NZ512235A - Human hybrid host cell for mammalian gene expression - Google Patents
Human hybrid host cell for mammalian gene expressionInfo
- Publication number
- NZ512235A NZ512235A NZ512235A NZ51223599A NZ512235A NZ 512235 A NZ512235 A NZ 512235A NZ 512235 A NZ512235 A NZ 512235A NZ 51223599 A NZ51223599 A NZ 51223599A NZ 512235 A NZ512235 A NZ 512235A
- Authority
- NZ
- New Zealand
- Prior art keywords
- cells
- human
- recombinant
- gene expression
- mammalian gene
- Prior art date
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Human/human hybrid cells were made via fusion of human embryonic kidney cells (293S) and modified Burkitt's lymphoma cells (2B8). The fusion cells are useful as host cells for the recombinant expression of mammalian genes. The advantages of using these hybrid clones of human kidney- and B-cells, called HKBs, for mammalian gene expression, include (i) the cells are negative for immunoglobulin expression, (ii) the cells grow easily in plasma protein- free medium (with or without the addition of recombinant insulin) as suspension cultures in a shake flask or in a fermenter (iii) the cells are very susceptible for transfection of DNA, and (iv) the cells secrete high levels of heterologous recombinant proteins, such as recombinant monoclonal antibodies, soluble ICAM-1, rIL-4, and rFVIII
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
NZ512235A NZ512235A (en) | 1999-12-08 | 1999-12-08 | Human hybrid host cell for mammalian gene expression |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
NZ512235A NZ512235A (en) | 1999-12-08 | 1999-12-08 | Human hybrid host cell for mammalian gene expression |
PCT/US1999/029332 WO2000034462A1 (en) | 1998-12-10 | 1999-12-08 | Human hybrid host cell for mammalian gene expression |
Publications (1)
Publication Number | Publication Date |
---|---|
NZ512235A true NZ512235A (en) | 2004-03-26 |
Family
ID=32041059
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
NZ512235A NZ512235A (en) | 1999-12-08 | 1999-12-08 | Human hybrid host cell for mammalian gene expression |
Country Status (1)
Country | Link |
---|---|
NZ (1) | NZ512235A (en) |
-
1999
- 1999-12-08 NZ NZ512235A patent/NZ512235A/en not_active IP Right Cessation
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Legal Events
Date | Code | Title | Description |
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PSEA | Patent sealed | ||
RENW | Renewal (renewal fees accepted) | ||
RENW | Renewal (renewal fees accepted) | ||
RENW | Renewal (renewal fees accepted) | ||
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Free format text: PATENT RENEWED FOR 7 YEARS UNTIL 08 DEC 2019 BY COMPUTER PACKAGES INC. Effective date: 20121227 |
|
EXPY | Patent expired |