MX360779B - Composiciones de cultivo celular con antioxidantes y métodos para la producción de polipéptidos. - Google Patents

Composiciones de cultivo celular con antioxidantes y métodos para la producción de polipéptidos.

Info

Publication number
MX360779B
MX360779B MX2015012875A MX2015012875A MX360779B MX 360779 B MX360779 B MX 360779B MX 2015012875 A MX2015012875 A MX 2015012875A MX 2015012875 A MX2015012875 A MX 2015012875A MX 360779 B MX360779 B MX 360779B
Authority
MX
Mexico
Prior art keywords
cell culture
culture medium
cell
recombinant antibody
antioxidants
Prior art date
Application number
MX2015012875A
Other languages
English (en)
Other versions
MX2015012875A (es
Inventor
J Meier Steven
Vijayasankaran Natarajan
Varma Sharat
Yang Yi
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=50639996&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX360779(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of MX2015012875A publication Critical patent/MX2015012875A/es
Publication of MX360779B publication Critical patent/MX360779B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C12N2500/33—Amino acids other than alpha-amino carboxylic acids, e.g. beta-amino acids, taurine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/44—Thiols, e.g. mercaptoethanol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/46—Amines, e.g. putrescine

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La presente invención se refiere a un método para producir una composición de anticuerpos recombinantes con intensidad de color reducida, que comprende las siguientes etapas: cultivar una célula que comprende un ácido nucleico que codifica el anticuerpo recombinante en un medio de cultivo celular, en donde el medio de cultivo celular comprende uno o más de los componentes (a) - (d): (a) hipotaurina, (b) s-carboximetilcisteína, (c) hidroxianisol butilado, y (d) hidrato de quercitrina; y producir el anticuerpo recombinante; en donde el medio de cultivo celular que comprende uno o más de los componentes (a) - (d) reduce la intensidad de color de una composición que comprende el anticuerpo recombinante producido por la célula en comparación con una composición que comprende el anticuerpo recombinante producido por la célula cultivada en un medio de cultivo celular que no comprende uno o más de los componentes (a) - (d).
MX2015012875A 2013-03-15 2014-03-14 Composiciones de cultivo celular con antioxidantes y métodos para la producción de polipéptidos. MX360779B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361799602P 2013-03-15 2013-03-15
PCT/US2014/029772 WO2014145098A1 (en) 2013-03-15 2014-03-14 Cell culture compositions with antioxidants and methods for polypeptide production

Publications (2)

Publication Number Publication Date
MX2015012875A MX2015012875A (es) 2016-02-03
MX360779B true MX360779B (es) 2018-11-16

Family

ID=50639996

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2015012875A MX360779B (es) 2013-03-15 2014-03-14 Composiciones de cultivo celular con antioxidantes y métodos para la producción de polipéptidos.
MX2018014043A MX2018014043A (es) 2013-03-15 2015-09-14 Composiciones de cultivo celular con antioxidantes y metodos para la produccion de polipeptidos.

Family Applications After (1)

Application Number Title Priority Date Filing Date
MX2018014043A MX2018014043A (es) 2013-03-15 2015-09-14 Composiciones de cultivo celular con antioxidantes y metodos para la produccion de polipeptidos.

Country Status (18)

Country Link
US (7) US10017732B2 (es)
EP (2) EP2970875B2 (es)
JP (2) JP6591395B2 (es)
KR (2) KR102235452B1 (es)
CN (2) CN110951670A (es)
AU (3) AU2014233393B2 (es)
BR (1) BR112015021993A8 (es)
CA (1) CA2903596C (es)
ES (1) ES2798307T5 (es)
HR (1) HRP20200893T4 (es)
IL (2) IL240644B (es)
MX (2) MX360779B (es)
PL (1) PL2970875T5 (es)
RU (2) RU2020111947A (es)
SG (3) SG10201809452RA (es)
SI (1) SI2970875T2 (es)
WO (1) WO2014145098A1 (es)
ZA (2) ZA201506155B (es)

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