US20080175952A1 - Fermented food products containing probiotic strains and method for preparing same - Google Patents

Fermented food products containing probiotic strains and method for preparing same Download PDF

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Publication number
US20080175952A1
US20080175952A1 US12/013,636 US1363608A US2008175952A1 US 20080175952 A1 US20080175952 A1 US 20080175952A1 US 1363608 A US1363608 A US 1363608A US 2008175952 A1 US2008175952 A1 US 2008175952A1
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Prior art keywords
food product
fermented food
stage
substance
bifidobacteria
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Abandoned
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US12/013,636
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English (en)
Inventor
Luc Terragno
Francois DEBRU
Philippe Teissier
Stephane HERVE
Jean-Luc BLACHON
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Gervais Danone SA
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Gervais Danone SA
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Assigned to COMPAGNIE GERVAIS DANONE reassignment COMPAGNIE GERVAIS DANONE ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: BLACHON, JEAN-LUC, DEBRU, FRANCOIS, TERRAGNO, LUC, STEPHANE, HERVE, TESSIER, PHILIPPE
Publication of US20080175952A1 publication Critical patent/US20080175952A1/en
Priority to US13/706,624 priority Critical patent/US9119415B2/en
Abandoned legal-status Critical Current

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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C11/00—Milk substitutes, e.g. coffee whitener compositions
    • A23C11/02—Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins
    • A23C11/10—Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins containing or not lactose but no other milk components as source of fats, carbohydrates or proteins
    • A23C11/103—Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins containing or not lactose but no other milk components as source of fats, carbohydrates or proteins containing only proteins from pulses, oilseeds or nuts, e.g. nut milk
    • A23C11/106—Addition of, or treatment with, microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/123—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt
    • A23C9/1234—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt characterised by using a Lactobacillus sp. other than Lactobacillus Bulgaricus, including Bificlobacterium sp.
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L11/00—Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
    • A23L11/60—Drinks from legumes, e.g. lupine drinks
    • A23L11/65—Soy drinks
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/02—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/51—Bifidobacterium
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/51—Bifidobacterium
    • A23V2400/515—Animalis

