PL3290528T3 - Sposoby i kompozycje do sekwencjonowania kwasów nukleinowych - Google Patents

Sposoby i kompozycje do sekwencjonowania kwasów nukleinowych

Info

Publication number
PL3290528T3
PL3290528T3 PL17180583T PL17180583T PL3290528T3 PL 3290528 T3 PL3290528 T3 PL 3290528T3 PL 17180583 T PL17180583 T PL 17180583T PL 17180583 T PL17180583 T PL 17180583T PL 3290528 T3 PL3290528 T3 PL 3290528T3
Authority
PL
Poland
Prior art keywords
compositions
methods
nucleic acid
acid sequencing
sequencing
Prior art date
Application number
PL17180583T
Other languages
English (en)
Inventor
Jonathan Mark Boutell
Helmy A. Eltoukhy
Wenyi Feng
Bernard Hirschbein
Thomas Joseph
Robert C. Kain
Kay KLAUSING
Xiaohai Liu
Min-Jui Richard Shen
Geoffrey Paul Smith
Randall Smith
Carolyn TREGIDGO
Xiaolin Wu
Original Assignee
Illumina, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=46964103&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=PL3290528(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Illumina, Inc. filed Critical Illumina, Inc.
Publication of PL3290528T3 publication Critical patent/PL3290528T3/pl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
PL17180583T 2011-09-23 2012-09-21 Sposoby i kompozycje do sekwencjonowania kwasów nukleinowych PL3290528T3 (pl)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201161538294P 2011-09-23 2011-09-23
US201261619878P 2012-04-03 2012-04-03
EP17180583.1A EP3290528B1 (en) 2011-09-23 2012-09-21 Methods and compositions for nucleic acid sequencing
PCT/US2012/056556 WO2013044018A1 (en) 2011-09-23 2012-09-21 Methods and compositions for nucleic acid sequencing
EP12766838.2A EP2768972B2 (en) 2011-09-23 2012-09-21 Methods and compositions for nucleic acid sequencing

Publications (1)

Publication Number Publication Date
PL3290528T3 true PL3290528T3 (pl) 2020-03-31

Family

ID=46964103

Family Applications (2)

Application Number Title Priority Date Filing Date
PL19189927T PL3623481T3 (pl) 2011-09-23 2012-09-21 Kompozycje do sekwencjonowania kwasów nukleinowych
PL17180583T PL3290528T3 (pl) 2011-09-23 2012-09-21 Sposoby i kompozycje do sekwencjonowania kwasów nukleinowych

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PL19189927T PL3623481T3 (pl) 2011-09-23 2012-09-21 Kompozycje do sekwencjonowania kwasów nukleinowych

Country Status (15)

Country Link
US (5) US9453258B2 (pl)
EP (4) EP3290528B1 (pl)
CA (2) CA3104322C (pl)
CY (1) CY1125261T1 (pl)
DK (3) DK2768972T3 (pl)
ES (3) ES2895184T3 (pl)
HR (1) HRP20211523T1 (pl)
HU (1) HUE056246T2 (pl)
LT (1) LT3623481T (pl)
PL (2) PL3623481T3 (pl)
PT (2) PT3623481T (pl)
RS (1) RS62530B1 (pl)
SI (1) SI3623481T1 (pl)
SM (1) SMT202100624T1 (pl)
WO (1) WO2013044018A1 (pl)

