BR9916095A - Célula hospedeira hìbrida humana para expressão de genes de mamìferos - Google Patents
Célula hospedeira hìbrida humana para expressão de genes de mamìferosInfo
- Publication number
- BR9916095A BR9916095A BR9916095-1A BR9916095A BR9916095A BR 9916095 A BR9916095 A BR 9916095A BR 9916095 A BR9916095 A BR 9916095A BR 9916095 A BR9916095 A BR 9916095A
- Authority
- BR
- Brazil
- Prior art keywords
- cells
- human
- expression
- hybrid
- recombinant
- Prior art date
Links
- 230000014509 gene expression Effects 0.000 title abstract 4
- 108090000623 proteins and genes Proteins 0.000 title abstract 3
- 210000004027 cell Anatomy 0.000 abstract 8
- 230000004927 fusion Effects 0.000 abstract 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 abstract 2
- 108060003951 Immunoglobulin Proteins 0.000 abstract 1
- 102000004877 Insulin Human genes 0.000 abstract 1
- 108090001061 Insulin Proteins 0.000 abstract 1
- 108010064593 Intercellular Adhesion Molecule-1 Proteins 0.000 abstract 1
- 102100037877 Intercellular adhesion molecule 1 Human genes 0.000 abstract 1
- 206010025323 Lymphomas Diseases 0.000 abstract 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract 1
- 210000003719 b-lymphocyte Anatomy 0.000 abstract 1
- 210000005260 human cell Anatomy 0.000 abstract 1
- 102000018358 immunoglobulin Human genes 0.000 abstract 1
- 229940125396 insulin Drugs 0.000 abstract 1
- 210000003734 kidney Anatomy 0.000 abstract 1
- 210000003292 kidney cell Anatomy 0.000 abstract 1
- 238000003259 recombinant expression Methods 0.000 abstract 1
- 238000004114 suspension culture Methods 0.000 abstract 1
- 238000001890 transfection Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/02—Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
- C12N5/12—Fused cells, e.g. hybridomas
- C12N5/16—Animal cells
- C12N5/163—Animal cells one of the fusion partners being a B or a T lymphocyte
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/02—Cells for production
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
Patente de Invenção: <B>"CéLULA HOSPEDEIRA HìBRIDA HUMANA PARA EXPRESSãO DE GENES DE MAMìFEROS"<D>. Células híbridas /humana/humana são feitas via fusão de células de rim embriónico humano (293S) e células de linfoma de Brukitt modificada (2B8). AS células de fusão são utilizáveis como células hospedeiras para a expressão recombinante de genes de mamíferos. As vantagens de usar estes clones híbridos de células B e de rim humano, chamados HKBs, para expressão de genes de mamíferos, incluem (i) as células são negativas para expressão de imunoglobulina, (ii) as células crescem facilmente em meio isento de protéina de plasma, (com ou sem a adição de insulina recombinante), como culturas de suspensão em um frasco de agitação ou em um fermentador (iii) as células são muito susceptíveis a transfecção de DNA, e (iv) as células secretam altos níveis de proteínas recombinantes heterólogas, como anticorpos monoclonais recombinantes, ICAM-1 solúvel, rIL-4 e rFVIII.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/209,920 US6136599A (en) | 1998-12-10 | 1998-12-10 | Human hybrid host cell for mammalian gene expression |
| PCT/US1999/029332 WO2000034462A1 (en) | 1998-12-10 | 1999-12-08 | Human hybrid host cell for mammalian gene expression |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| BR9916095A true BR9916095A (pt) | 2001-09-04 |
| BRPI9916095B1 BRPI9916095B1 (pt) | 2015-10-13 |
| BRPI9916095B8 BRPI9916095B8 (pt) | 2021-05-25 |
Family
ID=22780882
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI9916095A BRPI9916095B8 (pt) | 1998-12-10 | 1999-12-08 | célula e linhagem de célula expressando uma proteína heteróloga |
