PE20120571A1 - Produccion de titulos elevados de polivirus para la produccion de vacunas - Google Patents

Produccion de titulos elevados de polivirus para la produccion de vacunas

Info

Publication number
PE20120571A1
PE20120571A1 PE2012000051A PE2012000051A PE20120571A1 PE 20120571 A1 PE20120571 A1 PE 20120571A1 PE 2012000051 A PE2012000051 A PE 2012000051A PE 2012000051 A PE2012000051 A PE 2012000051A PE 20120571 A1 PE20120571 A1 PE 20120571A1
Authority
PE
Peru
Prior art keywords
poliovirus
production
cells
polivirus
titles
Prior art date
Application number
PE2012000051A
Other languages
English (en)
Inventor
John Alfred Lewis
Original Assignee
Crucell Holland Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Crucell Holland Bv filed Critical Crucell Holland Bv
Publication of PE20120571A1 publication Critical patent/PE20120571A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C12N7/02—Recovery or purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/125—Picornaviridae, e.g. calicivirus
    • A61K39/13—Poliovirus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/32011—Picornaviridae
    • C12N2770/32611—Poliovirus
    • C12N2770/32634—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/32011—Picornaviridae
    • C12N2770/32611—Poliovirus
    • C12N2770/32651—Methods of production or purification of viral material
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Epidemiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Molecular Biology (AREA)
  • Neurosurgery (AREA)
  • Neurology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

UN PROCESO PARA PRODUCIR UN POLIOVIRUS QUE COMPRENDE PROVEER UN CULTIVO DE CELULAS PER.C6 EN UNA SUSPENSION LIBRE DE SUERO, INFECTAR DICHAS CELULAS CON POLIOVIRUS EN UNA DENSIDAD CELULAR DE ENTRE 2X106 CELULAS/ML Y 150X106 CELULAS/ML Y COSECHAR LOS POLIOVIRUS ENTRE 12 Y 48 HORAS DESPUES DE LA INFECCION. EL POLIOVIRUS ES LA CEPA MAHONEY DEL POLIOVIRUS DEL TIPO 1, LA CEPA MEF DEL POLIOVIRUS DEL TIPO 2 O LA CEPA SAUKETT DEL POLIOVIRUS DEL TIPO 3. TAMBIEN ESTA REFERIDA A UNA VACUNA CONTRA LA POLIO QUE COMPRENDE A DICHO POLIOVIRUS, PURIFICADO Y OPCIONALMENTE, INACTIVADO
PE2012000051A 2009-07-16 2010-07-08 Produccion de titulos elevados de polivirus para la produccion de vacunas PE20120571A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US27103809P 2009-07-16 2009-07-16
EP09165620 2009-07-16

Publications (1)

Publication Number Publication Date
PE20120571A1 true PE20120571A1 (es) 2012-06-06

Family

ID=41412431

Family Applications (1)

Application Number Title Priority Date Filing Date
PE2012000051A PE20120571A1 (es) 2009-07-16 2010-07-08 Produccion de titulos elevados de polivirus para la produccion de vacunas

Country Status (33)

