NZ593962A - Barley with reduced lipoxygenase activity and beverage prepared therefrom - Google Patents
Barley with reduced lipoxygenase activity and beverage prepared therefromInfo
- Publication number
- NZ593962A NZ593962A NZ593962A NZ59396209A NZ593962A NZ 593962 A NZ593962 A NZ 593962A NZ 593962 A NZ593962 A NZ 593962A NZ 59396209 A NZ59396209 A NZ 59396209A NZ 593962 A NZ593962 A NZ 593962A
- Authority
- NZ
- New Zealand
- Prior art keywords
- lox
- barley
- plant
- beverage
- malt
- Prior art date
Links
- 235000007340 Hordeum vulgare Nutrition 0.000 title claims abstract description 360
- 235000013361 beverage Nutrition 0.000 title claims abstract description 168
- 108090000128 Lipoxygenases Proteins 0.000 title claims abstract description 76
- 102000003820 Lipoxygenases Human genes 0.000 title claims abstract description 75
- 240000005979 Hordeum vulgare Species 0.000 title claims description 488
- 230000000694 effects Effects 0.000 title claims description 163
- 230000002829 reductive effect Effects 0.000 title description 17
- 101710199789 Oxidized low-density lipoprotein receptor 1 Proteins 0.000 claims abstract description 231
- 102100025386 Oxidized low-density lipoprotein receptor 1 Human genes 0.000 claims abstract description 221
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 165
- 102000004190 Enzymes Human genes 0.000 claims abstract description 107
- 108090000790 Enzymes Proteins 0.000 claims abstract description 107
- 241000209219 Hordeum Species 0.000 claims abstract description 81
- 150000001413 amino acids Chemical class 0.000 claims abstract description 36
- 241000196324 Embryophyta Species 0.000 claims description 202
- 235000013405 beer Nutrition 0.000 claims description 194
- 239000000203 mixture Substances 0.000 claims description 158
- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical group CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 claims description 155
- 238000000034 method Methods 0.000 claims description 140
- 230000035772 mutation Effects 0.000 claims description 131
- 210000002257 embryonic structure Anatomy 0.000 claims description 71
- 239000000284 extract Substances 0.000 claims description 59
- 235000019640 taste Nutrition 0.000 claims description 47
- 238000005360 mashing Methods 0.000 claims description 39
- 238000003860 storage Methods 0.000 claims description 33
- 230000015572 biosynthetic process Effects 0.000 claims description 25
- 108020004705 Codon Proteins 0.000 claims description 24
- LSNNMFCWUKXFEE-UHFFFAOYSA-N Sulfurous acid Chemical compound OS(O)=O LSNNMFCWUKXFEE-UHFFFAOYSA-N 0.000 claims description 21
- 108020004485 Nonsense Codon Proteins 0.000 claims description 20
- 238000009395 breeding Methods 0.000 claims description 19
- 230000001488 breeding effect Effects 0.000 claims description 19
- 238000000855 fermentation Methods 0.000 claims description 13
- 230000004151 fermentation Effects 0.000 claims description 13
- 239000002773 nucleotide Substances 0.000 claims description 12
- 125000003729 nucleotide group Chemical group 0.000 claims description 12
- 238000012545 processing Methods 0.000 claims description 10
- 235000021577 malt beverage Nutrition 0.000 claims description 9
- 235000020357 syrup Nutrition 0.000 claims description 9
- 239000006188 syrup Substances 0.000 claims description 9
- 108091081024 Start codon Proteins 0.000 claims description 5
- 230000001476 alcoholic effect Effects 0.000 claims description 4
- 235000020429 malt syrup Nutrition 0.000 claims description 3
- BSAIUMLZVGUGKX-BQYQJAHWSA-N (E)-non-2-enal Chemical compound CCCCCC\C=C\C=O BSAIUMLZVGUGKX-BQYQJAHWSA-N 0.000 description 443
- 229940088598 enzyme Drugs 0.000 description 96
- 239000000047 product Substances 0.000 description 83
- 235000018102 proteins Nutrition 0.000 description 56
- 102000004169 proteins and genes Human genes 0.000 description 56
- 235000013339 cereals Nutrition 0.000 description 53
- 238000004458 analytical method Methods 0.000 description 50
- 230000006870 function Effects 0.000 description 45
- 108020004414 DNA Proteins 0.000 description 43
- 239000002243 precursor Substances 0.000 description 38
- 210000004027 cell Anatomy 0.000 description 36
- 238000004890 malting Methods 0.000 description 36
- 235000020778 linoleic acid Nutrition 0.000 description 35
- OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 description 35
- 239000000796 flavoring agent Substances 0.000 description 34
- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 description 33
- 239000013615 primer Substances 0.000 description 32
- 239000002994 raw material Substances 0.000 description 30
- 238000004519 manufacturing process Methods 0.000 description 29
- 238000006243 chemical reaction Methods 0.000 description 28
- JGUNZIWGNMQSBM-SIGMCMEVSA-N E,E-9-HpODE Chemical compound CCCCC\C=C\C=C\C(OO)CCCCCCCC(O)=O JGUNZIWGNMQSBM-SIGMCMEVSA-N 0.000 description 27
- JDSRHVWSAMTSSN-BSZOFBHHSA-N 13-HPODE Chemical compound CCCCCC(OO)\C=C\C=C/CCCCCCCC(O)=O JDSRHVWSAMTSSN-BSZOFBHHSA-N 0.000 description 26
- 230000000875 corresponding effect Effects 0.000 description 24