Families Citing this family (351)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US11008359B2 (en) 2002-08-23 2021-05-18 Illumina Cambridge Limited Labelled nucleotides
EP2021415B1 (en) 2006-05-18 2017-03-15 Illumina Cambridge Limited Dye compounds and the use of their labelled conjugates
US11940413B2 (en) 2007-02-05 2024-03-26 IsoPlexis Corporation Methods and devices for sequencing nucleic acids in smaller batches
US9163053B2 (en) * 2007-05-18 2015-10-20 Fluidigm Corporation Nucleotide analogs
US9017973B2 (en) 2008-03-19 2015-04-28 Intelligent Biosystems, Inc. Methods and compositions for incorporating nucleotides
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
HRP20151294T1 (hr) 2010-04-05 2016-03-11 Prognosys Biosciences, Inc. Biološka testiranja sa prostornim kodiranjem
US9096899B2 (en) 2010-10-27 2015-08-04 Illumina, Inc. Microdevices and biosensor cartridges for biological or chemical analysis and systems and methods for the same
US9387476B2 (en) 2010-10-27 2016-07-12 Illumina, Inc. Flow cells for biological or chemical analysis
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
EP2697397B1 (en) 2011-04-15 2017-04-05 The Johns Hopkins University Safe sequencing system
DK2768972T3 (en) * 2011-09-23 2017-09-25 Illumina Inc Methods and Compositions for Nucleic Acid Sequencing
EP3590948A1 (en) * 2012-02-15 2020-01-08 Pacific Biosciences of California, Inc. Polymerase enzyme substrates with protein shield
EP2909337B1 (en) 2012-10-17 2019-01-09 Spatial Transcriptomics AB Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
WO2014070462A1 (en) 2012-10-29 2014-05-08 The Johns Hopkins University Papanicolaou test for ovarian and endometrial cancers
US9593373B2 (en) 2013-03-15 2017-03-14 Illumina Cambridge Limited Modified nucleosides or nucleotides
WO2014210223A1 (en) 2013-06-25 2014-12-31 Prognosys Biosciences, Inc. Spatially encoded biological assays using a microfluidic device
US9193999B2 (en) 2013-07-03 2015-11-24 Illumina, Inc. Sequencing by orthogonal synthesis
CN113789372B (zh) 2013-08-08 2025-02-25 伊鲁米那股份有限公司 用于向流动池的试剂输送的流体系统
SMT202000434T1 (it) * 2013-12-03 2020-09-10 Illumina Inc Metodi e sistemi per l'analisi di dati di immagine
PL3108009T3 (pl) 2014-02-18 2021-10-11 Illumina, Inc. Sposoby i kompozycje do profilowania dna
CN106459869B (zh) 2014-03-11 2020-01-21 伊鲁米那股份有限公司 一次性的集成微流体盒及其制备和使用的方法
EP3143161B1 (en) * 2014-05-16 2021-04-21 Illumina, Inc. Nucleic acid synthesis techniques
JP6960223B2 (ja) 2014-05-27 2021-11-05 イラミーナ インコーポレーテッド 基礎器具と取外可能カートリッジとを含む生化学的解析のためのシステム及び方法
US20150353989A1 (en) 2014-06-09 2015-12-10 Illumina Cambridge Limited Sample preparation for nucleic acid amplification
GB201414098D0 (en) * 2014-08-08 2014-09-24 Illumina Cambridge Ltd Modified nucleotide linkers
WO2016040602A1 (en) 2014-09-11 2016-03-17 Epicentre Technologies Corporation Reduced representation bisulfite sequencing using uracil n-glycosylase (ung) and endonuclease iv
WO2016040607A1 (en) 2014-09-12 2016-03-17 Illumina, Inc. Compositions, systems, and methods for detecting the presence of polymer subunits using chemiluminescence
US9897791B2 (en) 2014-10-16 2018-02-20 Illumina, Inc. Optical scanning systems for in situ genetic analysis
GB201418718D0 (en) * 2014-10-21 2014-12-03 Cambridge Epigenetix Ltd Improved nucleic acid re-sequencing using a reduced number of identified bases
WO2016065248A1 (en) 2014-10-23 2016-04-28 Complete Genomics, Inc. Signal confinement sequencing (scs) and nucleotide analogues for signal confinement sequencing
GB201419731D0 (en) 2014-11-05 2014-12-17 Illumina Cambridge Ltd Sequencing from multiple primers to increase data rate and density
US9828627B2 (en) 2014-11-05 2017-11-28 Illumina Cambridge Limited Reducing DNA damage during sample preparation and sequencing using siderophore chelators
EP3218511B1 (en) 2014-11-11 2020-04-22 Illumina Cambridge Limited Methods and arrays for producing and sequencing monoclonal clusters of nucleic acid
EP3227684B1 (en) 2014-12-03 2019-10-02 Isoplexis Corporation Analysis and screening of cell secretion profiles
WO2016154038A1 (en) 2015-03-20 2016-09-29 Illumina, Inc. Fluidics cartridge for use in the vertical or substantially vertical position
EP3277839B1 (en) 2015-03-31 2020-12-02 Illumina Cambridge Limited Surface concatamerization of templates
ES2955488T3 (es) 2015-04-10 2023-12-01 Spatial Transcriptomics Ab Análisis multiplex de especímenes biológicos de ácidos nucleicos espacialmente distinguidos
JP6692831B2 (ja) 2015-05-12 2020-05-13 イラミーナ インコーポレーテッド 核酸をシーケンシングするための電界効果装置および方法
AU2016269785B2 (en) 2015-05-29 2019-02-28 Illumina Cambridge Limited Enhanced utilization of surface primers in clusters
US10640809B2 (en) 2015-05-29 2020-05-05 Epicentre Technologies Corporation Methods of analyzing nucleic acids
DK3320111T3 (da) 2015-07-06 2021-07-19 Illumina Cambridge Ltd Prøveklargøring til nukleinsyreamplifikation
CA3242290A1 (en) 2015-07-27 2017-02-02 Illumina, Inc. Spatial mapping of nucleic acid sequence information
IL305561A (en) 2015-07-30 2023-10-01 Illumina Inc Removal of orthogonal blocking of nucleotides
US11286531B2 (en) 2015-08-11 2022-03-29 The Johns Hopkins University Assaying ovarian cyst fluid
CN105112516A (zh) * 2015-08-14 2015-12-02 深圳市瀚海基因生物科技有限公司 一种单分子靶向测序方法、装置、系统及应用
DK3334839T3 (da) 2015-08-14 2021-04-26 Illumina Inc Systemer og fremgangsmåder under anvendelse af magnetisk reagerende sensorer til bestemmelse af en genetisk egenskab
CN116338219A (zh) 2015-08-24 2023-06-27 亿明达股份有限公司 用于生物和化学测定的线路内蓄压器和流量控制系统
CN114989235A (zh) 2015-09-28 2022-09-02 哥伦比亚大学董事会 用作dna合成测序的可逆终止物的基于新的二硫键接头的核苷酸的设计与合成
WO2017087879A2 (en) * 2015-11-18 2017-05-26 Synergy Pharmaceuticals, Inc. Compositions and method for the treatment and detection of colon cancer
EP3433604B1 (en) 2016-03-24 2022-03-30 Illumina, Inc. Photonic superlattice-based devices for use in luminescent imaging, and methods of using the same
CN109690359B (zh) 2016-04-22 2021-10-15 伊鲁米那股份有限公司 在像素内的多个位点的发光成像中使用的基于光子结构的设备和组成物及使用其的方法
WO2017185026A1 (en) * 2016-04-22 2017-10-26 Complete Genomics, Inc. Reversibly blocked nucleoside analogues and their use
CN109661232B (zh) 2016-05-23 2023-03-03 纽约哥伦比亚大学董事会 核苷酸衍生物及其使用方法
JP7155021B2 (ja) 2016-07-22 2022-10-18 オレゴン ヘルス アンド サイエンス ユニヴァーシティ 単細胞全ゲノムライブラリおよびそれを作成する組み合わせインデックス付加方法
CN109844136A (zh) 2016-08-15 2019-06-04 欧姆尼奥姆股份有限公司 测序核酸的方法和系统
CN109964126B (zh) 2016-09-12 2022-12-27 伊索普莱克西斯公司 用于细胞治疗法和其他免疫治疗法的多重分析的系统和方法
US10385214B2 (en) 2016-09-30 2019-08-20 Illumina Cambridge Limited Fluorescent dyes and their uses as biomarkers
EP3538891B1 (en) 2016-11-11 2022-01-05 Isoplexis Corporation Compositions and methods for the simultaneous genomic, transcriptomic and proteomic analysis of single cells
US11525783B2 (en) 2016-11-22 2022-12-13 IsoPlexis Corporation Systems, devices and methods for cell capture and methods of manufacture thereof
WO2018114710A1 (en) 2016-12-22 2018-06-28 Illumina Cambridge Limited Coumarin compounds and their uses as fluorescent labels
EP3564387A4 (en) * 2016-12-27 2020-10-07 EGI Tech (Shen Zhen) Co., Limited SINGLE FLUORESCENT DYE BASED SEQUENCING METHOD
FI4112741T3 (fi) * 2017-01-04 2025-05-15 Mgi Tech Co Ltd Vaiheittainen sekvensointi ei-leimatuilla, reversiibeleillä terminaattoreilla tai luonnollisilla nukleotideilla
CA3049142A1 (en) 2017-01-06 2018-07-12 Illumina, Inc. Phasing correction
KR102326612B1 (ko) 2017-01-17 2021-11-15 일루미나, 인코포레이티드 종양원성 스플라이스 변이체 결정
GB201701688D0 (en) 2017-02-01 2017-03-15 Illumia Inc System and method with fiducials in non-recliner layouts
GB201701689D0 (en) 2017-02-01 2017-03-15 Illumia Inc System and method with fiducials of non-closed shapes
GB201701686D0 (en) 2017-02-01 2017-03-15 Illunina Inc System & method with fiducials having offset layouts