Definitions

  • the invention relates to fermented food products containing probiotic strains, and their preparation process.
  • the bifidobacteria belong to the dominant anaerobic flora in the colon.
  • the main species present in the human colon are Bifidobacterium adolescentis, Bifidobacterium bifidum, Bifidobacterium longum ssp infantis, Bifidobacterium breve, Bifidobacterium longum.
  • the bifidobacteria are probiotic bacteria of choice. Bacteria of the genus Bifidobacterium are used in numerous products currently on the market and are often added to dairy products already comprising the standard bacteria in yoghurt ( Streptococcus thermophilus and Lactobacillus bulgaricus ).
  • Probiotics are live bacteria. The use of these live bacteria in the manufacture of food products such as dairy products is tricky in particular with regard to the problem of survival of these bacteria in the product.
  • the main purpose of the invention is to provide non-firm fermented food products containing a high population of bifidobacteria throughout the period of preservation of said fermented food products, i.e. non-firm fermented food products containing bifidobacteria in a good physiological state and having a significant survival rate during the period of preservation of said fermented food products, in particular until the use-by date of the products.
  • Another purpose of the invention is to provide preparation processes which are simple to implement, making it possible to obtain the above products.
  • Another purpose of the invention is to promote the growth of the bifidobacteria over the standard symbioses present in yoghurts, these symbioses being constituted in a standard fashion by one or more strains of Streptococcus thermophilus and of Lactobacillus bulgaricus.
  • the purposes of the invention are achieved thanks to the surprising finding made by the inventors that a precise regulation of a certain number of parameters involved in a standard fashion in the processes for the preparation of non-firm fermented food products makes it possible to obtain rapidly, after fermentation of the populations, at least 10 8 bifidobacteria per gram of product, and increased survival of the bifidobacteria up to the use-by date of the products, without necessarily modifying the growth of the other bacterial strains.
  • the invention relates to a non-firm fermented food product containing ferments, which contain lactic bacteria, comprising more than approximately 5 ⁇ 10 7 , in particular more than approximately 10 8 bifidobacteria per gram of food product fermented for a preservation period of at least 30 days, in particular at least 35 days.
  • non-firm fermented food product is meant a fermented food product which has undergone a stage of cutting of coagulum and/or smoothing during its preparation process prior to its packaging.
  • a firm fermented food product is a product which is packaged before the fermentation, the fermentation occurring in the packaging container.
  • the milk is seeded and directly packaged in pots where it ferments. After the seeding, the seeded milk is packaged in pots. These pots are generally placed in an oven for 3 hours.
  • the bacteria reproduce and consume the lactose which is then partially converted to lactic acid which modifies the structure of the proteins, forming what is known as a lactic gel.
  • the products are placed in a ventilated cooler or cooling tunnel and stored at approximately 4° C.
  • non-firm fermented food products examples include stirred yoghurts or drinking yoghurts.
  • kits is meant a set of bacteria, in particular bacteria intended for to fermentation and/or bacteria with probiotic value.
  • the preservation or storage period of the fermented food product is the period which immediately follows the end of the process of preparation of the fermented food product and its packaging. During this preservation period the fermented food product is usually preserved at a temperature comprised between approximately 4 and approximately 10° C.
  • the abovementioned fermented food product contains more than approximately 5 ⁇ 10 7 , in particular more than approximately 10 8 bifidobacteria per gram of fermented food product in particular for a preservation period of at least 35 days, and more particularly for a preservation period of at least 40 days. More particularly the abovementioned fermented food product contains more than approximately 5 ⁇ 10 7 , in particular more than approximately 10 8 bifidobacteria per gram of fermented food product up to the use-by date of the product.
  • the use-by dates depend on the legal preservation periods fixed by current legislation, which can typically vary from 15 to 50 days from the date of production.
  • the legal preservation period is generally 30 days for fresh dairy products.
  • a population of bifidobacteria which is greater than or equal to 10 8 CFU/g at the use-by date of product preserved between 4 and 10° C. can be considered as a sufficient population of bifidobacteria given the medical recommendations relating to the provision of bifidobacteria in food.
  • the invention relates more particularly to the fermented food product as defined above, in which the ratio of the number of bifidobacteria contained in the fermented food product at the end of the preservation period to the number of bifidobacteria contained in the fermented food product at the start of the preservation period of at least 30 days, in particular at least 35 days, is approximately 0.2 to approximately 0.8, in particular approximately 0.3 to approximately 0.7, in particular approximately 0.4 to approximately 0.5.