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 22
- 210000001161 mammalian embryo Anatomy 0.000 description 22
- 238000012216 screening Methods 0.000 description 21
- 230000035784 germination Effects 0.000 description 20
- 239000000523 sample Substances 0.000 description 20
- 238000003556 assay Methods 0.000 description 19
- 238000011161 development Methods 0.000 description 19
- 230000018109 developmental process Effects 0.000 description 19
- 235000019634 flavors Nutrition 0.000 description 19
- 108020004999 messenger RNA Proteins 0.000 description 19
- 230000008569 process Effects 0.000 description 19
- 238000011534 incubation Methods 0.000 description 18
- 238000002703 mutagenesis Methods 0.000 description 18
- 231100000350 mutagenesis Toxicity 0.000 description 18
- 210000001519 tissue Anatomy 0.000 description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 16
- 239000004382 Amylase Substances 0.000 description 15
- 230000014509 gene expression Effects 0.000 description 15
- 238000009835 boiling Methods 0.000 description 14
- 238000001514 detection method Methods 0.000 description 14
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 14
- 239000000126 substance Substances 0.000 description 14
- 238000002474 experimental method Methods 0.000 description 13
- 108091026890 Coding region Proteins 0.000 description 12
- 229920002472 Starch Polymers 0.000 description 12
- 239000006260 foam Substances 0.000 description 12
- 238000010438 heat treatment Methods 0.000 description 12
- 230000037361 pathway Effects 0.000 description 12
- 230000009467 reduction Effects 0.000 description 12
- 235000019698 starch Nutrition 0.000 description 12
- 239000008107 starch Substances 0.000 description 12
- 230000003321 amplification Effects 0.000 description 11
- 238000003199 nucleic acid amplification method Methods 0.000 description 11
- 150000007523 nucleic acids Chemical class 0.000 description 11
- 238000002360 preparation method Methods 0.000 description 11
- 238000001035 drying Methods 0.000 description 10
- 239000000463 material Substances 0.000 description 10
- 230000002028 premature Effects 0.000 description 10
- 230000000644 propagated effect Effects 0.000 description 10
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 9
- 108091092195 Intron Proteins 0.000 description 9
- 230000002950 deficient Effects 0.000 description 9
- 230000002255 enzymatic effect Effects 0.000 description 9
- 238000001914 filtration Methods 0.000 description 9
- 108020004707 nucleic acids Proteins 0.000 description 9
- 102000039446 nucleic acids Human genes 0.000 description 9
- 235000008694 Humulus lupulus Nutrition 0.000 description 8
- 239000011521 glass Substances 0.000 description 8
- 238000004128 high performance liquid chromatography Methods 0.000 description 8
- 239000000758 substrate Substances 0.000 description 8
- 235000000346 sugar Nutrition 0.000 description 8
- 241000209094 Oryza Species 0.000 description 7
- 239000012634 fragment Substances 0.000 description 7
- 230000002068 genetic effect Effects 0.000 description 7
- 230000012010 growth Effects 0.000 description 7
- 239000004310 lactic acid Substances 0.000 description 7
- 235000014655 lactic acid Nutrition 0.000 description 7
- 239000007788 liquid Substances 0.000 description 7
- 238000000926 separation method Methods 0.000 description 7
- 238000006467 substitution reaction Methods 0.000 description 7
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 108020005067 RNA Splice Sites Proteins 0.000 description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 6
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 230000001594 aberrant effect Effects 0.000 description 6
- 230000032683 aging Effects 0.000 description 6
- 230000009418 agronomic effect Effects 0.000 description 6
- 125000000539 amino acid group Chemical group 0.000 description 6
- 230000000692 anti-sense effect Effects 0.000 description 6
- 230000008238 biochemical pathway Effects 0.000 description 6
- 239000000872 buffer Substances 0.000 description 6
- 235000009508 confectionery Nutrition 0.000 description 6
- 238000000605 extraction Methods 0.000 description 6
- 235000013305 food Nutrition 0.000 description 6
- 238000005259 measurement Methods 0.000 description 6
- 238000012986 modification Methods 0.000 description 6
- 230000004048 modification Effects 0.000 description 6
- 108091033319 polynucleotide Proteins 0.000 description 6
- 102000040430 polynucleotide Human genes 0.000 description 6
- 239000002157 polynucleotide Substances 0.000 description 6
- 230000009466 transformation Effects 0.000 description 6
- JDSRHVWSAMTSSN-IRQZEAMPSA-N 13(S)-HPODE Chemical compound CCCCC[C@H](OO)\C=C\C=C/CCCCCCCC(O)=O JDSRHVWSAMTSSN-IRQZEAMPSA-N 0.000 description 5
- 108700028369 Alleles Proteins 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 5
- 208000035240 Disease Resistance Diseases 0.000 description 5
- 229920001503 Glucan Polymers 0.000 description 5
- 235000017335 Hordeum vulgare subsp spontaneum Nutrition 0.000 description 5
- 241001299819 Hordeum vulgare subsp. spontaneum Species 0.000 description 5
