SG46627A1 - Recombinant expression vectors and purification methods for thermus thermophilus dna polymerase - Google Patents

Recombinant expression vectors and purification methods for thermus thermophilus dna polymerase

Info

Publication number
SG46627A1
SG46627A1 SG1996006838A SG1996006838A SG46627A1 SG 46627 A1 SG46627 A1 SG 46627A1 SG 1996006838 A SG1996006838 A SG 1996006838A SG 1996006838 A SG1996006838 A SG 1996006838A SG 46627 A1 SG46627 A1 SG 46627A1
Authority
SG
Singapore
Prior art keywords
dna polymerase
thermus thermophilus
expression vectors
recombinant expression
purification methods
Prior art date
Application number
SG1996006838A
Other languages
English (en)
Inventor
David H Gelfand
Frances Lawyer
Susanne Stoffel
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of SG46627A1 publication Critical patent/SG46627A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
SG1996006838A 1989-12-22 1990-12-21 Recombinant expression vectors and purification methods for thermus thermophilus dna polymerase SG46627A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US45596789A 1989-12-22 1989-12-22

Publications (1)

Publication Number Publication Date
SG46627A1 true SG46627A1 (en) 1998-02-20

Family

ID=23810915

Family Applications (1)

Application Number Title Priority Date Filing Date
SG1996006838A SG46627A1 (en) 1989-12-22 1990-12-21 Recombinant expression vectors and purification methods for thermus thermophilus dna polymerase

Country Status (10)

Country Link
EP (1) EP0506825B1 (ja)
JP (2) JP2774192B2 (ja)
AT (1) ATE169337T1 (ja)
AU (2) AU646430B2 (ja)
CA (1) CA2071196C (ja)
DE (1) DE69032543T2 (ja)
DK (1) DK0506825T3 (ja)
ES (1) ES2121777T3 (ja)
SG (1) SG46627A1 (ja)
WO (1) WO1991009950A1 (ja)

