KR960701210A - 제충방법(method of contr0lling insects in plants) - Google Patents

제충방법(method of contr0lling insects in plants) Download PDF

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Publication number
KR960701210A
KR960701210A KR1019950703878A KR19950703878A KR960701210A KR 960701210 A KR960701210 A KR 960701210A KR 1019950703878 A KR1019950703878 A KR 1019950703878A KR 19950703878 A KR19950703878 A KR 19950703878A KR 960701210 A KR960701210 A KR 960701210A
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South Korea
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plant
code sequence
structural code
fetetin
promoter
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KR1019950703878A
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English (en)
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셰리 마리 브라운
쟌 토마스 그린 플레이트
바바라 건더 이자크
마이클 거라드 제닝스
엘렌 비트리스 리바인
쟌 패트릭 퍼셀
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제임스 클리프튼 보올딩
몬산토 캄파니
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Publication of KR960701210A publication Critical patent/KR960701210A/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/12—Processes for modifying agronomic input traits, e.g. crop yield
    • A01H1/122—Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • A01H1/1245—Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, e.g. pathogen, pest or disease resistance
    • A01H1/127—Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, e.g. pathogen, pest or disease resistance for insect resistance
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/38—Solanaceae [Potato family], e.g. nightshade, tomato, tobacco or chilli pepper
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Botany (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Environmental Sciences (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Pest Control & Pesticides (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Insects & Arthropods (AREA)
  • Mycology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Catching Or Destruction (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

페테틴(Patatins)은 유충의 성장발육을 방해하여, 성숙과 재생을 막으므로써 곤충을 억제한다. 하나이상의 이러한 단백질을 암호하는 유전자들은 식물-기생 미생물들 또는 식물들의 형질전환을 위하여 벡터내로 클론될수 있으며, 이에 의해 곤충만연을 억제하는 방법이 제공된다.

Description

제충방법(METHOD OF CONTROLLING INSECTS IN PLANTS)
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (11)

  1. 곤충에 의한 섭취를 위해 유효량의 살충성 페테틴을 제공하는 것을 포함하는, 식물-섭취 곤충의 만연을 억제하는 방법.
  2. 제1항에 있어서, 상술한 페테틴은, 식물에 적용된 후에 페테틴을 생성하는 식물-기생 미생물에 의하여 제공됨을 특징으로 하는 방법.
  3. 제1항에 있어서, 상술한 페테틴은 식물의 모세포의 사전 유전적 형질전환에 의하여 상술한 식물내에 혼입된 페테틴 유전자의 발현에 의하여 제공됨을 특징으로 하는 방법.
  4. 제3항에 있어서, 상술한 식물은 목화, 옥수수, 토마토 또는 감자인 것을 특징으로 하는 방법.
  5. 다음의 a),b),c)단계를 포함하는, 살충적 유효량의 패테틴을 발현하는, 유전적으로 형질전환되고 곤충에 내성아 있는 식물을 생산하는 방법.
    (i)식물세포내에서 RNA서열이 생산되도록 작용하는 프로모터; (ii)페테틴을 암호하는 구조암호서열: (ii)상술한 식물세포내에서 RNA서열의 3'단말에 폴리아데닐레이트 뉴클레오티드가 첨가되도록 작용하는 3'-비-해독영역을 포함하는 재조합 이중가닥 DNA분자를 식물세포의 게놈내로 삽입하는 단계; 여기서 상술한 프로모터는 상술한 구조암호서열에 대해 이종성이며, 상술한 프로모터는 상술한 구조암호서열과 작동적으로 결합된 다음 상술한 비-해독영역과 작동적으로 결합된다; b)형질전환된 식물세포를 얻는 단계; c)상술한 형질전환된 식물로부터, 살충적으로 유효량의 패테틴을 발현하는 유전학적으로 형질전환된 식물세포를 재생시키는 단계; 여기서 상술한 프로모터는 구조암호서열에 대해 이종성이며, 상술한 식물은 목화, 옥수수, 토마토 및 감자로부터 선택된다.
  6. 제5항에 있어서, 상술한 구조암호서열은 SEQ ID NO:30 또는 SEQ ID NO:31을 포함하는 것을 특징으로 하는 방법.
  7. 제5항에 있어서, 상술한 식물은 옥수수이고, 상술한 구조암호서열은 외떡잎식물에서 향상된 발현을 하도록 합성된 것임을 특징으로 하는 방법.
  8. 제7항에 있어서, 상술한 구조암호서열은 SEQ ID NO:32를 포함하는 것을 특징으로 하는 방법.
  9. 제5항의 방법에 의하여 생산된 식물.
  10. 제9항에 있어서, 게놈내에 하나 이상의 유전자 발현 B.t엔도톡신이 포함된 것을 특징으로 하는 식물.
  11. 제9항의 식물에 의하여 생산된 종자 또는 종자단편.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019950703878A 1993-03-12 1994-03-02 제충방법(method of contr0lling insects in plants) KR960701210A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US3114693A 1993-03-12 1993-03-12
US031,146 1993-03-12
PCT/US1994/002306 WO1994021805A2 (en) 1993-03-12 1994-03-02 Method of controlling insects in plants