- 235000007164 Oryza sativa Nutrition 0.000 description 5
- 238000009825 accumulation Methods 0.000 description 5
- 230000009471 action Effects 0.000 description 5
- 150000001299 aldehydes Chemical class 0.000 description 5
- 229940098396 barley grain Drugs 0.000 description 5
- 210000004899 c-terminal region Anatomy 0.000 description 5
- 239000003795 chemical substances by application Substances 0.000 description 5
- 150000001875 compounds Chemical class 0.000 description 5
- 201000010099 disease Diseases 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 235000013312 flour Nutrition 0.000 description 5
- 238000003801 milling Methods 0.000 description 5
- 238000004806 packaging method and process Methods 0.000 description 5
- 238000003976 plant breeding Methods 0.000 description 5
- 229920001184 polypeptide Polymers 0.000 description 5
- 108090000765 processed proteins & peptides Proteins 0.000 description 5
- 102000004196 processed proteins & peptides Human genes 0.000 description 5
- 235000009566 rice Nutrition 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 150000008163 sugars Chemical class 0.000 description 5
- 230000009261 transgenic effect Effects 0.000 description 5
- 108700024394 Exon Proteins 0.000 description 4
- 206010064571 Gene mutation Diseases 0.000 description 4
- 235000021307 Triticum Nutrition 0.000 description 4
- 241000209140 Triticum Species 0.000 description 4
- 240000008042 Zea mays Species 0.000 description 4
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 4
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 4
- 238000002835 absorbance Methods 0.000 description 4
- 239000002253 acid Substances 0.000 description 4
- 230000003466 anti-cipated effect Effects 0.000 description 4
- 239000011324 bead Substances 0.000 description 4
- -1 but not limited to Chemical compound 0.000 description 4
- 235000014633 carbohydrates Nutrition 0.000 description 4
- 150000001720 carbohydrates Chemical class 0.000 description 4
- 230000007613 environmental effect Effects 0.000 description 4
- 238000010363 gene targeting Methods 0.000 description 4
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 4
- 229910052737 gold Inorganic materials 0.000 description 4
- 239000010931 gold Substances 0.000 description 4
- 108010064894 hydroperoxide lyase Proteins 0.000 description 4
- 235000009973 maize Nutrition 0.000 description 4
- 230000007246 mechanism Effects 0.000 description 4
- 230000001404 mediated effect Effects 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 239000003471 mutagenic agent Substances 0.000 description 4
- 231100000707 mutagenic chemical Toxicity 0.000 description 4
- 230000003505 mutagenic effect Effects 0.000 description 4
- 239000012071 phase Substances 0.000 description 4
- 230000000306 recurrent effect Effects 0.000 description 4
- 241000894007 species Species 0.000 description 4
- 239000007858 starting material Substances 0.000 description 4
- 238000003786 synthesis reaction Methods 0.000 description 4
- 238000011144 upstream manufacturing Methods 0.000 description 4
- OYHQOLUKZRVURQ-NTGFUMLPSA-N (9Z,12Z)-9,10,12,13-tetratritiooctadeca-9,12-dienoic acid Chemical compound C(CCCCCCC\C(=C(/C\C(=C(/CCCCC)\[3H])\[3H])\[3H])\[3H])(=O)O OYHQOLUKZRVURQ-NTGFUMLPSA-N 0.000 description 3
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 3
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- 239000003155 DNA primer Substances 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 125000001429 N-terminal alpha-amino-acid group Chemical group 0.000 description 3
- 108700026244 Open Reading Frames Proteins 0.000 description 3
- 241000209056 Secale Species 0.000 description 3
- 238000000246 agarose gel electrophoresis Methods 0.000 description 3
- 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 description 3
- 238000012217 deletion Methods 0.000 description 3
- 230000037430 deletion Effects 0.000 description 3
- 238000001212 derivatisation Methods 0.000 description 3
- 239000000194 fatty acid Substances 0.000 description 3
- 235000019985 fermented beverage Nutrition 0.000 description 3
- JARKCYVAAOWBJS-UHFFFAOYSA-N hexanal Chemical compound CCCCCC=O JARKCYVAAOWBJS-UHFFFAOYSA-N 0.000 description 3
- 238000002188 infrared transmission spectroscopy Methods 0.000 description 3
- 239000004615 ingredient Substances 0.000 description 3
- 238000011173 large scale experimental method Methods 0.000 description 3
- 239000002207 metabolite Substances 0.000 description 3
- 239000000123 paper Substances 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- 238000010791 quenching Methods 0.000 description 3
- 239000011535 reaction buffer Substances 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- 238000002791 soaking Methods 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- 239000004458 spent grain Substances 0.000 description 3
- 239000011550 stock solution Substances 0.000 description 3
- 208000024891 symptom Diseases 0.000 description 3
- PHOLIFLKGONSGY-CSKARUKUSA-N (e)-(3-methyl-1,3-benzothiazol-2-ylidene)hydrazine Chemical compound C1=CC=C2S\C(=N\N)N(C)C2=C1 PHOLIFLKGONSGY-CSKARUKUSA-N 0.000 description 2
