ES2056123T3 - Clonacion del gen que codifica la enzima isoamilasa y su empleo en la produccion de dicha enzima. - Google Patents

Clonacion del gen que codifica la enzima isoamilasa y su empleo en la produccion de dicha enzima.

Info

Publication number
ES2056123T3
ES2056123T3 ES88830322T ES88830322T ES2056123T3 ES 2056123 T3 ES2056123 T3 ES 2056123T3 ES 88830322 T ES88830322 T ES 88830322T ES 88830322 T ES88830322 T ES 88830322T ES 2056123 T3 ES2056123 T3 ES 2056123T3
Authority
ES
Spain
Prior art keywords
isoamylase
enzyme
novel
biological activity
gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES88830322T
Other languages
English (en)
Inventor
Angelo Tognoni
Barbara Camerini
Giuliano Galli
Giuseppe Lucchese
Guido Grandi
Gennaro Carlo Di
Paolo Carrera
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Enichem Sintesi SpA
Original Assignee
Enichem Sintesi SpA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26327856&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2056123(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from IT8721615A external-priority patent/IT1228924B/it
Priority claimed from IT8821282A external-priority patent/IT1226901B/it
Application filed by Enichem Sintesi SpA filed Critical Enichem Sintesi SpA
Application granted granted Critical
Publication of ES2056123T3 publication Critical patent/ES2056123T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C12N9/246—Isoamylase (3.2.1.68)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01068—Isoamylase (3.2.1.68)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

UN NUEVO FRAGMENTO DE ADN (FIGURA7) OBTENIDO A PARTIR DE BACTERIAS DEL GENERO PSEUDOMONAS Y QUE CONTIENE EL GEN PARA LA ENZIMA AMILASA. TAMBIEN CONSTITUYEN NOVEDAD LAS MOLECULAS DE ADN RECONBINANTE QUE PERMITEN LA EXPRESION EN MICROORGANISMOS HOSPEDADORES QUE CONTENGAN EL YA MENCIONADO FRAGMENTO DE ADN O REGIONES CODIFICADORAS PARA LA ISOAMILASA MADURA O PARA UN POLIPEPTIDO CON ACTIVIDAD BIOLOGICA EQUIVALENTE. TAMBIEN SON NUEVOS LOS MICROORGANISMOS TRANSFORMADOS POR LAS MENCIONADAS MOLECULAS DE ADN RECONBINANTES Y QUE SON CAPACES DE EXPRESAR Y/O LA ENZIMA ISOAMILASA MADURA O EL POLIPEPTIDO CON ACTIVIDAD BIOLOGICA EQUIVALENTE. LA ISOAMILASA RESULTANTE ES ESPECIFICA EN TECNICAS DE SECUENCIAS DE AMINOACIDOS ESTRUCTURA Y ACTIVIDAD BIOLOGICA.
ES88830322T 1987-08-07 1988-07-26 Clonacion del gen que codifica la enzima isoamilasa y su empleo en la produccion de dicha enzima. Expired - Lifetime ES2056123T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IT8721615A IT1228924B (it) 1987-08-07 1987-08-07 Clonaggio del gene dell'isoamilasi.
IT8821282A IT1226901B (it) 1988-07-08 1988-07-08 Clonaggio del gene che codifica l'enzima isoamilasi e suo impiego per la produzione di detto enzima o suoi analoghi.

Publications (1)

Publication Number Publication Date
ES2056123T3 true ES2056123T3 (es) 1994-10-01

Family

ID=26327856

Family Applications (1)

Application Number Title Priority Date Filing Date
ES88830322T Expired - Lifetime ES2056123T3 (es) 1987-08-07 1988-07-26 Clonacion del gen que codifica la enzima isoamilasa y su empleo en la produccion de dicha enzima.

Country Status (7)

Country Link
US (1) US5457037A (es)
EP (1) EP0302838B1 (es)
JP (1) JP2777805B2 (es)
AT (1) ATE107959T1 (es)
CA (1) CA1308373C (es)
DE (1) DE3850456T2 (es)
ES (1) ES2056123T3 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2838798B2 (ja) * 1988-02-04 1998-12-16 株式会社林原生物化学研究所 イソアミラーゼ活性を有するポリペプチドとその用途
JP3633648B2 (ja) * 1993-07-20 2005-03-30 株式会社林原生物化学研究所 マルトース・トレハロース変換酵素とその製造方法並びに用途
EP0693558B1 (en) * 1994-07-19 2002-12-04 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo Trehalose and its production and use
US5750876A (en) * 1994-07-28 1998-05-12 Monsanto Company Isoamylase gene, compositions containing it, and methods of using isoamylases
CN102482658B (zh) * 2009-08-21 2015-07-15 诺维信公司 具有异淀粉酶活性的多肽和编码该多肽的多核苷酸
CN112980755B (zh) * 2019-12-13 2023-05-12 中国科学院天津工业生物技术研究所 高效分泌异淀粉酶的基因工程菌

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3560345A (en) * 1967-06-02 1971-02-02 Hayashibara Co Process for producing bacterial isoamylase
JPS543938B1 (es) * 1968-11-26 1979-02-28
US4335208A (en) * 1980-03-11 1982-06-15 Novo Industri A/S Saccharification of starch hydrolysates
US4879230A (en) * 1985-09-27 1989-11-07 Nikka Whisky Distilling Co., Ltd. Escherichia coli Candida maltosa Saccharomyces cerevisiae shuttle vectors and method for making

Also Published As

Publication number Publication date
EP0302838B1 (en) 1994-06-29
US5457037A (en) 1995-10-10
EP0302838A2 (en) 1989-02-08
JPH02457A (ja) 1990-01-05
JP2777805B2 (ja) 1998-07-23
CA1308373C (en) 1992-10-06
DE3850456D1 (de) 1994-08-04
DE3850456T2 (de) 1994-12-15
EP0302838A3 (en) 1989-11-08
ATE107959T1 (de) 1994-07-15

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