MX349003B - Metodo para la deteccion del asociado de union libre de un aglutinante multiespecifico. - Google Patents

Metodo para la deteccion del asociado de union libre de un aglutinante multiespecifico.

Info

Publication number
MX349003B
MX349003B MX2014007095A MX2014007095A MX349003B MX 349003 B MX349003 B MX 349003B MX 2014007095 A MX2014007095 A MX 2014007095A MX 2014007095 A MX2014007095 A MX 2014007095A MX 349003 B MX349003 B MX 349003B
Authority
MX
Mexico
Prior art keywords
detection
binding partner
free binding
multispecific antibody
multispecific binder
Prior art date
Application number
MX2014007095A
Other languages
English (en)
Other versions
MX2014007095A (es
Inventor
Stubenrauch Kay-Gunnar
Wessels Uwe
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of MX2014007095A publication Critical patent/MX2014007095A/es
Publication of MX349003B publication Critical patent/MX349003B/es

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding

Abstract

La presente invención se refiere a un método in vitro para la determinación de la presencia y/o cantidad de un antígeno de un anticuerpo multiespecífico en una muestra, mientras el antígeno a ser detectado puede estar específicamente unido por una primera especificidad del anticuerpo multiespecífico, caracterizado porque comprende los pasos de; -incubar la muestra que comprende el anticuerpo multiespecífico, anticuerpo unido al anticuerpo multiespecífico y el antígeno libre con un anticuerpo anti-idiotípico que específica se une a una segunda especificidad del anticuerpo multiespecífico, que es diferente de la primera especificidad de unión, -agotar el complejo de anticuerpo anti-idiotípico-anticuerpo multiespecífico de la muestra antes de la determinación de la presencia y/o la cantidad de asociado de unión libre, y -determinar la presencia y/o la cantidad de antígeno libre en la muestra con anticuerpo multiespecífico agotado.
MX2014007095A 2011-12-19 2012-12-18 Metodo para la deteccion del asociado de union libre de un aglutinante multiespecifico. MX349003B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP11194350 2011-12-19
PCT/EP2012/075991 WO2013092611A2 (en) 2011-12-19 2012-12-18 Method for the detection of free binding partner of a multispecific binder

Publications (2)

Publication Number Publication Date
MX2014007095A MX2014007095A (es) 2014-07-22
MX349003B true MX349003B (es) 2017-07-06

Family

ID=47429825

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2014007095A MX349003B (es) 2011-12-19 2012-12-18 Metodo para la deteccion del asociado de union libre de un aglutinante multiespecifico.

Country Status (11)

Country Link
US (1) US9671394B2 (es)
EP (1) EP2795335B1 (es)
JP (1) JP6203747B2 (es)
KR (1) KR20140099277A (es)
CN (1) CN104040350B (es)
BR (1) BR112014011535A2 (es)
CA (1) CA2853917A1 (es)
HK (1) HK1201920A1 (es)
MX (1) MX349003B (es)
RU (1) RU2633488C2 (es)
WO (1) WO2013092611A2 (es)

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EP3066469B1 (en) * 2013-11-05 2017-10-11 Roche Diagnostics GmbH A method for determining the total amount and/or concentration of an analyte in the presence of a binding molecule as well as kits, compositions and uses relating thereto
EP3119805A1 (en) * 2014-03-20 2017-01-25 Wellstat Diagnostics, LLC Antibodies and methods for the detection of cell death
CN107003307B (zh) 2014-12-18 2020-04-07 豪夫迈·罗氏有限公司 降低干扰的方法
JP2020530554A (ja) * 2017-07-20 2020-10-22 シートムエックス セラピューティクス，インコーポレイテッド 活性化抗体の特性を定性的および／または定量的に分析する方法およびその使用
CN109358192B (zh) * 2018-02-08 2020-05-12 中国科学院上海药物研究所 一种去除抗药抗体检测样品中游离药物的装置和方法、该装置的制备方法及应用
EP3805757A4 (en) 2018-06-04 2022-03-23 Chugai Seiyaku Kabushiki Kaisha METHOD FOR DETECTING A COMPLEX
WO2020229378A1 (en) * 2019-05-13 2020-11-19 F. Hoffmann-La Roche Ag Interference-suppressed pharmacokinetic immunoassay
CN117805397A (zh) * 2024-02-29 2024-04-02 军科正源（北京）药物研究有限责任公司 检测游离vegf的方法

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Also Published As

Publication number Publication date
JP6203747B2 (ja) 2017-09-27
CA2853917A1 (en) 2013-06-27
MX2014007095A (es) 2014-07-22
EP2795335A2 (en) 2014-10-29
CN104040350B (zh) 2016-05-18
RU2014128510A (ru) 2016-02-10
BR112014011535A2 (pt) 2017-05-09
WO2013092611A3 (en) 2013-10-17
WO2013092611A2 (en) 2013-06-27
JP2015502547A (ja) 2015-01-22
KR20140099277A (ko) 2014-08-11
HK1201920A1 (zh) 2015-09-11
CN104040350A (zh) 2014-09-10
RU2633488C2 (ru) 2017-10-12
US20150072359A1 (en) 2015-03-12
EP2795335B1 (en) 2017-09-06
US9671394B2 (en) 2017-06-06

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