PL205966B1 - Ciągła amniocytarna linia komórkowa, sposób jej wytwarzania, zastosowanie amniocytów, zastosowanie cząsteczki kwasu nukleinowego, zastosowanie ciągłej amniocytarnej linii komórkowej i sposób wytwarzania wektorów przenoszących geny i/lub mutantów adenowirusowych - Google Patents
Ciągła amniocytarna linia komórkowa, sposób jej wytwarzania, zastosowanie amniocytów, zastosowanie cząsteczki kwasu nukleinowego, zastosowanie ciągłej amniocytarnej linii komórkowej i sposób wytwarzania wektorów przenoszących geny i/lub mutantów adenowirusowychInfo
- Publication number
- PL205966B1 PL205966B1 PL357495A PL35749500A PL205966B1 PL 205966 B1 PL205966 B1 PL 205966B1 PL 357495 A PL357495 A PL 357495A PL 35749500 A PL35749500 A PL 35749500A PL 205966 B1 PL205966 B1 PL 205966B1
- Authority
- PL
- Poland
- Prior art keywords
- adenoviral
- vectors
- cell line
- cells
- gene
- Prior art date
Links
- 239000013598 vector Substances 0.000 title claims description 250
- 108090000623 proteins and genes Proteins 0.000 title claims description 165
- 238000004519 manufacturing process Methods 0.000 title claims description 69
- 210000001776 amniocyte Anatomy 0.000 title claims description 56
- 238000012546 transfer Methods 0.000 title claims description 36
- 230000014509 gene expression Effects 0.000 claims abstract description 45
- 241000701161 unidentified adenovirus Species 0.000 claims abstract description 42
- 210000004027 cell Anatomy 0.000 claims description 369
- 101150075174 E1B gene Proteins 0.000 claims description 108
- 108020004414 DNA Proteins 0.000 claims description 75
- 150000007523 nucleic acids Chemical class 0.000 claims description 54
- 241001135569 Human adenovirus 5 Species 0.000 claims description 51
- 238000000034 method Methods 0.000 claims description 41
- 241000702421 Dependoparvovirus Species 0.000 claims description 32
- 102000039446 nucleic acids Human genes 0.000 claims description 29
- 108020004707 nucleic acids Proteins 0.000 claims description 29
- 238000001890 transfection Methods 0.000 claims description 27
- 102000011755 Phosphoglycerate Kinase Human genes 0.000 claims description 22
- 101001099217 Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) Triosephosphate isomerase Proteins 0.000 claims description 22
- 230000001177 retroviral effect Effects 0.000 claims description 17
- 108010051219 Cre recombinase Proteins 0.000 claims description 14
- 230000035772 mutation Effects 0.000 claims description 9
- 230000010415 tropism Effects 0.000 claims description 9
- 210000005260 human cell Anatomy 0.000 claims description 6
- 239000002773 nucleotide Substances 0.000 claims description 6
- 125000003729 nucleotide group Chemical group 0.000 claims description 6
- 108010024878 Adenovirus E1A Proteins Proteins 0.000 claims description 5
- 108010087905 Adenovirus E1B Proteins Proteins 0.000 claims description 5
- 239000013604 expression vector Substances 0.000 claims description 4
- 230000008569 process Effects 0.000 claims description 4
- 108010057856 Adenovirus E2 Proteins Proteins 0.000 claims description 3
- 239000013612 plasmid Substances 0.000 description 50
- 230000003612 virological effect Effects 0.000 description 43
- 241000700605 Viruses Species 0.000 description 39
- 230000006870 function Effects 0.000 description 34
- 239000000047 product Substances 0.000 description 34
- 208000015181 infectious disease Diseases 0.000 description 31
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 26
- 102000004169 proteins and genes Human genes 0.000 description 26
- 235000018102 proteins Nutrition 0.000 description 25
- 238000012217 deletion Methods 0.000 description 22
- 230000037430 deletion Effects 0.000 description 22
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 18
- 238000004806 packaging method and process Methods 0.000 description 18
- 230000010076 replication Effects 0.000 description 18
- 210000000234 capsid Anatomy 0.000 description 17
- 101710145505 Fiber protein Proteins 0.000 description 16
- 201000005569 Gout Diseases 0.000 description 16
- 239000012634 fragment Substances 0.000 description 16
- 230000009466 transformation Effects 0.000 description 16
- 239000013607 AAV vector Substances 0.000 description 15
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 13
- 238000004113 cell culture Methods 0.000 description 13
- 238000002474 experimental method Methods 0.000 description 13
- 239000002609 medium Substances 0.000 description 13
- 230000012010 growth Effects 0.000 description 12
- 241000702191 Escherichia virus P1 Species 0.000 description 11
- 206010028980 Neoplasm Diseases 0.000 description 11
- 201000011510 cancer Diseases 0.000 description 11
- 238000010367 cloning Methods 0.000 description 10
- 230000010354 integration Effects 0.000 description 10
- 210000001519 tissue Anatomy 0.000 description 10
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 9
- 230000015572 biosynthetic process Effects 0.000 description 9
- 230000001413 cellular effect Effects 0.000 description 9
- 210000003754 fetus Anatomy 0.000 description 9
- 230000008488 polyadenylation Effects 0.000 description 9
- 230000001225 therapeutic effect Effects 0.000 description 9
- 230000001131 transforming effect Effects 0.000 description 9
- 239000013603 viral vector Substances 0.000 description 9
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 8
- 210000004381 amniotic fluid Anatomy 0.000 description 8
