BR112016021284A2 - Processos para produção de glicoproteínas recombinantes com glicosilação modificada - Google Patents

Processos para produção de glicoproteínas recombinantes com glicosilação modificada

Info

Publication number
BR112016021284A2
BR112016021284A2 BR112016021284A BR112016021284A BR112016021284A2 BR 112016021284 A2 BR112016021284 A2 BR 112016021284A2 BR 112016021284 A BR112016021284 A BR 112016021284A BR 112016021284 A BR112016021284 A BR 112016021284A BR 112016021284 A2 BR112016021284 A2 BR 112016021284A2
Authority
BR
Brazil
Prior art keywords
processes
production
modified glycosylation
recombinant glycoproteins
modified
Prior art date
Application number
BR112016021284A
Other languages
English (en)
Inventor
Chen Nien-Yi
Wu Che-Haorz
Chen Hung-Chi
Town Winston
Original Assignee
Fountain Biopharma Inc
Oneness Biotech Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fountain Biopharma Inc, Oneness Biotech Co Ltd filed Critical Fountain Biopharma Inc
Publication of BR112016021284A2 publication Critical patent/BR112016021284A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2887—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against CD20
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/42—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against immunoglobulins
    • C07K16/4283—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against immunoglobulins against an allotypic or isotypic determinant on Ig
    • C07K16/4291—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against immunoglobulins against an allotypic or isotypic determinant on Ig against IgE
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01051—Alpha-L-fucosidase (3.2.1.51)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01096—Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (3.2.1.96)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41—Glycosylation, sialylation, or fucosylation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

PROCESSOS PARA PRODUÇÃO DE GLICOPROTEÍNAS RECOMBINANTES COM GLICOSILAÇÃO MODIFICADA. A presente invenção refere-se a células animais hospedeiras construídas geneticamente tendo padrões de glicosilação modificados, por exemplo, desfucosilação e/ou monoglicosilação. Tais células animais hospedeiras podem ser construídas para expressão de fucosidase, endoglicosidase ou ambas.
BR112016021284A 2014-03-17 2015-03-17 Processos para produção de glicoproteínas recombinantes com glicosilação modificada BR112016021284A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461954337P 2014-03-17 2014-03-17
PCT/IB2015/001203 WO2015145268A2 (en) 2014-03-17 2015-03-17 Methods for producing recombinant glycoproteins with modified glycosylation

Publications (1)

Publication Number Publication Date
BR112016021284A2 true BR112016021284A2 (pt) 2017-10-03

Family

ID=54068262

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112016021284A BR112016021284A2 (pt) 2014-03-17 2015-03-17 Processos para produção de glicoproteínas recombinantes com glicosilação modificada

Country Status (19)

Country Link
US (2) US9540673B2 (pt)
EP (1) EP3119882B1 (pt)
JP (2) JP6701089B2 (pt)
KR (1) KR102333923B1 (pt)
CN (1) CN106715686B (pt)
AU (1) AU2015237951B2 (pt)
BR (1) BR112016021284A2 (pt)
CA (1) CA2942618A1 (pt)
DK (1) DK3119882T3 (pt)
ES (1) ES2904993T3 (pt)
HU (1) HUE057904T2 (pt)
IL (1) IL247776B (pt)
MX (1) MX2016012085A (pt)
PL (1) PL3119882T3 (pt)
PT (1) PT3119882T (pt)
RU (1) RU2704830C2 (pt)
SG (1) SG11201607759RA (pt)
TW (1) TWI682033B (pt)
WO (1) WO2015145268A2 (pt)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102576850B1 (ko) * 2014-05-27 2023-09-11 아카데미아 시니카 박테로이드 기원의 푸코시다제 및 이의 사용 방법
AU2020325204B2 (en) * 2019-08-05 2024-01-04 Cho Pharma, Inc. Fusion protein for remodeling antibody glycoform
CN110885809B (zh) * 2019-12-04 2021-08-17 中国农业大学 α-L-岩藻糖苷酶及其相关生物材料与应用
AU2022289365A1 (en) 2021-06-07 2023-12-14 Amgen Inc. Using fucosidase to control afucosylation level of glycosylated proteins

