RU2015137158A - МУТЕИНЫ ЛИПОКАЛИНА 2 ЧЕЛОВЕКА (Lcn2,hNGAL) С АФФИННОСТЬЮ ДЛЯ ОПРЕДЕЛЕННОЙ МИШЕНИ - Google Patents
МУТЕИНЫ ЛИПОКАЛИНА 2 ЧЕЛОВЕКА (Lcn2,hNGAL) С АФФИННОСТЬЮ ДЛЯ ОПРЕДЕЛЕННОЙ МИШЕНИ Download PDFInfo
- Publication number
- RU2015137158A RU2015137158A RU2015137158A RU2015137158A RU2015137158A RU 2015137158 A RU2015137158 A RU 2015137158A RU 2015137158 A RU2015137158 A RU 2015137158A RU 2015137158 A RU2015137158 A RU 2015137158A RU 2015137158 A RU2015137158 A RU 2015137158A
- Authority
- RU
- Russia
- Prior art keywords
- mutein
- arg
- lcn2
- amino acid
- target
- Prior art date
Links
- 101150055061 LCN2 gene Proteins 0.000 title claims 8
- 108090000765 processed proteins & peptides Proteins 0.000 claims 8
- 125000003275 alpha amino acid group Chemical group 0.000 claims 6
- 102000039446 nucleic acids Human genes 0.000 claims 5
- 108020004707 nucleic acids Proteins 0.000 claims 5
- 150000007523 nucleic acids Chemical class 0.000 claims 5
- 229920001184 polypeptide Polymers 0.000 claims 4
- 102000004196 processed proteins & peptides Human genes 0.000 claims 4
- 102000004169 proteins and genes Human genes 0.000 claims 4
- 108090000623 proteins and genes Proteins 0.000 claims 4
- 125000000539 amino acid group Chemical group 0.000 claims 3
- 238000000034 method Methods 0.000 claims 3
- 239000002773 nucleotide Substances 0.000 claims 3
- 125000003729 nucleotide group Chemical group 0.000 claims 3
- 238000006467 substitution reaction Methods 0.000 claims 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical class N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims 2
- 108010090849 Amyloid beta-Peptides Proteins 0.000 claims 2
- 102000013455 Amyloid beta-Peptides Human genes 0.000 claims 2
- 102000016359 Fibronectins Human genes 0.000 claims 2
- 108010067306 Fibronectins Proteins 0.000 claims 2
- 101001023833 Homo sapiens Neutrophil gelatinase-associated lipocalin Proteins 0.000 claims 2
- 210000004027 cell Anatomy 0.000 claims 2
- 150000001875 compounds Chemical class 0.000 claims 2
- 239000012634 fragment Substances 0.000 claims 2
- 102000047202 human LCN2 Human genes 0.000 claims 2
- 239000002184 metal Chemical class 0.000 claims 2
- 229910052751 metal Inorganic materials 0.000 claims 2
- 230000004962 physiological condition Effects 0.000 claims 2
- 238000009007 Diagnostic Kit Methods 0.000 claims 1
- SHIBSTMRCDJXLN-UHFFFAOYSA-N Digoxigenin Natural products C1CC(C2C(C3(C)CCC(O)CC3CC2)CC2O)(O)C2(C)C1C1=CC(=O)OC1 SHIBSTMRCDJXLN-UHFFFAOYSA-N 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 claims 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 claims 1
- 108010064539 amyloid beta-protein (1-42) Proteins 0.000 claims 1
- 239000011616 biotin Chemical class 0.000 claims 1
- 229960002685 biotin Drugs 0.000 claims 1
- 235000020958 biotin Nutrition 0.000 claims 1
- 210000004899 c-terminal region Anatomy 0.000 claims 1
- 102220515765 cAMP-dependent protein kinase inhibitor alpha_N10D_mutation Human genes 0.000 claims 1
- 230000001268 conjugating effect Effects 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 239000000824 cytostatic agent Chemical class 0.000 claims 1
- QONQRTHLHBTMGP-UHFFFAOYSA-N digitoxigenin Natural products CC12CCC(C3(CCC(O)CC3CC3)C)C3C11OC1CC2C1=CC(=O)OC1 QONQRTHLHBTMGP-UHFFFAOYSA-N 0.000 claims 1
- SHIBSTMRCDJXLN-KCZCNTNESA-N digoxigenin Chemical class C1([C@@H]2[C@@]3([C@@](CC2)(O)[C@H]2[C@@H]([C@@]4(C)CC[C@H](O)C[C@H]4CC2)C[C@H]3O)C)=CC(=O)OC1 SHIBSTMRCDJXLN-KCZCNTNESA-N 0.000 claims 1
