TWI698520B - 用於從微生物細胞獲得微生物油之方法（三） - Google Patents

用於從微生物細胞獲得微生物油之方法（三） Download PDF

Info

Publication number
TWI698520B
TWI698520B TW103144476A TW103144476A TWI698520B TW I698520 B TWI698520 B TW I698520B TW 103144476 A TW103144476 A TW 103144476A TW 103144476 A TW103144476 A TW 103144476A TW I698520 B TWI698520 B TW I698520B
Authority
TW
Taiwan
Prior art keywords
oil
cell composition
sec
cells
microbial
Prior art date
Application number
TW103144476A
Other languages
English (en)
Chinese (zh)
Other versions
TW201529832A (zh
Inventor
馬克 巴克
金吉爾 夏恩客
老奇爾特Ｌ 馬修斯
納斯潤 塔巴業尼杰
尼爾Ｆ 萊尼格
Original Assignee
荷蘭商Ｄｓｍ智慧財產有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 荷蘭商Ｄｓｍ智慧財產有限公司 filed Critical 荷蘭商Ｄｓｍ智慧財產有限公司
Publication of TW201529832A publication Critical patent/TW201529832A/zh
Application granted granted Critical
Publication of TWI698520B publication Critical patent/TWI698520B/zh

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS OR COOKING OILS
    • A23D9/00—Other edible oils or fats, e.g. shortenings or cooking oils
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C11B1/02—Pretreatment
    • C11B1/025—Pretreatment by enzymes or microorganisms, living or dead
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
    • C07C51/42—Separation; Purification; Stabilisation; Use of additives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
    • C07C51/42—Separation; Purification; Stabilisation; Use of additives
    • C07C51/48—Separation; Purification; Stabilisation; Use of additives by liquid-liquid treatment
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
    • C07C51/42—Separation; Purification; Stabilisation; Use of additives
    • C07C51/487—Separation; Purification; Stabilisation; Use of additives by treatment giving rise to chemical modification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C57/00—Unsaturated compounds having carboxyl groups bound to acyclic carbon atoms
    • C07C57/02—Unsaturated compounds having carboxyl groups bound to acyclic carbon atoms with only carbon-to-carbon double bonds as unsaturation
    • C07C57/03—Monocarboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00—Refining fats or fatty oils
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00—Refining fats or fatty oils
    • C11B3/02—Refining fats or fatty oils by chemical reaction
    • C11B3/06—Refining fats or fatty oils by chemical reaction with bases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C12N1/066—Lysis of microorganisms by physical methods
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fats And Perfumes (AREA)
  • Enzymes And Modification Thereof (AREA)
TW103144476A 2013-12-20 2014-12-19 用於從微生物細胞獲得微生物油之方法（三） TWI698520B (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361919000P 2013-12-20 2013-12-20
US61/919,000 2013-12-20

Publications (2)

Publication Number Publication Date
TW201529832A TW201529832A (zh) 2015-08-01
TWI698520B true TWI698520B (zh) 2020-07-11

Family

ID=53403891

Family Applications (1)

Application Number Title Priority Date Filing Date
TW103144476A TWI698520B (zh) 2013-12-20 2014-12-19 用於從微生物細胞獲得微生物油之方法（三）

Country Status (15)

