PL2292754T3 - Wysokowydajny transfer genu i ekspresja w komórkach ssaczych przez procedurę wielokrotnej transfekcji sekwencji regionu przylegania do macierzy - Google Patents

Wysokowydajny transfer genu i ekspresja w komórkach ssaczych przez procedurę wielokrotnej transfekcji sekwencji regionu przylegania do macierzy

Info

Publication number
PL2292754T3
PL2292754T3 PL10178871T PL10178871T PL2292754T3 PL 2292754 T3 PL2292754 T3 PL 2292754T3 PL 10178871 T PL10178871 T PL 10178871T PL 10178871 T PL10178871 T PL 10178871T PL 2292754 T3 PL2292754 T3 PL 2292754T3
Authority
PL
Poland
Prior art keywords
expression
sequence
high efficiency
mammalian cells
gene transfer
Prior art date
Application number
PL10178871T
Other languages
English (en)
Polish (pl)
Inventor
Nicolas Mermod
Pierre Alain Girod
Philipp Bucher
David Calabrese
Duc-Quang Nguyen
Damien Saugy
Stefania Puttini
Original Assignee
Selexis Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Selexis Sa filed Critical Selexis Sa
Publication of PL2292754T3 publication Critical patent/PL2292754T3/pl

Links

Classifications

    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B5/00ICT specially adapted for modelling or simulations in systems biology, e.g. gene-regulatory networks, protein interaction networks or metabolic networks
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1089Design, preparation, screening or analysis of libraries using computer algorithms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B15/00ICT specially adapted for analysing two-dimensional or three-dimensional molecular structures, e.g. structural or functional relations or structure alignment
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00ICT specially adapted for sequence analysis involving nucleotides or amino acids

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Medical Informatics (AREA)
  • Evolutionary Biology (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
PL10178871T 2003-10-24 2004-10-22 Wysokowydajny transfer genu i ekspresja w komórkach ssaczych przez procedurę wielokrotnej transfekcji sekwencji regionu przylegania do macierzy PL2292754T3 (pl)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US51357403P 2003-10-24 2003-10-24
EP04002722 2004-02-06
EP08153753A EP1959011B1 (en) 2003-10-24 2004-10-22 High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of matrix attachment region sequences
EP04790766A EP1675952A2 (en) 2003-10-24 2004-10-22 High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of matrix attachment region sequences
EP10178871A EP2292754B1 (en) 2003-10-24 2004-10-22 High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of matrix attachment region sequences

Publications (1)

Publication Number Publication Date
PL2292754T3 true PL2292754T3 (pl) 2013-06-28

Family

ID=34524793

Family Applications (4)

Application Number Title Priority Date Filing Date
PL08153753T PL1959011T3 (pl) 2003-10-24 2004-10-22 Wysokowydajny transfer genu i ekspresja w komórkach ssaczych przez procedurę wielokrotnej transfekcji sekwencji regionu przylegania do macierzy
PL10178871T PL2292754T3 (pl) 2003-10-24 2004-10-22 Wysokowydajny transfer genu i ekspresja w komórkach ssaczych przez procedurę wielokrotnej transfekcji sekwencji regionu przylegania do macierzy
PL10178866T PL2287302T3 (pl) 2003-10-24 2004-10-22 Wysokowydajny transfer genu i ekspresja w komórkach ssaczych przez procedurę wielokrotnej transfekcji sekwencji regionu przylegania do regionu
PL10178873T PL2298898T3 (pl) 2003-10-24 2004-10-22 Wysokowydajny transfer genu i ekspresja w komórkach ssaczych przez procedurę wielokrotnej transfekcji sekwencji regionu przylegania do macierzy

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PL08153753T PL1959011T3 (pl) 2003-10-24 2004-10-22 Wysokowydajny transfer genu i ekspresja w komórkach ssaczych przez procedurę wielokrotnej transfekcji sekwencji regionu przylegania do macierzy

Family Applications After (2)

Application Number Title Priority Date Filing Date
PL10178866T PL2287302T3 (pl) 2003-10-24 2004-10-22 Wysokowydajny transfer genu i ekspresja w komórkach ssaczych przez procedurę wielokrotnej transfekcji sekwencji regionu przylegania do regionu
PL10178873T PL2298898T3 (pl) 2003-10-24 2004-10-22 Wysokowydajny transfer genu i ekspresja w komórkach ssaczych przez procedurę wielokrotnej transfekcji sekwencji regionu przylegania do macierzy

