for example 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21 or all of the properties selected from the above list, including any combination of properties.
  • the binding agent inhibits the interaction between human CD137 ligand and human CD137 expressed on the surface of cells.
  • the binding molecule is a fusion protein comprising at least two subunits, i.e. a CD137-binding subunit fused to a PSMA-binding subunit wherein the CD137-binding subunit is a single variable heavy chain domain antibody that binds to CD137.
  • the binding molecule is a fusion protein comprising a single variable heavy chain domain antibody that binds to CD137 linked to a single variable heavy chain domain antibody that binds to PSMA.
  • the binding agent is multispecific, for example bispecific or trispecific.
  • a bispecific molecule binds to 2 different targets.
  • a trispecific molecule binds to 2 different targets.
  • a monovalent molecule has one binding entity.
  • a bivalent molecule has two binding entities which bind to the same or different target.
  • the binding molecule comprises a first V H single domain antibody that binds to CD137 (V H (A)) and a second V H single domain antibody (V H (B)) that binds to PSMA and thus has the following formula: V H (A)-L-V H (B).
  • V H (A) is conjugated to V H (B), that is linked, for example with a peptide linker.
  • L denotes a linker.
  • Each V H comprises CDR and FR regions.
  • the binding molecule may have the following formula: FR1(A)-CDR1(A)-FR2(A)-CDR2(A)-FR3(A)-CDR3(A)-FR4(A)-L-FR1(B)-CDR1(B)-FR2(B)-CDR2(B)-FR3(B)-CDR3(B)-FR4(B).
  • the order of the single V H domains A and B is not particularly limited, so that, within a polypeptide of the invention, single variable domain A may be located N-terminally and single variable domain B may be located C-terminally, or vice versa.
  • peptide linker refers to a peptide comprising one or more amino acids.
  • a peptide linker comprises 1 to 44 amino acids, more particularly 2 to 20 amino acids.
  • Peptide linkers are known in the art or are described herein.
  • Suitable, non-immunogenic linker peptides are, for example, linkers that include G and/or S residues, (G4S)n, (SG4)n or G4(SG4)n peptide linkers, wherein “n” is generally a number between 1 and 10, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10.
  • the peptide is for example selected from the group consisting of GGGGS (SEQ ID NO: 790), GGGGSGGGGS (SEQ ID NO:791), SGGGGSGGGG (SEQ ID NO:792), GGGGSGGGGSGGGG (SEQ ID NO:793), GSGSGSGS (SEQ ID NO:794), GGSGSGSG (SEQ ID NO:795), GGSGSG (SEQ ID NO:796), GGSG (SEQ ID NO:797).
  • the fusion protein described above is capable of dual, e.g. simultaneous, engagement/binding to CD137 on the surface of effector cells and to PSMA displayed on the cell surface of tumor cells.
  • the dual, e.g. simultaneous binding leads to clustering of the CD137 receptor resulting in CD137 signalling. This leads to T cell activation.
  • dual, e.g. simultaneous binding leads to tumor antigen specific effector cell activation and results in tumor cell killing.
  • the fusion protein is capable of binding CD137 with an EC50 value that is similar or at least equivalent to the EC50 value by which the monovalent single heavy chain domain antibody binds to CD137. In some embodiments, the fusion protein binds CD137 with an EC50 value as shown in the examples.
  • the fusion protein described herein may be capable of co-stimulating T cell responses in a functional T cell activation essentially described as in the examples. In some embodiments, the fusion protein described herein may be able to induce IL-2 and/or IFN gamma secretion and T cell proliferation in a functional T cell activation.
  • the fusion polypeptide as described herein is, in some embodiments, also capable of local induction of IL-2 and/or IFN gamma secretion in the vicinity of the targeted tumor that is cells that are positive for the tumor antigen to which the fusion protein binds.
  • the fusion protein may be capable of producing a synergistic effect through dual targeting of the CD137 expressing cell and the tumor antigen expressing cell.
  • Dual, e.g. simultaneous targeting of CD137 and PSMA in the microenvironment of the tumor may enhance anti-tumor activity and reduce tumor growth. Moreover, by eliciting CD137 signalling locally, side effects may be reduced. CD137 signalling results in the recruitment of TRAF family members and activation of kinases. T cell mediated signalling protects CD8+ cells from activation induced death. Also provided is the use of the fusion protein as described herein for co-stimulating T cells.
  • a binding molecule as described herein binds to CD137 with a KD of at least about 10-6 M, alternatively at least about 10-7 M, alternatively at least about 10-8 M, alternatively at least about 10-9 M, alternatively at least about 10-10 M, alternatively at least about 10-11 M, alternatively at least about 10-12 M, or greater affinity as measured according to the methods shown in the examples.
  • a binding molecule as described herein binds to PSMA with a KD of at least about 10-6 M, alternatively at least about 10-7 M, alternatively at least about 10-8 M, alternatively at least about 10-9 M, alternatively at least about 10-10 M, alternatively at least about 10-11 M, alternatively at least about 10-12 M, or greater affinity as measured according to the methods shown in the examples. Binding can be measured as in the examples. In some embodiments, the binding molecules of the invention have IC50 and/or EC50 values as further described herein and as shown in the examples. Binding molecules described herein have shown excellent stability.
  • nucleic acid molecule encoding a fusion protein described herein.
  • the nucleic acid molecule comprises a nucleic acid encoding a single variable heavy chain domain antibody that binds to CD137 as specified herein and a nucleic acid encoding a single variable heavy chain domain antibody that binds to PSMA as specified herein.
  • a nucleic acid may comprise DNA or RNA and may be wholly or partially synthetic or recombinantly produced.
  • Reference to a nucleotide sequence as set out herein encompasses a DNA molecule with the specified sequence, and encompasses a RNA molecule with the specified sequence in which U is substituted for T, unless context requires otherwise.
  • the invention relates to a nucleic acid construct comprising at least one nucleic acid as defined above.
  • the construct may be in the form of a plasmid, vector, transcription or expression cassette.
  • the invention also relates to an isolated recombinant host cell comprising one or more nucleic acid construct as described above.
  • the host cell may be a bacterial, viral, plant, mammalian or other suitable host cell.
  • the cell is an E. coli cell.
  • the cell is a yeast cell.
  • the cell is a Chinese Hamster Ovary (CHO) cell.
  • a method of making the fusion protein as described herein comprises culturing the host cell under conditions suitable for expression of the polynucleotide encoding the fusion protein, and isolating the single domain antibody.
  • the binding molecules comprise a single variable heavy chain domain antibody that binds CD137 (CD137-binding subunit) and a moiety that binds PSMA (PSMA binding subunit).
  • the binding molecule can be a fusion protein wherein a single variable heavy chain domain antibody that binds CD137 is linked to another polypeptide that binds to PSMA.
  • the below provides examples of the CD137-binding subunit and the PSMA binding subunit.
  • single variable heavy chain domain antibodies that bind to CD137 and that may form one of the subunits of the binding molecule that bind to both, CD137 and PSMA, are described and can be used in the various embodiments of the invention.
  • CD137 is an important regulator of immune responses and therefore an important target in cancer therapy.
  • the T cell costimulatory receptor CD137 is induced on activated T cells and plays a variety of crucial roles: preventing activation-induced cell death (AICD), promoting cell cycle progression, enhancing cytotoxicity and the production of type 1 cytokines such as IL-2, IFN- ⁇ , and TNF- ⁇ , and increasing the memory CD8+ T cells.
  • AICD activation-induced cell death
  • type 1 cytokines such as IL-2, IFN- ⁇ , and TNF- ⁇
  • CD137 mediated anti-cancer effects are based on its ability to induce activation of cytotoxic T lymphocytes (CTL), and among others, high amounts of IFN- ⁇ .
  • CTL cytotoxic T lymphocytes
  • CD137/CD137L interactions are also considered positive regulators of CD8+ T cell responses against viruses such as influenza virus, lymphocytic choriomeningitis virus (LCMV), and herpes simplex virus (HSV).
  • viruses such as influenza virus, lymphocytic choriomeningitis virus (LCMV), and herpes simplex virus (HSV).
  • LCMV lymphocytic choriomeningitis virus
  • HSV herpes simplex virus
  • CD137 signalling requires clustering of the CD137 receptor. Such clustering is mediated by the interaction of the trimeric CD137 ligand with the CD137 receptor resulting in recruitment of signalling molecules such as the TRAF family of proteins. This in turn leads to kinase modulation and activation of the Nf-KB signalling pathway.
  • the NF-KB family of transcription factors has an essential role in inflammation and innate immunity. Furthermore, NF-KB is increasingly recognized as a crucial player in many steps of cancer initiation and progression.
  • Single domain antibodies described herein bind specifically to wild type human CD137 (UniProt Accession No. Q07011, GenBank Accession No. NM_001561).
  • the amino acid sequence (SEQ ID No. 786) and nucleotide sequences for wild type human CD137 are shown below (SEQ ID No. 787).
  • CD137 refers to human CD137.
  • CD137 is also known as “4-1BB”, “TNF receptor superfamily member 9”, “TNFRS9”, “induced by lymphocyte activation” and “ILA” these terms are used interchangeably, and include variants, isoforms of human CD137.
  • CD137 binding molecule/protein/polypeptide/agent/moiety refers to a molecule capable of specifically binding to the human CD137 antigen.
  • the binding reaction may be shown by standard methods, for example with reference to a negative control test using an antibody of unrelated specificity.
  • a multispecific binding agent described herein, “which binds” or is “capable of binding” an antigen of interest, e.g. human CD137, is one that binds the antigen with sufficient affinity such that the antibody is useful as a therapeutic agent in targeting a cell or tissue expressing the antigen CD137. Binding is to the extracellular domain of CD137.
  • Binding molecules of the invention bind specifically to human CD137. In other words, binding to the CD137 antigen is measurably different from a non-specific interaction. They do not cross react with mouse CD137.
  • the sdAb binds to human CD137 and also binds to monkey (e.g., cynomolgous) CD137.
  • the monovalent single domain antibody used in the multispecific molecule exhibits one or more of the following properties as a monovalent entity (i.e. when it is not provided in a multispecific format together with an entity that binds to PSMA):
  • (a) binds to human CD137 with a KD as measured in the examples; (b) binds to cells expressing CD137, but does not bind to cells that do not express CD137. This can be measured in a FMAT assay; (c) shows minimal cell internalisation. This can be measured as shown in the examples; (d) inhibits the interaction between CD137 ligand and CD137 expressed on the surface of cells. This can be measured in a FMAT assay. (e) does not activate CD137 signalling in T cells. This can be measured as shown in the examples; (f) does not stimulate IL-2 production from CD8+ cells.
  • the single variable heavy chain domain antibody comprises a CDR1, CDR2 or CDR3 as shown for one of the single domain antibodies as shown in Table 2 or a set of CDRs (i.e. CDR1, CDR2, CDR3) wherein said set is as shown for one of the single domain antibodies as shown in Table 2.
  • it comprises a CDR1, CDR2, CDR3 at least 40% or 75% homology thereto.
  • the single variable heavy chain domain antibody comprises a CDR1 comprising SEQ ID NO. 1 or a sequence with at least 40%, 75% or 80% homology thereto, a CDR2 comprising SEQ ID NO.
  • Sequence homology as above and as used generally herein can be at least 40%, 50%, 60%, 70%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% for example at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence homology.
  • the single variable heavy chain domain antibody comprises human framework regions.
  • the single variable heavy chain domain antibody according to the invention comprises or consists of a full length sequence as shown in Table 2 or a sequence with at least 50% homology thereto.
  • the single variable heavy chain domain antibody has a sequence selected from the sequences listed in Table 2, i.e. SEQ ID NO. 4, 8, 12, 16, 20 and so forth or a sequence with at least 50% homology thereto.
  • Sequence homology can be at least 50%, 60%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% for example at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence homology.
  • the single variable heavy chain domain antibody comprises a CDR1, 2, and 3 as shown for V H single domain antibodies 1.1 to 1.89 or 1.90 to 1.106 or comprises or consists of a full length sequence as shown for V H single domain antibodies 1.1 to 1.89 or 1.90 to 1.106 (i.e. SEQ ID NOs. 364, 368, 372, 376, 380, 384, 388, 392, 396, 400, 404, 408, 412, 416, 420 or 424).
  • the single variable heavy chain domain antibody comprises a CDR1, 2, and 3 as shown for V H single domain antibodies V H 1.107 to 1.114 as shown in table 2, e.g. SEQ ID No.
  • the single variable heavy chain domain antibody is selected from V H 1.107 to 1.114 as shown in table 2, i.e. V H 1.107, 1.108, 1.109, 1.110, 1.111, 1.112, 1.113 or 1.114, that is SEQ ID NOs. 852, 856, 860, 864, 868, 872, 876 or 880 or a sequence with at least 75%, 80% or 90% homology thereto.
  • the single variable heavy chain domain antibody is V H 1.113 or a sequence with at least 75%, 80% or 90% homology thereto.
  • the single variable heavy chain domain antibody comprises a CDR1, CDR2 or CDR3 as shown for one of the single domain antibodies as shown in Table 3 or comprising a CDR1, CDR2, CDR3 at least 75% homology thereto.
  • the single variable heavy chain domain antibody comprises a CDR1 comprising SEQ ID NO. 425 or a sequence with at least 80% homology thereto, a CDR2 comprising SEQ ID NO. 426 or a sequence with at least 75% homology thereto and a CDR3 comprising SEQ ID NO. 427 or a sequence with at least 75% homology, or a CDR1 comprising SEQ ID NO. 429 or a sequence with at least 75% homology thereto, a CDR2 comprising SEQ ID NO. 430 or a sequence with at least 75% homology thereto and a CDR3 comprising SEQ ID NO. 431 and so forth.
  • Sequence homology can be at least 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% for example at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence homology.
  • the single variable heavy chain domain antibody comprises human framework regions.
  • the single variable heavy chain domain antibody comprising or consisting of a full length sequence as shown in Table 3 or a sequence with at least 50% homology thereto.
  • the single variable heavy chain domain antibody has a sequence selected from those shown in Table 3, e.g. SEQ ID NO. 428, 432, 436, 440 and so forth or a sequence with at least 50% homology thereto.
  • Sequence homology can be at least 50%, 60%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% for example at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence homology.
  • the single variable heavy chain domain antibody comprises CDR1, 2, and 3 as shown for V H single domain antibodies 2.41 to 2.51 or comprises or consists of a full length sequence as shown for V H single domain antibodies 2.41 to 2.51 (i.e. SEQ ID NOs. 588, 592, 596, 600, 604, 608, 612, 616, or 620).
  • the binding molecule may comprise one or more single domain antibodies that bind to CD137, for example one or two single domain antibodies as described above in Tables 2 and 3.
  • V H single domain antibody that is a variant of any of the above single V H domain antibodies shown in Table 2 or Table 3 having one or more amino acid substitutions, deletions, insertions or other modifications, and which retains a biological function of the single domain antibody.
  • variant V H single domain antibodies can be sequence engineered. Modifications are described elsewhere herein.
  • a variant of V HS 1.07 to 1.113 for example V H 1.113 which has 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 substitutions.
  • the variant comprises one or more the following substitutions with reference to SEQ ID NO. 4 (V H 1.1) or combinations thereof:
  • the variant comprises one or more the following substitutions with reference to SEQ ID NO. 4 (V H 1.1) or combinations thereof:
  • the variant comprises one or more the following substitutions with reference to SEQ ID NO. 312 (V H 1.78) or combinations thereof:
  • the variant comprises one or more the following substitutions with reference to SEQ ID NO. 428 (V H 2.1) or combinations thereof:
  • the variant comprises one or more the following substitutions with reference to SEQ ID NO. 624 (V H 2.50) or combinations thereof:
  • nucleic acid may include DNA and/or RNA.
  • the present invention provides a nucleic acid that codes for a CDR, for example CDR3, a set of two or three CDRs or a V H single domain antibody of the invention as shown above.
  • the nucleic acid sequence has at least 50% sequence homology to one of the sequences selected above. In one embodiment, said sequence homology is at least 50%, 60%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99%. In one embodiment, the nucleic acid is selected from one SEQ ID Nos. 629, 706, 881, 882, 883, 884, 885, 886, 887 or 735.
  • moieties for example, single variable heavy chain domain antibodies, that bind to PSMA and that may form one of the subunits of the binding molecules that bind to both, CD137 and PSMA, are described and can be used in the various embodiments of the invention.
  • the PSMA binding molecules bind to wild type human PSMA (UniProt Accession NO. Q04609).
  • the sequence for the monomer is shown below (SEQ ID No. 842).
  • the PSMA binding molecules of the invention bind to wild type human PSMA and/or cyno PSMA.
  • PSMA binding molecule PSMA binding protein
  • anti-PSMA single domain antibody or “anti-PSMA antibody” as used herein all refer to a molecule capable of binding to the human PSMA antigen.
  • PSMA binding molecule includes a PSMA binding protein.
  • the binding reaction may be shown by standard methods (qualitative assays) including, for example, a binding assay, competition assay or a bioassay for determining the inhibition of PSMA binding to its receptor or any kind of binding assays, with reference to a negative control test in which an antibody of unrelated specificity.
  • a binding molecule of the invention including a single domain antibody and multivalent or multispecific binding agent described herein, “which binds” or is “capable of binding” an antigen of interest, e.g. PSMA, is one that binds, i.e. targets, the PSMA antigen with sufficient affinity such that it is useful in therapy in targeting a cell or tissue expressing the antigen.
  • an antigen of interest e.g. PSMA
  • the single variable heavy chain domain antibody comprises a CDR1 comprising SEQ ID NO. 812 or a sequence with at least 75% homology thereto, a CDR2 comprising SEQ ID NO. 813 or a sequence with at least 75% homology thereto and a CDR3 comprising SEQ ID NO. 814 or a sequence with at least 75% homology thereto.
  • the single variable heavy chain domain antibody comprises a CDR1 comprising SEQ ID NO. 837 or a sequence with at least 75% homology thereto, a CDR2 comprising SEQ ID NO. 838 or a sequence with at least 75% homology thereto and a CDR3 comprising SEQ ID NO. 839 or a sequence with at least 75% homology thereto.
  • Sequence homology can be 75, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% for example at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence homology.
  • the single variable heavy chain domain antibody comprises human framework regions. In one embodiment, the single variable heavy chain domain antibody is selected from single variable heavy chain domain antibody as shown in table 6 or 7, or from a sequence with at least 80% homology thereto.
  • the PSMA binding entity can also be selected form a part thereof, such as a CDR3.
  • the single variable heavy chain domain antibody comprises a sequence selected from SEQ ID NOs. 815, 816, 817, 818, 819, 820, 821, 822, 823, 824, 825, or 840.
  • the sequence is SEQ ID NO. 822 or from a sequence with at least 50%, 60%, 70% or 75% homology thereto.
  • Sequence homology can be 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% for example at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence homology.
  • the single variable heavy chain domain antibody comprises a sequence selected from SEQ ID NOs. 815, 816, 817, 818, 819, 820, 821, 822, 823, 824, 825, or 840 or a variant thereof, e.g. a variant with 1 to 10 or 1 to 20 substitutions.
  • the variant comprises substitutions. Typical modifications are explained elsewhere herein.
  • the binding molecule may comprise one or more single domain antibodies that bind to PSMA, for example one or two single domain antibodies as described above.
  • the binding molecule may comprise one or more single domain antibodies that bind to PSMA, for example one or two single domain antibodies as described above. In one embodiment the binding molecule comprises two single domain antibodies that bind to PSMA wherein each binds to a different epitope of PSMA hus providing a biparatopic PSMA binder.
  • any combination of the aforesaid single variable heavy chain domain antibodies that bind to CD137 and PSMA respectively can be used in a binding agent for dual engagement of CD137 and PSMA expressing cells.
  • any of the single variable heavy chain domain antibodies disclosed above, for example as listed in any of Tables 2 or 3 can be combined in a fusion protein with any of the single variable heavy chain domain antibodies as listed in any of Tables 6a, 6b or 7.
  • the fusion protein comprises a single domain antibody having a sequence selected from SEQ ID Nos 4, 8, 12, 16, 20, 24, 28, 48, 52, 56, 60, 64, 68, 72, 76, 80, 84, 88, 92, 96, 100, 104, 108, 112, 116, 120, 124, 128, 132, 136, 140, 144, 148, 152, 156, 160, 164, 168, 172, 176, 180, 184, 188, 192, 196, 200, 204, 208, 212, 216, 220, 224, 228, 232, 236, 240, 244, 248, 252, 256, 260, 264, 268, 272, 276, 280, 284, 288, 292, 296, 300, 304, 308, 312, 316, 320, 324, 328, 332, 336, 340, 344, 448, 352, 356, 360, 364, 368, 372, 376, 380, 384, 388
  • the fusion protein comprises a single domain antibody having a sequence selected from SEQ ID Nos 428, 432, 436, 440, 444, 448, 452, 456, 460, 464, 468, 472, 476, 480, 484, 488, 492, 496, 500, 508, 512, 516, 520, 524, 528, 532, 536, 540, 544, 548, 552, 556, 560, 564, 568, 572, 576, 580, 584, 588, 592, 596, 600, 604, 608, 612, 616, 620, 624 or 628 or a sequence with at least 75% homology thereto and a single domain antibody having a sequence selected from SEQ ID Nos 815, 816, 817, 818, 819, 820, 821, 822, 823, 824, 825 or 840 or a sequence with at least 75% homology thereto.
  • a single domain antibody 1.1 or 2.1 having SEQ ID No. 4 or 428 or a single domain antibody having SEQ ID No. 312, 852, 856, 860, 864, 868, 872, 876, 880 or 428 is linked to a single domain antibody 3.1, 3.8 or 4.1 having SEQ ID No. 815, 822 or 840 respectively.
  • a single domain 1.113 having SEQ ID No. 876 is linked to a single domain antibody 3.1, 3.8 or 4.1 having SEQ ID No. 815, 822 or 840 respectively.
  • a single domain 1.113 having SEQ ID No. 876 is linked to a single domain antibody 4.1 having SEQ ID No. 840.
  • the fusion protein comprises or consists of single domain antibody 1.113 linked to single domain antibody 4.1 or 3.8 and single domain antibody as shown in SEQ ID No. 901.
  • the order of the single domain antibodies can vary, for example the CD137 binding single domain antibody can located be 3′ or 5′ of the PSMA binding molecule.
  • the single domain antibody as shown in SEQ ID No. 901 is preferably located at the C terminal end.
  • the two single domain antibodies can be linked in the fusion protein with a peptide linker, such as a G4S linker as described herein.
  • a peptide linker such as a G4S linker as described herein.
  • the invention relates to one of the fusion proteins and nucleic acids encoding such fusion proteins as listed in table 8 below (i.e. selected from one of the protein and (i.e. selected from one of the protein and nucleic acid constructs of SEQ ID NO. 806-811) or fusion proteins and nucleic acids encoding such fusion proteins as listed in table 10 in the examples (i.e. selected from one of the protein and nucleic acid constructs of SEQ ID NO. 889-900).
  • the protein comprises the single domain antibodies defined as 4.1 or 1.113 as described herein linked with a (G4S)n peptide linker wherein n is as defined herein, e.g. 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 (i.e. 4.1-(G4S)n-1.113).
  • the protein is selected from SEQ ID Nos. 844, 846, 890 or 894.
  • the protein comprises or consists of SEQ ID NO. 890 optionally including a HSA binding sdAb.
  • the protein is encoded by the nucleic acid sequence of SEQ ID NO. 891.
  • a binding agent described above comprises further binding molecules.
  • the binding agent can, for example, be trispecific or tetraspecific.
  • the binding molecule comprises a first V H single domain antibody that binds to CD137 (V H (A)) and a second moiety, for example a V H single domain antibody, that binds to PSMA (V H (B)). It further comprises a third, fourth, fifth etc moiety, for example a V H single domain antibody (i.e. V H (C), V H (D), V H (E)) that binds to another antigen.
  • V H (C), V H (D), V H (E) binds to another antigen.
  • the binding molecule has the following formula (wherein V H stands for a single domain antibody as defined herein, that is the single V H domain that does not comprise other parts of a full antibody and retains binding to the antigen): V H (A)-L-V H (B)-L-V H (X)n wherein X denotes a V H binding to a target other than the target V H (A) and V H (B) bind to and wherein X is 1 to 10, for example, 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10.
  • L denotes a linker, for example a peptide linker.
  • a moiety that binds to PSMA or another target can be selected from an antibody or fragment thereof or other polypeptide.
  • the further moiety may serve to prolong the half-life of the binding molecule.
  • the further moiety may comprise a protein, for example a peptide, antibody, or part thereof, such as a V H or CDR, that binds a serum albumin, e.g., human serum albumin (HSA) or mouse serum albumin (MSA).
  • the further moiety may comprise a V H domain that binds serum albumin, e.g., human serum albumin (HSA) or mouse serum albumin (MSA).
  • the V H domain that binds HSA is SEQ ID NO. 901 or a sequence with at least 90% sequence homology thereto.
  • the further moiety may comprise a serum albumin, e.g. a HSA or a variant thereof such as HSA C34S.
  • a binding molecule as described herein comprising a V H domain and an Fc domain, e.g., wherein the V H domain is fused to an Fc domain.
  • half-life refers to the time taken for the serum concentration of the amino acid sequence, compound or polypeptide to be reduced by 50%, in vivo, for example due to degradation of the sequence or compound and/or clearance or sequestration of the sequence or compound by natural mechanisms.
  • Half-life may be increased by at least 1.5 times, preferably at least 2 times, such as at least 5 times, for example at least 10 times or more than 20 times, greater than the half-life of the corresponding V H single domain antibodies of the invention.
  • increased half-life may be more than 1 hours, preferably more than 2 hours, more preferably more than 6 hours, such as more than 12 hours, or even more than 24, 48 or 72 hours, compared to the corresponding V H single domain antibodies or fusion protein of the invention.
  • the in vivo half-life of an amino acid sequence, compound or polypeptide of the invention can be determined in any manner known per se, such as by pharmacokinetic analysis. Suitable techniques will be clear to the person skilled in the art. Half life can for example be expressed using parameters such as the t1/2-alpha t1/2-beta and the area under the curve (AUC).
  • the binding agents are labelled with a detectable or functional label.
  • a label can be any molecule that produces or can be induced to produce a signal, including but not limited to fluorophores, fluorescers, radiolabels, enzymes, chemiluminescers, a nuclear magnetic resonance active label or photosensitizers.
  • the binding may be detected and/or measured by detecting fluorescence or luminescence, radioactivity, enzyme activity or light absorbance.
  • binding agents are coupled to at least one therapeutic moiety, such as a drug, an enzyme or a toxin.
  • the therapeutic moiety is a toxin, for example a cytotoxic radionuclide, chemical toxin or protein toxin.
  • the binding agents of the invention are modified to increase half-life, for example by a chemical modification, especially by PEGylation, or by incorporation in a liposome or using a serum albumin protein. Increased half life can also be conferred by conjugating the molecule to a n antibody fragment, for example a V H domain that increases half life.
  • linker for example a polypeptide linker.
  • a single domain antibody described herein for use in the multispecifc molecule can be obtained from a transgenic rodent that expresses heavy chain only antibodies upon stimulation with a CD137 or PSMA antigen respectively.
  • the transgenic rodent for example a mouse, preferably has a reduced capacity to express endogenous antibody genes.
  • the rodent has a reduced capacity to express endogenous light and/or heavy chain antibody genes.
  • the rodent may therefore comprise modifications to disrupt expression of endogenous kappa and lambda light and/or heavy chain antibody genes so that no functional light and/or heavy chains are produced, for example as further explained below.
  • a method for producing a human heavy chain only antibody capable of binding the target antigen comprises
  • Further steps can include isolating a V H domain from said heavy chain only antibody, for example by generating a library of sequences comprising V H domain sequences from said rodent, e.g. mouse, and isolating sequences comprising V H domain sequences from said libraries.
  • a method for producing a single V H domain antibody capable of binding to the target antigen comprises
  • Further steps may include identifying a single V H domain antibody or heavy chain only antibody that binds to the target antigen, for example by using functional assays as shown in the examples.
  • Methods for preparing or generating the polypeptides, nucleic acids, host cells, products and compositions described herein using in vitro expression libraries can comprise the steps of:
  • the set, collection or library of amino acid sequences may be displayed on a phage, phagemid, ribosome or suitable micro-organism (such as yeast), such as to facilitate screening.
  • suitable methods, techniques and host organisms for displaying and screening (a set, collection or library of) amino acid sequences will be clear to the person skilled in the art (see for example Phage Display of Peptides and Proteins: A Laboratory Manual, Academic Press; 1st edition (Oct. 28, 1996) Brian K. Kay, Jill Winter, John McCafferty).
  • V H domain(s) are generated by isolating a cell or tissue expressing an antigen-specific, heavy chain-only antibody, cloning the sequence encoding the V H domain(s) from mRNA derived from the isolated cell or tissue and displaying the encoded protein using a library.
  • the V H domain(s) can be expressed in bacterial, yeast or other expression systems.
  • the term rodent may relate to a mouse or a rat.
  • the rodent is a mouse.
  • the mouse may comprise a non-functional endogenous lambda light chain locus.
  • the mouse does not make a functional endogenous lambda light chain.
  • the lambda light chain locus is deleted in part or completely or rendered non-functional through insertion, inversion, a recombination event, gene editing or gene silencing.
  • at least the constant region genes C1, C2 and C3 may be deleted or rendered non-functional through insertion or other modification as described above.
  • the locus is functionally silenced so that the mouse does not make a functional lambda light chain.
  • the mouse may comprise a non-functional endogenous kappa light chain locus.
  • the mouse does not make a functional endogenous kappa light chain.
  • the kappa light chain locus is deleted in part or completely or rendered non-functional through insertion, inversion, a recombination event, gene editing or gene silencing.
  • the locus is functionally silenced so that the mouse does not make a functional kappa light chain.
  • the mouse having functionally-silenced endogenous lambda and kappa L-chain loci may, for example, be made as disclosed in WO 2003/000737, which is hereby incorporated by reference in its entirety.
  • the mouse may comprise a non-functional endogenous heavy chain locus.
  • the heavy chain locus is deleted in part or completely or rendered non-functional through insertion, inversion, a recombination event, gene editing or gene silencing.
  • the locus is functionally silenced so that the mouse does not make a functional heavy chain.
  • all 8 endogenous heavy chain constant region immunoglobulin genes are absent in the mouse, or partially absent to the extent that they are non-functional, or genes ⁇ , ⁇ 3, ⁇ 1, ⁇ 2a, ⁇ 2b and are absent and the flanking genes ⁇ and ⁇ are partially absent to the extent that they are rendered non-functional, or genes ⁇ , ⁇ , ⁇ 3, ⁇ 1, ⁇ 2a, ⁇ 2b and ⁇ are absent and ⁇ is partially absent to the extent that it is rendered non-functional, or ⁇ , ⁇ 3, ⁇ 1, ⁇ 2a, ⁇ 2b, ⁇ and ⁇ are absent and ⁇ is partially absent to the extent that it is rendered non-functional, or ⁇ , ⁇ 3, ⁇ 1, ⁇ 2a, ⁇ 2b, ⁇ and ⁇ are absent and ⁇ is partially absent to the extent that it is rendered non-functional.
  • deletion in part is meant that the endogenous locus gene sequence has been deleted or disrupted, for example by an insertion, to the extent that no functional endogenous gene product is encoded by the locus, i.e., that no functional product is expressed from the locus.
  • the locus is functionally silenced.
  • the mouse comprises a non-functional endogenous heavy chain locus, a non-functional endogenous lambda light chain locus and a non-functional endogenous kappa light chain locus.
  • the mouse therefore does not produce any functional endogenous light or heavy chains.
  • the mouse is a triple knockout (TKO) mouse.
  • the transgenic mouse may comprise a vector, for example a Yeast Artificial Chromosome (YAC) for expressing a heterologous, preferably a human, heavy chain locus.
  • YACs are vectors that can be employed for the cloning of very large DNA inserts in yeast.
  • ARS autonomously replicating sequence
  • CEN centromere
  • TEL telomere
  • YACs The construction and use of YACs is well known in the art (e.g., Bruschi, C. V. and Gjuracic, K. Yeast Artificial Chromosomes, Encyclopedia of Life Sciences, 2002 Macmillan Publishers Ltd, Nature Publishing Group).
  • the YAC may comprise a plethora of unrearranaged human V H , D and J genes in combination with mouse immunoglobulin constant region genes lacking C H 1 domains, mouse enhancer and regulatory regions.
  • the human V H , D and J genes are human V H , D and J loci and they are unrearranged genes that are fully human.
  • Transgenic mice can be created according to standard techniques as illustrated in the examples.
  • the two most characterised routes for creating transgenic mice are via pronuclear microinjection of genetic material into freshly fertilised oocytes or via the introduction of stably transfected embryonic stem cells into morula or blastocyst stage embryos. Regardless of how the genetic material is introduced, the manipulated embryos are transferred to pseudo-pregnant female recipients where pregnancy continues and candidate transgenic pups are born.
  • the main differences between these broad methods are that ES clones can be screened extensively before their use to create a transgenic animal.
  • pronuclear microinjection relies on the genetic material integrating to the host genome after its introduction and, generally speaking, the successful incorporation of the transgene cannot be confirmed until after pups are born.
  • Transgenic animals can be generated by multiple means including random integration of the construct into the genome, site-specific integration, or homologous recombination.