US11896944B2 (en) 2017-02-01 2024-02-13 Illumina, Inc. System and method with fiducials responding to multiple excitation frequencies
US12018325B2 (en) 2017-03-28 2024-06-25 The Trustees Of Columbia University In The City Of New York 3′-O-modified nucleotide analogues with different cleavable linkers for attaching fluorescent labels to the base for DNA sequencing by synthesis
EP3913053A1 (en) 2017-04-23 2021-11-24 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
US9951385B1 (en) * 2017-04-25 2018-04-24 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
US10161003B2 (en) 2017-04-25 2018-12-25 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
SG10202108175RA (en) 2017-06-07 2021-09-29 Univ Oregon Health & Science Single cell whole genome libraries for methylation sequencing
US11542540B2 (en) * 2017-06-16 2023-01-03 Life Technologies Corporation Control nucleic acids, and compositions, kits, and uses thereof
WO2018236631A1 (en) 2017-06-20 2018-12-27 Illumina, Inc. METHODS AND COMPOSITIONS FOR RESOLVING INEFFICIENCY IN AMPLIFICATION REACTIONS
CN109207571B (zh) * 2017-06-30 2022-01-04 深圳华大生命科学研究院 一种检测核酸内切酶酶切位点的方法
US20200202977A1 (en) 2017-07-31 2020-06-25 Illumina, Inc. Sequencing system with multiplexed biological sample aggregation
DK3662083T3 (da) 2017-08-01 2024-11-18 Illumina Inc Rumlig indeksering af genetisk materiale og biblioteksfremstilling under anvendelse af hydrogelperler og flowceller
IL319255A (en) 2017-08-07 2025-04-01 Univ Johns Hopkins Methods and materials for cancer assessment and treatment
CA3067421C (en) 2017-11-06 2023-08-15 Illumina, Inc. Nucleic acid indexing techniques
NL2020636B1 (en) * 2017-12-28 2019-07-08 Illumina Inc Light energy fluorescence excitation
NZ759656A (en) 2018-01-08 2022-07-01 Illumina Inc Systems and devices for high-throughput sequencing with semiconductor-based detection
WO2019136388A1 (en) 2018-01-08 2019-07-11 Illumina, Inc. Systems and devices for high-throughput sequencing with semiconductor-based detection
WO2019161253A1 (en) 2018-02-16 2019-08-22 Ultima Genomics, Inc. Methods for sequencing with single frequency detection
CN112218640A (zh) 2018-03-15 2021-01-12 哥伦比亚大学董事会 核苷酸类似物及其在核酸测序和分析中的用途
AU2019248635B2 (en) 2018-04-02 2022-01-27 Illumina, Inc. Compositions and methods for making controls for sequence-based genetic testing
AU2019255987A1 (en) 2018-04-19 2020-12-10 Pacific Biosciences Of California, Inc. Improving accuracy of base calls in nucleic acid sequencing methods
AU2019270185A1 (en) 2018-05-17 2020-01-16 Illumina, Inc. High-throughput single-cell sequencing with reduced amplification bias
US11180794B2 (en) 2018-05-31 2021-11-23 Omniome, Inc. Methods and compositions for capping nucleic acids
JP2021525078A (ja) 2018-05-31 2021-09-24 オムニオム インコーポレイテッドＯｍｎｉｏｍｅ， Ｉｎｃ． 核酸配列決定におけるシグナル対ノイズの増加
JP6986103B2 (ja) 2018-06-04 2021-12-22 イルミナ インコーポレイテッド 高スループット単一細胞トランスクリプトームライブラリーならびにその作製方法および使用方法
NL2021258B1 (en) 2018-06-14 2019-12-20 Illumina Inc Device for luminescent imaging
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
US20210332425A1 (en) 2018-08-28 2021-10-28 Rajiv Bharadwaj Increasing spatial array resolution
EP3844308A1 (en) 2018-08-28 2021-07-07 10X Genomics, Inc. Resolving spatial arrays
US12371742B2 (en) * 2018-11-07 2025-07-29 Qingdao MGI Tech Co., Ltd. Method for sequencing polynucleotides
SG11202012762WA (en) 2018-12-05 2021-01-28 Illumina Cambridge Ltd Methods and compositions for cluster generation by bridge amplification
WO2020123318A1 (en) 2018-12-10 2020-06-18 10X Genomics, Inc. Resolving spatial arrays using deconvolution
JP7542444B2 (ja) 2018-12-14 2024-08-30 イルミナ ケンブリッジ リミテッド 配列決定の間のアンラベル化ヌクレオチドによるフェージング減少
JP7296409B2 (ja) 2018-12-17 2023-06-22 イルミナ ケンブリッジ リミテッド ポリヌクレオチドシーケンシングに使用するための組成物
KR102872035B1 (ko) 2018-12-18 2025-10-16 일루미나 케임브리지 리미티드 단일 표면 프라이머를 사용하는 페어드 엔드 서열분석을 위한 방법 및 조성물
US11293061B2 (en) 2018-12-26 2022-04-05 Illumina Cambridge Limited Sequencing methods using nucleotides with 3′ AOM blocking group
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
CN112673034B (zh) 2019-01-14 2023-03-28 伊鲁米那股份有限公司 包含杂聚物的流动池
EP3702474A1 (en) * 2019-02-26 2020-09-02 QIAGEN GmbH Sequencing method and kit
WO2020176788A1 (en) 2019-02-28 2020-09-03 10X Genomics, Inc. Profiling of biological analytes with spatially barcoded oligonucleotide arrays
WO2020178231A1 (en) 2019-03-01 2020-09-10 Illumina, Inc. Multiplexed fluorescent detection of analytes
NL2023327B1 (en) 2019-03-01 2020-09-17 Illumina Inc Multiplexed fluorescent detection of analytes
AU2020232618B2 (en) 2019-03-01 2025-11-13 Illumina, Inc. High-throughput single-nuclei and single-cell libraries and methods of making and of using
IL279674B2 (en) 2019-03-01 2025-04-01 Illumina Cambridge Ltd Amine-modified exocyclic coumarins and their use as fluorescent tags
EP3938538A1 (en) 2019-03-15 2022-01-19 10X Genomics, Inc. Methods for using spatial arrays for single cell sequencing
US11347965B2 (en) 2019-03-21 2022-05-31 Illumina, Inc. Training data generation for artificial intelligence-based sequencing
US11210554B2 (en) 2019-03-21 2021-12-28 Illumina, Inc. Artificial intelligence-based generation of sequencing metadata
WO2020198071A1 (en) 2019-03-22 2020-10-01 10X Genomics, Inc. Three-dimensional spatial analysis
US11421271B2 (en) 2019-03-28 2022-08-23 Illumina Cambridge Limited Methods and compositions for nucleic acid sequencing using photoswitchable labels
US11579217B2 (en) 2019-04-12 2023-02-14 Western Digital Technologies, Inc. Devices and methods for frequency- and phase-based detection of magnetically-labeled molecules using spin torque oscillator (STO) sensors
US11738336B2 (en) 2019-04-12 2023-08-29 Western Digital Technologies, Inc. Spin torque oscillator (STO) sensors used in nucleic acid sequencing arrays and detection schemes for nucleic acid sequencing
US11609208B2 (en) 2019-04-12 2023-03-21 Western Digital Technologies, Inc. Devices and methods for molecule detection based on thermal stabilities of magnetic nanoparticles
US11112468B2 (en) 2019-04-12 2021-09-07 Western Digital Technologies, Inc. Magnetoresistive sensor array for molecule detection and related detection schemes
US12121896B2 (en) 2019-04-12 2024-10-22 Roche Sequencing Solutions, Inc. Nucleic acid sequencing by synthesis using magnetic sensor arrays
US11327073B2 (en) 2019-04-12 2022-05-10 Western Digital Technologies, Inc. Thermal sensor array for molecule detection and related detection schemes
BR112021022809A2 (pt) * 2019-05-15 2022-01-25 Egi Tech Shen Zhen Co Ltd Método de sequenciamento de canal único baseado em autoluminescência
CN117935916A (zh) 2019-05-16 2024-04-26 因美纳有限公司 使用卷积的碱基识别
US11593649B2 (en) 2019-05-16 2023-02-28 Illumina, Inc. Base calling using convolutions
US11423306B2 (en) 2019-05-16 2022-08-23 Illumina, Inc. Systems and devices for characterization and performance analysis of pixel-based sequencing
EP3976820A1 (en) 2019-05-30 2022-04-06 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
CN112703558A (zh) 2019-05-31 2021-04-23 伊鲁米那股份有限公司 用于存储的系统和方法
SG11202012812PA (en) 2019-05-31 2021-01-28 Illumina Inc Flow cell with selective deposition or activation of nucleotides
US11282588B2 (en) 2019-05-31 2022-03-22 Illumina, Inc. Storage device, system, and method
CN112689682A (zh) 2019-05-31 2021-04-20 伊鲁米纳公司 从流动池中的生物样品获取信息
SG11202012811RA (en) 2019-05-31 2021-01-28 Illumina Inc Flow cell with one or more barrier features
WO2020243073A1 (en) 2019-05-31 2020-12-03 Illumina, Inc. Systems and methods for information storage and retrieval using flow cells
CN114981283A (zh) * 2019-07-09 2022-08-30 纽约市哥伦比亚大学信托人 新型核苷酸类似物和使用方法