- 108010005774 beta-Galactosidase Proteins 0.000 description 8
- 239000012894 fetal calf serum Substances 0.000 description 8
- 239000003446 ligand Substances 0.000 description 8
- 239000002245 particle Substances 0.000 description 8
- 102100038313 Transcription factor E2-alpha Human genes 0.000 description 7
- 230000027455 binding Effects 0.000 description 7
- 239000000835 fiber Substances 0.000 description 7
- 210000003128 head Anatomy 0.000 description 7
- 230000004048 modification Effects 0.000 description 7
- 238000012986 modification Methods 0.000 description 7
- CXURGFRDGROIKG-UHFFFAOYSA-N 3,3-bis(chloromethyl)oxetane Chemical compound ClCC1(CCl)COC1 CXURGFRDGROIKG-UHFFFAOYSA-N 0.000 description 6
- WQZGKKKJIJFFOK-FPRJBGLDSA-N beta-D-galactose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-FPRJBGLDSA-N 0.000 description 6
- 238000001962 electrophoresis Methods 0.000 description 6
- 238000000338 in vitro Methods 0.000 description 6
- 230000003993 interaction Effects 0.000 description 6
- 108020003175 receptors Proteins 0.000 description 6
- 102000005962 receptors Human genes 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 238000010186 staining Methods 0.000 description 6
- 238000013518 transcription Methods 0.000 description 6
- 230000035897 transcription Effects 0.000 description 6
- 241000283690 Bos taurus Species 0.000 description 5
- 241000701022 Cytomegalovirus Species 0.000 description 5
- 229930182555 Penicillin Natural products 0.000 description 5
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 5
- 108700005077 Viral Genes Proteins 0.000 description 5
- 238000001574 biopsy Methods 0.000 description 5
- 230000000295 complement effect Effects 0.000 description 5
- 235000015097 nutrients Nutrition 0.000 description 5
- 210000000056 organ Anatomy 0.000 description 5
- 238000012856 packing Methods 0.000 description 5
- 229940049954 penicillin Drugs 0.000 description 5
- 108020001580 protein domains Proteins 0.000 description 5
- 230000003362 replicative effect Effects 0.000 description 5
- 241000894007 species Species 0.000 description 5
- QTENRWWVYAAPBI-YZTFXSNBSA-N Streptomycin sulfate Chemical compound OS(O)(=O)=O.OS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@H]1[C@H](N=C(N)N)[C@@H](O)[C@H](N=C(N)N)[C@@H](O)[C@@H]1O.CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@H]1[C@H](N=C(N)N)[C@@H](O)[C@H](N=C(N)N)[C@@H](O)[C@@H]1O QTENRWWVYAAPBI-YZTFXSNBSA-N 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 210000000349 chromosome Anatomy 0.000 description 4
- 230000000120 cytopathologic effect Effects 0.000 description 4
- 238000012869 ethanol precipitation Methods 0.000 description 4
- 239000013613 expression plasmid Substances 0.000 description 4
- 238000000605 extraction Methods 0.000 description 4
- 210000001508 eye Anatomy 0.000 description 4
- 238000001415 gene therapy Methods 0.000 description 4
- 230000006801 homologous recombination Effects 0.000 description 4
- 238000002744 homologous recombination Methods 0.000 description 4
- 238000001727 in vivo Methods 0.000 description 4
- 230000002458 infectious effect Effects 0.000 description 4
- 238000003780 insertion Methods 0.000 description 4
- 230000037431 insertion Effects 0.000 description 4
- 102000006495 integrins Human genes 0.000 description 4
- 108010044426 integrins Proteins 0.000 description 4
- 230000002101 lytic effect Effects 0.000 description 4
- 239000003550 marker Substances 0.000 description 4
- 230000035935 pregnancy Effects 0.000 description 4
- 108090000765 processed proteins & peptides Proteins 0.000 description 4
- 230000002207 retinal effect Effects 0.000 description 4
- 238000003786 synthesis reaction Methods 0.000 description 4
- 238000010361 transduction Methods 0.000 description 4
- 230000026683 transduction Effects 0.000 description 4
- 210000004881 tumor cell Anatomy 0.000 description 4
- 241001529453 unidentified herpesvirus Species 0.000 description 4
- 208000003322 Coinfection Diseases 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 108010014594 Heterogeneous Nuclear Ribonucleoprotein A1 Proteins 0.000 description 3
- 101710094396 Hexon protein Proteins 0.000 description 3
- 101710155188 Hexon-interlacing protein Proteins 0.000 description 3
- 108091034117 Oligonucleotide Proteins 0.000 description 3
- 238000012408 PCR amplification Methods 0.000 description 3
- 108020005067 RNA Splice Sites Proteins 0.000 description 3
- 241000700159 Rattus Species 0.000 description 3
- 238000002105 Southern blotting Methods 0.000 description 3
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 3
- 230000000843 anti-fungal effect Effects 0.000 description 3
- 229940121375 antifungal agent Drugs 0.000 description 3
- 230000006907 apoptotic process Effects 0.000 description 3
- 230000003115 biocidal effect Effects 0.000 description 3
- 238000010370 cell cloning Methods 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 239000002299 complementary DNA Substances 0.000 description 3
- 230000030944 contact inhibition Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 210000002919 epithelial cell Anatomy 0.000 description 3
- 230000001605 fetal effect Effects 0.000 description 3
- 230000008014 freezing Effects 0.000 description 3
- 238000007710 freezing Methods 0.000 description 3
- 210000002510 keratinocyte Anatomy 0.000 description 3
- 210000003292 kidney cell Anatomy 0.000 description 3
- 108020004999 messenger RNA Proteins 0.000 description 3
- 238000003793 prenatal diagnosis Methods 0.000 description 3
- 238000010257 thawing Methods 0.000 description 3
- 230000017105 transposition Effects 0.000 description 3
- 238000001262 western blot Methods 0.000 description 3
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 2
- 108020005544 Antisense RNA Proteins 0.000 description 2
- IYMAXBFPHPZYIK-BQBZGAKWSA-N Arg-Gly-Asp Chemical compound NC(N)=NCCC[C@H](N)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(O)=O IYMAXBFPHPZYIK-BQBZGAKWSA-N 0.000 description 2
- 108090000565 Capsid Proteins Proteins 0.000 description 2
- 201000009030 Carcinoma Diseases 0.000 description 2
- 102100023321 Ceruloplasmin Human genes 0.000 description 2
- 241000709687 Coxsackievirus Species 0.000 description 2
- 102000053602 DNA Human genes 0.000 description 2
- 230000004543 DNA replication Effects 0.000 description 2
- 108010069091 Dystrophin Proteins 0.000 description 2
- 101150029662 E1 gene Proteins 0.000 description 2
- 101150066038 E4 gene Proteins 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 108010054218 Factor VIII Proteins 0.000 description 2
- 102000003974 Fibroblast growth factor 2 Human genes 0.000 description 2
- 108090000379 Fibroblast growth factor 2 Proteins 0.000 description 2
- 241000282412 Homo Species 0.000 description 2
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 2
- 241000598171 Human adenovirus sp. Species 0.000 description 2
- 102100021244 Integral membrane protein GPR180 Human genes 0.000 description 2
- 108010059343 MM Form Creatine Kinase Proteins 0.000 description 2
- 241001529936 Murinae Species 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 2
- 102000007981 Ornithine carbamoyltransferase Human genes 0.000 description 2
- 101710198224 Ornithine carbamoyltransferase, mitochondrial Proteins 0.000 description 2
- 108010002747 Pfu DNA polymerase Proteins 0.000 description 2
- 241000288906 Primates Species 0.000 description 2
- 108010091086 Recombinases Proteins 0.000 description 2
- 102000018120 Recombinases Human genes 0.000 description 2
- 108700008625 Reporter Genes Proteins 0.000 description 2
- 241000283984 Rodentia Species 0.000 description 2
- 206010039491 Sarcoma Diseases 0.000 description 2
- 238000012300 Sequence Analysis Methods 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 101710172711 Structural protein Proteins 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 108010003533 Viral Envelope Proteins Proteins 0.000 description 2
- 108010067390 Viral Proteins Proteins 0.000 description 2
- 108010087302 Viral Structural Proteins Proteins 0.000 description 2
- 206010000210 abortion Diseases 0.000 description 2
- 231100000176 abortion Toxicity 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 150000007513 acids Chemical class 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 108010050122 alpha 1-Antitrypsin Proteins 0.000 description 2
- 230000001093 anti-cancer Effects 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 description 2
- 230000022131 cell cycle Effects 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000001427 coherent effect Effects 0.000 description 2
- 210000000805 cytoplasm Anatomy 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 210000003494 hepatocyte Anatomy 0.000 description 2
- 230000002779 inactivation Effects 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 210000003734 kidney Anatomy 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 210000004779 membrane envelope Anatomy 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000000877 morphologic effect Effects 0.000 description 2
- 210000005157 neural retina Anatomy 0.000 description 2
- 210000004940 nucleus Anatomy 0.000 description 2
- 231100000590 oncogenic Toxicity 0.000 description 2
- 230000002246 oncogenic effect Effects 0.000 description 2
- YBYRMVIVWMBXKQ-UHFFFAOYSA-N phenylmethanesulfonyl fluoride Chemical compound FS(=O)(=O)CC1=CC=CC=C1 YBYRMVIVWMBXKQ-UHFFFAOYSA-N 0.000 description 2
- 230000007505 plaque formation Effects 0.000 description 2
- 230000010837 receptor-mediated endocytosis Effects 0.000 description 2
- 230000006798 recombination Effects 0.000 description 2
- 238000005215 recombination Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 210000001626 skin fibroblast Anatomy 0.000 description 2
- 208000000995 spontaneous abortion Diseases 0.000 description 2
- 230000010473 stable expression Effects 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000003146 transient transfection Methods 0.000 description 2
- 230000032258 transport Effects 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- 241001430294 unidentified retrovirus Species 0.000 description 2
- 210000001635 urinary tract Anatomy 0.000 description 2
- 230000002792 vascular Effects 0.000 description 2
- 206010000234 Abortion spontaneous Diseases 0.000 description 1
- 101100524317 Adeno-associated virus 2 (isolate Srivastava/1982) Rep40 gene Proteins 0.000 description 1
- 101100524319 Adeno-associated virus 2 (isolate Srivastava/1982) Rep52 gene Proteins 0.000 description 1
- 101100524321 Adeno-associated virus 2 (isolate Srivastava/1982) Rep68 gene Proteins 0.000 description 1
- 101100524324 Adeno-associated virus 2 (isolate Srivastava/1982) Rep78 gene Proteins 0.000 description 1
- 206010001258 Adenoviral infections Diseases 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 102100026189 Beta-galactosidase Human genes 0.000 description 1
