SI20214B - Proteins having insecticidal activities and method of use - Google Patents

Proteins having insecticidal activities and method of use Download PDF

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Publication number
SI20214B
SI20214B SI9820048A SI9820048A SI20214B SI 20214 B SI20214 B SI 20214B SI 9820048 A SI9820048 A SI 9820048A SI 9820048 A SI9820048 A SI 9820048A SI 20214 B SI20214 B SI 20214B
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SI
Slovenia
Prior art keywords
nucleotide sequence
plant
plant cell
seq
promoter
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SI9820048A
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English (en)
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SI20214A (sl
Inventor
Amy L Cigan
Thomas H Czapla
Patricia Lynne Fallis
Terry E Meyer
Scott A Mundell
Brian T Sabus
Karel R Schubert
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Pioneer Hi Bred Int
Univ Oklahoma
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Publication of SI20214A publication Critical patent/SI20214A/sl
Publication of SI20214B publication Critical patent/SI20214B/sl

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Insects & Arthropods (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Description

(54) PROTEINI Z INSEKTICIDNIMI AKTIVNOSTMI IN POSTOPEK UPORABE

Claims (25)

1. Izolirana nukleotidna sekvenca, ki kodira polipeptid z insekticidno aktivnostjo proti koruznemu koreninskemu črvu, označena s tem, da ima ta molekula sekvenco, izbrano iz skupine, ki obstoji iz:
(a) sekvence, navedene v SEQ ID NO: 1;
(b) nukleotidnih sekvenc, ki se hibridizirajo na sekvenco, navedeno v SEQ ID NO: 1 ob ostrih pogojih, definiranih z izpiralno stringenco 0,3 M NaCl, 0,03 M natrijevega citrata, 0,1 % SDS pri 70 °C;
(c) nukleotidnih sekvenc, ki kodirajo aminokislinsko sekvenco, navedeno v SEQ ID NO: 2; in (d) nukleotidnih sekvenc, ki kodirajo pentin-1 polipeptid iz Pentaclethra.
2. Izolirana nukleotidna sekvenca po zahtevku 1, označena s tem, da kodira protein, naveden v SEQ ID NO:2.
3. Nukleotidna sekvenca po zahtevku 1, označena s tem, daje nukleotidna sekvenca sekvenca, navedena v SEQ ID NO: 1.
4. Nukleotidna sekvenca po zahtevku 1, označena s tem, daje sekvenca sintetska.
5. Nukleotidna sekvenca po zahtevku 1, označena s tem, da smo sekvenco optimirali za ekspresijo v koruzi.
6. Rastlina, ki je stabilno transformirana z ekspresijsko kaseto, ki obsega promotor, ki vodi ekspresijo v rastlinski celici, operabilno vezani na nukleotidno sekvenco po zahtevku 1.
7. Rastlina po zahtevku 6, označena s tem, daje rastlina koruza.
8. Seme rastline po zahtevku 6 ali 7.
9. Rastlina po zahtevku 6, označena s tem, da nukleotidna sekvenca kodira aminokislinsko sekvenco, navedeno v SEQ ID NO:2.
10. Rastlina po zahtevku 6, označena s tem, da je nukleotidna sekvenca sekvenca, navedena v SEQ ID NO: 1.
11. Rastlina po zahtevku 6, označena s tem, da je nukleotidna sekvenca operabilno vezana na koreninsko prednosten promotor.
12. Rastlina po zahtevku 9, označena s tem, da je nukleotidna sekvenca operabilno vezana na koreninsko prednosten promotor.
13. Rastlina po zahtevku 10, označena s tem, daje nukleotidna sekvenca operabilno vezana na koreninsko prednosten promotor.
14. Postopek za zatiranje koruznega koreninskega črva, označen s tem, da obsega: transformiranje rastlinske celice z ekspresijsko kaseto, ki obsega promotor, ki vodi ekspresijo v rastlinski celici, operabilno vezani na nukleotidno sekvenco po zahtevku 1, in regeneriranje transformirane rastline iz rastlinske celice.
15. A. Postopek po zahtevku 14, označen s tem, daje promotor koreninsko prednosten promotor.
16. Postopek po zahtevku 15, označen s tem, da nukleotidna sekvenca kodira aminokislinsko sekvenco, navedeno v SEQ ID NO:2.
17. Postopek po zahtevku 15, označen s tem, daje rastlinska celica iz monokaličnice.
18. Postopek po zahtevku 17, označen s tem, daje monokaličnica koruza.
19. Rastlinska celica, ki smo jo stabilno transformirali z ekspresijsko kaseto, ki obsega promotor, ki vodi ekspresijo v rastlinski celici, operabilno vezani na nukleotidno sekvenco po zahtevku 1.
20. Rastlinska celica po zahtevku 19, označena s tem, da je promotor koreninsko prednosten promotor.
21. Rastlinska celica po zahtevku 20, označena s tem, da nukleotidna sekvenca kodira aminokislinsko sekvenco, navedeno v SEQ ID NO:2.
22. Rastlinska celica po zahtevku 20, označena s tem, da je rastlinska celica iz monokaličnice.
23. Rastlinska celica po zahtevku 22, označena s tem, daje monokaličnica koruza.
24. Vektor, označen s tem, da obsega nukleotidno sekvenco po zahtevku 1.
25. Rastlina ali mikroorganizem, označen s tem, daje bil transformiran z vektorjem po zahtevku 24.
SI9820048A 1997-05-29 1998-05-15 Proteins having insecticidal activities and method of use SI20214B (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US4786497P 1997-05-29 1997-05-29
US09/074,912 US6057491A (en) 1997-05-29 1998-05-08 Protein having insecticidal activities and method of use
PCT/US1998/009995 WO1998054327A1 (en) 1997-05-29 1998-05-15 Proteins having insecticidal activities and method of use

