SA519401607B1 - اختبارات خلوية وفي المعمل لقياس نشاط السموم العصبية للبوتولينوم - Google Patents

اختبارات خلوية وفي المعمل لقياس نشاط السموم العصبية للبوتولينوم

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Publication number
SA519401607B1
SA519401607B1 SA519401607A SA519401607A SA519401607B1 SA 519401607 B1 SA519401607 B1 SA 519401607B1 SA 519401607 A SA519401607 A SA 519401607A SA 519401607 A SA519401607 A SA 519401607A SA 519401607 B1 SA519401607 B1 SA 519401607B1
Authority
SA
Saudi Arabia
Prior art keywords
activity
neurotoxin
polypeptides
disclosed
vitro
Prior art date
Application number
SA519401607A
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English (en)
Inventor
مين دونج
فيفان يو
Original Assignee
بريسدينت اند فيلوس اوف هارفارد كوليج
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by بريسدينت اند فيلوس اوف هارفارد كوليج filed Critical بريسدينت اند فيلوس اوف هارفارد كوليج
Publication of SA519401607B1 publication Critical patent/SA519401607B1/ar

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24—Metalloendopeptidases (3.4.24)
    • C12Y304/24069—Bontoxilysin (3.4.24.69), i.e. botulinum neurotoxin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/33—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Clostridium (G)

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

يتعلق الاختراع الحالي بوسيلة للكشف عن وتحديد خصائص السموم العصبية neurotoxins مثل السم العصبي للبوتولينوم botulinum neurotoxin (BoNT) أو السم العصبي للتيتانوس tetanus neurotoxin. ويقدم الكشف الحالي طرقًا لتحديد فعالية ونشاط السموم العصبية في المعمل وفي جسم الكائن الحي. يتم الكشف أيضًا عن بولي ببتيدات polypeptides تحتوي على شظيات عند الطرف N والطرف C لبروتين مراسل reporter protein والتي يتم تقسيمها بواسطة رابط linker يشتمل على موقع شطر للسم العصبي neurotoxin cleavage site. إن انشطار الرابط بواسطة السم العصبي يقلل من نشاط البروتين المراسل، مما يدل على نشاط السم العصبي. يتم أيضًا الكشف عن تركيبات وأطقم تحتوي على البولي ببتيدات التي يتم الكشف عنها، وأحماض نووية nucleic acids تحتوي على سلاسل نوكليوتيدات nucleotide sequences التي تشفر هذه البولي ببتيدات، وخلايا تعبر عن هذه البولي ببتيدات. شكل1
SA519401607A 2016-10-20 2019-04-18 اختبارات خلوية وفي المعمل لقياس نشاط السموم العصبية للبوتولينوم SA519401607B1 (ar)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662410558P 2016-10-20 2016-10-20
PCT/US2017/057411 WO2018075783A2 (en) 2016-10-20 2017-10-19 In vitro and cell based assays for measuring the activity of botulinum neurotoxins

Publications (1)

Publication Number Publication Date
SA519401607B1 true SA519401607B1 (ar) 2022-08-04

Family

ID=60413254

Family Applications (1)

Application Number Title Priority Date Filing Date
SA519401607A SA519401607B1 (ar) 2016-10-20 2019-04-18 اختبارات خلوية وفي المعمل لقياس نشاط السموم العصبية للبوتولينوم

Country Status (19)

Country Link
US (2) US11306347B2 (ar)
EP (1) EP3529616B1 (ar)
JP (2) JP7227901B2 (ar)
KR (1) KR102556363B1 (ar)
CN (1) CN110088624B (ar)
AU (1) AU2017345560A1 (ar)
BR (1) BR112019007831A2 (ar)
CA (1) CA3040507A1 (ar)
DK (1) DK3529616T3 (ar)
EA (1) EA039761B1 (ar)
ES (1) ES2963830T3 (ar)
FI (1) FI3529616T3 (ar)
HU (1) HUE064332T2 (ar)
MX (2) MX2019004431A (ar)
PL (1) PL3529616T3 (ar)
PT (1) PT3529616T (ar)
SA (1) SA519401607B1 (ar)
SG (1) SG11201903523YA (ar)
WO (1) WO2018075783A2 (ar)

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US9394537B2 (en) 2010-12-22 2016-07-19 President And Fellows Of Harvard College Continuous directed evolution
US10920208B2 (en) 2014-10-22 2021-02-16 President And Fellows Of Harvard College Evolution of proteases
US11299729B2 (en) 2015-04-17 2022-04-12 President And Fellows Of Harvard College Vector-based mutagenesis system
US10392674B2 (en) 2015-07-22 2019-08-27 President And Fellows Of Harvard College Evolution of site-specific recombinases
US11524983B2 (en) 2015-07-23 2022-12-13 President And Fellows Of Harvard College Evolution of Bt toxins
WO2017019895A1 (en) 2015-07-30 2017-02-02 President And Fellows Of Harvard College Evolution of talens
CA3024331A1 (en) * 2016-05-16 2017-11-23 President And Fellows Of Harvard College Method for purification and activation of botulinum neurotoxin
KR102556363B1 (ko) 2016-10-20 2023-07-18 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 보툴리눔 신경독소의 활성을 측정하기 위한 시험관내 및 세포 기반 검정
WO2019010164A1 (en) 2017-07-06 2019-01-10 President And Fellows Of Harvard College EVOLUTION OF ARNT SYNTHÉTASES
US11624130B2 (en) 2017-09-18 2023-04-11 President And Fellows Of Harvard College Continuous evolution for stabilized proteins
WO2019241649A1 (en) 2018-06-14 2019-12-19 President And Fellows Of Harvard College Evolution of cytidine deaminases
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Also Published As

Publication number Publication date
FI3529616T3 (fi) 2023-12-04
US20190276873A1 (en) 2019-09-12
EA039761B1 (ru) 2022-03-10
CN110088624A (zh) 2019-08-02
US11306347B2 (en) 2022-04-19
AU2017345560A1 (en) 2019-05-02
WO2018075783A2 (en) 2018-04-26
EP3529616B1 (en) 2023-09-06
ES2963830T3 (es) 2024-04-02
KR20190068582A (ko) 2019-06-18
KR102556363B1 (ko) 2023-07-18
PL3529616T3 (pl) 2024-03-18
HUE064332T2 (hu) 2024-03-28
US11788069B2 (en) 2023-10-17
JP2019532651A (ja) 2019-11-14
SG11201903523YA (en) 2019-05-30
MX2023004733A (es) 2023-05-10
WO2018075783A3 (en) 2018-06-21
BR112019007831A2 (pt) 2019-10-01
JP7227901B2 (ja) 2023-02-22
CA3040507A1 (en) 2018-04-26
EA201990929A1 (ru) 2019-10-31
DK3529616T3 (da) 2023-12-11
MX2019004431A (es) 2019-08-14
CN110088624B (zh) 2024-01-16
JP2023011700A (ja) 2023-01-24
PT3529616T (pt) 2023-12-06
EP3529616A2 (en) 2019-08-28
US20220356508A1 (en) 2022-11-10

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