CU23382A3 - Metodo de producir celulas utiles para la expresion de una proteina heterologa - Google Patents
Metodo de producir celulas utiles para la expresion de una proteina heterologaInfo
- Publication number
- CU23382A3 CU23382A3 CU20010130A CU20010130A CU23382A3 CU 23382 A3 CU23382 A3 CU 23382A3 CU 20010130 A CU20010130 A CU 20010130A CU 20010130 A CU20010130 A CU 20010130A CU 23382 A3 CU23382 A3 CU 23382A3
- Authority
- CU
- Cuba
- Prior art keywords
- cells
- expression
- recombinant
- human
- fusion
- Prior art date
Links
- 230000014509 gene expression Effects 0.000 title abstract 4
- 108090000623 proteins and genes Proteins 0.000 title abstract 2
- 102000004169 proteins and genes Human genes 0.000 title abstract 2
- 210000004027 cell Anatomy 0.000 abstract 7
- 230000004927 fusion Effects 0.000 abstract 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 abstract 2
- 210000003292 kidney cell Anatomy 0.000 abstract 2
- 208000011691 Burkitt lymphomas Diseases 0.000 abstract 1
- 102100026735 Coagulation factor VIII Human genes 0.000 abstract 1
- 101000911390 Homo sapiens Coagulation factor VIII Proteins 0.000 abstract 1
- 108060003951 Immunoglobulin Proteins 0.000 abstract 1
- 102000004877 Insulin Human genes 0.000 abstract 1
- 108090001061 Insulin Proteins 0.000 abstract 1
- 108010064593 Intercellular Adhesion Molecule-1 Proteins 0.000 abstract 1
- 102100037877 Intercellular adhesion molecule 1 Human genes 0.000 abstract 1
- 108090000978 Interleukin-4 Proteins 0.000 abstract 1
- 210000003719 b-lymphocyte Anatomy 0.000 abstract 1
- 210000004754 hybrid cell Anatomy 0.000 abstract 1
- 102000018358 immunoglobulin Human genes 0.000 abstract 1
- 229940125396 insulin Drugs 0.000 abstract 1
- 238000004114 suspension culture Methods 0.000 abstract 1
- 238000001890 transfection Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/02—Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
- C12N5/12—Fused cells, e.g. hybridomas
- C12N5/16—Animal cells
- C12N5/163—Animal cells one of the fusion partners being a B or a T lymphocyte
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/02—Cells for production
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
Las celulas hibridos humana fueron hechas vias fusion de celulas de rinon embrionico humano (293S) y celulas de linfoma de Burkitt modificadas (2B8). Las celulas fusion son ·tiles como celulas huespedes para la expresion recombinante de gene de mamiferos. Las ventajas del uso de estos clones hibridos de rinon humano - y celulas B, llamadas HKBs, para expresion de gen de mamifero, incluye (i) las celulas son negativas para la expresion de inmunoglobulina, (ii) las celulas crecen facilmente en plasma de medio libre de proteina (con o sin adicion de insulina recombinante) como cultura de suspension en un frasco de batir o en un fermentador (iii) las celulas son muy susceptibles para la transfeccion de ADN y (iv) las celulas segregan altos niveles de proteinas recombinantes heterologas, tales como anticuerpos monoclonales recombinantes, ICAM-1 soluble r IL-4 y r FVIII. .
