UA72421C2 - A process for dna purifying by formation of triple helix with immobilized oligonucleotides (variants) - Google Patents
A process for dna purifying by formation of triple helix with immobilized oligonucleotides (variants) Download PDFInfo
- Publication number
- UA72421C2 UA72421C2 UA97062835A UA97062835A UA72421C2 UA 72421 C2 UA72421 C2 UA 72421C2 UA 97062835 A UA97062835 A UA 97062835A UA 97062835 A UA97062835 A UA 97062835A UA 72421 C2 UA72421 C2 UA 72421C2
- Authority
- UA
- Ukraine
- Prior art keywords
- sequence
- fact
- dna
- oligonucleotide
- plasmid
- Prior art date
Links
- 108091034117 Oligonucleotide Proteins 0.000 title claims abstract description 121
- 238000000034 method Methods 0.000 title claims abstract description 81
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 title description 28
- 230000015572 biosynthetic process Effects 0.000 title description 6
- 108020004414 DNA Proteins 0.000 claims abstract description 94
- 102000053602 DNA Human genes 0.000 claims abstract description 22
- 238000000746 purification Methods 0.000 claims abstract description 22
- 239000000203 mixture Substances 0.000 claims abstract description 18
- 239000013612 plasmid Substances 0.000 claims description 102
- 108090000623 proteins and genes Proteins 0.000 claims description 16
- 238000009396 hybridization Methods 0.000 claims description 15
- 239000006166 lysate Substances 0.000 claims description 10
- 239000013611 chromosomal DNA Substances 0.000 claims description 9
- 230000010076 replication Effects 0.000 claims description 8
- 125000004432 carbon atom Chemical group C* 0.000 claims description 7
- 239000003814 drug Substances 0.000 claims description 7
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 6
- 229910019142 PO4 Inorganic materials 0.000 claims description 6
- 229940079593 drug Drugs 0.000 claims description 6
- 101710163270 Nuclease Proteins 0.000 claims description 5
- 239000000969 carrier Substances 0.000 claims description 5
- 239000013592 cell lysate Substances 0.000 claims description 5
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 5
- 239000010452 phosphate Substances 0.000 claims description 5
- 238000004587 chromatography analysis Methods 0.000 claims description 4
- 108020004638 Circular DNA Proteins 0.000 claims description 3
- BWGNESOTFCXPMA-UHFFFAOYSA-N Dihydrogen disulfide Chemical compound SS BWGNESOTFCXPMA-UHFFFAOYSA-N 0.000 claims description 3
- 150000001408 amides Chemical class 0.000 claims description 2
- 238000007385 chemical modification Methods 0.000 claims description 2
- 239000004816 latex Substances 0.000 claims description 2
- 229920000126 latex Polymers 0.000 claims description 2
- 239000004033 plastic Substances 0.000 claims description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims 3
- 229910052799 carbon Inorganic materials 0.000 claims 3
- 229940126601 medicinal product Drugs 0.000 claims 1
- 239000000758 substrate Substances 0.000 abstract 1
- 239000000872 buffer Substances 0.000 description 45
- 239000002773 nucleotide Substances 0.000 description 30
- 125000003729 nucleotide group Chemical group 0.000 description 30
- 239000002299 complementary DNA Substances 0.000 description 24
- 239000000243 solution Substances 0.000 description 17
- 210000004027 cell Anatomy 0.000 description 12
- 230000000295 complement effect Effects 0.000 description 9
- 230000003993 interaction Effects 0.000 description 9
- 238000012986 modification Methods 0.000 description 9
- 230000004048 modification Effects 0.000 description 9
- 235000010469 Glycine max Nutrition 0.000 description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
- 238000010367 cloning Methods 0.000 description 8
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 8
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 7
- 238000010276 construction Methods 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 239000012634 fragment Substances 0.000 description 6
- SCVFZCLFOSHCOH-UHFFFAOYSA-M potassium acetate Chemical compound [K+].CC([O-])=O SCVFZCLFOSHCOH-UHFFFAOYSA-M 0.000 description 6
- 230000009466 transformation Effects 0.000 description 6