Publications (2)

Publication Number Publication Date
SI20214A SI20214A (sl) 2000-10-31
SI20214B true SI20214B (en) 2001-12-31

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Family Applications (1)

Application Number Title Priority Date Filing Date
SI9820048A SI20214B (en) 1997-05-29 1998-05-15 Proteins having insecticidal activities and method of use

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US (2) US6057491A (sl)
EP (1) EP0986645A1 (sl)
AR (1) AR016059A1 (sl)
AU (1) AU742709B2 (sl)
BR (1) BR9809516A (sl)
CA (1) CA2290776C (sl)
CZ (1) CZ297319B6 (sl)
NZ (1) NZ501218A (sl)
SI (1) SI20214B (sl)
SK (1) SK160099A3 (sl)
WO (1) WO1998054327A1 (sl)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CZ289014B6 (cs) * 1989-09-27 2001-10-17 Dsm N. V. Vyčiątěná a izolovaná sekvence DNA kódující fungální fytázu, konstrukt pro expresi, vektory a transformované hostitelské buňky a způsob produkce fytázy
CA2325567A1 (en) 1998-05-01 1999-11-11 Maxygen, Inc. Optimization of pest resistance genes using dna shuffling
CA2384122C (en) * 1999-09-07 2010-05-04 Meiji Seika Kaisha, Ltd. Cyclic depsipeptide synthetase and gene thereof, and mass production system for cyclic depsipeptide
AU1612901A (en) * 1999-11-15 2001-05-30 Pioneer Hi-Bred International, Inc. Novel proteins having insecticidal activities and method of use
US6657046B1 (en) 2000-01-06 2003-12-02 Monsanto Technology Llc Insect inhibitory lipid acyl hydrolases
EP1254167A2 (en) 2000-01-06 2002-11-06 Monsanto Technology LLC Preparation of deallergenized patatin proteins and patatin permutein proteins
US6664097B2 (en) * 2000-05-23 2003-12-16 North Carolina State University Polynucleotide encoding a Lactobacillus gasseri beta-glucuronidase polypeptide
US6643672B1 (en) * 2000-07-31 2003-11-04 Hewlett-Packard Development Company, Lp. Method and apparatus for asynchronous file writes in a distributed file system
US7282556B2 (en) 2001-05-15 2007-10-16 Emory University Polynucleotides and polypeptides relating to the modulation of SIRPα-CD47
JP4139773B2 (ja) * 2001-08-28 2008-08-27 三菱レイヨン株式会社 新規なアミド加水分解酵素遺伝子
BRPI0507893A (pt) * 2004-02-20 2007-07-24 Pioneer Hi Bred Internacional métodos de criação ou aumento de resistência a insetos em plantas
EP1716230A2 (en) 2004-02-20 2006-11-02 Pioneer Hi-Bred International, Inc. Lipases and methods of use
US20080293087A1 (en) * 2006-10-31 2008-11-27 Makielski Jonathan C Sulfonylurea receptor short forms from mitochondria and uses thereof
