MX2018014066A - Metodo para purificacion y activacion de la neurotoxina botulinica. - Google Patents
Metodo para purificacion y activacion de la neurotoxina botulinica.Info
- Publication number
- MX2018014066A MX2018014066A MX2018014066A MX2018014066A MX2018014066A MX 2018014066 A MX2018014066 A MX 2018014066A MX 2018014066 A MX2018014066 A MX 2018014066A MX 2018014066 A MX2018014066 A MX 2018014066A MX 2018014066 A MX2018014066 A MX 2018014066A
- Authority
- MX
- Mexico
- Prior art keywords
- protein
- polypeptide
- purification
- conditions appropriate
- botulinum neurotoxin
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/24—Metalloendopeptidases (3.4.24)
- C12Y304/24069—Bontoxilysin (3.4.24.69), i.e. botulinum neurotoxin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Gastroenterology & Hepatology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
En la presente se describen métodos para el aislamiento y purificación de una proteína de neurotoxina botulínica (BoNT), o un polipéptido que comprende un dominio de unión al receptor de BoNT, de una solución. El método comprende poner en contacto la solución que contiene la proteína o polipéptido con una matriz que tiene unida a esta una no hemaglutinina no tóxica (NTNHA) en condiciones apropiadas para la unión, lavar la matriz para de este modo quitar los materiales no unidos, y eluir la proteína o polipéptido con una solución que disocia la proteína unida de la NTNHA. Las condiciones apropiadas para la unión son un pH de menos de 7.5 (por ejemplo, 6). Las condiciones apropiadas para la disociación son un pH mayor que o igual a 7.5 (por ejemplo, 8). Las composiciones específicas a los métodos también se describen.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662336958P | 2016-05-16 | 2016-05-16 | |
| PCT/US2017/032985 WO2017201105A1 (en) | 2016-05-16 | 2017-05-16 | Method for purification and activation of botulinum neurotoxin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2018014066A true MX2018014066A (es) | 2019-04-04 |
Family
ID=59014734
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2018014066A MX2018014066A (es) | 2016-05-16 | 2017-05-16 | Metodo para purificacion y activacion de la neurotoxina botulinica. |
Country Status (16)
| Country | Link |
|---|---|
| US (1) | US11242515B2 (es) |
| EP (1) | EP3458472B1 (es) |
| JP (1) | JP7060522B2 (es) |
| KR (1) | KR102567311B1 (es) |
| CN (1) | CN109641941B (es) |
| AU (1) | AU2017268285B2 (es) |
| BR (1) | BR112018073591A2 (es) |
| CA (1) | CA3024331A1 (es) |
| EA (1) | EA201892643A1 (es) |
| ES (1) | ES2904269T3 (es) |
| MX (1) | MX2018014066A (es) |
| SA (1) | SA518400443B1 (es) |
| SG (1) | SG11201809903WA (es) |
| UA (1) | UA127310C2 (es) |
| WO (1) | WO2017201105A1 (es) |
| ZA (1) | ZA201807626B (es) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102083371B1 (ko) | 2012-11-21 | 2020-03-04 | 입센 바이오이노베이션 리미티드 | 단백질 가수분해 처리된 폴리펩티드의 제조방법 |
| WO2017201105A1 (en) * | 2016-05-16 | 2017-11-23 | President And Fellows Of Harvard College | Method for purification and activation of botulinum neurotoxin |
| US11129906B1 (en) | 2016-12-07 | 2021-09-28 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
| SE542539C2 (en) * | 2018-02-26 | 2020-06-02 | Toxotech Ab | Chimeric botulinum neurotoxin heavy chain binding domain |
| WO2020129027A1 (en) * | 2018-12-21 | 2020-06-25 | King Abdullah University Of Science And Technology | Sliding clamp-based affinity purification systems, methods of making and use thereof |
| CN110208411B (zh) * | 2019-06-10 | 2021-12-24 | 浙江龙传生物医药科技有限公司 | 用于药物代谢检测的羧酸酯酶抑制剂制剂 |
| CN114958887A (zh) * | 2021-02-26 | 2022-08-30 | 重庆誉颜制药有限公司 | 一种经修饰的毒素多肽的制备方法 |
Family Cites Families (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4703004A (en) | 1984-01-24 | 1987-10-27 | Immunex Corporation | Synthesis of protein with an identification peptide |
| US5011912A (en) | 1986-12-19 | 1991-04-30 | Immunex Corporation | Hybridoma and monoclonal antibody for use in an immunoaffinity purification system |
| US4851341A (en) | 1986-12-19 | 1989-07-25 | Immunex Corporation | Immunoaffinity purification system |
| CA1340522C (en) | 1987-03-10 | 1999-05-04 | Heinz Dobeli | Fusion proteins containing neighbouring histidines for improved purification |
| US5654176A (en) | 1987-05-28 | 1997-08-05 | Amrad Corporation Limited | Fusion proteins containing glutathione-s-transferase |
