SI20693B - Humana hibridna gostiteljska celica za ekspresijosesalskih genov - Google Patents
Humana hibridna gostiteljska celica za ekspresijosesalskih genov Download PDFInfo
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- SI20693B SI20693B SI9920096A SI9920096A SI20693B SI 20693 B SI20693 B SI 20693B SI 9920096 A SI9920096 A SI 9920096A SI 9920096 A SI9920096 A SI 9920096A SI 20693 B SI20693 B SI 20693B
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- 230000014509 gene expression Effects 0.000 title claims abstract 4
- 210000004027 cell Anatomy 0.000 claims abstract 30
- 108090000623 proteins and genes Proteins 0.000 claims abstract 15
- 108010064593 Intercellular Adhesion Molecule-1 Proteins 0.000 claims abstract 2
- 102000004169 proteins and genes Human genes 0.000 claims 14
- 102100026735 Coagulation factor VIII Human genes 0.000 claims 2
- 101000911390 Homo sapiens Coagulation factor VIII Proteins 0.000 claims 2
- 230000007910 cell fusion Effects 0.000 claims 2
- 210000004978 chinese hamster ovary cell Anatomy 0.000 claims 2
- 238000000034 method Methods 0.000 claims 2
- 108090000144 Human Proteins Proteins 0.000 claims 1
- 102000003839 Human Proteins Human genes 0.000 claims 1
- 102000015271 Intercellular Adhesion Molecule-1 Human genes 0.000 claims 1
- 230000013595 glycosylation Effects 0.000 claims 1
- 238000006206 glycosylation reaction Methods 0.000 claims 1
- 210000005260 human cell Anatomy 0.000 claims 1
- 238000012216 screening Methods 0.000 claims 1
- 230000003248 secreting effect Effects 0.000 claims 1
- 230000004927 fusion Effects 0.000 abstract 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 abstract 2
- 210000003292 kidney cell Anatomy 0.000 abstract 2
- 208000011691 Burkitt lymphomas Diseases 0.000 abstract 1
- 108060003951 Immunoglobulin Proteins 0.000 abstract 1
- 102000004877 Insulin Human genes 0.000 abstract 1
- 108090001061 Insulin Proteins 0.000 abstract 1
- 102100037877 Intercellular adhesion molecule 1 Human genes 0.000 abstract 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract 1
- 210000003719 b-lymphocyte Anatomy 0.000 abstract 1
- 210000004754 hybrid cell Anatomy 0.000 abstract 1
- 102000018358 immunoglobulin Human genes 0.000 abstract 1
- 229940125396 insulin Drugs 0.000 abstract 1
- 238000003259 recombinant expression Methods 0.000 abstract 1
- 238000004114 suspension culture Methods 0.000 abstract 1
- 238000001890 transfection Methods 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/02—Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
- C12N5/12—Fused cells, e.g. hybridomas
- C12N5/16—Animal cells
- C12N5/163—Animal cells one of the fusion partners being a B or a T lymphocyte
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/02—Cells for production
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
Humane/humane hibridne celice so bile narejene s fuzijo humanih embrionalnih ledvičnih celic (293S) in modificiranih celic Burkittovega limfoma (2B8). Fuzijske celice so uporabne kot gostiteljske celice za rekombinantno ekspresijo sesalskih genov. Prednosti uporabe teh hibridnih klonov humanih ledvičnih in B-celic, imenovanih HKB, za ekspresijo sesalskih genov vključujejo: (i) celice so negativne za imunoglobulinsko ekspresijo, (ii) celice zlahka rastejo v plazemskem mediju brez proteina (z dodatkom rekombinantnega inzulina ali brez njega) kot suspenzijskih kulturah v stresalni steklenici ali v fermentorju, (iii) celice so zelo občutljive za transfekcijo DNA in (iv) celice izločajo visoke nivoje heterolognih rekombinantnih proteinov, kot npr. rekombinantna monoklonska protitelesa, topne ICAM-1, rIL-4 in rFVIII.