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999054342A1 (en) 1998-04-20 1999-10-28 Pablo Umana Glycosylation engineering of antibodies for improving antibody-dependent cellular cytotoxicity
WO2007146847A2 (en) * 2006-06-09 2007-12-21 University Of Maryland, Baltimore Glycosylation engineered antibody therapy
CN101466402A (zh) * 2006-06-09 2009-06-24 马里兰卅大学(巴尔的摩) 糖基化改造的抗体治疗
GB0624874D0 (en) * 2006-12-13 2007-01-24 Hansa Medical Ab Treatment
EP2134834B1 (en) * 2007-03-07 2013-04-17 GlycoFi, Inc. Production of glycoproteins with modified fucosylation
KR101054362B1 (ko) * 2008-07-03 2011-08-05 재단법인 목암생명공학연구소 재조합 단백질의 푸코스 함량을 감소시키는 방법
JP2012503656A (ja) * 2008-09-26 2012-02-09 エウレカ セラピューティクス，インコーポレイテッド 変異体グリコシル化パターンを有する細胞株およびタンパク質
GB0821100D0 (en) * 2008-11-18 2008-12-24 Hansa Medical Ab Antibodies
US20120003695A1 (en) * 2009-02-25 2012-01-05 Davidson Robert C Metabolic engineering of a galactose assimilation pathway in the glycoengineered yeast pichia pastoris
US20120196310A1 (en) * 2009-10-02 2012-08-02 Christiane Jaeger A-fucosylation detection in antibodies
US10087236B2 (en) * 2009-12-02 2018-10-02 Academia Sinica Methods for modifying human antibodies by glycan engineering
CN104220603B (zh) * 2012-02-10 2017-06-06 马里兰大学，巴尔的摩 抗体及其Fc片段的化学酶法糖基化工程

Also Published As

Publication number Publication date
IL247776A0 (en) 2016-11-30
WO2015145268A3 (en) 2016-01-21
DK3119882T3 (da) 2022-01-31
US20170145463A1 (en) 2017-05-25
ES2904993T3 (es) 2022-04-06
IL247776B (en) 2021-05-31
EP3119882A2 (en) 2017-01-25
KR20160127830A (ko) 2016-11-04
AU2015237951B2 (en) 2021-07-01
WO2015145268A2 (en) 2015-10-01
EP3119882B1 (en) 2021-11-17
WO2015145268A8 (en) 2015-12-03
US20150259720A1 (en) 2015-09-17
JP6701089B2 (ja) 2020-05-27
US9540673B2 (en) 2017-01-10
JP2020072667A (ja) 2020-05-14
RU2016140528A (ru) 2018-04-17
JP2017507664A (ja) 2017-03-23
PT3119882T (pt) 2022-01-20
CA2942618A1 (en) 2015-10-01
KR102333923B1 (ko) 2021-12-02
CN106715686B (zh) 2021-03-16
RU2704830C2 (ru) 2019-10-31
TW201623620A (zh) 2016-07-01
AU2015237951A1 (en) 2016-10-20
PL3119882T3 (pl) 2022-05-16
CN106715686A (zh) 2017-05-24
RU2016140528A3 (pt) 2018-10-18
EP3119882A4 (en) 2017-08-16
HUE057904T2 (hu) 2022-06-28
MX2016012085A (es) 2017-05-23
TWI682033B (zh) 2020-01-11
SG11201607759RA (en) 2016-10-28
US10745729B2 (en) 2020-08-18

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Legal Events

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B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]

Free format text: DE ACORDO COM O ARTIGO 229-C DA LEI NO 10196/2001, QUE MODIFICOU A LEI NO 9279/96, A CONCESSAO DA PATENTE ESTA CONDICIONADA A ANUENCIA PREVIA DA ANVISA. CONSIDERANDO A APROVACAO DOS TERMOS DO PARECER NO 337/PGF/EA/2010, BEM COMO A PORTARIA INTERMINISTERIAL NO 1065 DE 24/05/2012, ENCAMINHA-SE O PRESENTE PEDIDO PARA AS PROVIDENCIAS CABIVEIS.

B350 Update of information on the portal [chapter 15.35 patent gazette]
B07G Grant request does not fulfill article 229-c lpi (prior consent of anvisa) [chapter 7.7 patent gazette]

Free format text: NOTIFICACAO DE DEVOLUCAO DO PEDIDO EM FUNCAO DA REVOGACAO DO ART. 229-C DA LEI NO 9.279, DE 1996, POR FORCA DA LEI NO 14.195, DE 2021

B25A Requested transfer of rights approved

Owner name: ONENESS BIOTECH CO., LTD. (CN)

B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements