SE451461B - Plasmid med nukleotidsekvens innehallande en sekvens som kodar for a- eller b-kedjan i insulin och forfarande for dess framstellning samt e.coli innehallande plasmiden och forfarande for framstellning av e.coli - Google Patents
Plasmid med nukleotidsekvens innehallande en sekvens som kodar for a- eller b-kedjan i insulin och forfarande for dess framstellning samt e.coli innehallande plasmiden och forfarande for framstellning av e.coliInfo
- Publication number
- SE451461B SE451461B SE7806086A SE7806086A SE451461B SE 451461 B SE451461 B SE 451461B SE 7806086 A SE7806086 A SE 7806086A SE 7806086 A SE7806086 A SE 7806086A SE 451461 B SE451461 B SE 451461B
- Authority
- SE
- Sweden
- Prior art keywords
- plasmid
- procedure
- dna
- coli
- insulin
- Prior art date
Links
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 title abstract 4
- 239000002773 nucleotide Substances 0.000 title abstract 3
- 102000004877 Insulin Human genes 0.000 title abstract 2
- 108090001061 Insulin Proteins 0.000 title abstract 2
- 229940125396 insulin Drugs 0.000 title abstract 2
- 239000013612 plasmid Substances 0.000 title 2
- 108020004635 Complementary DNA Proteins 0.000 abstract 3
- 238000010804 cDNA synthesis Methods 0.000 abstract 3
- 239000002299 complementary DNA Substances 0.000 abstract 3
- 244000005700 microbiome Species 0.000 abstract 3
- 125000003729 nucleotide group Chemical group 0.000 abstract 2
- 108020004414 DNA Proteins 0.000 abstract 1
- 102000018997 Growth Hormone Human genes 0.000 abstract 1
- 108010051696 Growth Hormone Proteins 0.000 abstract 1
- 101000976075 Homo sapiens Insulin Proteins 0.000 abstract 1
- 102000002265 Human Growth Hormone Human genes 0.000 abstract 1
- 108010000521 Human Growth Hormone Proteins 0.000 abstract 1
- 239000000854 Human Growth Hormone Substances 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 230000004151 fermentation Effects 0.000 abstract 1
- 238000000855 fermentation Methods 0.000 abstract 1
- 239000000122 growth hormone Substances 0.000 abstract 1
- PBGKTOXHQIOBKM-FHFVDXKLSA-N insulin (human) Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@H]1CSSC[C@H]2C(=O)N[C@H](C(=O)N[C@@H](CO)C(=O)N[C@H](C(=O)N[C@H](C(N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3C=CC(O)=CC=3)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3NC=NC=3)NC(=O)[C@H](CO)NC(=O)CNC1=O)C(=O)NCC(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)NCC(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)O)C(O)=O)C(=O)N[C@@H](CC(N)=O)C(O)=O)=O)CSSC[C@@H](C(N2)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@@H](NC(=O)CN)[C@@H](C)CC)[C@@H](C)CC)[C@@H](C)O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H](NC(=O)[C@@H](N)CC=1C=CC=CC=1)C(C)C)C1=CN=CN1 PBGKTOXHQIOBKM-FHFVDXKLSA-N 0.000 abstract 1
- 108020004999 messenger RNA Proteins 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/57518—Placental lactogen; Chorionic somatomammotropin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Endocrinology (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Bioinformatics & Computational Biology (AREA)
- Pregnancy & Childbirth (AREA)
- Diabetes (AREA)
- Crystallography & Structural Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US80134377A | 1977-05-27 | 1977-05-27 | |
| US80502377A | 1977-06-09 | 1977-06-09 | |
| US89770978A | 1978-04-19 | 1978-04-19 | |
| US05897710 US4363877B1 (en) | 1977-09-23 | 1978-04-19 | Recombinant dna transfer vectors |
| US05/898,887 US4264731A (en) | 1977-05-27 | 1978-04-21 | DNA Joining method |
| US89900378A | 1978-04-26 | 1978-04-26 | |
| US89900278A | 1978-04-26 | 1978-04-26 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| SE7806086L SE7806086L (sv) | 1978-11-28 |
| SE451461B true SE451461B (sv) | 1987-10-12 |
Family
ID=27569921
Family Applications (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SE7806086A SE451461B (sv) | 1977-05-27 | 1978-05-26 | Plasmid med nukleotidsekvens innehallande en sekvens som kodar for a- eller b-kedjan i insulin och forfarande for dess framstellning samt e.coli innehallande plasmiden och forfarande for framstellning av e.coli |
| SE8305328A SE8305328L (sv) | 1977-05-27 | 1983-09-29 | Isolering av icke nedbruten rna |
| SE8305329A SE455794B (sv) | 1977-05-27 | 1983-09-29 | Plasmid med nukleotidsekvens som kodar for aminosyrasekvensen i humant tillvexthormon och forfarande for framstellning derav |
| SE8305327A SE8305327D0 (sv) | 1977-05-27 | 1983-09-29 | Isolering av differentierade celler |
Family Applications After (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SE8305328A SE8305328L (sv) | 1977-05-27 | 1983-09-29 | Isolering av icke nedbruten rna |
| SE8305329A SE455794B (sv) | 1977-05-27 | 1983-09-29 | Plasmid med nukleotidsekvens som kodar for aminosyrasekvensen i humant tillvexthormon och forfarande for framstellning derav |
| SE8305327A SE8305327D0 (sv) | 1977-05-27 | 1983-09-29 | Isolering av differentierade celler |
Country Status (21)
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1989010937A1 (en) * | 1988-05-11 | 1989-11-16 | Novo Nordisk A/S | Insulin analogues |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ZA782933B (en) * | 1977-09-23 | 1979-05-30 | Univ California | Purification of nucleotide sequences suitable for expression in bacteria |
| FR2422717B1 (fr) * | 1977-11-08 | 1985-12-06 | Genentech Inc | Procede permettant l'expression microbienne de polypeptides et moyens appropries |
| FI792481A7 (fi) * | 1978-08-11 | 1981-01-01 | The Regents Of The Univ Of | Enkaroyyttisen proteiinin synteesi mikro-organismia käyttäen. |
| US6297355B1 (en) | 1978-12-22 | 2001-10-02 | Biogen, Inc. | Polypeptides displaying HBV antigenicity or hbv antigen specificity |
| JPS55150883A (en) * | 1979-05-11 | 1980-11-25 | Nakano Vinegar Co Ltd | Method of storing "mozuku" |
| GR68404B (enrdf_load_stackoverflow) * | 1979-06-01 | 1981-12-29 | Univ California | |
| FR2458584A1 (fr) * | 1979-06-08 | 1981-01-02 | Pasteur Institut | Vecteurs pour le transfert et l'expression de materiel genetique dans une cellule eucaryote et procede pour la production d'une proteine determinee dans des cellules eucaryotes |
| US4342832A (en) * | 1979-07-05 | 1982-08-03 | Genentech, Inc. | Method of constructing a replicable cloning vehicle having quasi-synthetic genes |
| GR70279B (enrdf_load_stackoverflow) * | 1979-09-12 | 1982-09-03 | Univ California | |
| IL58765A (en) * | 1979-11-21 | 1986-09-30 | Yeda Res & Dev | Process for the production of essentially pure messenger rna of human fibroblast interferon and process for the production of interferon beta |
| DE3001928A1 (de) * | 1980-01-19 | 1981-08-06 | Hoechst Ag, 6000 Frankfurt | Genprodukt eines hoeheren organismus aus einem dieses gen enthaltenden mikroorganismus |
| IL61982A (en) * | 1980-02-15 | 1984-01-31 | Cpc International Inc | Genetically engineered microorganisms for massive production of amyloytic enzymes and process for preparing same using the corresponding recombinant dnas containing amylase coding genes |
| US4350764A (en) * | 1980-03-10 | 1982-09-21 | The Regents Of The University Of California | Microbiological synthesis of beta endorphin |
| CA1202581A (en) * | 1980-03-27 | 1986-04-01 | Saran A. Narang | Adaptor molecules for dna and their application to synthesis of gene-derived products |
| US4370417A (en) * | 1980-04-03 | 1983-01-25 | Abbott Laboratories | Recombinant deoxyribonucleic acid which codes for plasminogen activator |