  • tools and techniques that can be used to both drive and select for transgene integration and subsequent modification including the use of drug resistance markers (positive selection), recombinases, recombination-mediated cassette exchange, negative selection techniques, and nucleases to improve the efficiency of recombination. Most of these methods are commonly used in the modification of ES cells. However, some of the techniques may have utility for enhancing transgenesis mediated via pronuclear injection.
  • the endogenous mouse immunoglobulin expression is silenced to permit sole use of the introduced transgene for the expression of the heavy-chain only repertoire that can be exploited for drug discovery.
  • Genetically-manipulated mice for example TKO mice that are silenced for all endogenous immunoglobulin loci (mouse heavy chain, mouse kappa chain and mouse lambda chain) can be used as described above.
  • the transfer of any introduced transgene to this TKO background can be achieved via breeding, either conventional or with the inclusion of an IVF step to give efficient scaling of the process.
  • the oocytes may be derived from TKO donors.
  • ES cells from TKO embryos can be derived for use in transgenesis.
  • TKO/Tg Triple knock-out mice into which transgenes have been introduced to express immunoglobulin loci are referred to herein as TKO/Tg.
  • the mouse is as described in WO2016/062990.
  • Fusion proteins as described herein can be generated by linking a nucleic acid encoding a single variable heavy chain domain antibody that binds to CD137 to a nucleic acid encoding a single variable heavy chain domain antibody that binds to PSMA, for example using a nucleic acid sequence that encodes a peptide linker. Such fusion nucleic acid molecules are then expressed in suitable host cells.
  • a pharmaceutical composition comprising a binding molecule as described herein and optionally a pharmaceutically acceptable carrier.
  • a binding molecule as described herein or the pharmaceutical composition of the invention can be administered by any convenient route, including but not limited to oral, topical, parenteral, sublingual, rectal, vaginal, ocular, intranasal, pulmonary, intradermal, intravitreal, intramuscular, intraperitoneal, intravenous, subcutaneous, intracerebral, transdermal, transmucosal, by inhalation, or topical, particularly to the ears, nose, eyes, or skin or by inhalation.
  • Parenteral administration includes, for example, intravenous, intramuscular, intraarterial, intraperitoneal, intranasal, rectal, intravesical, intradermal, topical or subcutaneous administration.
  • the compositions are administered parenterally.
  • the pharmaceutically acceptable carrier or vehicle can be particulate, so that the compositions are, for example, in tablet or powder form.
  • carrier refers to a diluent, adjuvant or excipient, with which a drug antibody of the present invention is administered.
  • Such pharmaceutical carriers can be liquids, such as water and oils, including those of petroleum, animal, vegetable or synthetic origin, such as peanut oil, soybean oil, mineral oil, sesame oil and the like.
  • the carriers can be saline, gum acacia, gelatin, starch paste, talc, keratin, colloidal silica, urea, and the like.
  • auxiliary, stabilizing, thickening, lubricating and coloring agents can be used.
  • the single domain antibody of the present invention or compositions and pharmaceutically acceptable carriers are sterile.
  • Water is a preferred carrier when the drug antibody of the present invention are administered intravenously.
  • Saline solutions and aqueous dextrose and glycerol solutions can also be employed as liquid carriers, particularly for injectable solutions.
  • Suitable pharmaceutical carriers also include excipients such as starch, glucose, lactose, sucrose, gelatin, malt, rice, flour, chalk, silica gel, sodium stearate, glycerol monostearate, talc, sodium chloride, dried skim milk, glycerol, propylene, glycol, water, ethanol and the like.
  • the present compositions if desired, can also contain minor amounts of wetting or emulsifying agents, or pH buffering agents.
  • the pharmaceutical composition of the invention can be in the form of a liquid, e.g., a solution, emulsion or suspension.
  • the liquid can be useful for delivery by injection, infusion (e.g., IV infusion) or sub-cutaneously.
  • composition When intended for oral administration, the composition is preferably in solid or liquid form, where semi-solid, semi-liquid, suspension and gel forms are included within the forms considered herein as either solid or liquid.
  • the composition can be formulated into a powder, granule, compressed tablet, pill, capsule, chewing gum, wafer or the like form.
  • a solid composition typically contains one or more inert diluents.
  • binders such as carboxymethylcellulose, ethyl cellulose, microcrystalline cellulose, or gelatin; excipients such as starch, lactose or dextrins, disintegrating agents such as alginic acid, sodium alginate, corn starch and the like; lubricants such as magnesium stearate; glidants such as colloidal silicon dioxide; sweetening agents such as sucrose or saccharin; a flavoring agent such as peppermint, methyl salicylate or orange flavoring; and a coloring agent.
  • a liquid carrier such as polyethylene glycol
  • the composition can be in the form of a liquid, e. g. an elixir, syrup, solution, emulsion or suspension.
  • the liquid can be useful for oral administration or for delivery by injection.
  • a composition can comprise one or more of a sweetening agent, preservatives, dye/colorant and flavor enhancer.
  • a surfactant, preservative, wetting agent, dispersing agent, suspending agent, buffer, stabilizer and isotonic agent can also be included.
  • compositions can take the form of one or more dosage units.
  • composition can be desirable to administer the composition locally to the area in need of treatment, or by intravenous injection or infusion.
  • the amount of the therapeutic that is effective/active in the treatment of a particular disorder or condition will depend on the nature of the disorder or condition, and can be determined by standard clinical techniques. In addition, in vitro or in vivo assays can optionally be employed to help identify optimal dosage ranges.
  • the precise dose to be employed in the compositions will also depend on the route of administration, and the seriousness of the disease or disorder, and should be decided according to the judgment of the practitioner and each patient's circumstances. Factors like age, body weight, sex, diet, time of administration, rate of excretion, condition of the host, drug combinations, reaction sensitivities and severity of the disease shall be taken into account.
  • the amount is at least about 0.01% of a single domain antibody of the present invention by weight of the composition.
  • this amount can be varied to range from about 0.1% to about 80% by weight of the composition.
  • Preferred oral compositions can comprise from about 4% to about 50% of the single domain antibody of the present invention by weight of the composition.
  • compositions of the present invention are prepared so that a parenteral dosage unit contains from about 0.01% to about 2% by weight of the single domain antibody of the present invention.
  • the invention also relates to a device, such as a pre-filled syringe which comprises a binding molecule of the invention.
  • the composition can comprise from about typically about 0.1 mg/kg to about 250 mg/kg of the subject's body weight, preferably, between about 0.1 mg/kg and about 20 mg/kg of the subject's body weight, and more preferably about 1 mg/kg to about 10 mg/kg of the subject's body weight.
  • the composition is administered at a dose of about 1 to 30 mg/kg, e.g., about 5 to 25 mg/kg, about 10 to 20 mg/kg, about 1 to 5 mg/kg, or about 3 mg/kg.
  • the dosing schedule can vary from e.g., once a week to once every 2, 3, or 4 weeks.
  • treat means inhibiting or relieving a disease or disorder.
  • treatment can include a postponement of development of the symptoms associated with a disease or disorder, and/or a reduction in the severity of such symptoms that will, or are expected, to develop with said disease.
  • the terms include ameliorating existing symptoms, preventing additional symptoms, and ameliorating or preventing the underlying causes of such symptoms.
  • the terms denote that a beneficial result is being conferred on at least some of the mammals, e.g., human patients, being treated. Many medical treatments are effective for some, but not all, patients that undergo the treatment.
  • a subject refers to an animal which is the object of treatment, observation, or experiment.
  • a subject includes, but is not limited to, a mammal, including, but not limited to, a human or a non-human mammal, such as a non-human primate, murine, bovine, equine, canine, ovine, or feline.
  • the term “effective amount” means an amount of the binding molecule as described herein, that when administered alone or in combination with an additional therapeutic agent to a cell, tissue, or subject, is effective to achieve the desired therapeutic or prophylactic effect under the conditions of administration.
  • the invention furthermore relates to a method for the prevention and/or treatment of cancer, in particular prostate cancer, comprising administering a binding molecule of the invention to a subject, said method comprising administering, to a subject in need thereof, a pharmaceutically active amount of a binding molecule and/or of a pharmaceutical composition of the invention.
  • the invention relates to a method for the prevention and/or treatment of cancer, in particular prostate cancer, said method comprising administering, to a subject in need thereof, a pharmaceutically active amount of a binding molecule or a pharmaceutical composition of the invention.
  • the invention also relates to a binding molecule of the invention for use in the treatment of a disease.
  • the invention also relates to a binding molecule of the invention for use in the treatment of cancer, in particular prostate cancer or a prostatic disorder.
  • Prostate cancer refers to all stages and all forms of cancer arising from the tissue of the prostate gland.
  • the invention also relates to the treatment of a disease characterized by aberrant expression of PSMA.
  • the invention relates to the use of a binding molecule of the invention in the treatment of disease. In another aspect, the invention relates to the use of a binding molecule of the invention in the manufacture of a medicament for the treatment of cancer, such as prostate cancer or a prostatic disorder.
  • the binding molecules of the invention are also useful for the treatment, prevention, or amelioration of a disease.
  • the disease is associated with PSMA positive cells.
  • the disease is cancer.
  • the cancer associated with a PSMA positive tumor.
  • the cancer to be treated is selected from a tumor in the neovasculature that expresses PSMA, for example selected from renal, bladder, testicular, neuroendocrine, colon, and breast cancer.
  • the cancer is locally advanced unresectable, metastatic, or recurrent cancer.
  • the cancer is selected from the following non limiting list: prostate cancer, lung cancer or glioblastoma.
  • the disease is a prostatic disorder which refers to any disease that afflicts the prostate gland in the male reproductive system. The prostate gland is dependent on the hormonal secretions of the testes.
  • the binding molecule of the invention may be administered as the sole active ingredient or in combination with one or more other therapeutic and/or cytotoxic moiety.
  • the binding molecule may be conjugated to a toxic moiety.
  • the single domain antibody is used in combination with surgery.
  • the molecules or pharmaceutical composition of the invention may be administered as the sole active ingredient or in combination with one or more other therapeutic agent.
  • a therapeutic agent is a compound or molecule which is useful in the treatment of a disease. Examples of therapeutic agents include antibodies, antibody fragments, drugs, toxins, nucleases, hormones, immunomodulators, pro-apoptotic agents, anti-angiogenic agents, boron compounds, photoactive agents or dyes and radioisotopes.
  • An antibody molecule includes a full antibody or fragment thereof (e.g., a Fab, F(ab′)2, Fv, a single chain Fv fragment (scFv) or a single domain antibody, for example a V H domain, antibody mimetic protein or a protein that mimics the natural ligand of CD137.