- ZRALSGWEFCBTJO-UHFFFAOYSA-N Guanidine Chemical compound NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 description 5
- 125000003277 amino group Chemical group 0.000 description 5
- 238000001962 electrophoresis Methods 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- 235000021317 phosphate Nutrition 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- 125000006850 spacer group Chemical group 0.000 description 5
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 4
- 239000011543 agarose gel Substances 0.000 description 4
- 238000004140 cleaning Methods 0.000 description 4
- 238000010790 dilution Methods 0.000 description 4
- 239000012895 dilution Substances 0.000 description 4
- 239000003550 marker Substances 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 108091008146 restriction endonucleases Proteins 0.000 description 4
- 239000011780 sodium chloride Substances 0.000 description 4
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 3
- 241000283725 Bos Species 0.000 description 3
- CPELXLSAUQHCOX-UHFFFAOYSA-M Bromide Chemical compound [Br-] CPELXLSAUQHCOX-UHFFFAOYSA-M 0.000 description 3
- 241000701022 Cytomegalovirus Species 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 244000068988 Glycine max Species 0.000 description 3
- UYTPUPDQBNUYGX-UHFFFAOYSA-N Guanine Natural products O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 3
- 108060001084 Luciferase Proteins 0.000 description 3
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 3
- 108010090804 Streptavidin Proteins 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 238000005859 coupling reaction Methods 0.000 description 3
- 229940104302 cytosine Drugs 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 102000039446 nucleic acids Human genes 0.000 description 3
- 108020004707 nucleic acids Proteins 0.000 description 3
- 150000007523 nucleic acids Chemical class 0.000 description 3
- 230000003287 optical effect Effects 0.000 description 3
- 239000002953 phosphate buffered saline Substances 0.000 description 3
- 238000003752 polymerase chain reaction Methods 0.000 description 3
- 235000011056 potassium acetate Nutrition 0.000 description 3
- 238000012163 sequencing technique Methods 0.000 description 3
- 238000010186 staining Methods 0.000 description 3
- 239000013598 vector Substances 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- MYRTYDVEIRVNKP-UHFFFAOYSA-N 1,2-Divinylbenzene Chemical compound C=CC1=CC=CC=C1C=C MYRTYDVEIRVNKP-UHFFFAOYSA-N 0.000 description 2
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 2
- RGKBRPAAQSHTED-UHFFFAOYSA-N 8-oxoadenine Chemical compound NC1=NC=NC2=C1NC(=O)N2 RGKBRPAAQSHTED-UHFFFAOYSA-N 0.000 description 2
- ZKHQWZAMYRWXGA-KQYNXXCUSA-N Adenosine triphosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-N 0.000 description 2
- ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 239000005089 Luciferase Substances 0.000 description 2
- CHJJGSNFBQVOTG-UHFFFAOYSA-N N-methyl-guanidine Natural products CNC(N)=N CHJJGSNFBQVOTG-UHFFFAOYSA-N 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
- PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical compound C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 description 2
- 229960001456 adenosine triphosphate Drugs 0.000 description 2
- -1 amino- Chemical class 0.000 description 2
- 229960000723 ampicillin Drugs 0.000 description 2
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 238000002659 cell therapy Methods 0.000 description 2
- 230000002759 chromosomal effect Effects 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- SWSQBOPZIKWTGO-UHFFFAOYSA-N dimethylaminoamidine Natural products CN(C)C(N)=N SWSQBOPZIKWTGO-UHFFFAOYSA-N 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 239000002158 endotoxin Substances 0.000 description 2
- 150000002148 esters Chemical class 0.000 description 2
- 239000012894 fetal calf serum Substances 0.000 description 2
- 238000001415 gene therapy Methods 0.000 description 2
- 150000002430 hydrocarbons Chemical group 0.000 description 2
- 238000002743 insertional mutagenesis Methods 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 229910001629 magnesium chloride Inorganic materials 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000006386 neutralization reaction Methods 0.000 description 2