Families Citing this family (79)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5466591A (en) * 1986-08-22 1995-11-14 Hoffmann-La Roche Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US5795762A (en) * 1986-08-22 1998-08-18 Roche Molecular Systems, Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
CA2090614C (en) * 1990-09-28 2001-12-11 David H. Gelfand 5' to 3' exonuclease mutations of thermostable dna polymerases
EP1233061A3 (en) * 1991-06-03 2002-09-04 Takara Shuzo Co., Ltd. A method for cloning of a gene for pol I type DNA polymerase
US5994069A (en) * 1996-01-24 1999-11-30 Third Wave Technologies, Inc. Detection of nucleic acids by multiple sequential invasive cleavages
US5846717A (en) * 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US6872816B1 (en) 1996-01-24 2005-03-29 Third Wave Technologies, Inc. Nucleic acid detection kits
US6372424B1 (en) 1995-08-30 2002-04-16 Third Wave Technologies, Inc Rapid detection and identification of pathogens
US5541311A (en) * 1992-12-07 1996-07-30 Third Wave Technologies, Inc. Nucleic acid encoding synthesis-deficient thermostable DNA polymerase
US7785852B2 (en) 1992-12-07 2010-08-31 Third Wave Technologies, Inc. Cleavage of nucleic acids
US6673616B1 (en) 1992-12-07 2004-01-06 Third Wave Technologies, Inc. Methods and compositions for characterizing nucleic acids
US5719028A (en) * 1992-12-07 1998-02-17 Third Wave Technologies Inc. Cleavase fragment length polymorphism
US5843654A (en) * 1992-12-07 1998-12-01 Third Wave Technologies, Inc. Rapid detection of mutations in the p53 gene
US5422253A (en) * 1992-12-07 1995-06-06 Wisconsin Alumni Research Foundation Method of site specific nucleic acid cleavage
US5614402A (en) * 1992-12-07 1997-03-25 Third Wave Technologies, Inc. 5' nucleases derived from thermostable DNA polymerase
US5888780A (en) * 1992-12-07 1999-03-30 Third Wave Technologies, Inc. Rapid detection and identification of nucleic acid variants
EP0730642A1 (en) * 1993-11-25 1996-09-11 Pacific Enzymes Limited Improved polymerase
US5648211A (en) * 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
WO1996014417A1 (en) * 1994-11-04 1996-05-17 Molecular Biology Resources, Inc. Dna encoding a thermostable dna polymerase enzyme
WO1996014405A2 (en) * 1994-11-04 1996-05-17 Molecular Biology Resources, Inc. Biologically active fragments of thermus flavus dna polymerase
US6077664A (en) * 1995-06-07 2000-06-20 Promega Corporation Thermophilic DNA polymerases from Thermotoga neapolitana
US6001645A (en) * 1995-06-07 1999-12-14 Promega Corporation Thermophilic DNA polymerases from thermotoga neapolitana
US5773258A (en) * 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
US6090606A (en) * 1996-01-24 2000-07-18 Third Wave Technologies, Inc. Cleavage agents
US7122364B1 (en) 1998-03-24 2006-10-17 Third Wave Technologies, Inc. FEN endonucleases
US7195871B2 (en) 1996-01-24 2007-03-27 Third Wave Technologies, Inc Methods and compositions for detecting target sequences
US6706471B1 (en) 1996-01-24 2004-03-16 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US6875572B2 (en) 1996-01-24 2005-04-05 Third Wave Technologies, Inc. Nucleic acid detection assays
US5985557A (en) 1996-01-24 1999-11-16 Third Wave Technologies, Inc. Invasive cleavage of nucleic acids
US6291164B1 (en) 1996-11-22 2001-09-18 Invitrogen Corporation Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate
ES2375764T3 (es) 1996-11-29 2012-03-06 Third Wave Technologies, Inc. Endonucleasas fen-1, mezclas y procedimientos de escisión.
