UA40648C2 - Днк експресуюча касета, дріжджовий вектор експресії, спосіб одержання поліпептиду - Google Patents
Днк експресуюча касета, дріжджовий вектор експресії, спосіб одержання поліпептидуInfo
- Publication number
- UA40648C2 UA40648C2 UA96124661A UA96124661A UA40648C2 UA 40648 C2 UA40648 C2 UA 40648C2 UA 96124661 A UA96124661 A UA 96124661A UA 96124661 A UA96124661 A UA 96124661A UA 40648 C2 UA40648 C2 UA 40648C2
- Authority
- UA
- Ukraine
- Prior art keywords
- coding
- sequence
- glu
- asp
- amino acid
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Mycology (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Endocrinology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Diabetes (AREA)
- Medicinal Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
Винахід належить до синтетичних лідерних пептидних послідовностей для секреції поліпептидів в дріжджах. ДНК експресуюча касета, причому включає послідовність 5'-P-SP-LS-PS-“ген”-(T)i-3', де З означає промоторну послідовність; SP означає ДНК послідовність, що кодує сигнальний пептид; LS означає ДНК послідовність, що кодує лідерний пептид загальної формули I: GlnProIle(Asp/Glu) (Asp/Glu)X1(Glu/Asp)X2Asnz(Thr/Ser)X3 , (I), де X1 означає пептидний зв'язок або кодовану амінокислоту, X2 означає пептидний зв'язок або кодовану амінокислоту, або послідовність до 4 однакових або різних кодованих амінокислот; Ζ означає кодовану амінокислоту окрім Pro, і X3 означає послідовність від 4 до 30 однакових або різних кодованих амінокислот; PS означає ДНК послідовність, що кодує процесинговий сайт; “ген” означає ДНК послідовність, що кодує поліпептид; Т означає термінаторну послідовність, і і дорівнює 0 або 1.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DK70594 | 1994-06-16 | ||
US28285294A | 1994-07-29 | 1994-07-29 | |
PCT/DK1995/000249 WO1995034666A1 (en) | 1994-06-16 | 1995-06-16 | Synthetic leader peptide sequences |
Publications (1)
Publication Number | Publication Date |
---|---|
UA40648C2 true UA40648C2 (uk) | 2001-08-15 |
Family
ID=26064490
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
UA96124661A UA40648C2 (uk) | 1994-06-16 | 1995-06-16 | Днк експресуюча касета, дріжджовий вектор експресії, спосіб одержання поліпептиду |
Country Status (20)
Country | Link |
---|---|
US (2) | US5639642A (uk) |
EP (1) | EP0763117B1 (uk) |
JP (1) | JP3676369B2 (uk) |
KR (1) | KR100251054B1 (uk) |
CN (1) | CN1115413C (uk) |
AT (1) | ATE208823T1 (uk) |
AU (1) | AU693569B2 (uk) |
BR (1) | BR9508033A (uk) |
CZ (1) | CZ290069B6 (uk) |
DE (1) | DE69523915T2 (uk) |
DK (1) | DK0763117T3 (uk) |
ES (1) | ES2168367T3 (uk) |
FI (1) | FI965005A (uk) |
HU (1) | HU221118B1 (uk) |
MX (1) | MX9606492A (uk) |
NO (1) | NO965362L (uk) |
PL (1) | PL317722A1 (uk) |
PT (1) | PT763117E (uk) |
UA (1) | UA40648C2 (uk) |
WO (1) | WO1995034666A1 (uk) |
Families Citing this family (48)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2686899B1 (fr) * | 1992-01-31 | 1995-09-01 | Rhone Poulenc Rorer Sa | Nouveaux polypeptides biologiquement actifs, leur preparation et compositions pharmaceutiques les contenant. |