- ZFULIMDGARVKBJ-UHFFFAOYSA-N 18,18,18-trihydroxyoctadec-2-enoic acid Chemical class OC(CCCCCCCCCCCCCCC=CC(=O)O)(O)O ZFULIMDGARVKBJ-UHFFFAOYSA-N 0.000 description 2
- QZHXKQKKEBXYRG-UHFFFAOYSA-N 4-n-(4-aminophenyl)benzene-1,4-diamine Chemical compound C1=CC(N)=CC=C1NC1=CC=C(N)C=C1 QZHXKQKKEBXYRG-UHFFFAOYSA-N 0.000 description 2
- 244000075850 Avena orientalis Species 0.000 description 2
- 240000005220 Bischofia javanica Species 0.000 description 2
- 235000010893 Bischofia javanica Nutrition 0.000 description 2
- 108091035707 Consensus sequence Proteins 0.000 description 2
- 238000007399 DNA isolation Methods 0.000 description 2
- PLUBXMRUUVWRLT-UHFFFAOYSA-N Ethyl methanesulfonate Chemical compound CCOS(C)(=O)=O PLUBXMRUUVWRLT-UHFFFAOYSA-N 0.000 description 2
- 235000010469 Glycine max Nutrition 0.000 description 2
- 241001465807 Heliconius Species 0.000 description 2
- 102000001554 Hemoglobins Human genes 0.000 description 2
- 108010054147 Hemoglobins Proteins 0.000 description 2
- 241000282412 Homo Species 0.000 description 2
- 101000623801 Homo sapiens Protein misato homolog 1 Proteins 0.000 description 2
- 244000202745 Hordeum vulgare ssp spontaneum Species 0.000 description 2
- 235000004594 Hordeum vulgare ssp. spontaneum Nutrition 0.000 description 2
- 206010022998 Irritability Diseases 0.000 description 2
- BGRDGMRNKXEXQD-UHFFFAOYSA-N Maleic hydrazide Chemical compound OC1=CC=C(O)N=N1 BGRDGMRNKXEXQD-UHFFFAOYSA-N 0.000 description 2
- 239000005983 Maleic hydrazide Substances 0.000 description 2
- ZRKWMRDKSOPRRS-UHFFFAOYSA-N N-Methyl-N-nitrosourea Chemical compound O=NN(C)C(N)=O ZRKWMRDKSOPRRS-UHFFFAOYSA-N 0.000 description 2
- 238000000636 Northern blotting Methods 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- 229920001213 Polysorbate 20 Polymers 0.000 description 2
- 239000004365 Protease Substances 0.000 description 2
- 102100023096 Protein misato homolog 1 Human genes 0.000 description 2
- 240000006394 Sorghum bicolor Species 0.000 description 2
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 2
- WYURNTSHIVDZCO-UHFFFAOYSA-N Tetrahydrofuran Chemical compound C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 description 2
- DTQVDTLACAAQTR-UHFFFAOYSA-N Trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-N 0.000 description 2
- 108010017070 Zinc Finger Nucleases Proteins 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 2
- 230000002411 adverse Effects 0.000 description 2
- 235000013334 alcoholic beverage Nutrition 0.000 description 2
- 229940008201 allegra Drugs 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000006555 catalytic reaction Methods 0.000 description 2
- 210000002421 cell wall Anatomy 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 239000007795 chemical reaction product Substances 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 244000038559 crop plants Species 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 235000014113 dietary fatty acids Nutrition 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 239000011536 extraction buffer Substances 0.000 description 2
- 229930195729 fatty acid Natural products 0.000 description 2
- 150000004665 fatty acids Chemical class 0.000 description 2
- 230000004720 fertilization Effects 0.000 description 2
- RWTNPBWLLIMQHL-UHFFFAOYSA-N fexofenadine Chemical compound C1=CC(C(C)(C(O)=O)C)=CC=C1C(O)CCCN1CCC(C(O)(C=2C=CC=CC=2)C=2C=CC=CC=2)CC1 RWTNPBWLLIMQHL-UHFFFAOYSA-N 0.000 description 2
- 239000003205 fragrance Substances 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 210000004602 germ cell Anatomy 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 238000000265 homogenisation Methods 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 239000002198 insoluble material Substances 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 238000006317 isomerization reaction Methods 0.000 description 2
- 235000015095 lager Nutrition 0.000 description 2
- 230000000670 limiting effect Effects 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 230000010198 maturation time Effects 0.000 description 2
- 235000012054 meals Nutrition 0.000 description 2
- ZIUHHBKFKCYYJD-UHFFFAOYSA-N n,n'-methylenebisacrylamide Chemical compound C=CC(=O)NCNC(=O)C=C ZIUHHBKFKCYYJD-UHFFFAOYSA-N 0.000 description 2
- 210000004898 n-terminal fragment Anatomy 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- BSAIUMLZVGUGKX-UHFFFAOYSA-N non-2-enal Chemical compound CCCCCCC=CC=O BSAIUMLZVGUGKX-UHFFFAOYSA-N 0.000 description 2
- 235000019520 non-alcoholic beverage Nutrition 0.000 description 2
- 230000037434 nonsense mutation Effects 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- 230000035479 physiological effects, processes and functions Effects 0.000 description 2
- 238000003752 polymerase chain reaction Methods 0.000 description 2
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 2
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 2
- 235000013824 polyphenols Nutrition 0.000 description 2
- 235000020777 polyunsaturated fatty acids Nutrition 0.000 description 2
