BR112016017660B1 - Método para a produção de uma glicoproteína recombinante - Google Patents

Método para a produção de uma glicoproteína recombinante Download PDF

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Publication number
BR112016017660B1
BR112016017660B1 BR112016017660-0A BR112016017660A BR112016017660B1 BR 112016017660 B1 BR112016017660 B1 BR 112016017660B1 BR 112016017660 A BR112016017660 A BR 112016017660A BR 112016017660 B1 BR112016017660 B1 BR 112016017660B1
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BR
Brazil
Prior art keywords
manganese
zinc
copper
iron
glycoprotein
Prior art date
Application number
BR112016017660-0A
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English (en)
Portuguese (pt)
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BR112016017660A2 (enExample
Inventor
Oliver Popp
Nicola Beaucamp
Georg Drabner
Stephanie Esslinger
Original Assignee
F. Hoffmann-La Roche Ag
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=50184787&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BR112016017660(B1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by F. Hoffmann-La Roche Ag filed Critical F. Hoffmann-La Roche Ag
Publication of BR112016017660A2 publication Critical patent/BR112016017660A2/pt
Publication of BR112016017660B1 publication Critical patent/BR112016017660B1/pt

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018Culture media for cell or tissue culture
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/005Glycopeptides, glycoproteins
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/40Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41Glycosylation, sialylation, or fucosylation

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
BR112016017660-0A 2014-02-27 2015-02-24 Método para a produção de uma glicoproteína recombinante BR112016017660B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP14157030.9 2014-02-27
EP14157030 2014-02-27
PCT/EP2015/053804 WO2015128314A1 (en) 2014-02-27 2015-02-24 Modulation of cell growth and glycosylation in recombinant glycoprotein production

Publications (2)

Publication Number Publication Date
BR112016017660A2 BR112016017660A2 (enExample) 2017-08-08
BR112016017660B1 true BR112016017660B1 (pt) 2022-04-19

Family

ID=50184787

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112016017660-0A BR112016017660B1 (pt) 2014-02-27 2015-02-24 Método para a produção de uma glicoproteína recombinante

Country Status (20)

Country Link
US (3) US20170058309A1 (enExample)
EP (1) EP3110961B1 (enExample)
JP (2) JP6831702B2 (enExample)
KR (1) KR102280638B1 (enExample)
CN (1) CN106133146B (enExample)
BR (1) BR112016017660B1 (enExample)
CA (1) CA2937611C (enExample)
DK (1) DK3110961T3 (enExample)
ES (1) ES2769003T3 (enExample)
HR (1) HRP20200100T1 (enExample)
HU (1) HUE047575T2 (enExample)
LT (1) LT3110961T (enExample)
MX (1) MX369395B (enExample)
PL (1) PL3110961T3 (enExample)
PT (1) PT3110961T (enExample)
RS (1) RS59881B1 (enExample)
RU (1) RU2712562C2 (enExample)
SG (1) SG11201606856SA (enExample)
SI (1) SI3110961T1 (enExample)
WO (1) WO2015128314A1 (enExample)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013078106A1 (en) 2011-11-21 2013-05-30 Nonomura Arthur M Methods and systems for growing plants using silicate-based substrates, cultivation of enhanced photosynthetic productivity and photosafening by utilization of exogenous glycopyranosides for endogenous glycopyranosyl-protein derivatives, and formulations, processes and systems for the same
SI3110961T1 (sl) * 2014-02-27 2020-03-31 F. Hoffmann-La Roche Ag Modulacija rasti celic in glikozilacije pri rekombinantni proizvodnji glikoproteina
AU2015355087C1 (en) * 2014-12-01 2025-10-30 Amgen Inc. Process for manipulating the level of glycan content of a glycoprotein
US11013234B2 (en) * 2016-04-29 2021-05-25 Innovation Hammer Llc Formulations and methods for treating photosynthetic organisms and enhancing qualities and quantities of yields with glycan composite formulations
US12503700B2 (en) 2017-03-14 2025-12-23 Amgen Inc. Control of total afucosylated glycoforms of antibodies produced in cell culture
WO2019077628A1 (en) * 2017-10-16 2019-04-25 Council Of Scientific & Industrial Research ZINC SUPPLEMENTATION TO DECREASE GALACTOSYLATION OF RECOMBINANT GLYCOPROTEINS
KR20250016476A (ko) * 2018-03-16 2025-02-03 젠자임 코포레이션 생산용 생물 반응기에서의 세포 생존력을 개선하는 방법
SG11202009216YA (en) 2018-03-26 2020-10-29 Amgen Inc Total afucosylated glycoforms of antibodies produced in cell culture
CA3099917A1 (en) * 2018-05-24 2019-11-28 Ares Trading S.A. Method for controlling the afucosylation level of a glycoprotein composition
HU231514B1 (hu) 2018-11-07 2024-07-28 Richter Gedeon Nyrt. Sejttenyészetben előállított rekombináns glikoprotein glikozilációs mintázatának megváltoztatására szolgáló módszer
US11634499B2 (en) * 2018-11-13 2023-04-25 Janssen Biotech, Inc. Control of trace metals during production of anti-CD38 antibodies
JP2020188737A (ja) * 2019-05-23 2020-11-26 東ソー株式会社 抗体依存性細胞傷害活性が向上した抗体の製造方法
CR20220531A (es) * 2020-04-24 2022-11-28 Hoffmann La Roche Modulacion de enzimas y vías con compuestos de sulfhidrilo y sus derivados
WO2025036924A1 (en) * 2023-08-16 2025-02-20 Glaxosmithkline Intellectual Property Limited Method of controlling antibody glycosylation profile