  • a full antibody or fragment thereof e.g., a Fab, F(ab′)2, Fv, a single chain Fv fragment (scFv) or a single domain antibody, for example a V H domain, antibody mimetic protein or a protein that mimics the natural ligand of CD137.
  • the anti-cancer therapy may include a therapeutic agent or radiation therapy and includes gene therapy, viral therapy, RNA therapy bone marrow transplantation, nanotherapy, targeted anti-cancer therapies or oncolytic drugs.
  • therapeutic agents include other checkpoint inhibitors, antineoplastic agents, immunogenic agents, attenuated cancerous cells, tumor antigens, antigen presenting cells such as dendritic cells pulsed with tumor-derived antigen or nucleic acids, immune stimulating cytokines (e.g., IL-2, IFNa2, GM-CSF), targeted small molecules and biological molecules (such as components of signal transduction pathways, e.g.
  • modulators of tyrosine kinases and inhibitors of receptor tyrosine kinases, and agents that bind to tumor-specific antigens including EGFR antagonists
  • an anti-inflammatory agent including a cytotoxic agent, a radiotoxic agent, or an immunosuppressive agent and cells transfected with a gene encoding an immune stimulating cytokine (e.g., GM-CSF), chemotherapy.
  • administration is in combination with surgery.
  • the binding molecule of the invention may be administered at the same time or at a different time as the other therapy, e.g., simultaneously, separately or sequentially.
  • a method of modulating an immune response in a subject comprising administering to the subject the binding molecule or pharmaceutical composition described herein such that the immune response in the subject is modulated.
  • the binding molecule enhances, stimulates or increases the immune response in the subject.
  • a method of inhibiting growth of tumor cells or promoting tumor regression in a subject comprising administering to a subject a therapeutically effective amount of a binding molecule or a pharmaceutical composition described herein.
  • a method for activating the downstream signalling pathway of CD137 comprising administering to a subject a binding molecule or a pharmaceutical composition described herein.
  • a method for inducing T lymphocyte activation and/or proliferation comprising administering to a subject a binding molecule or a pharmaceutical composition described herein.
  • a method for dual targeting of a CD137 expressing cell and a tumor antigen, i.e. PSMA, expressing cell comprising administering to a subject a binding molecule comprising a single variable heavy chain domain antibody that binds to CD137 or a pharmaceutical composition described herein.
  • a binding molecule comprising a single variable heavy chain domain antibody that binds to CD137 or a pharmaceutical composition described herein for dual targeting of a CD137 expressing cell and a tumor antigen, i.e. PSMA, expressing cell.
  • the binding molecule comprises a single variable heavy chain domain antibody that binds to CD137 described herein and a single variable heavy chain domain antibody that binds to PSMA described herein
  • an immunoconjugate comprising a binding molecule described herein conjugated to at least one therapeutic and/or diagnostic agent i.e. an imagining agent.
  • kits in another aspect, provides a kit for detecting prostate cancer for diagnosis, treatment, prognosis or monitoring comprising a binding molecule of the invention.
  • the kit may also comprise instructions for use.
  • the kits may include a labeled binding molecule of the invention as described above and one or more compounds for detecting the label.
  • a binding molecule of the invention packaged in lyophilized form, or packaged in an aqueous medium.
  • the kits may include reagent, (e.g. for reconstituting) and/or instructions for use and/or a device for administration.
  • the invention also relates to detection methods using the binding molecule of the invention.
  • the human-PSMA-binding molecules as disclosed herein can be used to detect PSMA (e.g., in a biological sample, such as serum or plasma), using a conventional immunoassay, such as an enzyme linked immunosorbent assays (ELISA), an radioimmunoassay (RIA) or tissue immunohistochemistry.
  • ELISA enzyme linked immunosorbent assays
  • RIA radioimmunoassay
  • tissue immunohistochemistry tissue immunohistochemistry.
  • the invention also relates to in vitro or in vivo methods for diagnosing or monitoring progression of a cancer, in particular prostate cancer.
  • In vitro methods comprise detecting the presence of a PSMA protein in a test sample and comparing this with control sample from a normal subject or with a standard value or standard value range for a normal subject.
  • the sample may be selected from blood, plasma, serum, semen, urine or a tissue biopsy.
  • the method may include: (a) contacting the sample (and optionally, a reference, e.g., a positive and/or negative control sample) with a PSMA binding molecule of the invention and (b) detecting either the binding molecule bound to PSMA or unbound binding molecule in the sample, to thereby detect PSMA in the biological sample.
  • the binding molecule can be directly or indirectly labeled with a detectable substance to facilitate detection of the bound or unbound antibody. Suitable detectable substances include various enzymes, prosthetic groups, fluorescent materials, luminescent materials and radioactive materials.
  • In vivo methods may comprise detecting the presence of PSMA in vivo, for example by imaging in a subject.
  • a PSMA binding molecule of the invention is labeled to detect binding.
  • a labelled molecule of the invention may thus be used as an imaging agent.
  • human PSMA can be assayed in biological fluids by a competition immunoassay utilizing PSMA standards labeled with a detectable substance and an unlabeled human PSMA binding molecule.
  • the biological sample, the labeled PSMA standards and the human PSMA binding molecule are combined and the amount of labeled PSMA standard bound to the unlabeled binding molecule is determined.
  • the amount of human PSMA in the biological sample is inversely proportional to the amount of labeled PSMA standard bound to the PSMA binding molecule.
  • human PSMA can also be assayed in biological fluids by a competition immunoassay utilizing PSMA standards labeled with a detectable substance and an unlabeled human PSMA binding molecule.
  • Binding molecules disclosed herein can be used to inhibit PSMA activity, e.g., in a cell culture containing PSMA, in human subjects or in other mammalian subjects having PSMA with which a binding molecule disclosed herein cross-reacts.
  • a method for inhibiting or increasing PSMA activity comprising contacting PSMA with a binding molecule disclosed herein such that PSMA activity is inhibited or increased.
  • a binding molecule disclosed herein can be added to the culture medium to inhibit PSMA activity in the culture.
  • the invention also relates to a method of ablating or killing a cell that expresses PSMA, e.g., a cancerous or non-cancerous prostatic cell.
  • Methods of the invention include contacting the cell, with PSMA binding molecule of the invention, in an amount sufficient to ablate or kill the cell.
  • the methods can be used on cells in culture, e.g., in vitro or ex vivo.
  • Humabody® V H specific for CD137 or PSMA were generated by immunisation of the Crescendo's proprietary transgenic mouse that has silenced murine immunoglobulin loci and a transgene containing human heavy chain antibody genes.
  • mice carrying a human heavy-chain antibody transgenic locus in germline configuration within a background that is silenced for endogenous heavy and light chain antibody expression were created as previously described (WO2004/076618, WO2003/000737, Ren et al., Genomics, 84, 686, 2004; Zou et al., J. Immunol., 170, 1354, 2003 and WO2016/062990).
  • transgenic mice were derived following pronuclear microinjection of freshly fertilised oocytes with a yeast artificial chromosome (YAC) comprising a plethora of human V H , D and J genes in combination with mouse immunoglobulin constant region genes lacking C H 1 domains, mouse enhancer and regulatory regions.
  • YAC yeast artificial chromosome
  • the YAC used comprised multiple human heavy chain V genes, multiple human heavy chain D and J genes, a murine C H 1 gene and a murine 3′ enhancer gene. It lacks the C H 1 exon.
  • V H Heavy chain variable domains
  • the PSMA-binding subunit may be generated as disclosed in WO2017/122017.
  • VH that bind CD137 Tg/TKO mice aged 8-12 weeks were immunised with human CD137-human Fc chimeric protein (Acro Biosystems cat no. 41B-H5258), human CD137-His tagged protein (R&D Systems, custom product), CHO cells over-expressing human CD137 (cell line produced in-house using standard methods) or a combination of recombinant protein and CHO human CD137 expressing cells. Serum was then collected from mice before and after immunisation and checked by ELISA for the presence of serum human CD137 reactive heavy chain antibodies in response to immunisation with CD137 antigen.
  • RNAlater® was collected into RNAlater® from several immunised mice. Total RNA was extracted from supernatants and
  • V H sequences were mined from the RNA samples using Superscript III RT-PCR high-fidelity kit (Invitrogen cat. no. 12574-035) according to the manufacturer's protocol. Preparation of library phage stocks and phage display selections were performed according to published methods (Antibody Engineering, edited by Benny Lo, chapter 8, p 161-176, 2004). In most cases, phage display combined with a panning approach was used to isolate binding V H domains. However, a variety of different selection methods are well described in the art, including soluble protein selections, cell based selections and selections performed under stress (e.g., heat).
  • stress e.g., heat
  • V H that bound to CHO cells expressing human CD137 did not bind to CHO parental cells and inhibited the interaction between human CD137 expressed on the surface of CHO cells and recombinant human CD137 Ligand protein were identified by single point screening of bacterial periplasmic extracts. Binding of His-tagged V H in the supernatants to CHO human CD137 cells and to CHO parent cells for determination of non CD137 specific binding was assessed using Fluorescence Microvolume Assay Technology (FMAT). In parallel to the binding assay, periplasmic extracts were tested for their ability to inhibit the interaction of human CD137 ligand protein with CHO human CD137 cells in an FMAT format.
  • FMAT Fluorescence Microvolume Assay Technology
  • V H Families of V H were identified that bound to the CHO human CD137 cells, did not bind CHO parental cells and that inhibited CD137 binding to CD137 Ligand.
  • Each individual V H clone as identified above was sequenced from the phagemid and grouped based on V H germline and CDR3 amino acid similarity. Representative clones were further characterised. Further clones were generated by sequence optimisation of clone Humabody® V H 1.1 and Humabody® V H 2.1 respectively to improve binding activity, revert sequence to germline or remove biophysical sequence liabilities such as isomerisation or deamidation sites. Purified V H were obtained by using standard procedures. Table 2 shows the sequences of Family 1 Vs and table 3 those of Family 2 V H s.
  • V H Purified Humabody® V H were tested for binding to human CD137 protein, rhesus CD137Fc recombinant protein, mouse CD137 protein, tumour necrosis factor receptor family members OX40 and GITR (Glucocorticoid-induced TNFR-related), CHO human CD137 cells, CHO parent cells and human T-cells.
  • VH bound to human and rhesus CD137 but not to mouse CD137 protein.
  • Binding of serially diluted V H to CHO human CD137 cells and CHO parent cells were performed using an FMAT assay format.
  • V H bound to CHO human CD137 expressing cells but did not bind to CHO parental cells. Binding of monovalent single domain antibodies to primary T cells was measured using flow cytometry.
  • Humabody® V H as described herein bound to pre-stimulated CD8+ cells.
  • the ability of purified Humabody V H to inhibit the binding of CD137 Ligand to CHO human CD137 cells was also measured in the FMAT ligand inhibition assay.
  • V H inhibited the binding of human CD137 Ligand to human CD137.