Country Status (27)
| Country | Link |
|---|---|
| US (1) | US6136599A (pt) |
| EP (1) | EP1137768B1 (pt) |
| JP (3) | JP4332300B2 (pt) |
| KR (1) | KR100627753B1 (pt) |
| CN (1) | CN1230532C (pt) |
| AT (1) | ATE306547T1 (pt) |
| AU (1) | AU757188B2 (pt) |
| BG (1) | BG65263B1 (pt) |
| BR (1) | BRPI9916095B8 (pt) |
| CA (1) | CA2353698C (pt) |
| CU (2) | CU23382A3 (pt) |
| CZ (1) | CZ302677B6 (pt) |
| DE (1) | DE69927707T2 (pt) |
| DK (1) | DK1137768T3 (pt) |
| ES (1) | ES2249929T3 (pt) |
| HU (1) | HU229775B1 (pt) |
| ID (1) | ID30448A (pt) |
| IL (2) | IL143351A0 (pt) |
| NO (1) | NO330548B1 (pt) |
| PL (1) | PL205549B1 (pt) |
| RO (1) | RO121563B1 (pt) |
| RU (1) | RU2228355C2 (pt) |
| SI (1) | SI20693B (pt) |
| SK (1) | SK287888B6 (pt) |
| TR (1) | TR200101642T2 (pt) |
| UA (1) | UA77642C2 (pt) |
| WO (1) | WO2000034462A1 (pt) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6136599A (en) * | 1998-12-10 | 2000-10-24 | Bayer Corporation | Human hybrid host cell for mammalian gene expression |
| US6358703B1 (en) | 1998-12-10 | 2002-03-19 | Bayer Corporation | Expression system for factor VIII |
| US6348192B1 (en) * | 1999-05-11 | 2002-02-19 | Bayer Corporation | Interleukin-2 mutein expressed from mammalian cells |
| US20040235715A1 (en) * | 1999-11-12 | 2004-11-25 | Bayer Corporation | Method of producing glycosylated bikunin |
| US7094587B2 (en) * | 2000-06-27 | 2006-08-22 | Millennium Pharmaceuticals, Inc. | 16002 Molecules and uses therefor |
| US6569657B1 (en) * | 2000-06-27 | 2003-05-27 | Millennium Pharmaceuticals, Inc. | 32140, a novel human aldehyde dehydrogenase and uses therefor |
| MY139948A (en) * | 2000-09-28 | 2009-11-30 | Bayer Corp | Enhanced transfection system |
| US7723102B2 (en) * | 2000-09-28 | 2010-05-25 | Bayer Corporation | Enhanced transfection system |
| US20060073141A1 (en) * | 2001-06-28 | 2006-04-06 | Domantis Limited | Compositions and methods for treating inflammatory disorders |
| KR100496861B1 (ko) | 2002-09-26 | 2005-06-22 | 삼성전자주식회사 | 하나의 핸들러에 2개 이상의 테스트 보드를 갖는 테스트장비 및 그 테스트 방법 |
| DE60333228D1 (de) | 2002-12-02 | 2010-08-12 | Amgen Fremont Inc | Gegen den tumor nekrose faktor gerichtete antikörper und deren verwendungen |
| US7244616B2 (en) | 2003-06-27 | 2007-07-17 | Bayer Pharmaceuticals Corporation | Use of molecular chaperones for the enhanced production of secreted, recombinant proteins in mammalian cells |
| SI3130601T1 (sl) | 2004-11-12 | 2020-11-30 | Bayer Healthcare Llc | Usmerjena modifikacija FVIII |
| BRPI0812398A2 (pt) | 2007-06-06 | 2019-09-24 | Domantis Ltd | domínio variável simples de imunoglobulina anti-vegf, antagonista anti-vegf, domínio variável simples de imunoglobulina resistente à protease, uso do antagonista vegf, método para a dispensação oral ou dispensação de um medicamento ao trato gi de um paciente ou ao pulmão ou tecido pulmonar ou olho de um paciente, dispositivo de dispensação pulmonar, formulação oral, ligando específico duplo, ácido nucleico isolado ou recombinante, vetor, célula hospedeira, método para produzir polipeptídeo, composição farmacêutica, polipeptídeo, e, proteína de fusão |
| WO2009126564A1 (en) | 2008-04-07 | 2009-10-15 | Bayer Healthcare Llc | Methods of recombinant production of glycoproteins |
| KR101005967B1 (ko) | 2008-04-12 | 2011-01-05 | (주)셀트리온 | 우수한 재조합 단백질을 생산하기 위한 인간 숙주 세포 |
| US9090948B2 (en) * | 2008-09-30 | 2015-07-28 | Abbott Molecular Inc. | Primers and probes for detecting human papillomavirus and human beta globin sequences in test samples |
| US8778677B2 (en) * | 2009-06-10 | 2014-07-15 | BTS Research International PTY. Ltd. | Methods of generating hybrid/chimeric cells, and uses thereof |
