DE69032543D1 - Rekombinante expressionsvektoren und reinigungsverfahren für dns-polymerase aus thermus thermophilus - Google Patents

Rekombinante expressionsvektoren und reinigungsverfahren für dns-polymerase aus thermus thermophilus

Info

Publication number
DE69032543D1
DE69032543D1 DE69032543T DE69032543T DE69032543D1 DE 69032543 D1 DE69032543 D1 DE 69032543D1 DE 69032543 T DE69032543 T DE 69032543T DE 69032543 T DE69032543 T DE 69032543T DE 69032543 D1 DE69032543 D1 DE 69032543D1
Authority
DE
Germany
Prior art keywords
dna polymerase
thermus thermophilus
expression vectors
recombinant expression
cleaning method
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69032543T
Other languages
English (en)
Other versions
DE69032543T2 (de
Inventor
David Gelfand
Frances Lawyer
Susanne Stoffel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Application granted granted Critical
Publication of DE69032543D1 publication Critical patent/DE69032543D1/de
Publication of DE69032543T2 publication Critical patent/DE69032543T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
DE69032543T 1989-12-22 1990-12-21 Rekombinante expressionsvektoren und reinigungsverfahren für dns-polymerase aus thermus thermophilus Expired - Lifetime DE69032543T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US45596789A 1989-12-22 1989-12-22
PCT/US1990/007639 WO1991009950A1 (en) 1989-12-22 1990-12-21 Recombinant expression vectors and purification methods for thermus thermophilus dna polymerase

Publications (2)

Publication Number Publication Date
DE69032543D1 true DE69032543D1 (de) 1998-09-10
DE69032543T2 DE69032543T2 (de) 1999-04-15

Family

ID=23810915

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69032543T Expired - Lifetime DE69032543T2 (de) 1989-12-22 1990-12-21 Rekombinante expressionsvektoren und reinigungsverfahren für dns-polymerase aus thermus thermophilus

Country Status (10)

Country Link
EP (1) EP0506825B1 (de)
JP (2) JP2774192B2 (de)
AT (1) ATE169337T1 (de)
AU (2) AU646430B2 (de)
CA (1) CA2071196C (de)
DE (1) DE69032543T2 (de)
DK (1) DK0506825T3 (de)
ES (1) ES2121777T3 (de)
SG (1) SG46627A1 (de)
WO (1) WO1991009950A1 (de)

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US6077664A (en) * 1995-06-07 2000-06-20 Promega Corporation Thermophilic DNA polymerases from Thermotoga neapolitana
US6001645A (en) * 1995-06-07 1999-12-14 Promega Corporation Thermophilic DNA polymerases from thermotoga neapolitana
US5773258A (en) * 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
US6875572B2 (en) 1996-01-24 2005-04-05 Third Wave Technologies, Inc. Nucleic acid detection assays
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Also Published As

Publication number Publication date
AU7176491A (en) 1991-07-24
AU646430B2 (en) 1994-02-24
JPH09224682A (ja) 1997-09-02
JP2774192B2 (ja) 1998-07-09
DE69032543T2 (de) 1999-04-15
SG46627A1 (en) 1998-02-20
ES2121777T3 (es) 1998-12-16
JPH05504887A (ja) 1993-07-29
JP2790448B2 (ja) 1998-08-27
DK0506825T3 (da) 1999-05-03
EP0506825A1 (de) 1992-10-07
CA2071196C (en) 2002-04-23
AU681387B2 (en) 1997-08-28
CA2071196A1 (en) 1991-06-23
ATE169337T1 (de) 1998-08-15
WO1991009950A1 (en) 1991-07-11
EP0506825B1 (de) 1998-08-05
AU6329694A (en) 1994-09-01

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