RU2712562C2 - Модулирование клеточного роста и гликозилирования при производстве рекомбинантных гликопротеинов - Google Patents
Модулирование клеточного роста и гликозилирования при производстве рекомбинантных гликопротеинов Download PDFInfo
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- RU2712562C2 RU2712562C2 RU2016138176A RU2016138176A RU2712562C2 RU 2712562 C2 RU2712562 C2 RU 2712562C2 RU 2016138176 A RU2016138176 A RU 2016138176A RU 2016138176 A RU2016138176 A RU 2016138176A RU 2712562 C2 RU2712562 C2 RU 2712562C2
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- zinc
- manganese
- copper
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- iron
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
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- C07—ORGANIC CHEMISTRY
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
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- C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
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| WO2013078106A1 (en) | 2011-11-21 | 2013-05-30 | Nonomura Arthur M | Methods and systems for growing plants using silicate-based substrates, cultivation of enhanced photosynthetic productivity and photosafening by utilization of exogenous glycopyranosides for endogenous glycopyranosyl-protein derivatives, and formulations, processes and systems for the same |
| SI3110961T1 (sl) * | 2014-02-27 | 2020-03-31 | F. Hoffmann-La Roche Ag | Modulacija rasti celic in glikozilacije pri rekombinantni proizvodnji glikoproteina |
| AU2015355087C1 (en) * | 2014-12-01 | 2025-10-30 | Amgen Inc. | Process for manipulating the level of glycan content of a glycoprotein |
| US11013234B2 (en) * | 2016-04-29 | 2021-05-25 | Innovation Hammer Llc | Formulations and methods for treating photosynthetic organisms and enhancing qualities and quantities of yields with glycan composite formulations |
| US12503700B2 (en) | 2017-03-14 | 2025-12-23 | Amgen Inc. | Control of total afucosylated glycoforms of antibodies produced in cell culture |
| WO2019077628A1 (en) * | 2017-10-16 | 2019-04-25 | Council Of Scientific & Industrial Research | ZINC SUPPLEMENTATION TO DECREASE GALACTOSYLATION OF RECOMBINANT GLYCOPROTEINS |
| KR20250016476A (ko) * | 2018-03-16 | 2025-02-03 | 젠자임 코포레이션 | 생산용 생물 반응기에서의 세포 생존력을 개선하는 방법 |
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| CA3099917A1 (en) * | 2018-05-24 | 2019-11-28 | Ares Trading S.A. | Method for controlling the afucosylation level of a glycoprotein composition |
| HU231514B1 (hu) | 2018-11-07 | 2024-07-28 | Richter Gedeon Nyrt. | Sejttenyészetben előállított rekombináns glikoprotein glikozilációs mintázatának megváltoztatására szolgáló módszer |
| US11634499B2 (en) * | 2018-11-13 | 2023-04-25 | Janssen Biotech, Inc. | Control of trace metals during production of anti-CD38 antibodies |
| JP2020188737A (ja) * | 2019-05-23 | 2020-11-26 | 東ソー株式会社 | 抗体依存性細胞傷害活性が向上した抗体の製造方法 |
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| WO1996039488A1 (en) * | 1995-06-06 | 1996-12-12 | Genentech, Inc. | Process for controlling sialylation of proteins produced by mammalian cell culture |
| EP1096017A2 (en) * | 1999-10-26 | 2001-05-02 | Welfide Corporation | Method for increasing the production of physiologically active substances by cultured cells |
| US20050287666A1 (en) * | 2004-06-29 | 2005-12-29 | Invitrogen Corporation | Cell culture medium comprising transition metals or trace elements |
| RU2463345C2 (ru) * | 2006-07-13 | 2012-10-10 | Вайет | Получение гликопротеинов |
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| US4767704A (en) | 1983-10-07 | 1988-08-30 | Columbia University In The City Of New York | Protein-free culture medium |
| IL87737A (en) | 1987-09-11 | 1993-08-18 | Genentech Inc | Method for culturing polypeptide factor dependent vertebrate recombinant cells |
| US6030945A (en) | 1996-01-09 | 2000-02-29 | Genentech, Inc. | Apo-2 ligand |
| EP2218775B1 (en) | 1996-08-30 | 2015-01-28 | Life Technologies Corporation | Method for producing a polypeptide in vitro in mammalian cells in a protein-free and serum-free culture medium |
| DE69929198T2 (de) * | 1998-05-29 | 2006-08-10 | Genentech, Inc., South San Francisco | Zellkulturverfahren für die produktion von glycoproteinen |
| DE60230858D1 (de) | 2001-02-15 | 2009-03-05 | Centocor Inc | Chemisch definiertes medium für kultivierte säugerzellen |
| TWI384069B (zh) | 2004-08-27 | 2013-02-01 | Pfizer Ireland Pharmaceuticals | 多胜肽之製法 |
| ES2361559T3 (es) | 2004-11-02 | 2011-06-20 | Ares Trading S.A. | Medio de cultivo de células exento de suero para mamíferos. |
| US20070231895A1 (en) | 2005-11-02 | 2007-10-04 | Lee Gene W | Methods for adapting mammalian cells |