Country Link
US (1) US10364207B2 (cg-RX-API-DMAC7.html)
EP (1) EP3082794B1 (cg-RX-API-DMAC7.html)
JP (3) JP2017504318A (cg-RX-API-DMAC7.html)
KR (1) KR102435269B1 (cg-RX-API-DMAC7.html)
CN (1) CN106061475B (cg-RX-API-DMAC7.html)
AR (2) AR098891A1 (cg-RX-API-DMAC7.html)
AU (1) AU2014369045B2 (cg-RX-API-DMAC7.html)
BR (1) BR112016014516B1 (cg-RX-API-DMAC7.html)
CA (1) CA2934506C (cg-RX-API-DMAC7.html)
IL (1) IL246342B (cg-RX-API-DMAC7.html)
MX (1) MX2016008228A (cg-RX-API-DMAC7.html)
NZ (1) NZ721413A (cg-RX-API-DMAC7.html)
SG (1) SG11201605052XA (cg-RX-API-DMAC7.html)
TW (1) TWI698520B (cg-RX-API-DMAC7.html)
WO (1) WO2015095693A2 (cg-RX-API-DMAC7.html)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20080007279A (ko) 2000-01-19 2008-01-17 마텍 바이오싸이언스스 코포레이션 무용매 추출 방법
EP3617318A1 (en) 2010-06-01 2020-03-04 DSM IP Assets B.V. Extraction of lipid from cells and products therefrom
AR098890A1 (es) 2013-12-20 2016-06-22 Dsm Ip Assets Bv Proceso para obtener aceite microbiano a partir de células microbianas
AU2014369042B2 (en) 2013-12-20 2020-04-30 Dsm Ip Assets B.V. Processes for obtaining microbial oil from microbial cells
KR102435269B1 (ko) 2013-12-20 2022-08-22 디에스엠 아이피 어셋츠 비.브이. 미생물 세포로부터의 미생물 오일의 수득 방법
CA2934509C (en) 2013-12-20 2022-05-03 Neil Francis Leininger Processes for obtaining microbial oil from microbial cells
US11124736B2 (en) 2013-12-20 2021-09-21 Dsm Ip Assets B.V. Processes for obtaining microbial oil from microbial cells
WO2018011286A1 (en) * 2016-07-13 2018-01-18 Evonik Degussa Gmbh Method of separating lipids from a lysed lipids containing biomass
KR102405390B1 (ko) 2016-07-13 2022-06-03 에보닉 오퍼레이션스 게엠베하 용해된 지질을 함유하는 바이오매스로부터 지질을 분리하는 방법
CA3030467C (en) * 2016-07-13 2023-07-11 Evonik Degussa Gmbh Method for isolating lipids from lipid-containing cells
WO2018013670A1 (en) * 2016-07-13 2018-01-18 Dsm Ip Assets B.V. Method for extracting a microbial oil comprising polyunsaturated fatty acids from a fermentation broth containing oleaginous microorganisms
RU2744913C2 (ru) 2016-12-27 2021-03-17 Эвоник Оперейшенс ГмбХ Способ выделения липидов из липидсодержащей биомассы
EP3470502A1 (en) 2017-10-13 2019-04-17 Evonik Degussa GmbH Method of separating lipids from a lysed lipids containing biomass
EP3527664A1 (en) 2018-02-15 2019-08-21 Evonik Degussa GmbH Method of isolating lipids from a lipids containing biomass
CA3094477A1 (en) * 2018-03-30 2019-10-03 Dsm Ip Assets B.V. Method of obtaining a microbial oil and a method of reducing emulsion by maintaining a low concentration of carbohydrate
BR112020023222A2 (pt) 2018-05-15 2021-03-23 Evonik Operations Gmbh método de isolamento de lipídios a partir de uma biomassa contendo lipídios lisados por inversão por emulsão
DK3794097T3 (da) 2018-05-15 2024-12-16 Evonik Operations Gmbh Fremgangsmåde til isolering af lipider fra en lipid-indeholdende biomasse ved hjælp af hydrofob silica
US11312976B2 (en) * 2018-06-29 2022-04-26 Amyris, Inc. Methods for recovering water-immiscible isoprenoid compounds from microbial biomass
EP3696273A1 (en) * 2019-02-18 2020-08-19 Technische Universität München A method for producing microbial lipids
KR20210132716A (ko) * 2019-03-14 2021-11-04 디에스엠 아이피 어셋츠 비.브이. 효소 및 pH 충격에 의한 미생물 세포 조성물로부터의 지질 수득 방법
WO2020186128A1 (en) * 2019-03-14 2020-09-17 Dsm Ip Assets B.V. Methods of obtaining lipids from a microbial cell composition
CN113684088B (zh) * 2020-05-18 2024-06-11 嘉必优生物技术（武汉）股份有限公司 一种微生物油脂提取方法及其所得微生物油脂
EP3933016A1 (en) * 2020-06-30 2022-01-05 Evonik Operations GmbH Method of isolating lipids from a lipids containing biomass

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101610824A (zh) * 2006-12-22 2009-12-23 丹尼斯科美国公司 酶辅助的水性脂类提取物的脱乳化作用
CN103124791A (zh) * 2010-06-01 2013-05-29 帝斯曼知识产权资产管理有限公司 从细胞中提取脂质以及由此获得的产品