CA2751724A1 (en) 2009-02-19 2010-08-26 Pioneer Hi-Bred International, Inc. Blended refuge deployment via manipulation during hybrid seed production
CN103459601A (zh) 2011-02-11 2013-12-18 先锋国际良种公司 具有对抗玉米根虫的活性的合成杀昆虫蛋白
EP2794887A2 (en) 2011-03-30 2014-10-29 Universidad Nacional Autonoma De Mexico Mutant bacillus thuringiensis cry genes and methods of use
CN103502457A (zh) 2011-05-02 2014-01-08 先锋国际良种公司 用于发现编码杀虫剂的新型核酸分子的细菌信使核糖核酸筛选策略
BR112015008504A2 (pt) 2012-10-15 2017-08-22 Pioneer Hi Bred Int Método para aumentar a atividade pesticida, polipeptídeo, molécula de ácido nucleico, cassete de expressão, vetor, método de obtenção de uma planta, método de obtenção de uma célula de planta, composição pesticida, método para proteção de uma planta, método para proteger uma planta, método para aumentar a resistência de uma planta
WO2016000237A1 (en) 2014-07-03 2016-01-07 Pioneer Overseas Corporation Plants having enhanced tolerance to insect pests and related constructs and methods involving insect tolerance genes
US10743535B2 (en) 2017-08-18 2020-08-18 H&K Solutions Llc Insecticide for flight-capable pests

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0587798A4 (en) * 1991-06-07 1995-04-19 Dowelanco INSECTICIDE PROTEINS AND METHOD FOR PROTECTING PLANTS.
US5743477A (en) * 1992-08-27 1998-04-28 Dowelanco Insecticidal proteins and method for plant protection
GB9300124D0 (en) * 1993-01-06 1993-03-03 Zeneca Ltd Bacterial strain
KR960701210A (ko) * 1993-03-12 1996-02-24 제임스 클리프튼 보올딩 제충방법(method of contr0lling insects in plants)
US5824864A (en) * 1995-05-25 1998-10-20 Pioneer Hi-Bred International, Inc. Maize gene and protein for insect control
US5672680A (en) * 1995-11-20 1997-09-30 Pioneer Hi-Bred International, Inc. Pentaclethera macroloba protein having insecticidal properties
US5756661A (en) * 1996-11-15 1998-05-26 Pioneer Hi-Bred International, Inc. Pentaclethra macroloba derived substances having insecticide, agglutination and aminopeptidase inhibition activity

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Publication number Publication date
CZ421199A3 (cs) 2000-04-12
WO1998054327A1 (en) 1998-12-03
AR016059A1 (es) 2001-06-20
WO1998054327A9 (en) 1999-04-01
AU7389198A (en) 1998-12-30
SK160099A3 (en) 2000-06-12
BR9809516A (pt) 2000-06-20
CZ297319B6 (cs) 2006-11-15
NZ501218A (en) 2002-02-01
CA2290776C (en) 2003-10-14
SI20214A (sl) 2000-10-31
US6339144B1 (en) 2002-01-15
US6057491A (en) 2000-05-02
AU742709B2 (en) 2002-01-10
EP0986645A1 (en) 2000-03-22
CA2290776A1 (en) 1998-12-03

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