BR9508033A - Cassete de expressão de dna vetor de expressão de levedura célula de levedura processo para produzir um polipeptídeo em levedura - Google Patents

Cassete de expressão de dna vetor de expressão de levedura célula de levedura processo para produzir um polipeptídeo em levedura

Info

Publication number
BR9508033A
BR9508033A BR9508033A BR9508033A BR9508033A BR 9508033 A BR9508033 A BR 9508033A BR 9508033 A BR9508033 A BR 9508033A BR 9508033 A BR9508033 A BR 9508033A BR 9508033 A BR9508033 A BR 9508033A
Authority
BR
Brazil
Prior art keywords
yeast
polypeptide
dna
codable amino
produce
Prior art date
Application number
BR9508033A
Other languages
English (en)
Inventor
Thomas Borglum Kjeldsen
Knud Vad
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Publication of BR9508033A publication Critical patent/BR9508033A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Diabetes (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
BR9508033A 1994-06-16 1995-06-16 Cassete de expressão de dna vetor de expressão de levedura célula de levedura processo para produzir um polipeptídeo em levedura BR9508033A (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DK70594 1994-06-16
US28285294A 1994-07-29 1994-07-29
PCT/DK1995/000249 WO1995034666A1 (en) 1994-06-16 1995-06-16 Synthetic leader peptide sequences

Publications (1)

Publication Number Publication Date
BR9508033A true BR9508033A (pt) 1999-06-29

Family

ID=26064490

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9508033A BR9508033A (pt) 1994-06-16 1995-06-16 Cassete de expressão de dna vetor de expressão de levedura célula de levedura processo para produzir um polipeptídeo em levedura

Country Status (20)

Country Link
US (2) US5639642A (pt)
EP (1) EP0763117B1 (pt)
JP (1) JP3676369B2 (pt)
KR (1) KR100251054B1 (pt)
CN (1) CN1115413C (pt)
AT (1) ATE208823T1 (pt)
AU (1) AU693569B2 (pt)
BR (1) BR9508033A (pt)
CZ (1) CZ290069B6 (pt)
DE (1) DE69523915T2 (pt)
DK (1) DK0763117T3 (pt)
ES (1) ES2168367T3 (pt)
FI (1) FI965005A (pt)
HU (1) HU221118B1 (pt)
MX (1) MX9606492A (pt)
NO (1) NO965362L (pt)
PL (1) PL317722A1 (pt)
PT (1) PT763117E (pt)
UA (1) UA40648C2 (pt)
WO (1) WO1995034666A1 (pt)

