MX375420B - Ingenieria genetica multiplexada habilitada por crispr. - Google Patents

Ingenieria genetica multiplexada habilitada por crispr.

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Publication number
MX375420B
MX375420B MX2016010285A MX2016010285A MX375420B MX 375420 B MX375420 B MX 375420B MX 2016010285 A MX2016010285 A MX 2016010285A MX 2016010285 A MX2016010285 A MX 2016010285A MX 375420 B MX375420 B MX 375420B
Authority
MX
Mexico
Prior art keywords
crispr
genetic engineering
multiplexed genetic
enabled
enabled multiplexed
Prior art date
Application number
MX2016010285A
Other languages
English (en)
Other versions
MX2016010285A (es
Inventor
Andrew Garst
Ryan T Gill
Original Assignee
Univ Colorado Regents
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Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=53800598&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX375420(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Univ Colorado Regents filed Critical Univ Colorado Regents
Publication of MX2016010285A publication Critical patent/MX2016010285A/es
Publication of MX375420B publication Critical patent/MX375420B/es

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1024—In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
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    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/11—Applications; Uses in screening processes for the determination of target sites, i.e. of active nucleic acids
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    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/12—Applications; Uses in screening processes in functional genomics, i.e. for the determination of gene function
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    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised
    • C12N2330/31—Libraries, arrays

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
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  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
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  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)

Abstract

Se describen aquí métodos y vectores para la manipulación multiplexada, racional de cromosomas dentro de marcos de lectura abierta (por ejemplo, en bibliotecas de proteína) o cualquier segmento de un cromosoma en una célula o población de células, en el cual son usadas variaciones de sistemas CRISPR.
MX2016010285A 2014-02-11 2015-02-11 Ingenieria genetica multiplexada habilitada por crispr. MX375420B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461938608P 2014-02-11 2014-02-11
PCT/US2015/015476 WO2015123339A1 (en) 2014-02-11 2015-02-11 Crispr enabled multiplexed genome engineering

Publications (2)

Publication Number Publication Date
MX2016010285A MX2016010285A (es) 2017-01-11
MX375420B true MX375420B (es) 2025-03-06

Family

ID=53800598

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2016010285A MX375420B (es) 2014-02-11 2015-02-11 Ingenieria genetica multiplexada habilitada por crispr.

Country Status (23)

Country Link
US (16) US10266849B2 (es)
EP (4) EP3105328B1 (es)
JP (3) JP2017505145A (es)
KR (4) KR20220142547A (es)
CN (2) CN106164271B (es)
AU (2) AU2015217208B2 (es)
CA (3) CA3161903C (es)
CY (1) CY1123115T1 (es)
DE (1) DE212015000061U1 (es)
DK (1) DK3105328T3 (es)
ES (2) ES2974229T3 (es)
GB (1) GB2544382B (es)
HR (1) HRP20201040T1 (es)
HU (2) HUE049634T2 (es)
IL (2) IL246951B (es)
LT (1) LT3105328T (es)
MX (1) MX375420B (es)
PL (1) PL3105328T3 (es)
PT (1) PT3105328T (es)
RS (1) RS60492B1 (es)
SI (1) SI3105328T1 (es)
SM (1) SMT202000357T1 (es)
WO (1) WO2015123339A1 (es)

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