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/209,920 US6136599A (en) | 1998-12-10 | 1998-12-10 | Human hybrid host cell for mammalian gene expression |
Publications (1)
Publication Number | Publication Date |
---|---|
CU23382A3 true CU23382A3 (es) | 2009-06-25 |
Family
ID=22780882
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CU20010130A CU23382A3 (es) | 1998-12-10 | 2001-06-07 | Metodo de producir celulas utiles para la expresion de una proteina heterologa |
CU20020182A CU23238A1 (es) | 1998-12-10 | 2002-08-26 | CéLULAS HUéSPED PARA LA EXPRESION DE UNA PROTEINA HETEROLOGA |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CU20020182A CU23238A1 (es) | 1998-12-10 | 2002-08-26 | CéLULAS HUéSPED PARA LA EXPRESION DE UNA PROTEINA HETEROLOGA |
Country Status (27)
Country | Link |
---|---|
US (1) | US6136599A (es) |
EP (1) | EP1137768B1 (es) |
JP (3) | JP4332300B2 (es) |
KR (1) | KR100627753B1 (es) |
CN (1) | CN1230532C (es) |
AT (1) | ATE306547T1 (es) |
AU (1) | AU757188B2 (es) |
BG (1) | BG65263B1 (es) |
BR (1) | BRPI9916095B8 (es) |
CA (1) | CA2353698C (es) |
CU (2) | CU23382A3 (es) |
CZ (1) | CZ302677B6 (es) |
DE (1) | DE69927707T2 (es) |
DK (1) | DK1137768T3 (es) |
ES (1) | ES2249929T3 (es) |
HU (1) | HU229775B1 (es) |
ID (1) | ID30448A (es) |
IL (2) | IL143351A0 (es) |
NO (1) | NO330548B1 (es) |
PL (1) | PL205549B1 (es) |
RO (1) | RO121563B1 (es) |
RU (1) | RU2228355C2 (es) |
SI (1) | SI20693B (es) |
SK (1) | SK287888B6 (es) |
TR (1) | TR200101642T2 (es) |
UA (1) | UA77642C2 (es) |
WO (1) | WO2000034462A1 (es) |
Families Citing this family (23)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6136599A (en) * | 1998-12-10 | 2000-10-24 | Bayer Corporation | Human hybrid host cell for mammalian gene expression |
US6358703B1 (en) * | 1998-12-10 | 2002-03-19 | Bayer Corporation | Expression system for factor VIII |
US6348192B1 (en) * | 1999-05-11 | 2002-02-19 | Bayer Corporation | Interleukin-2 mutein expressed from mammalian cells |
US20040235715A1 (en) * | 1999-11-12 | 2004-11-25 | Bayer Corporation | Method of producing glycosylated bikunin |
US6569657B1 (en) * | 2000-06-27 | 2003-05-27 | Millennium Pharmaceuticals, Inc. | 32140, a novel human aldehyde dehydrogenase and uses therefor |
US7094587B2 (en) * | 2000-06-27 | 2006-08-22 | Millennium Pharmaceuticals, Inc. | 16002 Molecules and uses therefor |
US7723102B2 (en) * | 2000-09-28 | 2010-05-25 | Bayer Corporation | Enhanced transfection system |
MY139948A (en) * | 2000-09-28 | 2009-11-30 | Bayer Corp | Enhanced transfection system |
US20060073141A1 (en) * | 2001-06-28 | 2006-04-06 | Domantis Limited | Compositions and methods for treating inflammatory disorders |
KR100496861B1 (ko) | 2002-09-26 | 2005-06-22 | 삼성전자주식회사 | 하나의 핸들러에 2개 이상의 테스트 보드를 갖는 테스트장비 및 그 테스트 방법 |
RU2377253C2 (ru) | 2002-12-02 | 2009-12-27 | Амген Фремонт,Инк. | Антитела, специфичные к фактору некроза опухолей, и их применение |
US7244616B2 (en) | 2003-06-27 | 2007-07-17 | Bayer Pharmaceuticals Corporation | Use of molecular chaperones for the enhanced production of secreted, recombinant proteins in mammalian cells |
EP3153181A1 (en) | 2004-11-12 | 2017-04-12 | Bayer HealthCare LLC | Site-directed modification of fviii bdd |
US8877186B2 (en) | 2007-06-06 | 2014-11-04 | Domantis Limited | Polypeptides, antibody variable domains and antagonists |
ES2517603T3 (es) | 2008-04-07 | 2014-11-03 | Bayer Healthcare, Llc | Procedimientos de producción recombinante de glucoproteínas |