  • a functional assay was also carried out to assess the ability of monovalent V H that bind to CD137, to act as CD137 agonists was assessed in a reporter gene assay using Jurkat cells expressing CD137 and an NF-kB luciferase reporter gene. Their activity was compared to bivalent and trivalent molecules which have increased potential for avid interactions and to bispecific molecules consisting of CD137 V H linked to a V H that bound to the tumour antigen PSMA. In the bispecific molecule, CD137 agonism resulted from co-engagement of both CD137 and the cell expressed PSMA.
  • Multivalent constructs were generated by linking isolated Humabody® V H nucleic acid sequences using linkers of encoding glycine/serine rich sequences (G4S) x where x is the number of G4S repeats and ranges from 2 to 12 repeat units.
  • DNA sequences encoding each Humabody® were amplified by PCR. The products were assembled into larger fragments with the V H single domain antibody sequences flanked by the (G4S) x linkers and ligated into an expression vector by a restriction enzyme-based method. Plasmids were transformed into microbial expression systems as per standard molecular biology techniques. The presence of inserts were verified by standard colony PCR technique and sequence confirmed by Sanger sequencing using vector-specific and internal primers to ensure complete sequence coverage.
  • bispecific molecules For generation of bispecific molecules the first and second sequence were not the same with one sequence corresponding to a Humabody® V H specific for CD137 and the other to a Humabody® that binds the tumour associated antigen PSMA.
  • monovalent and bispecific binding agents were optionally linked to a half-life extending Humabody® nucleic acid sequence (MSA binder).
  • MSA binder half-life extending Humabody® nucleic acid sequence
  • Bispecific and trispecific constructs were expressed by microbial cell systems. For stability testing purified bispecific and trispecific V H were subjected to size exclusion chromatography. Example data for representative constructs is shown in table 9.
  • trispecific fusion proteins and nucleic acids encoding such fusion proteins are shown below.
  • bivalent molecules are also shown below (table 10).
  • Binding of His-tagged molecules to CHO human CD137, CHO parent, CHO human PSMA, DU145 PSMA and DU145 parent cells was assessed using Fluorescence Microvolume Assay Technology (FMAT). All reagents were prepared in FMAT assay buffer (pH 7.4) containing PBS, 0.1% Bovine Serum Albumin, 0.05% Sodium Azide. Serially diluted samples were transferred into 384 well black clear-bottomed assay plates (Costar cat. no. 3655) and incubated for a minimum of 2 hours at room temperature with 1.5 nM Anti-His (Millipore cat. no. 05-949), 3 nM Goat Anti-Mouse Alexa Fluor-488 (Jackson ImmunoResearch cat. no.
  • Example EC 50 values for binding are shown in table 11. Monovalent CD137 specific Humabody® V H , bispecific and trispecific molecules with a CD137 binding arm bound to CHO CD137 expressing cells. Monovalent PSMA specific Humabody® V H , bispecific and trispecific molecules with a PSMA binding arm bound to PSMA expressing cells.
  • PBMCs Peripheral blood mononuclear cells
  • CD8+ T cells purified using a negative selection isolation kit according to the manufacturer's protocol (Miltenyi Biotech cat no 130-042-401).
  • CD8+ T cells were stimulated PMA/lonomycin for 48-72 hours in RPMI media supplemented with 10% FBS, 2 mM Glutamine, 1 ⁇ Pen/Strep.
  • CD137-Fc tag protein Acro Biosystems cat no. 41B-H5258
  • PSMA-his R&D Systems cat no. 4234-ZN
  • Monovalent V H and bispecific molecules were serially diluted (typically 1:2 dilution series starting between 12-25 nM, at the highest concentration) in kinetics buffer (0.1% BSA, 0.02% Tween, 1 ⁇ PBS) and binding to the immobilised proteins was studied during the association and dissociation phases.
  • PSMA binding was measured using 180 seconds association and 600 seconds dissociation phases.
  • CD137 binding was measured 180 seconds association and 600 seconds dissociation phases.
  • Reference subtracted data were fitted to a 1:1 binding model using the ForteBio Octet Data Analysis software. Example kinetic and binding affinity data obtained are shown in Table 12.
  • the Biacore T200 instrument was used to study the interaction between VH with human and rhesus CD137-human IgG1 Fc tagged protein by surface plasmon resonance (SPR).
  • SPR surface plasmon resonance
  • Single cycle kinetics assays used to evaluate the kinetics and affinity of the interaction. Experiments were performed at 25° C. in HBS-EP+ assay buffer with a flow rate of 30 ⁇ l/minute.
  • a Protein G chip was used to capture the Fc tagged recombinant CD137 diluted to 2 ⁇ g/ml to one of the flow cells over 7 seconds.
  • a second flow cell without any captured CD137 was used as the reference cell.
  • a five point, three-fold dilution series of V H was made with a top concentration of 60 nM.
  • the binding kinetics were followed by flowing these over the chip surface.
  • the contact time for each of the binding steps was 180 seconds and the dissociation step was 1800 and 3600 seconds for rhesus and human CD137 respectively.
  • the sensors were regenerated with glycine pH 1.5 to remove the captured CD137.
  • the data was fitted to a 1:1 binding model after double reference subtraction using the Biacore T200 Evaluation software.
  • Average kinetic constants ( ⁇ Standard deviation) for Humabody® 1.113 for binding to human CD137Fc were ka 3.6E+06 ⁇ 1.6E+06 (1/Ms), Kd is 3.0E-04 ⁇ 1.1E-04 (1/s) and KD 8.5E-11 ⁇ 7.8E-12 (M) and for binding to rhesus CD137Fc were ka 1.1E+06 ⁇ 2.2E+05 (1/Ms), Kdis 2.8E-04 ⁇ 6.8E-06 (1/s) and KD 2.7E-10 ⁇ 5.2E-11.
  • Average kinetic constants ( ⁇ Standard deviation) for construct 4.1-6GS-1.113-6GS-V H that binds HSA-4GS for binding to human CD137Fc were ka 5.6E+05 (1/Ms), Kdis 1.7E-05 (1/s) and KD 3.1E-11 (M) and for binding to rhesus CD137Fc were ka 4.4E+05 (1/Ms), Kdis 5.2E-05 (1/s) and KD 1.2E-10 (M).
  • Humabody® 1.113 showed better binding to cyno compared to other molecules and also showed better developability characteristics (stability and expression) compared to other molecules.
  • FIG. 1 shows representative data demonstrating that bispecific molecules can simultaneously bind both human CD137 and human PSMA.
  • Human CD8+ T cells pre-stimulated for 48 hours with PMA/ionomycin were washed with FACS buffer (PBS supplemented with 10% human serum and 0.05% sodium azide) by centrifugation. Serially diluted bispecific and trispecific Humabody VH construct were added to the cells for 1 hour on ice.
  • Binding to cell lines was also measured using Fluorescence Microvolume Assay technology as per section 3.1. CD137huFc/anti humanFc-Alexa Fluor-488 was used for detection of binding to PSMA expressing cells.
  • Flow cytometry data shown is the average EC50 ( ⁇ SD) for a minimum of 6 different human T-cell donors (Table 13).
  • Construct 4.1-6GS-1.113-6GS-VH HSA-4GS bound CHO cynomolgus PSMA cells in the FMAT assay (Average EC 50 ⁇ SD (M) 5.7E-10 ⁇ 4.6E-11, 0.57 nM n 3).
  • Humabody® V HS were tested for their ability to inhibit the interaction of human CD137-ligand protein with CHO human CD137 cells in an FMAT format. Serially diluted V H in FMAT assay buffer were incubated for a minimum of 2 hours at room temperature with 0.2 nM 0.4 nM CD137L-huFc (Sino Biologicals cat no. 15693-101H), 3 nM Alexa Fluor® 488 Goat Anti-Human IgG, Fc gamma fragment specific goat anti human IgG (Jackson ImmunoResearch cat no. 109-545-098) and 2000 cells pre-stained with DRAQ5.
  • Total binding controls containing FMAT assay buffer and non-specific binding controls containing excess non-Fc tagged competitor were set up on each plate for data normalisation. Fluorescence signal was measured using the TTP Mirrorball and the FL2 median mean fluorescence intensity of gated used for the data normalisation. The data was expressed as a % of the total binding control (% control) after subtraction of the background signal determined from the non-specific binding control wells. V H in monovalent and bispecific formats inhibited CD137 binding to CD137 Ligand (Table 14).
  • bispecific molecules to act as CD137 agonists was assessed in a co-culture reporter gene assay using Jurkat cells expressing CD137 and an NF-kB luciferase reporter gene and cells expressing human PSMA. Assays were performed using high PSMA expressing cells (CHO PSMA) and low PSMA expressing cells (DU145 PSMA). PSMA expressing cells or parental cells (5000/well) were plated overnight into 384 well, white clear bottomed tissue culture treated plates. For CHO cells media was removed the next day and replace by assay media (RPMI 1640 supplemented with 10% FBS, 2 mM L-Glutamine, 1 ⁇ Pen/Strep).
  • FIG. 2 exemplifies the concentration and PSMA dependent stimulation of the NF-kB pathway by CD137-PSMA V H bispecific molecules.
  • the level of PSMA expression determines the maximum response with higher maximal levels obtained for the CHO PSMA high PSMA expressing cell line compared to the low expressing DU145 PSMA cells ( FIG. 2 b ).
  • Anti CD137 antibody stimulates NF-kB driven reporter gene activity in a PSMA independent manner ( FIG. 2 c ).
  • the experiments show that in the bispecific molecule, CD137 agonism resulted from co-engagement of both CD137 and the cell expressed PSMA.
  • Bispecific molecules were tested for their ability to induce IL-2 release from T cells in a co-culture assay.
  • DU145 PSMA or parental DU145 cells were resuspended in media (RPMI 1640 supplemented with 10% FBS, 2 mM L-Glutamine, 1 ⁇ Pen/Strep) and seeded at a density of 20000 per well onto 96 well flat bottom plates that had been pre-coated with 5 ug/ml anti CD3 antibody (e-Bioscience cat no. 14-0037-82). Cells were allowed to adhere overnight at 37° C., 5% CO 2 .
  • PBMCs Peripheral blood mononuclear cells
  • CD8+ T cells purified using a negative selection isolation kit according to the manufacturer's protocol (Miltenyi Biotech cat no 130-042-401).
  • Humabody® V H bispecifics and benchmark antibodies were prepared in media and added together with the T cells (100000 cells/well) to the assay plates.
  • Supernatants were harvested after a 48 hour incubation at 37° C., 5% CO 2 and IL-2 levels quantified using a human IL-2 assay kit according to the manufacturer's instructions (Cisbio Cat no. 641L2PEB).
  • PBMC from healthy donor were stimulated with 10 ng/ml SEB (Staphylococcal enterotoxin B) for 16 hours prior to treatment.
  • SEB Staphylococcal enterotoxin B
  • CHO cells or CHO cells expressing PSMA were plated into 96-well plates at 10,000 per well.
  • Humabody® constructs were added to a final concentration of 50 nM and a 4-fold dilution series.
  • SEB-stimulated PBMC were added at 75,000 per well in media with 1 ng/ml SEB. Plates were incubated at 37° C. 5% CO 2 for 3 days. Supernatants were harvested for cytokine measurement.
  • TNF-alpha was measured using Cisbio HTRF kit (62HTNFAPEG) according to manufacturer's instructions. TNF-alpha increased in a bispecific Humabody® dependant dose-response manner in the presence of cells expressing PSMA. There was no induction in the absence of PSMA (
  • mice Male NCG mice (NOD-Prkdcem26Cd52112rgem26Cd22/NjuCrl, Charles River) were injected sub-cutaneously in the right flank with 1 ⁇ 10 7 DU145 PSMA cells in 50% matrigel. On Day 8, hPBMCs (HemaCare BioResearch Products) were engrafted via tail vein. Non engrafted mice were used as control groups. Mice were then treated with Humabody® or control CD137 agonist antibody administered intraperitoneally and body weights, clinical observations, and tumour volumes recorded.
  • hPBMCs HemaCare BioResearch Products
  • the hFcRn/HSA humanized mouse provides more predictable “human-like” pharmacokinetic results than WT mice. This model is well suited for in vivo assessment of HSA-binding drugs' pharmacokinetic, distribution and toxicity.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicinal Preparation (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
US16/763,063 2017-11-13 2018-11-13 Molecules that bind to cd137 and psma Pending US20200362051A1 (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
GBGB1718734.5A GB201718734D0 (en) 2017-11-13 2017-11-13 Single domain antibodies that bind to CD137
GB1718734.5 2017-11-13
GB1718735.2 2017-11-13
GBGB1718735.2A GB201718735D0 (en) 2017-11-13 2017-11-13 Bispecific molecules that bind to CD137 and PSMA
GB1808589.4 2018-05-24
GBGB1808589.4A GB201808589D0 (en) 2018-05-24 2018-05-24 Single domain antibodies that bind CD137
PCT/GB2018/053280 WO2019092452A1 (en) 2017-11-13 2018-11-13 Molecules that bind to cd137 and psma