- 239000008194 pharmaceutical composition Substances 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000002560 therapeutic procedure Methods 0.000 description 2
- 229940113082 thymine Drugs 0.000 description 2
- 238000001890 transfection Methods 0.000 description 2
- STGXGJRRAJKJRG-JDJSBBGDSA-N (3r,4r,5r)-5-(hydroxymethyl)-3-methoxyoxolane-2,4-diol Chemical compound CO[C@H]1C(O)O[C@H](CO)[C@H]1O STGXGJRRAJKJRG-JDJSBBGDSA-N 0.000 description 1
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
- MWBWWFOAEOYUST-UHFFFAOYSA-N 2-aminopurine Chemical compound NC1=NC=C2N=CNC2=N1 MWBWWFOAEOYUST-UHFFFAOYSA-N 0.000 description 1
- LQLQRFGHAALLLE-UHFFFAOYSA-N 5-bromouracil Chemical compound BrC1=CNC(=O)NC1=O LQLQRFGHAALLLE-UHFFFAOYSA-N 0.000 description 1
- LRSASMSXMSNRBT-UHFFFAOYSA-N 5-methylcytosine Chemical compound CC1=CNC(=O)N=C1N LRSASMSXMSNRBT-UHFFFAOYSA-N 0.000 description 1
- NXCOIQLTGGBRJE-XLPZGREQSA-N 7-[(2r,4s,5r)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-1h-pyrrolo[2,3-d]pyrimidine-2,4-dione Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(NC(=O)NC2=O)=C2C=C1 NXCOIQLTGGBRJE-XLPZGREQSA-N 0.000 description 1
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- 229930024421 Adenine Natural products 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 1
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 1
- 101100346154 Caenorhabditis elegans oma-1 gene Proteins 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 238000007400 DNA extraction Methods 0.000 description 1
- 230000006820 DNA synthesis Effects 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 108090000204 Dipeptidase 1 Proteins 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 239000004593 Epoxy Substances 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- PEEHTFAAVSWFBL-UHFFFAOYSA-N Maleimide Chemical compound O=C1NC(=O)C=C1 PEEHTFAAVSWFBL-UHFFFAOYSA-N 0.000 description 1
- 241001446467 Mama Species 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 108091005461 Nucleic proteins Proteins 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 108091093037 Peptide nucleic acid Proteins 0.000 description 1
- 108700008625 Reporter Genes Proteins 0.000 description 1
- 108091028664 Ribonucleotide Proteins 0.000 description 1
- 241000831652 Salinivibrio sharmensis Species 0.000 description 1
- 229920005654 Sephadex Polymers 0.000 description 1
- 239000012507 Sephadex™ Substances 0.000 description 1
- 229920002684 Sepharose Polymers 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 description 1
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Natural products O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Natural products O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- 150000001299 aldehydes Chemical group 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 1
- 230000001588 bifunctional effect Effects 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- FUHMZYWBSHTEDZ-UHFFFAOYSA-M bispyribac-sodium Chemical compound [Na+].COC1=CC(OC)=NC(OC=2C(=C(OC=3N=C(OC)C=C(OC)N=3)C=CC=2)C([O-])=O)=N1 FUHMZYWBSHTEDZ-UHFFFAOYSA-M 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 125000002091 cationic group Chemical group 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000006037 cell lysis Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 238000012411 cloning technique Methods 0.000 description 1
- 230000021615 conjugation Effects 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 238000007334 copolymerization reaction Methods 0.000 description 1
- ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 238000010511 deprotection reaction Methods 0.000 description 1
- 238000011026 diafiltration Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 229920006008 lipopolysaccharide Polymers 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- YACKEPLHDIMKIO-UHFFFAOYSA-N methylphosphonic acid Chemical group CP(O)(O)=O YACKEPLHDIMKIO-UHFFFAOYSA-N 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 239000002105 nanoparticle Substances 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- 125000003835 nucleoside group Chemical group 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 230000010412 perfusion Effects 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- PTMHPRAIXMAOOB-UHFFFAOYSA-L phosphoramidate Chemical compound NP([O-])([O-])=O PTMHPRAIXMAOOB-UHFFFAOYSA-L 0.000 description 1