Family Cites Families (153)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2753362A (en) 1951-05-18 1956-07-03 Standard Brands Inc Process of extracting lipids from plant and animal tissue
GB808128A (en) 1955-12-01 1959-01-28 Nat Res Dev A method of increasing the fatty contents of such micro-organisms as yeasts, bacteria and moulds
US3089821A (en) 1959-10-28 1963-05-14 Merck & Co Inc Process for the preparation of lipids
DE2056896B2 (de) 1970-02-18 1979-12-06 Veb Schwermaschinenbau Kombinat Ernst Thaelmann Magdeburg, Ddr 3011 Magdeburg Verfahren zur gleichzeitigen Gewinnung von Fett und Protein aus pflanzlichen, ölhaltigen Rohstoffen und Zwischenprodukten
US3878232A (en) 1970-09-28 1975-04-15 Staley Mfg Co A E Extraction process to improve the quality and yield of crude vegetable oils
GB1466853A (en) 1973-05-22 1977-03-09 Simon Rosedowns Ltd Extraction
US4504473A (en) 1982-06-30 1985-03-12 Ribi Immunochem Research, Inc. Pyridine soluble extract of a microorganism
DE3248167A1 (de) 1982-12-27 1984-06-28 Wintershall Ag, 3100 Celle Trehaloselipidtetraester
DE3587044T2 (de) 1985-01-22 1993-06-03 Japan As Represented By Direct Verfahren zur herstellung von lipiden aus fungusmassen.
JPS61170397A (ja) 1985-01-22 1986-08-01 Agency Of Ind Science & Technol モルテイエレラ属糸状菌体の多段抽出処理方法
US4792418A (en) 1985-08-14 1988-12-20 Century Laboratories, Inc. Method of extraction and purification of polyunsaturated fatty acids from natural sources
JPS6244170A (ja) 1985-08-19 1987-02-26 Agency Of Ind Science & Technol モルテイエレラ属糸状菌体の超臨界流体による抽出方法
US4680314A (en) 1985-08-30 1987-07-14 Microbio Resources, Inc. Process for producing a naturally-derived carotene/oil composition by direct extraction from algae
JPS62278987A (ja) 1986-05-26 1987-12-03 Kanegafuchi Chem Ind Co Ltd 酵素反応生成物の回収方法
DK199887D0 (da) 1987-04-15 1987-04-15 Danisco Bioteknologi As Gaerstamme
JPS63304990A (ja) 1987-06-04 1988-12-13 Meiji Seika Kaisha Ltd 菌体内有効成分の抽出方法
FR2621327B1 (fr) 1987-10-06 1990-01-05 Commissariat Energie Atomique Procede de production et d'extraction de polysaccharides a partir d'une culture de porphyridium cruentum et dispositif pour la mise en oeuvre du procede
JPH0198494A (ja) 1987-10-09 1989-04-17 Agency Of Ind Science & Technol バイオリアクター
US5340742A (en) 1988-09-07 1994-08-23 Omegatech Inc. Process for growing thraustochytrium and schizochytrium using non-chloride salts to produce a microfloral biomass having omega-3-highly unsaturated fatty acids
US5130242A (en) 1988-09-07 1992-07-14 Phycotech, Inc. Process for the heterotrophic production of microbial products with high concentrations of omega-3 highly unsaturated fatty acids
US5340594A (en) 1988-09-07 1994-08-23 Omegatech Inc. Food product having high concentrations of omega-3 highly unsaturated fatty acids
US6451567B1 (en) 1988-09-07 2002-09-17 Omegatech, Inc. Fermentation process for producing long chain omega-3 fatty acids with euryhaline microorganisms
US5173409A (en) 1989-12-08 1992-12-22 Ecogen Inc. Recovery of bt endotoxin protein from lysed cell mixtures
US5288619A (en) 1989-12-18 1994-02-22 Kraft General Foods, Inc. Enzymatic method for preparing transesterified oils
FR2656874B1 (fr) 1990-01-11 1992-04-03 Commissariat Energie Atomique Procede de production et d'extraction d'anti-oxydants a partir d'une culture de micro-organismes et photobioreacteur pour la mise en óoeuvre de ce procede.
US5407957A (en) 1990-02-13 1995-04-18 Martek Corporation Production of docosahexaenoic acid by dinoflagellates
US5133963A (en) 1990-12-21 1992-07-28 Shuntaro Ise Method of producing commercially useful poultry products with increased concentrations of Omega-3 polyunsaturated fatty acids
US5658767A (en) 1991-01-24 1997-08-19 Martek Corporation Arachidonic acid and methods for the production and use thereof
US6270828B1 (en) 1993-11-12 2001-08-07 Cargrill Incorporated Canola variety producing a seed with reduced glucosinolates and linolenic acid yielding an oil with low sulfur, improved sensory characteristics and increased oxidative stability
FR2686619B1 (fr) 1992-01-28 1995-07-13 Commissariat Energie Atomique Procede de production selective de lipides poly-insatures a partir d'une culture de micro-algues du type porphyridium et cuve utilisee dans ce procede.
US5476787A (en) 1992-04-24 1995-12-19 Director-General Of Agency Of Industrial Science And Technology Method of removing nitrogen impurities from water using hydrocarbon-producing microalga
DE4219360C2 (de) 1992-06-12 1994-07-28 Milupa Ag Verfahren zur Gewinnung von Lipiden mit einem hohen Anteil von langkettig-hochungesättigten Fettsäuren
WO1994024849A1 (en) 1993-04-27 1994-11-10 Cargill, Incorporated Non-hydrogenated canola oil for food applications
FR2719222B1 (fr) 1994-05-02 1996-06-21 Rocher Yves Biolog Vegetale Vésicules lipidiques, leur procédé de fabrication et leurs applications.
EP0776356B1 (de) 1994-08-16 1999-06-30 Dr. Frische GmbH Verfahren zur gewinnung von nicht wasserlöslichen, nativen produkten aus nativen stoffgemengen mit hilfe der zentrifugalkraft
US5583019A (en) 1995-01-24 1996-12-10 Omegatech Inc. Method for production of arachidonic acid
US6582941B1 (en) 1995-04-17 2003-06-24 Japan As Represented By Director-General Of Agency Of Industrial Science And Technology Microorganisms capable of producing highly unsaturated fatty acids and process for producing highly unsaturated fatty acids by using the microorganisms
ES2130515T3 (es) 1995-05-04 1999-07-01 Nestle Sa Procedimiento para fraccionar acidos grasos.
JPH099981A (ja) 1995-06-28 1997-01-14 Sekiyu Sangyo Kasseika Center 油水二相系微生物反応における油水分離方法
GB9514649D0 (en) 1995-07-18 1995-09-13 Zeneca Ltd Extraction of triglycerides from microorganisms
JP3985035B2 (ja) 1995-09-14 2007-10-03 独立行政法人産業技術総合研究所 （ｎ−６）系ドコサペンタエン酸含有油脂ならびに該油脂の製造方法および用途
CN104017734A (zh) 1996-03-28 2014-09-03 Dsmip资产有限公司 粒状微生物生物质的制备及其有价值的化合物的分离方法
EP2280062A3 (en) 1996-03-28 2011-09-28 DSM IP Assets B.V. Preparation of microbial polyunsaturated fatty acid containing oil from pasteurised biomass
US6255505B1 (en) 1996-03-28 2001-07-03 Gist-Brocades, B.V. Microbial polyunsaturated fatty acid containing oil from pasteurised biomass
JP2000510513A (ja) 1996-05-15 2000-08-15 ヒスト―ブロカデス・ベスローテン・フェンノートシャップ 低ステロール且つ高トリグリセライドの微生物性油を得るための極性溶媒によるステロール抽出
WO1998001536A1 (en) 1996-07-03 1998-01-15 Sagami Chemical Research Center Microorganisms producing docosahexaenoic acid and process for the production of docosahexaenoic acid
JP3343358B2 (ja) 1996-07-23 2002-11-11 ナガセケムテックス株式会社 ドコサヘキサエン酸およびドコサペンタエン酸の製造方法
US5951875A (en) 1996-12-20 1999-09-14 Eastman Chemical Company Adsorptive bubble separation methods and systems for dewatering suspensions of microalgae and extracting components therefrom
GB9701705D0 (en) 1997-01-28 1997-03-19 Norsk Hydro As Purifying polyunsatured fatty acid glycerides
EP0972844B1 (en) 1997-03-04 2008-09-17 Suntory Limited Process for preparing highly unsaturated fatty acid and lipid containing highly unsaturated fatty acid
AU7530598A (en) 1997-05-02 1998-11-27 Dsm N.V. Isolation of carotenoid crystals from microbial biomass
US7585645B2 (en) 1997-05-27 2009-09-08 Sembiosys Genetics Inc. Thioredoxin and thioredoxin reductase containing oil body based products
EP1905309A1 (en) 1997-05-27 2008-04-02 SemBioSys Genetics Inc. Uses of oil bodies
US6566583B1 (en) 1997-06-04 2003-05-20 Daniel Facciotti Schizochytrium PKS genes
JP3836231B2 (ja) 1997-10-17 2006-10-25 日本化学飼料株式会社 ホタテガイ中腸腺から得られる高度不飽和脂肪酸含有油及びその製造方法
