ES2008391A6 - Un metodo para producir isopenicilina n sintetasa. - Google Patents

Un metodo para producir isopenicilina n sintetasa.

Info

Publication number
ES2008391A6
ES2008391A6 ES8703592A ES8703592A ES2008391A6 ES 2008391 A6 ES2008391 A6 ES 2008391A6 ES 8703592 A ES8703592 A ES 8703592A ES 8703592 A ES8703592 A ES 8703592A ES 2008391 A6 ES2008391 A6 ES 2008391A6
Authority
ES
Spain
Prior art keywords
synthetase
isopenicillin
nidulans
encoding
dna encoding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES8703592A
Other languages
English (en)
Inventor
Daniel Ramon Vidal
Soto Miguel Angel Penalba
Ortega Agustin Perez-Aranda
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Antibioticos SA
Original Assignee
Antibioticos SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Antibioticos SA filed Critical Antibioticos SA
Publication of ES2008391A6 publication Critical patent/ES2008391A6/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

UN METODO PARA PRODUCIR ISOPENICILINA N SINTETASA. COMPRENDE: A) DIGERIR EL ADN DE UN FAGO RECOMBINANTE CON UN INSERTO DE ADN DE A. NIDULANS Y AISLAR UN FRAGMENTO DE ADN; B) INCUBAR EL MISMO CON PUC13 CON UNA ADN LIGASA; C) TRANSFORMAR UNA CELULA HUESPED CON LA MEZCLA Y AISLAR UN ADN PLASMIDICO CIRCULAR; D) DIGERIR PCIANH2 PRODUCIENDOSE UN FRAGMENTO DE ADN QUE INCLUYE PARTE DE LA SECUENCIA DE BASES DEL GEN DE LA ISOPENICILINA N SINTETASA; E) REANILLAR EL ADN CON OLIGONUCLEOTIDOS SINTETICOS COMPLEMENTARIOS OBTENIENDOSE UN ADAPTADOR QUE RECONSTRUYE LA SECUENCIA DE NUCLEOTIDOS NECESARIA; F) DIGERIR PIM-IIIAL Y MEZCLAR LOS FRAGMENTOS CON ADN LIGASA TRANSFORMANDO CELULAS CON LA MEZCLA, OBTENIENDOSE PIM-CIC; Y G) TRANSFORMAR CELULAS CON EL MISMO AISLANDOSE FINALMENTE ISOPENICILINA N SINTETASA. LA ISOPENICILINA N SINTETASA TIENE APLICACION EN LA BIOSINTESIS DE ANTIBIOTICOS LACTAMICOS.
ES8703592A 1987-04-24 1987-12-15 Un metodo para producir isopenicilina n sintetasa. Expired ES2008391A6 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB8709708A GB2208077A (en) 1987-04-24 1987-04-24 Isopenicillin n synthetase gene derived from aspergillus nidulans

Publications (1)

Publication Number Publication Date
ES2008391A6 true ES2008391A6 (es) 1989-07-16

Family

ID=10616271

Family Applications (1)

Application Number Title Priority Date Filing Date
ES8703592A Expired ES2008391A6 (es) 1987-04-24 1987-12-15 Un metodo para producir isopenicilina n sintetasa.

Country Status (5)

Country Link
EP (1) EP0288325B1 (es)
AT (1) ATE107694T1 (es)
DE (1) DE3850294T2 (es)
ES (1) ES2008391A6 (es)
GB (1) GB2208077A (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6258555B1 (en) 1987-12-09 2001-07-10 Beecham Group P.L.C. DNA encoding ACV synthetase
GB8728811D0 (en) * 1987-12-09 1988-01-27 Beecham Group Plc Novel substance

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL78531A0 (en) * 1985-04-22 1986-08-31 Lilly Co Eli Recombinant dna expression vectors and dna compounds which encode isopenicillin n synthetase
US4892819A (en) * 1985-11-25 1990-01-09 Eli Lilly And Company Recombinant DNA expression vectors and DNA compounds that encode isopenicillin N synthetase from penicillium chrysogenum

Also Published As

Publication number Publication date
GB2208077A (en) 1989-02-22
EP0288325A3 (en) 1989-04-26
DE3850294T2 (de) 1995-01-19
EP0288325A2 (en) 1988-10-26
ATE107694T1 (de) 1994-07-15
EP0288325B1 (en) 1994-06-22
DE3850294D1 (de) 1994-07-28
GB8709708D0 (en) 1987-05-28

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Legal Events

Date Code Title Description
SA6 Expiration date (snapshot 920101)

Free format text: 2007-12-15