Publications (1)

Publication Number Publication Date
US20200362051A1 true US20200362051A1 (en) 2020-11-19

Family

ID=64457031

Family Applications (2)

Application Number Title Priority Date Filing Date
US16/763,063 Pending US20200362051A1 (en) 2017-11-13 2018-11-13 Molecules that bind to cd137 and psma
US16/763,059 Active 2041-02-03 US12077595B2 (en) 2017-11-13 2018-11-13 Single domain antibodies that bind to CD137

Family Applications After (1)

Application Number Title Priority Date Filing Date
US16/763,059 Active 2041-02-03 US12077595B2 (en) 2017-11-13 2018-11-13 Single domain antibodies that bind to CD137

Country Status (10)

Country Link
US (2) US20200362051A1 (hr)
EP (2) EP3710478A1 (hr)
JP (3) JP7312168B2 (hr)
KR (2) KR20200083574A (hr)
CN (3) CN111699197B (hr)
AU (2) AU2018363292A1 (hr)
CA (2) CA3082321A1 (hr)
IL (2) IL274370A (hr)
SG (2) SG11202003912RA (hr)
WO (2) WO2019092452A1 (hr)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113621065A (zh) * 2021-08-30 2021-11-09 武汉海沙百得生物技术有限公司 靶向4-1bb的全人源抗体及其制备方法和应用
US11236174B2 (en) 2016-01-12 2022-02-01 Crescendo Biologics Limited Therapeutic molecules
US11401342B2 (en) * 2017-07-10 2022-08-02 Crescendo Biologics Limited Therapeutic molecules binding PSMA
US11591398B2 (en) 2017-01-06 2023-02-28 Crescendo Biologics Limited Single domain antibodies to programmed cell death protein 1 (PD-1)
WO2023030258A1 (en) * 2021-08-31 2023-03-09 LaNova Medicines Limited Anti-4-1bb nanobodies
US11866510B2 (en) 2016-05-06 2024-01-09 Crescendo Biologics Limited Chimeric antigen receptor with single domain antibody
US11951172B2 (en) 2018-02-16 2024-04-09 Crescendo Biologics Limited Therapeutic molecules that bind to LAG3 and PD1
US12077595B2 (en) 2017-11-13 2024-09-03 Crescendo Biologics Limited Single domain antibodies that bind to CD137

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2020132572A (ja) * 2019-02-20 2020-08-31 旭化成株式会社 シラノール組成物、硬化物及び製造方法
SG11202111671QA (en) * 2019-05-15 2021-11-29 Crescendo Biologics Ltd Binding molecules
PE20211147A1 (es) * 2019-08-12 2021-06-28 I Mab Biopharma Us Ltd Anticuerpos anti-claudina 18.2 y anti-4-1bb biespecificos y su uso
US20220411523A1 (en) * 2019-11-13 2022-12-29 Hefei Hankemab Biotechnology Co., Ltd Molecule capable of binding to human 4-1bb and its application thereof
EP4061837A1 (en) * 2019-11-18 2022-09-28 Janssen Biotech, Inc. Anti-cd79 chimeric antigen receptors, car-t cells, and uses thereof
JP2023531042A (ja) * 2020-06-30 2023-07-20 諾納生物（蘇州）有限公司 ４－１ｂｂ結合タンパク質及びその用途
JP2023542257A (ja) 2020-09-16 2023-10-05 アムジェン インコーポレイテッド 癌の治療のために二重特異性ｔ細胞誘導分子の治療用量を投与する方法
JP2024522078A (ja) * 2021-05-21 2024-06-11 ベイジーン スウィッツァーランド ゲーエムベーハー 抗ｇｐｃ３及び抗ｃｄ１３７多重特異性抗体ならびにそれらの使用方法
JP2024521701A (ja) * 2021-05-21 2024-06-04 ベイジーン スウィッツァーランド ゲーエムベーハー 抗ｃｄ１３７抗体及びその使用方法
WO2024040195A1 (en) 2022-08-17 2024-02-22 Capstan Therapeutics, Inc. Conditioning for in vivo immune cell engineering
WO2024061364A1 (zh) * 2022-09-22 2024-03-28 成都盛世君联生物技术有限公司 一种抗4-1bb纳米抗体及其制备和应用
WO2024109678A1 (en) * 2022-11-21 2024-05-30 Beigene, Ltd. Anti-cd137 antibodies and methods of use

Family Cites Families (70)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0115256D0 (en) 2001-06-21 2001-08-15 Babraham Inst Mouse light chain locus
CA2464239C (en) 2001-10-23 2016-07-12 Psma Development Company, L.L.C. Psma antibodies and protein multimers
GB2398784B (en) 2003-02-26 2005-07-27 Babraham Inst Removal and modification of the immunoglobulin constant region gene cluster of a non-human mammal
GB0400440D0 (en) 2004-01-09 2004-02-11 Isis Innovation Receptor modulators
SG188175A1 (en) * 2004-06-03 2013-03-28 Novimmune Sa Anti-cd3 antibodies and methods of use thereof
GB0521621D0 (en) * 2005-10-24 2005-11-30 Domantis Ltd Tumor necrosis factor receptor 1 antagonists for treating respiratory diseases
MX2007009878A (es) 2005-02-18 2007-10-03 Medarex Inc Anticuerpos monoclonales para antigeno de membrana especifica para prostata (amep).
EP1919504B1 (en) 2005-08-03 2013-10-16 iBio, Inc. Antibody to bacillus anthracis protective antigen
US8168757B2 (en) 2008-03-12 2012-05-01 Merck Sharp & Dohme Corp. PD-1 binding proteins
CA2718705A1 (en) 2008-03-17 2009-09-24 Allergan, Inc. S1p3 receptor inhibitors for treating inflammation
US20100122358A1 (en) 2008-06-06 2010-05-13 Crescendo Biologics Limited H-Chain-only antibodies
JP2011528804A (ja) * 2008-07-18 2011-11-24 オラジェニックス，インコーポレイテッド 結腸直腸癌の検出及び治療のための組成物
US8552154B2 (en) 2008-09-26 2013-10-08 Emory University Anti-PD-L1 antibodies and uses therefor
US9120855B2 (en) * 2010-02-10 2015-09-01 Novartis Ag Biologic compounds directed against death receptor 5
WO2011100841A1 (en) 2010-02-16 2011-08-25 Valorisation-Recherche, Limited Partnership Pd-1 modulation and uses thereof for modulating hiv replication
TW201134488A (en) 2010-03-11 2011-10-16 Ucb Pharma Sa PD-1 antibodies
JP2014501515A (ja) 2010-12-01 2014-01-23 グラクソ グループ リミテッド 改良された抗血清アルブミン結合単一可変ドメイン
EP2761008A1 (en) 2011-09-26 2014-08-06 Kymab Limited Chimaeric surrogate light chains (slc) comprising human vpreb
WO2013126712A1 (en) 2012-02-22 2013-08-29 The Trustees Of The University Of Pennsylvania Compositions and methods for generating a persisting population of t cells useful for the treatment of cancer
WO2013167883A1 (en) 2012-05-07 2013-11-14 The University Court Of The University Of Aberdeen Single domain binding molecule
EP2855526B1 (en) * 2012-05-24 2018-11-07 VIB vzw Anti-macrophage mannose receptor immunoglobulin single variable domains for targeting and in vivo imaging of tumor-associated macrophages
CN103087171B (zh) 2012-12-24 2015-01-14 中国人民解放军第四军医大学 一种用于前列腺癌早期诊断和治疗的抗psma/fitc双特异性抗体及其制备方法
US10993420B2 (en) 2013-03-15 2021-05-04 Erasmus University Medical Center Production of heavy chain only antibodies in transgenic mammals
CN103333249A (zh) 2013-06-14 2013-10-02 广州康合生物科技有限公司 一种抗前列腺特异性膜抗原（psma）的单克隆抗体及其应用
WO2015034820A1 (en) 2013-09-04 2015-03-12 Bristol-Myers Squibb Company Compounds useful as immunomodulators
JOP20200094A1 (ar) 2014-01-24 2017-06-16 Dana Farber Cancer Inst Inc جزيئات جسم مضاد لـ pd-1 واستخداماتها
KR20150089702A (ko) 2014-01-28 2015-08-05 제일모직주식회사 신규 화합물, 신규 폴리머, 이를 포함하는 착색제, 이를 포함하는 감광성 수지 조성물 및 컬러필터
JP2017505773A (ja) 2014-01-28 2017-02-23 ブリストル−マイヤーズ スクイブ カンパニーＢｒｉｓｔｏｌ−Ｍｙｅｒｓ Ｓｑｕｉｂｂ Ｃｏｍｐａｎｙ 血液悪性腫瘍を処置するための抗ｌａｇ−３抗体
JP2017513818A (ja) 2014-03-15 2017-06-01 ノバルティス アーゲー キメラ抗原受容体を使用する癌の処置
TWI754319B (zh) 2014-03-19 2022-02-01 美商再生元醫藥公司 用於腫瘤治療之方法及抗體組成物
JP6629187B2 (ja) 2014-04-07 2020-01-15 中外製薬株式会社 免疫活性化抗原結合分子
MA39378B2 (fr) 2014-04-25 2021-02-26 Pf Medicament Anticorps igf-1r et son utilisation comme véhicule d'adressage pour le traitement du cancer
US10092645B2 (en) * 2014-06-17 2018-10-09 Medimmune Limited Methods of treatment with antagonists against PD-1 and PD-L1 in combination with radiation therapy
TWI693232B (zh) 2014-06-26 2020-05-11 美商宏觀基因股份有限公司 與pd-1和lag-3具有免疫反應性的共價結合的雙抗體和其使用方法
CA2957258C (en) 2014-08-05 2023-11-07 MabQuest SA Immunological reagents
EP3180359A1 (en) 2014-08-14 2017-06-21 Novartis AG Treatment of cancer using gfr alpha-4 chimeric antigen receptor
AU2014409276A1 (en) 2014-10-22 2017-04-06 Crescendo Biologics Limited Transgenic mice
WO2016073760A1 (en) 2014-11-05 2016-05-12 The Regents Of The University Of California Methods for stratifying non-responders to therapies that block pd1/pdl1 axis
EP3916017A1 (en) 2014-12-22 2021-12-01 PD-1 Acquisition Group, LLC Anti-pd-1 antibodies
JP6783797B2 (ja) 2015-05-04 2020-11-11 ピエリス ファーマシューティカルズ ゲーエムベーハー 抗がん融合ポリペプチド
LT3298030T (lt) * 2015-05-18 2023-02-27 Pieris Pharmaceuticals Gmbh Sulietas polipeptidas prieš vėžį
CN105061597B (zh) 2015-06-09 2016-04-27 北京东方百泰生物科技有限公司 一种抗pd-1的单克隆抗体及其获得方法
SG10201906059VA (en) 2015-07-30 2019-08-27 Macrogenics Inc Pd-1-binding molecules and methods of use thereof
CN106397592A (zh) 2015-07-31 2017-02-15 苏州康宁杰瑞生物科技有限公司 针对程序性死亡配体(pd-l1)的单域抗体及其衍生蛋白
CN105384825B (zh) 2015-08-11 2018-06-01 南京传奇生物科技有限公司 一种基于单域抗体的双特异性嵌合抗原受体及其应用
CN108271377B (zh) 2015-10-07 2021-11-19 豪夫迈·罗氏有限公司 具有针对共刺激性tnf受体的四价的双特异性抗体
KR102220275B1 (ko) 2015-11-18 2021-02-26 머크 샤프 앤드 돔 코포레이션 Pd1 및/또는 lag3 결합제
MX2018008308A (es) 2016-01-11 2019-05-15 Inhibrx Inc Proteinas de fusion de union a 41bb multivalentes y multiespecificas.
US11236174B2 (en) * 2016-01-12 2022-02-01 Crescendo Biologics Limited Therapeutic molecules
CN105968205B (zh) 2016-02-03 2019-04-26 中国人民解放军第三军医大学第三附属医院 一种抗前列腺特异性膜抗原的纳米抗体
CN105968204B (zh) 2016-02-03 2020-01-21 中国人民解放军第三军医大学第一附属医院 一种抗前列腺特异性膜抗原的单域重链抗体
CN105968203A (zh) 2016-02-03 2016-09-28 南昌大学 一种抗前列腺特异性膜抗原胞外区的单域重链抗体
EP3445788B1 (en) 2016-04-22 2022-01-19 Alligator Bioscience AB Novel bispecific polypeptides against cd137
GB201607968D0 (en) 2016-05-06 2016-06-22 Crescendo Biolog Ltd Chimeric antigen receptor
IL263102B2 (en) 2016-05-20 2023-11-01 Harpoon Therapeutics Inc A serum albumin-binding protein with a single site
TW201806972A (zh) 2016-05-27 2018-03-01 美商艾伯維生物醫療股份有限公司 雙特異性結合蛋白
BR112019011189A2 (pt) 2016-12-07 2019-10-08 Ablynx Nv domínios variáveis únicos de imunoglobulina que se ligam à albumina sérica melhorada
CN107674122A (zh) 2016-12-28 2018-02-09 天津天锐生物科技有限公司 一种识别人血清白蛋白的单域抗体
JP7183163B2 (ja) 2017-01-06 2022-12-05 クレシェンド・バイオロジックス・リミテッド プログラム細胞死（ｐｄ－１）に対するシングルドメイン抗体
EP3579879A4 (en) 2017-02-09 2020-12-16 Memorial Sloan Kettering Cancer Center ANTI-BODY ANTI-KIR3DL1
WO2018224439A1 (en) 2017-06-05 2018-12-13 Numab Innovation Ag Novel anti-hsa antibodies
EP3425763B1 (en) 2017-07-03 2020-11-18 France Brevets Coupling interface and method of operation
EP3649156A1 (en) 2017-07-06 2020-05-13 Merus N.V. Antibodies that modulate a biological activity expressed by a cell
GB201711068D0 (en) 2017-07-10 2017-08-23 Crescendo Biologics Ltd Therapeutic molecules binding PSMA
JP7442443B2 (ja) * 2017-10-10 2024-03-04 ヌマブ セラピューティクス アクチェンゲゼルシャフト 多重特異性抗体
EP3470426A1 (en) 2017-10-10 2019-04-17 Numab Therapeutics AG Multispecific antibody
KR20200083574A (ko) 2017-11-13 2020-07-08 크레센도 바이오로직스 리미티드 Cd137 및 psma에 결합하는 분자
GB201802573D0 (en) 2018-02-16 2018-04-04 Crescendo Biologics Ltd Therapeutic molecules that bind to LAG3
GB201818460D0 (en) 2018-11-13 2018-12-26 Crescendo Biologics Ltd Single domain antibodies that bind human serum albumin
SG11202111671QA (en) 2019-05-15 2021-11-29 Crescendo Biologics Ltd Binding molecules