- 210000001938 protoplast Anatomy 0.000 description 2
- 239000011541 reaction mixture Substances 0.000 description 2
- 230000001850 reproductive effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000003757 reverse transcription PCR Methods 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 238000011172 small scale experimental method Methods 0.000 description 2
- 230000002269 spontaneous effect Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000013518 transcription Methods 0.000 description 2
- 230000035897 transcription Effects 0.000 description 2
- 230000007704 transition Effects 0.000 description 2
- 238000013519 translation Methods 0.000 description 2
- 239000013598 vector Substances 0.000 description 2
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 2
- DTOSIQBPPRVQHS-UHFFFAOYSA-N α-Linolenic acid Chemical compound CCC=CCC=CCC=CCCCCCCCC(O)=O DTOSIQBPPRVQHS-UHFFFAOYSA-N 0.000 description 2
- MBDOYVRWFFCFHM-SNAWJCMRSA-N (2E)-hexenal Chemical compound CCC\C=C\C=O MBDOYVRWFFCFHM-SNAWJCMRSA-N 0.000 description 1
- PMJHHCWVYXUKFD-SNAWJCMRSA-N (E)-1,3-pentadiene Chemical compound C\C=C\C=C PMJHHCWVYXUKFD-SNAWJCMRSA-N 0.000 description 1
- JYSUYJCLUODSLN-UHFFFAOYSA-N 1,3-benzothiazol-2-ylhydrazine Chemical compound C1=CC=C2SC(NN)=NC2=C1 JYSUYJCLUODSLN-UHFFFAOYSA-N 0.000 description 1
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- CEKFCZVVEZSDSK-UHFFFAOYSA-N 13-hydroperoxyoctadeca-2,4,6-trienoic acid Chemical class CCCCCC(OO)CCCCCC=CC=CC=CC(O)=O CEKFCZVVEZSDSK-UHFFFAOYSA-N 0.000 description 1
- NEGFNJRAUMCZMY-UHFFFAOYSA-N 3-(dimethylamino)benzoic acid Chemical compound CN(C)C1=CC=CC(C(O)=O)=C1 NEGFNJRAUMCZMY-UHFFFAOYSA-N 0.000 description 1
- 101150096316 5 gene Proteins 0.000 description 1
- 108010011619 6-Phytase Proteins 0.000 description 1
- 108010061073 9-hydroperoxide lyase Proteins 0.000 description 1
- XDPDIOHFEPDAFI-UHFFFAOYSA-N 9-hydroperoxyoctadeca-2,4-dienoic acid Chemical compound CCCCCCCCCC(OO)CCCC=CC=CC(O)=O XDPDIOHFEPDAFI-UHFFFAOYSA-N 0.000 description 1
- 101150001232 ALS gene Proteins 0.000 description 1
- 206010069754 Acquired gene mutation Diseases 0.000 description 1
- 241000589158 Agrobacterium Species 0.000 description 1
- 108091093088 Amplicon Proteins 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 229920000945 Amylopectin Polymers 0.000 description 1
- 108020004491 Antisense DNA Proteins 0.000 description 1
- 108020005544 Antisense RNA Proteins 0.000 description 1
- 241000219194 Arabidopsis Species 0.000 description 1
- 235000005781 Avena Nutrition 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 101710137591 C-hordein Proteins 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- 108091092236 Chimeric RNA Proteins 0.000 description 1
- 235000019750 Crude protein Nutrition 0.000 description 1
- 108010015742 Cytochrome P-450 Enzyme System Proteins 0.000 description 1
- 102000002004 Cytochrome P-450 Enzyme System Human genes 0.000 description 1
- 230000009946 DNA mutation Effects 0.000 description 1
- QSJXEFYPDANLFS-UHFFFAOYSA-N Diacetyl Chemical group CC(=O)C(C)=O QSJXEFYPDANLFS-UHFFFAOYSA-N 0.000 description 1
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 description 1
- 241000221785 Erysiphales Species 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- 108091029865 Exogenous DNA Proteins 0.000 description 1
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 1
- 108700007698 Genetic Terminator Regions Proteins 0.000 description 1
- 108010068370 Glutens Proteins 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- OAKJQQAXSVQMHS-UHFFFAOYSA-N Hydrazine Chemical compound NN OAKJQQAXSVQMHS-UHFFFAOYSA-N 0.000 description 1
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical group [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 1
- 108010044467 Isoenzymes Proteins 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 108091027974 Mature messenger RNA Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000244206 Nematoda Species 0.000 description 1
- 241000228653 Nicotiana attenuata Species 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 108091092724 Noncoding DNA Proteins 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 238000009004 PCR Kit Methods 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 241000219843 Pisum Species 0.000 description 1
- 108700001094 Plant Genes Proteins 0.000 description 1
- 241000173767 Ramularia Species 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 235000003534 Saccharomyces carlsbergensis Nutrition 0.000 description 1
- 241001123227 Saccharomyces pastorianus Species 0.000 description 1
- 241000228160 Secale cereale x Triticum aestivum Species 0.000 description 1
- 108091081021 Sense strand Proteins 0.000 description 1
- 101001086866 Sus scrofa Pulmonary surfactant-associated protein B Proteins 0.000 description 1
- 108020005038 Terminator Codon Proteins 0.000 description 1
- 108700019146 Transgenes Proteins 0.000 description 1
- 235000019714 Triticale Nutrition 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 241000209149 Zea Species 0.000 description 1