- 230000004927 fusion Effects 0.000 claims 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims 1
- 238000002955 isolation Methods 0.000 claims 1
- 150000002739 metals Chemical class 0.000 claims 1
- 238000002703 mutagenesis Methods 0.000 claims 1
- 231100000350 mutagenesis Toxicity 0.000 claims 1
- 108020001580 protein domains Proteins 0.000 claims 1
- 230000002285 radioactive effect Effects 0.000 claims 1
- 230000000405 serological effect Effects 0.000 claims 1
- 239000003053 toxin Chemical class 0.000 claims 1
- 231100000765 toxin Toxicity 0.000 claims 1
- 108700012359 toxins Chemical class 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/1703—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- A61K38/1709—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/56—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule
- A61K47/59—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes
- A61K47/60—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes the organic macromolecular compound being a polyoxyalkylene oligomer, polymer or dendrimer, e.g. PEG, PPG, PEO or polyglycerol
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/775—Apolipopeptides
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2795/00—Bacteriophages
- C12N2795/00011—Details
- C12N2795/00041—Use of virus, viral particle or viral elements as a vector
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biomedical Technology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Toxicology (AREA)
- Epidemiology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biotechnology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Neurosurgery (AREA)
- Neurology (AREA)
- Food Science & Technology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Analytical Chemistry (AREA)
- Wood Science & Technology (AREA)
Claims (23)
1. Способ продуцирования мутеина из липокалина 2 человека (Lcn2, hNGAL), при котором указанный мутеин связывается с заранее определенной мишенью с обнаруживаемой аффинностью, не связывающуюся с нативным зрелым hNGAL в физиологических условиях, включающий:
(a) мутагенез молекулы нуклеиновой кислоты, кодирующей белок Lcn2 человека, по меньшей мере, в кодирующем триплете нуклеотидов в позициях 100 и/или 106 линейной полипептидной последовательности Lcn2 человека и по меньшей мере в 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17 или 18 триплетах нуклеотидов, кодирующих любую из позиций 36, 40, 41, 49, 52, 68, 70, 72, 73, 77, 79, 81, 96, 103, 125, 127, 132 и 134 линейной полипептидной последовательности Lcn2 человека с получением в результате одной или более молекул нуклеиновой кислоты мутеина;
(b) экспрессию одной или более молекул нуклеиновой кислоты, полученных на стадии (а), в подходящей экспрессионной системе; и
(c) обогащение данного одного или более мутеинами с обнаруживаемой аффинностью связывания для определённой мишени посредством отбора и/или выделения.
2. Мутеин, продуцированный из белка Lcn2 способом по п. 1, включающий, по меньшей мере, один мутированный аминокислотный остаток в любой позиции 100 и/или 106 и по меньшей мере 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17 или 18 мутированных аминокислотных остатков в любой из позиций 36, 40, 41, 49, 52, 68, 70, 72, 73, 77, 79, 81, 96, 103, 125, 127, 132, и 134 линейной полипептидной последовательности Lcn2 и связывающий предварительно выбранную мишень с различимой аффинностью, не связывающуюся с нативным зрелым Lcn2 при физиологических условиях.