| US4338397A (en) | 1980-04-11 | 1982-07-06 | President And Fellows Of Harvard College | Mature protein synthesis |
| ATE22327T1 (de) * | 1980-12-12 | 1986-10-15 | Unilever Nv | Dna-sequenzen fuer verschiedene allele formen des reifen thaumatins, diese sequenzen enthaltende rekombinante plasmide, verfahren zur herstellung dieser plasmide, diese rekombinante plasmide enthaltende bakterielle kulturen und verfahren zur herstellung von reifen thaumatin. |
| DE3177281T2 (de) * | 1980-12-12 | 1992-12-10 | Unilever Nv | Strukturgene, die fuer die verschiedenen allelischen und reifungsformen von praeprothaumatin und deren mutanten kodieren, diese strukturgene enthaltende klonierungsvektoren und deren expression in mikrobiellen wirtzellen. |
| US4695543A (en) * | 1982-03-23 | 1987-09-22 | Bristol-Myers Company | Alpha Interferon GX-1 |
| US4748233A (en) * | 1982-03-23 | 1988-05-31 | Bristol-Myers Company | Alpha-interferon Gx-1 |
| CA1224432A (en) * | 1982-08-17 | 1987-07-21 | Gary N. Buell | Dna sequences, recombinant dna molecules and processes for producing bovine growth hormone-like polypeptides in high yield |
| US5474980A (en) * | 1984-10-04 | 1995-12-12 | Monsanto Company | Prolonged release of biologically active somatotropins |
| US5411951A (en) * | 1984-10-04 | 1995-05-02 | Monsanto Company | Prolonged release of biologically active somatotropin |
| DE4034702A1 (de) * | 1990-10-31 | 1992-05-07 | Siemens Ag | Verfahren und vorrichtung zur pruefung der aktiven werkzeuge von gesenkbiegemaschinen, insbesondere von biegestempeln |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1521032A (en) * | 1974-08-08 | 1978-08-09 | Ici Ltd | Biological treatment |
-
1978
- 1978-05-17 NZ NZ187300A patent/NZ187300A/xx unknown
- 1978-05-23 NL NL7805591A patent/NL7805591A/ not_active Application Discontinuation
- 1978-05-23 AR AR272286A patent/AR225404A1/es active
- 1978-05-23 GR GR56309A patent/GR73552B/el unknown
- 1978-05-24 DE DE19782822568 patent/DE2822568A1/de active Granted
- 1978-05-24 BE BE187995A patent/BE867424A/xx not_active IP Right Cessation
- 1978-05-24 PT PT68082A patent/PT68082B/pt unknown
- 1978-05-24 DE DE2858357A patent/DE2858357C2/de not_active Expired - Lifetime
- 1978-05-25 AU AU36488/78A patent/AU521903B2/en not_active Expired
- 1978-05-25 DD DD78205588A patent/DD145927A5/de unknown
- 1978-05-25 FR FR7815596A patent/FR2392033B1/fr not_active Expired
- 1978-05-25 GB GB22417/78A patent/GB1565190A/en not_active Expired
- 1978-05-26 LU LU79714A patent/LU79714A1/xx unknown
- 1978-05-26 IL IL54790A patent/IL54790A/xx unknown
- 1978-05-26 CH CH581078A patent/CH642680A5/de not_active IP Right Cessation
- 1978-05-26 FI FI781675A patent/FI64640C/fi not_active IP Right Cessation
- 1978-05-26 SE SE7806086A patent/SE451461B/xx unknown
- 1978-05-26 DK DK782332A patent/DK233278A/da not_active Application Discontinuation
- 1978-05-27 JP JP53063756A patent/JPH0659218B2/ja not_active Expired - Lifetime
- 1978-05-27 BG BG039878A patent/BG40319A3/xx unknown
- 1978-05-27 PL PL1978207176A patent/PL140200B1/pl unknown
- 1978-05-29 IT IT23930/78A patent/IT1094934B/it active
-
1983
- 1983-09-29 SE SE8305328A patent/SE8305328L/sv not_active Application Discontinuation
- 1983-09-29 SE SE8305329A patent/SE455794B/sv not_active Application Discontinuation
- 1983-09-29 SE SE8305327A patent/SE8305327D0/xx not_active Application Discontinuation
-
1991
- 1991-01-04 DK DK000791A patent/DK791D0/da not_active Application Discontinuation
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1989010937A1 (en) * | 1988-05-11 | 1989-11-16 | Novo Nordisk A/S | Insulin analogues |
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