MX349661B - Metodo para la deteccion de una molecula de union de un enlazador multiespecifico. - Google Patents

Metodo para la deteccion de una molecula de union de un enlazador multiespecifico.

Info

Publication number
MX349661B
MX349661B MX2014008762A MX2014008762A MX349661B MX 349661 B MX349661 B MX 349661B MX 2014008762 A MX2014008762 A MX 2014008762A MX 2014008762 A MX2014008762 A MX 2014008762A MX 349661 B MX349661 B MX 349661B
Authority
MX
Mexico
Prior art keywords
detection
antibody
binding partner
multispecific binder
multispecific
Prior art date
Application number
MX2014008762A
Other languages
English (en)
Other versions
MX2014008762A (es
Inventor
Gunnar Stubenrauch Kay
Wessels Uwe
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of MX2014008762A publication Critical patent/MX2014008762A/es
Publication of MX349661B publication Critical patent/MX349661B/es

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La presente invención se refiere a un método para la determinación de la presencia y/o la cantidad de un antígeno de un anticuerpo biespecífico en una muestra, por lo que el antígeno que ha de ser detectado puede ser unido específicamente por una primera especificidad de unión del anticuerpo biespecífico, y por lo que el antígeno forma complejo con el anticuerpo biespecífico (complejo antígeno-anticuerpo biespecífico), caracterizado porque comprende los pasos de: incubar una muestra que comprende el antígeno y el anticuerpo biespecífico con un anticuerpo anti-idiotípico, que se une específicamente a la segunda especificidad de unión del anticuerpo biespecífico, que es diferente de la primera especificidad de unión, por lo que el anticuerpo anti-idiotípico se une a una fase sólida, y detectar el complejo de anticuerpo antígeno-biespecífico anticuerpo anti-idiotípico y por lo tanto determinar la presencia y/o la cantidad del antígeno forma complejo de un anticuerpo biespecífico.
MX2014008762A 2012-02-01 2013-01-29 Metodo para la deteccion de una molecula de union de un enlazador multiespecifico. MX349661B (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12153457 2012-02-01
EP12182505 2012-08-31
PCT/EP2013/051604 WO2013113663A1 (en) 2012-02-01 2013-01-29 Method for the detection of a binding partner of a multispecific binder

Publications (2)

Publication Number Publication Date
MX2014008762A MX2014008762A (es) 2014-08-27
MX349661B true MX349661B (es) 2017-08-08

Family

ID=47624068

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2014008762A MX349661B (es) 2012-02-01 2013-01-29 Metodo para la deteccion de una molecula de union de un enlazador multiespecifico.

Country Status (11)

Country Link
US (3) US9945869B2 (es)
EP (1) EP2810074B1 (es)
JP (3) JP2015507193A (es)
KR (1) KR20140128314A (es)
CN (2) CN106443016B (es)
BR (1) BR112014017630A8 (es)
CA (1) CA2859268A1 (es)
HK (1) HK1202921A1 (es)
MX (1) MX349661B (es)
RU (1) RU2620563C2 (es)
WO (1) WO2013113663A1 (es)

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RU2729371C1 (ru) 2015-10-02 2020-08-06 Ф. Хоффманн-Ля Рош Аг Биспецифические антитела, специфические к pd1 и tim3
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KR102346336B1 (ko) 2017-04-05 2022-01-04 에프. 호프만-라 로슈 아게 Pd1 및 lag3에 특이적으로 결합하는 이중특이적 항체
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WO2022065052A1 (ja) * 2020-09-24 2022-03-31 株式会社Ｊｖｃケンウッド 捕捉用抗体、固相担体、検出用抗体、固相担体粒子、並びに検出対象物質の測定キット及び測定方法
CN117805397A (zh) * 2024-02-29 2024-04-02 军科正源（北京）药物研究有限责任公司 检测游离vegf的方法

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Also Published As

Publication number Publication date
RU2620563C2 (ru) 2017-05-26
JP2017223710A (ja) 2017-12-21
CN104105966A (zh) 2014-10-15
RU2014133701A (ru) 2016-03-27
US20210055304A1 (en) 2021-02-25
CN106443016A (zh) 2017-02-22
EP2810074A1 (en) 2014-12-10
JP2015507193A (ja) 2015-03-05
JP2019113564A (ja) 2019-07-11
MX2014008762A (es) 2014-08-27
US9945869B2 (en) 2018-04-17
CN104105966B (zh) 2016-10-26
BR112014017630A8 (pt) 2017-07-11
US10761097B2 (en) 2020-09-01
EP2810074B1 (en) 2017-08-23
US20140342382A1 (en) 2014-11-20
BR112014017630A2 (es) 2017-06-20
KR20140128314A (ko) 2014-11-05
JP6502441B2 (ja) 2019-04-17
CA2859268A1 (en) 2013-08-08
CN106443016B (zh) 2019-08-13
WO2013113663A1 (en) 2013-08-08
JP6678792B2 (ja) 2020-04-08
US20180328942A1 (en) 2018-11-15
HK1202921A1 (en) 2015-10-09

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