EP0983365A1 (en) * 1997-04-08 2000-03-08 The Rockefeller University Enzyme derived from thermophilic organisms that functions as a chromosomal replicase, and preparation and uses thereof
US7556958B1 (en) 1997-04-08 2009-07-07 The Rockefeller University Enzyme derived from thermophilic organisms that functions as a chromosomal replicase, and preparation and uses thereof
US6238905B1 (en) 1997-09-12 2001-05-29 University Technology Corporation Thermophilic polymerase III holoenzyme
US8182991B1 (en) 1997-11-26 2012-05-22 Third Wave Technologies, Inc. FEN-1 endonucleases, mixtures and cleavage methods
WO1999047539A1 (en) * 1998-03-18 1999-09-23 Amersham Pharmacia Biotech, Inc. Thermostable dna polymerase from thermoanaerobacter thermohydrosulfuricus
WO1999053074A1 (en) * 1998-04-09 1999-10-21 The Rockfeller University Enzyme derived from thermophilic organisms that functions as a chromosomal replicase, and preparation and uses thereof
EP1086209B1 (en) 1998-06-17 2007-08-22 Amersham Biosciences Corp. Fy7 polymerase
US6773911B1 (en) 1998-11-23 2004-08-10 Amgen Canada Inc. Apoptosis-inducing factor
US7838225B2 (en) 1999-10-29 2010-11-23 Hologic, Inc. Methods for detection of a target nucleic acid by forming a cleavage structure using a reverse transcriptase
US7118860B2 (en) 1999-10-29 2006-10-10 Stratagene California Methods for detection of a target nucleic acid by capture
US7824859B2 (en) 1999-10-29 2010-11-02 Cytyc Corporation Methods for detection of a target nucleic acid by forming a cleavage structure using an RNA polymerase
US6436677B1 (en) 2000-03-02 2002-08-20 Promega Corporation Method of reverse transcription
US6632645B1 (en) 2000-03-02 2003-10-14 Promega Corporation Thermophilic DNA polymerases from Thermoactinomyces vulgaris
US6677146B1 (en) 2000-03-28 2004-01-13 Replidyne, Inc. Thermophilic polymerase III holoenzyme
WO2003048309A2 (en) * 2001-11-30 2003-06-12 Applera Corporation Thermus thermophilus nucleic acid polymerases
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
CN101793826B (zh) 2004-06-07 2016-04-13 先锋生物科技股份有限公司 用于微流体器件的光学透镜系统和方法
EP1934339B1 (en) * 2005-10-06 2011-12-14 Lucigen Corporation Thermostable viral polymerases and methods of use
JP2007104909A (ja) * 2005-10-11 2007-04-26 Tama Tlo Kk 好熱性細菌サーマス・カワライェンシス由来耐熱性ｄｎａポリメラーゼ
WO2008018469A1 (fr) 2006-08-09 2008-02-14 Arkray, Inc. Procédé d'obtention d'un produit d'amplification par PCR et son utilisation
KR101107911B1 (ko) 2006-11-30 2012-01-25 아크레이 가부시키가이샤 Ｕｇｔ1ａ1 유전자 증폭용 프라이머 셋트, 그것을 포함하는ｕｇｔ1ａ1 유전자 증폭용 시약 및 그 용도
KR101119417B1 (ko) 2006-11-30 2012-04-12 아크레이 가부시키가이샤 비만 유전자 증폭용 프라이머 세트, 그것을 포함하는 비만 유전자 증폭용 시약 및 그 용도
EP2078747A4 (en) 2006-11-30 2009-12-09 Arkray Inc PRIMER SET FOR THE REINFORCEMENT OF THE NAT2 GENE, REAGENT FOR THE REINFORCEMENT OF THE NAT2 GENE THEREFOR AND APPLICATION THEREOF
US8404438B2 (en) 2006-11-30 2013-03-26 Arkray, Inc. Probes for detection of SULT1A1 gene, reagent containing the same, and the uses thereof
WO2008066163A1 (fr) 2006-11-30 2008-06-05 Arkray, Inc. Ensemble d'amorces utilisé dans l'amplification du gène cyp2c9, réactif utilisé dans l'amplification du gène cyp2c9, et son utilisation
EP2055789A4 (en) 2006-11-30 2009-12-23 Arkray Inc PRIMER SET FOR THE REINFORCEMENT OF THE CYP2C19 GENE, REAGENT FOR THE REINFORCEMENT OF THE CYP2C19 GENE THEREFOR AND APPLICATION THEREOF
JP5224526B2 (ja) 2007-03-26 2013-07-03 アークレイ株式会社 遺伝子増幅用プライマーセット、それを含む遺伝子増幅用試薬およびその用途
JPWO2009081967A1 (ja) 2007-12-26 2011-05-06 アークレイ株式会社 標的核酸配列の増幅方法およびそれに用いるプローブ