| EP2495308A1 (en) | 2005-12-08 | 2012-09-05 | Amgen Inc. | Improved production of glycoproteins using manganese |
| US8192951B2 (en) | 2006-11-03 | 2012-06-05 | Wyeth Llc | Glycolysis-inhibiting substances in cell culture |
| EP2924113B1 (en) | 2007-03-02 | 2019-04-10 | Wyeth LLC | Use of copper and glutamate in cell culture for production of polypeptides |
| US20110053223A1 (en) * | 2009-08-14 | 2011-03-03 | Robert Bayer | Cell culture methods to make antibodies with enhanced adcc function |
| US20120309056A1 (en) * | 2010-02-04 | 2012-12-06 | Leon Arnaud | Fed-batch process using concentrated cell culture medium for the efficient production of biologics in eb66 cells |
| CN102858954A (zh) | 2010-04-26 | 2013-01-02 | 诺瓦提斯公司 | 改进的细胞培养方法 |
| US9062106B2 (en) | 2011-04-27 | 2015-06-23 | Abbvie Inc. | Methods for controlling the galactosylation profile of recombinantly-expressed proteins |
| CN105189735B (zh) | 2011-04-29 | 2019-04-12 | 拜康研究有限公司 | 在培养期间减少乳酸累积的方法和生产多肽的方法 |
| US10059770B2 (en) | 2012-01-30 | 2018-08-28 | Dr. Reddy's Laboratories Limited | Process of modulating man5 and/or afucosylation content of a glycoprotein composition |
| EP3052640A2 (en) | 2013-10-04 | 2016-08-10 | AbbVie Inc. | Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins |
| SI3110961T1 (sl) * | 2014-02-27 | 2020-03-31 | F. Hoffmann-La Roche Ag | Modulacija rasti celic in glikozilacije pri rekombinantni proizvodnji glikoproteina |
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- 2015-02-24 BR BR112016017660-0A patent/BR112016017660B1/pt active IP Right Grant
- 2015-02-24 MX MX2016010236A patent/MX369395B/es active IP Right Grant
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- 2015-02-24 WO PCT/EP2015/053804 patent/WO2015128314A1/en not_active Ceased
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- 2015-02-24 KR KR1020167026630A patent/KR102280638B1/ko active Active
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Patent Citations (4)
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| WO1996039488A1 (en) * | 1995-06-06 | 1996-12-12 | Genentech, Inc. | Process for controlling sialylation of proteins produced by mammalian cell culture |
| EP1096017A2 (en) * | 1999-10-26 | 2001-05-02 | Welfide Corporation | Method for increasing the production of physiologically active substances by cultured cells |
| US20050287666A1 (en) * | 2004-06-29 | 2005-12-29 | Invitrogen Corporation | Cell culture medium comprising transition metals or trace elements |
| RU2463345C2 (ru) * | 2006-07-13 | 2012-10-10 | Вайет | Получение гликопротеинов |
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| GAWLITZEK M. Estacio M, Furch T, Kiss R. Identification of cell culture conditions to control N-glycosylation site-occupancy of recombinant glycoproteins expressed in CHO cells. Biotechnol Bioeng. 2009;103(6):1164-1175. * |
| HAN Y. et al. "Cultivation of Recombinant Chinese hamster ovary cells grown as suspended aggregates in stirred vessels". Journal of Bioscience and Bioengineering, 2006, 102(5), 430-435. doi:10.1263/jbb.102.430. * |
| P. HOSSLER et al. "Optimal and consistent protein glycosylation in mammalian cell culture". Glycobiology, 2009, v.19, no.9, p.936-949, https://doi.org/10.1093/glycob/cwp079. * |
| RAYNER M. et al. "A simple and effective method for the removal of trace metal cations from a mammalian culture medium supplemented with 10% fetal calf serum". Biometals, 1995, 8(3), p.188-192. doi:10.1007/bf00143374. * |
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| BR112016017660B1 (pt) | 2022-04-19 |
| DK3110961T3 (da) | 2020-02-03 |
| MX2016010236A (es) | 2016-10-13 |
| RS59881B1 (sr) | 2020-03-31 |
| KR102280638B1 (ko) | 2021-07-22 |
| CA2937611A1 (en) | 2015-09-03 |
| CN106133146B (zh) | 2021-05-04 |
| SI3110961T1 (sl) | 2020-03-31 |
| US20170058309A1 (en) | 2017-03-02 |
| BR112016017660A2 (enExample) | 2017-08-08 |
| CN106133146A (zh) | 2016-11-16 |
| RU2016138176A3 (enExample) | 2018-10-29 |
| MX369395B (es) | 2019-11-07 |
| WO2015128314A1 (en) | 2015-09-03 |
| JP6934507B2 (ja) | 2021-09-15 |
| KR20160125514A (ko) | 2016-10-31 |
| CA2937611C (en) | 2023-01-03 |
| LT3110961T (lt) | 2020-02-10 |
| HUE047575T2 (hu) | 2020-04-28 |
| JP6831702B2 (ja) | 2021-02-17 |
| EP3110961B1 (en) | 2019-11-27 |
| JP2017506515A (ja) | 2017-03-09 |
| US20210222220A1 (en) | 2021-07-22 |
| EP3110961A1 (en) | 2017-01-04 |
| ES2769003T3 (es) | 2020-06-24 |
| US20240327890A1 (en) | 2024-10-03 |
| PL3110961T3 (pl) | 2020-04-30 |
| RU2016138176A (ru) | 2018-03-29 |
| HRP20200100T1 (hr) | 2020-04-03 |
| SG11201606856SA (en) | 2016-09-29 |
| JP2020054351A (ja) | 2020-04-09 |
| PT3110961T (pt) | 2020-01-29 |
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