Country Status (16)

Country Link
US (3) US8252917B2 (xx)
EP (5) EP2287302B1 (xx)
JP (1) JP5396653B2 (xx)
KR (4) KR101229989B1 (xx)
CN (1) CN1863913B (xx)
AT (1) ATE551420T1 (xx)
AU (4) AU2004284220B2 (xx)
CA (1) CA2535836C (xx)
HK (4) HK1149777A1 (xx)
IL (2) IL175142A0 (xx)
IN (2) IN2014DN02483A (xx)
PL (4) PL1959011T3 (xx)
RU (1) RU2375450C2 (xx)
SG (1) SG147468A1 (xx)
WO (1) WO2005040377A2 (xx)
ZA (1) ZA200604032B (xx)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004056986A2 (en) 2002-12-20 2004-07-08 Chromagenics B.V. Means and methods for producing a protein through chromatin openers that are capable of rendering chromatin more accessible to transcription factors
IN2014DN02483A (xx) 2003-10-24 2015-05-15 Selexis Sa
DE102004043207C5 (de) * 2004-09-03 2010-09-09 Südzucker AG Mannheim/Ochsenfurt Wurzel- und xylemparenchymspezifischer Promotor
US8999667B2 (en) 2004-11-08 2015-04-07 Chromagenics B.V. Selection of host cells expressing protein at high levels
US20060195935A1 (en) 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels
MX2007004595A (es) 2004-11-08 2007-08-15 Chromagenics Bv Seleccion de celulas hospederas que expresan proteinas en altas concentraciones.
US8039230B2 (en) 2004-11-08 2011-10-18 Chromagenics B.V. Selection of host cells expressing protein at high levels
US7968700B2 (en) 2006-03-20 2011-06-28 Chromagenics B.V. Expression augmenting DNA fragments, use thereof, and methods for finding thereof
KR20090053893A (ko) * 2006-08-23 2009-05-28 셀렉시스 에스. 에이. 전사를 증가시키기 위한 기질부착부위(mars) 및 그의 용도
EP1975228A1 (en) 2007-03-28 2008-10-01 Fachhochschule Mannheim Polynucleotides for enhancing expression of a polynucleotide of interest
DK2222710T3 (en) 2007-12-24 2016-10-03 Id Biomedical Corp Quebec Recombinant RSV antigens.
CN101580842B (zh) * 2008-05-15 2011-02-09 中国农业科学院生物技术研究所 一种增强基因表达的dna分子及其应用
US9181341B2 (en) 2009-01-19 2015-11-10 Innate Pharma Anti-KIR3D antibodies
PE20121541A1 (es) 2009-06-24 2012-12-21 Glaxosmithkline Biolog Sa Antigenos de virus de sincicio respiratorio recombinantes
WO2010149743A2 (en) 2009-06-24 2010-12-29 Id Biomedical Corporation Of Quebec Vaccine
EP4218799A1 (en) 2009-07-15 2023-08-02 GlaxoSmithKline Biologicals S.A. Rsv f protein compositions and methods for making same
CA2774470A1 (en) 2009-09-18 2011-03-24 Selexis S.A. Products and methods for enhanced transgene expression and processing
