MX382334B - Métodos para producir glucoproteínas recombinantes con glucosilación modificada. - Google Patents

Métodos para producir glucoproteínas recombinantes con glucosilación modificada.

Info

Publication number
MX382334B
MX382334B MX2016012085A MX2016012085A MX382334B MX 382334 B MX382334 B MX 382334B MX 2016012085 A MX2016012085 A MX 2016012085A MX 2016012085 A MX2016012085 A MX 2016012085A MX 382334 B MX382334 B MX 382334B
Authority
MX
Mexico
Prior art keywords
methods
modified glycosylation
producing recombinant
recombinant glycoproteins
animal cells
Prior art date
Application number
MX2016012085A
Other languages
English (en)
Other versions
MX2016012085A (es
Inventor
Che- Haorz Wu
Hung- Chi Chen
Nien-Yi Chen
Winston Town
Original Assignee
Fountain Biopharma Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fountain Biopharma Inc filed Critical Fountain Biopharma Inc
Publication of MX2016012085A publication Critical patent/MX2016012085A/es
Publication of MX382334B publication Critical patent/MX382334B/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2887—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against CD20
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/42—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against immunoglobulins
    • C07K16/4283—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against immunoglobulins against an allotypic or isotypic determinant on Ig
    • C07K16/4291—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against immunoglobulins against an allotypic or isotypic determinant on Ig against IgE
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01051—Alpha-L-fucosidase (3.2.1.51)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01096—Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (3.2.1.96)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41—Glycosylation, sialylation, or fucosylation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La presente invención se refiere accélulas animales hospederas diseñadas genéticamente capaces de producir glucoproteínas que tienen patrones de glucosilación modificados, por ejemplo, defucosilación y/o monoglucosilación; tales células animales hospederas se pueden diseñar para expresar fucosidasa, endoglucosidasa o ambas.
MX2016012085A 2014-03-17 2015-03-17 Métodos para producir glucoproteínas recombinantes con glucosilación modificada. MX382334B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461954337P 2014-03-17 2014-03-17
PCT/IB2015/001203 WO2015145268A2 (en) 2014-03-17 2015-03-17 Methods for producing recombinant glycoproteins with modified glycosylation

Publications (2)

Publication Number Publication Date
MX2016012085A MX2016012085A (es) 2017-05-23
MX382334B true MX382334B (es) 2025-03-13

Family

ID=54068262

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2016012085A MX382334B (es) 2014-03-17 2015-03-17 Métodos para producir glucoproteínas recombinantes con glucosilación modificada.

Country Status (19)

Country Link
US (2) US9540673B2 (es)
EP (1) EP3119882B1 (es)
JP (2) JP6701089B2 (es)
KR (1) KR102333923B1 (es)
CN (1) CN106715686B (es)
AU (1) AU2015237951B2 (es)
BR (1) BR112016021284A2 (es)
CA (1) CA2942618A1 (es)
DK (1) DK3119882T3 (es)
ES (1) ES2904993T3 (es)
HU (1) HUE057904T2 (es)
IL (1) IL247776B (es)
MX (1) MX382334B (es)
PL (1) PL3119882T3 (es)
PT (1) PT3119882T (es)
RU (1) RU2704830C2 (es)
SG (1) SG11201607759RA (es)
TW (1) TWI682033B (es)
WO (1) WO2015145268A2 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3904388A1 (en) * 2014-05-27 2021-11-03 Academia Sinica Fucosidase from bacteroides and methods using the same
CN114026229B (zh) * 2019-08-05 2025-03-25 醣基生医股份有限公司 用于重塑抗体醣型之融合蛋白
CN110885809B (zh) * 2019-12-04 2021-08-17 中国农业大学 α-L-岩藻糖苷酶及其相关生物材料与应用
AU2022289365A1 (en) 2021-06-07 2023-12-14 Amgen Inc. Using fucosidase to control afucosylation level of glycosylated proteins

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2434961T5 (es) * 1998-04-20 2018-01-18 Roche Glycart Ag Ingeniería de glicosilación de anticuerpos para mejorar la citotoxicidad celular dependiente del anticuerpo
CA2655246A1 (en) * 2006-06-09 2007-12-21 University Of Maryland, Baltimore Glycosylation engineered antibody therapy
CN101466402A (zh) * 2006-06-09 2009-06-24 马里兰卅大学(巴尔的摩) 糖基化改造的抗体治疗
GB0624874D0 (en) * 2006-12-13 2007-01-24 Hansa Medical Ab Treatment
RU2479629C2 (ru) * 2007-03-07 2013-04-20 Гликофи, Инк. Продукция гликопротеинов с модифицированным фукозилированием
KR101054362B1 (ko) * 2008-07-03 2011-08-05 재단법인 목암생명공학연구소 재조합 단백질의 푸코스 함량을 감소시키는 방법
WO2010036443A1 (en) * 2008-09-26 2010-04-01 Eureka Therapeutics, Inc. Cell lines and proteins with variant glycosylation pattern
GB0821100D0 (en) * 2008-11-18 2008-12-24 Hansa Medical Ab Antibodies
CA2751730A1 (en) * 2009-02-25 2010-09-02 Merck Sharp & Dohme Corp. Metabolic engineering of a galactose assimilation pathway in the glycoengineered yeast pichia pastoris
ES2528321T3 (es) * 2009-10-02 2015-02-06 Roche Glycart Ag Detección de a-fucosilación en anticuerpos
US10087236B2 (en) * 2009-12-02 2018-10-02 Academia Sinica Methods for modifying human antibodies by glycan engineering
US9434786B2 (en) * 2012-02-10 2016-09-06 University Of Maryland, Baltimore Chemoenzymatic glycoengineering of antibodies and Fc fragments thereof

Also Published As

Publication number Publication date
US10745729B2 (en) 2020-08-18
DK3119882T3 (da) 2022-01-31
CA2942618A1 (en) 2015-10-01
EP3119882B1 (en) 2021-11-17
PL3119882T3 (pl) 2022-05-16
BR112016021284A2 (pt) 2017-10-03
WO2015145268A2 (en) 2015-10-01
US20150259720A1 (en) 2015-09-17
TW201623620A (zh) 2016-07-01
IL247776B (en) 2021-05-31
CN106715686B (zh) 2021-03-16
AU2015237951B2 (en) 2021-07-01
TWI682033B (zh) 2020-01-11
HUE057904T2 (hu) 2022-06-28
EP3119882A2 (en) 2017-01-25
KR20160127830A (ko) 2016-11-04
RU2704830C2 (ru) 2019-10-31
JP2017507664A (ja) 2017-03-23
US9540673B2 (en) 2017-01-10
WO2015145268A3 (en) 2016-01-21
JP2020072667A (ja) 2020-05-14
US20170145463A1 (en) 2017-05-25
ES2904993T3 (es) 2022-04-06
SG11201607759RA (en) 2016-10-28
KR102333923B1 (ko) 2021-12-02
MX2016012085A (es) 2017-05-23
IL247776A0 (en) 2016-11-30
PT3119882T (pt) 2022-01-20
EP3119882A4 (en) 2017-08-16
RU2016140528A3 (es) 2018-10-18
JP6701089B2 (ja) 2020-05-27
WO2015145268A8 (en) 2015-12-03
CN106715686A (zh) 2017-05-24
AU2015237951A1 (en) 2016-10-20
RU2016140528A (ru) 2018-04-17

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