- UGXQOOQUZRUVSS-ZZXKWVIFSA-N [5-[3,5-dihydroxy-2-(1,3,4-trihydroxy-5-oxopentan-2-yl)oxyoxan-4-yl]oxy-3,4-dihydroxyoxolan-2-yl]methyl (e)-3-(4-hydroxyphenyl)prop-2-enoate Chemical compound OC1C(OC(CO)C(O)C(O)C=O)OCC(O)C1OC1C(O)C(O)C(COC(=O)\C=C\C=2C=CC(O)=CC=2)O1 UGXQOOQUZRUVSS-ZZXKWVIFSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000010306 acid treatment Methods 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 125000002252 acyl group Chemical group 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 108010050181 aleurone Proteins 0.000 description 1
- 125000003275 alpha amino acid group Chemical group 0.000 description 1
- 108090000637 alpha-Amylases Proteins 0.000 description 1
- DTOSIQBPPRVQHS-PDBXOOCHSA-N alpha-linolenic acid Chemical compound CC\C=C/C\C=C/C\C=C/CCCCCCCC(O)=O DTOSIQBPPRVQHS-PDBXOOCHSA-N 0.000 description 1
- 235000020661 alpha-linolenic acid Nutrition 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 229940025131 amylases Drugs 0.000 description 1
- 239000003816 antisense DNA Substances 0.000 description 1
- 229920000617 arabinoxylan Polymers 0.000 description 1
- 108010052439 arabinoxylanase Proteins 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 239000012131 assay buffer Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 229940067467 barley malt syrup Drugs 0.000 description 1
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid group Chemical group C(C1=CC=CC=C1)(=O)O WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 235000019658 bitter taste Nutrition 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 235000008429 bread Nutrition 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 150000001728 carbonyl compounds Chemical class 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000007248 cellular mechanism Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000002759 chromosomal effect Effects 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 239000003184 complementary RNA Substances 0.000 description 1
- 229920002770 condensed tannin Polymers 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000010154 cross-pollination Effects 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000002939 deleterious effect Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 230000000368 destabilizing effect Effects 0.000 description 1
- SWULYVBHRSPZCK-UHFFFAOYSA-N diazonio(1,2,3-trihydroxypropyl)azanide Chemical compound OCC(O)C(O)[N-][N+]#N SWULYVBHRSPZCK-UHFFFAOYSA-N 0.000 description 1
- 239000005546 dideoxynucleotide Substances 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 229910001873 dinitrogen Inorganic materials 0.000 description 1
- 229910001882 dioxygen Inorganic materials 0.000 description 1
- 235000021186 dishes Nutrition 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000003828 downregulation Effects 0.000 description 1
- 210000005069 ears Anatomy 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000004129 fatty acid metabolism Effects 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 231100000221 frame shift mutation induction Toxicity 0.000 description 1
- 230000037433 frameshift Effects 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 238000004817 gas chromatography Methods 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 230000008570 general process Effects 0.000 description 1
- 238000012252 genetic analysis Methods 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 238000010362 genome editing Methods 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 239000008241 heterogeneous mixture Substances 0.000 description 1
- 238000012203 high throughput assay Methods 0.000 description 1
- 238000013537 high throughput screening Methods 0.000 description 1
- 230000036571 hydration Effects 0.000 description 1
- 238000006703 hydration reaction Methods 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 238000005304 joining Methods 0.000 description 1
- 150000004715 keto acids Chemical class 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 229960004488 linolenic acid Drugs 0.000 description 1
- KQQKGWQCNNTQJW-UHFFFAOYSA-N linolenic acid Natural products CC=CCCC=CCC=CCCCCCCCC(O)=O KQQKGWQCNNTQJW-UHFFFAOYSA-N 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 230000000869 mutational effect Effects 0.000 description 1
- 238000002414 normal-phase solid-phase extraction Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- UOISMTPJFYEVBW-UHFFFAOYSA-N o-[(2,3,4,5,6-pentafluorophenyl)methyl]hydroxylamine Chemical compound NOCC1=C(F)C(F)=C(F)C(F)=C1F UOISMTPJFYEVBW-UHFFFAOYSA-N 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 238000005691 oxidative coupling reaction Methods 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000000137 peptide hydrolase inhibitor Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 229940085127 phytase Drugs 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 244000000003 plant pathogen Species 0.000 description 1
- 239000013600 plasmid vector Substances 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000021395 porridge Nutrition 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- ULWHHBHJGPPBCO-UHFFFAOYSA-N propane-1,1-diol Chemical compound CCC(O)O ULWHHBHJGPPBCO-UHFFFAOYSA-N 0.000 description 1