Country Link
US (4) US8546123B2 (es)
EP (1) EP2454364B1 (es)
JP (1) JP5845178B2 (es)
KR (1) KR101548790B1 (es)
CN (1) CN102482647B (es)
AP (1) AP3140A (es)
AR (1) AR077314A1 (es)
AU (1) AU2010272685B2 (es)
BR (1) BR112012000942B8 (es)
CA (1) CA2763091C (es)
CO (1) CO6491042A2 (es)
CU (1) CU20120006A7 (es)
DK (1) DK2454364T3 (es)
EA (1) EA020563B1 (es)
ES (1) ES2484093T3 (es)
HK (1) HK1164922A1 (es)
HR (1) HRP20140597T1 (es)
IL (1) IL217465A (es)
MA (1) MA33429B1 (es)
MX (1) MX2011012648A (es)
MY (1) MY183385A (es)
NZ (1) NZ596880A (es)
PE (1) PE20120571A1 (es)
PL (1) PL2454364T3 (es)
PT (1) PT2454364E (es)
RS (1) RS53377B (es)
SG (1) SG177655A1 (es)
SI (1) SI2454364T1 (es)
SM (1) SMT201400079B (es)
TN (1) TN2011000628A1 (es)
TW (1) TWI477606B (es)
WO (1) WO2011006823A1 (es)
ZA (1) ZA201108271B (es)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2647985C (en) 2006-03-31 2014-12-30 Warf-Wisconsin Alumni Research Foundation High titer recombinant influenza viruses for vaccines
US9474798B2 (en) 2007-06-18 2016-10-25 Wisconsin Alumni Research Foundation Influenza M2 protein mutant viruses as live influenza attenuated vaccines
BRPI0921651A2 (pt) 2008-11-03 2015-08-18 Crucell Holland Bv Método para produzir adenovírus recombinante, e partículas virais, e, biorreator
SG177655A1 (en) 2009-07-16 2012-02-28 Crucell Holland Bv Production of polio virus at high titers for vaccine production
US9109013B2 (en) 2009-10-26 2015-08-18 Wisconsin Alumni Research Foundation High titer recombinant influenza viruses with enhanced replication in vero cells
US10130697B2 (en) 2010-03-23 2018-11-20 Wisconsin Alumni Research Foundation (Warf) Vaccines comprising mutant attenuated influenza viruses
AU2014215025B2 (en) 2013-02-05 2018-08-30 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Cell lines for virus production and methods of use
WO2014135651A1 (en) 2013-03-08 2014-09-12 Crucell Holland B.V. Acellular pertussis vaccine
WO2014142433A1 (ko) 2013-03-13 2014-09-18 고려대학교산학협력단 바이러스 생산능이 증가된 세포주 및 그 제조방법
KR20140112255A (ko) 2013-03-13 2014-09-23 고려대학교 산학협력단 바이러스 생산능이 증가된 세포주 및 그 제조방법
GB201305361D0 (en) 2013-03-25 2013-05-08 Univ Edinburgh Enhanced expression
RS57864B1 (sr) 2013-06-17 2018-12-31 De Staat Der Nederlanden Vert Door De Minister Van Vws Ministerie Van Volksgezondheid Welzijn En Spo Postupci za sprečavanje agregacije virusnih komponenti
JP2016524915A (ja) 2013-07-15 2016-08-22 ウィスコンシン アルムニ リサーチ ファンデイション Ｍｄｃｋ、ベロ細胞又は卵内で増強された複製を有する高力価の組換えインフルエンザウイルス
US20160053281A1 (en) * 2013-08-15 2016-02-25 The University Court Of The University Of Edinburgh Enhanced Expression Of RNA Vectors
CN106232800B (zh) 2014-03-25 2020-07-03 泰尔茂比司特公司 介质的被动替代
AU2015276235B2 (en) 2014-06-17 2021-02-18 Janssen Vaccines & Prevention B.V. Cold-Adapted-Viral-Attenuation (CAVA) and novel attenuated Poliovirus strains
US10526393B2 (en) 2014-06-18 2020-01-07 Immunomax Co., Ltd. Method for promoting virus infection and increasing virus production, by using cell line having lost BST2 gene functions
US10053671B2 (en) 2014-06-20 2018-08-21 Wisconsin Alumni Research Foundation (Warf) Mutations that confer genetic stability to additional genes in influenza viruses
EP3172317B1 (en) 2014-07-24 2019-05-01 Janssen Vaccines & Prevention B.V. Process for the purification of poliovirus from cell cultures
US10633422B2 (en) 2015-06-01 2020-04-28 Wisconsin Alumni Research Foundation (Warf) Influenza virus replication by inhibiting microRNA lec7C binding to influenza viral cRNA and mRNA
WO2017004592A1 (en) 2015-07-02 2017-01-05 Terumo Bct, Inc. Cell growth with mechanical stimuli
US9890363B2 (en) 2015-07-06 2018-02-13 Wisconsin Alumni Research Foundation (Warf) Influenza virus replication for vaccine development
JP2019510481A (ja) 2016-02-19 2019-04-18 ウィスコンシン アルムニ リサーチ ファンデイション ワクチン開発のための改善されたｂ型インフルエンザウイルス複製
WO2017205667A1 (en) 2016-05-25 2017-11-30 Terumo Bct, Inc. Cell expansion
US11104874B2 (en) 2016-06-07 2021-08-31 Terumo Bct, Inc. Coating a bioreactor
US11685883B2 (en) 2016-06-07 2023-06-27 Terumo Bct, Inc. Methods and systems for coating a cell growth surface
US10372100B2 (en) * 2016-08-29 2019-08-06 Ge Healthcare Bio-Sciences Corp. Manufacturing system for biopharmaceutical products
CN110612344B (zh) 2017-03-31 2023-09-12 泰尔茂比司特公司 细胞扩增
CN107723279B (zh) * 2017-10-18 2020-11-10 成都远睿生物技术有限公司 一种缺陷型腺病毒AdC68-GP的培养方法
WO2019155492A1 (en) * 2018-02-07 2019-08-15 Bharat Biotech International Limited A process for enterovirus purification and inactivation and vaccine compositions obtained thereof
US11241492B2 (en) 2019-01-23 2022-02-08 Wisconsin Alumni Research Foundation (Warf) Mutations that confer genetic stability to genes in influenza viruses
US11851648B2 (en) 2019-02-08 2023-12-26 Wisconsin Alumni Research Foundation (Warf) Humanized cell line
WO2020223699A1 (en) 2019-05-01 2020-11-05 Wisconsin Alumni Research Foundation (Warf) Improved influenza virus replication for vaccine development
WO2020229581A1 (en) 2019-05-16 2020-11-19 Janssen Vaccines & Prevention B.V. Methods for inducing a safe immune response against polio virus
WO2021041624A2 (en) 2019-08-27 2021-03-04 Yoshihiro Kawaoka Recombinant influenza viruses with stabilized ha for replication in eggs

