RU2015117547A - Способ и системы для оптимизации системы перфузионной культуры клеток - Google Patents
Способ и системы для оптимизации системы перфузионной культуры клеток Download PDFInfo
- Publication number
- RU2015117547A RU2015117547A RU2015117547A RU2015117547A RU2015117547A RU 2015117547 A RU2015117547 A RU 2015117547A RU 2015117547 A RU2015117547 A RU 2015117547A RU 2015117547 A RU2015117547 A RU 2015117547A RU 2015117547 A RU2015117547 A RU 2015117547A
- Authority
- RU
- Russia
- Prior art keywords
- cells
- bioreactor
- initial
- perfusion
- initial volume
- Prior art date
Links
- 230000010412 perfusion Effects 0.000 title claims abstract 45
- 238000000034 method Methods 0.000 title claims 31
- 238000005457 optimization Methods 0.000 title 1
- 230000014759 maintenance of location Effects 0.000 claims abstract 21
- 239000012531 culture fluid Substances 0.000 claims abstract 17
- 230000007423 decrease Effects 0.000 claims abstract 10
- 210000004027 cell Anatomy 0.000 claims 64
- 210000004962 mammalian cell Anatomy 0.000 claims 16
- 239000004480 active ingredient Substances 0.000 claims 4
- 210000004978 chinese hamster ovary cell Anatomy 0.000 claims 4
- 101000911390 Homo sapiens Coagulation factor VIII Proteins 0.000 claims 3
- 102000057593 human F8 Human genes 0.000 claims 3
- 229960000900 human factor viii Drugs 0.000 claims 3
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 claims 2
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 claims 2
- 239000003381 stabilizer Substances 0.000 claims 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical group [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims 1
- 102000001690 Factor VIII Human genes 0.000 claims 1
- 108010054218 Factor VIII Proteins 0.000 claims 1
- 229910052791 calcium Inorganic materials 0.000 claims 1
- 239000011575 calcium Substances 0.000 claims 1
- 229960000301 factor viii Drugs 0.000 claims 1
- 230000017854 proteolysis Effects 0.000 claims 1
- 238000004064 recycling Methods 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/745—Blood coagulation or fibrinolysis factors
- C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/14—Bioreactors or fermenters specially adapted for specific uses for producing enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/02—Percolation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/10—Perfusion
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/10—Separation or concentration of fermentation products
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Hematology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261712190P | 2012-10-10 | 2012-10-10 | |
| US61/712,190 | 2012-10-10 | ||
| PCT/US2013/064159 WO2014059035A1 (en) | 2012-10-10 | 2013-10-09 | Methods and systems for optimizing perfusion cell culture system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| RU2015117547A true RU2015117547A (ru) | 2016-12-10 |
Family
ID=49448331
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2015117547A RU2015117547A (ru) | 2012-10-10 | 2013-10-09 | Способ и системы для оптимизации системы перфузионной культуры клеток |
Country Status (15)
| Country | Link |
|---|---|
| US (2) | US20150299638A1 (https=) |
| EP (1) | EP2906677A1 (https=) |
| JP (1) | JP6393267B2 (https=) |
| KR (1) | KR20150063541A (https=) |
| CN (1) | CN104822821A (https=) |
| AR (1) | AR092967A1 (https=) |
| AU (1) | AU2013329318A1 (https=) |
| CA (1) | CA2887581A1 (https=) |
| HK (1) | HK1213285A1 (https=) |
| IL (1) | IL238179A0 (https=) |
| MX (1) | MX2015004516A (https=) |
| RU (1) | RU2015117547A (https=) |
| SG (2) | SG11201502741WA (https=) |
| TW (1) | TW201418455A (https=) |
| WO (1) | WO2014059035A1 (https=) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9944894B2 (en) | 2015-01-16 | 2018-04-17 | General Electric Company | Pluripotent stem cell expansion and passage using a rocking platform bioreactor |
| CN105385731B (zh) * | 2015-12-25 | 2018-10-30 | 上海莱士血液制品股份有限公司 | 一种表达重组八因子的灌注培养方法 |
| ITUB20160272A1 (it) * | 2016-01-22 | 2017-07-22 | Univ Degli Studi Di Palermo | Bioreattore a perfusione autosufficiente monouso per crescite cellulari 3D |
| JP6943449B2 (ja) * | 2016-03-21 | 2021-09-29 | グローバル・ライフ・サイエンシズ・ソリューションズ・ユーエスエー・エルエルシー | 撹拌タンクバイオリアクタを用いた多能性幹細胞の増殖および継代 |
| WO2019071076A1 (en) | 2017-10-06 | 2019-04-11 | Lonza Ltd. | AUTOMATED CONTROL OF CELL CULTURE BY SPECTROSCOPY RAMAN |
| IL315801A (en) | 2017-10-16 | 2024-11-01 | Regeneron Pharma | Perfusion bioreactor and methods of using it |
| WO2019226618A1 (en) * | 2018-05-22 | 2019-11-28 | Nantkwest, Inc. | Methods and systems for cell bed formation during bioprocessing |
| US20210214702A1 (en) * | 2018-06-01 | 2021-07-15 | Gennova Biopharmaceuticals Limited | Process for production of recombinant tnk-tpa by packed-bed perfusion system |
| EP3875595A4 (en) * | 2018-11-02 | 2022-07-13 | Kyowa Kirin Co., Ltd. | Liquid medium preparation method |
| GB201903813D0 (en) * | 2019-03-20 | 2019-05-01 | Cn Bio Innovations Ltd | Dual circulation microphysiological system |
| WO2020232183A1 (en) * | 2019-05-15 | 2020-11-19 | Life Technologies Corporation | Cell settler apparatus systems and methods for perfusion processes |
| CN114599775B (zh) * | 2019-11-07 | 2025-07-22 | 默克专利股份公司 | 用于进行灌注细胞培养的方法和系统 |
| US12529024B2 (en) | 2019-11-15 | 2026-01-20 | Lonza Ltd | Process and system for producing an inoculum |
| WO2021110870A1 (en) | 2019-12-05 | 2021-06-10 | Acib Gmbh | Method for producing a fermentation product |
| KR20220120606A (ko) * | 2019-12-19 | 2022-08-30 | 우니베르지태트 퓌르 보덴쿨투르 빈 | 고체로부터 공정 재료의 연속 재구성 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6338964B1 (en) | 1999-05-07 | 2002-01-15 | Bayer Corporation | Process and medium for mammalian cell culture under low dissolved carbon dioxide concentration |
| US20040185534A1 (en) * | 2000-10-02 | 2004-09-23 | Knudsen Ida Molgaard | Industrial-scale serum-free production of recombinant proteins in mammalian cells |
| JP2007510416A (ja) * | 2003-11-03 | 2007-04-26 | セントカー・インコーポレーテツド | バイオプロセッシングシステムにおいて低剪断を維持する方法 |
| WO2007071072A1 (en) * | 2005-12-22 | 2007-06-28 | Corporation De L'ecole Polytechnique De Montreal | High-rate perfusion bioreactor |
| WO2007124106A2 (en) * | 2006-04-21 | 2007-11-01 | Bayer Healthcare Llc | Application of antl-apoptotic genes in mammalian cells for perfusion culture. |
| WO2009023562A2 (en) * | 2007-08-09 | 2009-02-19 | Wyeth | Use of perfusion to enhance production of fed-batch cell culture in bioreactors |
| UA106047C2 (uk) * | 2008-06-13 | 2014-07-25 | Сентокор Орто Байотек Інк. | Спосіб отримання високої щільності життєздатних клітин в культурі клітин ссавців |
| CN103415610B (zh) * | 2011-03-18 | 2016-10-19 | 通用电气健康护理生物科学股份公司 | 用于培养细胞的弹性袋 |
-
2013
- 2013-10-09 WO PCT/US2013/064159 patent/WO2014059035A1/en not_active Ceased
- 2013-10-09 RU RU2015117547A patent/RU2015117547A/ru not_active Application Discontinuation
- 2013-10-09 KR KR1020157011445A patent/KR20150063541A/ko not_active Withdrawn
- 2013-10-09 CN CN201380064458.7A patent/CN104822821A/zh active Pending
- 2013-10-09 CA CA2887581A patent/CA2887581A1/en not_active Abandoned
- 2013-10-09 HK HK16101154.2A patent/HK1213285A1/zh unknown
- 2013-10-09 SG SG11201502741WA patent/SG11201502741WA/en unknown
- 2013-10-09 US US14/434,913 patent/US20150299638A1/en not_active Abandoned
- 2013-10-09 AU AU2013329318A patent/AU2013329318A1/en not_active Abandoned
- 2013-10-09 TW TW102136546A patent/TW201418455A/zh unknown
- 2013-10-09 JP JP2015536869A patent/JP6393267B2/ja not_active Expired - Fee Related
- 2013-10-09 US US14/049,676 patent/US20140099711A1/en not_active Abandoned
- 2013-10-09 SG SG10201705806YA patent/SG10201705806YA/en unknown
- 2013-10-09 EP EP13780035.5A patent/EP2906677A1/en not_active Withdrawn
- 2013-10-09 MX MX2015004516A patent/MX2015004516A/es unknown
- 2013-10-10 AR ARP130103674A patent/AR092967A1/es unknown
-
2015
- 2015-04-12 IL IL238179A patent/IL238179A0/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| HK1213285A1 (zh) | 2016-06-30 |
| SG10201705806YA (en) | 2017-08-30 |
| AU2013329318A1 (en) | 2015-05-14 |
| WO2014059035A1 (en) | 2014-04-17 |
| SG11201502741WA (en) | 2015-05-28 |
| EP2906677A1 (en) | 2015-08-19 |
| KR20150063541A (ko) | 2015-06-09 |
| AR092967A1 (es) | 2015-05-06 |
| US20140099711A1 (en) | 2014-04-10 |
| CN104822821A (zh) | 2015-08-05 |
| JP6393267B2 (ja) | 2018-09-19 |
| US20150299638A1 (en) | 2015-10-22 |
| CA2887581A1 (en) | 2014-04-17 |
| IL238179A0 (en) | 2015-05-31 |
| TW201418455A (zh) | 2014-05-16 |
| MX2015004516A (es) | 2015-10-14 |
| JP2015531241A (ja) | 2015-11-02 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FA92 | Acknowledgement of application withdrawn (lack of supplementary materials submitted) |
Effective date: 20180316 |