WO2011070405A1 (en) * 2009-12-07 2011-06-16 Avesthagen Limited An expression vector carrying scaffold/matrix attachment region(s)
WO2012019630A1 (en) 2010-08-13 2012-02-16 Curevac Gmbh Nucleic acid comprising or coding for a histone stem-loop and a poly(a) sequence or a polyadenylation signal for increasing the expression of an encoded protein
WO2012095514A1 (en) 2011-01-14 2012-07-19 Vivalis Recombinant protein production system
CN103620034B (zh) 2011-04-13 2016-12-14 加拿大国家研究委员会 具有来自IFNα2的SAR元件的表达系统
WO2013120499A1 (en) 2012-02-15 2013-08-22 Curevac Gmbh Nucleic acid comprising or coding for a histone stem-loop and a poly (a) sequence or a polyadenylation signal for increasing the expression of an encoded pathogenic antigen
WO2013120498A1 (en) 2012-02-15 2013-08-22 Curevac Gmbh Nucleic acid comprising or coding for a histone stem-loop and a poly(a) sequence or a polyadenylation signal for increasing the expression of an encoded allergenic antigen or an autoimmune self-antigen
WO2013120500A1 (en) 2012-02-15 2013-08-22 Curevac Gmbh Nucleic acid comprising or coding for a histone stem-loop and a poly(a) sequence or a polyadenylation signal for increasing the expression of an encoded tumour antigen
WO2013120497A1 (en) 2012-02-15 2013-08-22 Curevac Gmbh Nucleic acid comprising or coding for a histone stem-loop and a poly(a) sequence or a polyadenylation signal for increasing the expression of an encoded therapeutic protein
CN104284979B (zh) 2012-03-27 2018-06-08 库瑞瓦格股份公司 用于提高的蛋白或肽表达的人工核酸分子
SG10201803758XA (en) 2013-02-01 2018-06-28 Selexis Sa Enhanced Transgene Expression and Processing
CN103667440A (zh) * 2013-09-05 2014-03-26 谢小冬 Gata4基因snp位点的用途
WO2015060935A1 (en) 2013-10-22 2015-04-30 Lubris, Llc Control of rheological properties of mixed hyaluronate/lubricin solutions
CN109576269B (zh) * 2014-04-01 2020-08-28 三生国健药业(上海)股份有限公司 一种含mar核心片段的动物细胞表达载体
CN104974933B (zh) * 2014-04-04 2017-08-15 上海泰因生物技术有限公司 一种大规模连续多次悬浮瞬转表达重组蛋白的装置和方法
WO2017202776A1 (en) * 2016-05-23 2017-11-30 Luxembourg Institute Of Health (Lih) Multifunctional heteromultimeric constructs.
WO2020084528A1 (en) 2018-10-24 2020-04-30 Selexis Sa Expression systems, recombinant cells and uses thereof
CN113383081A (zh) * 2018-12-07 2021-09-10 赛尔希恩公司 基质附着区和促进基因表达的用途
BR112022016481A2 (pt) 2020-02-24 2022-10-25 Novartis Ag Purificação de polipeptídeos recombinantemente produzidos