- 235000019419 proteases Nutrition 0.000 description 1
- 230000009979 protective mechanism Effects 0.000 description 1
- 230000004853 protein function Effects 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000000275 quality assurance Methods 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 238000002708 random mutagenesis Methods 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 230000021749 root development Effects 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 230000014284 seed dormancy process Effects 0.000 description 1
- 238000010187 selection method Methods 0.000 description 1
- 230000010153 self-pollination Effects 0.000 description 1
- 230000011869 shoot development Effects 0.000 description 1
- 238000002741 site-directed mutagenesis Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000037439 somatic mutation Effects 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- YLQBMQCUIZJEEH-UHFFFAOYSA-N tetrahydrofuran Natural products C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 210000003934 vacuole Anatomy 0.000 description 1
- 239000003039 volatile agent Substances 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
- C12C12/00—Processes specially adapted for making special kinds of beer
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
- A01H5/10—Seeds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
- A01H6/4624—Hordeum vulgarus [barley]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
- C12C1/00—Preparation of malt
- C12C1/18—Preparation of malt extract or of special kinds of malt, e.g. caramel, black malt
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
- C12C2200/00—Special features
- C12C2200/01—Use of specific genetic variants of barley or other sources of fermentable carbohydrates for beer brewing
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Physiology (AREA)
- Environmental Sciences (AREA)
- Microbiology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Non-Alcoholic Beverages (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Distillation Of Fermentation Liquor, Processing Of Alcohols, Vinegar And Beer (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DKPA200801851 | 2008-12-30 | ||
| PCT/DK2009/050355 WO2010075860A2 (en) | 2008-12-30 | 2009-12-28 | Barley with reduced lipoxygenase activity |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| NZ593962A true NZ593962A (en) | 2012-04-27 |
Family
ID=41153271
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NZ593962A NZ593962A (en) | 2008-12-30 | 2009-12-28 | Barley with reduced lipoxygenase activity and beverage prepared therefrom |
Country Status (17)
| Country | Link |
|---|---|
| US (1) | US9363959B2 (https=) |
| EP (1) | EP2384110B1 (https=) |
| JP (1) | JP5670347B2 (https=) |
| CN (1) | CN102333440B (https=) |
| AR (1) | AR074913A1 (https=) |
| AU (1) | AU2009335397B2 (https=) |
| BR (1) | BRPI0923863A2 (https=) |
| CA (1) | CA2747225A1 (https=) |
| EA (1) | EA028532B1 (https=) |
| MX (1) | MX2011006588A (https=) |
| MY (1) | MY155960A (https=) |
| NZ (1) | NZ593962A (https=) |
| SA (1) | SA109310019B1 (https=) |
| UA (1) | UA106597C2 (https=) |
| UY (1) | UY32372A (https=) |
| WO (1) | WO2010075860A2 (https=) |
| ZA (1) | ZA201105047B (https=) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR112012030806B8 (pt) * | 2010-06-03 | 2022-05-10 | Carlsberg Breweries As | Método para preparar uma bebida à base de cevada e composição de malte moído |
| CN103114129B (zh) * | 2012-11-08 | 2016-08-31 | 中国农业科学院作物科学研究所 | 一种大麦脂肪氧化酶(lox-1)合成缺陷基因的多态性分子标记方法 |
| CN103018388B (zh) * | 2013-01-21 | 2015-01-07 | 江苏省农业科学院 | 一种以己醛含量判别甜玉米烫漂终点的方法 |
| JP6470948B2 (ja) * | 2014-11-17 | 2019-02-13 | アサヒビール株式会社 | コク感が増強された麦芽発酵飲料 |
| JP2017127228A (ja) * | 2016-01-19 | 2017-07-27 | サッポロビール株式会社 | ノンアルコールビールテイスト飲料の製造方法 |
| JP6691826B2 (ja) * | 2016-06-01 | 2020-05-13 | サッポロビール株式会社 | 蒸留酒類 |
| KR102433243B1 (ko) | 2016-07-01 | 2022-08-17 | 칼스버그 브류어리스 에이/에스 | 정제 곡물계 음료 |
| CN106405024B (zh) * | 2016-08-25 | 2018-08-24 | 青岛啤酒股份有限公司 | 一种评价麦芽脂质氧化程度的方法 |
| MA51410A (fr) * | 2017-12-28 | 2021-04-07 | Carlsberg As | Plantes céréalières présentant des propriétés de paroi cellulaire améliorées |
| HUE066457T2 (hu) | 2017-12-28 | 2024-08-28 | Carlsberg As | Gyors módszerek gabona kivonatok készítéséhez |
| CA3086078A1 (en) | 2017-12-28 | 2019-07-04 | Carlsberg A/S | Method for producing an extract of cereal and method for processing this extract into beverage |
| CA3085899A1 (en) | 2017-12-28 | 2019-07-04 | Carlsberg A/S | Barley with increased hydrolytic enzyme activity |
| EP3784058A1 (en) | 2018-04-25 | 2021-03-03 | Carlsberg A/S | Barley based beverages |
| JP6709301B2 (ja) * | 2019-01-21 | 2020-06-10 | アサヒビール株式会社 | コク感が増強された麦芽発酵飲料 |
| JP7428504B2 (ja) * | 2019-11-15 | 2024-02-06 | サッポロビール株式会社 | 麦芽原料液又はビールテイスト飲料の製造方法、及び、麦芽原料液又はビールテイスト飲料の香味を向上させる方法 |
| MX2022010777A (es) * | 2020-03-02 | 2022-11-30 | Carlsberg As | Plantas de cebada con elevada actividad de dextrinasa limite. |