| EP3487998B1 (en) | 2016-07-19 | 2023-07-12 | Brandeis University | Compositions and methods for identifying rna binding polypeptide targets |
| WO2019219765A1 (en) | 2018-05-16 | 2019-11-21 | Morphosys Ag | Antibodies targeting glycoprotein vi |
| MA55285A (fr) | 2019-03-14 | 2022-01-19 | Morphosys Ag | Anticorps ciblant c5ar |
| KR102648479B1 (ko) * | 2019-06-10 | 2024-03-15 | 초 플러스 인크. | 단백질 기반 의약들 (protein-based pharmaceuticals) 의 고 수준 생산을 위한 세포주들 (cell lines) |
| US20230357398A1 (en) | 2020-09-24 | 2023-11-09 | Morphosys Ag | Novel human antibodies binding to human cd3 epsilon |
| CN113604425B (zh) * | 2021-06-22 | 2022-10-18 | 中山康天晟合生物技术有限公司 | 一种适应无血清培养基环境的wayne293 lvpro细胞及其应用 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6045849B2 (ja) * | 1980-08-25 | 1985-10-12 | 林原 健 | ヒトエリトロポエチンの製造方法 |
| US6136599A (en) * | 1998-12-10 | 2000-10-24 | Bayer Corporation | Human hybrid host cell for mammalian gene expression |
-
1998
- 1998-12-10 US US09/209,920 patent/US6136599A/en not_active Expired - Lifetime
-
1999
- 1999-08-12 UA UA2001074776A patent/UA77642C2/uk unknown
- 1999-12-08 SI SI9920096A patent/SI20693B/sl active Search and Examination
- 1999-12-08 ID IDW00200101235A patent/ID30448A/id unknown
- 1999-12-08 JP JP2000586896A patent/JP4332300B2/ja not_active Expired - Lifetime
- 1999-12-08 HU HU0104681A patent/HU229775B1/hu unknown
- 1999-12-08 RO ROA200100648A patent/RO121563B1/ro unknown
- 1999-12-08 PL PL349625A patent/PL205549B1/pl unknown
- 1999-12-08 TR TR2001/01642T patent/TR200101642T2/xx unknown
- 1999-12-08 EP EP99963067A patent/EP1137768B1/en not_active Expired - Lifetime
- 1999-12-08 WO PCT/US1999/029332 patent/WO2000034462A1/en active IP Right Grant
- 1999-12-08 CN CNB998142999A patent/CN1230532C/zh not_active Expired - Lifetime
- 1999-12-08 ES ES99963067T patent/ES2249929T3/es not_active Expired - Lifetime
- 1999-12-08 BR BRPI9916095A patent/BRPI9916095B8/pt not_active IP Right Cessation
- 1999-12-08 DE DE69927707T patent/DE69927707T2/de not_active Expired - Lifetime
- 1999-12-08 AU AU19383/00A patent/AU757188B2/en not_active Expired
- 1999-12-08 SK SK791-2001A patent/SK287888B6/sk not_active IP Right Cessation
- 1999-12-08 KR KR1020017007224A patent/KR100627753B1/ko active IP Right Grant
- 1999-12-08 AT AT99963067T patent/ATE306547T1/de active
- 1999-12-08 RU RU2001119161/13A patent/RU2228355C2/ru active
- 1999-12-08 CZ CZ20012023A patent/CZ302677B6/cs not_active IP Right Cessation
- 1999-12-08 IL IL14335199A patent/IL143351A0/xx unknown
- 1999-12-08 DK DK99963067T patent/DK1137768T3/da active
- 1999-12-08 CA CA2353698A patent/CA2353698C/en not_active Expired - Lifetime
-
2001
- 2001-05-24 IL IL143351A patent/IL143351A/en not_active IP Right Cessation
- 2001-06-06 BG BG105566A patent/BG65263B1/bg unknown
- 2001-06-07 CU CU20010130A patent/CU23382A3/es not_active IP Right Cessation
- 2001-06-07 NO NO20012806A patent/NO330548B1/no not_active IP Right Cessation
-
2002
- 2002-08-26 CU CU20020182A patent/CU23238A1/es unknown
-
2009
- 2009-03-06 JP JP2009053279A patent/JP5508740B2/ja not_active Expired - Lifetime
-
2014
- 2014-02-24 JP JP2014032748A patent/JP5826311B2/ja not_active Expired - Lifetime
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| B07A | Application suspended after technical examination (opinion) [chapter 7.1 patent gazette] | ||
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| B09B | Patent application refused [chapter 9.2 patent gazette] |
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| B12B | Appeal against refusal [chapter 12.2 patent gazette] | ||
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