US6500645B1 (en) | 1994-06-17 | 2002-12-31 | Novo Nordisk A/S | N-terminally extended proteins expressed in yeast |
ZA9610456B (en) * | 1995-12-20 | 1997-06-20 | Novo Nordisk As | N-terminally extended proteins expressed in yeast |
EP0946736B1 (en) * | 1996-12-13 | 2003-10-29 | Chiron Corporation | Method for expression of heterologous proteins in yeast |
DE69735242T2 (de) * | 1996-12-20 | 2006-09-21 | Novo Nordisk A/S | N-terminal verlängerte proteine exprimiert in hefe |
JP2001527387A (ja) * | 1997-01-24 | 2001-12-25 | ノボ ノルディスク アクティーゼルスカブ | 合成リーダーペプチド配列 |
CA2205076A1 (en) | 1997-05-14 | 1998-11-14 | Jim Hu | Episomal expression cassettes for gene therapy |
KR20010034305A (ko) | 1998-01-23 | 2001-04-25 | 한센 핀 베네드 | 효모에서 원하는 폴리펩티드를 만드는 방법 |
AU1995701A (en) | 1999-12-29 | 2001-07-16 | Novo Nordisk A/S | Method for making insulin precursors and insulin precursor analogues having improved fermentation yield in yeast |
WO2001079480A1 (en) * | 2000-04-12 | 2001-10-25 | Human Genome Sciences, Inc. | Albumin fusion proteins |
US20050100991A1 (en) * | 2001-04-12 | 2005-05-12 | Human Genome Sciences, Inc. | Albumin fusion proteins |
US20030143191A1 (en) * | 2001-05-25 | 2003-07-31 | Adam Bell | Chemokine beta-1 fusion proteins |
WO2003059934A2 (en) * | 2001-12-21 | 2003-07-24 | Human Genome Sciences, Inc. | Albumin fusion proteins |
WO2005077042A2 (en) * | 2004-02-09 | 2005-08-25 | Human Genome Sciences, Inc. | Albumin fusion proteins |
EP1997829A1 (en) * | 2001-12-21 | 2008-12-03 | Human Genome Sciences, Inc. | Albumin fusion proteins |
GB0217033D0 (en) | 2002-07-23 | 2002-08-28 | Delta Biotechnology Ltd | Gene and polypeptide sequences |
EP1594530A4 (en) | 2003-01-22 | 2006-10-11 | Human Genome Sciences Inc | HYBRID PROTEINS OF ALBUMIN |
JP2007532096A (ja) | 2003-11-14 | 2007-11-15 | ノボ ノルディスク アクティーゼルスカブ | アシル化されたインスリンの製造方法 |
ES2369895T3 (es) | 2003-12-03 | 2011-12-07 | Novo Nordisk A/S | Insulina monocatenaria. |
US7527947B2 (en) | 2004-06-14 | 2009-05-05 | Novozymes A/S | Signal peptide for producing a polypeptide |
JP5366546B2 (ja) | 2005-08-16 | 2013-12-11 | ノボ・ノルデイスク・エー/エス | 成熟インスリンポリペプチドの作製方法 |
US20090074733A1 (en) * | 2005-12-09 | 2009-03-19 | Medin Jeffrey A | Thymidylate kinase mutants and uses thereof |
US20090068158A1 (en) * | 2005-12-09 | 2009-03-12 | Medin Jeffrey A | Thymidylate kinase mutants and uses thereof |
ATE557037T1 (de) | 2006-02-27 | 2012-05-15 | Novo Nordisk As | Insulin derivate |
CA2642848C (en) | 2006-03-03 | 2016-07-12 | Amorfix Life Sciences Ltd. | Methods and compositions to treat and detect misfolded-sod1 mediated diseases |
BRPI0717098B8 (pt) | 2006-09-22 | 2021-05-25 | Novo Nordisk As | análogos de insulina humana resistentes à protease, composição farmacêutica e processo para sua preparação |
ES2464282T3 (es) | 2006-09-27 | 2014-06-02 | Novo Nordisk A/S | Método para preparar polipéptidos de insulina madura |