US20230242979A1 (en) * 2019-08-20 2023-08-03 Egi Tech (Shen Zhen) Co., Limited Method for sequencing polynucleotides based on optical signal kinetics of luminescent labels and secondary luminescent signals
US11208682B2 (en) 2019-09-13 2021-12-28 Western Digital Technologies, Inc. Enhanced optical detection for nucleic acid sequencing using thermally-dependent fluorophore tags
EP4055185A1 (en) 2019-11-08 2022-09-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
EP4025711A2 (en) 2019-11-08 2022-07-13 10X Genomics, Inc. Enhancing specificity of analyte binding
WO2021102039A1 (en) 2019-11-21 2021-05-27 10X Genomics, Inc, Spatial analysis of analytes
US11747329B2 (en) 2019-11-22 2023-09-05 Western Digital Technologies, Inc. Magnetic gradient concentrator/reluctance detector for molecule detection
CA3134746A1 (en) 2019-12-19 2021-06-24 Illumina, Inc. High-throughput single-cell libraries and methods of making and of using
ES2982420T3 (es) 2019-12-23 2024-10-16 10X Genomics Inc Métodos para el análisis espacial mediante el uso de la ligazón con plantilla de ARN
US12241890B2 (en) 2019-12-23 2025-03-04 10X Genomics, Inc. Methods for generating barcoded nucleic acid molecules using fixed cells
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US12112833B2 (en) 2020-02-04 2024-10-08 10X Genomics, Inc. Systems and methods for index hopping filtering
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
WO2021158925A1 (en) 2020-02-07 2021-08-12 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
WO2021163546A1 (en) 2020-02-14 2021-08-19 The Johns Hopkins University Methods and materials for assessing nucleic acids
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
US12215379B2 (en) 2020-02-17 2025-02-04 10X Genomics, Inc. In situ analysis of chromatin interaction
BR112022016415A2 (pt) 2020-02-20 2022-10-11 Illumina Inc Múltiplas chamadas de base à base de inteligência artificial
US20210265016A1 (en) 2020-02-20 2021-08-26 Illumina, Inc. Data Compression for Artificial Intelligence-Based Base Calling
US12354008B2 (en) 2020-02-20 2025-07-08 Illumina, Inc. Knowledge distillation and gradient pruning-based compression of artificial intelligence-based base caller
EP4107285B1 (en) 2020-02-21 2024-10-09 10X Genomics, Inc. Methods and compositions for integrated in situ spatial assay
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US12188085B2 (en) 2020-03-05 2025-01-07 10X Genomics, Inc. Three-dimensional spatial transcriptomics with sequencing readout
GB2608318A (en) * 2020-03-13 2022-12-28 Apton Biosystems Inc Densely-packed analyte layers and detection methods
WO2021188717A1 (en) * 2020-03-18 2021-09-23 Apton Biosystems, Inc. Systems and methods of detecting densely-packed analytes
EP4139052A4 (en) * 2020-04-21 2023-10-18 Roche Diagnostics GmbH High-throughput nucleic acid sequencing with single-molecule sensor arrays
WO2021216708A1 (en) 2020-04-22 2021-10-28 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
US11188778B1 (en) 2020-05-05 2021-11-30 Illumina, Inc. Equalization-based image processing and spatial crosstalk attenuator
AU2021271637A1 (en) 2020-05-12 2022-12-08 Illumina Singapore Pte. Ltd. Generating nucleic acids with modified bases using recombinant terminal deoxynucleotidyl transferase
AU2021275906A1 (en) 2020-05-22 2022-12-22 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021236929A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
WO2021252617A1 (en) 2020-06-09 2021-12-16 Illumina, Inc. Methods for increasing yield of sequencing libraries
EP4165207B1 (en) 2020-06-10 2024-09-25 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
BR112022026056A2 (pt) * 2020-06-22 2023-03-07 Illumina Cambridge Ltd Nucleosídeos e nucleotídeos com grupo bloqueador 3? acetal
AU2021294334A1 (en) 2020-06-25 2023-02-02 10X Genomics, Inc. Spatial analysis of DNA methylation
JP2023532231A (ja) 2020-06-30 2023-07-27 イルミナ インコーポレイテッド 傷なしｄｎａを生成するための触媒的に制御された合成による配列決定
US12168801B1 (en) 2020-07-02 2024-12-17 10X Genomics, Inc. Hybrid/capture probe designs for full-length cDNA
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
CN116018412A (zh) 2020-07-08 2023-04-25 Illumina公司 作为转座体载体的小珠
US11981964B2 (en) 2020-07-28 2024-05-14 Illumina Cambridge Limited Substituted coumarin dyes and uses as fluorescent labels
EP4121557A4 (en) 2020-08-06 2024-05-01 Singular Genomics Systems, Inc. Spatial sequencing
US11492662B2 (en) 2020-08-06 2022-11-08 Singular Genomics Systems, Inc. Methods for in situ transcriptomics and proteomics
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
WO2022094415A1 (en) 2020-10-30 2022-05-05 Singular Genomics Systems, Inc. Methods and compositions for reducing nucleotide impurities
US12071667B2 (en) 2020-11-04 2024-08-27 10X Genomics, Inc. Sequence analysis using meta-stable nucleic acid molecules
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
EP4012046A1 (en) 2020-12-11 2022-06-15 10X Genomics, Inc. Methods and compositions for multimodal in situ analysis
GB2602063A (en) * 2020-12-17 2022-06-22 Sumitomo Chemical Co Sequencing method
US20220195516A1 (en) * 2020-12-17 2022-06-23 Illumina Cambridge Limited Methods, systems and compositions for nucleic acid sequencing
WO2022140028A1 (en) 2020-12-21 2022-06-30 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
US20220195518A1 (en) * 2020-12-22 2022-06-23 Illumina Cambridge Limited Methods and compositions for nucleic acid sequencing
US12060603B2 (en) 2021-01-19 2024-08-13 10X Genomics, Inc. Methods for internally controlled in situ assays using padlock probes
EP4284945B1 (en) 2021-01-26 2024-10-23 10X Genomics, Inc. Nucleic acid analog probes for in situ analysis
WO2022187366A1 (en) 2021-03-03 2022-09-09 10X Genomics, Inc. Analyte detection in situ using nucleic acid origami
WO2022197754A1 (en) 2021-03-16 2022-09-22 Illumina Software, Inc. Neural network parameter quantization for base calling
CN117043867B (zh) 2021-04-02 2025-11-28 因美纳有限公司 用于检测用于测序的核苷酸样品玻片内的气泡的机器学习模型
EP4305196B1 (en) 2021-04-14 2025-04-02 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
US12217829B2 (en) 2021-04-15 2025-02-04 Illumina, Inc. Artificial intelligence-based analysis of protein three-dimensional (3D) structures
US12444482B2 (en) 2021-04-15 2025-10-14 Illumina, Inc. Multi-channel protein voxelization to predict variant pathogenicity using deep convolutional neural networks
EP4334706A4 (en) 2021-05-05 2025-08-06 Singular Genomics Systems Inc MULTIOMICS ANALYSIS DEVICE AND METHODS OF USE THEREOF
EP4320271B1 (en) 2021-05-06 2025-03-19 10X Genomics, Inc. Methods for increasing resolution of spatial analysis
CN117916390A (zh) 2021-05-20 2024-04-19 伊鲁米纳公司 用于边合成边测序的组合物和方法
CN117396613A (zh) 2021-06-01 2024-01-12 10X基因组学有限公司 用于分析物检测和探针解析的方法和组合物
WO2022256422A1 (en) 2021-06-02 2022-12-08 10X Genomics, Inc. Sample analysis using asymmetric circularizable probes
WO2022256503A1 (en) 2021-06-03 2022-12-08 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
WO2022272260A1 (en) 2021-06-23 2022-12-29 Illumina, Inc. Compositions, methods, kits, cartridges, and systems for sequencing reagents
CN117651605A (zh) 2021-06-25 2024-03-05 伊鲁米纳公司 在图案化表面的配准中使用的基准
AU2022296802A1 (en) 2021-06-25 2024-01-04 Illumina, Inc. Linear fourier fiducial
WO2023278927A1 (en) 2021-06-29 2023-01-05 Illumina Software, Inc. Signal-to-noise-ratio metric for determining nucleotide-base calls and base-call quality
WO2023278184A1 (en) * 2021-06-29 2023-01-05 Illumina, Inc. Methods and systems to correct crosstalk in illumination emitted from reaction sites
US20220415443A1 (en) 2021-06-29 2022-12-29 Illumina, Inc. Machine-learning model for generating confidence classifications for genomic coordinates
JP2024527306A (ja) 2021-06-29 2024-07-24 イルミナ インコーポレイテッド オリゴ配列を使用して訓練された自己学習ベースコーラ