| AR132565A1 (es) | 2023-05-01 | 2025-07-16 | Carlsberg As | Cebada con propiedades mejoradas |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
| DE4428978A1 (de) | 1993-09-29 | 1995-03-30 | Weissheimer Friedr Malzfab | Verfahren zur Herstellung eines Malzproduktes für Lebensmittel, insbesondere für die Bierherstellung, und ein nach diesem Verfahren hergestelltes Malzprodukt |
| US6696294B1 (en) | 1998-06-19 | 2004-02-24 | Northwest Plant Breeding Co. | Methods for generating and identifying mutant polyploid plants, and uses therefor |
| US6660915B2 (en) * | 2000-12-29 | 2003-12-09 | Anna Christina Douma | Low lipoxygenase 1 barley |
| EP2305797A3 (en) * | 2000-12-29 | 2011-07-13 | Carlsberg Research Laboratory | Low-lipoxygenase 1 barley |
| EP1527157A1 (en) * | 2002-07-25 | 2005-05-04 | Novozymes A/S | Mashing process |
| JP4113795B2 (ja) | 2003-03-25 | 2008-07-09 | サッポロビール株式会社 | 大麦リポキシゲナーゼ−１遺伝子、大麦の選抜方法、麦芽アルコール飲料用原料及び麦芽アルコール飲料の製造方法 |
| US7420105B2 (en) * | 2004-03-11 | 2008-09-02 | Carlsberg A/S | Barley for production of flavor-stable beverage |
| US20060005276A1 (en) * | 2004-03-12 | 2006-01-05 | Falco Saverio C | Transgenic soybean seeds having reduced activity of lipoxygenases |
| JP2006034129A (ja) | 2004-07-23 | 2006-02-09 | Asahi Breweries Ltd | 麦芽の製造方法 |
| BRPI0615947B1 (pt) * | 2005-09-07 | 2023-12-19 | Monsanto Technology Llc | Método de prognosticar o fenótipo de uma planta de soja para teor de glicinina e de beta-conglicinina, e kit |
-
2009
- 2009-12-26 SA SA109310019A patent/SA109310019B1/ar unknown
- 2009-12-28 BR BRPI0923863-8A patent/BRPI0923863A2/pt not_active Application Discontinuation
- 2009-12-28 AU AU2009335397A patent/AU2009335397B2/en not_active Ceased
- 2009-12-28 MX MX2011006588A patent/MX2011006588A/es active IP Right Grant
- 2009-12-28 US US13/141,579 patent/US9363959B2/en not_active Expired - Fee Related
- 2009-12-28 AR ARP090105124A patent/AR074913A1/es not_active Application Discontinuation
- 2009-12-28 WO PCT/DK2009/050355 patent/WO2010075860A2/en not_active Ceased
- 2009-12-28 EP EP09801148.9A patent/EP2384110B1/en not_active Revoked
- 2009-12-28 JP JP2011542680A patent/JP5670347B2/ja not_active Expired - Fee Related
- 2009-12-28 NZ NZ593962A patent/NZ593962A/en not_active IP Right Cessation
- 2009-12-28 EA EA201170906A patent/EA028532B1/ru not_active IP Right Cessation
- 2009-12-28 CN CN200980157631.1A patent/CN102333440B/zh not_active Expired - Fee Related
- 2009-12-28 UA UAA201109384A patent/UA106597C2/uk unknown
- 2009-12-28 CA CA2747225A patent/CA2747225A1/en not_active Abandoned
- 2009-12-28 MY MYPI2011003031A patent/MY155960A/en unknown
- 2009-12-29 UY UY0001032372A patent/UY32372A/es not_active Application Discontinuation
-
2011
- 2011-07-08 ZA ZA2011/05047A patent/ZA201105047B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| ZA201105047B (en) | 2012-10-31 |
| CN102333440A (zh) | 2012-01-25 |
| AR074913A1 (es) | 2011-02-23 |
| SA109310019B1 (ar) | 2014-09-10 |
| JP2012513744A (ja) | 2012-06-21 |
| US9363959B2 (en) | 2016-06-14 |
| EA028532B1 (ru) | 2017-11-30 |
| UA106597C2 (uk) | 2014-09-25 |
| EP2384110B1 (en) | 2016-04-20 |
| WO2010075860A3 (en) | 2011-07-14 |
| CA2747225A1 (en) | 2010-07-08 |
| UY32372A (es) | 2010-02-26 |
| BRPI0923863A2 (pt) | 2020-08-11 |
| WO2010075860A2 (en) | 2010-07-08 |
| EA201170906A1 (ru) | 2012-02-28 |
| US20110318469A1 (en) | 2011-12-29 |
| MY155960A (en) | 2015-12-31 |
| HK1162851A1 (zh) | 2012-09-07 |
| AU2009335397A1 (en) | 2011-07-28 |
| JP5670347B2 (ja) | 2015-02-18 |
| MX2011006588A (es) | 2011-09-27 |
| AU2009335397A8 (en) | 2011-08-04 |
| CN102333440B (zh) | 2014-07-23 |
| EP2384110A2 (en) | 2011-11-09 |
| AU2009335397B2 (en) | 2014-08-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US9363959B2 (en) | Barley with reduced lipoxygenase activity and beverage prepared therefrom | |
| US20190078042A1 (en) | Barley for production of flavor-stable beverage | |
| CA2801142C (en) | Energy saving brewing method | |
| US20170183611A1 (en) | Energy saving brewing method | |
| LI et al. | GERSTE MIT REDUZIERTER LIPOXYGENASE AKTIVITÄT UND EIN DAMIT HERGESTELLTES GETRÄNK ORGE À ACTIVITÉ RÉDUITE EN LIPOXYGÉNASE ET UNE BOISSON PRODUITE À PARTIR DE CETTE ORGE | |
| HK1162851B (en) | Barley with reduced lipoxygenase activity and beverage prepared therefrom |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RENW | Renewal (renewal fees accepted) |
Free format text: PATENT RENEWED FOR 3 YEARS UNTIL 28 DEC 2016 BY FB RICE Effective date: 20131128 |
|
| RENW | Renewal (renewal fees accepted) |
Free format text: PATENT RENEWED FOR 1 YEAR UNTIL 28 DEC 2017 BY ANETTE NISSEN Effective date: 20161118 |
|
| RENW | Renewal (renewal fees accepted) |
Free format text: PATENT RENEWED FOR 1 YEAR UNTIL 28 DEC 2018 BY ANETTE NISSEN Effective date: 20171202 |
|
| RENW | Renewal (renewal fees accepted) |
Free format text: PATENT RENEWED FOR 1 YEAR UNTIL 28 DEC 2019 BY ANETTE NISSEN Effective date: 20181204 |
|
| RENW | Renewal (renewal fees accepted) |
Free format text: PATENT RENEWED FOR 1 YEAR UNTIL 28 DEC 2020 BY ANETTE NISSEN Effective date: 20191127 |
|
| LAPS | Patent lapsed |