DK3529616T3 - In vitro- og cellebaserede assays til måling af aktiviteten af botulinum-neurotoksiner - Google Patents

In vitro- og cellebaserede assays til måling af aktiviteten af botulinum-neurotoksiner Download PDF

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Publication number
DK3529616T3
DK3529616T3 DK17801528.5T DK17801528T DK3529616T3 DK 3529616 T3 DK3529616 T3 DK 3529616T3 DK 17801528 T DK17801528 T DK 17801528T DK 3529616 T3 DK3529616 T3 DK 3529616T3
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Denmark
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vitro
activity
measuring
cell
based assays
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DK17801528.5T
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English (en)
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Min Dong
Feifan Yu
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Harvard College
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0069Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y304/00Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24Metalloendopeptidases (3.4.24)
    • C12Y304/24069Bontoxilysin (3.4.24.69), i.e. botulinum neurotoxin
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/33Assays involving biological materials from specific organisms or of a specific nature from bacteria from Clostridium (G)

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK17801528.5T 2016-10-20 2017-10-19 In vitro- og cellebaserede assays til måling af aktiviteten af botulinum-neurotoksiner DK3529616T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662410558P 2016-10-20 2016-10-20
PCT/US2017/057411 WO2018075783A2 (en) 2016-10-20 2017-10-19 In vitro and cell based assays for measuring the activity of botulinum neurotoxins

Publications (1)

Publication Number Publication Date
DK3529616T3 true DK3529616T3 (da) 2023-12-11

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DK17801528.5T DK3529616T3 (da) 2016-10-20 2017-10-19 In vitro- og cellebaserede assays til måling af aktiviteten af botulinum-neurotoksiner

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Country Link
US (2) US11306347B2 (da)
EP (1) EP3529616B1 (da)
JP (2) JP7227901B2 (da)
KR (1) KR102556363B1 (da)
CN (1) CN110088624B (da)
AU (1) AU2017345560A1 (da)
BR (1) BR112019007831A2 (da)
CA (1) CA3040507A1 (da)
DK (1) DK3529616T3 (da)
EA (1) EA039761B1 (da)
ES (1) ES2963830T3 (da)
FI (1) FI3529616T3 (da)
HU (1) HUE064332T2 (da)
MX (2) MX2019004431A (da)
PL (1) PL3529616T3 (da)
PT (1) PT3529616T (da)
SA (1) SA519401607B1 (da)
SG (1) SG11201903523YA (da)
WO (1) WO2018075783A2 (da)

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EP2655614B1 (en) 2010-12-22 2017-03-15 President and Fellows of Harvard College Continuous directed evolution
US10920208B2 (en) 2014-10-22 2021-02-16 President And Fellows Of Harvard College Evolution of proteases
US11299729B2 (en) 2015-04-17 2022-04-12 President And Fellows Of Harvard College Vector-based mutagenesis system
US10392674B2 (en) 2015-07-22 2019-08-27 President And Fellows Of Harvard College Evolution of site-specific recombinases
WO2017015559A2 (en) 2015-07-23 2017-01-26 President And Fellows Of Harvard College Evolution of bt toxins
US10612011B2 (en) 2015-07-30 2020-04-07 President And Fellows Of Harvard College Evolution of TALENs
EP3458472B1 (en) * 2016-05-16 2021-10-20 President and Fellows of Harvard College Method for purification and activation of botulinum neurotoxin
HUE064332T2 (hu) 2016-10-20 2024-03-28 Harvard College In vitro és sejtalapú esszék botulinum neurotoxinok aktivitásának mérésére
US11447809B2 (en) 2017-07-06 2022-09-20 President And Fellows Of Harvard College Evolution of tRNA synthetases
WO2019056002A1 (en) 2017-09-18 2019-03-21 President And Fellows Of Harvard College CONTINUOUS EVOLUTION FOR STABILIZED PROTEINS
WO2019241649A1 (en) 2018-06-14 2019-12-19 President And Fellows Of Harvard College Evolution of cytidine deaminases
US20220259269A1 (en) * 2019-07-15 2022-08-18 President And Fellows Of Harvard College Evolved botulinum neurotoxins and uses thereof
WO2021195479A1 (en) * 2020-03-26 2021-09-30 Cellex, Inc. Protease assays and their applications
CN114540419A (zh) * 2022-03-04 2022-05-27 中国人民解放军军事科学院军事医学研究院 一种分析包膜病毒膜融合效率的三功能报告系统
GB202213479D0 (en) 2022-09-14 2022-10-26 Ipsen Biopharm Ltd Cell-free clostridial neurotoxin assays
GB202404021D0 (en) 2024-03-20 2024-05-01 Ipsen Biopharm Ltd Cell-based neurotoxin assay

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US5004565A (en) 1986-07-17 1991-04-02 The Board Of Governors Of Wayne State University Method and compositions providing enhanced chemiluminescence from 1,2-dioxetanes
US5374534A (en) 1990-07-19 1994-12-20 Charm Sciences, Inc. Method of preparing D-luciferin derivatives
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AU695623B2 (en) 1994-05-31 1998-08-20 Allergan, Inc. Modification of clostridial toxins for use as transport proteins
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Also Published As

Publication number Publication date
BR112019007831A2 (pt) 2019-10-01
WO2018075783A2 (en) 2018-04-26
US11788069B2 (en) 2023-10-17
EA039761B1 (ru) 2022-03-10
MX2023004733A (es) 2023-05-10
US11306347B2 (en) 2022-04-19
SA519401607B1 (ar) 2022-08-04
HUE064332T2 (hu) 2024-03-28
SG11201903523YA (en) 2019-05-30
CA3040507A1 (en) 2018-04-26
KR20190068582A (ko) 2019-06-18
JP7227901B2 (ja) 2023-02-22
PT3529616T (pt) 2023-12-06
MX2019004431A (es) 2019-08-14
EP3529616A2 (en) 2019-08-28
JP2019532651A (ja) 2019-11-14
FI3529616T3 (fi) 2023-12-04
AU2017345560A1 (en) 2019-05-02
US20220356508A1 (en) 2022-11-10
PL3529616T3 (pl) 2024-03-18
EP3529616B1 (en) 2023-09-06
KR102556363B1 (ko) 2023-07-18
ES2963830T3 (es) 2024-04-02
US20190276873A1 (en) 2019-09-12
WO2018075783A3 (en) 2018-06-21
JP2023011700A (ja) 2023-01-24
EA201990929A1 (ru) 2019-10-31
CN110088624B (zh) 2024-01-16
CN110088624A (zh) 2019-08-02

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