US12423815B2 (en) 2021-07-13 2025-09-23 Illumina, Inc. Methods and systems for real time extraction of crosstalk in illumination emitted from reaction sites
EP4370896A1 (en) 2021-07-13 2024-05-22 10X Genomics, Inc. Methods for preparing polymerized matrix with controllable thickness
US11455487B1 (en) 2021-10-26 2022-09-27 Illumina Software, Inc. Intensity extraction and crosstalk attenuation using interpolation and adaptation for base calling
JP2024532004A (ja) 2021-07-19 2024-09-04 イルミナ インコーポレイテッド ベースコールのための補間及び適合による強度抽出
US20230021577A1 (en) 2021-07-23 2023-01-26 Illumina Software, Inc. Machine-learning model for recalibrating nucleotide-base calls
EP4377960A1 (en) 2021-07-28 2024-06-05 Illumina, Inc. Quality score calibration of basecalling systems
WO2023009842A1 (en) 2021-07-30 2023-02-02 10X Genomics, Inc. Methods and compositions for synchronizing reactions in situ
US12139751B2 (en) 2021-07-30 2024-11-12 10X Genomics, Inc. Circularizable probes for in situ analysis
WO2023015192A1 (en) 2021-08-03 2023-02-09 10X Genomics, Inc. Nucleic acid concatemers and methods for stabilizing and/or compacting the same
US12460251B2 (en) 2021-08-03 2025-11-04 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid molecules
EP4381514A1 (en) 2021-08-03 2024-06-12 Illumina, Inc. Base calling using multiple base caller models
US12391984B2 (en) 2021-08-03 2025-08-19 10X Genomics, Inc. Compositions and methods for rolling circle amplification
US12435364B2 (en) 2021-08-16 2025-10-07 10X Genomics, Inc. Probes comprising a split barcode region and methods of use
JP2024534741A (ja) 2021-09-01 2024-09-26 イルミナ インコーポレイテッド 加速した塩基呼び出しのための振幅変調
EP4196605B1 (en) 2021-09-01 2024-12-04 10X Genomics, Inc. Methods for blocking a capture probe on a spatial array
JP2024535663A (ja) 2021-09-17 2024-10-02 イルミナ インコーポレイテッド ヌクレオチド配列決定における塩基コールエラーパターンからの障害ソースの自動的特定
CN117546243A (zh) 2021-09-21 2024-02-09 因美纳有限公司 使用推算的单倍型的图参考基因组和碱基检出方法
US12480157B2 (en) 2021-09-30 2025-11-25 Illumina, Inc. Polynucleotide sequencing
EP4419707A1 (en) 2021-11-10 2024-08-28 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
WO2023102354A1 (en) 2021-12-02 2023-06-08 Illumina Software, Inc. Generating cluster-specific-signal corrections for determining nucleotide-base calls
WO2023107622A1 (en) 2021-12-10 2023-06-15 Illumina, Inc. Parallel sample and index sequencing
WO2023122363A1 (en) 2021-12-23 2023-06-29 Illumina Software, Inc. Dynamic graphical status summaries for nucelotide sequencing
US20230215515A1 (en) 2021-12-23 2023-07-06 Illumina Software, Inc. Facilitating secure execution of external workflows for genomic sequencing diagnostics
EP4423296A2 (en) 2021-12-27 2024-09-04 10X Genomics, Inc. Methods and compositions for rolling circle amplification
US20230207050A1 (en) 2021-12-28 2023-06-29 Illumina Software, Inc. Machine learning model for recalibrating nucleotide base calls corresponding to target variants
KR20240131386A (ko) 2021-12-29 2024-08-30 일루미나, 인코포레이티드 유전체 분석 애플리케이션을 위한 변이 분석 모델 버전 자동 스위칭
US12497653B2 (en) 2022-01-21 2025-12-16 10X Genomics, Inc. Multiple readout signals for analyzing a sample
EP4482983A1 (en) 2022-02-23 2025-01-01 Insitro, Inc. Pooled optical screening and transcriptional measurements of cells comprising barcoded genetic perturbations
KR20240152324A (ko) 2022-02-25 2024-10-21 일루미나, 인코포레이티드 뉴클레오티드 서열분석을 위한 교정 서열
WO2023164492A1 (en) 2022-02-25 2023-08-31 Illumina, Inc. Machine-learning models for detecting and adjusting values for nucleotide methylation levels
EP4341425A2 (en) 2022-03-15 2024-03-27 Illumina, Inc. Concurrent sequencing of forward and reverse complement strands on concatenated polynucleotides for methylation detection
WO2023175021A1 (en) 2022-03-15 2023-09-21 Illumina, Inc. Methods of preparing loop fork libraries
AU2023246676A1 (en) * 2022-03-28 2024-01-18 Illumina Inc. Labeled avidin and methods for sequencing
US20230313292A1 (en) 2022-03-29 2023-10-05 Illumina Cambridge Limited Chromenoquinoline dyes and uses in sequencing
AU2023243205A1 (en) 2022-04-01 2024-09-19 10X Genomics, Inc. Compositions and methods for targeted masking of autofluorescence
EP4504969A1 (en) 2022-04-06 2025-02-12 10X Genomics, Inc. Methods for multiplex cell analysis
JP2025512975A (ja) 2022-04-07 2025-04-22 イルミナ インコーポレイテッド 改変シチジンデアミナーゼ及び使用方法
EP4515547A1 (en) 2022-04-26 2025-03-05 Illumina, Inc. Machine-learning models for selecting oligonucleotide probes for array technologies
US20230348967A1 (en) 2022-04-29 2023-11-02 Illumina Cambridge Limited Methods and systems for encapsulating lyophilised microspheres
WO2023215612A1 (en) 2022-05-06 2023-11-09 10X Genomics, Inc. Analysis of antigen and antigen receptor interactions
US20230368866A1 (en) 2022-05-10 2023-11-16 Illumina Software, Inc. Adaptive neural network for nucelotide sequencing
JP2025516589A (ja) 2022-05-11 2025-05-30 １０エックス ジェノミクス インコーポレイテッド インサイチュー配列決定のための組成物及び方法
US20230383342A1 (en) 2022-05-31 2023-11-30 Illumina Cambridge Limited Compositions and methods for nucleic acid sequencing
EP4540607A1 (en) 2022-06-17 2025-04-23 10X Genomics, Inc. Catalytic de-crosslinking of samples for in situ analysis
CA3260493A1 (en) 2022-06-24 2023-12-28 Illumina, Inc. IMPROVING DIVISION READING ALIGNMENT BY INTELLIGENTLY IDENTIFYING AND EVALUATING CANDIDATE DIVISION GROUPS
WO2024006705A1 (en) 2022-06-27 2024-01-04 Illumina Software, Inc. Improved human leukocyte antigen (hla) genotyping
JP2025523560A (ja) 2022-06-27 2025-07-23 イルミナ インコーポレイテッド 遺伝子型帰属モデルのための加速器
JP2025523561A (ja) 2022-06-27 2025-07-23 イルミナ インコーポレイテッド 構造多型グラフゲノムの生成及び実装
US20240038327A1 (en) 2022-07-26 2024-02-01 Illumina Software, Inc. Rapid single-cell multiomics processing using an executable file
US20240033738A1 (en) 2022-07-27 2024-02-01 Illumina, Inc. Flow cell based motion system calibration and control methods
WO2024036304A1 (en) 2022-08-12 2024-02-15 10X Genomics, Inc. Puma1 polymerases and uses thereof
AU2023325054A1 (en) 2022-08-15 2025-02-27 Element Biosciences, Inc. Methods for preparing nucleic acid nanostructures using compaction oligonucleotides
EP4511510B1 (en) 2022-08-16 2025-12-10 10X Genomics, Inc. Ap50 polymerases and uses thereof
WO2024039516A1 (en) 2022-08-19 2024-02-22 Illumina, Inc. Third dna base pair site-specific dna detection
WO2024059852A1 (en) 2022-09-16 2024-03-21 Illumina, Inc. Cluster segmentation and conditional base calling
US20250230496A1 (en) 2022-09-19 2025-07-17 Illumina, Inc. Deformable polymers comprising immobilised primers
US20240100518A1 (en) 2022-09-26 2024-03-28 Illumina, Inc. Flow cell based motion system calibration and control methods
US20240112753A1 (en) 2022-09-29 2024-04-04 Illumina, Inc. Target-variant-reference panel for imputing target variants
JP2025534192A (ja) 2022-09-30 2025-10-15 イルミナ インコーポレイテッド 構造バリアントコールを精緻化するための機械学習モデル
CA3246518A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Compositions and methods for reducing photolesions during sequencing
WO2024069581A1 (en) 2022-09-30 2024-04-04 Illumina Singapore Pte. Ltd. Helicase-cytidine deaminase complexes and methods of use
EP4594482A1 (en) 2022-09-30 2025-08-06 Illumina, Inc. Cytidine deaminases and methods of use in mapping modified cytosine nucleotides
EP4594343A1 (en) 2022-09-30 2025-08-06 Illumina, Inc. Methods of using cpg binding proteins in mapping modified cytosine nucleotides
KR20250081825A (ko) 2022-10-05 2025-06-05 일루미나, 인코포레이티드 기계 학습 아키텍처를 활용하는 다중 서열분석 파이프라인의 변이 호출 통합
WO2024081649A1 (en) 2022-10-11 2024-04-18 Illumina, Inc. Detecting and correcting methylation values from methylation sequencing assays
EP4602344A1 (en) 2022-10-14 2025-08-20 10X Genomics, Inc. Methods for analysis of biological samples
US20240167081A1 (en) 2022-11-08 2024-05-23 10X Genomics,Inc. Immobilization methods and compositions for in situ detection