EP0987926B1 (en) 1997-11-12 2004-04-28 Mitsubishi Denki Kabushiki Kaisha An electroluminescent light
EP1042451A4 (en) 1997-12-23 2001-07-04 Dcv Inc LINOLEAT ISOMERASE
US6127185A (en) 1998-05-05 2000-10-03 University Of Tennessee Research Corporation Instrument and method for measurement of stability of oils
JP2000041684A (ja) 1998-07-29 2000-02-15 Daicel Chem Ind Ltd 新規なｄ−アミノアシラーゼおよびその製造方法、並びに該ｄ−アミノアシラーゼを利用したｄ−アミノ酸の製造方法
JP2000135096A (ja) 1998-08-28 2000-05-16 Tadayuki Imanaka 界面活性剤、その生産方法およびその利用方法
FR2782921B1 (fr) 1998-09-09 2002-09-20 Dior Christian Parfums Extrait lipidique de l'algue skeletonema, procede de preparation et utilisation dans des domaines cosmetique et pharmaceutique, notamment dermatologique
US6166231A (en) 1998-12-15 2000-12-26 Martek Biosciences Corporation Two phase extraction of oil from biomass
CA2260397A1 (en) 1999-01-29 2000-07-29 Atlantis Marine Inc. Method of converting rendered triglyceride oil from marine sources into bland, stable food oil
JP2000245492A (ja) 1999-03-02 2000-09-12 Kyowa Hakko Kogyo Co Ltd 微生物抽出脂質
KR19990046733A (ko) 1999-04-20 1999-07-05 류성구 미생물슈도모나스에의한도코사핵사노인산(ｄｈａ)의제조방법
US6344349B1 (en) 1999-12-06 2002-02-05 Decant Technologies Llc Process and system for electrical extraction of intracellular matter from biological matter
KR20080007279A (ko) 2000-01-19 2008-01-17 마텍 바이오싸이언스스 코포레이션 무용매 추출 방법
KR20070073986A (ko) 2000-01-28 2007-07-10 마텍 바이오싸이언스스 코포레이션 발효기 내에서 진핵 미생물의 고밀도 배양에 의한고도불포화 지방산을 함유하는 지질의 증진된 생산 방법
MXPA02010005A (es) 2000-04-12 2004-08-19 Westfalia Separator Ag Metodos para el fraccionamiento de materias primas nativas que contienen aceite y lipidos polares.
DE10018213A1 (de) 2000-04-12 2001-10-25 Westfalia Separator Ind Gmbh Verfahren zur Fraktionierung von öl-und lecithinhaltigen nativen Rohstoffen
EP1178103A1 (en) 2000-08-02 2002-02-06 Dsm N.V. Purifying crude pufa oils
EP1178118A1 (en) * 2000-08-02 2002-02-06 Dsm N.V. Isolation of microbial oils
US20060060520A1 (en) 2001-06-25 2006-03-23 Bomberger David C Systems and methods using a solvent for the removal of lipids from fluids
US20030060509A1 (en) 2001-08-24 2003-03-27 Elswyk Mary Van Products containing highly unsaturated fatty acids for use by women and their children during stages of preconception, pregnancy and lactation/post-partum
CN1267174C (zh) 2001-12-12 2006-08-02 马泰克生物科学博尔德公司 从含油种子和微生物来源提取和冬化脂类的方法
CN1177024C (zh) 2002-03-27 2004-11-24 西南农业大学 水剂法生产食用菜籽蛋白和油脂的方法
EP2921155B1 (en) 2002-05-03 2020-01-08 DSM IP Assets B.V. Methods for producing high-quality lipids by enzymatic liberation from biomass
WO2004001021A1 (en) 2002-06-19 2003-12-31 Dsm Ip Assets B.V. Pasteurisation process for microbial cells and microbial oil
CN103834699A (zh) 2003-10-02 2014-06-04 Dsmip资产公司 使用改进量的氯和钾在微藻类中产生高水平的dha
DK1706500T3 (da) 2003-12-30 2010-12-13 Dsm Ip Assets Bv Afluftningsfremgangsmåde
US7038559B2 (en) 2004-02-23 2006-05-02 Ruby Richard C Vertically separated acoustic filters and resonators
KR101194236B1 (ko) 2004-03-01 2012-10-29 산토리 홀딩스 가부시키가이샤 장쇄 고도 불포화 지방산을 구성요소로서 포함하는 인지질 및 그 이용
CA2559496A1 (en) 2004-04-27 2005-11-17 Baxter International Inc. Stirred-tank reactor system
EP1597976B1 (en) 2004-05-21 2013-01-30 Nestec S.A. Use of polyol esters of fatty acids in aerated frozen confection with improved nutritional attributes
WO2006046943A2 (en) 2004-10-22 2006-05-04 Martek Biosciences Corporation Methods for producing lipids by liberation from biomass
DE102004062141A1 (de) 2004-12-23 2006-07-06 Nutrinova Nutrition Specialties & Food Ingredients Gmbh Verfahren zur Herstellung eines Rohöls aus Gemischen von Mikroorganismen und Pflanzen, das so hergestellte Öl sowie die spezifischen Verwendungen des so hergestellten und gegebenenfalls zusätzlich raffinierten Öls