Family Cites Families (92)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1077994A (en) * 1963-04-18 1967-08-02 Kobe Steel Ltd Process for producing cold-forged products from tempered steel wire
DE2605560C3 (de) 1976-02-12 1979-08-30 Siemens Ag, 1000 Berlin Und 8000 Muenchen Aufschlußverfahren für Biomaterialien
EP0113551B1 (en) 1982-12-23 1988-04-27 Britax Weathershields Limited Vehicle sliding roof
DE122007000007I1 (de) 1986-04-09 2007-05-16 Genzyme Corp Genetisch transformierte Tiere, die ein gewünschtes Protein in Milch absondern
US4873316A (en) 1987-06-23 1989-10-10 Biogen, Inc. Isolation of exogenous recombinant proteins from the milk of transgenic mammals
GB8925590D0 (en) 1989-11-13 1990-01-04 Central Blood Lab Authority Monoclonal antibodies
US6063630A (en) 1991-11-05 2000-05-16 Transkaryotic Therapies, Inc. Targeted introduction of DNA into primary or secondary cells and their use for gene therapy
US5968502A (en) 1991-11-05 1999-10-19 Transkaryotic Therapies, Inc. Protein production and protein delivery
CA2147006C (en) 1992-10-05 2004-11-30 William F. Thompson Method for increasing expression and reducing expression variability of foreign genes in plant cells
US5610053A (en) 1993-04-07 1997-03-11 The United States Of America As Represented By The Department Of Health And Human Services DNA sequence which acts as a chromatin insulator element to protect expressed genes from cis-acting regulatory sequences in mammalian cells
US5888981A (en) 1993-06-14 1999-03-30 Basf Aktiengesellschaft Methods for regulating gene expression
US5464758A (en) 1993-06-14 1995-11-07 Gossen; Manfred Tight control of gene expression in eucaryotic cells by tetracycline-responsive promoters
US5907078A (en) 1994-12-09 1999-05-25 Greenberg; Norman M. Transgenic mouse model for prostate cancer
US5705364A (en) 1995-06-06 1998-01-06 Genentech, Inc. Mammalian cell culture process
DE19541450C2 (de) 1995-11-07 1997-10-02 Gsf Forschungszentrum Umwelt Genkonstrukt und dessen Verwendung
US6596514B2 (en) 1996-01-11 2003-07-22 Immunex Corporation Expression augmenting sequence elements (EASE) for eukaryotic expression systems
US5773695A (en) 1996-01-26 1998-06-30 North Carolina State University Plant nuclear scaffold attachment region and method for increasing gene expression in transgenic cells
WO1997032018A2 (en) 1996-02-29 1997-09-04 Immusol, Inc. Hepatitis c virus ribozymes
IL127215A0 (en) 1996-06-06 1999-09-22 Novartis Ag Vectors comprising sar elements
EP1471144B1 (en) 1996-12-02 2010-06-23 Genaera Corporation Biological variability of asthma associated factor AAF2 (IL-9 receptor) useful in treating and diagnosing atopic allergies including asthma and related disorders
US6245974B1 (en) * 1997-08-06 2001-06-12 North Carolina State University Matrix attachment regions
US6897066B1 (en) 1997-09-26 2005-05-24 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes
WO1999022773A2 (en) 1997-11-05 1999-05-14 Baylor College Of Medicine Sequences for targeting metastatic cells
US6635806B1 (en) 1998-05-14 2003-10-21 Dekalb Genetics Corporation Methods and compositions for expression of transgenes in plants
US6004284A (en) 1998-06-04 1999-12-21 Alcon Laboratories, Inc. Surgical handpiece
WO2000005393A2 (en) 1998-07-21 2000-02-03 Cobra Therapeutics Limited A polynucleotide comprising a ubiquitous chromatin opening element (ucoe)
US6338066B1 (en) 1998-09-25 2002-01-08 International Business Machines Corporation Surfaid predictor: web-based system for predicting surfer behavior
WO2000020950A1 (en) 1998-10-07 2000-04-13 Adobe Systems, Inc. Distributing access to a data item
DE19848017A1 (de) 1998-10-17 2000-04-20 Multigene Biotech Gmbh Episomal replizierender Vektor zur Expression eines Transgens in Säugerzellen
