NL2034093B1 - Method for separating exosomes from apostichopus japonicus body fluid sample - Google Patents

Method for separating exosomes from apostichopus japonicus body fluid sample Download PDF

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Publication number
NL2034093B1
NL2034093B1 NL2034093A NL2034093A NL2034093B1 NL 2034093 B1 NL2034093 B1 NL 2034093B1 NL 2034093 A NL2034093 A NL 2034093A NL 2034093 A NL2034093 A NL 2034093A NL 2034093 B1 NL2034093 B1 NL 2034093B1
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Prior art keywords
apostichopus japonicus
exosomes
fluid sample
body fluid
isotonic
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NL2034093A
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English (en)
Dutch (nl)
Inventor
Liu Shilin
Lin Chenggang
Yang Hongsheng
Sun Lina
Cui Wei
Huo Da
Su Fang
Zhang Libin
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Inst Oceanology Cas
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Publication of NL2034093B1 publication Critical patent/NL2034093B1/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
    • B04B5/0414—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D36/00—Filter circuits or combinations of filters with other separating devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/10—Centrifuges combined with other apparatus, e.g. electrostatic separators; Sets or systems of several centrifuges
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q

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  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
NL2034093A 2022-03-17 2023-02-06 Method for separating exosomes from apostichopus japonicus body fluid sample NL2034093B1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202210264562.1A CN114700186B (zh) 2022-03-17 2022-03-17 一种刺参体液样品外泌体分离的方法

Publications (1)

Publication Number Publication Date
NL2034093B1 true NL2034093B1 (en) 2023-09-29

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ID=82168268

Family Applications (1)

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NL2034093A NL2034093B1 (en) 2022-03-17 2023-02-06 Method for separating exosomes from apostichopus japonicus body fluid sample

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CN (1) CN114700186B (zh)
NL (1) NL2034093B1 (zh)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116034992B (zh) * 2023-03-08 2023-06-27 中国科学院海洋研究所 刺参精子低温保存液及应用和刺参精子的保存方法

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CN108802374A (zh) * 2018-06-25 2018-11-13 中山大学附属第五医院 基于磁富集电化学发光的外泌体核酸检测技术
CN112626002A (zh) * 2019-09-24 2021-04-09 深圳光彩生命工程技术有限公司 一种分离和纯化外泌体的方法
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JP6818346B2 (ja) * 2016-04-28 2021-01-20 国立大学法人浜松医科大学 電子顕微鏡によるナノ粒子の直接的な同定・定量のための検出キットおよび方法
CN106124282B (zh) * 2016-07-26 2019-07-16 广州海力特生物科技有限公司 一种叠层离心过滤分离提取外泌体的方法
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CN111257073B (zh) * 2020-01-19 2022-03-25 北京尧景基因技术有限公司 一种用于分离临床样本中的糖基化外泌体的凝集素-大分子载体偶联复合物
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Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107153023A (zh) * 2016-03-02 2017-09-12 上海润腾生物科技有限公司 一种分离尿液中外泌体的方法
CN108802374A (zh) * 2018-06-25 2018-11-13 中山大学附属第五医院 基于磁富集电化学发光的外泌体核酸检测技术
CN112626002A (zh) * 2019-09-24 2021-04-09 深圳光彩生命工程技术有限公司 一种分离和纯化外泌体的方法
RU2750928C1 (ru) * 2020-06-03 2021-07-06 Федеральное государственное бюджетное образовательное учреждение высшего образования "Амурская государственный медицинская академия" Министерства здравоохранения Российской Федерации Способ выделения экзосом из кондиционированной среды культуры клеток

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CN114700186A (zh) 2022-07-05
CN114700186B (zh) 2023-04-18

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