3. Мутеин по п. 2, содержащий 18, 19 или 20 мутированных аминокислотных остатков в любом из положений 36, 40, 41, 4 9, 52, 68, 70, 72, 73, 77, 79, 81, 96, 100, 103, 106, 125, 127, 132 и 134 в линейной полипептидной последовательности Lcn2.
4. Мутеин по п. 2, где указанная мишень выбрана из группы, состоящей из пептида, белка, фрагмента или домена белка, и небольшой органической молекулы.
5. Мутеин по п. 4, где пептид является бета-амилоидным пептидом.
6. Мутеин по п. 5, где бета-амилоидный пептид является Ар40 пептидом или пептидом Aβ42.
7. Мутеин по п. 5, содержащий по сравнению со зрелой аминокислотной последовательностью hLcn2 дикого типа, по меньшей мере, 3, 4, 5, 6, 7, 8, 9, 10, 11 или 12 аминокислотных замещений, выбранных из группы, состоящей из Leu36 → Val or Cys; Ala40 → Tyr or Lys or Val; Ile41→Thr or Ser or Leu; GIn49 → Leu or Trp; Leu70 → Gly; Arg72→ Gly or Asp; Lys73 → Leu or Thr or Asp; Asp77 → Asn or His or Leu; Trp79 → Lys; Asn96 → Ile or Arg; Tyr100 → Gln or Arg or Glu; Leu103 → Met or Arg or GIy; Tyr106 → Tyr or Ala or Trp; Lys125 → Thr or Val or Glu; Ser127 → Gly or GIn or Ala; Tyr132 → Met or Ser or Thr; and Lys134 → Asn
8. Мутеин по п. 7, имеющий последовательность SEQ ID N0: 39 (S1-A4) или SEQ ID N0: 41 (US7) или SEQ ID N0: 43 (Hl-Gl).
9. Мутеин по п. 4, где белком является фибронектин или его домен.
10. Мутеин по п. 9, где доменом фибронектина является экстрадомен В или его фрагмент.
11. Мутеин по п. 9, содержащий по сравнению со зрелой аминокислотной последовательностью hLcn2 дикого типа по меньшей мере 3, 4, 5, 6, 7, 8, 9, 10, 11 или 12 аминокислотных замещений, выбранных из группы, состоящей из Leu36 → Lys or Glu or Arg or Ala; Ala40 → His or Met or Thr or Ser; Ile41 → Asp or Arg or Trp or Leu; Gln49 → Arg or Ala or Tyr; Leu70 → Leu or Arg or Met; Arg72→ Val or Arg or Gln or Met; Lys73 → His or Arg or Ser; Asp77→ Asn or His or Lys or Arg; Trp79 → Arg or Met or Leu; Asn96 → Lys or Ala or Ser; Tyr100→Trp or Pro or Lys; Leu103 → His or Pro; Tyr106 → Phe or Trp or Thr; Lys125 → Arg or His or Thr; Ser127 → Tyr or Phe Ala; Tyr132 → Leu or Phe; Lys134 → Glu or His or GIy or Phe; and Ser146 → Asn.
12. Мутеин по п. 11, имеющий последовательность SEQ ID N0: 22 (N7E) или SEQ ID N0: 24 (N9B) или SEQ ID N0: 26 (N10D).
13. Мутеин по любому из п.п. 2 – 12, далее содержащий по сравнению со зрелой аминокислотной последовательностью hLcn2 дикого типа аминокислотные замещения, выбранные из группы, состоящей из GIn28 → His and Cys87 → Ser.
14. Мутеин по любому из пп. 2 – 12, конъюгирующий с соединением, выбранным из группы, состоящей из органических молекул, ферментных меток, радиоактивных меток, цветовых меток, флуоресцентных меток, хромогенных меток, люминесцентных меток, гаптенов, дигоксигенина, биотина, цитостатических агентов, токсинов, металлических комплексов, металлов и коллоидного золота, и с соединением, продлевающим серологический период полураспада мутеина, или сопряжённый своим N-терминалом и/или C-терминалом с элементом слияния, представляющим собой белок или домен белка или пептид.