EP4573210A1 (en) 2022-11-16 2025-06-25 10X Genomics, Inc. Methods and compositions for assessing performance of in situ assays
EP4619736A1 (en) 2022-11-18 2025-09-24 10X Genomics, Inc. Systems and methods for actively mitigating vibrations
EP4627583A1 (en) 2022-11-30 2025-10-08 Illumina, Inc. Accurately predicting variants from methylation sequencing data
EP4634404A1 (en) 2022-12-16 2025-10-22 10X Genomics, Inc. Methods and compositions for assessing performance
WO2024137860A1 (en) 2022-12-21 2024-06-27 10X Genomics, Inc. Methods for tethering ribonucleic acids in biological samples
WO2024137886A1 (en) 2022-12-21 2024-06-27 Illumina, Inc. Context-dependent base calling
CN119095983A (zh) 2022-12-22 2024-12-06 伊路米纳有限公司 用于边合成边测序的过渡金属催化剂组合物和方法
CN119095982A (zh) 2022-12-22 2024-12-06 伊路米纳有限公司 用于边合成边测序的钯催化剂组合物和方法
CN119095984A (zh) 2022-12-27 2024-12-06 伊路米纳有限公司 使用3'烯丙基封端的核苷酸的测序方法
US20240263219A1 (en) 2023-01-06 2024-08-08 10X Genomics, Inc. Methods and compositions for in situ analysis of variant sequences
KR20250144883A (ko) 2023-02-06 2025-10-13 일루미나, 인코포레이티드 클러스터 간 광 간섭 결정 및 제거
WO2024206848A1 (en) 2023-03-30 2024-10-03 Illumina, Inc. Tandem repeat genotyping
AU2024247902A1 (en) 2023-03-30 2024-12-12 Illumina, Inc. Compositions and methods for nucleic acid sequencing
WO2024216159A1 (en) * 2023-04-13 2024-10-17 Esbiolab Llc Methods and compositions for nucleic acid sequencing using labeled nucleotides
WO2024216163A1 (en) * 2023-04-13 2024-10-17 Esbiolab Llc Methods and compositions for nucleic acid sequencing using predominantly unlabeled nucleotides
US20240368678A1 (en) 2023-05-03 2024-11-07 10X Genomics, Inc. Methods and compositions for spatial assay
IL317962A (en) 2023-05-03 2025-02-01 Illumina Inc A machine learning model for recalibrating genotype reads from existing sequence datasets
WO2024249200A1 (en) 2023-05-26 2024-12-05 Illumina, Inc. Methods for preserving methylation status during clustering
US20240404624A1 (en) 2023-05-31 2024-12-05 Illumina, Inc. Structural variant alignment and variant calling by utilizing a structural-variant reference genome
WO2024249961A1 (en) 2023-06-01 2024-12-05 Singular Genomics Systems, Inc. Methods and probes for detecting polynucleotide sequences in cells and tissues
WO2024249973A2 (en) 2023-06-02 2024-12-05 Illumina, Inc. Linking human genes to clinical phenotypes using graph neural networks
WO2024256581A1 (en) 2023-06-14 2024-12-19 Illumina, Inc. Determination of modified cytosines
WO2024256580A1 (en) 2023-06-14 2024-12-19 Illumina, Inc. Concurrent sequencing with spatially separated rings
WO2025006565A1 (en) 2023-06-27 2025-01-02 Illumina, Inc. Variant calling with methylation-level estimation
WO2025006460A1 (en) * 2023-06-30 2025-01-02 Illumina, Inc. Systems and methods of sequencing polynucleotides with modified bases
WO2025006874A1 (en) 2023-06-30 2025-01-02 Illumina, Inc. Machine-learning model for recalibrating genotype calls corresponding to germline variants and somatic mosaic variants
WO2025029831A1 (en) 2023-07-31 2025-02-06 10X Genomics, Inc. Methods and systems for targeted rna cleavage and target rna-primed rolling circle amplification
WO2025054389A1 (en) 2023-09-07 2025-03-13 Illumina, Inc. Identification of methylated cytosine using landmarks
EP4520821A1 (en) 2023-09-08 2025-03-12 The Regents Of The University Of Michigan Microrna-derived rnas and polypeptides and uses thereof
WO2025062002A1 (en) 2023-09-20 2025-03-27 Illumina, Inc. Concurrent sequencing using nick translation
WO2025061942A1 (en) 2023-09-20 2025-03-27 Illumina, Inc. Sequencing error identification and correction
WO2025062001A1 (en) 2023-09-20 2025-03-27 Illumina, Inc. Optimised nucleic acid sequencing
WO2025061922A1 (en) 2023-09-20 2025-03-27 Illumina, Inc. Methods for sequencing
WO2025072793A1 (en) 2023-09-28 2025-04-03 Illumina, Inc. Altered cytidine deaminases and methods of use
WO2025072870A1 (en) 2023-09-29 2025-04-03 Illumina, Inc. Tracking and modifying cluster location on nucleotide-sample slides in real time
US20250111899A1 (en) 2023-09-29 2025-04-03 Illumina, Inc. Predicting insert lengths using primary analysis metrics
WO2025081064A2 (en) 2023-10-11 2025-04-17 Illumina, Inc. Thermophilic deaminase and methods for identifying modified cytosine
WO2025090883A1 (en) 2023-10-27 2025-05-01 Illumina, Inc. Detecting variants in nucleotide sequences based on haplotype diversity
WO2025111463A1 (en) 2023-11-22 2025-05-30 10X Genomics, Inc. Methods for processing ribonucleic acids in biological samples
WO2025129133A1 (en) 2023-12-15 2025-06-19 Illumina, Inc. Minimal residual disease (mrd) models for determining likelihoods or probabilities of a subject comprising cancer
WO2025136998A1 (en) 2023-12-18 2025-06-26 Illumina, Inc. Using machine learning models for detecting minimum residual disease (mrd) in a subject
WO2025137222A1 (en) 2023-12-19 2025-06-26 Illumina, Inc. Methylation detection assay
WO2025137341A1 (en) 2023-12-20 2025-06-26 Illumina, Inc. Directly determining signal-to-noise-ratio metrics for accelerated convergence in determining nucleotide-base calls and base-call quality
EP4578960A1 (en) 2023-12-20 2025-07-02 10x Genomics, Inc. In situ detection of copy number variations in biological samples
US20250210141A1 (en) 2023-12-21 2025-06-26 Illumina, Inc. Enhanced mapping and alignment of nucleotide reads utilizing an improved haplotype data structure with allele-variant differences
WO2025144626A1 (en) 2023-12-28 2025-07-03 Illumina, Inc. Sequencing with single-stranded binding proteins
WO2025144716A1 (en) 2023-12-28 2025-07-03 Illumina, Inc. Nucleotides with enzymatically cleavable 3'-o-glycoside blocking groups for sequencing
WO2025160089A1 (en) 2024-01-26 2025-07-31 Illumina, Inc. Custom multigenome reference construction for improved sequencing analysis of genomic samples
WO2025174774A1 (en) 2024-02-12 2025-08-21 Illumina, Inc. Determining offline corrections for sequence specific errors caused by low complexity nucleotide sequences
WO2025174708A1 (en) 2024-02-13 2025-08-21 Illumina, Inc. Design and method for cross-sequencing platform compatibility of flow cells
WO2025184226A1 (en) 2024-02-28 2025-09-04 Illumina, Inc. Nucleotides with terminal phosphate capping
WO2025184234A1 (en) 2024-02-28 2025-09-04 Illumina, Inc. A personalized haplotype database for improved mapping and alignment of nucleotide reads and improved genotype calling
WO2025188906A1 (en) 2024-03-08 2025-09-12 Illumina, Inc. Modified adenosine nucleotides
WO2025193747A1 (en) 2024-03-12 2025-09-18 Illumina, Inc. Machine-learning models for ordering and expediting sequencing tasks or corresponding nucleotide-sample slides
WO2025190902A1 (en) 2024-03-13 2025-09-18 Illumina, Inc. Improving base calling quality scores
US20250288988A1 (en) 2024-03-15 2025-09-18 10X Genomics, Inc. Systems and methods for covering and sealing an open well
WO2025230914A1 (en) 2024-04-29 2025-11-06 Illumina, Inc. Nucleotides with enzyme-triggered self-immolative linkers for sequencing by synthesis
WO2025240241A1 (en) 2024-05-13 2025-11-20 Illumina, Inc. Modifying sequencing cycles during a sequencing run to meet customized coverage estimations for a target genomic region
WO2025240918A1 (en) 2024-05-17 2025-11-20 10X Genomics, Inc. Systems and methods for generating codebooks
WO2025240924A1 (en) 2024-05-17 2025-11-20 Illumina, Inc. Blind equalization systems for base calling applications
WO2025250996A2 (en) 2024-05-31 2025-12-04 Illumina, Inc. Call generation and recalibration models for implementing personalized diploid reference haplotypes in genotype calling
WO2025255457A1 (en) 2024-06-07 2025-12-11 10X Genomics, Inc. Compositions and methods for improved on-target spatial profiling