- 102000015081 Blood Coagulation Factors Human genes 0.000 description 1
- 108010039209 Blood Coagulation Factors Proteins 0.000 description 1
- 101150029409 CFTR gene Proteins 0.000 description 1
- 206010007269 Carcinogenicity Diseases 0.000 description 1
- 108090000994 Catalytic RNA Proteins 0.000 description 1
- 102000053642 Catalytic RNA Human genes 0.000 description 1
- 102000005483 Cell Cycle Proteins Human genes 0.000 description 1
- 108010031896 Cell Cycle Proteins Proteins 0.000 description 1
- 108010079245 Cystic Fibrosis Transmembrane Conductance Regulator Proteins 0.000 description 1
- 201000003883 Cystic fibrosis Diseases 0.000 description 1
- 102000052510 DNA-Binding Proteins Human genes 0.000 description 1
- 108700020911 DNA-Binding Proteins Proteins 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 101100364969 Dictyostelium discoideum scai gene Proteins 0.000 description 1
- 102000001039 Dystrophin Human genes 0.000 description 1
- 101710199711 Early E1A protein Proteins 0.000 description 1
- 108700024394 Exon Proteins 0.000 description 1
- 102000001690 Factor VIII Human genes 0.000 description 1
- 102000004862 Gastrin releasing peptide Human genes 0.000 description 1
- 108090001053 Gastrin releasing peptide Proteins 0.000 description 1
- 241000696272 Gull adenovirus Species 0.000 description 1
- 102100021519 Hemoglobin subunit beta Human genes 0.000 description 1
- 108091005904 Hemoglobin subunit beta Proteins 0.000 description 1
- 241000700721 Hepatitis B virus Species 0.000 description 1
- 241000175212 Herpesvirales Species 0.000 description 1
- 241000709727 Human poliovirus 3 Species 0.000 description 1
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 1
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 1
- 229930182816 L-glutamine Natural products 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- 101710192606 Latent membrane protein 2 Proteins 0.000 description 1
- 241000713666 Lentivirus Species 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- NTIZESTWPVYFNL-UHFFFAOYSA-N Methyl isobutyl ketone Chemical compound CC(C)CC(C)=O NTIZESTWPVYFNL-UHFFFAOYSA-N 0.000 description 1
- 101100364971 Mus musculus Scai gene Proteins 0.000 description 1
- 102000008934 Muscle Proteins Human genes 0.000 description 1
- 108010074084 Muscle Proteins Proteins 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 101150057876 OTC gene Proteins 0.000 description 1
- 108700020796 Oncogene Proteins 0.000 description 1
- 102000043276 Oncogene Human genes 0.000 description 1
- 108700026244 Open Reading Frames Proteins 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 241000282520 Papio Species 0.000 description 1
- 101710173835 Penton protein Proteins 0.000 description 1
- 108010039918 Polylysine Proteins 0.000 description 1
- 101710150114 Protein rep Proteins 0.000 description 1
- 241000125945 Protoparvovirus Species 0.000 description 1
- 108091034057 RNA (poly(A)) Proteins 0.000 description 1
- 230000007022 RNA scission Effects 0.000 description 1
- 101710152114 Replication protein Proteins 0.000 description 1
- 208000016624 Retinal neoplasm Diseases 0.000 description 1
- 108700025701 Retinoblastoma Genes Proteins 0.000 description 1
- 230000018199 S phase Effects 0.000 description 1
- 210000001744 T-lymphocyte Anatomy 0.000 description 1
- 101710109576 Terminal protein Proteins 0.000 description 1
- 241000906446 Theraps Species 0.000 description 1
- 102000006601 Thymidine Kinase Human genes 0.000 description 1
- 108020004440 Thymidine kinase Proteins 0.000 description 1
- 108091023040 Transcription factor Proteins 0.000 description 1
- 102000040945 Transcription factor Human genes 0.000 description 1
- 229920004890 Triton X-100 Polymers 0.000 description 1
- 239000013504 Triton X-100 Substances 0.000 description 1
- 108010078814 Tumor Suppressor Protein p53 Proteins 0.000 description 1
- 241000700618 Vaccinia virus Species 0.000 description 1
- 108020005202 Viral DNA Proteins 0.000 description 1
- 108020000999 Viral RNA Proteins 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 102000015395 alpha 1-Antitrypsin Human genes 0.000 description 1
- 229940024142 alpha 1-antitrypsin Drugs 0.000 description 1
- VREFGVBLTWBCJP-UHFFFAOYSA-N alprazolam Chemical compound C12=CC(Cl)=CC=C2N2C(C)=NN=C2CN=C1C1=CC=CC=C1 VREFGVBLTWBCJP-UHFFFAOYSA-N 0.000 description 1
- 229940024606 amino acid Drugs 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000002669 amniocentesis Methods 0.000 description 1
- 210000001691 amnion Anatomy 0.000 description 1
- 230000007416 antiviral immune response Effects 0.000 description 1
- 238000003782 apoptosis assay Methods 0.000 description 1
- 108010072041 arginyl-glycyl-aspartic acid Proteins 0.000 description 1
- 229960001230 asparagine Drugs 0.000 description 1
- 235000009582 asparagine Nutrition 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000031018 biological processes and functions Effects 0.000 description 1
- 239000003114 blood coagulation factor Substances 0.000 description 1
- 229940019700 blood coagulation factors Drugs 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 210000005252 bulbus oculi Anatomy 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 231100000504 carcinogenesis Toxicity 0.000 description 1
- 231100000260 carcinogenicity Toxicity 0.000 description 1
- 230000007670 carcinogenicity Effects 0.000 description 1