  • the survival rate of the bifidobacteria contained in the fermented food product between the start of the preservation period (i.e. the end of the preparation process) and the end of the preservation period is comprised between 20 and 80%, in particular between 30 and 70%, and in particular between 40 and 50%.
  • Said preservation period is at least 30 days, in particular at least 35 days, but more particularly at least 40 days or extends up to the use-by date of the fermented food product.
  • the invention also relates to a non-firm fermented food product preserved for a preservation period of at least 30 days, in particular at least 35 days, at a temperature of approximately 4 to approximately 10° C., containing ferments comprising more than approximately 10 8 bifidobacteria per gram of fermented food product.
  • the invention relates to a non-firm fermented food product preserved for a preservation period of at least 30 days, in particular at least 35 days, in particular at least 40 days, at a temperature of less than 12° C. or less than 10° C., containing ferments comprising more than approximately 5 ⁇ 10 7 , in particular more than approximately 10 8 bifidobacteria per gram of fermented food product.
  • the invention relates more particularly to a fermented food product as defined above, containing more than approximately 5 ⁇ 10 7 , in particular more than approximately 10 8 bifidobacteria per gram of fermented food product at the start of the preservation period.
  • Fermented food product according to one of claims 1 to 4 , in which the bifidobacteria contained in the fermented food product are of the type Bifidobacterium animalis , in particular Bifidobacterium animalis animalis and/or Bidifobacterium animalis lactis , and/or Bifidobacterium breve and/or Bifidobacterium longum and/or Bidifobacterium infantis and/or Bidifobacterium bifidum.
  • the fermented food product as defined above is prepared based on plant juice and in particular fruit juice or vegetable juice such as soya juice, or on a dairy product, and in particular on cow's milk and/or on goat's milk.
  • Said fermented food product can also be based on sheep's milk or on camel's milk or mare's milk.
  • plant juice is meant a juice produced from plant extracts, in particular soya, tonyu, oat, wheat, maize etc.
  • Examples of vegetable juice are: tomato juice, beet juice, carrot juice etc.
  • fruit juice examples include apple, orange, strawberry, peach, apricot, plum, raspberry, blackberry, gooseberry, pineapple, lemon, citrus fruit, grapefruit, banana, kiwi fruit, pear, cherry, passion fruit, mango, exotic fruit juice, multifruit juice etc.
  • the fermented food product as defined above is such that the ferments contain lactic bacteria, in particular one or more bacteria of the genus Lactobacillus spp. and in particular Lactobacillus delbrueckii bulgaricus and/or Lactobacillus casei and/or Lactobacillus reuteri and/or Lactobacillus acidophilus and/or Lactobacillus helveticus and/or Lactobacillus plactarum , and/or bacteria of the type Lactococcus cremoris and/or Streptococcus thermophilus and/or Lactococcus locus and/or one or more bacteria of the genus Leuconostoc.
  • the fermented food product as defined above is such that the ferments contain lactic bacteria which exhibit a symbiotic phenomenon between themselves.
  • symbiotic phenomenon is meant a relation between different types of lactic bacteria which are capable of creating a mutual assistance between themselves and increasing their fermentation activities.
  • the proportion of bifidobacteria in the ferments contained in the fermented food product as defined above is approximately 20 to approximately 80%, in particular approximately 30 to approximately 70%, in particular approximately 40 to approximately 60%, and in particular approximately 50%.
  • proportion of bifidobacteria in the ferments is meant the proportion of bifidobacteria relative to the total number of bacteria included in the fermented food product, i.e. relative to all of the bifidobacteria and other bacteria, in particular the bacteria Lactococcus, Lactobacillus, Streptococcus etc.
  • a proportion of 50% bifidobacteria constitutes a good compromise between the problem of cost (the bifidobacteria are expensive) and the problem of obtaining a correct population of bifidobacteria.
  • the fermented food product according to the invention is presented in the form of a stirred fermented food product or a fermented food product for drinking or an infant fermented food product.
  • stirred [ . . . ] product is meant a product, in particular a milk, seeded, fermented, mechanically stirred then packaged. The fermentation of such a product is carried out not in a pot but in bulk, in tanks. The curd is stirred then cooled down before being packed in pots, which are stored under refrigeration. By curd is meant a coagulate of proteins in particular of milk.
  • product for drinking is meant a product in substantially liquid form.
  • a product for drinking is a product which is such that, after the mechanical stirring stage, the product is beaten in the tanks before being packaged.
  • infant [ . . . ] product is meant a product suited to an infant's needs, with a low protein and fat content.
  • Said fermented food product can in particular be a yoghurt or a firm, stirred or drinking yoghurt or a bar containing a dairy substance, kefir, a biscuit with a dairy filling, a water containing probiotics etc.
  • the invention also relates to a process for the preparation of a fermented food product from a starting substance, comprising the following successive stages:
  • a stage of final cooling of the stored substance obtained in the preceding stage such that the temperature at the start of the final cooling is less than approximately 21° C. and the temperature at the end of the final cooling is approximately 2 to approximately 6° C., so as to obtain a fermented food product.