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 210000001236 prokaryotic cell Anatomy 0.000 description 1
- 230000005588 protonation Effects 0.000 description 1
- 229940096913 pseudoisocytidine Drugs 0.000 description 1
- 150000003212 purines Chemical class 0.000 description 1
- 239000012429 reaction media Substances 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 239000002336 ribonucleotide Substances 0.000 description 1
- 125000002652 ribonucleotide group Chemical group 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 235000012239 silicon dioxide Nutrition 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 150000007970 thio esters Chemical class 0.000 description 1
- 125000003396 thiol group Chemical group [H]S* 0.000 description 1
- 150000003573 thiols Chemical class 0.000 description 1
- RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 description 1
- 229940104230 thymidine Drugs 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- 229940035893 uracil Drugs 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6839—Triple helix formation or other higher order conformations in hybridisation assays
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S977/00—Nanotechnology
- Y10S977/902—Specified use of nanostructure
- Y10S977/904—Specified use of nanostructure for medical, immunological, body treatment, or diagnosis
- Y10S977/924—Specified use of nanostructure for medical, immunological, body treatment, or diagnosis using nanostructure as support of dna analysis
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Saccharide Compounds (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR9415162A FR2728264B1 (fr) | 1994-12-16 | 1994-12-16 | Purification d'adn par formation de triple helice avec un oligonucleotide immobilise |
PCT/FR1995/001468 WO1996018744A2 (fr) | 1994-12-16 | 1995-11-08 | Purification d'adn par formation de triple helice avec un oligonucleotide immobilise |
Publications (1)
Publication Number | Publication Date |
---|---|
UA72421C2 true UA72421C2 (en) | 2005-03-15 |
Family
ID=9469865
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
UA97062835A UA72421C2 (en) | 1994-12-16 | 1995-08-11 | A process for dna purifying by formation of triple helix with immobilized oligonucleotides (variants) |
Country Status (31)
Country | Link |
---|---|
US (2) | US6287762B1 (zh) |
EP (2) | EP0797682B1 (zh) |
JP (2) | JPH10510427A (zh) |
KR (1) | KR100431163B1 (zh) |
CN (1) | CN100591776C (zh) |
AT (1) | ATE233825T1 (zh) |
AU (1) | AU715630B2 (zh) |
BG (1) | BG64198B1 (zh) |
BR (1) | BR9510089A (zh) |
CA (1) | CA2208245C (zh) |
CY (1) | CY1110372T1 (zh) |
CZ (1) | CZ295474B6 (zh) |
DE (1) | DE69529842T2 (zh) |
DK (1) | DK0797682T3 (zh) |
ES (2) | ES2197928T3 (zh) |
FI (1) | FI120836B (zh) |
FR (1) | FR2728264B1 (zh) |
HU (1) | HU221062B1 (zh) |
IL (2) | IL171488A (zh) |
MX (1) | MX9703670A (zh) |
NO (1) | NO320428B1 (zh) |
NZ (1) | NZ297023A (zh) |
PL (1) | PL184430B1 (zh) |
PT (1) | PT797682E (zh) |
RO (1) | RO116970B1 (zh) |
RU (1) | RU2174125C2 (zh) |
SK (2) | SK288366B6 (zh) |
TW (1) | TW459043B (zh) |
UA (1) | UA72421C2 (zh) |
WO (1) | WO1996018744A2 (zh) |
ZA (1) | ZA9510756B (zh) |
Families Citing this family (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7038026B2 (en) | 2000-05-26 | 2006-05-02 | Centelion | Purification of a triple heli formation with an immobilized oligonucleotide |
WO1998037231A2 (de) * | 1997-02-22 | 1998-08-27 | Ruprecht-Karls-Universität Heidelberg | Markierung von nukleinsäuren mit speziellen probengemischen |
US20030105040A1 (en) * | 2001-11-13 | 2003-06-05 | Isis Pharmaceuticals Inc. | Antisense modulation of inhibitor-kappa B-R expression |
EP1151093A4 (en) * | 1999-02-04 | 2003-01-08 | Invitrogen Corp | ISOLATION OF NUCLEIC ACID MOLECULES |