KR101005967B1 (ko) * | 2008-04-12 | 2011-01-05 | (주)셀트리온 | 우수한 재조합 단백질을 생산하기 위한 인간 숙주 세포 |
US9090948B2 (en) * | 2008-09-30 | 2015-07-28 | Abbott Molecular Inc. | Primers and probes for detecting human papillomavirus and human beta globin sequences in test samples |
CN102612556A (zh) * | 2009-06-10 | 2012-07-25 | Bts研究国际股份有限公司 | 制备杂交细胞/嵌合细胞的方法及其应用 |
US11401514B2 (en) | 2016-07-19 | 2022-08-02 | Brandeis University | Compositions and methods for identifying RNA binding polypeptide targets |
CN112118869A (zh) | 2018-05-16 | 2020-12-22 | 莫佛塞斯公司 | 靶向糖蛋白vi的抗体 |
TW202041535A (zh) | 2019-03-14 | 2020-11-16 | 德商莫菲西斯公司 | 靶向C5aR的抗體 |
KR20230074192A (ko) | 2020-09-24 | 2023-05-26 | 모르포시스 아게 | 인간 cd3 엡실론에 결합하는 신규의 인간 항체 |
CN113604425B (zh) * | 2021-06-22 | 2022-10-18 | 中山康天晟合生物技术有限公司 | 一种适应无血清培养基环境的wayne293 lvpro细胞及其应用 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS6045849B2 (ja) * | 1980-08-25 | 1985-10-12 | 林原 健 | ヒトエリトロポエチンの製造方法 |
US6136599A (en) * | 1998-12-10 | 2000-10-24 | Bayer Corporation | Human hybrid host cell for mammalian gene expression |
-
1998
- 1998-12-10 US US09/209,920 patent/US6136599A/en not_active Expired - Lifetime
-
1999
- 1999-08-12 UA UA2001074776A patent/UA77642C2/uk unknown
- 1999-12-08 CN CNB998142999A patent/CN1230532C/zh not_active Expired - Lifetime
- 1999-12-08 BR BRPI9916095A patent/BRPI9916095B8/pt not_active IP Right Cessation
- 1999-12-08 SK SK791-2001A patent/SK287888B6/sk not_active IP Right Cessation
- 1999-12-08 KR KR1020017007224A patent/KR100627753B1/ko active IP Right Grant
- 1999-12-08 SI SI9920096A patent/SI20693B/sl active Search and Examination
- 1999-12-08 AT AT99963067T patent/ATE306547T1/de active
- 1999-12-08 TR TR2001/01642T patent/TR200101642T2/xx unknown
- 1999-12-08 DE DE69927707T patent/DE69927707T2/de not_active Expired - Lifetime
- 1999-12-08 RO ROA200100648A patent/RO121563B1/ro unknown
- 1999-12-08 AU AU19383/00A patent/AU757188B2/en not_active Expired
- 1999-12-08 DK DK99963067T patent/DK1137768T3/da active
- 1999-12-08 CA CA2353698A patent/CA2353698C/en not_active Expired - Lifetime
- 1999-12-08 RU RU2001119161/13A patent/RU2228355C2/ru active
- 1999-12-08 ID IDW00200101235A patent/ID30448A/id unknown
- 1999-12-08 IL IL14335199A patent/IL143351A0/xx unknown
- 1999-12-08 WO PCT/US1999/029332 patent/WO2000034462A1/en active IP Right Grant
- 1999-12-08 EP EP99963067A patent/EP1137768B1/en not_active Expired - Lifetime
- 1999-12-08 PL PL349625A patent/PL205549B1/pl unknown
- 1999-12-08 ES ES99963067T patent/ES2249929T3/es not_active Expired - Lifetime
- 1999-12-08 CZ CZ20012023A patent/CZ302677B6/cs not_active IP Right Cessation
- 1999-12-08 JP JP2000586896A patent/JP4332300B2/ja not_active Expired - Lifetime
- 1999-12-08 HU HU0104681A patent/HU229775B1/hu unknown
-
2001
- 2001-05-24 IL IL143351A patent/IL143351A/en not_active IP Right Cessation
- 2001-06-06 BG BG105566A patent/BG65263B1/bg unknown
- 2001-06-07 NO NO20012806A patent/NO330548B1/no not_active IP Right Cessation
- 2001-06-07 CU CU20010130A patent/CU23382A3/es not_active IP Right Cessation
-
2002
- 2002-08-26 CU CU20020182A patent/CU23238A1/es unknown
-
2009
- 2009-03-06 JP JP2009053279A patent/JP5508740B2/ja not_active Expired - Lifetime
-
2014
- 2014-02-24 JP JP2014032748A patent/JP5826311B2/ja not_active Expired - Lifetime
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