MX2016016133A - Métodos y composiciones para modificar un locus dirigido. - Google Patents

Métodos y composiciones para modificar un locus dirigido.

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Publication number
MX2016016133A
MX2016016133A MX2016016133A MX2016016133A MX2016016133A MX 2016016133 A MX2016016133 A MX 2016016133A MX 2016016133 A MX2016016133 A MX 2016016133A MX 2016016133 A MX2016016133 A MX 2016016133A MX 2016016133 A MX2016016133 A MX 2016016133A
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MX
Mexico
Prior art keywords
cell
methods
polynucleotide
compositions
recognition site
Prior art date
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MX2016016133A
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English (en)
Other versions
MX385689B (es
Inventor
Frendewey David
Droguett Gustavo
Auerbach Wojtek
KUNO Junko
M Valenzuela David
Gagliardi Anthony
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Regeneron Pharma
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Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of MX2016016133A publication Critical patent/MX2016016133A/es
Publication of MX385689B publication Critical patent/MX385689B/es

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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2207/00—Modified animals
    • A01K2207/15—Humanized animals
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    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/072—Animals genetically altered by homologous recombination maintaining or altering function, i.e. knock in
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A01K2227/00—Animals characterised by species
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    • A01K2267/03—Animal model, e.g. for test or diseases
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    • A01K2267/0387—Animal model for diseases of the immune system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0278—Knock-in vertebrates, e.g. humanised vertebrates
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C12N2015/8527—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic for producing animal models, e.g. for tests or diseases
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    • C12N2810/00—Vectors comprising a targeting moiety

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  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Se proporcionan métodos y composiciones para modificar uno o más loci objetivo en una célula. Tales métodos comprenden proporcionar una célula que comprende un primer polinucleótido que codifica un primer marcador de selección unido operativamente a un primer promotor activo en la célula, en donde el primer polinucleótido comprende, además, un primer sitio de reconocimiento para un primer agente nucleasa. Un primer agente nucleasa se introduce en una célula, en donde el primer agente nucleasa induce una mella o una ruptura de doble hebra en el primer sitio de reconocimiento. En la célula se introduce, además, un primer vector de direccionamiento que comprende un primer polinucleótido inserto flanqueado por un primer y un segunda brazo de homología que corresponden a un primer y un segundo sitio objetivo situados en suficiente proximidad al primer sitio de reconocimiento. Después, se identifica al menos una célula que comprende en su genoma el primer polinucleótido inserto integrado en el locus objetivo.
MX2016016133A 2014-06-06 2015-06-05 Métodos y composiciones para modificar un locus dirigido. MX385689B (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462008832P 2014-06-06 2014-06-06
US201462017916P 2014-06-27 2014-06-27
PCT/US2015/034503 WO2015188109A1 (en) 2014-06-06 2015-06-05 Methods and compositions for modifying a targeted locus

Publications (2)

Publication Number Publication Date
MX2016016133A true MX2016016133A (es) 2017-08-04
MX385689B MX385689B (es) 2025-03-18

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Family Applications (1)

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MX2016016133A MX385689B (es) 2014-06-06 2015-06-05 Métodos y composiciones para modificar un locus dirigido.

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US (4) US10106820B2 (es)
EP (2) EP3152312B1 (es)
JP (2) JP6688231B2 (es)
KR (1) KR102374379B1 (es)
CN (2) CN113215196B (es)
AU (1) AU2015269187B2 (es)
BR (1) BR112016028564A2 (es)
CA (1) CA2950173C (es)
CY (1) CY1122897T1 (es)
DK (1) DK3152312T3 (es)
ES (2) ES3019688T3 (es)
HR (1) HRP20200529T1 (es)
HU (1) HUE049776T2 (es)
IL (1) IL249042B (es)
LT (1) LT3152312T (es)
MX (1) MX385689B (es)
NZ (1) NZ727481A (es)
PL (1) PL3152312T3 (es)
PT (1) PT3152312T (es)
RS (1) RS60359B1 (es)
RU (1) RU2704283C9 (es)
SG (2) SG11201609634YA (es)
SI (1) SI3152312T1 (es)
SM (1) SMT202000264T1 (es)
WO (1) WO2015188109A1 (es)

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