Family Cites Families (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US781650A (en) 1904-08-15 1905-02-07 William Heap Commutator-brush holder.
US6051380A (en) 1993-11-01 2000-04-18 Nanogen, Inc. Methods and procedures for molecular biological analysis and diagnostics
US5641658A (en) 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
US5695934A (en) 1994-10-13 1997-12-09 Lynx Therapeutics, Inc. Massively parallel sequencing of sorted polynucleotides
WO1998044151A1 (en) 1997-04-01 1998-10-08 Glaxo Group Limited Method of nucleic acid amplification
DE69824716D1 (de) 1997-04-01 2004-07-29 Manteia S A Methode zur sequenzierung von nukleinsäuren
US5888737A (en) 1997-04-15 1999-03-30 Lynx Therapeutics, Inc. Adaptor-based sequence analysis
EP0994960A1 (en) 1997-06-25 2000-04-26 Orchid Biocomputer, Inc. Methods for the detection of multiple single nucleotide polymorphisms in a single reaction
EP1019496B1 (en) 1997-07-07 2004-09-29 Medical Research Council In vitro sorting method
JP3662850B2 (ja) 1998-06-24 2005-06-22 イルミナ インコーポレイテッド 微小球を有するアレイセンサーのデコード
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US7071324B2 (en) 1998-10-13 2006-07-04 Brown University Research Foundation Systems and methods for sequencing by hybridization
NO986133D0 (no) 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
EP1218543A2 (en) * 1999-09-29 2002-07-03 Solexa Ltd. Polynucleotide sequencing
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
WO2002072892A1 (en) * 2001-03-12 2002-09-19 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
GB0129012D0 (en) 2001-12-04 2002-01-23 Solexa Ltd Labelled nucleotides
US20040002090A1 (en) 2002-03-05 2004-01-01 Pascal Mayer Methods for detecting genome-wide sequence variations associated with a phenotype
EP1497304B1 (en) * 2002-04-12 2014-06-25 Catalyst Assets LLC Dual-labeled nucleotides
US7414116B2 (en) * 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
US7541444B2 (en) 2002-08-23 2009-06-02 Illumina Cambridge Limited Modified nucleotides
PT3147292T (pt) 2002-08-23 2018-11-22 Illumina Cambridge Ltd Nucleótidos identificados
CN102212614B (zh) 2003-01-29 2013-12-25 454生命科学公司 核酸扩增和测序的方法
US20050181394A1 (en) 2003-06-20 2005-08-18 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
EP1641809B2 (en) 2003-07-05 2018-10-03 The Johns Hopkins University Method and compositions for detection and enumeration of genetic variations
GB0324456D0 (en) * 2003-10-20 2003-11-19 Isis Innovation Parallel DNA sequencing methods
US7276338B2 (en) 2003-11-17 2007-10-02 Jacobson Joseph M Nucleotide sequencing via repetitive single molecule hybridization
ES2602732T3 (es) 2004-04-13 2017-02-22 Biosearch Technologies, Inc. Colorantes de xanteno
EP1879906A4 (en) * 2005-01-05 2009-07-22 Advanced Genetic Analysis Corp REVERSIBLE NUCLEOTIDE TERMINATORS AND USES THEREOF
GB0507835D0 (en) * 2005-04-18 2005-05-25 Solexa Ltd Method and device for nucleic acid sequencing using a planar wave guide
CN101466847B (zh) 2005-06-15 2014-02-19 考利达基因组股份有限公司 用于遗传和化学分析的单分子阵列
US20060287833A1 (en) 2005-06-17 2006-12-21 Zohar Yakhini Method and system for sequencing nucleic acid molecules using sequencing by hybridization and comparison with decoration patterns
US7514952B2 (en) 2005-06-29 2009-04-07 Altera Corporation I/O circuitry for reducing ground bounce and VCC sag in integrated circuit devices
GB0517097D0 (en) 2005-08-19 2005-09-28 Solexa Ltd Modified nucleosides and nucleotides and uses thereof
AU2007249635B2 (en) 2005-10-07 2012-05-31 Complete Genomics, Inc. High throughput genome sequencing on DNA arrays
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
SG170028A1 (en) 2006-02-24 2011-04-29 Callida Genomics Inc High throughput genome sequencing on dna arrays
WO2007107710A1 (en) 2006-03-17 2007-09-27 Solexa Limited Isothermal methods for creating clonal single molecule arrays
JP5122555B2 (ja) 2006-03-31 2013-01-16 ソレクサ・インコーポレイテッド 合成解析による配列決定システムおよび装置
EP2021415B1 (en) 2006-05-18 2017-03-15 Illumina Cambridge Limited Dye compounds and the use of their labelled conjugates
JP2009542220A (ja) * 2006-06-30 2009-12-03 アプライド バイオシステムズ， エルエルシー 可逆性ターミネーターヌクレオチドおよび使用方法
US20100304368A1 (en) * 2006-09-20 2010-12-02 Dmitry Cherkasov Components and method for enzymatic synthesis of nucleic acids
WO2008042067A2 (en) * 2006-09-28 2008-04-10 Illumina, Inc. Compositions and methods for nucleotide sequencing
WO2008070352A2 (en) 2006-10-27 2008-06-12 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
EP2126765B1 (en) * 2007-01-26 2011-08-24 Illumina Inc. Nucleic acid sequencing system and method
DK2725107T3 (da) * 2007-10-19 2019-01-02 Univ Columbia DNA-sekventering med ikke-fluorescerende nukleotidreversible terminatorer og ddNTP'er modificeret med spaltbart mærke og nukleinsyre omfattende inosin med reversible terminatorer
US20110014611A1 (en) * 2007-10-19 2011-01-20 Jingyue Ju Design and synthesis of cleavable fluorescent nucleotides as reversible terminators for dna sequences by synthesis
US8617811B2 (en) * 2008-01-28 2013-12-31 Complete Genomics, Inc. Methods and compositions for efficient base calling in sequencing reactions
US7771903B2 (en) 2008-05-28 2010-08-10 Promos Technologies Pte. Ltd. Photolithography with optical masks having more transparent features surrounded by less transparent features
US20100092960A1 (en) 2008-07-25 2010-04-15 Pacific Biosciences Of California, Inc. Helicase-assisted sequencing with molecular beacons
US8772473B2 (en) * 2009-03-30 2014-07-08 The Regents Of The University Of California Mostly natural DNA sequencing by synthesis
CN101565746B (zh) 2009-06-03 2012-08-08 东南大学 带奇偶校验的信号组合编码dna连接测序方法
CN101570784B (zh) * 2009-06-03 2011-11-23 东南大学 基于信号组合编码的dna连接测序方法
US9309569B2 (en) * 2010-08-26 2016-04-12 Intelligent Bio-Systems, Inc. Methods and compositions for sequencing nucleic acid using charge
DK2768972T3 (en) * 2011-09-23 2017-09-25 Illumina Inc Methods and Compositions for Nucleic Acid Sequencing
WO2013166305A1 (en) 2012-05-02 2013-11-07 Ibis Biosciences, Inc. Dna sequencing