DE102005003624A1 (de) 2005-01-26 2006-07-27 Nutrinova Nutrition Specialties & Food Ingredients Gmbh Herstellung und Anwendung eines antioxidativ wirksamen Extraktes aus Crypthecodinium sp.
NZ563125A (en) 2005-06-03 2009-11-27 Mcfarlane Marketing Aust Pty L Lipid extract of mussels and method for preparation thereof
EP2447356B1 (en) 2005-06-07 2016-04-20 DSM Nutritional Products AG Eukaryotic microorganisms for producing lipids and antioxidants
EP1903883A4 (en) 2005-07-01 2010-06-23 Martek Biosciences Corp OIL PRODUCT CONTAINING MULTIPLE UNSATURATED FATTY ACIDS AND ITS USES AND MANUFACTURING
US7527734B1 (en) 2005-11-15 2009-05-05 Shepherd Samuel L Rapid non-equilibrium decompression of microorganism-containing waste streams
US20090221705A1 (en) 2006-02-07 2009-09-03 Pal Rongved Omega 3
JP5658876B2 (ja) 2006-07-05 2015-01-28 フォトンズ コーポレーション リミテッド 大規模従属栄養培養領域にて産生される超高純度ｅｐａと極性脂質
EP1887011A1 (en) 2006-08-09 2008-02-13 Thermphos Trading GmbH Alpha amino acid phosphonic acid compounds, method of preparation and use thereof
EP2074214A2 (en) 2006-09-28 2009-07-01 Microbia, Inc. Production of sterols in oleaginous yeast and fungi
KR100810314B1 (ko) 2006-10-11 2008-03-04 삼성전자주식회사 휴대 단말기의 키 입력 장치
CN101224022B (zh) 2007-01-15 2012-10-31 天津科技大学 水酶法同时制备芝麻油和蛋白质的工艺方法
WO2008151373A1 (en) 2007-06-14 2008-12-18 Fuji Fuels Pty Ltd Algae growth for biofuels
EP2732709A1 (en) 2007-08-29 2014-05-21 Aker BioMarine AS A new method for making krill meal
US20100226977A1 (en) 2007-08-29 2010-09-09 Aker Biomarine Asa Low viscosity phospholipid compositions
KR101215416B1 (ko) 2008-03-26 2012-12-26 비와이디 컴퍼니 리미티드 리튬 배터리용 캐쏘드 물질
ITMI20081203A1 (it) 2008-06-30 2010-01-01 Eni Spa Procedimento per l'estrazione di acidi grassi da biomassa algale
EP2145942A1 (de) 2008-07-15 2010-01-20 Lonza Ltd. Verfahren zur Isolierung von Ölen aus Zellen und Biomasse
WO2010017243A1 (en) 2008-08-04 2010-02-11 Kai Bioenergy Continuous cultivation, harvesting, and oil extraction of photosynthetic cultures
US8815570B2 (en) 2008-10-02 2014-08-26 Feyecon B.V. Microalgae extract containing omega 3-polyunsaturated fatty acids and method for extracting oil from micro-organisms
US9896642B2 (en) 2008-10-14 2018-02-20 Corbion Biotech, Inc. Methods of microbial oil extraction and separation
CN104770424A (zh) 2008-10-14 2015-07-15 索拉兹米公司 微藻生物质的食品组合物
CN101455240B (zh) 2008-12-29 2011-04-06 东北农业大学 水酶法提取南瓜籽油的方法
SE534278C2 (sv) 2009-02-17 2011-06-28 Alfa Laval Corp Ab Ett kontinuerligt förfarande för isolering av oljor från alger eller mikroorganismer
MX2011009105A (es) 2009-03-10 2011-09-28 Srs Energy Fraccionamiento de biomasa de algas.
US9296985B2 (en) 2009-03-10 2016-03-29 Valicor, Inc. Algae biomass fractionation
US8207363B2 (en) 2009-03-19 2012-06-26 Martek Biosciences Corporation Thraustochytrids, fatty acid compositions, and methods of making and uses thereof
ES2757878T3 (es) 2009-03-19 2020-04-30 Dsm Ip Assets Bv Composiciones de ácidos grasos de traustoquítridos y métodos de fabricación y usos de las mismas
JP2012115145A (ja) * 2009-03-30 2012-06-21 Kaneka Corp ポリヒドロキシアルカノエートの回収方法
US8476060B2 (en) 2009-04-13 2013-07-02 Board Of Regents, The University Of Texas System Process for separating lipids from a biomass
SG10201401472YA (en) 2009-04-14 2014-08-28 Solazyme Inc Methods Of Microbial Oil Extraction And Separation
CN101531690B (zh) 2009-04-28 2011-09-14 浙江神茗山茶业科技有限公司 一种用水作溶剂直接从茶叶籽仁中提取茶皂素和茶叶籽油的工艺
MX2011012543A (es) 2009-05-26 2012-04-02 Solazyme Inc Fraccionamiento de la biomasa microbiana que contiene aceite.
KR101659765B1 (ko) 2009-09-28 2016-09-27 삼성전자주식회사 다중 모드 휴대용 단말기에서 전력 소모를 줄이기 위한 장치 및 방법
WO2011059745A1 (en) 2009-10-28 2011-05-19 The Arizona Board Of Regents For And On Behalf Of Arizona State University Bacterium for production of fatty acids