CA2584494A1 (en) * | 2007-03-27 | 2008-09-27 | Jeffrey A. Medin | Vector encoding therapeutic polypeptide and safety elements to clear transduced cells |
WO2008134879A1 (en) | 2007-05-04 | 2008-11-13 | University Health Network | Il-12 immunotherapy for cancer |
WO2008139496A1 (en) * | 2007-05-16 | 2008-11-20 | Bigtec Private Limited | Recombinant human insulin and a method thereof |
JP5688969B2 (ja) | 2007-07-16 | 2015-03-25 | ノボ・ノルデイスク・エー/エス | プロテアーゼに対して安定しているペグ化インスリンアナログ |
MX2010009850A (es) | 2008-03-18 | 2010-09-30 | Novo Nordisk As | Analogos de insulina acilados y etabilizados contra proteasas. |
US8568709B2 (en) * | 2008-03-20 | 2013-10-29 | University Health Network | Thymidylate kinase fusions and uses thereof |
JP5850848B2 (ja) | 2009-11-25 | 2016-02-03 | ノヴォ ノルディスク アー/エス | ポリペプチドの製造方法 |
ES2543634T3 (es) | 2010-01-22 | 2015-08-20 | Novo Nordisk A/S | Proceso para preparación de FGF-21 con grado de O-glicosilación bajo |
RU2460795C1 (ru) * | 2011-07-06 | 2012-09-10 | Федеральное государственное унитарное предприятие "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов" (ФГУП "ГосНИИгенетика") | Способ микробиологического синтеза секретируемого соматотропина человека и штамм дрожжей saccharomyces cerevisiae - продуцент секретируемого соматотропина человека |
EP2920319B1 (en) | 2012-11-16 | 2020-02-19 | Poseida Therapeutics, Inc. | Site-specific enzymes and methods of use |
CA2890048C (en) | 2012-12-03 | 2022-05-03 | Merck Sharp & Dohme Corp. | O-glycosylated carboxy terminal portion (ctp) peptide-based insulin and insulin analogues |
EP3004156A1 (en) | 2013-06-07 | 2016-04-13 | Novo Nordisk A/S | Method for making mature insulin polypeptides |
WO2015128507A1 (en) | 2014-02-28 | 2015-09-03 | Novo Nordisk A/S | Mating factor alpha pro-peptide variants |
US20170151281A1 (en) | 2015-02-19 | 2017-06-01 | Batu Biologics, Inc. | Chimeric antigen receptor dendritic cell (car-dc) for treatment of cancer |
US10562951B2 (en) | 2015-03-10 | 2020-02-18 | Merck Sharp & Dohme Corp. | Process for preparing recombinant insulin using microfiltration |
EP3929286A1 (en) | 2015-06-17 | 2021-12-29 | Poseida Therapeutics, Inc. | Compositions and methods for directing proteins to specific loci in the genome |
CN105418755A (zh) * | 2015-12-28 | 2016-03-23 | 珠海冀百康生物科技有限公司 | 速效胰岛素前体蛋白以及速效胰岛素的制备方法 |
WO2019126578A1 (en) | 2017-12-20 | 2019-06-27 | Poseida Therapeutics, Inc. | Compositions and methods for directing proteins to specific loci in the genome |
US20210032307A1 (en) * | 2018-02-09 | 2021-02-04 | Jiangsu Hengrui Medicine Co., Ltd. | Codon optimized precursor gene and signal peptide gene of human insulin analogue |
KR20230133837A (ko) | 2020-11-04 | 2023-09-19 | 마이얼로이드 테라퓨틱스, 인크. | 조작된 키메라 융합 단백질 조성물 및 그의 사용 방법 |