Publications (1)

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KR960701210A true KR960701210A (ko) 1996-02-24

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Application Number Title Priority Date Filing Date
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EP (1) EP0688363A1 (ko)
JP (1) JPH08507692A (ko)
KR (1) KR960701210A (ko)
CN (1) CN1119027A (ko)
AU (1) AU677389B2 (ko)
BR (1) BR9406586A (ko)
CA (1) CA2155430A1 (ko)
CZ (1) CZ285629B6 (ko)
HU (1) HUT72479A (ko)
NZ (1) NZ263327A (ko)
PL (1) PL176936B1 (ko)
UA (1) UA27966C2 (ko)
WO (1) WO1994021805A2 (ko)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5824864A (en) * 1995-05-25 1998-10-20 Pioneer Hi-Bred International, Inc. Maize gene and protein for insect control
ES2196340T3 (es) * 1996-06-18 2003-12-16 Unilever Nv Procedimiento de esterificacion enzimatica.
US6080913A (en) * 1996-09-25 2000-06-27 Pioneer Hi-Bred International, Inc. Binary methods of increasing accumulation of essential amino acids in seeds
US6057491A (en) * 1997-05-29 2000-05-02 Borad Of Regents For University Of Oklahoma Protein having insecticidal activities and method of use
WO2001036468A2 (en) * 1999-11-15 2001-05-25 Pioneer Hi-Bred International, Inc. Novel proteins having insecticidal activities and method of use
EP1254167A2 (en) 2000-01-06 2002-11-06 Monsanto Technology LLC Preparation of deallergenized patatin proteins and patatin permutein proteins
FR2807756A1 (fr) * 2000-04-13 2001-10-19 Rhobio Polypeptides pla2 de plantes impliques dans la reaction de defense des plantes, polynucleotides codant ces polypeptides et plantes transformees les contenant

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU638438B2 (en) * 1989-02-24 1993-07-01 Monsanto Technology Llc Synthetic plant genes and method for preparation
DE4013144A1 (de) * 1990-04-20 1991-10-24 Inst Genbiologische Forschung Neue plasmide, enthaltend dna-sequenzen, die veraenderungen der karbohydrat- und proteinkonzentration und der karbohydrat- und proteinzusammensetzung in kartoffelknollen hervorrufen, sowie zellen einer kartoffelpflanze, enthaltend diese plasmide

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Publication number Publication date
AU6403494A (en) 1994-10-11
CZ285629B6 (cs) 1999-10-13
HUT72479A (en) 1996-04-29
CN1119027A (zh) 1996-03-20
WO1994021805A3 (en) 1994-12-22
NZ263327A (en) 1997-01-29
JPH08507692A (ja) 1996-08-20
CA2155430A1 (en) 1994-09-29
WO1994021805A2 (en) 1994-09-29
BR9406586A (pt) 1996-01-02
AU677389B2 (en) 1997-04-24
CZ219495A3 (en) 1996-05-15
HU9502646D0 (en) 1995-11-28
PL176936B1 (pl) 1999-08-31
UA27966C2 (uk) 2000-10-16
PL310599A1 (en) 1995-12-27
EP0688363A1 (en) 1995-12-27

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