- 239000006143 cell culture medium Substances 0.000 description 1
- 239000012578 cell culture reagent Substances 0.000 description 1
- 239000013592 cell lysate Substances 0.000 description 1
- 210000002390 cell membrane structure Anatomy 0.000 description 1
- 210000003855 cell nucleus Anatomy 0.000 description 1
- 230000010307 cell transformation Effects 0.000 description 1
- 210000003169 central nervous system Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 238000010382 chemical cross-linking Methods 0.000 description 1
- 210000004252 chorionic villi Anatomy 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 230000004186 co-expression Effects 0.000 description 1
- 238000012761 co-transfection Methods 0.000 description 1
- 239000003184 complementary RNA Substances 0.000 description 1
- 230000009918 complex formation Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 210000004443 dendritic cell Anatomy 0.000 description 1
- 238000000432 density-gradient centrifugation Methods 0.000 description 1
- 230000030609 dephosphorylation Effects 0.000 description 1
- 238000006209 dephosphorylation reaction Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 238000004520 electroporation Methods 0.000 description 1
- 210000002889 endothelial cell Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 210000000981 epithelium Anatomy 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- DEFVIWRASFVYLL-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl)tetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O DEFVIWRASFVYLL-UHFFFAOYSA-N 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 229960000301 factor viii Drugs 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 102000006815 folate receptor Human genes 0.000 description 1
- 108020005243 folate receptor Proteins 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- PUBCCFNQJQKCNC-XKNFJVFFSA-N gastrin-releasingpeptide Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCSC)C(N)=O)NC(=O)CNC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@H](CC=1N=CNC=1)NC(=O)[C@H](CC(N)=O)NC(=O)CNC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)CNC(=O)[C@H](C)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC(C)C)NC(=O)[C@H]1N(CCC1)C(=O)[C@@H](N)C(C)C)[C@@H](C)O)C(C)C)[C@@H](C)O)C(C)C)C1=CNC=N1 PUBCCFNQJQKCNC-XKNFJVFFSA-N 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000012224 gene deletion Methods 0.000 description 1
- 108091006104 gene-regulatory proteins Proteins 0.000 description 1
- 102000034356 gene-regulatory proteins Human genes 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 230000035931 haemagglutination Effects 0.000 description 1
- 231100000171 higher toxicity Toxicity 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 230000005847 immunogenicity Effects 0.000 description 1
- 238000003364 immunohistochemistry Methods 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 102000008371 intracellularly ATP-gated chloride channel activity proteins Human genes 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 210000004165 myocardium Anatomy 0.000 description 1
- 210000005170 neoplastic cell Anatomy 0.000 description 1
- 230000010309 neoplastic transformation Effects 0.000 description 1
- 230000001537 neural effect Effects 0.000 description 1
- 230000002232 neuromuscular Effects 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 230000006911 nucleation Effects 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 108700025694 p53 Genes Proteins 0.000 description 1
- 238000006552 photochemical reaction Methods 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 230000005522 programmed cell death Effects 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000002731 protein assay Methods 0.000 description 1
- 102000034272 protein filaments Human genes 0.000 description 1
- 108091005974 protein filaments Proteins 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 1
- 230000000284 resting effect Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 108091092562 ribozyme Proteins 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 210000002027 skeletal muscle Anatomy 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 230000002463 transducing effect Effects 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 238000002255 vaccination Methods 0.000 description 1
- 244000057424 walen Species 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/10011—Adenoviridae
- C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
- C12N2710/10341—Use of virus, viral particle or viral elements as a vector
- C12N2710/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/10011—Adenoviridae
- C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
- C12N2710/10351—Methods of production or purification of viral material
- C12N2710/10352—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2810/00—Vectors comprising a targeting moiety
- C12N2810/40—Vectors comprising a peptide as targeting moiety, e.g. a synthetic peptide, from undefined source
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2810/00—Vectors comprising a targeting moiety
- C12N2810/40—Vectors comprising a peptide as targeting moiety, e.g. a synthetic peptide, from undefined source
- C12N2810/405—Vectors comprising RGD peptide
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2810/00—Vectors comprising a targeting moiety
- C12N2810/50—Vectors comprising as targeting moiety peptide derived from defined protein
