AR063125A1 - Ensayo elisa para la deteccion de vegf - Google Patents

Ensayo elisa para la deteccion de vegf

Info

Publication number
AR063125A1
AR063125A1 ARP070104393A AR063125A1 AR 063125 A1 AR063125 A1 AR 063125A1 AR P070104393 A ARP070104393 A AR P070104393A AR 063125 A1 AR063125 A1 AR 063125A1
Authority
AR
Argentina
Prior art keywords
vegf
antibody
biological sample
binds
detection
Prior art date
Application number
Other languages
English (en)
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of AR063125A1 publication Critical patent/AR063125A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/22—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against growth factors ; against growth regulators
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
    • G01N33/57496—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites involving intracellular compounds
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/475—Assays involving growth factors

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Endocrinology (AREA)
  • Oncology (AREA)
  • Hospice & Palliative Care (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La actividad del factor de crecimiento endotelial vascular (VEGF) en el torrente sanguíneo de un paciente o en otra muestra biologica puede servir como índice de diagnostico y pronostico para el cáncer, diabetes, afecciones cardíacas y otras patologías. Los métodos de ensayo ELISA de tipo ôsándwichö que utilizan anticuerpos y los kits de deteccion de VEGF como antígeno se proporcionan para detectar los tipos de niveles de VEGF en muestras biologicas de modelos animales y pacientes humanos y pueden ser utilizados como índice de diagnostico y pronostico. Reivindicacion 1: Un método para la deteccion de formas selectivas del factor de crecimiento endotelial vascular (VEGF) (VEGF110+) en una muestra biologica que comprende los siguientes pasos: (a) poner en contacto e incubar la muestra biologica con un reactivo de captura inmovilizado en un soporte solido, en el que el reactivo de captura es un anticuerpo que reconoce el mismo epítopo que el anticuerpo 5C3 contra VEGF humano. Dicho anticuerpo monoclonal se une específicamente a residuos del VEGF humano superiores a 110; (b) separar la nuestra biologica de los reactivos de captura inmovilizados; (c) poner en contacto el complejo ôreactivo de captura-molécula dianaö inmovilizado con un anticuerpo detectable que se una a los dominios de union del receptor KDR y/o FLT1 del VEGF o que se une a un epítopo en VECF110; y (d) medir el nivel del VEGF110+ unido a los reactivos de captura a través un método de deteccion del anticuerpo detectable.
ARP070104393 2006-10-04 2007-10-03 Ensayo elisa para la deteccion de vegf AR063125A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US82820306P 2006-10-04 2006-10-04

Publications (1)

Publication Number Publication Date
AR063125A1 true AR063125A1 (es) 2008-12-30

Family

ID=39301650

Family Applications (2)

Application Number Title Priority Date Filing Date
ARP070104393 AR063125A1 (es) 2006-10-04 2007-10-03 Ensayo elisa para la deteccion de vegf
ARP170100313A AR107561A2 (es) 2006-10-04 2017-02-08 Ensayo elisa para la detección de vegf

Family Applications After (1)

Application Number Title Priority Date Filing Date
ARP170100313A AR107561A2 (es) 2006-10-04 2017-02-08 Ensayo elisa para la detección de vegf

Country Status (25)

Country Link
US (5) US20080227119A1 (es)
EP (3) EP3103812B1 (es)
JP (1) JP6025294B2 (es)
KR (2) KR101551984B1 (es)
CN (2) CN103454434B (es)
AR (2) AR063125A1 (es)
AU (1) AU2007319654B2 (es)
BR (1) BRPI0715239A2 (es)
CA (1) CA2663012C (es)
DK (2) DK2069798T3 (es)
ES (2) ES2584322T3 (es)
HK (3) HK1168361A1 (es)
HR (1) HRP20140675T1 (es)
HU (1) HUE030082T2 (es)
IL (1) IL197374A (es)
MX (2) MX2009003562A (es)
NZ (1) NZ613646A (es)
PL (2) PL2457929T3 (es)
PT (1) PT2069798E (es)
RS (1) RS53387B (es)
RU (2) RU2517301C2 (es)
SI (2) SI2457929T1 (es)
TW (2) TWI541251B (es)
WO (1) WO2008060777A2 (es)
ZA (1) ZA200901518B (es)