Claims (16)
1 PATENTNI ZAHTEVKI 1. Celica (ATCC depozitna številka: CRL-12568), izvedena s fuzijo celice 293 s celico 2B8 (ATCC depozitna številka: CRL-12569).
2. Celica po zahtevku 1, označena s tem, da izraža heterologni protein.
3. Celica po zahtevku 2, označena s tem, daje heterologni protein izbran iz skupine, ki jo sestavljajo FVIII, BDD-FVIII, monoklonsko protitelo, anti-TNF protitelo, rIL4, tPA in EPO.
4. Celična linija HKB11 (ATCC depozitna številka: CRL-12568), ki je bila konstruirana, da izraža heterologni protein.
5. Celična linija po zahtevku 4, označena s tem, daje protein ICAM-1.
6. Celična linija po zahtevku 4, označena s tem, daje protein BDD-FVIII.
7. Celična linija po zahtevku 4, označena s tem, daje protein monoklonsko protitelo.
8. Celična linija po zahtevku 7, označena s tem, da je monoklonsko protitelo anti-TNF.
9. Celična linija po zahtevku 4, označena s tem, daje protein rIL4.
10. Celična linija po zahtevku 4, označena s tem, daje protein FVIII.
11. Celična linija po zahtevku 4, označena s tem, daje protein tPA.
12. Celična linija po zahtevku 4, označena s tem, daje protein EPO. 2
13. Celična linija po zahtevku 4, označena s tem, da je protein IL-4SA (T13D/R121E).
14. Celična linija po zahtevku 4, označena s tem, da je protein humani protein s humanim glikozilacijskim profilom.
15. Postopek za produkcijo hibridnih humanih celic, uporabnih za ekspresijo heterolognega proteina, označen s tem, da obsega stopnje: a) pridobivanje celic 293; b) pridobivanje celic 2B8 (ATCC depozitna številka: CTRL-12569); c) kontaktiranje celice iz stopnje a) s celicami iz stopnje b) v razmerah, ki dopuščajo, da pride do celične fuzije; d) presejanje celic, dobljenih v stopnji c), za celice, ki izločajo heterologni protein v višjih količinah kot ovarijske celice kitajskega hrčka (CHO-celice).
16. Postopek po zahtevku 15, označen s tem, da so celice humanega embrionalnega ledvičnega izvora celice 293S.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/209,920 US6136599A (en) | 1998-12-10 | 1998-12-10 | Human hybrid host cell for mammalian gene expression |
| PCT/US1999/029332 WO2000034462A1 (en) | 1998-12-10 | 1999-12-08 | Human hybrid host cell for mammalian gene expression |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| SI20693A SI20693A (sl) | 2002-04-30 |
| SI20693B true SI20693B (sl) | 2008-12-31 |
Family
ID=22780882
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SI9920096A SI20693B (sl) | 1998-12-10 | 1999-12-08 | Humana hibridna gostiteljska celica za ekspresijosesalskih genov |
Country Status (27)
| Country | Link |
|---|---|
| US (1) | US6136599A (sl) |
| EP (1) | EP1137768B1 (sl) |
| JP (3) | JP4332300B2 (sl) |
| KR (1) | KR100627753B1 (sl) |
| CN (1) | CN1230532C (sl) |
| AT (1) | ATE306547T1 (sl) |
| AU (1) | AU757188B2 (sl) |
| BG (1) | BG65263B1 (sl) |
| BR (1) | BRPI9916095B8 (sl) |
| CA (1) | CA2353698C (sl) |
| CU (2) | CU23382A3 (sl) |
| CZ (1) | CZ302677B6 (sl) |
| DE (1) | DE69927707T2 (sl) |
| DK (1) | DK1137768T3 (sl) |
| ES (1) | ES2249929T3 (sl) |
| HU (1) | HU229775B1 (sl) |
| ID (1) | ID30448A (sl) |
| IL (2) | IL143351A0 (sl) |