- C12N2810/60—Vectors comprising as targeting moiety peptide derived from defined protein from viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2810/00—Vectors comprising a targeting moiety
- C12N2810/50—Vectors comprising as targeting moiety peptide derived from defined protein
- C12N2810/60—Vectors comprising as targeting moiety peptide derived from defined protein from viruses
- C12N2810/6009—Vectors comprising as targeting moiety peptide derived from defined protein from viruses dsDNA viruses
- C12N2810/6018—Adenoviridae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2810/00—Vectors comprising a targeting moiety
- C12N2810/50—Vectors comprising as targeting moiety peptide derived from defined protein
- C12N2810/80—Vectors comprising as targeting moiety peptide derived from defined protein from vertebrates
- C12N2810/85—Vectors comprising as targeting moiety peptide derived from defined protein from vertebrates mammalian
- C12N2810/859—Vectors comprising as targeting moiety peptide derived from defined protein from vertebrates mammalian from immunoglobulins
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Manufacturing Of Steel Electrode Plates (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19955558A DE19955558C2 (de) | 1999-11-18 | 1999-11-18 | Permanente Amniozyten-Zelllinie, ihre Herstellung und Verwendung zur Herstellung von Gentransfervektoren |
| PCT/EP2000/010992 WO2001036615A2 (de) | 1999-11-18 | 2000-11-07 | Permanente amniozyten-zelllinie, ihre herstellung und verwendung zur herstellung von gentransfervektoren |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| PL357495A1 PL357495A1 (en) | 2004-07-26 |
| PL205966B1 true PL205966B1 (pl) | 2010-06-30 |
Family
ID=7929523
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PL357495A PL205966B1 (pl) | 1999-11-18 | 2000-11-07 | Ciągła amniocytarna linia komórkowa, sposób jej wytwarzania, zastosowanie amniocytów, zastosowanie cząsteczki kwasu nukleinowego, zastosowanie ciągłej amniocytarnej linii komórkowej i sposób wytwarzania wektorów przenoszących geny i/lub mutantów adenowirusowych |
Country Status (16)
| Country | Link |
|---|---|
| EP (1) | EP1230354B1 (OSRAM) |
| JP (1) | JP4456790B2 (OSRAM) |
| CN (1) | CN100412201C (OSRAM) |
| AT (1) | ATE257512T1 (OSRAM) |
| AU (1) | AU784003B2 (OSRAM) |
| CA (1) | CA2391591C (OSRAM) |
| CZ (1) | CZ300124B6 (OSRAM) |
| DE (2) | DE19955558C2 (OSRAM) |
| DK (1) | DK1230354T3 (OSRAM) |
| ES (1) | ES2211647T3 (OSRAM) |
| HU (1) | HU227440B1 (OSRAM) |
| IL (2) | IL149291A0 (OSRAM) |
| PL (1) | PL205966B1 (OSRAM) |
| PT (1) | PT1230354E (OSRAM) |
| TR (1) | TR200400402T4 (OSRAM) |
| WO (1) | WO2001036615A2 (OSRAM) |
Families Citing this family (71)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1528101A1 (en) * | 2003-11-03 | 2005-05-04 | ProBioGen AG | Immortalized avian cell lines for virus production |
| CN1922308A (zh) | 2004-02-23 | 2007-02-28 | 克鲁塞尔荷兰公司 | 病毒纯化方法 |
| ES2317517T5 (es) | 2005-04-11 | 2016-01-21 | Crucell Holland B.V. | Purificación de virus usando ultrafiltración |
| DE102005054628A1 (de) | 2005-11-16 | 2007-05-24 | Cevec Pharmaceuticals Gmbh | Verfahren zur Herstellung von permanenten humanen Zelllinien |
| PL2061803T5 (pl) | 2006-08-28 | 2023-03-27 | Ares Trading S.A. | Proces oczyszczania białek zawierających fc |
| NZ593235A (en) | 2008-11-03 | 2013-02-22 | Crucell Holland Bv | Method for the production of recombinant adenovirus serotype 35 |
| DE102009003439A1 (de) * | 2009-02-05 | 2010-08-26 | Cevec Pharmaceuticals Gmbh | Neue permanente humane Zelllinie |
| EA028875B1 (ru) | 2009-10-15 | 2018-01-31 | Янссен Вэксинс Энд Превеншн Б.В. | Способ очистки аденовирусных частиц из клеточной суспензии |
| BR112012008516B1 (pt) | 2009-10-15 | 2021-10-19 | Janssen Vaccines & Prevention B.V. | Método para purificar partículas de adenovírus a partir de uma suspensão de célula |
| EA023816B1 (ru) | 2010-02-15 | 2016-07-29 | Круселл Холланд Б.В. | СПОСОБ ПРОДУЦИРОВАНИЯ ВИРУСНЫХ ЧАСТИЦ Ad26 |
| RU2565546C2 (ru) * | 2010-08-16 | 2015-10-20 | Севек Фармасьютикалз Гмбх | Постоянные линии клеток человека для получения вирусов гриппа |
| EP2784155B1 (en) * | 2011-11-24 | 2018-06-13 | Viromed Co. Ltd. | Adenovirus producing novel cell line and the use thereof |
| US8932607B2 (en) | 2012-03-12 | 2015-01-13 | Crucell Holland B.V. | Batches of recombinant adenovirus with altered terminal ends |
| PL2825640T3 (pl) | 2012-03-12 | 2016-10-31 | Partie rekombinowanych adenowirusów o zmienionych końcach | |
| AP2014007993A0 (en) | 2012-03-22 | 2014-10-31 | Crucell Holland Bv | Vaccine against RSV |
| EP2662451A1 (en) * | 2012-05-07 | 2013-11-13 | Stefan Kochanek | Nucleic acid construct and use of the same |
| EP2722337A1 (en) * | 2012-10-19 | 2014-04-23 | CEVEC Pharmaceuticals GmbH | Production of a HCMV based vaccine in human amniocyte cell lines |
| US20150259387A1 (en) * | 2012-10-19 | 2015-09-17 | Cevec Pharmaceuticals Gmbh | Production of a hcmv based vaccine in human amniocyte cell lines |
| US10899800B2 (en) | 2013-04-25 | 2021-01-26 | Janssen Vaccines & Prevention B.V. | Stabilized soluble pre-fusion RSV F polypeptides |
| EP3010931B1 (en) | 2013-06-17 | 2018-06-13 | Janssen Vaccines & Prevention B.V. | Stabilized soluble pre-fusion rsv f polypeptides |
| DE112014003136A5 (de) | 2013-07-01 | 2016-04-21 | Charité - Universitätsmedizin Berlin | Immortalisierte humane Chorionzelllinie sowie Verfahren zur Immortalisierung humaner Chorionzellen |