JP5920890B2 (ja) 2010-01-19 2016-05-18 ディーエスエム アイピー アセッツ ビー．ブイ． エイコサペンタエン酸生成微生物、脂肪酸組成物、ならびにそれらを作る方法およびそれらの使用
BR112012026242A2 (pt) 2010-04-14 2019-09-24 Solazyme Inc método para produção de óleo e óleo isolado de levedura oleaginosa
WO2011130576A1 (en) 2010-04-14 2011-10-20 Solazyme, Inc. Oleaginous yeast food compositions
CA2795460A1 (en) 2010-04-22 2011-10-27 E. I. Du Pont De Nemours And Company Method for obtaining polyunsaturated fatty acid-containing compositions from microbial biomass
SG185578A1 (en) 2010-06-14 2013-01-30 Io Mega Holding Corp Methods for the production of algae derived oils
BR112013002003A2 (pt) 2010-07-26 2019-09-24 Sapphire Energy Inc processo para recuperação de compostos oleaginosos de biomassa
US9028696B2 (en) 2010-07-26 2015-05-12 Sapphire Energy, Inc. Process for the recovery of oleaginous compounds from biomass
US8192627B2 (en) 2010-08-06 2012-06-05 Icm, Inc. Bio-oil recovery methods
US20120040428A1 (en) 2010-08-13 2012-02-16 Paul Reep Procedure for extracting of lipids from algae without cell sacrifice
US20120129244A1 (en) 2010-10-17 2012-05-24 Michael Phillip Green Systems, methods and apparatuses for dewatering, flocculating and harvesting algae cells
MX2012012250A (es) 2010-10-18 2013-03-05 Originoil Inc Sistemas, aparatos, y metodos para extraer lipidos no polares de una lechada acuosa de algas y lipidos producidos de la misma.
CN101985637B (zh) 2010-11-02 2014-05-07 嘉必优生物工程（武汉）有限公司 一种微生物油脂的提取方法
WO2012061647A2 (en) 2010-11-03 2012-05-10 Solazyme, Inc. Microbial oils with lowered pour points, dielectric fluids produced therefrom, and related methods
DK2450425T3 (da) 2010-11-08 2014-07-07 Neste Oil Oyj Fremgangsmåde til lipidekstraktion fra biomasse
WO2012109642A1 (en) 2011-02-12 2012-08-16 Phycal, Inc. Aqueous extraction methods for high lipid microorganisms
CN103649313B (zh) 2011-03-07 2017-10-24 Dsm营养产品股份公司 工程化破囊壶菌属微生物
US20120238732A1 (en) 2011-03-15 2012-09-20 Iowa State University Research Foundation, Inc. Oil extraction from microalgae
FR2975705B1 (fr) 2011-05-27 2014-12-26 Roquette Freres Procede d'extraction du squalene a partir de microalgues
CN102433215A (zh) 2011-09-22 2012-05-02 厦门汇盛生物有限公司 一种从真菌或藻类中物理破壁提取油脂的方法
JP2013116101A (ja) * 2011-10-31 2013-06-13 Kohjin Life Sciences Co Ltd 食物繊維含量の高い酵母細胞壁画分
CN102388988B (zh) 2011-11-08 2013-01-23 中国农业科学院油料作物研究所 一种微生物油的分提方法
WO2013075116A2 (en) 2011-11-17 2013-05-23 Heliae Development, Llc Omega 7 rich compositions and methods of isolating omega 7 fatty acids
EP2838630A2 (fr) 2012-04-16 2015-02-25 Roquette Frères Procédé de raffinage du squalène produit par microalgues
US8980590B2 (en) 2012-06-29 2015-03-17 BP Biofuels UK Limited Process for separation of renewable materials from microorganisms
AU2014369042B2 (en) 2013-12-20 2020-04-30 Dsm Ip Assets B.V. Processes for obtaining microbial oil from microbial cells
US11124736B2 (en) 2013-12-20 2021-09-21 Dsm Ip Assets B.V. Processes for obtaining microbial oil from microbial cells
CA2934509C (en) 2013-12-20 2022-05-03 Neil Francis Leininger Processes for obtaining microbial oil from microbial cells
PT3626806T (pt) 2013-12-20 2024-07-31 Mara Renewables Corp Métodos para recuperar óleo de microrganismos
AR098890A1 (es) 2013-12-20 2016-06-22 Dsm Ip Assets Bv Proceso para obtener aceite microbiano a partir de células microbianas
SG11201605061TA (en) 2013-12-20 2016-08-30 Dsm Ip Assets Bv Process for extracting lipids for use in production of biofuels
KR102435268B1 (ko) 2013-12-20 2022-08-22 디에스엠 뉴트리셔널 프라덕츠 아게 미생물로부터 오일을 회수하는 방법
KR102435269B1 (ko) 2013-12-20 2022-08-22 디에스엠 아이피 어셋츠 비.브이. 미생물 세포로부터의 미생물 오일의 수득 방법
CA3216668A1 (en) 2014-07-07 2016-01-14 Nuseed Global Innovation Ltd Processes for producing industrial products from plant lipids