  • the ferments used for seeding the starting substance are generally obtained by growing bifidobacteria in a culture medium and under conditions such that when the population of bifidobacteria arrives at confluence, it contains 10 8 to 10 9 bifidobacteria per ml of culture medium. It is therefore noted that according to the invention the quantity of ferments used at the start corresponds to approximately 0.1% of the dose of bifidobacteria used in a standard fashion.
  • reaction is meant a biochemical reaction which involves releasing energy from an organic substrate, under the action of micro-organisms. It is a process of conversion of a raw material by the micro-organisms, this conversion then producing biomass and metabolites.
  • lactic fermentation is an anaerobic process of the consumption of lactose by the bacteria in the ferments, which causes the formation of lactic acid and a lowering of the pH.
  • the invention follows from the surprising finding made by the inventors that the regulation of the parameters of time, temperature and initial population of bifidobacteria within the abovementioned ranges, makes it possible to improve the resistance of the bifidobacteria and their ability to survive.
  • the bidifobacteria contained in the fermented food product at the end of the preparation process of the invention are in a better physiological state than if said parameters are fixed at values situated outside the above ranges, which allows a greater number of these bifidobacteria to survive during the preservation of the fermented food product which follows.
  • the survival rate of the bifidobacteria during preservation is unfavorably altered.
  • the abovementioned preparation process makes it possible to obtain non-firm fermented food products.
  • the ferments contain lactic bacteria.
  • the process for the preparation of a fermented food product according to the invention is such that the bifidobacteria are chosen from bacteria of the type Bifidobacterium animalis , in particular Bifidobacterium animalis animalis and/or Bidifobacterium animalis lactis , and/or Bifidobacterium breve and/or Bifidobacterium longum and/or Bifidobacterium infantis and/or Bifidobacterium bifidum.
  • the process for the preparation of a fermented food product according to the invention is such that the bifidobacteria are chosen from bacteria of the type Bifidobacterium animalis.
  • the intermediate cooling time in the process for the preparation of a fermented food product according to the invention is approximately 1 hour 30 minutes.
  • the storage time in the process for the preparation of a fermented food product according to the invention is less than or equal to approximately 12 hours, in particular equal to approximately 12 hours.
  • the temperature at the end of final cooling in the process for the preparation of a fermented food product according to the invention is approximately 4° C.
  • the seeding ferments contain lactic bacteria, in particular one or more bacteria of the genus Lactobacillus spp. and in particular Lactobacillus delbrueckii bulgaricus and/or Lactobacillus casei and/or Lactobacillus reuteri and/or Lactobacillus acidophilus and/or Lactobacillus helveticus and/or Lactobacillus plantarum , and/or bacteria of the type Lactococcus cremoris and/or Streptococcus thermophilus and/or Lactococcus lactis and/or one or more bacteria of the genus Leuconostoc.
  • the lactic bacteria exhibit a symbiotic phenomenon between themselves.
  • the proportion of bifidobacteria in the seeding ferments is approximately 20 to approximately 75%, in particular approximately 30 to approximately 50%, in particular approximately 35 to approximately 40%, in particular approximately 37.5%.
  • proportion of the bifidobacteria in the seeding ferments is meant the proportion of the bifidobacteria relative to all of the inoculated bacteria in total during the seeding stage.
  • This proportion corresponds to an optimum in terms of cost and final concentration of bifidobacteria, given that the higher the concentration of bifidobacteria at the start, the more competitive they are in terms of growth relative to the other strains in the ferments, and the more rapidly the optimum concentration of bifidobacteria is reached.
  • the starting substance is based on plant juice and in particular fruit juice or vegetable juice such as soya juice, or on a dairy product, constituted in particular by cow's milk and/or goat's milk.
  • the starting substance can also comprise sheep's and/or camel's and/or mare's milk.
  • the starting substance can comprise milk, milk powder, sugar, a mixture of milk and plant juice, a mixture of milk and fruit juice, a mixture of milk and starch.
  • the process for the preparation of a fermented food product according to the invention comprises an additional stage of stirring between the fermentation stage and the intermediate cooling stage, making it possible to obtain, from the fermented substance obtained in the fermentation stage, a stirred fermented substance.
  • stir is meant a process of mechanical stirring using a turbine or helical stirrer. It is a stage which determines the oiliness of the product in particular the dairy product. If the stirring is too violent, incorporation of air and separation of the serum can occur. If the stirring is insufficient, the product risks subsequently becoming too thick.
  • the process for the preparation of a fermented food product according to the invention comprises a pasteurization stage before the seeding stage, making it possible to obtain, from the starting substance, a pasteurized starting substance.
  • pasteurization is meant the method usual in the field of food preservation involving a rapid heating without boiling, followed by rapid cooling, making it possible to destroy most of the bacteria while partially preserving the proteins.