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4767704A (en) 1983-10-07 1988-08-30 Columbia University In The City Of New York Protein-free culture medium
IL87737A (en) 1987-09-11 1993-08-18 Genentech Inc Method for culturing polypeptide factor dependent vertebrate recombinant cells
US5705364A (en) * 1995-06-06 1998-01-06 Genentech, Inc. Mammalian cell culture process
US6030945A (en) 1996-01-09 2000-02-29 Genentech, Inc. Apo-2 ligand
EP2218775B1 (en) 1996-08-30 2015-01-28 Life Technologies Corporation Method for producing a polypeptide in vitro in mammalian cells in a protein-free and serum-free culture medium
DE69929198T2 (de) * 1998-05-29 2006-08-10 Genentech, Inc., South San Francisco Zellkulturverfahren für die produktion von glycoproteinen
JP2001120262A (ja) 1999-10-26 2001-05-08 Welfide Corp 生理活性物質の産生増強方法
DE60230858D1 (de) 2001-02-15 2009-03-05 Centocor Inc Chemisch definiertes medium für kultivierte säugerzellen
US20050287666A1 (en) * 2004-06-29 2005-12-29 Invitrogen Corporation Cell culture medium comprising transition metals or trace elements
TWI384069B (zh) 2004-08-27 2013-02-01 Pfizer Ireland Pharmaceuticals 多胜肽之製法
ES2361559T3 (es) 2004-11-02 2011-06-20 Ares Trading S.A. Medio de cultivo de células exento de suero para mamíferos.
US20070231895A1 (en) 2005-11-02 2007-10-04 Lee Gene W Methods for adapting mammalian cells
EP2495308A1 (en) 2005-12-08 2012-09-05 Amgen Inc. Improved production of glycoproteins using manganese
ES2440487T3 (es) 2006-07-13 2014-01-29 Wyeth Llc Producción de glucoproteínas
US8192951B2 (en) 2006-11-03 2012-06-05 Wyeth Llc Glycolysis-inhibiting substances in cell culture
EP2924113B1 (en) 2007-03-02 2019-04-10 Wyeth LLC Use of copper and glutamate in cell culture for production of polypeptides
US20110053223A1 (en) * 2009-08-14 2011-03-03 Robert Bayer Cell culture methods to make antibodies with enhanced adcc function
US20120309056A1 (en) * 2010-02-04 2012-12-06 Leon Arnaud Fed-batch process using concentrated cell culture medium for the efficient production of biologics in eb66 cells
CN102858954A (zh) 2010-04-26 2013-01-02 诺瓦提斯公司 改进的细胞培养方法
US9062106B2 (en) 2011-04-27 2015-06-23 Abbvie Inc. Methods for controlling the galactosylation profile of recombinantly-expressed proteins
CN105189735B (zh) 2011-04-29 2019-04-12 拜康研究有限公司 在培养期间减少乳酸累积的方法和生产多肽的方法
US10059770B2 (en) 2012-01-30 2018-08-28 Dr. Reddy's Laboratories Limited Process of modulating man5 and/or afucosylation content of a glycoprotein composition
EP3052640A2 (en) 2013-10-04 2016-08-10 AbbVie Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
SI3110961T1 (sl) * 2014-02-27 2020-03-31 F. Hoffmann-La Roche Ag Modulacija rasti celic in glikozilacije pri rekombinantni proizvodnji glikoproteina

Also Published As

Publication number Publication date
DK3110961T3 (da) 2020-02-03
MX2016010236A (es) 2016-10-13
RS59881B1 (sr) 2020-03-31
KR102280638B1 (ko) 2021-07-22
CA2937611A1 (en) 2015-09-03
CN106133146B (zh) 2021-05-04
SI3110961T1 (sl) 2020-03-31
US20170058309A1 (en) 2017-03-02
BR112016017660A2 (enExample) 2017-08-08
CN106133146A (zh) 2016-11-16
RU2016138176A3 (enExample) 2018-10-29
MX369395B (es) 2019-11-07
WO2015128314A1 (en) 2015-09-03
JP6934507B2 (ja) 2021-09-15
KR20160125514A (ko) 2016-10-31
CA2937611C (en) 2023-01-03
LT3110961T (lt) 2020-02-10
HUE047575T2 (hu) 2020-04-28
JP6831702B2 (ja) 2021-02-17
EP3110961B1 (en) 2019-11-27
JP2017506515A (ja) 2017-03-09
US20210222220A1 (en) 2021-07-22
EP3110961A1 (en) 2017-01-04
ES2769003T3 (es) 2020-06-24
RU2712562C2 (ru) 2020-01-29
US20240327890A1 (en) 2024-10-03
PL3110961T3 (pl) 2020-04-30
RU2016138176A (ru) 2018-03-29
HRP20200100T1 (hr) 2020-04-03
SG11201606856SA (en) 2016-09-29
JP2020054351A (ja) 2020-04-09
PT3110961T (pt) 2020-01-29

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B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]

Free format text: DE ACORDO COM O ARTIGO 229-C DA LEI NO 10196/2001, QUE MODIFICOU A LEI NO 9279/96, A CONCESSAO DA PATENTE ESTA CONDICIONADA A ANUENCIA PREVIA DA ANVISA. CONSIDERANDO A APROVACAO DOS TERMOS DO PARECER NO 337/PGF/EA/2010, BEM COMO A PORTARIA INTERMINISTERIAL NO 1065 DE 24/05/2012, ENCAMINHA-SE O PRESENTE PEDIDO PARA AS PROVIDENCIAS CABIVEIS.

B07E Notification of approval relating to section 229 industrial property law [chapter 7.5 patent gazette]
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B09X Republication of the decision to grant [chapter 9.1.3 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 24/02/2015, OBSERVADAS AS CONDICOES LEGAIS.