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CN102435743B (zh) * 2011-09-15 2014-10-15 北京健平九星生物医药科技有限公司 一种酶联检测试剂盒及其制备方法
CN102426240B (zh) * 2011-09-19 2014-10-15 北京健平金星生物科技有限公司 一种酶联检测试剂盒及其制备方法
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SG11201403927XA (en) 2012-01-13 2014-08-28 Genentech Inc Biological markers for identifying patients for treatment with vegf antagonists
WO2013113663A1 (en) * 2012-02-01 2013-08-08 F. Hoffmann-La Roche Ag Method for the detection of a binding partner of a multispecific binder
CN102645530B (zh) * 2012-04-06 2014-06-18 上海蓝怡科技有限公司 甲状腺素酶联免疫体外诊断试剂盒中酶结合物稀释液的制备方法
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RU2622005C2 (ru) * 2015-11-24 2017-06-08 Федеральное государственное бюджетное учреждение "Государственный научный центр дерматовенерологии и косметологии" Минздрава России (ФГБУ ГНЦДК Минздрава России) СПОСОБ ПОЛУЧЕНИЯ СЕЛЕКТИВНОГО ИММУНОСОРБЕНТА ДЛЯ УДАЛЕНИЯ АНТИТЕЛ-IgG К ДЕСМОГЛЕИНУ 3 ТИПА ИЗ СЫВОРОТКИ КРОВИ БОЛЬНЫХ ПУЗЫРЧАТКОЙ
CN105510595A (zh) * 2015-12-01 2016-04-20 邹检平 基于量子点CdTe检测人血清中VEGF浓度的试剂盒及其使用方法
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CN107167588A (zh) * 2017-03-28 2017-09-15 马杰 用于检测血清中血管内皮生长因子的抗体及试剂盒
RU2732013C1 (ru) * 2019-12-11 2020-09-10 Федеральное бюджетное учреждение науки "Государственный научный центр прикладной микробиологии и биотехнологии" Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека (ФБУН ГНЦ ПМБ) Способ сенсибилизации планшета для иммуноферментного анализа нерастворимыми белковыми антигенами
CN111044734A (zh) * 2019-12-30 2020-04-21 上海复星长征医学科学有限公司 一种用于检测血管内皮生长因子的检测试剂盒及其制备方法和使用方法
KR102676458B1 (ko) 2021-05-20 2024-06-19 대한민국 보툴리눔 독소 a에 특이적인 항체, 이를 생산하는 하이브리도마 세포주, 및 이를 이용한 보툴리눔 독소 a 진단 키트
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Also Published As

Publication number Publication date
WO2008060777A3 (en) 2008-07-10
RU2009116611A (ru) 2010-11-10
RU2517301C2 (ru) 2014-05-27
TW200823233A (en) 2008-06-01
US20160033517A1 (en) 2016-02-04
EP3103812A1 (en) 2016-12-14
JP6025294B2 (ja) 2016-11-16
ZA200901518B (en) 2010-05-26
ES2584322T3 (es) 2016-09-27
KR101720887B1 (ko) 2017-03-28
EP2069798A2 (en) 2009-06-17
KR101551984B1 (ko) 2015-09-09
CN103454434A (zh) 2013-12-18
IL197374A0 (en) 2009-12-24
SI2457929T1 (sl) 2016-09-30
CA2663012A1 (en) 2008-05-22
PT2069798E (pt) 2014-06-24
US8449885B2 (en) 2013-05-28
TWI541251B (zh) 2016-07-11
RS53387B (en) 2014-10-31
AR107561A2 (es) 2018-05-09
US20130045495A1 (en) 2013-02-21
AU2007319654A1 (en) 2008-05-22
CA2663012C (en) 2017-06-27
CN101523220B (zh) 2016-09-21
HRP20140675T1 (hr) 2014-10-10
IL197374A (en) 2016-11-30
PL2457929T3 (pl) 2016-12-30
ES2470682T3 (es) 2014-06-24
HK1168361A1 (zh) 2012-12-28
EP2069798B1 (en) 2014-04-16
MX342791B (es) 2016-10-12
DK2069798T3 (da) 2014-06-16
US9625462B2 (en) 2017-04-18
RU2014104113A (ru) 2015-08-10
NZ613646A (en) 2015-03-27
MX2009003562A (es) 2009-04-15
CN101523220A (zh) 2009-09-02
BRPI0715239A2 (pt) 2013-06-25
US20170276683A1 (en) 2017-09-28
EP3103812B1 (en) 2021-07-28
SI2069798T1 (sl) 2014-07-31
DK2457929T3 (en) 2016-09-05
HUE030082T2 (en) 2017-04-28
EP2457929B1 (en) 2016-06-08
KR20090091695A (ko) 2009-08-28
TWI429654B (zh) 2014-03-11
WO2008060777A2 (en) 2008-05-22
EP2457929A1 (en) 2012-05-30
US20100255515A1 (en) 2010-10-07
HK1232236A1 (zh) 2018-01-05
TW201406778A (zh) 2014-02-16
JP2010506171A (ja) 2010-02-25
PL2069798T3 (pl) 2014-09-30
KR20150038444A (ko) 2015-04-08
US20080227119A1 (en) 2008-09-18
CN103454434B (zh) 2016-08-10
US9146245B2 (en) 2015-09-29
AU2007319654B2 (en) 2013-09-12
HK1130090A1 (en) 2009-12-18

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