JP4544748B2 (ja) * 1998-12-01 2010-09-15 ダウ・アグロサイエンス・エル・エル・シー 植物細胞内に導入された遺伝子の発現を増加するための人工マトリックス付着領域
US6225113B1 (en) 1998-12-04 2001-05-01 Genvec, Inc. Use of trans-activation and cis-activation to modulate the persistence of expression of a transgene in an at least E4-deficient adenovirus
US6207879B1 (en) 1999-05-14 2001-03-27 Dekalb Genetics Corporation Maize RS81 promoter and methods for use thereof
US6232526B1 (en) 1999-05-14 2001-05-15 Dekalb Genetics Corp. Maize A3 promoter and methods for use thereof
US6194636B1 (en) 1999-05-14 2001-02-27 Dekalb Genetics Corp. Maize RS324 promoter and methods for use thereof
US6429357B1 (en) 1999-05-14 2002-08-06 Dekalb Genetics Corp. Rice actin 2 promoter and intron and methods for use thereof
AU4940700A (en) 1999-05-28 2000-12-18 Sangamo Biosciences, Inc. Gene switches
US20050129669A1 (en) 1999-06-21 2005-06-16 Transkaryotic Therapies, Inc., A Massachusetts Corporation DNA construct for effecting homologous recombination and uses thereof
US6972349B1 (en) 1999-11-12 2005-12-06 University Of South Carolina Control of post-transcriptional gene silencing in plants
JP3507858B2 (ja) 1999-11-19 2004-03-15 奈良先端科学技術大学院大学長 植物において外来遺伝子の発現を安定化させるdna断片、外来遺伝子を植物において安定に発現させる遺伝子導入方法、外来遺伝子を安定に発現させた形質転換植物
WO2001040488A1 (en) 1999-11-30 2001-06-07 Novartis Ag Increased transgene expression in retroviral vectors having scaffold attachment region
US6864085B2 (en) 1999-12-14 2005-03-08 Novartis Ag Bovine immunodeficiency virus (BIV) based vectors
US20020127199A1 (en) 2000-02-03 2002-09-12 Tang Y. Tom Novel nucleic acids and polypeptides
US6821775B1 (en) 2000-02-11 2004-11-23 Genvec, Inc. Viral vector encoding pigment epithelium-derived factor
DE10006886A1 (de) 2000-02-16 2001-08-23 Hepavec Ag Fuer Gentherapie Nichthumaner helferabhängiger Virusvektor
US6569681B1 (en) 2000-03-14 2003-05-27 Transkaryotic Therapies, Inc. Methods of improving homologous recombination
US6747189B1 (en) 2000-03-21 2004-06-08 Dekalb Genetics Corporation Maize glycine rich protein promoter compositions and methods for use thereof
US6730826B2 (en) 2000-03-22 2004-05-04 University Of Kentucky Research Foundation Plant trichome gland specific promoter sequence
EP1294406A2 (en) 2000-06-26 2003-03-26 Universite De Geneve Modulation of chromosome function by chromatin remodeling agents
US20030157715A1 (en) 2000-06-26 2003-08-21 Ulrich Laemmli Modulation of chromosome function by chromatin remodeling agents
US8030537B1 (en) 2000-07-27 2011-10-04 Apogene Gmbh & Co. Kg Somatic cloning gene transfer for the production of recombinant proteins, cells and organs
KR100408844B1 (ko) 2000-07-29 2003-12-06 한국산업기술평가원 동물세포 발현벡터
WO2002018607A2 (en) 2000-08-30 2002-03-07 North Carolina State University Transgenic plants containing molecular decoys that alter protein content therein
WO2002020811A2 (en) 2000-09-09 2002-03-14 Basf Plant Science Gmbh Modified tet-inducible system for regulation of gene expression in plants
GB0022995D0 (en) 2000-09-20 2000-11-01 Cobra Therapeutics Ltd Polynucleotide
US6919204B2 (en) 2000-09-29 2005-07-19 Sangamo Biosciences, Inc. Modulation of gene expression using localization domains
AU4155602A (en) 2000-11-01 2002-06-18 Elusys Therapeutics Inc Method of producing biospecific molecules by protein trans-splicing
DE10061150A1 (de) 2000-12-08 2002-06-13 Icon Genetics Ag Verfahren und Vektoren zur Erzeugung von transgenen Pflanzen
US7259010B2 (en) 2000-12-15 2007-08-21 Pangen Biotech Inc. Expression vector for animal cell containing nuclear matrix attachment region of interferon beta
US7151204B2 (en) 2001-01-09 2006-12-19 Monsanto Technology Llc Maize chloroplast aldolase promoter compositions and methods for use thereof