| US5599903A (en) | 1992-04-03 | 1997-02-04 | Terrapin Technologies, Inc. | Glutathione analogs and paralog panels comprising glutathione mimics |
| GB9411138D0 (en) | 1994-06-03 | 1994-07-27 | Microbiological Res Authority | Toxin assay |
| AUPN015794A0 (en) | 1994-12-20 | 1995-01-19 | Csl Limited | Variants of human papilloma virus antigens |
| KR20030060150A (ko) * | 2002-01-07 | 2003-07-16 | (주)메디톡스 | 클로스트리디움 보툴리눔 ａ형 독소를 정제하는 방법 |
| JP2005538954A (ja) * | 2002-05-31 | 2005-12-22 | トーマス・ジェファーソン・ユニバーシティ | 分子の経上皮輸送用組成物及び方法 |
| US7148041B2 (en) | 2003-09-25 | 2006-12-12 | Allergan, Inc. | Animal product free media and processes for obtaining a botulinum toxin |
| JP5178009B2 (ja) * | 2003-12-19 | 2013-04-10 | ウィスコンシン アルムニ リサーチ ファンデイション | ボツリヌス神経毒素検出のための方法および複合体 |
| WO2006099590A2 (en) * | 2005-03-15 | 2006-09-21 | Allergan, Inc. | Modified clostridial toxins with altered targeting capabilities for clostridial toxin target cells |
| US7985554B2 (en) * | 2005-10-14 | 2011-07-26 | Wisconsin Alumni Research Foundation | Botulinum neurotoxin A receptor and the use thereof |
| JP2009132686A (ja) * | 2007-10-26 | 2009-06-18 | Okayama Univ | ボツリヌス毒素由来のポリペプチド及びボツリヌス毒素の検出方法 |
| JP2010037253A (ja) * | 2008-08-04 | 2010-02-18 | Chemo Sero Therapeut Res Inst | ボツリヌス毒素に対するモノクローナル抗体および該抗体を用いたボツリヌス神経毒素精製方法 |
| WO2010039748A2 (en) * | 2008-09-30 | 2010-04-08 | Wisconsin Alumni Research Foundation | Botulinum neurotoxin e receptors and uses thereof |
| MX2012006985A (es) * | 2009-12-16 | 2012-09-12 | Allergan Inc | Toxinas clostridium modificadas que comprenden un dominio de enlace al sitio de escision de la proteasa integrado. |
| ES2634261T3 (es) | 2011-05-19 | 2017-09-27 | Ipsen Bioinnovation Limited | Métodos para la fabricación de polipéptidos procesados proteolíticamente |
| DK3372239T3 (da) | 2012-05-30 | 2021-02-01 | Harvard College | Manipuleret botulinumneurotoksin |
| KR101339349B1 (ko) * | 2013-08-02 | 2013-12-09 | 주식회사 대웅 | 보툴리눔 독소의 제조방법 |
| WO2017201105A1 (en) | 2016-05-16 | 2017-11-23 | President And Fellows Of Harvard College | Method for purification and activation of botulinum neurotoxin |
| CN109476713A (zh) * | 2016-06-08 | 2019-03-15 | 儿童医学中心公司 | 工程改造的肉毒杆菌神经毒素 |
| DK3481852T3 (da) * | 2016-07-08 | 2023-02-27 | Childrens Medical Center | Hidtil ukendt botulinum-neurotoksin og dets derivater |
| KR102463881B1 (ko) * | 2016-10-04 | 2022-11-07 | (주)메디톡스 | 보툴리눔 독소 함유 용액으로부터 보툴리눔 독소를 분리하는 방법 |
| SG11201903523YA (en) * | 2016-10-20 | 2019-05-30 | Harvard College | In vitro and cell based assays for measuring the activity of botulinum neurotoxins |
-
2017
- 2017-05-16 WO PCT/US2017/032985 patent/WO2017201105A1/en unknown
- 2017-05-16 US US16/302,088 patent/US11242515B2/en active Active
- 2017-05-16 UA UAA201812420A patent/UA127310C2/uk unknown
- 2017-05-16 BR BR112018073591A patent/BR112018073591A2/pt unknown
- 2017-05-16 MX MX2018014066A patent/MX2018014066A/es unknown
- 2017-05-16 CA CA3024331A patent/CA3024331A1/en active Pending
- 2017-05-16 JP JP2018560214A patent/JP7060522B2/ja active Active
- 2017-05-16 CN CN201780030496.9A patent/CN109641941B/zh active Active
- 2017-05-16 KR KR1020187033716A patent/KR102567311B1/ko active IP Right Grant
- 2017-05-16 AU AU2017268285A patent/AU2017268285B2/en active Active
- 2017-05-16 SG SG11201809903WA patent/SG11201809903WA/en unknown
- 2017-05-16 EP EP17728308.2A patent/EP3458472B1/en active Active
- 2017-05-16 ES ES17728308T patent/ES2904269T3/es active Active
- 2017-05-16 EA EA201892643A patent/EA201892643A1/ru unknown
-
2018
- 2018-11-13 ZA ZA2018/07626A patent/ZA201807626B/en unknown
- 2018-11-15 SA SA518400443A patent/SA518400443B1/ar unknown
Also Published As
| Publication number | Publication date |
|---|---|
| ES2904269T3 (es) | 2022-04-04 |
| AU2017268285A1 (en) | 2018-11-29 |
| JP7060522B2 (ja) | 2022-04-26 |
| AU2017268285B2 (en) | 2021-07-29 |
| EP3458472A1 (en) | 2019-03-27 |
| SA518400443B1 (ar) | 2022-06-22 |
| US11242515B2 (en) | 2022-02-08 |
| US20190153418A1 (en) | 2019-05-23 |
| ZA201807626B (en) | 2023-10-25 |
| CN109641941A (zh) | 2019-04-16 |
| KR102567311B1 (ko) | 2023-08-16 |
| UA127310C2 (uk) | 2023-07-19 |
| KR20190015240A (ko) | 2019-02-13 |
| EA201892643A1 (ru) | 2019-06-28 |
| BR112018073591A2 (pt) | 2019-03-19 |
| EP3458472B1 (en) | 2021-10-20 |
| CN109641941B (zh) | 2023-06-16 |
| SG11201809903WA (en) | 2018-12-28 |
| CA3024331A1 (en) | 2017-11-23 |
| WO2017201105A1 (en) | 2017-11-23 |
| JP2019518447A (ja) | 2019-07-04 |
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