MX2019004431A - Pruebas in vitro y con base en celula para medir la actividad de neurotoxinas botulinicas. - Google Patents

Pruebas in vitro y con base en celula para medir la actividad de neurotoxinas botulinicas.

Info

Publication number
MX2019004431A
MX2019004431A MX2019004431A MX2019004431A MX2019004431A MX 2019004431 A MX2019004431 A MX 2019004431A MX 2019004431 A MX2019004431 A MX 2019004431A MX 2019004431 A MX2019004431 A MX 2019004431A MX 2019004431 A MX2019004431 A MX 2019004431A
Authority
MX
Mexico
Prior art keywords
activity
neurotoxin
polypeptides
disclosed
vitro
Prior art date
Application number
MX2019004431A
Other languages
English (en)
Inventor
Dong Min
Yu Feifan
Original Assignee
Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard College filed Critical Harvard College
Publication of MX2019004431A publication Critical patent/MX2019004431A/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24—Metalloendopeptidases (3.4.24)
    • C12Y304/24069—Bontoxilysin (3.4.24.69), i.e. botulinum neurotoxin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/33—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Clostridium (G)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

En la presente se describen medios para la detección y caracterización de neurotoxinas como la neurotoxina botulínica (BoNT) o neurotoxina tetánica La presente descripción proporciona métodos para determinar la potencia y actividad de las neurotoxinas in vitro e in vivo. También se describen polipéptidos que comprenden fragmentos de extremo N y C de una proteína indicadora que se dividen mediante un enlazador que comprende un sitio de escisión de neurotoxina. La escisión del enlazador por una neurotoxina disminuye la actividad de la proteína indicadora, lo que indica la actividad de la neurotoxina. También se describen las composiciones y kits que comprenden los polipéptidos descritos, los ácidos nucleicos que comprenden secuencias de nucleótidos que codifican dichos polipéptidos y las células que expresan dichos polipéptidos.
MX2019004431A 2016-10-20 2017-10-19 Pruebas in vitro y con base en celula para medir la actividad de neurotoxinas botulinicas. MX2019004431A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662410558P 2016-10-20 2016-10-20
PCT/US2017/057411 WO2018075783A2 (en) 2016-10-20 2017-10-19 In vitro and cell based assays for measuring the activity of botulinum neurotoxins

Publications (1)

Publication Number Publication Date
MX2019004431A true MX2019004431A (es) 2019-08-14

Family

ID=60413254

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2019004431A MX2019004431A (es) 2016-10-20 2017-10-19 Pruebas in vitro y con base en celula para medir la actividad de neurotoxinas botulinicas.
MX2023004733A MX2023004733A (es) 2016-10-20 2019-04-15 Pruebas in vitro y con base en celula para medir la actividad de neurotoxinas botulinicas.

Family Applications After (1)

Application Number Title Priority Date Filing Date
MX2023004733A MX2023004733A (es) 2016-10-20 2019-04-15 Pruebas in vitro y con base en celula para medir la actividad de neurotoxinas botulinicas.

Country Status (19)

Country Link
US (2) US11306347B2 (es)
EP (1) EP3529616B1 (es)
JP (2) JP7227901B2 (es)
KR (1) KR102556363B1 (es)
CN (1) CN110088624B (es)
AU (1) AU2017345560A1 (es)
BR (1) BR112019007831A2 (es)
CA (1) CA3040507A1 (es)
DK (1) DK3529616T3 (es)
EA (1) EA039761B1 (es)
ES (1) ES2963830T3 (es)
FI (1) FI3529616T3 (es)
HU (1) HUE064332T2 (es)
MX (2) MX2019004431A (es)
PL (1) PL3529616T3 (es)
PT (1) PT3529616T (es)
SA (1) SA519401607B1 (es)
SG (1) SG11201903523YA (es)
WO (1) WO2018075783A2 (es)

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EP2655614B1 (en) 2010-12-22 2017-03-15 President and Fellows of Harvard College Continuous directed evolution
US10920208B2 (en) 2014-10-22 2021-02-16 President And Fellows Of Harvard College Evolution of proteases
US11299729B2 (en) 2015-04-17 2022-04-12 President And Fellows Of Harvard College Vector-based mutagenesis system
US10392674B2 (en) 2015-07-22 2019-08-27 President And Fellows Of Harvard College Evolution of site-specific recombinases
WO2017015559A2 (en) 2015-07-23 2017-01-26 President And Fellows Of Harvard College Evolution of bt toxins
US10612011B2 (en) 2015-07-30 2020-04-07 President And Fellows Of Harvard College Evolution of TALENs
EP3458472B1 (en) * 2016-05-16 2021-10-20 President and Fellows of Harvard College Method for purification and activation of botulinum neurotoxin
HUE064332T2 (hu) 2016-10-20 2024-03-28 Harvard College In vitro és sejtalapú esszék botulinum neurotoxinok aktivitásának mérésére
US11447809B2 (en) 2017-07-06 2022-09-20 President And Fellows Of Harvard College Evolution of tRNA synthetases
WO2019056002A1 (en) 2017-09-18 2019-03-21 President And Fellows Of Harvard College CONTINUOUS EVOLUTION FOR STABILIZED PROTEINS
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GB202213479D0 (en) 2022-09-14 2022-10-26 Ipsen Biopharm Ltd Cell-free clostridial neurotoxin assays
GB202404021D0 (en) 2024-03-20 2024-05-01 Ipsen Biopharm Ltd Cell-based neurotoxin assay

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Also Published As

Publication number Publication date
BR112019007831A2 (pt) 2019-10-01
WO2018075783A2 (en) 2018-04-26
US11788069B2 (en) 2023-10-17
EA039761B1 (ru) 2022-03-10
MX2023004733A (es) 2023-05-10
US11306347B2 (en) 2022-04-19
SA519401607B1 (ar) 2022-08-04
HUE064332T2 (hu) 2024-03-28
DK3529616T3 (da) 2023-12-11
SG11201903523YA (en) 2019-05-30
CA3040507A1 (en) 2018-04-26
KR20190068582A (ko) 2019-06-18
JP7227901B2 (ja) 2023-02-22
PT3529616T (pt) 2023-12-06
EP3529616A2 (en) 2019-08-28
JP2019532651A (ja) 2019-11-14
FI3529616T3 (fi) 2023-12-04
AU2017345560A1 (en) 2019-05-02
US20220356508A1 (en) 2022-11-10
PL3529616T3 (pl) 2024-03-18
EP3529616B1 (en) 2023-09-06
KR102556363B1 (ko) 2023-07-18
ES2963830T3 (es) 2024-04-02
US20190276873A1 (en) 2019-09-12
WO2018075783A3 (en) 2018-06-21
JP2023011700A (ja) 2023-01-24
EA201990929A1 (ru) 2019-10-31
CN110088624B (zh) 2024-01-16
CN110088624A (zh) 2019-08-02

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