JP5651022B2 (ja) 2008-03-15 2015-01-07 ホロジック，インコーポレーテッド 増幅反応中の核酸分子を分析するための組成物及び方法
CN102076850B (zh) 2008-07-02 2014-05-07 爱科来株式会社 靶核酸序列的扩增方法、使用该方法的突变检测方法、以及用于该方法的试剂
US20110244460A1 (en) 2008-12-16 2011-10-06 Arkray, Inc. Method for detecting controls for nucleic acid amplification and use thereof
CN102597271B (zh) 2009-10-30 2015-05-20 爱科来株式会社 Egfr基因多态性检测用探针及其用途
KR20120068992A (ko) 2009-10-30 2012-06-27 아크레이 가부시키가이샤 Ｍｐｌ 유전자 다형 검출용 프로브 및 그 용도
US20120231463A1 (en) 2009-11-19 2012-09-13 Arkray, Inc. Primer Set for Amplification of MTHFR Gene, MTHFR Gene Amplification Reagent Containing the Same, and Use of the Same
KR101372876B1 (ko) 2009-12-07 2014-03-10 아크레이 가부시키가이샤 질환 관련 유전자의 다형 검출용 프로브 및 그 용도
KR20120078746A (ko) 2009-12-24 2012-07-10 아크레이 가부시키가이샤 ｃ-ｋｉｔ 유전자의 다형 검출용 프로브 및 그 용도
JP5684737B2 (ja) 2010-01-21 2015-03-18 アークレイ株式会社 標的配列の増幅方法、多型検出方法およびそれに用いる試薬
EP3219814B1 (en) 2010-10-29 2023-02-15 ARKRAY, Inc. Probe for detection of polymorphism in egfr gene, amplification primer, and use thereof
JP5860667B2 (ja) 2010-10-29 2016-02-16 アークレイ株式会社 Ｅｇｆｒエクソン２１ｌ８５８ｒ遺伝子多型検出用プライマーセット及びその用途
EP2497835B1 (en) 2011-03-09 2015-08-12 ARKRAY, Inc. Probe for detecting polymorphism in CYP3A gene, method of detecting polymorphism, method of evaluating drug efficacy, and reagent kit for detecting polymorphism
WO2012170907A2 (en) 2011-06-08 2012-12-13 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
FI3461807T3 (fi) 2011-06-08 2023-09-07 Life Technologies Corp Uudenlaisten detergenttien suunnittelu ja kehitys pcr-järjestelmissä käyttöä varten
WO2014127805A1 (en) 2013-02-19 2014-08-28 Daiken Medical Co., Ltd. Diagnosis method of pathogen
WO2014127806A1 (en) 2013-02-19 2014-08-28 Daiken Medical Co., Ltd. Dna amplifying kit
WO2014127804A1 (en) 2013-02-19 2014-08-28 Daiken Medical Co., Ltd. Pathogen diagnosis system
JP6742238B2 (ja) 2013-10-25 2020-08-19 ライフ テクノロジーズ コーポレーション Ｐｃｒシステムにおいて使用するための新規化合物及びその用途
EP2990493B1 (en) 2014-09-01 2017-05-24 ARKRAY, Inc. Primer reagent for amplifying alk fusion gene, alk fusion gene amplification reagent kit including the same, and alk fusion gene amplification method and alk fusion gene analysis method using the same
JP6490990B2 (ja) 2015-03-05 2019-03-27 アークレイ株式会社 Ｃｙｐ２ｃ１９遺伝子増幅用プライマーセット、ｃｙｐ２ｃ１９遺伝子増幅用キット、遺伝子増幅方法、多型解析方法、及び薬効判定方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1338457C (en) * 1986-08-22 1996-07-16 Henry A. Erlich Purified thermostable enzyme
AU632857C (en) * 1986-08-22 2003-10-09 F. Hoffmann-La Roche Ag Purified thermostable enzyme
IL88923A (en) * 1988-01-12 1995-07-31 Hoffmann La Roche Gene encoding a thermostable dna polymerase from thermus aquaticus said dna polymerase and its purification
JP2531246B2 (ja) * 1988-08-26 1996-09-04 東洋紡績株式会社 耐熱性ｄｎａポリメラ―ゼおよびその製法
AU627809B2 (en) * 1988-12-16 1992-09-03 Abbott Laboratories Isolating thermostable enzymes from engineered mesophiles

Also Published As

Publication number Publication date
JPH09224682A (ja) 1997-09-02
AU646430B2 (en) 1994-02-24
AU681387B2 (en) 1997-08-28
DK0506825T3 (da) 1999-05-03
ES2121777T3 (es) 1998-12-16
EP0506825A1 (en) 1992-10-07
DE69032543D1 (de) 1998-09-10
CA2071196C (en) 2002-04-23
ATE169337T1 (de) 1998-08-15
EP0506825B1 (en) 1998-08-05
AU7176491A (en) 1991-07-24
JPH05504887A (ja) 1993-07-29
JP2790448B2 (ja) 1998-08-27
CA2071196A1 (en) 1991-06-23
AU6329694A (en) 1994-09-01
JP2774192B2 (ja) 1998-07-09
DE69032543T2 (de) 1999-04-15
WO1991009950A1 (en) 1991-07-11

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