| NO (1) | NO330548B1 (sl) |
| PL (1) | PL205549B1 (sl) |
| RO (1) | RO121563B1 (sl) |
| RU (1) | RU2228355C2 (sl) |
| SI (1) | SI20693B (sl) |
| SK (1) | SK287888B6 (sl) |
| TR (1) | TR200101642T2 (sl) |
| UA (1) | UA77642C2 (sl) |
| WO (1) | WO2000034462A1 (sl) |
Families Citing this family (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6358703B1 (en) * | 1998-12-10 | 2002-03-19 | Bayer Corporation | Expression system for factor VIII |
| US6136599A (en) * | 1998-12-10 | 2000-10-24 | Bayer Corporation | Human hybrid host cell for mammalian gene expression |
| US6348192B1 (en) * | 1999-05-11 | 2002-02-19 | Bayer Corporation | Interleukin-2 mutein expressed from mammalian cells |
| US20040235715A1 (en) * | 1999-11-12 | 2004-11-25 | Bayer Corporation | Method of producing glycosylated bikunin |
| US6569657B1 (en) * | 2000-06-27 | 2003-05-27 | Millennium Pharmaceuticals, Inc. | 32140, a novel human aldehyde dehydrogenase and uses therefor |
| US7094587B2 (en) * | 2000-06-27 | 2006-08-22 | Millennium Pharmaceuticals, Inc. | 16002 Molecules and uses therefor |
| MY139948A (en) * | 2000-09-28 | 2009-11-30 | Bayer Corp | Enhanced transfection system |
| US7723102B2 (en) * | 2000-09-28 | 2010-05-25 | Bayer Corporation | Enhanced transfection system |
| US20060073141A1 (en) * | 2001-06-28 | 2006-04-06 | Domantis Limited | Compositions and methods for treating inflammatory disorders |
| KR100496861B1 (ko) | 2002-09-26 | 2005-06-22 | 삼성전자주식회사 | 하나의 핸들러에 2개 이상의 테스트 보드를 갖는 테스트장비 및 그 테스트 방법 |
| EP1578799B8 (en) | 2002-12-02 | 2011-03-23 | Amgen Fremont Inc. | Antibodies directed to tumor necrosis factor and uses thereof |
| US7244616B2 (en) | 2003-06-27 | 2007-07-17 | Bayer Pharmaceuticals Corporation | Use of molecular chaperones for the enhanced production of secreted, recombinant proteins in mammalian cells |
| US7632921B2 (en) | 2004-11-12 | 2009-12-15 | Bayer Healthcare Llc | Site-directed modification of FVIII |
| WO2008149147A2 (en) | 2007-06-06 | 2008-12-11 | Domantis Limited | Polypeptides, antibody variable domains and antagonists |
| ES2517603T3 (es) | 2008-04-07 | 2014-11-03 | Bayer Healthcare, Llc | Procedimientos de producción recombinante de glucoproteínas |
| KR101005967B1 (ko) | 2008-04-12 | 2011-01-05 | (주)셀트리온 | 우수한 재조합 단백질을 생산하기 위한 인간 숙주 세포 |
| US9090948B2 (en) * | 2008-09-30 | 2015-07-28 | Abbott Molecular Inc. | Primers and probes for detecting human papillomavirus and human beta globin sequences in test samples |
| US8778677B2 (en) * | 2009-06-10 | 2014-07-15 | BTS Research International PTY. Ltd. | Methods of generating hybrid/chimeric cells, and uses thereof |
| US11649449B2 (en) | 2015-09-03 | 2023-05-16 | CHO Plus, Inc. | Hybrid cell lines for high level production of a target protein |
| WO2018017144A1 (en) | 2016-07-19 | 2018-01-25 | Brandeis University | Compositions and methods for identifying rna binding polypeptide targets |
| AU2019270342A1 (en) | 2018-05-16 | 2020-10-29 | Morphosys Ag | Antibodies targeting glycoprotein VI |
| SG11202108761YA (en) | 2019-03-14 | 2021-09-29 | Morphosys Ag | Antibodies targeting c5ar |
| CN114096659A (zh) * | 2019-06-10 | 2022-02-25 | Cho普拉斯公司 | 用于基于蛋白质的药物的高水平生产的细胞系 |