15. Мутеин по любому из пп. 2 – 12, связывающий данную неприродную мишень с КД 1 µM или менее, или 100 µM или менее, или 1 µM или менее, или 500 нм, или 200 нм или менее, или 100 нм или менее, или 50 нм или менее, или 10 нм или менее, или 1 нм или менее.
16. Молекула нуклеиновой кислоты, содержащая нуклеотидную последовательность, кодирующую мутеин по любому из пп. 2 – 15.
17. Клетка-хозяин для экспрессии мутеина по любому из пп. 2 – 15, содержащая молекулу нуклеиновой кислоты по п. 16.
18. Способ получения мутеина по любому из пп. 2 – 15, включающий культивирование клетки-хозяина по п. 17 для экспрессии мутеина по любому из пп. 2 – 15.
19. Диагностический набор для связывания предварительно выбранной мишени, включающий в качестве одного из компонентов набора мутеин по любому из пп. 2 – 15.
20. Аналитический набор для связывания предварительно выбранной мишени, включающий в качестве одного из компонентов набора мутеин по любому из пп. 2 – 15.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US26709809P | 2009-12-07 | 2009-12-07 | |
| US61/267,098 | 2009-12-07 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2012128586/10A Division RU2564125C2 (ru) | 2009-12-07 | 2010-12-07 | Мутеины липокалина 2 человека (lcn2, hngal) с аффинностью для определенной мишени |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2019132267A Division RU2804336C2 (ru) | 2019-10-11 | МУТЕИНЫ ЛИПОКАЛИНА 2 ЧЕЛОВЕКА (Lcn2, hNGAL) С АФФИННОСТЬЮ ДЛЯ ОПРЕДЕЛЕННОЙ МИШЕНИ |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| RU2015137158A true RU2015137158A (ru) | 2018-12-25 |
| RU2015137158A3 RU2015137158A3 (ru) | 2018-12-25 |
| RU2707126C2 RU2707126C2 (ru) | 2019-11-22 |
Family
ID=43597993
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2012128586/10A RU2564125C2 (ru) | 2009-12-07 | 2010-12-07 | Мутеины липокалина 2 человека (lcn2, hngal) с аффинностью для определенной мишени |
| RU2015137158A RU2707126C2 (ru) | 2009-12-07 | 2010-12-07 | МУТЕИНЫ ЛИПОКАЛИНА 2 ЧЕЛОВЕКА (Lcn2, hNGAL) С АФФИННОСТЬЮ ДЛЯ ОПРЕДЕЛЕННОЙ МИШЕНИ |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2012128586/10A RU2564125C2 (ru) | 2009-12-07 | 2010-12-07 | Мутеины липокалина 2 человека (lcn2, hngal) с аффинностью для определенной мишени |
Country Status (12)
| Country | Link |
|---|---|
| US (3) | US9549968B2 (ru) |
| EP (3) | EP2990798B1 (ru) |
| JP (5) | JP5913120B2 (ru) |
| KR (2) | KR101842076B1 (ru) |
| CN (1) | CN102770764B (ru) |
| AU (1) | AU2010329995B2 (ru) |
| BR (1) | BR112012013662B1 (ru) |
| CA (1) | CA2779562C (ru) |
| DK (2) | DK2990798T3 (ru) |
| RU (2) | RU2564125C2 (ru) |
| SG (1) | SG10201408073XA (ru) |
| WO (1) | WO2011069992A2 (ru) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK2990798T3 (da) | 2009-12-07 | 2019-12-02 | Pieris Pharmaceuticals Gmbh | Muteiner af human lipocalin 2 (lcn2, hngal) med affinitet for et specifikt mål |
| SG10201505785QA (en) * | 2010-08-16 | 2015-09-29 | Pieris Ag | Binding proteins for hepcidin |
| EP2809679A1 (en) | 2012-01-31 | 2014-12-10 | Technische Universität München | Muteins of a1m lipocalin and method of production therefor |
| US9522940B2 (en) | 2012-05-23 | 2016-12-20 | Pieris Pharmaceuticals Gmbh | Lipocalin muteins with binding-affinity for glypican-3 (GPC-3) and use of lipocalin muteins for target-specific delivery to cells expressing GPC-3 |
| EP2920202B1 (en) | 2012-11-19 | 2018-08-29 | Pieris Pharmaceuticals GmbH | Novel specific-binding polypeptides and uses thereof |
| EP3789397B1 (en) | 2014-05-22 | 2023-12-06 | Pieris Pharmaceuticals GmbH | Novel specific-binding polypeptides and uses thereof |
| WO2016120307A1 (en) | 2015-01-28 | 2016-08-04 | Pieris Ag | Novel proteins specific for angiogenesis |
| EP3064507A1 (en) | 2015-03-06 | 2016-09-07 | Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts | Fusion proteins comprising a binding protein and an interleukin-15 polypeptide having a reduced affinity for IL15ra and therapeutic uses thereof |
| KR20170142200A (ko) * | 2015-05-04 | 2017-12-27 | 피어이스 파마슈티컬즈 게엠베하 | Cd137에 특이적인 신규한 단백질 |
| AU2016258977C1 (en) * | 2015-05-04 | 2022-07-14 | Pieris Pharmaceuticals Gmbh | Anti-cancer fusion polypeptide |
| MX2017014730A (es) * | 2015-05-18 | 2018-06-28 | Pieris Pharmaceuticals Gmbh | Muteinas de lipocalina 2 humana con afinidad por glipicano-3 (gpc3). |
| MX2017014716A (es) | 2015-05-18 | 2018-06-28 | Pieris Pharmaceuticals Gmbh | Polipeptido de fusion anti-cancer. |
| EP3115371A1 (en) | 2015-07-07 | 2017-01-11 | Sanofi | Fusion molecules |
| MX2018006559A (es) * | 2015-11-30 | 2019-06-12 | Pieris Australia Pty Ltd | Nuevos polipeptidos de fusion anti-angiogenicos. |
| TW201725212A (zh) | 2015-12-10 | 2017-07-16 | 第一三共股份有限公司 | 特異性於降鈣素基因相關胜肽的新穎蛋白 |
| US20190257832A1 (en) | 2016-07-18 | 2019-08-22 | Philippe Ulsemer | Natural microorganisms which are naturally capable of binding toxins and/or toxin receptors |
| GB201615254D0 (en) | 2016-09-08 | 2016-11-23 | Ge Healthcare Bio Sciences Ab | Novel Ligand and use thereof |
| CN107403074B (zh) * | 2017-06-09 | 2018-05-29 | 天津市湖滨盘古基因科学发展有限公司 | 一种突变蛋白的检测方法及装置 |
| EP3715370A1 (en) * | 2019-03-28 | 2020-09-30 | Technische Universität München | High affinity anticalins directed against human cd98hc |
| AU2020253034A1 (en) | 2019-03-29 | 2021-08-19 | Pieris Pharmaceuticals Gmbh | Inhaled administration of lipocalin muteins |
Family Cites Families (43)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4376110A (en) | 1980-08-04 | 1983-03-08 | Hybritech, Incorporated | Immunometric assays using monoclonal antibodies |
| ATE76311T1 (de) | 1986-08-19 | 1992-06-15 | Genentech Inc | Einrichtung und dispersion zum intrapulmonalen eingeben von polypeptidwuchsstoffen und zytokinen. |
| JPH01215289A (ja) | 1988-02-22 | 1989-08-29 | Toa Nenryo Kogyo Kk | 遺伝子組換えによる正常ヒト血清アルブミンａの製造方法 |
| FR2649991B2 (fr) | 1988-08-05 | 1994-03-04 | Rhone Poulenc Sante | Utilisation de derives stables du plasmide pkd1 pour l'expression et la secretion de proteines heterologues dans les levures du genre kluyveromyces |
| US5728553A (en) | 1992-09-23 | 1998-03-17 | Delta Biotechnology Limited | High purity albumin and method of producing |
| KR100384353B1 (ko) | 1994-05-18 | 2003-10-04 | 네크타르 테라퓨틱스 | 인터페론의건조분말제형을제조하기위한방법및조성물 |
| DE4417598A1 (de) | 1994-05-19 | 1995-12-14 | Max Planck Gesellschaft | Verwendung des Tetracyclinpromotors zur stringent regulierten Produktion von rekombinanten Proteinen in prokaryontischen Zellen |
| WO1996023879A1 (en) | 1995-01-30 | 1996-08-08 | Terrapin Technologies, Inc. | Glubodies - multiplicities of proteins capable of binding a variety of small molecules |
| US5908621A (en) | 1995-11-02 | 1999-06-01 | Schering Corporation | Polyethylene glycol modified interferon therapy |
| US6620413B1 (en) | 1995-12-27 | 2003-09-16 | Genentech, Inc. | OB protein-polymer chimeras |
| DE19641876B4 (de) | 1996-10-10 | 2011-09-29 | Iba Gmbh | Streptavidinmuteine |
| WO1998016873A1 (fr) | 1996-10-14 | 1998-04-23 | Firm Forsat Ltd. | Procede de preparation de dispersions a base de composants chromogenes |
| EP1009762A2 (en) | 1997-08-06 | 2000-06-21 | ZymoGenetics, Inc. | Lipocalin homologs |
| US20140080177A1 (en) | 1997-09-26 | 2014-03-20 | Pieris Ag | Anticalins |
| DE19742706B4 (de) * | 1997-09-26 | 2013-07-25 | Pieris Proteolab Ag | Lipocalinmuteine |
| GB9722131D0 (en) | 1997-10-20 | 1997-12-17 | Medical Res Council | Method |
| CA2233725A1 (en) | 1998-03-31 | 1999-09-30 | Hemosol Inc. | Hemoglobin-hydroxyethyl starch complexes |
| IL139786A0 (en) | 1998-06-08 | 2002-02-10 | Hoffmann La Roche | Use of peg-ifn-alpha and ribavirin for the treatment of chronic hepatitis c |
| US6403564B1 (en) | 1998-10-16 | 2002-06-11 | Schering Corporation | Ribavirin-interferon alfa combination therapy for eradicating detectable HCV-RNA in patients having chronic hepatitis C infection |
| US6566073B1 (en) | 1998-10-19 | 2003-05-20 | Ariad Gene Therapeutics, Inc. | Materials and methods involving conditional retention domains |
| CN1279053C (zh) * | 1999-03-04 | 2006-10-11 | 普雷西斯药品公司 | 含有D－氨基酸的β－淀粉样肽聚集的调节因子 |
| DE19926068C1 (de) | 1999-06-08 | 2001-01-11 | Arne Skerra | Muteine des Bilin-Bindungsproteins |
| US7211395B2 (en) | 2001-03-09 | 2007-05-01 | Dyax Corp. | Serum albumin binding moieties |
| CA2464690A1 (en) | 2001-09-27 | 2003-04-10 | Pieris Proteolab Ag | Muteins of apolipoprotein d |
| WO2003029462A1 (en) * | 2001-09-27 | 2003-04-10 | Pieris Proteolab Ag | Muteins of human neutrophil gelatinase-associated lipocalin and related proteins |