US7052844B2 (en) * | 1999-12-21 | 2006-05-30 | Ingeneus, Inc. | Purification of DS-DNA using heteropolymeric capture probes and a triplex, quadruplex or homologous duplex binding mechanism |
AU6345901A (en) | 2000-05-26 | 2001-12-11 | Aventis Pharma Sa | Purification of a triple heli formation with an immobilized oligonucleotide |
US20060008817A1 (en) | 2000-12-08 | 2006-01-12 | Invitrogen Corporation | Methods and compositions for generating recombinant nucleic acid molecules |
DE10104025B4 (de) * | 2001-01-31 | 2008-07-10 | Qiagen North American Holdings, Inc. | Verfahren zur Aufreinigung und anschließenden Amplifikation von Doppelstrang-DNA |
JP4444564B2 (ja) * | 2001-03-23 | 2010-03-31 | サントリオン | 三重螺旋相互作用によるターゲット二本鎖dna配列の精製及び検出方法 |
FR2822476B1 (fr) * | 2001-03-23 | 2004-04-02 | Aventis Pharma Sa | Procedes de purification et de detection de sequences cibles d'adn double brin par interaction triple helice |
KR100790764B1 (ko) | 2002-11-12 | 2008-01-03 | 인디애나 유니버시티 리서치 앤드 테크놀로지 코포레이션 | 조혈세포 집단에서 정지세포를 증대시키는 방법 |
AU2004272748C1 (en) * | 2003-09-17 | 2010-07-08 | Centelion | Method of preparation of pharmaceutically grade plasmid DNA |
DE602005018166D1 (de) | 2004-02-12 | 2010-01-21 | Population Genetics Technologi | Genetische analyse mittels sequenzspezifischem sortieren |
NZ549835A (en) * | 2004-04-19 | 2008-12-24 | Centelion | Method for purifying plasmid DNA |
EP1737945B1 (en) | 2004-04-19 | 2011-01-26 | Aventis Pharma S.A. | Method for purifying plasmid dna |
US8748168B2 (en) | 2004-08-16 | 2014-06-10 | Nature Technology Corp. | Strains of E. coli for plasmid DNA production |
US7407757B2 (en) * | 2005-02-10 | 2008-08-05 | Population Genetics Technologies | Genetic analysis by sequence-specific sorting |
CN105316314B (zh) * | 2014-07-29 | 2018-07-27 | 深圳新诺微环生物科技有限公司 | 一种高纯度的微环dna及其制备方法和应用 |
US10844425B2 (en) | 2017-03-24 | 2020-11-24 | Gen-Probe Incorporated | Compositions and methods for detecting or quantifying parainfluenza virus |
Family Cites Families (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2540122B1 (fr) * | 1983-01-27 | 1985-11-29 | Centre Nat Rech Scient | Nouveaux composes comportant une sequence d'oligonucleotide liee a un agent d'intercalation, leur procede de synthese et leur application |
US5166315A (en) * | 1989-12-20 | 1992-11-24 | Anti-Gene Development Group | Sequence-specific binding polymers for duplex nucleic acids |
US4766072A (en) * | 1985-07-17 | 1988-08-23 | Promega Corporation | Vectors for in vitro production of RNA copies of either strand of a cloned DNA sequence |
US5665541A (en) | 1986-10-28 | 1997-09-09 | The Johns Hopkins University | Formation of triple helix complexes for the detection of double stranded DNA sequences using oligomers which comprise an 8-modified purine base |
WO1989002436A1 (fr) | 1987-09-17 | 1989-03-23 | Appligene | Procede de fixation et de separation de molecules comportant au moins un fragment d'adn naturel ou synthetique |
US5352578A (en) | 1989-02-15 | 1994-10-04 | Worcester Foundation For Experimental Biology | Method of separating oligonucleotides from a mixture |
AU5269590A (en) | 1989-03-10 | 1990-10-09 | Gene-Trak Systems | Immobilized oligonucleotide probes and uses therefor |
CA2015515C (en) * | 1990-01-03 | 1999-12-07 | Jean-Marie Saint-Remy | Pharmaceutical compositions containing antigen-antibody complexes and uses therefor |
AU9094991A (en) * | 1990-11-23 | 1992-06-25 | Gilead Sciences, Inc. | Triplex-forming oligomers containing modified bases |
EP0566670A4 (en) * | 1990-12-17 | 1993-12-08 | Idexx Laboratories, Inc. | Nucleic acid sequence detection by triple helix formation |
WO1992013963A1 (en) | 1991-01-30 | 1992-08-20 | Hyman Edward D | Method for preparation of closed circular dna |
EP0533952A4 (en) | 1991-04-15 | 1993-07-07 | Sumitomo Electric Industries, Ltd. | Process for separating or extracting nucleic acid from specimen containing the same |
RU2186852C2 (ru) | 1991-12-24 | 2002-08-10 | Тепнел Медикал Лимитед | Способ выполнения манипуляции с последовательностью нуклеиновой кислоты, устройство для проведения манипуляций на последовательности нуклеиновой кислоты, проточный сосуд, твердый носитель для иммобилизации последовательности нуклеиновой кислоты, твердый носитель, способ получения носителя для иммобилизации последовательности нуклеиновой кислоты |
