RU2019102714A - Инсектицидные белки из растений и способы их применения - Google Patents
Инсектицидные белки из растений и способы их применения Download PDFInfo
- Publication number
- RU2019102714A RU2019102714A RU2019102714A RU2019102714A RU2019102714A RU 2019102714 A RU2019102714 A RU 2019102714A RU 2019102714 A RU2019102714 A RU 2019102714A RU 2019102714 A RU2019102714 A RU 2019102714A RU 2019102714 A RU2019102714 A RU 2019102714A
- Authority
- RU
- Russia
- Prior art keywords
- recombinant
- pest
- insecticidal polypeptide
- polynucleotide
- transgenic plant
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/50—Isolated enzymes; Isolated proteins
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Claims (16)
1. Рекомбинантный инсектицидный полипептид, содержащий аминокислотную последовательность, характеризующуюся по меньшей мере 60% идентичностью последовательности с SEQ ID NO: 2.
2. Рекомбинантный инсектицидный полипептид по п. 1, где аминокислотная последовательность характеризуется по меньшей мере 70% идентичностью последовательности с SEQ ID NO: 2.
3. Рекомбинантный инсектицидный полипептид по п. 1, где аминокислотная последовательность характеризуется по меньшей мере 80% идентичностью последовательности с SEQ ID NO: 2.
4. Рекомбинантный инсектицидный полипептид по любому из пп. 1, 2 или 3, где инсектицидный полипептид соединен с гетерологичной сигнальной последовательностью или транзитной последовательностью.
5. Композиция, содержащая по меньшей мере один рекомбинантный инсектицидный полипептид по пп. 1, 2, 3 или 4.
6. Рекомбинантный полинуклеотид, кодирующий инсектицидный полипептид по пп. 1, 2, 3 или 4.
7. Рекомбинантный полинуклеотид по п. 6, где полинуклеотид содержит кодоны, оптимизированные для экспрессии в культуре, важной с точки зрения сельского хозяйства.
8. Рекомбинантный полинуклеотид по п. 6, где полинуклеотид представляет собой кДНК.
9. ДНК-конструкция, содержащая рекомбинантный полинуклеотид по любому из пп. 6, 7 или 8, функционально связанный с гетерологичным регуляторным элементом.
10. Трансгенное растение, содержащее полинуклеотид по любому из пп. 6, 7 или 8.
11. Трансгенное растение, содержащее ДНК-конструкцию по п. 9.
12. Способ подавления роста или уничтожения насекомого-вредителя или популяции вредителя, предусматривающий приведение насекомого-вредителя в контакт с инсектицидным полипептидом по пп. 1, 2, 3 или 4.
13. Способ подавления роста или уничтожения насекомого-вредителя или популяции вредителя, предусматривающий обеспечение экспрессии в растении полинуклеотида по пп. 1, 2, 3 или 4.
14. Способ контроля заражения вредителем, предусматривающий обеспечение наличия в рационе вредителя трансгенного растения по п. 11 или его части.
15. Способ улучшения урожайности сельскохозяйственной культуры, предусматривающий выращивание трансгенного растения по п. 11, где урожайность сельскохозяйственной культуры в присутствии насекомого-вредителя увеличивается по сравнению с сельскохозяйственной культурой, не содержащей указанное трансгенное растение.
