ES2351296B8 - Cepas de s. cerevisiae capaces de crecer en medios con melibiosa, estaquiosa y rafinosa. - Google Patents

Cepas de s. cerevisiae capaces de crecer en medios con melibiosa, estaquiosa y rafinosa. Download PDF

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Publication number
ES2351296B8
ES2351296B8 ES200900961A ES200900961A ES2351296B8 ES 2351296 B8 ES2351296 B8 ES 2351296B8 ES 200900961 A ES200900961 A ES 200900961A ES 200900961 A ES200900961 A ES 200900961A ES 2351296 B8 ES2351296 B8 ES 2351296B8
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Spain
Prior art keywords
cerevisiae
media
staquious
rafinosa
capacies
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Active
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ES200900961A
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English (en)
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ES2351296B1 (es
ES2351296A1 (es
Inventor
Manuel Becerra Fernandez
Maria Esperanza Cerdan Villanueva
Rafael Fernandez Leiro
Maria Isabel Gonzalez Siso
Angel Pereira Rodriguez
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Universidade da Coruna
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Universidade da Coruna
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Publication date
Priority to ES200900961A priority Critical patent/ES2351296B8/es
Application filed by Universidade da Coruna filed Critical Universidade da Coruna
Priority to CN2010800233001A priority patent/CN102449145A/zh
Priority to US13/263,292 priority patent/US20130273602A1/en
Priority to EP10761222A priority patent/EP2418275A4/en
Priority to PCT/ES2010/070219 priority patent/WO2010116020A1/es
Priority to BRPI1012587A priority patent/BRPI1012587A2/pt
Publication of ES2351296A1 publication Critical patent/ES2351296A1/es
Publication of ES2351296B1 publication Critical patent/ES2351296B1/es
Application granted granted Critical
Publication of ES2351296B8 publication Critical patent/ES2351296B8/es
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2465—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on alpha-galactose-glycoside bonds, e.g. alpha-galactosidase (3.2.1.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01022—Alpha-galactosidase (3.2.1.22)

Abstract

La invención se relaciona con cepas de S. cerevisiae capaces de secretar alfa-galactosidasa al medio de cultivo, así como a métodos para la obtención de alfa-galactosidasa utilizando dichas cepas y métodos para la producción de biomasa y bioetanol mediante el cultivo de dichas cepas en medios ricos en galactosa. En otro aspecto, la invención proporciona métodos para la expresión de proteínas recombinantes usando una composición rica en galactosa como medio de cultivo para los microorganismos productores de dicha proteína.
ES200900961A 2009-04-08 2009-04-08 Cepas de s. cerevisiae capaces de crecer en medios con melibiosa, estaquiosa y rafinosa. Active ES2351296B8 (es)

Priority Applications (6)

Application Number Priority Date Filing Date Title
ES200900961A ES2351296B8 (es) 2009-04-08 2009-04-08 Cepas de s. cerevisiae capaces de crecer en medios con melibiosa, estaquiosa y rafinosa.
US13/263,292 US20130273602A1 (en) 2009-04-08 2010-04-08 Strains of s.cerevisiae capable of growing in media with melibiose, stachyose and raffinose
EP10761222A EP2418275A4 (en) 2009-04-08 2010-04-08 STRAINS OF S. CEREVISIAE WHICH CAN DEVELOP IN MEDIUM-RICH MEDIUM, STACHYOSE AND RAFFINOSE MEDIA &xA;
PCT/ES2010/070219 WO2010116020A1 (es) 2009-04-08 2010-04-08 Cepas de s.cerevisiae capaces de crecer en medios con melibiosa, estaquiosa y rafinosa
CN2010800233001A CN102449145A (zh) 2009-04-08 2010-04-08 能够在具有蜜二糖、水苏四糖和棉子糖的培养基中生长的酿酒酵母菌株
BRPI1012587A BRPI1012587A2 (pt) 2009-04-08 2010-04-08 cepas de s. cerevisiae capazes de crescer em meio com melibiose, estaquiose e rafinose

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES200900961A ES2351296B8 (es) 2009-04-08 2009-04-08 Cepas de s. cerevisiae capaces de crecer en medios con melibiosa, estaquiosa y rafinosa.

Publications (3)

Publication Number Publication Date
ES2351296A1 ES2351296A1 (es) 2011-02-02
ES2351296B1 ES2351296B1 (es) 2012-01-02
ES2351296B8 true ES2351296B8 (es) 2012-07-03

Family

ID=42935678

Family Applications (1)

Application Number Title Priority Date Filing Date
ES200900961A Active ES2351296B8 (es) 2009-04-08 2009-04-08 Cepas de s. cerevisiae capaces de crecer en medios con melibiosa, estaquiosa y rafinosa.

Country Status (6)

Country Link
US (1) US20130273602A1 (es)
EP (1) EP2418275A4 (es)
CN (1) CN102449145A (es)
BR (1) BRPI1012587A2 (es)
ES (1) ES2351296B8 (es)
WO (1) WO2010116020A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103695326A (zh) * 2014-01-03 2014-04-02 湖南大学 一种处理分散式高浓度有机废水的酵母及其制备方法与应用
CN103981171A (zh) * 2014-05-12 2014-08-13 江南大学 一种基于酿酒酵母孢子的微胶囊固定化酶的制备方法
CN103966196A (zh) * 2014-05-12 2014-08-06 江南大学 一种利用酿酒酵母二酪氨酸层疏松型孢子固定化酶的方法
CA3135164A1 (en) * 2019-04-02 2020-10-08 Spiber Inc. Method for producing recombinant protein
CN110331115A (zh) * 2019-08-08 2019-10-15 河南省科学院生物研究所有限责任公司 一种用于α-半乳糖苷酶产生菌快速筛选的培养基及其应用

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3846239A (en) 1972-07-24 1974-11-05 Monsanto Co Process for the preparation of heat-resistant alpha-galactosidase enzyme
IT1140312B (it) 1981-12-03 1986-09-24 Anic Spa Procedimento per la produzione di alfa-galattosidasi e impieghi dell'enzima cosi' ottenuto
US7198919B1 (en) * 1983-04-25 2007-04-03 Genentech, Inc. Use of alpha factor sequences in yeast expression systems
CA1252256A (en) * 1984-12-24 1989-04-11 Joel B. Barlow Drain assembly with synthetic resin lock nut and coupling elements
CA1339101C (en) * 1986-06-03 1997-07-29 Nicolaas Overbeeke Production of guar alpha-galactosidase and immunologically related alpha-galactosidases by host organisms transformed with recombinant dna methods
US5055401A (en) * 1987-04-10 1991-10-08 Alko Ltd. Construction of new α-galactosidase producing yeast strains and the industrial application of these strains
US5063154A (en) 1987-06-24 1991-11-05 Whitehead Institute For Biomedical Research Pheromone - inducible yeast promoter
DK38893D0 (da) 1993-03-31 1993-03-31 Novo Nordisk As Dna
ES2172380B1 (es) 2000-03-03 2003-12-01 Consejo Superior Investigacion Un procedimiento para producir alfa-galactosidasa recombinante de thermus sp. t2 en celulas hospedantes.

Also Published As

Publication number Publication date
ES2351296B1 (es) 2012-01-02
CN102449145A (zh) 2012-05-09
WO2010116020A1 (es) 2010-10-14
US20130273602A1 (en) 2013-10-17
BRPI1012587A2 (pt) 2015-09-22
EP2418275A1 (en) 2012-02-15
ES2351296A1 (es) 2011-02-02
EP2418275A4 (en) 2012-09-12

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