AU4544193A (en) | 1992-06-25 | 1994-01-24 | Microprobe Corporation | Solid supports for nucleic acid hybridization assays |
US5401632A (en) | 1992-07-16 | 1995-03-28 | Wisconsin Alumni Research Foundation | Triple helix purification and sequencing |
WO1994017086A1 (en) * | 1993-01-25 | 1994-08-04 | Apollon, Inc. | Gene regulation by targeting putative intramolecular triple helix |
-
1994
- 1994-12-16 FR FR9415162A patent/FR2728264B1/fr not_active Expired - Lifetime
-
1995
- 1995-08-11 UA UA97062835A patent/UA72421C2/uk unknown
- 1995-11-08 CZ CZ19971858A patent/CZ295474B6/cs not_active IP Right Cessation
- 1995-11-08 EP EP95940285A patent/EP0797682B1/fr not_active Expired - Lifetime
- 1995-11-08 CA CA2208245A patent/CA2208245C/fr not_active Expired - Lifetime
- 1995-11-08 DE DE69529842T patent/DE69529842T2/de not_active Expired - Lifetime
- 1995-11-08 EP EP02012081.2A patent/EP1281774B1/fr not_active Expired - Lifetime
- 1995-11-08 SK SK5104-2008A patent/SK288366B6/sk not_active IP Right Cessation
- 1995-11-08 US US08/860,038 patent/US6287762B1/en not_active Expired - Lifetime
- 1995-11-08 SK SK756-97A patent/SK286956B6/sk not_active IP Right Cessation
- 1995-11-08 DK DK95940285T patent/DK0797682T3/da active
- 1995-11-08 PT PT95940285T patent/PT797682E/pt unknown
- 1995-11-08 HU HU9701886A patent/HU221062B1/hu unknown
- 1995-11-08 NZ NZ297023A patent/NZ297023A/xx not_active IP Right Cessation
- 1995-11-08 KR KR1019970703997A patent/KR100431163B1/ko not_active IP Right Cessation
- 1995-11-08 CN CN95196817A patent/CN100591776C/zh not_active Expired - Lifetime
- 1995-11-08 JP JP8518319A patent/JPH10510427A/ja active Pending
- 1995-11-08 MX MX9703670A patent/MX9703670A/es unknown
- 1995-11-08 RU RU97111878/1497111878/14A patent/RU2174125C2/ru active
- 1995-11-08 AT AT95940285T patent/ATE233825T1/de active
- 1995-11-08 BR BR9510089A patent/BR9510089A/pt not_active Application Discontinuation
- 1995-11-08 RO RO97-01102A patent/RO116970B1/ro unknown
- 1995-11-08 ES ES95940285T patent/ES2197928T3/es not_active Expired - Lifetime
- 1995-11-08 PL PL95320697A patent/PL184430B1/pl unknown
- 1995-11-08 WO PCT/FR1995/001468 patent/WO1996018744A2/fr active Application Filing
- 1995-11-08 AU AU41789/96A patent/AU715630B2/en not_active Expired
- 1995-11-08 ES ES02012081.2T patent/ES2446983T3/es not_active Expired - Lifetime
- 1995-12-14 IL IL171488A patent/IL171488A/en not_active IP Right Cessation
- 1995-12-14 IL IL116398A patent/IL116398A/en not_active IP Right Cessation
- 1995-12-16 TW TW084113451A patent/TW459043B/zh not_active IP Right Cessation
- 1995-12-18 ZA ZA9510756A patent/ZA9510756B/xx unknown
-
1997
- 1997-06-12 NO NO19972710A patent/NO320428B1/no not_active IP Right Cessation
- 1997-06-13 FI FI972526A patent/FI120836B/fi not_active IP Right Cessation
- 1997-06-16 BG BG101620A patent/BG64198B1/bg unknown
-
2000
- 2000-05-26 US US09/580,923 patent/US6319672B1/en not_active Expired - Lifetime
-
2008
- 2008-03-07 CY CY20081100271T patent/CY1110372T1/el unknown
- 2008-10-16 JP JP2008267538A patent/JP2009045070A/ja active Pending
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
UA72421C2 (en) | A process for dna purifying by formation of triple helix with immobilized oligonucleotides (variants) | |
US6022691A (en) | Determination of oligonucleotides for therapeutics, diagnostics and research reagents | |
US8399636B2 (en) | Purification of a triple helix formation with an immobilized obligonucleotide | |
US5478731A (en) | Polycos vectors | |
AU2007202804B8 (en) | Purification of a triple helix formation with an immobilized oligonucleotide | |
NZ528507A (en) | Processes for purifying and for detecting target double-stranded DNA sequences by triple helix interaction | |
Giacomoni | The origin of DNA: RNA hybridization | |
US7514218B2 (en) | Processes for purifying and for detecting target double-stranded DNA sequences by triple helix interaction | |
AU2001263459A1 (en) | Purification of a triple heli formation with an immobilized oligonucleotide | |
IL152860A (en) | Purification of dna by a triple helix formation with an immobilized oligonucleotide |