| CN107075521B (zh) | 2014-11-04 | 2021-06-08 | 扬森疫苗与预防公司 | 治疗性hpv16疫苗 |
| EP3042952A1 (en) | 2015-01-07 | 2016-07-13 | CEVEC Pharmaceuticals GmbH | O-glycan sialylated recombinant glycoproteins and cell lines for producing the same |
| PL3283634T3 (pl) | 2015-04-14 | 2019-10-31 | Janssen Vaccines & Prevention Bv | Rekombinowany adenowirus eksprymujący dwa transgeny z dwukierunkowym promotorem |
| KR102638978B1 (ko) | 2015-07-07 | 2024-02-22 | 얀센 백신스 앤드 프리벤션 비.브이. | Rsv에 대한 백신 |
| KR20180026734A (ko) | 2015-07-07 | 2018-03-13 | 얀센 백신스 앤드 프리벤션 비.브이. | 안정화된 가용성 융합-전 rsv f 폴리펩티드 |
| KR20180042295A (ko) | 2015-08-20 | 2018-04-25 | 얀센 백신스 앤드 프리벤션 비.브이. | 치료용 hpv18 백신 |
| EA201892250A1 (ru) | 2016-04-05 | 2019-03-29 | Янссен Вэксинс Энд Превеншн Б.В. | Вакцина против rsv |
| KR20220041966A (ko) | 2016-04-05 | 2022-04-01 | 얀센 백신스 앤드 프리벤션 비.브이. | 안정화된 용해성 융합-전 rsv f 단백질 |
| WO2017192418A1 (en) | 2016-05-02 | 2017-11-09 | Janssen Vaccine & Prevention B.V. | Therapeutic hpv vaccine combinations |
| WO2017194655A1 (en) | 2016-05-12 | 2017-11-16 | Janssen Vaccines & Prevention B.V. | Potent and balanced bidirectional promoter |
| WO2017207480A1 (en) | 2016-05-30 | 2017-12-07 | Janssen Vaccines & Prevention B.V. | Stabilized pre-fusion rsv f proteins |
| ES2821876T3 (es) | 2016-06-20 | 2021-04-28 | Janssen Vaccines & Prevention Bv | Promotor bidireccional potente y equilibrado |
| JP7229151B2 (ja) | 2016-07-14 | 2023-02-27 | ヤンセン ファッシンズ アンド プリベンション ベーフェー | Ｈｐｖワクチン |
| JP6721797B2 (ja) | 2017-02-09 | 2020-07-15 | ヤンセン ファッシンズ アンド プリベンション ベーフェーＪａｎｓｓｅｎ Ｖａｃｃｉｎｅｓ ＆ Ｐｒｅｖｅｎｔｉｏｎ Ｂ．Ｖ． | 異種遺伝子発現のための強力で短いプロモーター |
| EP3382014A1 (en) | 2017-03-29 | 2018-10-03 | CEVEC Pharmaceuticals GmbH | Recombinant glycoproteins with reduced antennary fucosylation |
| JP2020519663A (ja) | 2017-05-17 | 2020-07-02 | ヤンセン ファッシンズ アンド プリベンション ベーフェーＪａｎｓｓｅｎ Ｖａｃｃｉｎｅｓ ＆ Ｐｒｅｖｅｎｔｉｏｎ Ｂ．Ｖ． | Ｒｓｖ感染に対する防御免疫を誘導するための方法及び組成物 |
| MX2020002876A (es) | 2017-09-15 | 2020-07-22 | Janssen Vaccines & Prevention Bv | Metodo para la induccion segura de inmunidad contra el vsr. |
| EA202091004A1 (ru) | 2017-10-31 | 2020-08-10 | Янссен Вэксинс Энд Превеншн Б.В. | Аденовирус и пути его применения |
| EP3703722B1 (en) | 2017-10-31 | 2024-06-12 | Janssen Vaccines & Prevention B.V. | Adenovirus and uses thereof |
| AU2018360253B2 (en) | 2017-10-31 | 2023-11-09 | Janssen Vaccines & Prevention B.V. | Adenovirus and uses thereof |
| KR102755022B1 (ko) | 2017-10-31 | 2025-01-20 | 얀센 백신스 앤드 프리벤션 비.브이. | 아데노바이러스 벡터 및 이의 용도 |
| JP2022513025A (ja) | 2018-11-13 | 2022-02-07 | ヤンセン ファッシンズ アンド プリベンション ベーフェー | 安定化された融合前ｒｓｖ ｆタンパク質 |
| TWI852977B (zh) | 2019-01-10 | 2024-08-21 | 美商健生生物科技公司 | 前列腺新抗原及其用途 |
| EP3736286A1 (en) | 2019-05-09 | 2020-11-11 | Biotest AG | Single chain factor viii molecule |
| KR20220008816A (ko) | 2019-05-15 | 2022-01-21 | 얀센 백신스 앤드 프리벤션 비.브이. | 아데노바이러스 기반 백신을 사용한 호흡기 세포융합 바이러스 감염의 예방적 치료 |
| CA3140230A1 (en) | 2019-05-15 | 2020-11-19 | Janssen Vaccines & Prevention B.V. | Co-administration of seasonal influenza vaccine and an adenovirus based respiratory syncytial virus vaccine |
| AU2020342349A1 (en) | 2019-09-02 | 2022-03-03 | Biotest Ag | Factor VIII protein with increased half-life |
| EP3785726A1 (en) | 2019-09-02 | 2021-03-03 | Biotest AG | Factor viii protein with increased half-life |
| IL291852A (en) | 2019-10-03 | 2022-06-01 | Janssen Vaccines & Prevention Bv | Adenovirus vectors and their use |
| CA3161800A1 (en) | 2019-11-18 | 2021-05-27 | Janssen Biotech, Inc. | Vaccines based on mutant calr and jak2 and their uses |
| TW202144388A (zh) | 2020-02-14 | 2021-12-01 | 美商健生生物科技公司 | 在卵巢癌中表現之新抗原及其用途 |
| TW202144389A (zh) | 2020-02-14 | 2021-12-01 | 美商健生生物科技公司 | 在多發性骨髓瘤中表現之新抗原及其用途 |
| US20230190881A1 (en) | 2020-02-17 | 2023-06-22 | Biotest Ag | Subcutaneous administration of factor viii |
| WO2022009052A2 (en) | 2020-07-06 | 2022-01-13 | Janssen Biotech, Inc. | Prostate neoantigens and their uses |
| EP4175721A1 (en) | 2020-07-06 | 2023-05-10 | Janssen Biotech, Inc. | Prostate neoantigens and their uses |
| US20230035403A1 (en) | 2020-07-06 | 2023-02-02 | Janssen Biotech, Inc. | Method For Determining Responsiveness To Prostate Cancer Treatment |
| KR20230085170A (ko) | 2020-10-15 | 2023-06-13 | 에프. 호프만-라 로슈 아게 | 동시 유전자 활성화를 위한 핵산 구조체 |
| EP4229205B1 (en) | 2020-10-15 | 2025-05-14 | F. Hoffmann-La Roche AG | Nucleic acid constructs for va rna transcription |
| TW202323529A (zh) | 2021-10-18 | 2023-06-16 | 美商再生元醫藥公司 | 包括腺病毒相關病毒聚核苷酸的真核細胞 |
| EP4508241A1 (en) | 2022-04-13 | 2025-02-19 | F. Hoffmann-La Roche AG | Method for determining aav genomes |
| EP4529614A1 (en) | 2022-05-23 | 2025-04-02 | F. Hoffmann-La Roche AG | Raman-based method for the differentiation of aav particle serotype and aav particle loading status |
| JP2025519228A (ja) | 2022-06-03 | 2025-06-24 | エフ． ホフマン－ラ ロシュ アーゲー | 組換えａａｖ粒子を生成するための方法 |
| WO2024013239A1 (en) | 2022-07-14 | 2024-01-18 | F. Hoffmann-La Roche Ag | Method for producing recombinant aav particles |
| JP2025530294A (ja) | 2022-09-12 | 2025-09-11 | エフ． ホフマン－ラ ロシュ アーゲー | 完全なａａｖ粒子と空のａａｖ粒子を分離するための方法 |
| KR20250141727A (ko) | 2023-02-07 | 2025-09-29 | 에프. 호프만-라 로슈 아게 | 항-aav 입자 항체 검출 방법 |
| AU2024239386A1 (en) | 2023-03-21 | 2025-08-14 | F. Hoffmann-La Roche Ag | Method for the production of recombinant aav particle preparations |
| CN117487761A (zh) * | 2023-09-25 | 2024-02-02 | 南京农业大学 | 表达Cre基因猪骨髓巨噬细胞系的构建方法及其应用 |
