ES2121777T3 - Vectores de expresion recombinantes y metodos de purificacion de la dna polimerasa de thermus thermophilus. - Google Patents

Vectores de expresion recombinantes y metodos de purificacion de la dna polimerasa de thermus thermophilus.

Info

Publication number
ES2121777T3
ES2121777T3 ES91902036T ES91902036T ES2121777T3 ES 2121777 T3 ES2121777 T3 ES 2121777T3 ES 91902036 T ES91902036 T ES 91902036T ES 91902036 T ES91902036 T ES 91902036T ES 2121777 T3 ES2121777 T3 ES 2121777T3
Authority
ES
Spain
Prior art keywords
dna polymerase
purification
methods
thermus thermophilus
expression vectors
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91902036T
Other languages
English (en)
Inventor
David H Gelfand
Frances C Lawyer
Susanne Stoffel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Application granted granted Critical
Publication of ES2121777T3 publication Critical patent/ES2121777T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

VECTORES DE EXPRESION RECOMBINANTES Y METODOS DE PURIFICACION DE LA POLIMERASA DEL ADN DEL THERMUS THERMOPHILUS SECUENCIAS DE ADN RECOMBINANTES QUE CODIFICAN LA ACTIVIDAD DE LA POLIMERASA I DEL ADN DEL THERMUS THERMOPHILUS QUE SE PUEDEN UTILIZAR PARA FORMAR VECTORES RECOMBINANTES Y CELULAS HUESPEDES TRANSFORMADAS PARA LA PRODUCCION DE LA ACTIVIDAD. LA POLIMERASA I DEL ADN DEL T. THERMOPHILUS ES UNA PROTEINA ~94 KDA ESPECIALMENTE UTIL EN EL PROCESO DE AMPLIACION DE ADN CONOCIDO COMO LA REACCION EN CADENA DE LA POLIMERASA.
ES91902036T 1989-12-22 1990-12-21 Vectores de expresion recombinantes y metodos de purificacion de la dna polimerasa de thermus thermophilus. Expired - Lifetime ES2121777T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US45596789A 1989-12-22 1989-12-22

Publications (1)

Publication Number Publication Date
ES2121777T3 true ES2121777T3 (es) 1998-12-16

Family

ID=23810915

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91902036T Expired - Lifetime ES2121777T3 (es) 1989-12-22 1990-12-21 Vectores de expresion recombinantes y metodos de purificacion de la dna polimerasa de thermus thermophilus.

Country Status (10)

Country Link
EP (1) EP0506825B1 (es)
JP (2) JP2774192B2 (es)
AT (1) ATE169337T1 (es)
AU (2) AU646430B2 (es)
CA (1) CA2071196C (es)
DE (1) DE69032543T2 (es)
DK (1) DK0506825T3 (es)
ES (1) ES2121777T3 (es)
SG (1) SG46627A1 (es)
WO (1) WO1991009950A1 (es)

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Also Published As

Publication number Publication date
AU7176491A (en) 1991-07-24
AU646430B2 (en) 1994-02-24
JPH09224682A (ja) 1997-09-02
JP2774192B2 (ja) 1998-07-09
DE69032543T2 (de) 1999-04-15
SG46627A1 (en) 1998-02-20
JPH05504887A (ja) 1993-07-29
JP2790448B2 (ja) 1998-08-27
DK0506825T3 (da) 1999-05-03
EP0506825A1 (en) 1992-10-07
CA2071196C (en) 2002-04-23
AU681387B2 (en) 1997-08-28
DE69032543D1 (de) 1998-09-10
CA2071196A1 (en) 1991-06-23
ATE169337T1 (de) 1998-08-15
WO1991009950A1 (en) 1991-07-11
EP0506825B1 (en) 1998-08-05
AU6329694A (en) 1994-09-01

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