  • the pasteurized starting substance is a pasteurized starting substance, which is held, optionally homogenized, and cooled down, obtained from a raw material, said process comprising, before the seeding stage, the following successive stages:
  • standardization of fatty substances is meant a stage of bringing the quantity of fats present in the starting substance to a pre-determined level.
  • Enrichment with dried matter involves the addition of proteins and fatty substance in order to modify the firmness of the curd.
  • “Holding” involves a rapid thermization of the milk and makes it possible to destroy the vegetative microbial flora, including pathogenic forms. Its typical duration is from 4 to 10 minutes, in particular from 5 to 8 minutes, and in particular approximately 6 minutes.
  • homogenization is meant the dispersion of the fatty substances in the milk-type substance into small fat globules.
  • the homogenization is carried out for example at a pressure of 100 to 280 bars, in particular 100 to 250 bars, in particular 100 to 200 bars, in particular approximately 200 bars.
  • This homogenization stage is purely optional. It is in particular absent from the production process of products with 0% fatty substances.
  • the process for the preparation of a fermented food product according to the invention comprises, after the final cooling stage, a stage of preservation of the fermented food product at a temperature comprised between approximately 4 and approximately 10° C.
  • the process for the preparation of a fermented food product according to the invention comprises a stage of the addition of an intermediate preparation simultaneously with the seeding stage or between the seeding stage and the fermentation stage, so as to obtain, from the seeded substance, a completed seeded substance, or after the fermentation stage, so as to obtain, from the fermented substance, a completed fermented substance, said intermediate preparation comprising a preparation of fruits and/or cereals and/or additives such as flavourings and colourings.
  • the intermediate preparation can in particular contain thickeners (soluble and insoluble fibres, alginates, carragheenans, xanthan gum, pectin, starch, in particular gelatinized, gelan gum, cellulose and its derivatives, guar and carob gum, inulin) or sweeteners (aspartame, acesulphame K, saccharine, sucralose, cyclamate) or preservatives.
  • thickeners soluble and insoluble fibres, alginates, carragheenans, xanthan gum, pectin, starch, in particular gelatinized, gelan gum, cellulose and its derivatives, guar and carob gum, inulin
  • sweeteners aspartame, acesulphame K, saccharine, sucralose, cyclamate
  • flavourings are: apple, orange, strawberry, kiwi fruit, cocoa flavouring etc.
  • colourings are: beta-carotene, carmine, cochineal red.
  • the preparation of the abovementioned fruits can comprise fruits which are whole or in pieces or in jelly or in jam, making it possible for example to obtain fruit yoghurts.
  • the intermediate preparation can also contain plant extracts (soya, rice etc.).
  • the invention also relates to a fermented food product as obtained from one of the processes as defined above.
  • FIG. 1 represents the tolerance chart corresponding to the effect of the fermentation temperature as a function of the quantity of bifidobacteria at the start.
  • x-axis quantity of bifidobacteria inoculated at 10 7 CFU/ml; y-axis: fermentation temperature in degrees celsius.
  • Black quantity of bifidobacteria less than 5 ⁇ 10 7 CFU/ml; fine hatching: quantity of bifidobacteria comprised between 5 ⁇ 10 7 and 10 ⁇ 8 CFU/ml; thick hatching: quantity of bifidobacteria greater than 10 8 CFU/ml.
  • the measurement is carried out at the end of fermentation.
  • FIG. 2 represents the tolerance chart corresponding to the effect of the cooling temperature of the products before the final storage at 10° C. as a function of the waiting time before cooling to 20° C.
  • x-axis waiting time for smoothing (in hours);
  • y-axis cooling temperature before final storage (in ° C.).
  • Fine hatching quantity of bifidobacteria comprised between 5 ⁇ 10 7 and 10 8 CFU/ml; thick hatching: quantity of bifidobacteria greater than 10 8 CFU/ml. The measurement is carried out 35 days after fermentation.
  • FIG. 3 represents the tolerance chart corresponding to the effect of the cooling temperature of the products before final storage at 10° C. as a function of the fermentation temperature.
  • x-axis fermentation temperature (° C.);
  • y-axis cooling temperature during final storage (° C.).
  • Black quantity of bifidobacteria less than 5 ⁇ 10 7 CFU/ml; fine hatching: quantity of bifidobacteria comprised between 5 ⁇ 10 7 and 10 8 CFU/ml; thick hatching: quantity of bifidobacteria greater than 10 8 CFU/ml.
  • the measurement is carried out 35 days after the fermentation.
  • FIG. 2 it is noted that if the products are not cooled sufficiently rapidly (too long a waiting time in fermentation tank), there is a loss of bifidobacteria.
  • FIG. 3 it is noted that if the products are not cooled correctly between 4° C. and 6° C., the loss in bifidobacteria at D+35 is significant.
  • Control 1 fermentation temperature 39° C.; storage time 24 h.
  • Control 2 fermentation temperature 40° C.
  • Test 1 fermentation temperature 37° C., cooling 6° C.
  • control 1 and control 2 milk+milk powder
  • Control 3 fermentation temperature 39° C.
  • Control 4 fermentation temperature 40° C.
  • Test 2 all the parameters are chosen from the claimed ranges, i.e.:
  • control 3 and control 4 milk+milk powder+fruit preparation added at the end of production
  • D corresponds to the end of fermentation
  • D+1 corresponds to storage for 1 day etc.