Also Published As

Publication number Publication date
EP3623481A1 (en) 2020-03-18
WO2013044018A1 (en) 2013-03-28
CA2859660A1 (en) 2013-03-28
EP2768972B1 (en) 2017-07-12
DK3623481T3 (da) 2021-11-15
CA3104322A1 (en) 2013-03-28
ES2639938T5 (es) 2021-05-07
US20190264279A1 (en) 2019-08-29
LT3623481T (lt) 2021-09-27
US10287629B2 (en) 2019-05-14
PT3290528T (pt) 2019-10-14
EP3981886A1 (en) 2022-04-13
DK3290528T3 (da) 2019-11-04
EP2768972A1 (en) 2014-08-27
SMT202100624T1 (it) 2022-01-10
DK2768972T3 (en) 2017-09-25
SI3623481T1 (sl) 2022-01-31
PL3623481T3 (pl) 2022-01-17
US11827932B2 (en) 2023-11-28
CY1125261T1 (el) 2023-03-24
US20130079232A1 (en) 2013-03-28
US10900077B2 (en) 2021-01-26
PT3623481T (pt) 2021-10-15
HK1247254A1 (en) 2018-09-21
ES2639938T3 (es) 2017-10-30
US20210139978A1 (en) 2021-05-13
US9453258B2 (en) 2016-09-27
US20240141428A1 (en) 2024-05-02
RS62530B1 (sr) 2021-11-30
ES2895184T3 (es) 2022-02-17
US20170022559A1 (en) 2017-01-26
ES2752653T3 (es) 2020-04-06
EP3623481B1 (en) 2021-08-25
CA3104322C (en) 2023-06-13
EP2768972B2 (en) 2020-07-22
CA2859660C (en) 2021-02-09
EP3290528B1 (en) 2019-08-14
HUE056246T2 (hu) 2022-02-28
HK1201079A1 (en) 2015-08-21
HRP20211523T1 (hr) 2021-12-24
EP3290528A1 (en) 2018-03-07

Similar Documents

Publication Publication Date Title
PT3623481T (pt) Composições para sequenciação de ácidos nucleicos
IL293796A (en) Methods for high-throughput nucleic acid assembly and sequencing
GB2499340B (en) Methods for sequencing nucleic acids
SG10201510189WA (en) Compositions And Methods For Directional Nucleic Acid Amplification And Sequencing
EP2890966A4 (en) PROCESS AND KIT FOR CLEANING NUCLEIC ACIDS
EP2831283A4 (en) METHODS AND COMPOSITION FOR SEQUENCING MODIFIED NUCLEIC ACIDS
EP2694679A4 (en) METHODS AND SYSTEMS FOR LONG NUCLEIC ACID SEQUENCING
PT2591125T (pt) Estratégias 3-d de sequenciação de regiões genómicas de interesse
ZA201205891B (en) Dna polymerases
SG10201606482TA (en) Compositions and methods relating to nucleic acid nano-and micro-technology
GB201212637D0 (en) Nucleic acid amplification
GB2503856B (en) Methods for nucleic acid purification
IL223257A0 (en) Method of dna sequencing by hybridisation
GB201119903D0 (en) Nucleic acid production and sequence analysis
LT2993232T (lt) Nukleorūgščių gavimo būdas
EP2798092A4 (en) nucleic acid hybridization probes
EP2740804A4 (en) PROCESS FOR NUCLEIC ACID ANALYSIS
IL226170B (en) Compositions and methods for delivery of nucleic acids based on nanopolymers
HUE038608T2 (hu) Kompozíciók és eljárások nukleinsav-hibridizáltatáshoz
DK3511424T3 (en) Compositions and methods for improving nanopore sequencing
EP2594592A4 (en) COMPLEX (NUCLEIC ACID) -POLYSACCHARIDE
GB201107286D0 (en) Nucleic acid preparation
GB201118815D0 (en) Methods and compositions for identifying nucleic acids
GB201118795D0 (en) Methods and compositions for identifying nucleic acids
GB201107644D0 (en) Methods and compositions for identifying nucleic acids