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4525349A (en) * 1981-12-29 1985-06-25 Societe Anonyme Dite: Institut Merueux Process for the large-scale production of a vaccine against poliomyelitis and the resulting vaccine
EP0833934B2 (en) 1995-06-15 2012-08-15 Crucell Holland B.V. Packaging systems for human recombinant adenovirus to be used in gene therapy
US7521220B2 (en) * 1999-11-26 2009-04-21 Crucell Holland B.V. Production of vaccines
EP1103610A1 (en) 1999-11-26 2001-05-30 Introgene B.V. Production of vaccines from immortalised mammalian cell lines
US6544424B1 (en) 1999-12-03 2003-04-08 Refined Technology Company Fluid filtration system
EP1256803A1 (en) 2001-05-07 2002-11-13 Crucell Holland B.V. Methods for the identification of antiviral compounds
AU2003288273A1 (en) * 2002-10-23 2004-05-13 Crucell Holland B.V. New settings for recombinant adenoviral-based vaccines
NZ539813A (en) * 2002-12-17 2008-04-30 Crucell Holland Bv A replication defective recombinant adenovirus comprising a antigenic determinant of Plasmodium falciparum wherein the antigenic determinant is SEQ ID 6
CN101328473B (zh) * 2003-05-09 2012-05-02 克鲁塞尔荷兰公司 E1-永生化的细胞培养物及培养所述细胞以提高从中获取的产物产量的方法
CN1930281A (zh) 2004-03-05 2007-03-14 帝斯曼知识产权资产管理有限公司 用于通过连续灌注和交互切向流来培养细胞的方法
EA010057B1 (ru) 2004-08-27 2008-06-30 Панацея Биотек Лтд. Инактивированная полиомиелитная вакцина, полученная из штамма сейбина вируса полиомиелита
ATE523205T1 (de) * 2004-10-14 2011-09-15 Crucell Holland Bv Prime-boost-impfstoffe gegen malaria
CN100500827C (zh) * 2004-11-26 2009-06-17 中国医学科学院医学生物学研究所 用人胚肺二倍体细胞培养繁殖脊髓灰质炎病毒的方法
US20100144620A1 (en) * 2006-11-16 2010-06-10 Crucell Holland B.V. Complementation of factor xi deficeincy by factor v mutants
SG177655A1 (en) * 2009-07-16 2012-02-28 Crucell Holland Bv Production of polio virus at high titers for vaccine production
KR20120070015A (ko) 2010-12-21 2012-06-29 (주)창조바이오텍 알파-피넨을 유효성분으로 포함하는 스쿠티카충 구제용 조성물

Also Published As

Publication number Publication date
AP3140A (en) 2015-02-28
CO6491042A2 (es) 2012-07-31
CU20120006A7 (es) 2012-10-15
EA020563B1 (ru) 2014-12-30
MX2011012648A (es) 2012-01-12
TN2011000628A1 (en) 2013-05-24
US20130052224A1 (en) 2013-02-28
AP2012006075A0 (en) 2012-02-29
US20140242670A1 (en) 2014-08-28
US20130273106A1 (en) 2013-10-17
EP2454364A1 (en) 2012-05-23
MA33429B1 (fr) 2012-07-03
US20110027317A1 (en) 2011-02-03
EA201270173A1 (ru) 2012-06-29
NZ596880A (en) 2013-10-25
BR112012000942B8 (pt) 2021-05-25
DK2454364T3 (da) 2014-07-21
IL217465A0 (en) 2012-02-29
SMT201400079B (it) 2014-09-08
CN102482647B (zh) 2015-12-16
CA2763091A1 (en) 2011-01-20
AR077314A1 (es) 2011-08-17
AU2010272685A1 (en) 2011-12-08
HK1164922A1 (en) 2012-09-28
CA2763091C (en) 2019-07-23
MY183385A (en) 2021-02-18
CN102482647A (zh) 2012-05-30
ES2484093T3 (es) 2014-08-11
KR20120033334A (ko) 2012-04-06
WO2011006823A1 (en) 2011-01-20
EP2454364B1 (en) 2014-04-23
PL2454364T3 (pl) 2014-09-30
SI2454364T1 (sl) 2014-08-29
JP5845178B2 (ja) 2016-01-20
JP2012532616A (ja) 2012-12-20
HRP20140597T1 (hr) 2014-09-12
TWI477606B (zh) 2015-03-21
IL217465A (en) 2016-05-31
BR112012000942B1 (pt) 2020-10-27
US8546123B2 (en) 2013-10-01
PT2454364E (pt) 2014-07-04
ZA201108271B (en) 2012-07-25
TW201118173A (en) 2011-06-01
AU2010272685B2 (en) 2015-02-26
US9022240B2 (en) 2015-05-05
RS53377B (en) 2014-10-31
BR112012000942A2 (pt) 2016-03-15
SG177655A1 (en) 2012-02-28
KR101548790B1 (ko) 2015-08-31

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