| EP4217391A1 (en) | 2020-09-24 | 2023-08-02 | MorphoSys AG | Novel human antibodies binding to human cd3 epsilon |
| CN113604425B (zh) * | 2021-06-22 | 2022-10-18 | 中山康天晟合生物技术有限公司 | 一种适应无血清培养基环境的wayne293 lvpro细胞及其应用 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6045849B2 (ja) * | 1980-08-25 | 1985-10-12 | 林原 健 | ヒトエリトロポエチンの製造方法 |
| JPS6030654B2 (ja) * | 1980-12-31 | 1985-07-17 | 株式会社林原生物化学研究所 | ヒトコロニ−刺激因子の製造方法 |
| US6136599A (en) * | 1998-12-10 | 2000-10-24 | Bayer Corporation | Human hybrid host cell for mammalian gene expression |
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1998
- 1998-12-10 US US09/209,920 patent/US6136599A/en not_active Expired - Lifetime
-
1999
- 1999-08-12 UA UA2001074776A patent/UA77642C2/uk unknown
- 1999-12-08 PL PL349625A patent/PL205549B1/pl unknown
- 1999-12-08 AT AT99963067T patent/ATE306547T1/de active
- 1999-12-08 CN CNB998142999A patent/CN1230532C/zh not_active Expired - Lifetime
- 1999-12-08 CA CA2353698A patent/CA2353698C/en not_active Expired - Lifetime
- 1999-12-08 HU HU0104681A patent/HU229775B1/hu unknown
- 1999-12-08 RU RU2001119161/13A patent/RU2228355C2/ru active
- 1999-12-08 EP EP99963067A patent/EP1137768B1/en not_active Expired - Lifetime
- 1999-12-08 KR KR1020017007224A patent/KR100627753B1/ko not_active Expired - Lifetime
- 1999-12-08 TR TR2001/01642T patent/TR200101642T2/xx unknown
- 1999-12-08 SK SK791-2001A patent/SK287888B6/sk not_active IP Right Cessation
- 1999-12-08 DK DK99963067T patent/DK1137768T3/da active
- 1999-12-08 JP JP2000586896A patent/JP4332300B2/ja not_active Expired - Lifetime
- 1999-12-08 AU AU19383/00A patent/AU757188B2/en not_active Expired
- 1999-12-08 BR BRPI9916095A patent/BRPI9916095B8/pt not_active IP Right Cessation
- 1999-12-08 CZ CZ20012023A patent/CZ302677B6/cs not_active IP Right Cessation
- 1999-12-08 IL IL14335199A patent/IL143351A0/xx unknown
- 1999-12-08 ID IDW00200101235A patent/ID30448A/id unknown
- 1999-12-08 DE DE69927707T patent/DE69927707T2/de not_active Expired - Lifetime
- 1999-12-08 SI SI9920096A patent/SI20693B/sl active Search and Examination
- 1999-12-08 WO PCT/US1999/029332 patent/WO2000034462A1/en not_active Ceased
- 1999-12-08 ES ES99963067T patent/ES2249929T3/es not_active Expired - Lifetime
- 1999-12-08 RO ROA200100648A patent/RO121563B1/ro unknown
-
2001
- 2001-05-24 IL IL143351A patent/IL143351A/en not_active IP Right Cessation
- 2001-06-06 BG BG105566A patent/BG65263B1/bg unknown
- 2001-06-07 NO NO20012806A patent/NO330548B1/no not_active IP Right Cessation
- 2001-06-07 CU CU20010130A patent/CU23382A3/es not_active IP Right Cessation
-
2002
- 2002-08-26 CU CU20020182A patent/CU23238A1/es unknown
-
2009
- 2009-03-06 JP JP2009053279A patent/JP5508740B2/ja not_active Expired - Lifetime
-
2014
- 2014-02-24 JP JP2014032748A patent/JP5826311B2/ja not_active Expired - Lifetime
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| IF | Valid on the event date | ||
| OU02 | Decision according to article 73(2) ipa 1992, publication of decision on partial fulfilment of the invention and change of patent claims |
Effective date: 20081017 |