| WO2025158316A1 (en) | 2024-01-26 | 2025-07-31 | Janssen Biotech, Inc. | Ovarian neoantigens and use thereof |
| WO2025168663A1 (en) | 2024-02-09 | 2025-08-14 | F. Hoffmann-La Roche Ag | Method for producing recombinant adeno-associated viral particles |
| WO2025252480A1 (en) | 2024-06-07 | 2025-12-11 | F. Hoffmann-La Roche Ag | Method for purifying plasmid dna |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE69638058D1 (de) * | 1995-06-15 | 2009-11-26 | Crucell Holland Bv | Verpackungssysteme für humane rekombinante Adenoviren zur Gentherapie |
-
1999
- 1999-11-18 DE DE19955558A patent/DE19955558C2/de not_active Expired - Fee Related
-
2000
- 2000-11-07 WO PCT/EP2000/010992 patent/WO2001036615A2/de not_active Ceased
- 2000-11-07 HU HU0203387A patent/HU227440B1/hu not_active IP Right Cessation
- 2000-11-07 TR TR2004/00402T patent/TR200400402T4/xx unknown
- 2000-11-07 EP EP00979539A patent/EP1230354B1/de not_active Expired - Lifetime
- 2000-11-07 IL IL14929100A patent/IL149291A0/xx active IP Right Grant
- 2000-11-07 DE DE50004995T patent/DE50004995D1/de not_active Expired - Lifetime
- 2000-11-07 CZ CZ20021709A patent/CZ300124B6/cs not_active IP Right Cessation
- 2000-11-07 AU AU16990/01A patent/AU784003B2/en not_active Expired
- 2000-11-07 CN CNB008159017A patent/CN100412201C/zh not_active Expired - Lifetime
- 2000-11-07 DK DK00979539T patent/DK1230354T3/da active
- 2000-11-07 AT AT00979539T patent/ATE257512T1/de active
- 2000-11-07 CA CA002391591A patent/CA2391591C/en not_active Expired - Lifetime
- 2000-11-07 PT PT00979539T patent/PT1230354E/pt unknown
- 2000-11-07 ES ES00979539T patent/ES2211647T3/es not_active Expired - Lifetime
- 2000-11-07 JP JP2001538494A patent/JP4456790B2/ja not_active Expired - Lifetime
- 2000-11-07 PL PL357495A patent/PL205966B1/pl unknown
-
2002
- 2002-04-23 IL IL149291A patent/IL149291A/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| ATE257512T1 (de) | 2004-01-15 |
| CZ20021709A3 (cs) | 2002-08-14 |
| CA2391591A1 (en) | 2001-05-25 |
| DE19955558C2 (de) | 2003-03-20 |
| IL149291A0 (en) | 2002-11-10 |
| CA2391591C (en) | 2008-12-30 |
| DE50004995D1 (de) | 2004-02-12 |
| WO2001036615A2 (de) | 2001-05-25 |
| HUP0203387A2 (hu) | 2002-12-28 |
| IL149291A (en) | 2008-11-26 |
| WO2001036615A3 (de) | 2002-01-10 |
| HUP0203387A3 (en) | 2005-07-28 |
| CN100412201C (zh) | 2008-08-20 |
| DK1230354T3 (da) | 2004-03-22 |
| CZ300124B6 (cs) | 2009-02-18 |
| DE19955558A1 (de) | 2001-06-07 |
| EP1230354A2 (de) | 2002-08-14 |
| EP1230354B1 (de) | 2004-01-07 |
| JP2003514526A (ja) | 2003-04-22 |
| TR200400402T4 (tr) | 2004-03-22 |
| CN1433476A (zh) | 2003-07-30 |
| JP4456790B2 (ja) | 2010-04-28 |
| AU1699001A (en) | 2001-05-30 |
| PT1230354E (pt) | 2004-04-30 |
| ES2211647T3 (es) | 2004-07-16 |
| AU784003B2 (en) | 2006-01-12 |
| PL357495A1 (en) | 2004-07-26 |
| HU227440B1 (en) | 2011-06-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU784003B2 (en) | Permanent amniocyte cell line, the production thereof and its use for producing gene transfer vectors | |
| US6558948B1 (en) | Permanent amniocytic cell line, its production and use for the production of gene transfer vectors | |
| US20090098599A1 (en) | Adenovirus vectors, packaging cell lines, compositions, and methods for preparation and use | |
| Wu et al. | A 50-kDa membrane protein mediates sialic acid-independent binding and infection of conjunctival cells by adenovirus type 37 | |
| KR20010034487A (ko) | 섬유 혹의 ｈｉ 루프내에 이종성 펩타이드 에피토프를함유하는 아데노바이러스 벡터 | |
| EP1280929A2 (en) | Vectors for ocular transduction and use thereof for genetic therapy | |
| US7456008B2 (en) | Modified virus comprising one or more non-native polypeptides | |
| US20110189234A1 (en) | Adenovirus particles having a chimeric adenovirus spike protein, use thereof and methods for producing such particles | |
| AU2001270879A1 (en) | Modified virus having altered tropism | |
| EP1141357A1 (en) | Adenovirus vectors, packaging cell lines, compositions, and methods for preparation and use | |
| Lucas et al. | Hexon modification to improve the activity of oncolytic adenovirus vectors against neoplastic and stromal cells in pancreatic cancer | |
| KR20190118163A (ko) | 가족성 고콜레스테롤혈증을 치료하기 위한 유전자 요법 | |
| Murakami et al. | Chimeric adenoviral vectors incorporating a fiber of human adenovirus 3 efficiently mediate gene transfer into prostate cancer cells | |
| KR102208879B1 (ko) | 핵산 구조 및 이의 용도 | |
| CN113462657A (zh) | 重组新城疫病毒及制备方法、重组质粒、及其应用 | |
| DE29924144U1 (de) | Permanente Amniozyten-Zelllinie | |
| de Vrij et al. | Enhanced transduction of CAR-negative cells by protein IX-gene deleted adenovirus 5 vectors | |
| Mei et al. | Highly heterogeneous fiber genes in the two closely related adenovirus genome types Ad35p and Ad34a | |
| CN113462658A (zh) | 重组新城疫病毒及制备方法、重组质粒、及其应用 | |
| HK40061867A (en) | Recombinant newcastle disease virus, preparation method, recombinant plasmid and application thereof | |
| CN109082443A (zh) | 一种制备可实时检测间充质干细胞向成熟肝样细胞分化的细胞模型的方法 | |
| HK40061868A (en) | Recombinant newcastle disease virus, preparation method, recombinant plasmid and application of recombinant newcastle disease virus | |
| Class et al. | Patent application title: NUCLEIC ACID CONSTRUCT AND USE OF THE SAME Inventors: Stefan Kochanek (Ulm, DE) Stefan Kochanek Tanja Lucas (Regensburg, DE) Claudia Kueppers (Weil Am Rhein, DE) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RECP | Rectifications of patent specification |