EP4308133A2 (en) | 2021-03-17 | 2024-01-24 | Myeloid Therapeutics, Inc. | Engineered chimeric fusion protein compositions and methods of use thereof |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA1340772C (en) * | 1987-12-30 | 1999-09-28 | Patricia Tekamp-Olson | Expression and secretion of heterologous protiens in yeast employing truncated alpha-factor leader sequences |
US5037743A (en) * | 1988-08-05 | 1991-08-06 | Zymogenetics, Inc. | BAR1 secretion signal |
DK300090D0 (da) * | 1990-12-19 | 1990-12-19 | Novo Nordisk As | Fremgangsmaade til fremstilling af leadersekvenser |
-
1995
- 1995-06-06 US US08/468,674 patent/US5639642A/en not_active Expired - Lifetime
- 1995-06-16 HU HU9603477A patent/HU221118B1/hu not_active IP Right Cessation
- 1995-06-16 CN CN95194183A patent/CN1115413C/zh not_active Expired - Lifetime
- 1995-06-16 CZ CZ19963641A patent/CZ290069B6/cs not_active IP Right Cessation
- 1995-06-16 PL PL95317722A patent/PL317722A1/xx unknown
- 1995-06-16 BR BR9508033A patent/BR9508033A/pt not_active IP Right Cessation
- 1995-06-16 AT AT95922439T patent/ATE208823T1/de not_active IP Right Cessation
- 1995-06-16 AU AU27334/95A patent/AU693569B2/en not_active Ceased
- 1995-06-16 KR KR1019960707199A patent/KR100251054B1/ko not_active IP Right Cessation
- 1995-06-16 DE DE69523915T patent/DE69523915T2/de not_active Expired - Lifetime
- 1995-06-16 PT PT95922439T patent/PT763117E/pt unknown
- 1995-06-16 UA UA96124661A patent/UA40648C2/uk unknown
- 1995-06-16 WO PCT/DK1995/000249 patent/WO1995034666A1/en active IP Right Grant
- 1995-06-16 JP JP50150496A patent/JP3676369B2/ja not_active Expired - Lifetime
- 1995-06-16 ES ES95922439T patent/ES2168367T3/es not_active Expired - Lifetime
- 1995-06-16 DK DK95922439T patent/DK0763117T3/da active
- 1995-06-16 MX MX9606492A patent/MX9606492A/es unknown
- 1995-06-16 EP EP95922439A patent/EP0763117B1/en not_active Expired - Lifetime
-
1996
- 1996-12-13 FI FI965005A patent/FI965005A/fi unknown
- 1996-12-13 NO NO965362A patent/NO965362L/no unknown
-
1997
- 1997-01-08 US US08/780,571 patent/US5795746A/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
NO965362D0 (no) | 1996-12-13 |
EP0763117A1 (en) | 1997-03-19 |
JPH10501413A (ja) | 1998-02-10 |
KR100251054B1 (ko) | 2000-04-15 |
HUT76378A (en) | 1997-08-28 |
CN1154143A (zh) | 1997-07-09 |
US5639642A (en) | 1997-06-17 |
MX9606492A (es) | 1997-03-29 |
US5795746A (en) | 1998-08-18 |
ATE208823T1 (de) | 2001-11-15 |
HU221118B1 (en) | 2002-08-28 |
AU693569B2 (en) | 1998-07-02 |
PT763117E (pt) | 2002-05-31 |
WO1995034666A1 (en) | 1995-12-21 |
JP3676369B2 (ja) | 2005-07-27 |
CZ290069B6 (cs) | 2002-05-15 |
DE69523915D1 (en) | 2001-12-20 |
CN1115413C (zh) | 2003-07-23 |
HU9603477D0 (en) | 1997-02-28 |
ES2168367T3 (es) | 2002-06-16 |
FI965005A0 (fi) | 1996-12-13 |
EP0763117B1 (en) | 2001-11-14 |
BR9508033A (pt) | 1999-06-29 |
NO965362L (no) | 1997-02-13 |
AU2733495A (en) | 1996-01-05 |
DK0763117T3 (da) | 2002-02-18 |
FI965005A (fi) | 1997-02-13 |
CZ364196A3 (en) | 1997-05-14 |
DE69523915T2 (de) | 2002-08-22 |
PL317722A1 (en) | 1997-04-28 |
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