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
Casset et al. (BBRC 2003, 307:198-205) (Year: 2003) *
Chen et al. (J. Mol. Bio. (1999) 293, 865-881) (Year: 1999) *
Lamminmaki et al. (JBC 2001, 276:36687-36694) (Year: 2001) *
MacCallum et al. J. Mol. Biol. (1996) 262, 732-745 (Year: 1996) *
Padlan et al. (PNAS 1989, 86:5938-5942) (Year: 1989) *
Pascalis et al. (The Journal of Immunology (2002) 169, 3076-3084) (Year: 2002) *
Rudikoff et al. (Proc Natl Acad Sci USA 1982 Vol 79 page 1979) (Year: 1979) *
Vajdos et al. (J. Mol. Biol. (2002) 320, 415-428) (Year: 2002) *
Wu et al. (J. Mol. Biol. (1999) 294, 151-162) (Year: 1999) *

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11236174B2 (en) 2016-01-12 2022-02-01 Crescendo Biologics Limited Therapeutic molecules
US11746158B2 (en) 2016-01-12 2023-09-05 Crescendo Biologics Limited Therapeutic molecules
US11866510B2 (en) 2016-05-06 2024-01-09 Crescendo Biologics Limited Chimeric antigen receptor with single domain antibody
US11591398B2 (en) 2017-01-06 2023-02-28 Crescendo Biologics Limited Single domain antibodies to programmed cell death protein 1 (PD-1)
US11814429B2 (en) 2017-01-06 2023-11-14 Crescendo Biologics Limited Single domain antibodies to programmed cell death (PD-1)
US11401342B2 (en) * 2017-07-10 2022-08-02 Crescendo Biologics Limited Therapeutic molecules binding PSMA
US12077595B2 (en) 2017-11-13 2024-09-03 Crescendo Biologics Limited Single domain antibodies that bind to CD137
US11951172B2 (en) 2018-02-16 2024-04-09 Crescendo Biologics Limited Therapeutic molecules that bind to LAG3 and PD1
CN113621065A (zh) * 2021-08-30 2021-11-09 武汉海沙百得生物技术有限公司 靶向4-1bb的全人源抗体及其制备方法和应用
WO2023030258A1 (en) * 2021-08-31 2023-03-09 LaNova Medicines Limited Anti-4-1bb nanobodies

Also Published As

Publication number Publication date
KR20200083574A (ko) 2020-07-08
US20200362047A1 (en) 2020-11-19
EP3710477A1 (en) 2020-09-23
CN111699197B (zh) 2024-08-23
EP3710478A1 (en) 2020-09-23
WO2019092452A1 (en) 2019-05-16
JP7312168B2 (ja) 2023-07-20
AU2018363292A1 (en) 2020-05-21
US12077595B2 (en) 2024-09-03
KR20200080304A (ko) 2020-07-06
CN111699197A (zh) 2020-09-22
CA3082321A1 (en) 2019-05-16
IL274370A (en) 2020-06-30
CN118667027A (zh) 2024-09-20
CN111683968B (zh) 2024-07-05
AU2018363291A1 (en) 2020-05-21
JP2021502810A (ja) 2021-02-04
WO2019092451A1 (en) 2019-05-16
CA3082297A1 (en) 2019-05-16
JP2021502104A (ja) 2021-01-28
JP2023126951A (ja) 2023-09-12
IL274371A (en) 2020-06-30
SG11202003912RA (en) 2020-05-28
SG11202003978UA (en) 2020-05-28
CN111683968A (zh) 2020-09-18

Similar Documents

Publication Publication Date Title
US20200362051A1 (en) Molecules that bind to cd137 and psma
CN110036032B (zh) 抗muc16(粘蛋白16)抗体
US20220306744A1 (en) Single Domain Antibodies to Programmed Cell Death (PD-1)
KR102587941B1 (ko) 향상된 효능작용 및 이펙터 기능을 갖는 조작된 항체 및 다른 Fc-도메인 함유 분자
JP6794348B2 (ja) ヒト化抗ｏｘ４０抗体及びその使用
JP6515101B2 (ja) Ｇｉｔｒ抗原結合タンパク質
JP2021006044A (ja) 腫瘍壊死因子 （ｔｎｆ） スーパーファミリー受容体結合分子およびその使用
CN109641037B (zh) 抗psma抗体及其用途
JP2014518640A (ja) β−クロトおよびＦＧＦ受容体を含む複合体に結合するヒト抗原結合タンパク質
US20220220215A1 (en) Binding molecules
US20240002530A1 (en) Anti-psma antibodies and methods of use
WO2023173393A1 (zh) 结合b7-h3的抗体及其用途
US20230183366A1 (en) Recombinant proteins with ox40 activating properties
WO2024188272A1 (zh) Trop2单域抗体、融合蛋白及其应用

Legal Events

Date Code Title Description
AS Assignment

Owner name: CRESCENDO BIOLOGICS LIMITED, UNITED KINGDOM

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:BRUCKLACHER-WALDERT, VERENA;EDWARDS, CAROLYN;LEGG, JAMES;AND OTHERS;SIGNING DATES FROM 20200520 TO 20200706;REEL/FRAME:053386/0863

STPP Information on status: patent application and granting procedure in general

Free format text: APPLICATION DISPATCHED FROM PREEXAM, NOT YET DOCKETED

STPP Information on status: patent application and granting procedure in general

Free format text: DOCKETED NEW CASE - READY FOR EXAMINATION

STPP Information on status: patent application and granting procedure in general

Free format text: NON FINAL ACTION MAILED

STPP Information on status: patent application and granting procedure in general

Free format text: NON FINAL ACTION MAILED

STPP Information on status: patent application and granting procedure in general

Free format text: RESPONSE TO NON-FINAL OFFICE ACTION ENTERED AND FORWARDED TO EXAMINER

STPP Information on status: patent application and granting procedure in general

Free format text: FINAL REJECTION MAILED

STPP Information on status: patent application and granting procedure in general

Free format text: DOCKETED NEW CASE - READY FOR EXAMINATION

STPP Information on status: patent application and granting procedure in general

Free format text: NON FINAL ACTION MAILED

STPP Information on status: patent application and granting procedure in general

Free format text: RESPONSE TO NON-FINAL OFFICE ACTION ENTERED AND FORWARDED TO EXAMINER

STPP Information on status: patent application and granting procedure in general

Free format text: FINAL REJECTION MAILED