| EP1658308A1 (en) | 2003-08-25 | 2006-05-24 | Pieris ProteoLab AG | Muteins of a bilin-binding protein with affinity for a given target |
| AU2003275958A1 (en) * | 2003-08-25 | 2005-03-10 | Pieris Proteolab Ag | Muteins of tear lipocalin |
| JP2007284351A (ja) | 2004-07-27 | 2007-11-01 | Osaka Bioscience Institute | アミロイド蛋白質の凝集を抑制する物質とその作用 |
| WO2006056464A2 (en) | 2004-11-26 | 2006-06-01 | Pieris Ag | Compound with affinity for the cytotoxic t lymphocyte-associated antigen (ctla-4) |
| AU2006294644A1 (en) | 2005-09-27 | 2007-04-05 | Amunix, Inc. | Proteinaceous pharmaceuticals and uses thereof |
| ES2354653T3 (es) * | 2006-08-01 | 2011-03-16 | Pieris Ag | Muteínas de lipocalina lacrimal y procedimientos para obtener las mismas. |
| CN105112477A (zh) * | 2006-08-01 | 2015-12-02 | 皮里斯股份公司 | 泪液脂质运载蛋白的突变蛋白及其获得方法 |
| JP5848006B2 (ja) * | 2007-10-19 | 2016-01-27 | アボット・ラボラトリーズＡｂｂｏｔｔ Ｌａｂｏｒａｔｏｒｉｅｓ | Ｎｇａｌの検出用イムノアッセイ及びキット |
| US8420051B2 (en) | 2008-06-24 | 2013-04-16 | Technische Universitaet Meunchen | Muteins of hNGAL and related proteins with affinity for a given target |
| DK2990798T3 (da) | 2009-12-07 | 2019-12-02 | Pieris Pharmaceuticals Gmbh | Muteiner af human lipocalin 2 (lcn2, hngal) med affinitet for et specifikt mål |
| SG10201505785QA (en) * | 2010-08-16 | 2015-09-29 | Pieris Ag | Binding proteins for hepcidin |
| US9260492B2 (en) | 2010-11-15 | 2016-02-16 | Pieris Ag | Muteins of human lipocalin 2 with affinity for glypican-3 (GPC-3) |
| EP2920202B1 (en) * | 2012-11-19 | 2018-08-29 | Pieris Pharmaceuticals GmbH | Novel specific-binding polypeptides and uses thereof |
| WO2016120307A1 (en) | 2015-01-28 | 2016-08-04 | Pieris Ag | Novel proteins specific for angiogenesis |
| WO2016131804A1 (en) | 2015-02-18 | 2016-08-25 | Sanofi | Novel proteins specific for pyoverdine and pyochelin |
| KR20170142200A (ko) | 2015-05-04 | 2017-12-27 | 피어이스 파마슈티컬즈 게엠베하 | Cd137에 특이적인 신규한 단백질 |
| MX2017014730A (es) | 2015-05-18 | 2018-06-28 | Pieris Pharmaceuticals Gmbh | Muteinas de lipocalina 2 humana con afinidad por glipicano-3 (gpc3). |
| TW201725212A (zh) | 2015-12-10 | 2017-07-16 | 第一三共股份有限公司 | 特異性於降鈣素基因相關胜肽的新穎蛋白 |
-
2010
- 2010-12-07 DK DK15186647T patent/DK2990798T3/da active
- 2010-12-07 EP EP15186647.2A patent/EP2990798B1/en active Active
- 2010-12-07 CN CN201080055370.5A patent/CN102770764B/zh active Active
- 2010-12-07 US US13/514,133 patent/US9549968B2/en active Active
- 2010-12-07 RU RU2012128586/10A patent/RU2564125C2/ru active
- 2010-12-07 AU AU2010329995A patent/AU2010329995B2/en active Active
- 2010-12-07 JP JP2012542505A patent/JP5913120B2/ja active Active
- 2010-12-07 WO PCT/EP2010/069028 patent/WO2011069992A2/en active Application Filing
- 2010-12-07 EP EP10784824.4A patent/EP2510357B1/en active Active
- 2010-12-07 DK DK10784824.4T patent/DK2510357T3/en active