EP1395669B1 (en) 2001-01-26 2009-07-22 Selexis S.A. Matrix attachment regions and methods for use thereof
DE10109780A1 (de) 2001-02-28 2002-09-05 Cardion Ag Episomale Vektoren enthaltend Matrix-Anheftungsregionen zur zellspezifischen Genexpression
CA2441006A1 (en) 2001-03-08 2002-09-19 Hyseq, Inc. Methods and materials relating to fibulin-like polypeptides and polynucleotides
JP4817514B2 (ja) 2001-03-09 2011-11-16 協和発酵キリン株式会社 新規動物細胞用ベクターおよびその使用
AU2002252370A1 (en) 2001-03-12 2002-09-24 Irm, Llc. Genomics-driven high speed cellular assays, development thereof, and collections of cellular reporters
EP1967590A3 (en) 2001-03-13 2008-09-17 Novartis AG Lentiviral packaging constructs
EP1478751A4 (en) 2001-03-30 2005-10-19 Avigenics Inc LYSOZYM PROMOTER IN BIRDS
DE10129010A1 (de) 2001-06-13 2002-12-19 Icon Genetics Ag Verfahren und Vektoren zur Erzeugung von transgenen Pflanzen
US20030032597A1 (en) 2001-07-31 2003-02-13 Sebestyen Magdolna G. Targeting nucleic acids to a cellular nucleus
KR100464962B1 (ko) * 2001-09-14 2005-01-05 삼화강봉주식회사 냉간압조 특성이 우수한 조질 강선
US7122715B2 (en) 2001-09-20 2006-10-17 Monsanto Technology Llc In vitro method to create circular molecules for use in transformation
WO2003024199A2 (en) 2001-09-21 2003-03-27 Avigenics, Inc. Production of transgenic avians using sperm-mediated transfection
US7138278B2 (en) 2001-11-20 2006-11-21 Monsanto Technology, L.L.C. Maize cytoplasmic glutamine synthetase promoter compositions and methods for use thereof
FR2832423B1 (fr) 2001-11-22 2004-10-08 Vivalis Systeme d'expression de proteines exogenes dans un systeme aviaire
US6875588B2 (en) 2001-11-30 2005-04-05 Avigenics, Inc. Ovomucoid promoter and methods of use
CA2427190A1 (en) 2002-04-30 2003-10-30 Alberta Research Council Inc. Production of recombinant epidermal growth factor in plants
US20030224477A1 (en) 2002-05-31 2003-12-04 Heartlein Michael W. Optimized promoter constructs
CA2488924A1 (en) 2002-06-18 2003-12-24 Zymogenetics, Inc. Hybrid vector having a cytomegalovirus enhancer and myeloproliferative sarcoma virus promoter
US20040016015A1 (en) 2002-07-17 2004-01-22 Nguyen Thanh-Tuyen T. High efficiency germline transformation system
US6933136B2 (en) 2002-09-20 2005-08-23 Novo Nordisk A/S Method for making recombinant proteins
CN1263860C (zh) 2002-09-30 2006-07-12 华南农业大学 多基因载体的构建方法与应用
WO2004053106A2 (en) 2002-12-05 2004-06-24 Regulome Corporation Profiled regulatory sites useful for gene control
AU2003283174A1 (en) 2002-12-11 2004-06-30 Cytos Biotechnology Ag Method for protein production
AU2002953381A0 (en) 2002-12-18 2003-01-09 Diatech Pty Ltd In vivo affinity maturation scheme
EP1445320A1 (en) 2003-02-05 2004-08-11 ARTEMIS Pharmaceuticals GmbH Automated gene-targeting using non-toxic detectable markers
EP1623035A4 (en) 2003-04-15 2006-08-30 Univ Georgia Res Found AVIAN TRANSGENESE USING A REGION OF THE CHICKEN OVALBUMIN GENE
WO2004106375A1 (en) 2003-05-30 2004-12-09 Merus Biopharmaceuticals B.V. I.O. Fab library for the preparation of anti vegf and anti rabies virus fabs
EP1644508A1 (en) 2003-07-11 2006-04-12 Cytos Biotechnology AG Gene expression system
RU2373285C2 (ru) 2003-08-27 2009-11-20 Орф Лифтаэкни Хф. Способ увеличения накопления гетерологичных полипептидов в семенах растений посредством направленной супрессии эндогенных запасных белков
ES2354160T3 (es) 2003-10-21 2011-03-10 Merck Serono Sa Secuencia de adn mínima que actúa como un aislante de cromatina, y su uso en la expresión de proteínas.
IN2014DN02483A (xx) * 2003-10-24 2015-05-15 Selexis Sa
KR20090053893A (ko) 2006-08-23 2009-05-28 셀렉시스 에스. 에이. 전사를 증가시키기 위한 기질부착부위(mars) 및 그의 용도
WO2008073303A2 (en) * 2006-12-07 2008-06-19 Switchgear Genomics Transcriptional regulatory elements of biological pathways, tools, and methods