16. Способ по любому из пп. 12, 13, 14 или 15, где насекомое-вредитель или популяция вредителя являются устойчивыми к по меньшей мере одному инсектицидному белку Cry.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662357501P | 2016-07-01 | 2016-07-01 | |
| US62/357,501 | 2016-07-01 | ||
| PCT/US2017/039376 WO2018005411A1 (en) | 2016-07-01 | 2017-06-27 | Insecticidal proteins from plants and methods for their use |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| RU2019102714A true RU2019102714A (ru) | 2020-08-03 |
Family
ID=60786944
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2019102714A RU2019102714A (ru) | 2016-07-01 | 2017-06-27 | Инсектицидные белки из растений и способы их применения |
Country Status (11)
| Country | Link |
|---|---|
| US (4) | US11155829B2 (ru) |
| EP (2) | EP3954202A1 (ru) |
| CN (1) | CN109788735A (ru) |
| AR (1) | AR110614A1 (ru) |
| BR (1) | BR112018077339A2 (ru) |
| CA (1) | CA3026113A1 (ru) |
| MX (2) | MX2018015906A (ru) |
| PH (1) | PH12018502706A1 (ru) |
| RU (1) | RU2019102714A (ru) |
| WO (1) | WO2018005411A1 (ru) |
| ZA (1) | ZA201807971B (ru) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA3083276A1 (en) * | 2017-12-22 | 2019-06-27 | Pioneer Hi-Bred International, Inc. | Combinations of insecticidal polypeptides having improved activity spectrum and uses thereof |
| CA3092078A1 (en) * | 2018-03-14 | 2019-09-19 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins from plants and methods for their use |
| CN116410286A (zh) | 2018-03-14 | 2023-07-11 | 先锋国际良种公司 | 来自植物的杀昆虫蛋白及其使用方法 |
| WO2021076346A1 (en) | 2019-10-18 | 2021-04-22 | Pioneer Hi-Bred International, Inc. | Maize event dp-202216-6 and dp-023211-2 stack |
| TW202142114A (zh) | 2020-02-04 | 2021-11-16 | 美商陶氏農業科學公司 | 具有殺有害生物效用之組成物及與其相關之方法 |
| CA3186978A1 (en) | 2020-07-14 | 2022-01-20 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| CN112243632B (zh) * | 2020-10-15 | 2022-04-01 | 上海市农业科学院 | 一种陈花菜类种子的处理方法及其应用 |
| CN113545369B (zh) * | 2021-08-23 | 2022-09-09 | 江西劲农作物保护有限公司 | 一种用于防治番茄白粉虱的农药组合物 |
| TW202345696A (zh) | 2022-05-18 | 2023-12-01 | 美商科迪華農業科技有限責任公司 | 具有殺有害生物效用之組成物及與其相關的方法 |
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| AU2012360018B2 (en) * | 2011-12-28 | 2015-10-01 | Council Of Scientific & Industrial Research | A novel insecticidal chitinase protein its encoding nucleotide and application thereof |
| US9688730B2 (en) | 2012-07-02 | 2017-06-27 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| US9475847B2 (en) | 2012-07-26 | 2016-10-25 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| US9920316B2 (en) | 2013-03-14 | 2018-03-20 | Pioneer Hi-Bred International, Inc. | Compositions and methods to control insect pests |
| US9394345B2 (en) | 2013-03-15 | 2016-07-19 | Pioneer Hi-Bred International, Inc. | PHI-4 polypeptides and methods for their use |
| WO2014150914A2 (en) | 2013-03-15 | 2014-09-25 | Pioneer Hi-Bred International, Inc. | Phi-4 polypeptides and methods for their use |
| US10006045B2 (en) | 2013-08-16 | 2018-06-26 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| CA3175967A1 (en) | 2013-09-13 | 2015-03-19 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| WO2015120270A1 (en) | 2014-02-07 | 2015-08-13 | Pioneer Hi Bred International, Inc. | Insecticidal proteins and methods for their use |
| UA120608C2 (uk) | 2014-02-07 | 2020-01-10 | Піонір Хай-Бред Інтернешнл, Інк. | Очищений поліпептид ptip-83 та спосіб його застосування |
| US20150257389A1 (en) | 2014-03-14 | 2015-09-17 | Pioneer Hi-Bred International, Inc. | Compositions and methods to control insect pests |
| CA2963550A1 (en) * | 2014-10-16 | 2016-04-21 | Pioneer Hi-Bred International, Inc. | Insecticidal polypeptides having broad spectrum activity and uses thereof |
| RU2017116800A (ru) * | 2014-10-16 | 2018-11-19 | Пайонир Хай-Бред Интернэшнл, Инк. | Инсектицидные полипептиды с широким спектром активности и их применения |
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2017
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2018
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| EP3478052B1 (en) | 2021-08-25 |
| EP3478052A1 (en) | 2019-05-08 |
| US20240076688A1 (en) | 2024-03-07 |
| EP3478052A4 (en) | 2019-12-04 |
| EP3954202A1 (en) | 2022-02-16 |
| BR112018077339A2 (pt) | 2019-04-24 |
| ZA201807971B (en) | 2019-06-26 |
| MX2018015906A (es) | 2019-04-04 |
| WO2018005411A1 (en) | 2018-01-04 |
| US20220002747A1 (en) | 2022-01-06 |
| CN109788735A (zh) | 2019-05-21 |
| PH12018502706A1 (en) | 2019-11-11 |
| MX2021013184A (es) | 2022-01-06 |
| AR110614A1 (es) | 2019-04-17 |
| CA3026113A1 (en) | 2018-01-04 |
| US11155829B2 (en) | 2021-10-26 |
| US20190211352A1 (en) | 2019-07-11 |
| US20220010329A1 (en) | 2022-01-13 |
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