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101610824A (zh) * 2006-12-22 2009-12-23 丹尼斯科美国公司 酶辅助的水性脂类提取物的脱乳化作用
CN103124791A (zh) * 2010-06-01 2013-05-29 帝斯曼知识产权资产管理有限公司 从细胞中提取脂质以及由此获得的产品

Also Published As

Publication number Publication date
CN106061475A (zh) 2016-10-26
US10364207B2 (en) 2019-07-30
JP2022101667A (ja) 2022-07-06
CN106061475B (zh) 2022-03-25
AR098893A1 (es) 2016-06-22
TW201529832A (zh) 2015-08-01
EP3082794A4 (en) 2017-07-26
BR112016014516A2 (cg-RX-API-DMAC7.html) 2017-08-08
JP7381649B2 (ja) 2023-11-15
CA2934506C (en) 2022-05-03
WO2015095693A3 (en) 2015-10-29
WO2015095693A2 (en) 2015-06-25
JP2017504318A (ja) 2017-02-09
US20160318840A1 (en) 2016-11-03
AR098891A1 (es) 2016-06-22
IL246342A0 (en) 2016-08-31
AU2014369045B2 (en) 2020-02-27
JP2020031652A (ja) 2020-03-05
MX2016008228A (es) 2016-11-28
AU2014369045A1 (en) 2016-07-07
IL246342B (en) 2021-10-31
NZ721413A (en) 2022-05-27
KR20160100382A (ko) 2016-08-23
SG11201605052XA (en) 2016-07-28
CA2934506A1 (en) 2015-06-25
EP3082794A2 (en) 2016-10-26
KR102435269B1 (ko) 2022-08-22
EP3082794B1 (en) 2024-05-08
BR112016014516B1 (pt) 2022-02-22

Similar Documents

Publication Publication Date Title
JP7381649B2 (ja) 微生物細胞から微生物油を入手するための方法
JP7487962B2 (ja) 微生物細胞から微生物油を入手するための方法
US12104139B2 (en) Processes for obtaining microbial oil from microbial cells
TWI708836B (zh) 用於從微生物細胞獲得微生物油之方法（二）
TWI646188B (zh) 用於從微生物細胞獲得微生物油之方法（四）
HK1230088A1 (en) Processes for obtaining microbial oil from microbial cells
HK1230088B (en) Processes for obtaining microbial oil from microbial cells