- 2010-12-07 KR KR1020127017730A patent/KR101842076B1/ko active IP Right Grant
- 2010-12-07 RU RU2015137158A patent/RU2707126C2/ru active
- 2010-12-07 SG SG10201408073XA patent/SG10201408073XA/en unknown
- 2010-12-07 BR BR112012013662-3A patent/BR112012013662B1/pt active IP Right Grant
- 2010-12-07 EP EP19198835.1A patent/EP3660510A3/en active Pending
- 2010-12-07 CA CA2779562A patent/CA2779562C/en active Active
- 2010-12-07 KR KR1020187007781A patent/KR101974044B1/ko active IP Right Grant
-
2015
- 2015-12-02 JP JP2015236010A patent/JP6346159B2/ja active Active
-
2016
- 2016-12-15 US US15/380,168 patent/US10618941B2/en active Active
-
2018
- 2018-05-24 JP JP2018099240A patent/JP2018148914A/ja active Pending
-
2020
- 2020-03-06 US US16/812,069 patent/US11827681B2/en active Active
- 2020-07-07 JP JP2020117175A patent/JP2021006024A/ja active Pending
-
2022
- 2022-07-01 JP JP2022106985A patent/JP7482544B2/ja active Active
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| RU2015137158A (ru) | МУТЕИНЫ ЛИПОКАЛИНА 2 ЧЕЛОВЕКА (Lcn2,hNGAL) С АФФИННОСТЬЮ ДЛЯ ОПРЕДЕЛЕННОЙ МИШЕНИ | |
| JP2013512683A5 (ru) | ||
| JP7118510B2 (ja) | ｐｏｌＸファミリーＤＮＡポリメラーゼのバリアント | |
| Iyengar et al. | The complete amino‐acid sequence of the sweet protein thaumatin I | |
| JP2014502149A5 (ru) | ||
| JP2018519802A5 (ru) | ||
| JP2021006024A5 (ru) | ||
| RU2016149596A (ru) | Новые полипептиды со специфическим связыванием и пути их применения | |
| Mullerpatan et al. | Purification of proteins using peptide-ELP based affinity precipitation | |
| RU2017135599A (ru) | Белки, специфичные в отношении cd137 | |
| JP2018515084A5 (ru) | ||
| RU2017135605A (ru) | Мутеины липокалина 2 человека с аффинностью в отношении глипикана-3 (gpc3) | |
| JP2019506140A5 (ru) | ||
| RU2015142437A (ru) | Новые связывающие белки для pcsk9 | |
| JP2020505948A5 (ru) | ||
| WO2009122442A2 (en) | Mutants of streptokinase and their covalently modified forms | |
| WO2022115715A1 (en) | Compositions and methods for selective depletion of target molecules | |
| CA2434737A1 (en) | Non aggregating fluorescent proteins and methods for using the same | |
| RU2010102859A (ru) | Улучшенные способы образования дисульфидных связей | |
| Lowey et al. | Neonatal and adult myosin heavy chains form homodimers during avian skeletal muscle development. | |
| JP2019163270A (ja) | 親和性タンパク質及びその使用 | |
| Jones et al. | Complete amino acid sequence of the myoglobin from the Atlantic bottlenosed dolphin, Tursiops truncatus | |
| KR102140557B1 (ko) | 단백질-단백질 결합체를 형성 매개 펩타이드 및 이를 이용한 단백질-단백질 결합체 형성 방법 | |
| Pliszka et al. | Interaction of the N-terminal part of the A1 essential light chain with the myosin heavy chain | |
| Käkönen et al. | Purification and characterization of recombinant osteocalcin fusion protein expressed inEscherichia coli |