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FR2686899B1 (fr) * 1992-01-31 1995-09-01 Rhone Poulenc Rorer Sa Nouveaux polypeptides biologiquement actifs, leur preparation et compositions pharmaceutiques les contenant.
US6500645B1 (en) 1994-06-17 2002-12-31 Novo Nordisk A/S N-terminally extended proteins expressed in yeast
ZA9610456B (en) * 1995-12-20 1997-06-20 Novo Nordisk As N-terminally extended proteins expressed in yeast
WO1998026080A1 (en) * 1996-12-13 1998-06-18 Chiron Corporation Method for expression of heterologous proteins in yeast
ES2258797T3 (es) * 1996-12-20 2006-09-01 Novo Nordisk A/S Proteinas con extension n-terminal expresadas en la levadura.
AU5549998A (en) 1997-01-24 1998-08-18 Novo Nordisk A/S Synthetic leader peptide sequences
CA2205076A1 (en) 1997-05-14 1998-11-14 Jim Hu Episomal expression cassettes for gene therapy
AU2149899A (en) 1998-01-23 1999-08-09 Novo Nordisk A/S Process for making desired polypeptides in yeast
WO2001049870A1 (en) 1999-12-29 2001-07-12 Novo Nordisk A/S Method for making insulin precursors and insulin precursor analogues having improved fermentation yield in yeast
US20050100991A1 (en) * 2001-04-12 2005-05-12 Human Genome Sciences, Inc. Albumin fusion proteins
EP1278544A4 (en) * 2000-04-12 2004-08-18 Human Genome Sciences Inc ALBUMIN FUSION PROTEINS
CA2446739A1 (en) * 2001-05-25 2002-12-05 Human Genome Sciences, Inc. Chemokine beta-1 fusion proteins
WO2005003296A2 (en) 2003-01-22 2005-01-13 Human Genome Sciences, Inc. Albumin fusion proteins
AU2002364587A1 (en) * 2001-12-21 2003-07-30 Human Genome Sciences, Inc. Albumin fusion proteins
WO2003060071A2 (en) 2001-12-21 2003-07-24 Human Genome Sciences, Inc. Albumin fusion proteins
GB0217033D0 (en) * 2002-07-23 2002-08-28 Delta Biotechnology Ltd Gene and polypeptide sequences
WO2005047508A1 (en) 2003-11-14 2005-05-26 Novo Nordisk A/S Processes for making acylated insulin
EP1692168B1 (en) 2003-12-03 2011-07-20 Novo Nordisk A/S Single-chain insulin
KR101184391B1 (ko) * 2004-02-09 2013-03-14 휴먼 게놈 사이언시즈, 인코포레이티드 알부민 융합 단백질
US7527947B2 (en) * 2004-06-14 2009-05-05 Novozymes A/S Signal peptide for producing a polypeptide
US9056921B2 (en) 2005-08-16 2015-06-16 Novo Nordisk A/S Method for making mature insulin polypeptides
CA2566267A1 (en) * 2005-12-09 2007-06-09 University Health Network Thymidylate kinase mutants and uses thereof
US20090068158A1 (en) * 2005-12-09 2009-03-12 Medin Jeffrey A Thymidylate kinase mutants and uses thereof
DE602007009496D1 (de) 2006-02-27 2010-11-11 Novo Nordisk As Insulinderivate
AU2007219615B2 (en) 2006-03-03 2013-11-28 Promis Neurosciences Inc. Methods and compositions to treat and detect misfolded-SOD1 mediated diseases
WO2008034881A1 (en) 2006-09-22 2008-03-27 Novo Nordisk A/S Protease resistant insulin analogues
ES2464282T3 (es) 2006-09-27 2014-06-02 Novo Nordisk A/S Método para preparar polipéptidos de insulina madura
CA2584494A1 (en) * 2007-03-27 2008-09-27 Jeffrey A. Medin Vector encoding therapeutic polypeptide and safety elements to clear transduced cells
ES2654303T3 (es) 2007-05-04 2018-02-13 University Health Network Inmunoterapia de IL-12 contra el cáncer
WO2008139496A1 (en) * 2007-05-16 2008-11-20 Bigtec Private Limited Recombinant human insulin and a method thereof
JP5688969B2 (ja) 2007-07-16 2015-03-25 ノボ・ノルデイスク・エー／エス プロテアーゼに対して安定しているペグ化インスリンアナログ
WO2009115469A1 (en) 2008-03-18 2009-09-24 Novo Nordisk A/S Protease stabilized, acylated insulin analogues
US8568709B2 (en) * 2008-03-20 2013-10-29 University Health Network Thymidylate kinase fusions and uses thereof
WO2011064282A1 (en) 2009-11-25 2011-06-03 Novo Nordisk A/S Method for making polypeptides
CN102791730A (zh) 2010-01-22 2012-11-21 诺沃—诺迪斯克有限公司 低度o-糖基化的fgf21的制备方法
RU2460795C1 (ru) * 2011-07-06 2012-09-10 Федеральное государственное унитарное предприятие "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов" (ФГУП "ГосНИИгенетика") Способ микробиологического синтеза секретируемого соматотропина человека и штамм дрожжей saccharomyces cerevisiae - продуцент секретируемого соматотропина человека
EP2920319B1 (en) 2012-11-16 2020-02-19 Poseida Therapeutics, Inc. Site-specific enzymes and methods of use
WO2014088836A1 (en) 2012-12-03 2014-06-12 Merck Sharp & Dohme Corp. O-glycosylated carboxy terminal portion (ctp) peptide-based insulin and insulin analogues
CN105308067B (zh) 2013-06-07 2020-07-24 诺和诺德股份有限公司 制备成熟胰岛素多肽的方法
JP6275847B2 (ja) 2014-02-28 2018-02-07 ノヴォ ノルディスク アー／エス アルファ接合因子プロペプチドバリアント
US20170151281A1 (en) 2015-02-19 2017-06-01 Batu Biologics, Inc. Chimeric antigen receptor dendritic cell (car-dc) for treatment of cancer
US10562951B2 (en) 2015-03-10 2020-02-18 Merck Sharp & Dohme Corp. Process for preparing recombinant insulin using microfiltration
ES2886599T3 (es) 2015-06-17 2021-12-20 Poseida Therapeutics Inc Composiciones y métodos para dirigir proteínas a loci específicos en el genoma
CN105418755A (zh) * 2015-12-28 2016-03-23 珠海冀百康生物科技有限公司 速效胰岛素前体蛋白以及速效胰岛素的制备方法
WO2019126578A1 (en) 2017-12-20 2019-06-27 Poseida Therapeutics, Inc. Compositions and methods for directing proteins to specific loci in the genome
US20210032307A1 (en) * 2018-02-09 2021-02-04 Jiangsu Hengrui Medicine Co., Ltd. Codon optimized precursor gene and signal peptide gene of human insulin analogue
CA3197423A1 (en) 2020-11-04 2022-05-12 Daniel Getts Engineered chimeric fusion protein compositions and methods of use thereof
MX2023010916A (es) 2021-03-17 2023-12-14 Myeloid Therapeutics Inc Composiciones de proteinas de fusion quimericas modificadas por ingenieria y metodos de uso de las mismas.