Also Published As

Publication number Publication date
EP2292754B1 (en) 2012-12-26
KR101230838B1 (ko) 2013-02-06
KR20060100372A (ko) 2006-09-20
IL198863A (en) 2011-09-27
EP2287302A1 (en) 2011-02-23
EP1959011B1 (en) 2012-03-28
JP5396653B2 (ja) 2014-01-22
AU2010236072B2 (en) 2011-09-01
CA2535836C (en) 2014-02-04
EP1959011A2 (en) 2008-08-20
JP2007508831A (ja) 2007-04-12
IL175142A0 (en) 2006-09-05
RU2006117757A (ru) 2007-11-27
PL2298898T3 (pl) 2013-05-31
AU2004284220A1 (en) 2005-05-06
EP2287302B1 (en) 2012-12-26
AU2004284220B2 (en) 2010-10-14
SG147468A1 (en) 2008-11-28
KR20110112882A (ko) 2011-10-13
EP2298898B1 (en) 2012-12-26
KR101222025B1 (ko) 2013-01-15
AU2010236091A1 (en) 2010-11-25
WO2005040377A3 (en) 2005-09-15
CN1863913A (zh) 2006-11-15
EP1675952A2 (en) 2006-07-05
HK1151824A1 (en) 2012-02-10
US20130143264A1 (en) 2013-06-06
HK1149777A1 (en) 2011-10-14
US8252917B2 (en) 2012-08-28
PL1959011T3 (pl) 2012-07-31
RU2375450C2 (ru) 2009-12-10
IN2014DN02484A (xx) 2015-05-15
AU2010236091B2 (en) 2011-07-07
WO2005040377A2 (en) 2005-05-06
US9879297B2 (en) 2018-01-30
PL2287302T3 (pl) 2013-05-31
CA2535836A1 (en) 2005-05-06
EP2292754A1 (en) 2011-03-09
KR101269656B1 (ko) 2013-05-30
US10669562B2 (en) 2020-06-02
KR20110112884A (ko) 2011-10-13
KR20110112883A (ko) 2011-10-13
ATE551420T1 (de) 2012-04-15
AU2010236093B2 (en) 2011-10-13
EP2298898A1 (en) 2011-03-23
US20070178469A1 (en) 2007-08-02
AU2010236072A1 (en) 2010-11-18
AU2010236093A1 (en) 2010-12-02
HK1151825A1 (en) 2012-02-10
IN2014DN02483A (xx) 2015-05-15
KR101229989B1 (ko) 2013-02-06
HK1120549A1 (en) 2009-04-03
CN1863913B (zh) 2011-01-12
US20180187229A1 (en) 2018-07-05
EP1959011A3 (en) 2008-08-27
ZA200604032B (en) 2008-05-28

Similar Documents

Publication Publication Date Title
HK1151825A1 (en) High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of matrix attachment region sequences
ATE300613T1 (de) Menschliche, für zyklische nukleotide spezifische phosphodiesterase
DK1622921T3 (da) Varianter af beta-glucosidaser
BR0003943A (pt) Sequências de nucleotìdeo que codificam para o gene thre e processo para a produção enzimática de l-treonina usando bactérias corineforma
CY1106978T1 (el) Ενζυμα φωσφοδιεστερασης
CY1106041T1 (el) Καινοφανης συλλεκτινη
ZA200102385B (en) Nucleotide sequences coding for the dapC gene and process for the production of L-lysine.
GB0020331D0 (en) Enzyme
TR200003272T2 (tr) Protein üretimi verilmesi için G-CSF geninin genomik sekanslar.
DE602005027824D1 (de) Zur beschleunigung der genexpressionbei einer mässig niedrigen temperatur fähige sequenz
ATE346153T1 (de) Endogene retrovirale sequenzen, verwandten mit auto-immune krankheiten oder mit schwangerschaftsstörungen
MXPA02012557A (es) Composiciones y metodos para la modificacion de la expresion del gen.
NO20011995D0 (no) Fremgangsmåte for å produsere koenzymet Q10
SG128684A1 (en) Novel sequence and utilization thereof
EP2053127A3 (en) Isolated polynucleotides having a reduced or an increased content of epigenetic control motifs and uses thereof
WO2004042066A3 (en) NtSM GENE
ATE403730T1 (de) Gene, die fuer kohlenstoffmetabolismus- und energieproduktions-proteine codieren
TR200003271T2 (tr) Protein üretimi ve verilmesi için genomik sekanslar.
ATE339508T1 (de) Mites-ähnliches element und an trankriptionsaktivierung beteiligtes element
ATE296883T1 (de) Mn-gen und -protein
TH48289A3 (th) ลำดับลักษณะทางพันธุกรรม สำหรับการผลิตโปรตีนและการปล่อย