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FR0507528A FR2888468B1 (fr) 2005-07-13 2005-07-13 Produits alimentaires fermentes contenant des souches probiotiques, et leur procede de preparation
FR05/07528 2005-07-13
PCT/FR2006/001687 WO2007006969A1 (fr) 2005-07-13 2006-07-11 Produits alimentaires fermentes contenant des souches probiotiques, et leur procede de preparation

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US20130302454A1 (en) * 2011-01-21 2013-11-14 Jilin Zixin Pharmaceutical Research Institution Llc. Method for preparing fermentation broth of fruits and vegetables
WO2015120100A1 (en) * 2014-02-04 2015-08-13 Micro-Nature Llc Systems, methods, and compositions for promoting pathogen control and food preservation
CN111349656A (zh) * 2019-12-31 2020-06-30 刘国栋 酵素及其生产方法和应用
CN114304270A (zh) * 2022-01-10 2022-04-12 光明乳业股份有限公司 一种发酵乳饮品及其制备方法

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CN105007745A (zh) * 2013-01-25 2015-10-28 热尔韦法国达能公司 制备脱乳清发酵乳制品的方法
CN103555598B (zh) * 2013-11-04 2016-01-20 西藏月王生物技术有限公司 一种食用酵素复合菌剂及其制备方法
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CN107996706A (zh) * 2017-12-26 2018-05-08 光明乳业股份有限公司 一种富含短双歧杆菌的发酵乳及其制备方法
CN107996705A (zh) * 2017-12-26 2018-05-08 光明乳业股份有限公司 一种富含长双歧杆菌的发酵乳及其制备方法
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Cited By (10)

* Cited by examiner, † Cited by third party
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WO2012078046A1 (en) * 2010-12-10 2012-06-14 N.V. Nutricia Early stimulation of taste and/or flavour acceptance
WO2012078032A1 (en) * 2010-12-10 2012-06-14 N.V. Nutricia Early stimulation of taste and/or flavour acceptance
CN103354722A (zh) * 2010-12-10 2013-10-16 N·V·努特里奇亚 对口味和/或风味接受度的早期刺激
US8859029B2 (en) 2010-12-10 2014-10-14 N.V. Nutricia Early stimulation of taste and/or flavour acceptance
US9408409B2 (en) 2010-12-10 2016-08-09 N.V. Nutricia Early stimulation of taste and/or flavour acceptance
US20130302454A1 (en) * 2011-01-21 2013-11-14 Jilin Zixin Pharmaceutical Research Institution Llc. Method for preparing fermentation broth of fruits and vegetables
US9427452B2 (en) * 2011-01-21 2016-08-30 Jilin Zixin Pharmaceutical Research Institution Llc. Method for preparing fermentation broth of fruits and vegetables
WO2015120100A1 (en) * 2014-02-04 2015-08-13 Micro-Nature Llc Systems, methods, and compositions for promoting pathogen control and food preservation
CN111349656A (zh) * 2019-12-31 2020-06-30 刘国栋 酵素及其生产方法和应用
CN114304270A (zh) * 2022-01-10 2022-04-12 光明乳业股份有限公司 一种发酵乳饮品及其制备方法

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CN101262775B (zh) 2013-08-07
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BRPI0613529A2 (pt) 2011-01-18
RU2008105401A (ru) 2009-08-20
PL1901612T3 (pl) 2017-07-31
MX2008000508A (es) 2008-03-06
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CA2615041C (fr) 2015-03-17
US20130095208A1 (en) 2013-04-18
ES2605416T3 (es) 2017-03-14
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PT1901612T (pt) 2016-11-17
ZA200800361B (en) 2009-05-27
BRPI0613529B1 (pt) 2018-07-31
US9119415B2 (en) 2015-09-01
WO2007006969A1 (fr) 2007-01-18
JP2009501016A (ja) 2009-01-15
EP1901612A1 (fr) 2008-03-26
JP5491027B2 (ja) 2014-05-14
CN101262775A (zh) 2008-09-10
JP2014087362A (ja) 2014-05-15
UA96271C2 (ru) 2011-10-25
CA2615041A1 (fr) 2007-01-18

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