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CA1340772C (en) * 1987-12-30 1999-09-28 Patricia Tekamp-Olson Expression and secretion of heterologous protiens in yeast employing truncated alpha-factor leader sequences
US5037743A (en) * 1988-08-05 1991-08-06 Zymogenetics, Inc. BAR1 secretion signal
DK300090D0 (da) * 1990-12-19 1990-12-19 Novo Nordisk As Fremgangsmaade til fremstilling af leadersekvenser

Also Published As

Publication number Publication date
PL317722A1 (en) 1997-04-28
ES2168367T3 (es) 2002-06-16
DE69523915T2 (de) 2002-08-22
HU221118B1 (en) 2002-08-28
CZ364196A3 (en) 1997-05-14
EP0763117B1 (en) 2001-11-14
MX9606492A (es) 1997-03-29
CN1154143A (zh) 1997-07-09
AU2733495A (en) 1996-01-05
HUT76378A (en) 1997-08-28
PT763117E (pt) 2002-05-31
NO965362D0 (no) 1996-12-13
CN1115413C (zh) 2003-07-23
NO965362L (no) 1997-02-13
DE69523915D1 (en) 2001-12-20
HU9603477D0 (en) 1997-02-28
ATE208823T1 (de) 2001-11-15
US5795746A (en) 1998-08-18
FI965005A0 (fi) 1996-12-13
CZ290069B6 (cs) 2002-05-15
UA40648C2 (uk) 2001-08-15
US5639642A (en) 1997-06-17
EP0763117A1 (en) 1997-03-19
JPH10501413A (ja) 1998-02-10
KR100251054B1 (ko) 2000-04-15
DK0763117T3 (da) 2002-02-18
FI965005A (fi) 1997-02-13
JP3676369B2 (ja) 2005-07-27
WO1995034666A1 (en) 1995-12-21
AU693569B2 (en) 1998-07-02

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Legal Events

Date Code Title Description
FA10 Dismissal: dismissal - article 33 of industrial property law
HKFN Application deemed withdrawn (deleted)
FB36 Technical and formal requirements: requirement - article 36 of industrial property law
FF Decision: intention to grant
FG9A Patent or certificate of addition granted
B21A Patent or certificate of addition expired [chapter 21.1 patent gazette]

Free format text: PATENTE EXTINTA EM 16/06/2015